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Archives of Dermatology 11/2005; 141(10):1311-6. · 3.89 Impact Factor
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The Journal of Dermatology 10/2005; 32(9):765-6. · 1.49 Impact Factor
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Journal of the American Academy of Dermatology 08/2005; 53(1):177-8. · 3.99 Impact Factor
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ABSTRACT: We report two cases of lichen planus pigmentosus (LPP) that developed in a unilateral linear pattern. The patients presented with unilateral linear brown macules on the extremities. Skin biopsy showed orthokeratosis, basal hydropic degeneration with scarce lymphohistiocytic infiltrates, and numerous melanophages in both patients. These patients, to the best of our knowledge, are the first cases of LPP presenting with a linear pattern. LPP should be considered in the differential diagnosis of linear hyperpigmented skin lesions.
Journal of Korean Medical Science 03/2004; 19(1):152-4. · 0.99 Impact Factor
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Archives for Dermatological Research 01/2003; 294(9):426-9. · 2.28 Impact Factor
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ABSTRACT: We investigated the expression of ryanodine receptors (RyRs) in cultured human melanocytes with immunocytochemistry and reverse transcriptase–polymerase chain reaction. With the use of a monoclonal antibody, RyR immunoreactivity was detected in the cytoplasm of melanocytes, and was further confirmed by RT-PCR assay. The PCR products were cut with restriction enzymes specific for each RyR isoform. Using the RyR1-specific restriction enzyme SacI yielded fragments of 300, 100, and 130 base pairs, consistent with the expression of RyR1 isoforms. The function of RyR in Ca2+ signaling was investigated using single-cell fura-2 imaging. Ryanodine (1 to ∼100 μM) induced significant elevation of cytoplasmic Ca2+ in single human melanocytes in a dose-dependent manner. The ryanodine-induced [Ca2+]i increase was inhibited by neomycin. Furthermore, ryanodine inhibited proliferation and stimulated pigmentation of human melanocytes. This study demonstrates that the RyR1 isoform is expressed in cultured human melanocytes, and suggests that the RyR may be involved in regulating the intracellular Ca2+ responses involved in proliferation and pigmentation of cultured human melanocytes. J. Cell. Physiol. 185:200–206, 2000. © 2000 Wiley-Liss, Inc.
Journal of Cellular Physiology 10/2000; 185(2):200 - 206. · 3.87 Impact Factor
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ABSTRACT: The mechanism of an endothelin-1- (ET-1-) induced intracellular Ca2+ ([Ca2+]i) increase and the receptor subtype(s) responsible for this effect in single human melanocytes were studied using fura-2/AM.
ET-1 induced a transient increase in [Ca2+]i in a concentration-dependent manner. The transient [Ca2+]i increase was followed by a sustained plateau level of [Ca2+]i which was higher than the initial [Ca2+]i level. IRL-1620, a specific ET-B receptor agonist, increased [Ca2+]i in a dose-dependent manner. BQ-788, a specific ET-B receptor antagonist, abolished the ET-1-induced [Ca2+]i increase, but BQ-123, a specific ET-A receptor antagonist, failed to prevent it. U73122, an inhibitor of phospholipase C
(PLC), inhibited the ET-1-induced [Ca2+]i rise in a dose-dependent manner. Prior depletion of intracellular Ca2+ stores with thapsigargin, an inhibitor of Ca2+-ATPase of the endoplasmic reticulum, abolished the ET-1-induced Ca2+ transient, whereas removal of extracellular Ca2+ with EGTA eliminated the sustained rise. These results suggest that in cultured human melanocytes the binding of ET-1 to
ET-B receptors and the subsequent activation of PLC mediate ET-1-induced [Ca2+]i increase. The transient [Ca2+]i increase is attributed to mobilization of Ca2+ from inositol 1,4,5-trisphosphate-sensitive intracellular Ca2+ stores, and the sustained [Ca2+]i level may be related to the influx of extracellular Ca2+.
