Hee Young Kang

Ajou University, Sŏul, Seoul, South Korea

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Publications (109)249.85 Total impact

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    ABSTRACT: Development of vitiligo-like hypopigmentary lesions associated with topical imiquimod has been reported. We hypothesized that mode of action of imiquimod in melanocytes may include triggering of apoptosis resulted in loss of cells, which may be a possible mechanism of imiquimod-induced hypopigmentary lesions. Therefore, we investigated whether imiquimod induces apoptosis of human melanocytes and also whether it modulates expression of apoptosis-related molecules in human melanocytes. Imiquimod treatment induced apoptosis of melanocytes, which was observed by TUNEL assay and Hoechst 33258 staining. Imiquimod-induced apoptosis was further shown by measuring mitochondrial membrane potential in melanocytes. The apoptotic activity of imiquimod was associated with caspase-3, Bcl-2 and mitogen-activated protein kinase expression in melanocytes. These results indicated that imiquimod induces apoptosis of melanocytes. These findings may provide a clue to understand pathogenesis of imiquimod-induced vitiligo-like hypopigmentary lesions.
    Archives for Dermatological Research 05/2010; 302(4):301-6. DOI:10.1007/s00403-009-1012-0 · 2.27 Impact Factor
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    ABSTRACT: Psoriasis is a relatively common disorder in children and can be triggered by an upper respiratory tract infection. The aim of this study was to compare the clinical features of psoriasis in children and adult. In addition, we evaluated the relationship between anti-streptolysin O (ASO) titers and the clinical features of psoriasis. A total of 30 childhood psoriasis patients and 30 adult psoriasis patients were evaluated. Childhood psoriasis had a facial predominance when compared with the adult psoriasis. The childhood psoriasis patients with high ASO titers had guttate psoriasis more frequently than patients with normal ASO titers. In children with plaque-type psoriasis, psoriasis area and severity index score was increased in the high ASO titer group than normal ASO titer group. In conclusion, if the children with psoriasis show increased ASO titer, the physician should pay attention to the worsening of the psoriasis. Furthermore, early treatment of streptococcal infections might be beneficial in childhood psoriasis.
    Archives for Dermatological Research 05/2010; 302(4):295-9. DOI:10.1007/s00403-009-1025-8 · 2.27 Impact Factor
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    ABSTRACT: Melasma is a frequent pigmentary disorder caused by abnormal melanin deposits in the skin. In vivo reflectance confocal microscopy (RCM) is a repetitive imaging tool that provides real-time images of the skin at nearly histological resolution. As melanin is the strongest endogenous contrast in human skin, pigmentary disorders are the most suitable candidates for RCM examination but RCM features of melasma have never been reported. This study investigates the pilot use of RCM in melasma to provide a set of well-described morphological criteria with histological correlations. RCM images were acquired from melasma skin and compared to adjacent control skin in 26 patients. Skin biopsies were obtained from eight patients. In the epidermis, RCM showed in all patients a significant increase in hyperrefractile cobblestoning cells. These cells corresponded to hyperpigmented basal keratinocytes in histology. In six patients, dendritic cells corresponding to activated melanocytes were also found in the epidermis. In the dermis, RCM identified in nine patients plump bright cells corresponding to melanophages. Interestingly, for a given patient, the topographic distribution of melanophages in melasma lesions was very heterogeneous. RCM also showed a significant increase in solar elastosis and blood vessels in the dermis. RCM is a non-invasive technique that detects pigmentary changes in melasma at a cellular level resolution. Therefore, RCM provides an innovative way to classify melasma by pigment changes.
