Antonella Anastasi

Università degli Studi di Torino, Torino, Piedmont, Italy

Are you Antonella Anastasi?

Claim your profile

Publications (28)63.32 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: The ability of fungi to break down complex carbon sources makes them of vital importance in both the generation and application of compost. This paper illustrates the diversity and functions of the mycoflora of both a green compost (made solely thermophilically from plant debris) and a vermicompost (made mesophilically by the action of earthworms on plant and animal wastes). The soil dilution plate technique was applied on 3 media (PDA, CMC, PDA plus cycloheximide) and at 3 incubation temperatures (24°C, 37°C and 45°C) to isolate and identify fungal entities. Enzymatic activities (amylase, cellulase, chitinase, esterase, ligninase, pectinase, phosphatase, plastic degradation and xylanase) of most species from both composts were evaluated with a semiquantitative method on 15 substrates. There were substantial qualiquantitative differences in the species composition of the two composts. A total of 193 entities were isolated: 54 from green compost only, 77 from vermicompost only, 62 from both. This taxonomic diversity was reflected in the metabolic potential. Amilase, cellulase, protease and esterase activities were significantly higher in the green compost. Lignin and plastic polymer degradation were significantly higher in the vermicompost. Structural and functional characterization of this kind is of assistance in determining both the most appropriate application of composts and their hygienic quality.
    Compost science & utilization 07/2013; 12(2):185-191. DOI:10.1080/1065657X.2004.10702179 · 0.58 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The degree of decolorization of Poly R-478, a highly recalcitrant anthraquinone dye, by three basidiomycetes belonging to Polyporales isolated from compost was investigated together with the enzymes involved. Decolorization tests in two liquid cultures, one with a simple mycelium inoculum, the other with an inoculum of mycelium grown on straw, resulted in 70% and 87% decolorization respectively in 7 days. However, the efficiency did not increase significantly in the presence of the lignocellulose substrate. The three strains produced laccase and/or manganese peroxidase activity during the decolorization, whereas lignin peroxidase activity was not observed. Previous growth on straw enhanced the synthesis of ligninolytic enzymes, though there was no correlation between enzyme activity and decolorization. The three fungi can be proposed as promising candidates for the treatment of colored industrial effluents and probably for soils contaminated by complex polymers, such as polycyclic aromatic hydrocarbons.
    Compost science & utilization 07/2013; 14(4):284-289. DOI:10.1080/1065657X.2006.10702298 · 0.58 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The marine environment is characterized by high salinity and exerts a strong selective pressure on the biota, favouring the development of halo-tolerant microorganisms. Part of this microbial diversity is made up of fungi, important organisms from ecological and biotechnological points of view. In this study, for the first time, the qualitative and quantitative composition of the mycoflora associated to leaves, rhizomes, roots and matte of the seagrass Posidonia oceanica was estimated. A total of 88 fungal taxa, mainly belonging to Ascomycota, were identified by morphological and molecular methods. The most represented genera were Penicillium, Cladosporium and Acremonium. Most of the species (70) were selectively associated with one district; only two species (Penicillium chrysogenum var. chrysogenum and P. janczewskii) were isolated from all the districts. Moreover the capability to produce laccases, peroxidases and tannases by 107 fungal isolated by the different districts of P. oceanica was carried out. These results show that the mycoflora associated to P. oceanica is very rich and characterized by fungi able to produce ligninolytic enzymes and tannases useful to degrade and detoxify lignocellulose residues in presence of high salt concentrations. These fungi, hence, may play important ecological roles in marine environments but can also be very useful in different biotechnological areas.
