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Nathalie Pradel,
Boyang Ji,
Grégory Gimenez,
Emmanuel Talla,
Patricia Lenoble,
Marc Garel,
Christian Tamburini,
Patrick Fourquet,
Régine Lebrun,
Philippe Bertin,
Yann Denis,
Matthieu Pophillat,
Valérie Barbe, Bernard Ollivier,
Alain Dolla
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ABSTRACT: Desulfovibrio piezophilus strain C1TLV30(T) is a piezophilic anaerobe that was isolated from wood falls in the Mediterranean deep-sea. D. piezophilus represents a unique model for studying the adaptation of sulfate-reducing bacteria to hydrostatic pressure. Here, we report the 3.6 Mbp genome sequence of this piezophilic bacterium. An analysis of the genome revealed the presence of seven genomic islands as well as gene clusters that are most likely linked to life at a high hydrostatic pressure. Comparative genomics and differential proteomics identified the transport of solutes and amino acids as well as amino acid metabolism as major cellular processes for the adaptation of this bacterium to hydrostatic pressure. In addition, the proteome profiles showed that the abundance of key enzymes that are involved in sulfate reduction was dependent on hydrostatic pressure. A comparative analysis of orthologs from the non-piezophilic marine bacterium D. salexigens and D. piezophilus identified aspartic acid, glutamic acid, lysine, asparagine, serine and tyrosine as the amino acids preferentially replaced by arginine, histidine, alanine and threonine in the piezophilic strain. This work reveals the adaptation strategies developed by a sulfate reducer to a deep-sea lifestyle.
PLoS ONE 02/2013; 8(1):e55130. · 4.09 Impact Factor
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ABSTRACT: A novel obligately anaerobic, non-spore-forming, rod-shaped mesophilic, halophilic bacterium staining Gram-negative, was isolated from sediments of Guaymas basin. The strain, designated Ra1766G1T, grew at 20-40 °C (optimum 30-35 °C) and at pH 6.0-8.0 (optimum pH 6.5-7.5). It required 0.5%-7.5% NaCl (optimum 2%-3%) for growth. Sulfate, thiosulfate, elemental sulfur, sulfite, fumarate, nitrate and nitrite were not used as terminal electron acceptors. Strain Ra1766G1T used cellobiose, glucose, mannose, maltose, arabinose, raffinose, galactose, ribose, saccharose, pyruvate and xylose as electron donors. The main fermentation product from glucose metabolism was acetate. The predominant cellular fatty acids were anteiso-C15:0, iso-C15:0, anteiso DMA-C15:0 and C16:0. The main polar lipids consisted of diphosphatiglycerol, phosphatidylglycerol, glycolipids and phospholipids.The G+C content of the genomic DNA was 31.2 mol%. The closest phylogenetic relatives of Ra1766G1T were Natranaerovirga pectinivoraT (92.4% similarity), Natranaerovirga hydrolytica (90.2% similarity) and Defluviitalea saccharophilaT (88.9% similarity). On the basis of phylogenetic inference and phenotypic properties, strain Ra1766G1T (= DSM 24848T = JCMT= 16313) is proposed as the type strain of a novel species of a novel genus, Vallitalea guaymasensis gen. nov., sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2013; · 2.11 Impact Factor
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Wajdi Ben Hania,
Anne Postec,
Thomas Aüllo,
Anthony Ranchou-Peyruse,
Gael Erauso,
Céline Brochier-Armanet,
Moktar Hamdi, Bernard Ollivier,
Stéphanie Saint-Laurent,
Michel Magot,
Marie-Laure Fardeau
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ABSTRACT: Strain VNs100T, a novel mesophilic anaerobic rod-cocoid-shaped bacterium, having a sheath-like outer structure (toga) was isolated from a water sample collected in the area of underground gas storage. It was non-motile with cells appearing singly (2-4 μm long x 1-2 μm wide), in pairs, or as long chains and stained Gram-negative. Strain VNs100T was heterotrophic, able to use arabinose, cellobiose, fructose, galactose, glucose, lactose, lactate, mannose, maltose, raffinose, ribose, sucrose and xylose as energy sources only in the presence of elemental sulfur as terminal electron acceptor. Acetate, CO2 and sulfide were the end-products of sugar metabolism. Hydrogen was not detected. Elemental sulfur, but not thiosulfate, sulfate and