    Experimental Dermatology 05/2010; 19(8):e228-33. DOI:10.1111/j.1600-0625.2009.01057.x · 4.12 Impact Factor
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    ABSTRACT: The histological findings associated with idiopathic guttate hypomelanosis (IGH) are hyperkeratosis, an atrophic epidermis, and flattened rete ridges. In addition, a decreased melanin content and reduced numbers of melanocytes are reported features. However, there are few recent studies that have been published on the histopathology of IGH and no comparative studies are available on the skin lesions and perilesional skin of patients with IGH. The goals of this study were to identify the clinical and histopathological features of IGH and determine their correlation. We evaluated the clinical features and the histopathological differences between the skin lesions and the perilesional skin in patients with IGH. A clinical survey was carried out on 47 patients with IGH. Specimens from skin lesions and perilesional skin were stained with hematoxylin-eosin, Fontana-Masson, MART-1, and NKI/beteb. We also studied the ultrastructure of four cases. About 30% of the patients had their initial lesions prior to 20 years of age. The arm was the most commonly affected site (53%). Histologically, we found hyperkeratosis in 18 cases (38.3%), but epidermal atrophy was present in only five cases (10.6%), and flattened rete ridges in seven cases (14.9%) compared to the normal skin. Epidermal atrophy was more frequently found at nonsun-exposed areas. The IGH lesions demonstrated decreased melanin pigment and reduced numbers of melanocytes by NKI/beteb and MART-1. The ultrastructural evaluation showed degenerative melanocytes and decreased melanosomes. One specimen had normal melanocytes with decreased melanosomes. Idiopathic guttate hypomelanosis is a disorder with multifactorial etiology; its pathogenesis may depend on various factors such as patient age and sun-exposure. Histopathologically, hyperkeratosis was frequently found; however, the other characteristic findings such as epidermal atrophy and flattened rete ridges were relatively rare.
    International journal of dermatology 02/2010; 49(2):162-6. DOI:10.1111/j.1365-4632.2009.04209.x · 1.23 Impact Factor
  • Archives of dermatology 01/2010; 146(1):81-6. DOI:10.1001/archdermatol.2009.335-a · 4.31 Impact Factor
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    ABSTRACT: In vivo reflectance confocal microscopy (RCM) is a non-invasive, repetitive imaging tool that provides real-time images at nearly cellular histological resolution. Application of this technology to skin imaging during the last decade has been a great advance in dermatology. As melanin is the strongest endogenous contrast in human skin, pigmentary disorders caused by abnormal amounts of melanin in the skin could be the most suitable candidates for RCM examination. This article reviewed the RCM applications in the characterization and management of pigmentary disorders. The application of RCM in pigmentary disorders has been expanded to describe hyper- and hypopigmentary disorders as well as pigmented skin tumors. The great advantages of non-invasive and repetitive examination of RCM may provide its usefulness not only in the diagnosis and management of pigmentary disorders, but also in researching pathogenesis of pigmentary disorders.
    Experimental Dermatology 11/2009; 19(3):233-9. DOI:10.1111/j.1600-0625.2009.00983.x · 4.12 Impact Factor
  • Sue Kyung Kim, Jin Soon Hwang, Hee Young Kang
    International journal of dermatology 09/2009; 48(8):909-10. DOI:10.1111/j.1365-4632.2009.04165.x · 1.23 Impact Factor
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    ABSTRACT: Livedo reticularis (LR) is a skin disorder characterized by a reddish-blue mottling of the skin in a "fishnet" reticular pattern. The aim of this study was to investigate the major histopathological characteristics of LR and determine whether the histopathology of LR is more common in whitish areas or in the erythematous areas. A clinical survey was carried out that included 16 patients with LR. The histopathological features were investigated with immunostains for factor VIII-related antigen, as well as routine hematoxylin-eosin. There were no significant differences in thrombi, arterial obliteration, or RBC aggregates between the central blanched area and the peripheral erythematous areas. The computer-assisted image analyses of factor VIII-related antigen-stained sections revealed no significant difference in the number of dermal blood vessels and telangiectasia between the areas studied. There were nine cases that showed one or more of the histopathological features of LR in either the central blanched areas or the peripheral erythematous areas. Our findings suggest that multiple punch biopsies should be performed in patients suspected of LR including both the peripheral erythematous and central whitish areas to increase the diagnostic yield.
    Journal of Cutaneous Pathology 08/2009; 36(12):1275-8. DOI:10.1111/j.1600-0560.2009.01282.x · 1.56 Impact Factor
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    ABSTRACT: In this study, we identified extended-spectrum beta-lactamase (ESBL) and plasmid-mediated AmpC beta-lactamase which were associated with 16S rRNA methylase gene on the conjugative plasmid. Among 82 clinical isolates of Enterobacteriaceae that carry 16S rRNA methylase gene (64 strains, armA, and 18 strains, rmtB), bla(SHV-12) was detected either alone or combined with bla(DHA-1), bla(CTX-M-3), and bla(CTX-M-14) in 30 strains carrying armA and 6 strains carrying rmtB. The bla(CTX-M-3) was detected in 13 of 64 strains carrying armA but no strains carrying rmtB. Whereas bla(CTX-M-14) was detected in 15 of 18 strains carrying rmtB but only 2 of 64 strains carrying armA. Overall, bla(SHV-12) and bla(CTX-M-14) was the most common ESBL gene which was associated with armA and rmtB, respectively. In addition, we found that bla(CTX-M-3) localized with armA on the same IncL/M plasmid and bla(CTX-M-14) localized with rmtB on the same IncA/C plasmid. Restriction fragment length polymorphism of conjugative plasmids and pulsed-field gel electrophoresis of genomic DNAs revealed that intercellular horizontal transfer of conjugative plasmid and clonal transmission have been occurred at the same time.
