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ABSTRACT: An alginate lyase-producing bacterim, designated AlyHP32T, was isolated from the gut of sea urchin (Hemicentrotus pulcherrimus) obtained from the South Sea, Republic of Korea. Cells of strain AlyHP32T were Gram reaction-negative, motile with a single polar flagellum. The strain grew in 1-6 % (w/v) NaCl (optimum 2-4 %) and at 4-30 oC (optimum 25 oC). Phylogenetic analysis based on sequences of the 16S rRNA gene and five housekeeping genes (atpA, pyrH, recA, rpoA and rpoD) revealed that strain AlyHP32T belonged to the genus Vibrio and formed a compact clade with Vibrio splendidus group. However, DNA-DNA hybridization and fingerprints using the repetitive primers BOX and REP indicated that the strain AlyHP32T was distinguished from the closely related Vibrio species. The major fatty acids were summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and C16:0. The DNA G+C content was 44.1 mol%. The predominant quinone was menaquinone 8 (MK-8). Based on genotypic, phenotypic and DNA-DNA hybridization analysis, the name Vibrio hemicentroti sp. nov. (type strain AlyHP32T = KCTC 32085T = DSM 26178T) is proposed for this novel taxon.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2013; · 2.11 Impact Factor
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ABSTRACT: Two yellow-pigmented, rod-shaped, non-motile, Gram-reaction-negative and aerobic bacterial strain, designated strains KYW560T and KYW563T, were isolated from seawater collected from Gwangyang Bay, Republic of Korea. The isolates required sea salts for growth. Flexirubin-type pigments were absent. Common major cellular fatty acids (>5 % of total) of the two strains were C16:0, C18:0, iso-C15:0, anteiso-C15:0, iso-C15:1 G, iso-C17:0 3-OH and summed feature 3 (C16:1 ω6c and/or C16:1 ω7c). Strain KYW560T also contained iso-C15:0 3-OH and C20:1 ω9c as main fatty acids. The main polar lipids were phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. The predominant isoprenoid quinone is MK-6. The DNA G+C contents of strains KYW560T and KYW563T are 41.0±0.7 and 38.3±0.4 mol% (mean±SD of three determinations), respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolates belong to the family Flavobacteriaceae, and were related to the genus Algibacter. Based on data from a study using a polyphasic taxonomic approach, it is proposed that the isolates represent new species belonging to the genus Algibacter, for which the name Algibacter agarivorans sp. nov. (type strain KYW560T =KCTC 23855T =JCM 18285T) and Algibacter agarolyticus sp. nov. (type strain KYW563T =KCTC 23857T =JCM 18275T) are proposed. Reclassification of Marinivirga aestuarii as Algibacter aestuarii comb. nov. was also proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2013; · 2.11 Impact Factor
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ABSTRACT: A Gram-positive, aerobic, coccoid-rod shaped, non-motile, catalase- and oxidase-positive bacterium, designated strain KJW98, was isolated from the marine sediment of Karwar jetty, west coast of India. The strain was β-haemolytic, non-endospore-forming and grew with 0-8.5% (w/v) NaCl, at 15-48°C and at pH 6.5-9.0, with optimum growth with 0.5% (w/v) NaCl, at 42°C and at pH 7.0-8.0. Phylogenetic analyses based on 16S rRNA and gyrB gene sequences showed that strain KJW98 forms a lineage within the genus Bhargavaea. The G+C content of the genomic DNA was 55 mol%. The DNA-DNA relatedness values of strain KJW98 with B. beijingensis DSM 19037, B. cecembensis LMG 24411 and B. ginsengi DSM 19038 were 43.2, 39 and 26.5%, respectively. The major fatty acids were anteiso-C (37.7%), iso-C (19.7%), anteiso-C (17.0%) and iso-C (11.1%). The predominant menaquinone was MK-8 and the cell-wall peptidoglycan was of A4α type with L-lysine as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The phenotypic, genotypic and DNA-DNA relatedness data indicate that strain KJW98 should be distinguished from the members of the genus Bhargavaea, for which the name Bhargavaea indica sp. nov. is proposed with the type strain KJW98 (=KCTC 13583 =LMG 25219).