sulfite, were reduced into sulfide. It grew at temperatures between 30°C and 50°C (optimum 45°C), at pH between 6.2 and 7.9 (optimum 7.3-7.5) and at NaCl concentrations between 0 and 15 g.L-1 (optimum 2 g.L-1). The DNA G+C content was 47.5 mol%. The main cellular fatty acid was C16:0. Phylogenetic analysis of the small-subunit (SSU) ribosomal RNA (rRNA) gene sequence indicated that strain VNs100T had as its closest relatives 'Mesotoga sulfurireducens' (97.1 % similarity) and Mesotoga prima (similarity of 97.1 % and 97.7 % with each of its two genes respectively) within the order Thermotogales. Hybridization between strain VNS100T and 'Mesotoga sulfurireducens' and between strain VNS100T and Mesotoga prima is 12.9% and 20.6 %, respectively. Based on phenotypic, phylogenetic and taxonomic characteristics, strain VNs100T is proposed as a novel species of genus Mesotoga within the family Thermotogaceae, order Thermotogales. The name Mesotoga infera, sp. nov. is proposed. The type strain is VNs100T (= DSM 25546 = JCM 18154).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2013; · 2.11 Impact Factor
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Boyang Ji,
Gregory Gimenez,
Valérie Barbe,
Benoît Vacherie,
Zoé Rouy,
Amira Amrani,
Marie-Laure Fardeau,
Philippe Bertin,
Didier Alazard,
Sabine Leroy,
Emmanuel Talla, Bernard Ollivier,
Alain Dolla,
Nathalie Pradel
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ABSTRACT: AM13 is a piezophilic, mesophilic, hydrogenotrophic sulfate-reducing bacterium collected from a deep-sea hydrothermal chimney on the East Pacific Rise (2,600 m depth, 13°N). We report the genome sequence of this bacterium, which includes a 3,702,934-bp chromosome and a circular plasmid of 5,328 bp.
Genome announcements. 01/2013; 1(1).
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ABSTRACT: Here, we report the draft genome sequence of Desulfotomaculum hydrothermale, a sulfate-reducing, spore-forming bacterium isolated from a Tunisian hot spring. The genome is composed of 2.7 Mb, with a G+C content of 49.48%, and it contains 2,643 protein-coding sequences.
Genome announcements. 01/2013; 1(1).
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ABSTRACT: A novel anaerobic thermophilic sulfate-reducing bacterium designated LINDBHT1 was isolated from an anaerobic treating abattoir wastewaters digestor in Tunisia. Strain LINDBHT1 grew at temperatures between 50 and 65°C (optimum 55-60°C), and at pH between 5.9 and 9.2 (optimum pH 6.0-6.8). Strain LINDBHT1 required salt for growth (1-40 g NaCl l-1), with an optimum at 20-30g/L. In the presence of sulfate as terminal electron acceptor, strain LINDBHT1 used H2/CO2, propanol, butanol and ethanol as carbon and energy sources but fumarate, formate, lactate and pyruvate were not utilized. Butanol was converted to butyrate, while propanol and ethanol were oxidized to propionate and acetate, respectively. Sulfate, sulfite and thiosulfate were utilized as terminal electron acceptors but elemental sulfur, iron (III), fumarate, nitrate and nitrite were not used. The G+C content of the genomic DNA was 44.4 mol %. Phylogenetic analysis of the small-subunit (SSU) ribosomal RNA (rRNA) gene sequence indicated that strain LINDBHT1 was affiliated to the genus Desulfotomaculum with Desulfotomaculum halophilum and Desulfotomaculum alkaliphilum as its closest phylogenetic relatives (about 89% of similarity). This bacterium will constitute a new species of the genus Desulfotomaculum, Desulfotomaculum peckii (the type strain LINDBHT1T = DSM 23769T = JCM 17209T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2012; · 2.11 Impact Factor
