Jin-Mi Jung

Massachusetts Institute of Technology, Cambridge, Massachusetts, United States

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Publications (16)98.29 Total impact

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    ABSTRACT: A major challenge facing drug delivery is efficient targeting of bioactive cargo to specific cells or tissues. Delivery of macromolecular cargo (e.g., nucleic acids, proteins) to cells for example, is hindered by the inability of these cargos to escape membrane bound intra-cellular compartments (endosomes) during cell delivery/uptake, resulting in cargo degradation. Developing new clinically-relevant treatments requires drug delivery systems that are both biocompatible and that overcome such cellular delivery barriers. Gold nanoparticles (AuNPs) have become of great interest in drug delivery because these materials can be functionalized with a range of biological cargos with minimal toxicity, and can be efficiently cleared from the body. Highly ordered self-assembled monolayers can be assembled on the surface of small gold particles, and mixtures of hydrophobic and hydrophilic organic ligands packed on the surfaces of AuNPs via chemisorption can self-organize into nanoscale “striped” surface morphologies that can be exploited to control the interactions of AuNPs with cells. We recently reported that highly water-soluble striped NPs that display amphiphilic mixed ligand shells composed of 11-mercapto-1-undecanesulphonateand 1-octanethiol are capable of penetrating the plasma membrane of cells, in contrast to AuNPs bearing the same hydrophilic/hydrophobic ligands in a disordered arrangement, which are taken up by endocytosis. Here we describe a new strategy for endocytosis-independent (i.e. cell penetrating) delivery of large, membrane-impermeable macromolecular cargo (e.g., oligonucleotides) using striped cell-penetrating AuNP carriers. To determine whether striped AuNPs would retain their cell entry properties when conjugated to large polar drug cargos, the cellular uptake of striped NPs (and non-striped control particles) conjugated with thiol-terminated oligonucleotides was assessed. Strikingly, striped AuNPs conjugated to both single-stranded and double-stranded oligonucleotides were taken up by cells in vitro, even under conditions where active uptake processes (i.e., endocytosis) were blocked by incubation at 4 degrees C or through the use of pharmacological inhibitors. Control sulfonate-capped particles lacking the striped domains failed to promote oligonucleotide uptake when endocytic processes were blocked in cells, and were taken up at 50% lower levels at 37 degrees C compared to striped particles. Confocal microscopy confirmed cystosolic localization of particle-conjugated oligonucleotides carried into cells by striped AuNPs. Total oligonucleotide uptake was inversely related to oligonucleotide length for single-stranded oligonucleotides attached to striped particles, and uptake of double-stranded oligonucleotides linked to striped particles was significant but did not vary as a function of oligonucleotide length. Together, these data suggest that striped AuNPs are of interest for intracellular delivery of membrane-impermeable drug cargos such as siRNA or immunostimulatory DNA, and ongoing studies are exploring the potential of these materials for modulating function in live cells.
    12 AIChE Annual Meeting; 11/2012
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    ACS Nano 04/2012; DOI:10.1021/nn301463w · 12.03 Impact Factor
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    ABSTRACT: We report on the fabrication of well-aligned multi-segment line patterns over large areas featuring dimensional and compositional exquisite tunability using a combination of photolithography and soft-lithography techniques. We show that thus this new top-down approach has great advantages and that it is beneficial by increasing the control of the multi-segment line width and pattern feature dimensions ranging from microns to a few hundred nanometres. Various combinations of multisegment materials with full control over the periodic alignment, which include Au–Ni, Au–Cu and Au–Ag, were prepared by simply changing the metals evaporated before the lift-off process. Au–Ni multi-segment metal line patterns showed a linear current–voltage response, identical with that of a line pattern from a single material. Thus, one can take advantage of the simple electrical properties of the 1-dimensional nanostructure. Our approach provides great potential in practical fabrication of well-integrated multi-metal component devices for electrical and optical detection.