The Journal of Microbiology 02/2013; 51(1):36-42. · 1.10 Impact Factor
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ABSTRACT: A Gram-stain-negative, non-motile, strictly aerobic, yellow-pigmented bacterium, designated strain 5G38, was isolated from a field cultivated with Chinese cabbage in Korea. The strain grew at 5-40°C and at pH 6.0-8.0. 16S rRNA gene sequence analysis revealed that strain 5G38 represented a distinct lineage within the family Sphingobacteriaceae and showed the highest 16S rRNA gene sequence similarity of 95.2% with Pedobacter koreensis WPCB189, followed by Pedobacter agri PB92 (94.6%), Pedobacter suwonensis 15-52 (94.4%), Pedobacter rhizosphaerae 01-96 (94.4%), Pedobacter sandarakinus DS-27 (94.4%), and Nubsella zeaxanthinifaciens TDMA-5 (94.3%). Strain 5G38 formed monophyletic clade with Nubsella zeaxanthinifaciens in the cluster comprised of species of the genus Pedobacter. Chemotaxonomic characteristics of the novel strains, including DNA G+C content of genomic DNA (37.0 mol%), the predominant respiratory quinine (MK-7), and the major fatty acids which were iso-C, summed feature 3 (comprising C ω7c and/or iso-C 2-OH) and iso-C 3-OH, are similar to those of the genus Pedobacter. However, the novel strains can be distinguished from the other species of Pedobacter by physiological properties. The name Pedobacter namyangjuensis sp. nov. is therefore proposed for strain 5G38 (KACC 13938 =NBRC 107692) as the type strain. Furthermore, the reclassification of Nubsella zeaxanthinifaciens as Pedobacter zeaxanthinifaciens comb. nov. is proposed.
The Journal of Microbiology 02/2013; 51(1):25-30. · 1.10 Impact Factor
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ABSTRACT: A Gram-staining-negative, non-spore-forming, non-gliding and rod-shaped bacterial strain KYW566T, was isolated from seawater of the Suncheon Bay, Korea, and its taxonomic position was investigated by using a polyphasic study. The cells contained MK-6 as the only respiratory quinone and contained iso-C15:0 (13.8 %), iso-C16:0 3-OH (13.1 %), anteiso-C15:0 (9.3 %), iso-C15:0 3-OH (8.7 %), iso-C15:1 G (6.3 %) and summed feature 3 (C16:1w7c and/or C16:1w6c) (6.0 %) as the major fatty acids. The DNA G+C content of strain KYW566T was 41.6±0.8 mol% (mean±SD of three determinations). A phylogenetic tree based on 16S rRNA gene sequences showed that strain KYW566T forms an evolutionary lineage within the radiation enclosing the members of the genus Lutibacter with Lutibacter flavus IMCC1507T as its nearest neighbor (96.7 % sequence similarity). A number of phenotypic characteristics distinguished strain KYW566T from described members of the genus Lutibacter. On the basis of the evidences presented in this study, strain KYW566T represents a novel species, for which the name Lutibacter agarilyticus sp. nov. is proposed. The type strain is KYW566T (=KCTC 23842T= JCM 18281T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2013; · 2.11 Impact Factor
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ABSTRACT: An orange-coloured, rod-shaped, Gram-reaction-negative and aerobic bacterial strain, designated KYW589T, was isolated from seawater collected from Gwangyang Bay, Republic of Korea. The isolate required sea salts for growth. Gliding motility was observed. Flexirubin-type pigments were absent. Major cellular fatty acids (>10 % of total) were iso-C15:0, iso-C17:0 3-OH, iso-C15:1 G, C16:0, iso-C15:0 3-OH and C18:0. The main polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The predominant isoprenoid quinone is MK-6. The DNA G+C content is 38.6±0.7 mol% (mean±SD of three determinations). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KYW589T belongs to the family Flavobacteriaceae, and was related to the genus Algibacter. Based on data from a study using a polyphasic taxonomic approach, it is proposed that strain KYW589T represents a new species belonging to the genus Algibacter, for which the name Algibacter aquimarinus sp. nov. is proposed. The type strain is KYW589T (=KCTC 23928T = JCM 18287T). Reclassification of Pontirhabdus pectinivorans to the genus Algibacter, as Algibacter pectinivorans comb. nov. (type strain JC2675T =KACC 14153T =JCM 17107T) was proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2012; · 2.11 Impact Factor