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ABSTRACT: A hyperthermophilic anaerobic bacterium, designated D2C22T was isolated from the hydrothermal hot spring of Guelma in the Northeast Algeria. The strain was Gram-positive, non-sporulated, not motile and rod-shaped, appearing singly or in pairs (0.3-0.4 µm x 8-9 µm). It grew anaerobically at temperatures between 50 and 80 °C (optimum 65 °C) and at pH between 5-9 (optimum 6.8). It did not require NaCl for growth, but tolerated it until 20 gl-1. Strain D2C22T used glucose, galactose, lactose, fructose, ribose, xylose, arabinose, maltose, cellobiose, mannose, melibiose, saccharose, xylan and pyruvate (only in the presence of yeast extract or biotrypcase) as electron donors. The end-products from glucose fermentation were acetate, lactate, CO2, and H2. Nitrate, nitrite, thiosulfate, elemental sulfur, sulfate, and sulfite were not used as electron acceptors. The predominant cellular fatty acids were iso-C15:0 and iso-C17:0. The DNA G+C content was 41.6 mol %. Phylogenetic analysis of the small-subunit (SSU) ribosomal RNA (rRNA) gene sequence indicated that strain D2C22T had Caldicoprobacter oshimai, Caldicoprobacter algeriensis and Acetomicrobium faecale as its closest relative (similarity of 95.5%, 95.5 % and 95.3 % respectively). Based on phenotypic, phylogenetic and taxonomic characteristics, strain D2C22T is proposed as a novel species of the genus Caldicoprobacter within the order Clostridiales, for which the name Caldicoprobacter guelmensis is proposed. The type strain is D2C22T (= DSM =24605T = JCM= 17646T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2012; · 2.11 Impact Factor
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ABSTRACT: A strictly anaerobic, moderately thermophilic, halotolerant, rod-shaped bacterium, was isolated from a water sample of a Tunisian hot spring. The strain designated BELH25T was non motile with cells appearing singly or in pairs (0.4-0.6 µm x 2-6 µm). It grew at temperatures between 45°C and 70°C (optimum 55°C), pH between 6.2 and 8.0 (optimum 7.0) and NaCl concentration between 0 and 4 % (optimum 0-2.0 %). Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Strain BELH25T used cellobiose, fructose, galactose, glucose, maltose, mannose, saccharose, starch and yeast extract as electron donors. The main fermentation products from glucose metabolism were formate, acetate, ethanol and CO(2). The predominant cellular fatty acids were iso- C15:0, iso- C17:0 and anteiso-C15:0. The DNA G+C content was 37.2 mol %. Phylogenetic analysis of the small-subunit (SSU) ribosomal RNA (rRNA) gene sequence indicated that strain BELH25T had Caloramator viterbiensis and Fervidicella metallireducens as its closest relatives (identity of 92.2 and 92.1 % respectively) and that the strain was positioned approximatively equidistantly between the two genera. Based on phenotypic, phylogenetic and taxonomic characteristics, strain BELH25T is proposed as a novel species of a new genus within the order Clostridiales, family Clostridiaceae for which the name Fonticella tunisiensis is proposed. The type strain is BELH25T (= DSM 24455 T = JCM 17559T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 09/2012; · 2.11 Impact Factor
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ABSTRACT: A thermophilic anaerobic bacterium (strain TH7C1T) was isolated from the hydrothermal hot spring of Guelma in the northeast of Algeria. Strain TH7C1T stained Gram-positive, was a non-motile rod appearing singly, in pairs, or as long chains (0.7–1×2–6μm2). Spores were never observed. It grew at temperatures between 55 and 75°C (optimum 65°C) and at pH between 6.2 and 8.3 (optimum
6.9). It did not require NaCl for growth, but tolerated it up to 5gl−1. Strain TH7C1T is an obligatory heterotroph fermenting sugars including glucose, galactose, lactose, raffinose, fructose, ribose, xylose,
arabinose, maltose, mannitol, cellobiose, mannose, melibiose, saccharose, but also xylan, and pyruvate. Fermentation of sugars
only occurred in the presence of yeast extract (0.1%). The end-products from glucose fermentation were acetate, lactate, ethanol,
CO2, and H2. Nitrate, nitrite, thiosulfate, elemental sulfur, sulfate, and sulfite were not used as electron acceptors. The G+C content
of the genomic DNA was 44.7mol% (HPLC techniques). Phylogenetic analysis of the small-subunit ribosomal RNA (rRNA) gene sequence
indicated that strain TH7C1T was affiliated to Firmicutes, order Clostridiales, family Caldicoprobacteraceae, with Caldicoprobacter
oshimai (98.5%) being its closest relative. Based on phenotypic, phylogenetic, and genetic characteristics, strain TH7C1T is proposed as a novel species of genus Caldicoprobacter, Caldicoprobacter algeriensis, sp. nov. (strain TH7C1T=DSM 22661T=JCM 16184T).