    New Biotechnology 02/2013; 30(6). DOI:10.1016/j.nbt.2013.01.010 · 2.90 Impact Factor
  • Antonella Anastasi · Valeria Tigini · Giovanna Cristina Varese ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Fungi play a major role in all ecosystems as decomposers, symbionts, and pathogens. Their morphological, physiological, and reproductive strategies make them especially suited for terrestrial habitats. This chapter intends to describe their multifaceted role in the biodegradation of natural and xenobiotic compounds taking into account the specific features of different ecophysiological groups, focusing both on saprotrophs, as wood, litter, and soil fungi, and on mycorrhizal fungi. Successful use of fungi in soil bioremediation depends on a comprehensive knowledge of their ecology, physiology, and enzymology. Each section will start from the description of the role of the different groups in nature; then it will go on exploring the potential for degradation of the major organic pollutants in soil, in order to provide a comprehensive overview of the potential use of fungi as bioremediators.
    Fungi as Bioremediators, 01/2013: pages 29-49;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The biosorption potential of three fungal waste-biomasses (Acremonium strictum, Acremonium sp. and Penicillium sp.) from pharmaceutical companies was compared with that of a selected biomass (Cunninghamella elegans), already proven to be very effective in dye biosorption. Among the waste-biomasses, A. strictum was the most efficient (decolorization percentage up to 90% within 30 min) with regard to three simulated dye baths; nevertheless it was less active than C. elegans which was able to produce a quick and substantial decolorization of all the simulated dye baths (up to 97% within 30 min). The biomasses of A. strictum and C. elegans were then tested for the treatment of nine real exhausted dye baths. A. strictum was effective at acidic or neutral pH, whereas C. elegans confirmed its high efficiency and versatility towards exhausted dye baths characterised by different classes of dyes (acid, disperse, vat, reactive) and variation in pH and ionic strength. Finally, the effect of pH on the biosorption process was evaluated to provide a realistic estimation of the validity of the laboratory results in an industrial setting. The C. elegans biomass was highly effective from pH 3 to pH 11 (for amounts of adsorbed dye up to 1054 and 667 mg of dye g(-1) biomass dry weight, respectively); thus, this biomass can be considered an excellent and exceptionally versatile biosorbent material.
    Water 12/2012; 4(4):770-784. DOI:10.3390/w4040770 · 1.43 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A combined biological process was investigated for effective textile wastewater treatment. The process consisted of a first step performed by selected fungal biomasses, mainly devoted to the effluent decolourisation, and of a subsequent stage by means of activated sludge, in order to reduce the remaining COD and toxicity. In particular, the treatment with Trametes pubescens MUT 2400, selected over nine strains, achieved very good results in respect to all parameters. The final scale-up phase in a moving bed bioreactor with the supported biomass of the fungus allowed to verify the effectiveness of the treatment with high volumes. Despite promising results, further steps must be taken in order to optimize the use of these biomasses for a full exploitation of their oxidative potential in textile wastewater treatment.
    Bioresource Technology 07/2012; 123:106-11. DOI:10.1016/j.biortech.2012.07.026 · 4.49 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Treatment of industrial wastewaters represents an actual and spread topic. In fact, the in-use techniques are not able to completely degrade all the pollutants, present in the effluents. At the moment, other approaches are under investigation, but they often have some drawbacks in terms of economical and environmental sustainability. A strain of Bjerkandera adusta MUT 2295, previously selected for its capability to degrade several industrial model dyes, has been tested towards real industrial wastewaters, coming from textile and pharmaceutical industries. The efficacy of the treatment was monitored, following the decolourisation percentage (DP) and the modification of other parameters as the chemical oxygen demand (COD). The effect of the fungal immobilisation on 4 inert supports was investigated in order to select the best one in terms of biomass production and enzymatic activity. The efficiency of the immobilized biomass was assessed toward a textile effluent, comparing it with a free-cell treatment.