  • Angewandte Chemie International Edition 12/2011; 50(51):12312-5. DOI:10.1002/anie.201104514 · 11.26 Impact Factor
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    ABSTRACT: Interfacial adhesion properties between steel and epoxy layers were improved considerably by fabricating periodic square-shaped dot patterns instead of random roughness via a combination of photolithography and wet chemical etching method. Because adhesion properties are determined generally by a roughness and topography of their surfaces, several approaches have been made to control the surface roughness, topography, and energy, for example, the surface grinding via sand papers, the surface etching by acid treatments, and the surface treatments by plasma gases. Here we confirm the enhanced adhesion performance by 40% via the periodic square-shaped dot patterns compared to conventional methods. Such remarkable enhancement in the adhesion of the dot patterned steel is due to the increased cohesive fracture area and topological interlocking. We believe that this approach is very simple and truly cost-effective and can be applicable to other microelectronics and mechanical systems and will provide potential opportunities for film/coating applications.
    Journal of Applied Physics 04/2011; 109(7). DOI:10.1063/1.3567113 · 2.19 Impact Factor
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    ABSTRACT: Silver has been widely used for optical sensing and imaging applications which benefit from localized surface plasmon resonance (LSPR) in a nanoscale configuration. Many attempts have been made to fabricate and control silver nanostructures in order to improve the high performance in sensing and other applications. However, a fatal mechanical weakness of silver and a lack of durability in oxygen-rich conditions have disrupted the manufacturing of reproducible nanostructures by the top-down lithography approach. In this study, we suggest a steady fabrication strategy to obtain highly ordered silver nanopatterns that are able to provide tunable LSPR characteristics. By using a protecting layer of platinum on a silver surface in the lithography process, we successfully obtained large-area (2.7 × 2.7 mm(2)) silver nanopatterns with high reproducibility. This large-area silver nanopattern was capable of enhancing the low concentration of a Cy3 fluorescence signal (∼10(-10) M) which was labeled with DNA oligomers.
    Nanotechnology 03/2011; 22(9):095304. DOI:10.1088/0957-4484/22/9/095304 · 3.67 Impact Factor
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    ABSTRACT: Nanotechnology as well as advanced microscopy can play a fundamental role in understanding biological mechanisms. Here we present a study that combines a new type of nanomaterial with a new type of microscopy and highlights the potential for gathering novel information about cell membrane penetration and cytosol local viscosity. On the material side, we used gold nanoparticles that have an ordered stripe-like arrangement of domains. These "striped" nanoparticles are able to penetrate cell membranes directly without porating them. On the microscopy side, we used photothermal heterodyne imaging which allows detection of individual nanometer-sized gold particles in complex media. We showed that we can probe cytosolic presence as well as dynamics of these nanoparticles even at very low concentrations. We used the fluctuations of the photothermal signal from particles diffusing in the detection volume to estimate local cytosol viscosity which is about 20 times larger than that of water. This work opens new perspectives for mapping local diffusion properties of nano-objects inside living cells.
    ACS Nano 03/2011; 5(4):2587-92. DOI:10.1021/nn1023285 · 12.03 Impact Factor
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    ABSTRACT: We have introduced patterned gold dots between the electrodes of single walled carbon nanotube (SWNT) network films, to effectively guide the path of randomly assembled nanotube networks. Direct visualization results show well-aligned SWNT networks across the gold dots, generating improved SWNT–FET performance with a high on/off ratio of 104 and 85 cm2 V−1 s−1 mobility, a remarkable enhancement compared to SWNT–FET in the absence of patterned gold dots. Unlike previously reported alignment techniques used, the proposed method affords the selective alignment of SWNTs within electrodes at room temperature without any direct growth on the device surface, and without the need for further alignment procedures. Thus, this gold-dot pattern technique within FET channel has proven to be truly simple, reliable and cost-effective. We discuss the effect of the voltage, channel size and feature dimensions of the gold nanopatterns in field effect transistor (FET) structures.