Current Microbiology 04/2012; 62(3):826-832. · 1.82 Impact Factor
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ABSTRACT: An obligately anaerobic, spore-forming, acidophilic sulfate-reducing bacterium, strain SJ4T, was isolated from an acid mining effluent decantation pond sediment sample (pH around 3.0). Cells were Gram negative, non-motile,
curved rods occurring singly. Strain SJ4T grew at pH 3.6–5.5 with an optimum at pH 5.2. Strain SJ4T utilized H2, lactate, pyruvate, glycerol, glucose, and fructose as electron donors. Lactate and glucose were weakly used. Sulfate was
used as electron acceptors, but not sulfite, elemental sulfur, arsenate (V), and fumarate. The G+C content of genomic DNA
was 42.3mol% (HPLC). 16S rRNA gene sequence analysis indicated that strain SJ4T belonged to the genus Desulfosporosinus within the family Peptococcaceae in the phylum Firmicutes. The level of 16S rRNA gene sequence similarity with other Desulfosporosinus species was 94.7–96.2%, D. orientis DSM 765T (similarity of 96.2%) and D. auripigmenti DSM 13351T (similarity of 95%) being its closest relatives. DNA–DNA relatedness values with D. orientis and D. auripigmenti were 16.5 and 31.8%, respectively. On the basis of phenotypic, phylogenetic, and genetic characteristics, strain SJ4T represents a novel species within the genus Desulfosporosinus, for which the name Desulfosporosinus acidiphilus sp. nov. is proposed. The type strain is SJ4T (=DSM 22704T=JCM 16185T).
KeywordsAcid mining drainage-Acidophilic-Sulfate reduction
Extremophiles 04/2012; 14(3):305-312. · 2.94 Impact Factor
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Linda Jabari,
Hana Gannoun,
Jean-Luc Cayol,
Abdeljabbar Hedi,
Mitsuo Sakamoto,
Enevold Falsen,
Moriya Ohkuma,
Moktar Hamdi,
Guy Fauque, Bernard Ollivier,
Marie-Laure Fardeau
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ABSTRACT: A novel obligately anaerobic, non-spore-forming, rod-shaped mesophilic bacterium, which stained Gram-positive but showed the typical cell wall structure of Gram-negative bacteria, was isolated from an upflow anaerobic filter treating abattoir wastewaters in Tunisia. The strain, designated LIND7H(T), grew at 20-45 °C (optimum 35-40 °C) and at pH 5.0-8.5 (optimum pH 6.5-7.5). It did not require NaCl for growth, but was able to grow in the presence of up to 2 % NaCl. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Strain LIND7H(T) used cellobiose, glucose, lactose, mannose, maltose, peptone, rhamnose, raffinose, sucrose and xylose as electron donors. The main fermentation products from glucose metabolism were lactate, acetate, butyrate and isobutyrate. The predominant cellular fatty acids were anteiso-C(15 : 0), C(15 : 0), C(17 : 0) 2-OH and a summed feature consisting of C(18 : 2)ω6,9c and/or anteiso-C(18 : 0), and the major menaquinones were MK-9, MK-9(H(2)) and MK-10. The G+C content of the genomic DNA was 41.4 mol%. Although the closest phylogenetic relatives of strain LIND7H(T) were Parabacteroides merdae, Parabacteroides goldsteinii and Parabacteroides gordonii, analysis of the hsp60 gene sequence showed that strain LIND7H(T) was not a member of the genus Parabacteroides. On the basis of phylogenetic inference and phenotypic properties, strain LIND7H(T) ( = CCUG 60892(T) = DSM 23697(T) = JCM 16313(T)) is proposed as the type strain of a novel species in a new genus within the family Porphyromonadaceae, Macellibacteroides fermentans gen. nov., sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2011; 62(Pt 10):2522-7. · 2.11 Impact Factor
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ABSTRACT: A novel strictly anaerobic bacterium designated SPDX02-08(T) was isolated from a deep terrestrial geothermal spring located in southwest France. Cells (1-2 × 2-6 μm) were non-motile, non sporulating and stained Gram negative. Strain SPDX02-08(T) grew at a temperature between 40 and 60°C (optimum 55°C), pH between 6.3 and 7.3 (optimum 7.2) and a NaCl concentration between 0 and 5 g/l (optimum 2 g/l). Sulfate, thiosulfate and sulfite were used as terminal electron acceptors, but not elemental sulfur, nitrate, nitrite, Fe (III) or fumarate. In the presence of sulfate, strain SPDX02-08(T) completely oxidized pyruvate, propionate, butyrate, isobutyrate, valerate, isovalerate and hexadecanoate. Stoichiometric measurements revealed a complete oxidation of part of lactate (0.125 mol of acetate produced per mole lactate oxidized). Strain SPDX02-08(T) required yeast extract to oxidize formate