    3rd International Conference on Industrial Biotechnology (IBIC); 01/2012
  • F. Spina · A. Romagnolo · A. Anastasi · V. Tigini · V. Prigione · G.C. Varese ·
    [Show abstract] [Hide abstract]
    ABSTRACT: The treatment of real textile wastewater was carried out by two Bjerkandera adusta strains (MUT 2295 and MUT 3060), showing a significant intrinsic variability in terms of decolorization yields (75% vs 43%). Fungal efficiency was compared with the biological technique already used in a wastewater treatment plant: fungi were active mainly towards the color whereas activated sludge towards the COD. The fungal process was optimized focusing on the biomass immobilization on four inert supports, using static or agitated colonization phases. The best results were obtained in agitation on polyurethane foam cubes; the mycelium homogenously colonized the carriers and resulted more active and stable during time. The so-immobilized fungus was used to treat a real textile effluent and the final process yield was compared to a free pellet approach. The supported biomass maintained the same efficiency than the free one; considering the technical advantages, a fungal treatment by means of a immobilized fungal mycelium showed great applicative potential. In order to provide a method more suitable from an applicative point of view, a twostep biological process was set up: the fungus and the activated sludge were able to synergically work. At the end of the process, both color (more than 60%) and COD (around 50%) were reduced in comparison to the initial values.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The fungal biodiversity in its overall is mostly still unknown and the ecological role of these organisms, particularly in some border ecosystems, is often underestimated. This study aims to give both an overview of the state of the art and to present new data on the mycodiversity in some peculiar environments as rocks, beach sand, and water in Italy. Particularly, rock fungi are here reported from high mountain peaks, sea cliffs, and monuments; sand associated fungi from beach ecosystems in Puglia and Ligurian coasts; marine fungi associated with the endemic seagrass of the Mediterranean Posidonia oceanica L.; aquatic hyphomycetes (Ingoldian fungi) from both streams in the Ticino Natural Park and lentic water in Lago Maggiore; fungi from the water distribution system in Turin. Ecological and evolutive considerations are put forward.
    Plant Biosystems 12/2011; 145(4-4):978-987. DOI:10.1080/11263504.2011.633117 · 1.92 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study a selected fungal strain, Bjerkandera adusta (Willdenow) P. Karsten MUT 3060, was tested in different culture conditions to assess its real potential for bioremediation of textile wastewaters in terms of both decolourisation and detoxification. The fungus efficiently decolourised (colour removal up to 96%) four simulated wastewaters that mimic the recalcitrance of real ones for pH values, concentration of dyes, additives and salts. In the culture condition with the lowest N content, the decolourisation was coupled with an important detoxification of two simulated effluents, underlining the important influence of the cultural medium composition not only on the degradation but also on the detoxification of industrial wastes. In the other cases, despite an extensive decolourisation, no detoxification was observed. The fungus was further tested against a real effluent, collected from a wastewater treatment plant before and after the tertiary treatment (ozonation) to compare the two technologies in terms of chemical and toxicological parameters. The fungal treatment, although less efficient than ozonation, caused a good decolourisation of the effluent, with colour values within the threshold limits of the Italian law; both the fungal and the ozone treatment caused a detoxification, but only towards one of the three organisms used for the ecotoxicological tests. These results underline the crucial importance of the ecotoxicological analysis in assessing the applicability of a wastewater treatment.
    New Biotechnology 09/2011; 29(1):38-45. DOI:10.1016/j.nbt.2011.08.006 · 2.90 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The effect of pre-treatments on the composition of Cunninghamella elegans biomass and on its biosorption yields in the treatment of simulated textile wastewaters was investigated. The inactivated biomass was subjected to physical treatments, such as oven drying and lyophilisation, and chemical treatments using acid or alkali. The wastewater colour, COD and toxicity variations were evaluated. The lyophilisation sped up the biosorption process, whereas the chemical pre-treatment changed the affinity of biomass for different dyes. The alkali per-treated biomass achieved the highest COD reduction in the treatment of alkali wastewaters, probably because no release of alkali-soluble biomass components occurred under the alkaline pH conditions. Accordingly, only the acid pre-treated biomass decreased the COD of the acidic effluent. The ecotoxicity test showed significant toxicity reduction after biosorption treatments, indicating that decolourisation corresponds to an actual detoxification of the treated wastewaters. Fourier transform infrared spectroscopy, differential scanning calorimetry and thermogravimetric analyses of biomasses allowed highlighting their main chemical and physical properties and the changes induced by the different pre-treatments, as well as the effect of the chemical species adsorbed from wastewaters.