    The Journal of Microbiology 03/2009; 47(1):68-75. DOI:10.1007/s12275-008-0158-3 · 1.53 Impact Factor
  • Hee Young Kang, Chang Kwon Hwangbo
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    ABSTRACT: In this study, the authors propose transparent conductive oxide layer as a new absorber for extreme ultraviolet lithography mask. The optical constant of indium tin oxide (ITO) at 13.5 nm is calculated and the extinction coefficient of ITO is found to be 1.55 times higher than that of TaN, resulting in higher absorption in the absorbing stack. Using an ITO layer in the absorber stack enables to design the attenuated phase-shift masks with smaller height difference of only 32.1 nm than the conventional TaN absorber stack of about 80 nm between high reflecting and absorbing stacks in extreme ultraviolet lithography, indicating that the geometric shadow effect can be significantly reduced.
    Journal of vacuum science & technology. B, Microelectronics and nanometer structures: processing, measurement, and phenomena: an official journal of the American Vacuum Society 02/2009; 27(1-27):58 - 60. DOI:10.1116/1.3043471 · 1.36 Impact Factor
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    ABSTRACT: Topical retinoids have been used in the treatment of pigmentary disorders such as melasma, actinic lentigines, and postinflammatory hyperpigmentation. This article evaluates the clinical efficacy and tolerability of retinoid treatment for pigmentary disorders through an evidence-based approach. We searched the MEDLINE and The Cochrane Library databases using the keywords 'retinoid' combined with 'melasma,' 'lentigines,' or 'postinflammatory hyperpigmentation.' For each study, the methodology and outcomes were assessed according to specific criteria. There is fair evidence to support the use of topical tretinoin as a monotherapy in the treatment of melasma as well as in the treatment of lentigines (grade B). Adverse effects of topical retinoids are quite frequent, and include local skin irritation, erythema, and peeling, and their severity is mild to moderate. There is evidence to support the use of topical tretinoin in a fixed, triple-combination therapy (hydroquinone 4%/tretinoin 0.05%/fluocinolone acetonide 0.01%) for the treatment of melasma (grade B). There is poor evidence (grade C) to support the use of combination formulations for the treatment of lentigines, and large, randomized, double-blind, controlled trials are needed to further evaluate their use for this indication. In conclusion, there is evidence to support the use of topical retinoids as monotherapy or in combination with other topical agents in the treatment of pigmentary disorders.
    American Journal of Clinical Dermatology 02/2009; 10(4):251-60. DOI:10.2165/00128071-200910040-00005 · 2.52 Impact Factor
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    Hee Young Kang, Min Chul Shin
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    ABSTRACT: To report a case of epithelial ingrowth treatment by surgical excision of epithelial tissues and intracameral 5-fluorouracil injection.
    Journal of the Korean Ophthalmological Society 01/2009; 50(2). DOI:10.3341/jkos.2009.50.2.313
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    ABSTRACT: Plasmid-mediated quinolone resistance (PMQR) genes: qnr, aac(6')-Ib-cr, and qepA were investigated among 153 armA and 51 rmtB-positive transconjugants and their 204 clinical isolates of Enterobacteriaceae. Overall, qnrB4 and aac(6')-Ib-cr genes were identified in 52.3% (63 K. pneumoniae, 10 E. coli, 4 E. cloacae, and 3 E. aerogenes) and 24.8% (16 K. pneumoniae, 8 E. coli, 6 S. marcescens, 4 E. cloacae, 3 C. freundii and 1 K. oxytoca) of 153 armA-positive isolates, respectively. Four isolates of K. pneumoniae and two isolates of E. coli positive for armA co-harbored both qnrB4 and aac(6')-Ib-cr. The qepA gene was detected in 11.8% (5 E. coli and 1 K. pneumoniae) of 51 rmtB-positive clinical isolates and their transconjugants. Southern hybridization confirmed the co-localization of qepA and rmtB on a large conjugative plasmid of size between 90 to 170 kb. Inc replicon typing showed that qnrB4/6, aac(6')-Ib-cr, and qepA genes were principally disseminated by IncFIIAs, IncL/M, and IncF plasmids, respectively. This study constitutes the first report of the three known PMQR genes among the 16S rRNA methylase producing Enterobacteriaceae isolates of human origin from Korea.