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ABSTRACT: An orange-coloured and slender rod shaped bacterium, designated strain PSC32T, was isolated from the gut microflora of a mussel collected from the Gwangyang bay, South Sea (Republic of Korea). Cells were Gram-reaction-negative, strictly aerobic, catalase- and oxidase-positive. The major fatty acids were iso-C15:0, iso-C17:0 3-OH, iso-C15:1 G and iso-C15:0 3-OH. The only isoprenoid quinone of strain PSC32T was MK-6 and the DNA G+C content was 36.9 mol%. Phosphatidylethanolamine, one unidentified aminolipid and two unidentified polar lipids were found as major polar lipids. A phylogenetic tree based on 16S rRNA gene sequences showed that strain PSC32T forms an evolutionary lineage within the genus Aquimarina and is closely related to A. spongiae A6T (97.0 % sequence similarity) and to other members of the genus Aquimarina (94.4-96.5 % sequence similarity). Genomic DNA-DNA relatedness between strain PSC32T and A. spongiae A6T was 40.7 %. A number of phenotypic characteristics distinguished strain PSC32T from described members of the genus Aquimarina. On the basis of the evidences presented in this study, strain PSC32T represents a novel species, for which the name Aquimarina gracilis sp. nov. is proposed. The type strain is PSC32T (=KCTC 23301T = JCM 17453T). Emended description of Aquimarina spongiae is also proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 08/2012; · 2.11 Impact Factor
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ABSTRACT: Two orange-colored, rod-shaped, Gram-reaction-negative, aerobic bacterial strains devoid of flagellar and gliding motility, designated strains KYW371T and KS18 were isolated from seawater collected from Gwangyang Bay, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains belonged to the family Flavobacteriaceae; and that strain KYW371T was most closely related to Algibacter mikhailovii LMG 23988T (96.7 %, sequence similarity), Pontirhabdus pectinovorans JC2675T (96.3 %), Postechiella marina M091T (95.6 %) and Hyunsoonleella jejuensis CNU004T (95.3 %). The 16S rRNA gene sequence similarity (98.8 %) and DNA-DNA relatedness (78.1 %) between strains KYW371T and KS18 indicated that these two strains represented a single species. The predominant cellular fatty acids of strain KYW371T were iso-C15:1 G (22.8 %), iso-C15:0 (20.1 %), iso-C15:0 3-OH (15.2 %) and iso-C17:0 3-OH (13.2 %). Flexirubin-type pigments were absent. MK-6 was the only isoprenoid quinone and the DNA G+C content was 34.8-36.6 mol%. Data from a polyphasic taxonomy study suggested that the isolates represent a novel genus and species in the family Flavobacteriaceae, for which the name Marinivirga aestuarii gen. nov., sp. nov. is proposed. The type strain is KYW371T (=KCTC 23449T =JCM 17452T). Emended descriptions of the genera Hyunsoonleella, Jejuia and Pontirhabdus and Hyunsoonleella jejuensis, Jejuia pallidilutea and Pontirhabdus pectinivorans are also proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 08/2012; · 2.11 Impact Factor
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ABSTRACT: Two yellow-pigmented, Gram reaction-negative strains, designated 01SU5-PT and 03SU3-PT, were isolated from the freshwater of Woopo wetland, Republic of Korea. Both strains are aerobic, non-motile and catalase-negative. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two isolates belong to the genus Sphingopyxis, showing the highest level of sequence similarity with respect to Sphingopyxis witflariensis W-50T (95.4-95.7 %). The two isolates shared 99.4 % sequence similarity. DNA-DNA hybridization between the isolates and the type strain of Sphingopyxis witflariensis clearly suggested that strains 01SU5-PT and 03SU3-PT represent two separate novel species in the genus Sphingopyxis. Several phenotypic characteristics served to differentiate these two isolates from recognized members of the genus Sphingopyxis. The data from the polyphasic study presented here indicated that strains 01SU5-PT and 03SU3-PT should be classified as representing novel species in the genus Sphingopyxis, for which the names Sphingopyxis rigui sp. nov. and Sphingopyxis wooponensis sp. nov., respectively, are proposed. The type strain of Sphingopyxis rigui sp. nov. is 01SU5-PT (= KCTC 23326T = JCM 17509T) and the type strain of Sphingopyxis wooponensis sp. nov. is 03SU3-PT (= KCTC 23340T = JCM 17547T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 07/2012; · 2.11 Impact Factor