    Applied Microbiology and Biotechnology 12/2010; 90(1):343-52. DOI:10.1007/s00253-010-3010-8 · 3.34 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Basidiomycetes are essential in forest ecology, being deeply involved in wood and litter decomposition, humification, and mineralization of soil organic matter. The fungal oxidoreductases involved in these processes are today the focus of much attention with a view to their applications. The ecological role and potential biotechnological applications of 300 isolates of Basidiomycetes were assessed, taking into account the degradation of model dyes in different culture conditions and the production of oxidoreductase enzymes. The tested isolates belong to different ecophysiological groups (wood-degrading, litter-degrading, ectomycorrhizal, and coprophilous fungi) and represent a broad systematic and functional biodiversity among Basidiomycetes occurring in deciduous and evergreen forests of northwest Italy (Piedmont Region). The high number of species tested and the use of different culture conditions allowed the investigation of the degradation activity of several novel species, neglected to date. Oxidative enzyme activities varied widely among all ecophysiological groups and laccases were the most commonly detected enzymes. A large number of isolates (86%), belonging to all ecophysiological groups, were found to be active against at least one model dye; the wood-degrading fungi represented the most efficient group. Noteworthily, also some isolates of litter-degrading and ectomycorrhizal fungi achieved good decolorization yield. The 25 best isolates were then tested against nine industrial dyes commonly employed in textile industries. Three isolates of Bjerkandera adusta efficiently decolorized the dyes on all media and can be considered important candidates for application in textile wastewater treatment.
    Antonie van Leeuwenhoek 11/2010; 98(4):483-504. DOI:10.1007/s10482-010-9466-9 · 1.81 Impact Factor
  • L. Panno · S. Voyron · A. Anastasi · G. C. Varese ·

    Journal of Biotechnology 11/2010; 150(supp. 1):383-384. DOI:10.1016/j.jbiotec.2010.09.479 · 2.87 Impact Factor
  • A. Anastasi · F. Spina · V. Prigione · V. Tigini · G. C. Varese ·

    Journal of Biotechnology 11/2010; 150:52-52. DOI:10.1016/j.jbiotec.2010.08.138 · 2.87 Impact Factor
  • Antonella Anastasi · Valeria Prigione · Giovanna Cristina Varese ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Twenty-five basidiomycetes belonging to 17 species and ascribable to different eco-physiological groups were screened for their ability to decolorize 9 commercially important industrial dyes comprising a variety of anthraquinonic, azoic and phtalocyanin chromophores. The influence of the culture medium, particularly its C:N ratio, on decolourisation capacity was considered on solid substrate. Three strains of Bjerkandera adusta performed the highest decolourisation yields being able to degrade all dyes on all media and to produce a wide spectrum of oxidative enzyme activities. Hence, B. adusta strains were selected for further experiments in liquid cultures together with other 6 fungi that resulted effective in the decolourisation of the largest number of molecules in the broadest spectrum of cultural conditions. Particularly B. adusta MUT 3060 was found very effective (decolourisation percentage over 90%) in the treatment of simulated effluents composed of single and mixed dyes at high concentration (1000 mg/l). Peroxidase activity dependent (up to 362 U/l) and independent from manganese (up to 57 U/l) were detected during the decolourisation process. The Lemna minor toxicity test showed a significant reduction of toxicity after the fungal treatment indicating that decolourisation corresponded to an actual detoxification of the wastewater.
    Journal of hazardous materials 05/2010; 177(1-3):260-7. DOI:10.1016/j.jhazmat.2009.12.027 · 4.53 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Twelve basidiomycetes were investigated for their ability to degrade 13 industrial dyes and to treat four model wastewaters from textile and tannery industry, defined on the basis of discharged amounts, economic relevance and representativeness of chemical structures of the contained dyes. The best degradation yields were recorded for one strain of Bjerkandera adusta able to completely decolourise most of the dyes and to decolourise and detoxify three simulated wastewaters, showing a significant physiological versatility which is very useful for application purposes. The effects of different nutrient sources were investigated in order to optimize the yields of decolourisation and detoxification. Manganese-peroxidase and manganese-independent peroxidase were the only recorded enzymatic activities. In order to evaluate its true bioremediation potential, this strain was packed in a fixed-bed bioreactor, for treatment of large volumes of a real wastewater. The fungus resulted effective during 10 cycles of decolourisation, remaining active for a very long period, in non-sterile conditions.