and H(2) but did not grow autotrophically on H(2). Among the substrates tested, only pyruvate was fermented. The G+C content of the genomic DNA was 57.6 mol%. Major cellular fatty acids of strain SPDX02-08(T) were iso-C(15:0), C(15:0), and C(16:0). Phylogenetic analysis of the 16S small-subunit (SSU) ribosomal RNA gene sequence indicated that strain SPDX02-08(T) belongs to the genus Desulfosoma, family Syntrophobacteraceae, having Desulfosoma caldarium as its closest phylogenetic relative (97.6% similarity). The mean DNA/DNA reassociation value between strain SPDX02-08(T) and Desulfosoma caldarium was 16.9 ± 2.7%. Based on the polyphasic differences, strain SPDX02-08(T) is proposed to be assigned as a new species of the genus Desulfosoma, Desulfosoma profundi sp. nov. (DSM 22937(T) = JCM 16410(T)). GenBank accession number for the 16S rRNA gene sequence of strain SPDX02-08(T) is HM056226.
Antonie van Leeuwenhoek 11/2011; 101(3):595-602. · 2.09 Impact Factor
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ABSTRACT: Strain SulfLac1(T), a thermophilic, anaerobic and slightly halophilic, rod-shaped bacterium with a sheath-like outer structure (toga), was isolated from a whey digester in Tunisia. The strain's non-motile cells measured 3-30×1 µm and appeared singly, in pairs or as long chains. The novel strain reduced thiosulfate and elemental sulfur, but not sulfate or sulfite, into sulfide. It grew at 37-65 °C (optimum 55 °C), at pH 6.5-7.9 (optimum pH 6.9) and with 0.2-3 % (w/v) NaCl (optimum 0.5 %). The G+C content of the strain's genomic DNA was 33.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SulfLac1(T) was most closely related to Petrotoga mobilis (91.4 % sequence similarity). Based on phenotypic, phylogenetic and chemotaxonomic evidence, strain SulfLac1(T) represents a novel species of a new genus within the order Thermotogales, for which the name Defluviitoga tunisiensis gen. nov., sp. nov. is proposed. The type strain of the type species is SulfLac1(T) ( = DSM 23805(T) = JCM 17210(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 08/2011; 62(Pt 6):1377-82. · 2.11 Impact Factor
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ABSTRACT: Strain BELH1(T), a novel mesophilic, anaerobic, halotolerant, rod-shaped bacterium, was isolated from a Tunisian wastewater digester. The cells of the strain are motile, measure 0.5×2-5 µm, and occur singly or in pairs. The strain reduced thiosulfate and elemental sulfur (but not sulfate or sulfite) into sulfide. It grew at 15-40 °C (optimum 30 °C), pH 5.8-8.4 (optimum 7) and with 0-10 % (w/v) NaCl (optimum 3.0 %). The genomic DNA G+C content of strain BELH1(T) was 38.2 mol% and the strain's predominant cellular fatty acids were C(14:0), a summed feature that contained iso-C(17:1) and/or anteiso-C(17:1) B, and C(16:0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain was most closely related to Fusibacter paucivorans (94.8 % sequence similarity). Based on phenotypic, phylogenetic and taxonomic characteristics, strain BELH1(T) represents a novel species of the genus Fusibacter, for which the name Fusibacter tunisiensis sp. nov. is proposed. The type strain is BELH1(T) ( = DSM 24436(T) = JCM 17481(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 08/2011; 62(Pt 6):1365-8. · 2.11 Impact Factor
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ABSTRACT: Magnetotactic bacteria (MTB) mineralize nanosized magnetite or greigite crystals within cells and thus play an important role in the biogeochemical process. Despite decades of research, knowledge of MTB distribution and ecology, notably in areas subjected to oil industry activities, is still limited. In the present study, we investigated the presence of MTB in the Gulf of Fos, French Mediterranean coast, which is subjected to intensive oil industry activities. Microcosms containing sediments/water (1:2, v/v) from several sampling sites were monitored over several weeks. The presence of MTB was revealed in five of eight sites. Diverse and numerous MTB were revealed particularly from one site (named CAR), whilst temporal variations of a homogenous magnetotactic cocci population was shown within the LAV site microcosm over a 4-month period. Phylogenetic analysis revealed that they belonged to Alphaproteobacteria, and a novel genus from the LAV site was evidenced. Among the physicochemical parameters measured, a correlation was shown between the variation of MTB abundance in microcosms and the redox state of sulphur compounds.