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ABSTRACT: Thirty-seven carbofuran-degrading bacteria were isolated from agricultural soils, and their genetic and phenotypic characteristics were investigated. The isolates were able to utilize carbofuran as a sole source of carbon and energy. Analysis of the 16S rRNA gene sequence indicated that the isolates were related to members of the genera Rhodococcus, Sphingomonas, and Sphingobium, including new types of carbofuran-degrading bacteria, Bosea and Microbacterium. Among the 37 isolates, 15 different chromosomal DNA patterns were obtained by polymerase chain reaction (PCR) amplification of repetitive extragenic palindromic (REP) sequences. Five of the 15 representative isolates were able to degrade carbofuran phenol, fenoxycarb, and carbaryl, in addition to carbofuran. Ten of the 15 representative isolates had 1 to 8 plasmids. Among the 10 plasmid-containing isolates, plasmid-cured strains were obtained from 5 strains. The cured strains could not degrade carbofuran and other pesticides anymore, suggesting that the carbofuran degradative genes were on the plasmid DNAs in these strains. When analyzed with PCR amplification and dot-blot hybridization using the primers targeting for the previously reported carbofuran hydrolase gene (mcd), all of the isolates did not show any positive signals, suggesting that their carbofuran hydrolase genes had no significant sequence homology with the mcd gene.
Journal of Microbiology and Biotechnology 04/2012; 22(4):448-56. · 1.38 Impact Factor
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ABSTRACT: A non-motile, coccobacilli-shaped and pink pigmented bacterium, designated strain 03SU10-PT, was isolated from the wetland freshwater (Woopo wetland, Republic of Korea). Cells were Gram reaction-negative, catalase- positive and oxidase-negative. The major fatty acids (> 10% of total) were C18:1ω7c (59.6 %) and summed feature 3 (iso-C15:0 2-OH and/or C16:1ω7c) (13.7 %). The predominant respiratory lipoquinone was Q-10. The DNA G+C content was 68 mol%. The major polar lipids were phosphatidylethanoldiamine, phosphatidylcholine and unknown aminolipid. Spermidine, putrescine and 1,3-diaminopropane were the major polyamine. A phylogenetic tree based on 16S rRNA gene sequence comparison showed that strain 03SU10-PT formed an evolutionary lineage within the radiation enclosing the members of the genus Roseomonas and its nearest neighbor was Roseomonas stagni (96.3 % sequence similarity). Polyphasic evidence presented in this study indicated that strain 03SU10-PT could not be assigned to any recognized species; therefore a novel species was proposed, Roseomonas riguiloci sp. nov., with 03SU10-PT (=KCTC 23339T =JCM 17520T) as the type strain.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2012; · 2.11 Impact Factor
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ABSTRACT: We have carried out a polyphasic taxonomic characterization of Bacillus beijingensis DSM 19037(T) and Bacillus ginsengi DSM 19038(T), which are closely related phylogenetically to Bhargavaea cecembensis LMG 24411(T). All three strains are Gram-stain-positive, non-motile, moderately halotolerant and non-spore-forming. 