    Journal of Bacteriology and Virology 01/2009; 39(3). DOI:10.4167/jbv.2009.39.3.173
  • Jungmin Kim, Hee Young Kang, Yeonhee Lee
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    ABSTRACT: From water samples collected monthly between 2000 and 2001 from the Han River in Seoul, sixteen strains of Escherichia coli which confer resistance to at least 10 kinds of antimicrobial agents were isolated. From these isolates, 2 kinds of extended-spectrum beta-lactamases (ESBLs) and one plasmid-mediated AmpC beta-lactamase were detected; CTX-M-14 from 10 isolates, TEM-52 from 5 isolates, and CMY-1 from one isolate. Class 1 integron gene cassettes, such as aadA1, dfr12-orfF-aadA2, and dfr17-aadA5, were also detected and the integrons are the same as those found in E. coli isolated from swine, poultry, and humans in Korea. The result of this study indicated the importance of river water as a reservoir for antimicrobial resistance genes and resistant bacteria.
    The Journal of Microbiology 11/2008; 46(5):478-81. DOI:10.1007/s12275-008-0150-y · 1.53 Impact Factor
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    ABSTRACT: Background: ISCR elements are a group of insertion elements that belong to the IS91 family. They are implicated in the movement of many resistancegenes including qnr gene. Their association with extended spectrum ß-lactamase (ESBL) and plasmid-mediated AmpC-type ß-lactamase (pACBL) genes is of particular concern regarding the further spread ofthese genes. Therefore, the purpose of this study was to determine the prevalence of common ISCR elements among the qnr-positive clinical isolates of Enterobacteriaceae and to investigate the association between ISCR, qnr, ESBL and/pACBL genes. Methods:A total of 141 qnr-positive isolates of Enterobacteriaceae isolated at a University Hospital were analyzed. The presence of ISCR elements, bla DHA-1, bla SHV, armA, and bla CTX-M beta-lactamase genes was determined by PCR assays, followed by direct DNA sequencing. PCR primers for ISCR1, qnrB, and bla DHA-1 were used to map the ISCR1- qnrB - bla DHA-1 region to investigate their association. Results: Of the 141 clinical strains tested, 82 (58.1%), 10 (7.0%), and 21 (14.9%) were positive for ISCR1, ISCR2, and both ISCR1 and ISCR2, respectively. No isolate harbored ISCR3 element. The prevalence of ISCR1 as well as ISCR2 was highest among the Klebsiella pneumoniae, followed by Escherichia coli. ISCR1, qnrB4/6, and bla DHA-1 were simultaneously identified in 75 and 35 of the clinical isolates and their transconjugants, respectively. By PCR mapping, approximately 3.5-Kbp region between ISCR1 and qnrB4/6 and a 5.9-Kbp region between qnrB4/6 and bla DHA-1 were amplified and identified by sequencing. Conclusions: The results suggest widespread distribution of ISCR elements and a strong association between ISCR1, qnrB4/6, and bla DHA-1 genes in Enterobacteriaceae. This work also identifies the association between ISCR1, qnrB6, and bla DHA-1 genes, which has never been reported before. The simultaneous mobilization of qnrB4/6 and bla DHA-1 genes seems to have occurred by recombinatorial events mediated by ISCR1 elements.