Microbial Ecology 07/2011; 63(1):1-11. · 2.91 Impact Factor
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Patrick Grégoire,
Malek Bohli,
Jean-Luc Cayol,
Manon Joseph,
Sophie Guasco,
Karine Dubourg,
Jean Cambar,
Valérie Michotey,
Patricia Bonin,
Marie-Laure Fardeau, Bernard Ollivier
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ABSTRACT: An anaerobic, thermophilic, filamentous (0.45 × >100 µm) bacterium, designated D1-25-10-4(T), was isolated from a deep hot aquifer in France. Cells were non-motile and Gram-negative. Growth was observed at 43-65 °C (optimum 55 °C), at pH 6.8-7.8 (optimum pH 7.0) and with 0-5 g NaCl l(-1) (optimum 0 g NaCl l(-1)). Strain D1-25-10-4(T) was a chemo-organotroph and fermented ribose, maltose, glucose, galactose, arabinose, fructose, mannose, sucrose, raffinose, xylose, glycerol, fumarate, peptone, starch and xylan. Yeast extract was required for growth. Sulfate, thiosulfate, sulfite, elemental sulfur, nitrate, nitrite and fumarate were not used as terminal electron acceptors. The G+C content of the DNA was 61.9 mol%. The major cellular fatty acids of strain D1-25-10-4(T) were C(17 : 0), C(18 : 0,) C(16 : 0) and iso-C(17 : 0). The closest phylogenetic relative of strain D1-25-10-4(T) was Caldilinea aerophila STL-6-O1(T) (97.9 % 16S rRNA gene sequence similarity). DNA-DNA relatedness between strain D1-25-10-4(T) and Caldilinea aerophila DSM 14535(T) was 8.7 ± 1 %. On the basis of phylogenetic, genotypic and phenotypic characteristics, strain D1-25-10-4(T) represents a novel species within the genus Caldilinea, class Caldilineae, phylum Chloroflexi, for which the name Caldilinea tarbellica sp. nov. is proposed. The type strain is D1-25-10-4(T) ( = DSM 22659(T) = JCM 16120(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2011; 61(Pt 6):1436-41. · 2.11 Impact Factor
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ABSTRACT: A new strictly anaerobic thermophilic multicellular filamentous bacterium (0.2-0.3μm×>100μm), designated GNS-1(T), was isolated from a deep hot aquifer in France. It was non-motile, and stained Gram-negative. Optimal growth was observed at 65°C, pH 7.0, and 2gL(-1) of NaCl. Strain GNS-1(T) was chemoorganotrophic fermenting ribose, glucose, galactose, arabinose, fructose, mannose, maltose, sucrose, xylose, raffinose, pyruvate, and xylan. Yeast extract was required for growth. The end products of glucose fermentation were lactate, acetate, CO(2), and H(2). The G+C content of the DNA was 57.6mol%. Its closest phylogenetic relative was Bellilinea caldifistulae with 92.5% similarity. Based on phylogenetic, genotypic and phenotypic characteristics, strain GNS-1(T) (DSM 23592(T), JCM 16980(T)) is proposed to be assigned to a novel species of a novel genus within the class Anaerolineae (subphylum I), phylum "Chloroflexi", Thermanaerothrix daxensis gen. nov., sp. nov. The GenBank accession number is HM596746.