Pflügers Archiv - European Journal of Physiology 12/1997; 435(3):350-356. · 4.46 Impact Factor
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European journal of dermatology: EJD 16(5):582-3. · 2.53 Impact Factor
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ABSTRACT: Pili multigemini is a rare developmental defect of hair follicles. It usually involves the beard of an adult and the scalp in children, but there have been no reported cases on the back. We report 3 cases of extensive pili multigemini over the entire back of healthy males. All cases were found incidentally. The direct microscopic examination of one hair follicle showed that it consisted of more than twenty shafts, which had rough surfaces and brush-like bulbs. The histological examination revealed that complicated follicular structures formed as many as 23 to even 36 hair shafts. Lots of hairs were surrounded by each inner root sheath and had one common outer root sheath. We suggest this nevoid is pili multigemini over the back.
European journal of dermatology: EJD 15(2):99-101. · 2.53 Impact Factor
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European journal of dermatology: EJD 17(4):343-5. · 2.53 Impact Factor
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European journal of dermatology: EJD 17(4):347-8. · 2.53 Impact Factor
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European journal of dermatology: EJD 17(6):537-8. · 2.53 Impact Factor
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ABSTRACT: Segmented heterochromia is a pigmentary disorder characterized by alternating dark and light segments on each hair shaft. Our aim was to observe the ultrastructure of hair shafts and bulbs to understand the pathogenesis of the disease. Biopsy specimens including normal or diseased hair follicles were prepared for transmission electron microscopy. In dark segments, dense and ellipsoidal melanosomes were noted while small, round ones were found in the light segments. Two groups of melanosomes lined up on the matrix, one of which was composed of compact ellipsoidal melanosomes, and the other was composed of melanosomes with irregular sizes and shapes. Melanocytes seemed to be under necrosis, apoptosis, or dark cell transformation. Langerhans cells were found in the bulb. Two kinds of melanosomes were produced in the same hair bulb simultaneously. Degenerating melanocytes might produce deformed melanosomes. Langerhans cells might be involved in the death of melanocytes. It is unknown how either group of melanosomes is adopted and transferred to the hair cortex producing the characteristic pattern of pigmentation.
European journal of dermatology: EJD 14(6):388-90. · 2.53 Impact Factor
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ABSTRACT: We report a 30-year-old female patient with a history of hyperpigmented macules of the face since the age of 7 years. The clinical appearance, brown macules in multiple segmental or grouped patterns, was suggested to be agminated lentigines. However, histologic examination of the pigmented macule revealed scattered melanocytes within the dermis. We diagnosed this lesion as a bilateral type of nevus of Ota presenting as agminated lentigines. The differential diagnosis from acquired bilateral nevus of Ota-like macules was made.
European journal of dermatology: EJD 13(2):205-6. · 2.53 Impact Factor
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ABSTRACT: Acute graft versus host disease (GVHD) is characterized by a selective epithelial inflammation that can affect the skin, digestive tract, and liver. Development of pigmentary abnormalities can be observed in sites where acute cutaneous GVHD has occurred, and usually consists of hyperpigmented spots. We observed atypical pigmetary changes consisting of a combination of hyper- and hypopigmentation, so called leukomelanoderma, in a young allogeneic bone marrow recipient who suffered repetitive acute GVHD. The histopathological examinations showed features of a post-inflammatory process. Because keratinocytes produce inflammatory cytokines including tumor necrosis factor (TNF)-alpha and interleukin (IL)-1alpha, which may be implicated in the inflammatory phenomena seen in acute GVHD, we studied whether these inflammatory cytokines might be implicated in these pigmentary changes. The cytokines tested were IL-1alpha, IL-2, TNF-alpha and IL-10. The expression of TNF-alpha increased in the hyperpigmented skin relative to normal and hypopigmented skin. While TNF-alpha was variably distributed in proportion to different degrees of pigmentation, other molecules were detected at minimal levels in all samples. This observation may indicate that the production of TNF-alpha by epidermal microenvironment may be involved in postinflammatory pigmentary changes.
European journal of dermatology: EJD 14(3):146-9. · 2.53 Impact Factor
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European journal of dermatology: EJD 18(5):596. · 2.53 Impact Factor