16S rRNA gene sequence analyses showed that the strains constituted a coherent cluster, with sequence similarities between 99.7 and 98.7 %. The percentage similarity on the basis of amino acid sequences deduced from partial gyrB gene nucleotide sequences of these three type strains was 96.1-92.7 %. Phylogenetic trees based on the 16S rRNA gene and GyrB amino acid sequences, obtained by using three different algorithms, were consistent and showed that these three species constituted a deeply rooted cluster separated from the clades represented by the genera Bacillus, Planococcus, Planomicrobium, Sporosarcina, Lysinibacillus, Viridibacillus, Kurthia and Geobacillus, supporting their placement in the genus Bhargavaea. All three type strains have menaquinone MK-8 as the major respiratory quinone and showed similar fatty acid profiles. The main polar lipids present in the three type strains were diphosphatidylglycerol and phosphatidylglycerol, and the three strains showed peptidoglycan type A4α with l-lysine as the diagnostic diamino acid. The DNA G+C contents of Bacillus beijingensis DSM 19037(T), Bacillus ginsengi DSM 19038(T) and Bhargavaea cecembensis LMG 24411(T) were 53.1, 50.2 and 53.7 mol%, respectively. The level of DNA-DNA hybridization among the three strains was 57-39 %, indicating that they are members of different species of the genus Bhargavaea. The phenotypic data are consistent with the placement of these three species in a single genus and support their differentiation at the species level. On the basis of these data, we have emended the description of the genus Bhargavaea and propose the reclassification of Bacillus beijingensis and Bacillus ginsengi to the genus Bhargavaea, as Bhargavaea beijingensis comb. nov. (type strain ge10(T) = DSM 19037(T) = CGMCC 1.6762(T)) and Bhargavaea ginsengi comb. nov. (type strain ge14(T) = DSM 19038(T) = CGMCC 1.6763(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2011; 62(Pt 10):2495-504. · 2.11 Impact Factor
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ABSTRACT: A metagenomic fosmid library was constructed using a genomic DNA mixture extracted from the gut microflora of abalone. The library gave an alginate lyase positive clone (AlyDW) harboring a 31.7-kbp insert. The AlyDW insert consisted of 22 open reading frames (ORFs). The deduced amino acid sequences of ORFs 11-13 were similar to those of known alginate lyase genes, which are found adjacent in the genome of Klebsiella pneumoniae subsp. aerogenes, Vibrio splendidus, and Vibrio sp. belonging to the phylum Gammaproteobacteria. Among the three recombinant proteins expressed from the three ORFs, alginate lyase activity was only observed in the recombinant protein (AlyDW11) coded by ORF 11. The expressed protein (AlyDW11) had the highest alginate lyase activity at pH 7.0 and 45°C in the presence of 1 mM AgNO(3). The alginate lyase activity of ORF 11 was confirmed to be endolytic by thin-layer chromatography. AlyDW11 preferred poly(β-D: -mannuronate) as a substrate over poly(α-L: -guluronate). AlyDW11 contained three highly conserved regions, RSEL, QIH, and YFKAGVYNQ, which may act to stabilize the three-dimensional conformation and function of the alginate lyase.
Journal of Industrial Microbiology 11/2011; 39(4):585-93. · 1.80 Impact Factor
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ABSTRACT: An orange, rod-shaped, gliding bacterium, designated strain PSC33(T), was isolated from the gut microflora of a mussel collected from Gwangyang Bay, South Sea (Republic of Korea). Cells were Gram-reaction-negative, strictly aerobic, and catalase- and oxidase-positive. The major fatty acids were iso-C(15:0), iso-C(17:0) 3-OH, iso-C(15:1) G, C(15:0) 3-OH and iso-C(17:1)ω9c. The only isoprenoid quinone was menaquinone-6 (MK-6). The DNA G+C content of strain PSC33(T) was 37.9 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain PSC33(T) formed an evolutionary lineage within the radiation encompassing members of the genus Aquimarina with Aquimarina macrocephali JAMB N27(T) as its nearest neighbour (96.3% sequence similarity). A number of phenotypic characteristics distinguished strain PSC33(T) from recognized members of the genus Aquimarina. On the basis of the data presented in this study, strain PSC33(T) is considered to represent a novel species of the genus Aquimarina, for which the name Aquimarina mytili sp. nov. is proposed. The type strain is PSC33(T) ( = KCTC 23302(T) = JCM 17454(T)). An emended description of A. macrocephali is also provided.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2011; 62(Pt 8):1974-9. · 2.11 Impact Factor