    Journal of Materials Chemistry 01/2011; 21(37):14285. DOI:10.1039/C1JM11241D · 7.44 Impact Factor
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    ABSTRACT: The variously shaped gold patterns can be generated from the polydimethylsiloxane (PDMS) mold using line patterns by the capillary force lithography (CFL) process, which is a kind of nanoimprint method following the two-cycle method. After fabrication of micro- or nanosized line patterns at the first cycle, the patterned substrate is used as a substrate for the second cycle of CFL. When the other stamp is placed on the first pattern, rotated by a certain angle with respect to the first stamp, only the overlapped parts remained dot-shaped after the etching process. The various shapes and sizes of patterns can be produced by controlling the CFL conditions such as polymer thickness, reactive ion etching (RIE) time, and degree of rotation angle. The key advantage of the double imprint lithography method is to get the nanosized isolated dot-shaped patterns from microsized line patterns. If we fabricate nanosized isolated dot-shaped patterns directly, we should need predesigned patterns in the form of a master, which is generally prepared by a high-cost and time-consuming process such as E-beam lithography. The successful applications of large-area periodic patterns are nanoelectronic devices, nanoelectromechanical system (NEMS), and biosensors, the template of which is the master of nanoimprint lithography (NIL) and stamp fabrication in soft lithography.
    Langmuir 09/2010; 26(17):14359-63. DOI:10.1021/la100414c · 4.46 Impact Factor
  • Advanced Materials 06/2010; 22(23):2542-6. DOI:10.1002/adma.200903745 · 15.41 Impact Factor
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    ABSTRACT: The rapid development of molecular biology is creating a pressing need for arrays of biomolecules that are able to detect smaller and smaller volumes of analytes. This goal can be achieved by shrinking the average size and spacing of the arrays' constituent features. While bioarrays with dot size and spacing on the nanometer scale have been successfully fabricated via scanning probe microscopy-based techniques, such fabrication methods are serial in nature and consequently slow and expensive. Additionally, the development of truly small arrays able to analyze scarce volumes of liquids is hindered by the present use of optical detection, which sets the minimum dot spacing on the order of roughly half the excitation wavelength. Here, we show that supramolecular nanostamping, a recently introduced truly parallel method for the stamping of DNA features, can efficiently reproduce DNA arrays with features as small as 14 +/- 2 nm spaced 77 +/- 10 nm. Moreover, we demonstrate that hybridization of these nanoarrays can be detected using atomic force microscopy in a simple and scaleable way that additionally does not require labeling of the DNA strands.
    Nano Letters 12/2007; 7(11):3493-8. DOI:10.1021/nl0720758 · 13.59 Impact Factor
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    ABSTRACT: In this paper, we examined the characteristic behavior of localized surface plasmon resonances (LSPR) of Au dot and ring arrays in response to the selective binding of biomolecules. To do this, patterned arrays of Au rings and dots with various feature scales were fabricated over large areas by an imprint lithography technique. Our results showed that the LSPR spectra of the Au nanorings exhibited a blue shift with increase in the ring widths and asymptotically converged to those for Au nanodots. This clearly implies that the LSPR spectra can be tuned over an extended wavelength range by varying the ring width. For an illustrative purpose, the patterned Au structures were used to detect the binding of streptavidin to biotin. In doing this, the Au patterns were chemically modified with G4 dendrimers of amine terminated poly(amidoamine), which facilitated the tethering of biotin onto the Au pattern. Exposure of the biotinylated Au nanorings to aqueous streptavidin solution induced both red-shifts of the LSPR spectra and changes in the peak intensities. The sensitivity of the LSPR spectra to the binding of the biomolecules was enhanced as the ring width of Au rings was decreased.