    Infectious Diseases Society of America 2008 Annual Meeting; 10/2008
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    ABSTRACT: Background: Recently, genes conferring resistance against aminoglycosides, extended-spectrum beta-lactams, and fluoroquinolones (FQs) have been reported on a same transferable plasmid. In our previous study, transferable plasmids which confer a high-level resistance to amikacin and carry 16s rRNA methylase genes such as armA and rmtB were related to FQs resistance. The aim of this study was to investigate plasmid-mediated FQs resistance determinants which are associated with 16s rRNA methylase genes among Enterobacteriaceae. Methods: Screening for plasmid-mediated FQs resistance determinants such as qnrA, qnrB, qnrS, and qepA genes were performed by PCR in a total of 193 Enterobacteriaceae isolates carrying 16s rRNA methylase genes. Incompatibility group of transferable plasmids was examined by a PCR-based inc/replicon typing. The genetic context of qepA was characterized by a PCR mapping. Results: Among 146 armA-positive isolates of Enterobacteriaceae, qnrB genes were detected in 80 strains which carried IncFIIAs or Inc(unidentified) plasmids. Among 47 rmtB-positive isolates of Enterobacteriaceae, qepA gene was detected in 4 isolates (1 Klebsiella pneumoniae and 3 Escherichia coli) which carried IncF plasmid. PCR mapping study revealed that the structure of region containing qepA was very similar to blaTEM-1, rmtB, ISCR3 and, qepA carrying plasmid previously reported from Japan. Conclusions: These findings warn us that novel combinations of transferable resistance determinants continue to spread around the world and could seriously undermine therapeutic regimens with beta-lactams, FQs and aminoglycosides. This is the first report of plasmid-mediated qepA gene in Enterobacteriaceae from Korea. This study also identifies qepA in a clinical isolates of K. pneumoniae, which was never been reported before.
    Infectious Diseases Society of America 2008 Annual Meeting; 10/2008
  • Journal- Korean Physical Society 09/2008; 53(3). DOI:10.3938/jkps.53.1638 · 0.43 Impact Factor
  • Sue Kyung Kim, Hee Young Kang
    European journal of dermatology: EJD 09/2008; 18(5):596. DOI:10.1684/ejd.2008.0486 · 1.95 Impact Factor
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    ABSTRACT: Screening of 368 consecutive nonreplicate clinical isolates of Enterobacteriaceae resistant to nalidixic acid and at least one extended-spectrum beta-lactam revealed the presence of qnrA, qnrB, and qnrS determinants, and identified novel qnrB variants, in Citrobacter freundii isolates. This study also revealed, for the first time, the linkage of qnrB, armA, and extended-spectrum and/or AmpC-type beta-lactamase genes on large conjugative plasmids.
    Antimicrobial Agents and Chemotherapy 09/2008; 52(11):4159-62. DOI:10.1128/AAC.01633-07 · 4.45 Impact Factor
  • Joo Hee Ahn, Sun Hee Jin, Hee Young Kang
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    ABSTRACT: We have observed that lipopolysaccharide (LPS) induces pigmentation in melanocytes and in this study have examined whether these responses are mediated by the p38 mitogen-activated protein kinase (MAPK) signaling pathway. LPS appears to stimulate the pigmentation of melanocytes and cultured skin. LPS was found to induce the expression of microphthalmia-associated transcription factor (MITF) and tyrosinase protein in cells. Stimulation of melanocytes with LPS led to time dependent phosphorylation of p38 MAPK. Furthermore, p38 MAPK functionally regulated the LPS-induced melanin formation in melanocytes; a p38 MAPK inhibitor, SB203580, almost completely attenuated the LPS-mediated up-regulation of melanin synthesis and induction of MITF and tyrosinase expression. These findings indicate that activation of p38 MAPK plays an important role in LPS-induced melanogenesis by up-regulating MITF and tyrosinase expression.
    Archives for Dermatological Research 08/2008; 300(6):325-9. DOI:10.1007/s00403-008-0863-0 · 2.27 Impact Factor

Publication Stats

936 Citations
249.85 Total Impact Points

Institutions

  • 2003–2015
    • Ajou University
      • Department of Dermatology
      Sŏul, Seoul, South Korea
  • 2014
    • Korea Photonics Technology Institute
      Sŏul, Seoul, South Korea
  • 2003–2014
    • Kyungpook National University
      • Department of Microbiology
      Daikyū, Daegu, South Korea
  • 2008–2013
    • Inha University
      • Department of Physics
      Chemulpo, Incheon, South Korea
  • 2012
    • Kim's Eye Hospital
      Sŏul, Seoul, South Korea
  • 2011
    • Gyeongsang National University
      Shinshū, Gyeongsangnam-do, South Korea
  • 2006–2008
    • Kyungpook National University Hospital
      Sŏul, Seoul, South Korea
  • 2004–2006
    • Chungbuk National University
      • Division of Life Sciences
      Tyundyu, North Chungcheong, South Korea
    • Eulji University
      Daiden, Daejeon, South Korea