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ABSTRACT: A Gram-negative, facultatively anaerobic, rod-shaped, catalase- and oxidase-positive bacterium, motile by means of a single polar flagellum and designated strain KJW27(T), was isolated from the marine sediment of Karwar jetty, west coast of India. The strain was β-haemolytic and grew with 0-10 % (w/v) NaCl, at 10-45 °C and at pH 6.5-10, with optimum growth with 2 % (w/v) NaCl, at 37 °C and at pH 7.5. The major fatty acids were iso-C₁₅:₀ (22.2 %), C₁₇:₁ω8c (21 %), summed feature 3 (comprising C₁₆:₁ω7c and/or C₁₆:₁ω6c; 10.2 %), C₁₆:₀ (7.1 %), iso-C₁₃:₀ (5.6 %) and C₁₇:₀ (4.4 %). The DNA G+C content was 51.2 mol%. Phylogenetic analysis based on 16S rRNA and gyrB gene sequences showed that strain KJW27(T) forms a lineage within the genus Shewanella and is closely related to Shewanella algae ATCC 51192(T) (98.8 %), Shewanella haliotis DW01(T) (98.8 %) and Shewanella chilikensis JC5(T) (98.2 %). Sequence identity with other members of this genus ranges from 92.2 to 96.4 %. The DNA-DNA relatedness of strain KJW27(T) with S. algae ATCC 51192(T), S. haliotis DW01(T) and S. chilikensis JC5(T) was 52, 44 and 33 %, respectively. The phenotypic, genotypic and DNA-DNA relatedness data indicate that strain KJW27(T) should be distinguished from S. algae ATCC 51192(T), S. haliotis DW01(T) and S. chilikensis JC5(T). On the basis of the data presented in this study, strain KJW27(T) represents a novel species, for which the name Shewanella indica sp. nov. is proposed. The type strain is KJW27(T) ( = KCTC 23171(T) = BCC 41031(T) = NCIM 5388(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 09/2011; 61(Pt 9):2058-64. · 2.11 Impact Factor
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ABSTRACT: A Gram-stain-positive, ovoid to short rod-shaped, yellow-pigmented bacterium, designated strain DY66(T), was isolated from tidal-flat sediment collected from Deukryang Bay (Republic of Korea), and its taxonomic position was investigated by using a polyphasic approach. Strain DY66(T) grew optimally at 30 °C and pH 8-9 and in 2 % (w/v) NaCl. The peptidoglycan type was A4α, L-Lys-L-Ala-D-Glu, and tyvelose and glucose were the major cell-wall sugars. The predominant menaquinones were MK-10 and MK-9. Major cellular fatty acids (>10 % of total) were anteiso-C(15 : 0) and iso-C(15 : 0). The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, an unknown aminophospholipid and three unknown aminolipids. The DNA G+C content was 59.1 mol%. This chemotaxonomic profile supported the assignment of strain DY66(T) to the genus Zhihengliuella. Phylogenetic analysis based on 16S rRNA gene sequences also indicated that strain DY66(T) belonged to the family Micrococcaceae and was related to the genus Zhihengliuella. On the basis of the evidence presented in this study, strain DY66(T) represents a novel species of the genus Zhihengliuella, for which the name Zhihengliuella aestuarii sp. nov. is proposed. The type strain is strain DY66(T) ( = KCTC 19557(T) = JCM 16364(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 07/2011; 61(Pt 7):1671-6. · 2.11 Impact Factor
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ABSTRACT: With the aim of isolating a biocatalyst able to catalyze biodiesel production from microbial source, Ralstonia sp. CS274 was isolated and a lipase from the strain (RL74) was purified. Molecular weight of RL74 was estimated to be 28,000 Da by SDS-PAGE. The activity was highest at 50-55°C and pH 8.0-9.5 and was stable at pH 7.0-12.0 and up to 45°C. It was resistant to oxidizing and reducing agents and the activity was enhanced by detergents. RL74 was 1,3 specific and K(m) and V(max) for p-nitrophenyl palmitate were 2.73 ± 0.6mM and 101.4 ± 1.9 mM/min mg, respectively. N-terminal amino acid sequence showed partial homology with that of Penicillium lipases. RL74 produced biodiesel more efficiently in palm oil than in soybean oil; and the production was highest at pH 8.0, at 5% methanol and at 20% water content.