Systematic and Applied Microbiology 05/2011; 34(7):494-7. · 3.37 Impact Factor
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ABSTRACT: Cultivated members of the order Thermotogales comprise only thermophilic to hyperthermophilic anaerobic microorganisms. However, based on molecular studies, the existence of mesophilic members ("mesotoga") within this order has been postulated but has not been demonstrated by cultural approaches so far. A "mesotoga" (strain PhosAc3) that belonged to an uncultivated lineage distantly related to the thermophilic Kosmotoga genus has now been cultivated in axenic culture. It grew between 30°C and 50°C (optimum 40°C) and oxidized lactate using elemental sulphur as a terminal electron acceptor. Further genomic and physiological characterization of strain PhosAc3 will be important not only for understanding bacterial adaptation to high and moderate temperatures at small evolutionary scales, but also because "mesotoga" might play a crucial ecological role in ecosystems polluted by aromatic compounds.
Systematic and Applied Microbiology 05/2011; 34(8):581-5. · 3.37 Impact Factor
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ABSTRACT: A novel thermophilic, anaerobic, Gram-stain-positive, terminal-spore-forming bacterium was isolated from an upflow anaerobic filter treating abattoir wastewaters in Tunisia. This strain, designated LIND6LT2(T), grew at 40-60 °C (optimum 50-55 °C) and at pH 6.0-8.5 (optimum pH 7.0-7.5). It did not require NaCl for growth, but tolerated it up to 2%. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as electron acceptors. Growth of LIND6LT2(T) was inhibited by sulfite (2 mM). Strain LIND6LT2(T) used cellobiose, glucose, mannose, maltose, mannitol, sucrose and xylose as electron donors. The main fermentation products from glucose metabolism were acetate, formate, butyrate and isobutyrate. The predominant cellular fatty acids were C(16:0) (68.4%) and C(14:0) (8.3%). The G+C content of the genomic DNA was 35.2 mol%. On the basis of its phylogenetic and physiological properties, a new genus and species, Defluviitalea saccharophila gen. nov., sp. nov., are proposed to accommodate strain LIND6LT2(T), placed in Defluviitaleaceae fam. nov. within the phylum Firmicutes, class Clostridia, order Clostridiales. Strain LIND6LT2(T) (=DSM 22681(T) =JCM 16312(T)) is the type strain of Defluviitalea saccharophila, which itself is the type species of Defluviitalea.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2011; 62(Pt 3):550-5. · 2.11 Impact Factor
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ABSTRACT: The hyperthermophile Thermotoga maritima, although strictly anaerobic, is able to grow in the presence of low amounts of O(2). Here, we show that this bacterium consumes O(2) via a three-partner chain involving an NADH oxidoreductase (NRO), a rubredoxin (Rd) and a flavo-diiron protein (FprA) (locus tags: TM_0754, TM_0659 and TM_0755, respectively). In vitro experiments showed that the NADH-dependent O(2) consumption rate was 881.9 (± 106.7) mol O(2) consumed min(-1) per mol of FprA at 37°C and that water was the main end-product of the reaction. We propose that this O(2) reduction chain plays a central role in the O(2) tolerance of T. maritima. Phylogenetic analyses suggest that the genes coding for these three components were acquired by an ancestor of Thermotogales from an ancestor of Thermococcales via a single gene transfer. This event likely also involved two ROS scavenging enzymes (neelaredoxin and rubrerythrin) that are encoded by genes clustered with those coding for FprA, NRO and Rd in the ancestor of Thermococcales. Such genomic organization would have provided the ancestor of Thermotogales with a complete set of enzymes dedicated to O(2)-toxicity defence. Beside Thermotogales and Thermococcales, horizontal gene transfers have played a major role in disseminating these enzymes within the hyperthermophilic anaerobic prokaryotic communities, allowing them to cope with fluctuating oxidative conditions that exist in situ.
Environmental Microbiology 03/2011; 13(8):2132-45. · 5.84 Impact Factor