    Bioresource Technology 05/2010; 101(9):3067-75. DOI:10.1016/j.biortech.2009.12.067 · 4.49 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Six mitosporic fungi belonging to five species (Aspergillus flavus var. flavus, Aspergillus ochraceus, Cladosporium cladosporioides, Penicillium glabrum and Penicillium verrucosum) were selected from a screening on 258 fungal strains as the most promising for their ability to remove 2 model dyes in solid conditions. Hence they were tested in liquid conditions for their ability to decolourise 3 model dyes and 9 industrial dyes widely used in the textile industry. The influence of the culture medium, particularly its carbon:nitrogen ratio, on biomass development and decolourisation capacity was considered. All the strains were able to grow in the dyed media and displayed various degrees of decolourisation according to the dye and culture medium. The decolourisation was due to biosorption phenomena. Aspergillus ochraceus performed the highest decolourisation yield being able to remove all dyes over 90%. This strain was also found very effective, both in the living and inactivated form, against simulated effluents that mimicked the recalcitrance of real wastewaters being composed of ten different dyes at high concentration (1,000ppm), in saline solution.
    World Journal of Microbiology and Biotechnology 08/2009; 25(8):1363-1374. DOI:10.1007/s11274-009-0023-5 · 1.78 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Heavy metals represent an important ecological and health hazard due to their toxic effects and their accumulation throughout the food chain. Conventional techniques commonly applied to recover chromium from tanning wastewaters have several disadvantages whereas biosorption has good metal removal performance from large volume of effluents. To date most studies about chromium biosorption have been performed on simulated effluents bypassing the problems due to organic or inorganic ligands present in real industrial wastewaters that may sequestrate the Cr(III) ions. In the present study a tanning effluent was characterized from a mycological point of view and different fungal biomasses were tested for the removal of Cr(III) from the same tanning effluent in which, after the conventional treatments, Cr(III) amount was very low but not enough to guarantee the good quality of the receptor water river. The experiments gave rise to promising results with a percentage of removed Cr(III) up to 40%. Moreover, to elucidate the mechanisms involved in biosorption process, the same biomasses were tested for Cr(III) removal from synthetic aqueous solutions at different Cr(III) concentrations.
    Bioresource Technology 03/2009; 100(11):2770-6. DOI:10.1016/j.biortech.2009.01.002 · 4.49 Impact Factor

  • A Textbook of Molecular Biotechnology, Edited by Chauhan AK, Varma A, 01/2009: pages 957-993; I.K. International, New Delhi., ISBN: 9789380026374
  • Antonella Anastasi · Tiziana Coppola · Valeria Prigione · Giovanna C Varese ·
    [Show abstract] [Hide abstract]
    ABSTRACT: A consortium of three basidiomycetes isolated from compost was investigated for pyrene degradation in soil microcosms. Pyrene concentration, glucose and ammonium evolution, moisture content, ligninolytic enzyme activities and phytotoxicity (germination index) on Lepidium sativum L. seeds were monitored. The fungal consortium grown on straw was found able to efficiently colonize soil and remove about 56 out of 100 mg kg(-1) of soil dry weight of pyrene in 28 days; in the meantime the germination index increased indicating a reduction of phytotoxicity. A glucose supply after 2 weeks was found useful to ensure fungal growth and activity; maintenance of moisture content below 70% allowed a good aeration of the system and improved degradation rates. Enzymatic assays showed that laccase and manganese independent peroxidase activity could have played a role in the degradation process.
    Journal of hazardous materials 11/2008; 165(1-3):1229-33. DOI:10.1016/j.jhazmat.2008.10.032 · 4.53 Impact Factor