    Langmuir 09/2006; 22(17):7109-12. DOI:10.1021/la0605844 · 4.38 Impact Factor
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    ABSTRACT: A method to immobilize proteins at predefined position using gold-conjugated protein nanoarrays was investigated. Two different proteins, gold nanoparticle-conjugated streptavidin and gold-conjugated protein G molecules were selected as model proteins in the study. The individual protein molecules are immobilized and stabilized with inherent spacing without interfering with the intermolecular interactions. The electrochemical behavior of the exposed gold film was studied as a function of the RIE time to verify whether the ordered pore region is exposed to the underlying surface, which is critical for the immobilization of probe biomolecules. The selective deposition of SA-AuNPs on the biotin-modified surface was further supported by CV analysis, where the voltammetric peak current associated with the redox pair became substantially lower as the surface became blocked by adsorbed streptavidin molecule. This method provides a promising tool for biosensor applications.
    Small 08/2006; 2(8-9):1010-5. DOI:10.1002/smll.200600005 · 7.51 Impact Factor
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    ABSTRACT: We describe a method to improve the sensitivity of surface plasmon resonance (SPR) immunoassays using a horseradish peroxidase (HRP)-catalyzed precipitation reaction. The precipitation reaction catalyzed by HRP bound to the SPR biosensor surface via a sandwich immunoassay induced a shift in the SPR angle. Human interferon (IFN)-gamma at concentrations ranging from 0.01 to 100 ng/ml was detectable by this method. We also show that this biocatalytic signal amplification method can be applied to SPR imaging (SPRI), in an immunoassay of multiple proteins on a protein microarray format.
    Journal of Immunological Methods 03/2005; 297(1-2):125-32. DOI:10.1016/j.jim.2004.12.007 · 2.01 Impact Factor
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    ABSTRACT: A surface plasmon resonance (SPR) imaging system was constructed and used to detect the affinity-tagged recombinant proteins expressed in Escherichia coli. With regards to model proteins, the hexahistidine-ubiquitin-tagged human growth hormone (His(6)-Ub-hGH), glutathione S-transferase-tagged human interleukin-6 (GST-hIL6), and maltose-binding protein-tagged human interleukin-6 (MBP-hIL6) expressed in E. coli were analyzed. The cell lysates were spotted on gold thin films coated with 11-mercaptoundecanol (MUOH)/dextran derivatized with Ni(II)-iminodiacetic acid (IDA-Ni(II)), glutathione, or cyclodextrin. After a brief washing of the gold chip, SPR imaging measurements were carried out in order to detect the bound affinity-tagged fusion proteins. Using this new approach, rapid high-throughput expression analysis of the affinity-tagged proteins were obtained. The SPR imaging protein chip system used to measure the expression of affinity-tagged proteins in a high-throughput manner is expected to be an attractive alternative to traditional laborious and time-consuming methods, such as SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blots.
    Analytical Biochemistry 08/2004; 330(2):251-6. DOI:10.1016/j.ab.2004.02.009 · 2.31 Impact Factor
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    ABSTRACT: S The single molecule approach is potentially of great technological interest as a complementary means to obtain information about protein conformation or molecular interaction. The measurement for molecular interaction in single molecule level requires that the molecule in the question is immobilized by binding to an appropriate surface. W have developed a novel fabrication method of nano-patterned array of bio-molecules with ultra-high density, using self-assembly of block-copolymers. Compared to previous lithographic methods, self-assembly of block-copolymers makes it easier to fabrication of patterns with ~ 10 nanometer feature size and to reduce the number of processing steps. In this work, we have detected a single molecule array on the basis of immunoreaction using protein conjugated gold nanoparticle. In this manner, BCP structure played an important role as confining molecules in single molecule level to a hole avoiding intermolecular interaction or nonspecific binding.

Publication Stats

298 Citations
98.29 Total Impact Points


  • 2010–2012
    • Massachusetts Institute of Technology
      • Department of Materials Science and Engineering
      Cambridge, Massachusetts, United States
  • 2006–2012
    • Korea Advanced Institute of Science and Technology
      • Department of Chemical and Biomolecular Engineering
      Sŏul, Seoul, South Korea
  • 2004–2005
    • Korea Research Institute of Bioscience and Biotechnology KRIBB
      • BioNanotechnology Research Center
      Anzan, Gyeonggi-do, South Korea