Bioresource technology 02/2011; 102(10):6104-11. · 4.25 Impact Factor
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ABSTRACT: A rod-shaped, yellow and strictly aerobic marine bacterium, designated KYW382(T), was isolated from seawater collected from the South Sea, Republic of Korea. Cells were Gram-negative and catalase- and oxidase-positive. The major fatty acids were iso-C(15:1) G, iso-C(15:0), iso-C(17:0) 3-OH, iso-C(15:0) 3-OH and anteiso-C(15:0). The DNA G+C content was 32.4 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain KYW382(T) constituted an evolutionary lineage within the radiation enclosing the members of the genus Gaetbulibacter. The closest neighbour was Gaetbulibacter saemankumensis SMK-12(T) (96.1% 16S rRNA gene sequence similarity). A number of phenotypic characteristics distinguished strain KYW382(T) from the described members of the genus Gaetbulibacter. On the basis of the data presented in this study, strain KYW382(T) represents a novel species, for which the name Gaetbulibacter aestuarii sp. nov. is proposed. The type strain is KYW382(T) (=KCTC 23303(T) =JCM 17455(T)). An emended description of the genus Gaetbulibacter is also given.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2011; 62(Pt 1):150-4. · 2.11 Impact Factor
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ABSTRACT: A metagenomic fosmid library was constructed using genomic DNA isolated from abalone intestine. Screening of a library of 3,840 clones revealed a 36 kb insert of a cellulase positive clone (pAMHElO). A shotgun clone library was constructed using the positive clone (pAMHElO) and further screening of 3,840 shotgun clones with an approximately 5 kb insert size using a Congo red overlay revealed only one cellulase positive clone (pAMHL9). The pAMHL9 consisted of a 5,293-bp DNA sequence and three open reading frames (ORFs). Among the three ORFs, cellulase activity was only shown in the recombinant protein (CelAMll) coded by ORF3, which showed 100% identity with outer membrane protein A from Vibrio alginolyticus 12G01, but no significant sequence homology to known cellulases. The expressed protein (CelAMll) has a molecular weight of approximately 37 kDa and the highest CMC hydrolysis activity was observed at pH 7.0 and 37°C. The carboxymethyl cellulase activity was determined by zymogram active staining and different degraded product profiles for CelAMll were obtained when cellotetraose and cellopentaose were used as the substrates, while no substrate hydrolysis was observed on oligosaccharides such as cellobiose and cellotriose.
The Journal of Microbiology 02/2011; 49(1):141-5. · 1.10 Impact Factor
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ABSTRACT: A rod-shaped, endospore-forming, Gram-reaction-positive bacterium, designated strain WPCB018(T), was isolated from a fresh water sample collected from Woopo wetland, Korea. The isolate was identified as a member of the genus Paenibacillus on the basis of phenotypic characteristics and phylogenetic inference based on 16S rRNA gene sequence analysis. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and unknown aminophospholipids. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C(15 : 0) (32.2 %), C(16 : 0) (20.1 %) and C(18 : 0) (18.1 %). The DNA G+C content was 56.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain WPCB018(T) belongs to a cluster comprising species of the genus Paenibacillus, its closest neighbours being Paenibacillus humicus PC-147(T) (97.5 %) and Paenibcillus pasadenensis SAFN-007(T) (96.2 %). Genomic DNA-DNA hybridizations performed with strain WPCB018(T) and type strains of the species P. humicus, P. pinihumi, P. phyllosphaerae, P. pasadenensis and P. tarimensis showed relatedness values of only 10, 17, 18, 19 and 20 %, respectively. On the basis of phenotypic, molecular and genetic evidence, strain WPCB018(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus wooponensis sp. nov. is proposed. The type strain of the novel species is WPCB018(T) ( = KCTC 13280(T) = JCM 16350(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2011; 61(Pt 11):2763-8. · 2.11 Impact Factor
