Publications (19)28.33 Total impact
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Article: A multicenter, open, noncomparative, phase II study of the combination of cladribine (2-chlorodeoxyadenosine), cytarabine, granulocyte colony-stimulating factor and mitoxantrone as induction therapy in refractory acute myeloid leukemia: a report of the Polish Adult Leukemia Group.
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ABSTRACT: Purine nucleoside analogues, cladribine (2-chlorodeoxyadenosine, 2-CdA) and fludarabine (FAMP) are active agents in acute myeloid leukemias (AMLs). Synergistic interaction between FAMP or 2-CdA with cytarabine (cytosine arabinoside, Ara-C) has been demonstrated in preclinical and clinical studies. The current multicenter phase II study was initiated to evaluate the efficacy and toxicity of induction treatment consisting of 2-CdA (5 mg/m2), Ara-C (2 g/m2), mitoxantrone (MIT, 10 mg/m2) and granulocyte colony-stimulating factor (G-CSF) (CLAG-M) in refractory AML. In case of partial remission, a second CLAG-M was administered. Patients in complete remission (CR) received consolidation courses based on high-dose Ara-C and MIT with or without 2-CdA. Forty-three patients from five centers were registered: 25 primary resistant and 18 relapsed. CR was achieved in 21 (49%) patients, 20 (47%) were refractory and 2 (5%) died early. Hematologic toxicity was the most prominent toxicity of this regimen. The overall survival (OS; 1 year) for the 42 patients as a whole and the 20 patients in CR were 43% and 73%, respectively. Disease-free survival (1 year) was 68.6%. None of the analyzed prognostic factors influenced the CR and OS probability significantly. We conclude that CLAG-M regimen has significant antileukemia activity in refractory AML, which seems to be better than the activity of many other regimens. The toxicity of the treatment is acceptable.Annals of Hematology 10/2005; 84(9):557-64. · 2.62 Impact Factor -
Article: Addition of cladribine to daunorubicin and cytarabine increases complete remission rate after a single course of induction treatment in acute myeloid leukemia. Multicenter, phase III study.
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ABSTRACT: To assess the efficacy of an original DAC-7 regimen: daunorubicine (DNR) 60 mg/m2/day, days 1-3; cytarabine (AraC) 200 mg/m2/day, days 1-7; cladribine (2-CdA) 5 mg/m2/day, days 1-5, 400 untreated adult acute myeloid leukemia patients (including 63 with preceding myelodysplastic syndrome), aged 45 (16-60) years were randomized to either DAC-7 (n=200) or DA-7 (without 2-CdA, n=200). The overall CR rate equaled 72% for DAC-7 and 69% for DA-7 arm (P=NS). After a single course of DAC-7 induction, the CR rate equaled 64% and was significantly higher compared to 47% in the DA-7 arm (P=0.0009). Median hospitalization time during the induction was 7 days shorter for DAC-7 compared to the DA-7 group (33 vs 40 days, P=0.002). Toxicity was comparable in both groups. The probability of 3-year leukemia-free survival (LFS) for DAC-7 and DA-7 group equaled 43 and 34%, respectively (P=NS). There was a trend toward higher LFS rate for patients aged >40 years receiving DAC-7 compared with DA-7 regimen (44 vs 28%, P=0.05). This study proves that addition of 2-CdA increases antileukemic potency of DNR+AraC regimen, thus resulting in a higher CR rate after one induction cycle when compared to DA-7, without additional toxicity. It shortens hospitalization time and may improve long-term survival in patients aged >40 years.Leukemia 06/2004; 18(5):989-97. · 9.56 Impact Factor -
Article: Quantitative ultrastructural evaluation of satellite cells in soleus muscle from rats kept in hypokinesia.
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ABSTRACT: The aim of the present study is a morphometric, ultrastructural evaluation of satellite cells (SC) derived from the soleus muscle (SOL) of rats exposed to conditions of hypokinesia for a period of 7 or 21 days. Qualitative and quantitative analysis of 320 electron micrographs of SC from each group was carried out. After 7 and 21 days of immobilization, profiles of the SC, in contrast to the control group, had a lower mean surface area, had a cylindrical shape, and exhibited more folded membrane. Analysis of electron micrographs of SC showed that after immobilization, a lower number of SC contained profiles of mitochondria, rough endoplasmic reticulum (RER), and Golgi. Volume fractions of RER were twofold lower after 7 days of hypokinesia and fivefold lower after 21 days compared with the control group. The SC of SOL of rats subjected to hypokinesia differed from the control group by a markedly decreased number of ribosomes and RER profiles. After 21 days of immobilization the ultrastructural characteristics of SC were typical for cells in an inactive state showing various degrees of degeneration. The results of our study presented here permit the conclusion that 21 days of hypokinesia induce a depression of SC activity, whereas subtle changes in SC appear only after 7 days of immobilization.Experimental and Molecular Pathology 03/1997; 64(1):13-21. · 2.42 Impact Factor -
Article: Morphometric analysis of satellite cells in rat skeletal muscles: soleus and extensor digitorum longus.
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ABSTRACT: Satellite cells (SC) are present in all types of skeletal muscles. They can exhibit DNA replication and mitotic divisions throughout their life. Depending on the kind of muscle, a different number of SC is present. The aim of this study was to establish whether the differences in muscle metabolism are reflected in the quantitative ultrastructural evaluation of their SC. Two skeletal muscles of rats, soleus (SOL) with predominant slow fibers and extensor digitorum longus (EDL) with predominant fast ones, were studied. Morphometric analysis was performed according to the modified method of Weibel [Stereological Methods, vol 1: Practical Methods for Biological Morphometry. London, Academic Press, 1979]. In the SC of EDL mitochondria, rough endoplasmic reticulum and Golgi apparatus were less frequent as compared with the SOL. The volume fractions of these organelles were much higher in SC of SOL than in EDL. The ribosomes and polyribosomes were more densely packed in SC of SOL than in EDL. Ultrastructural morphometric evaluation demonstrated significant differences between the SC components of two different muscles: a slow (SOL) and a fast (EDL) one.Acta Anatomica 02/1997; 160(2):88-94. -
Article: Morphometric ultrastructural evaluation of satellite cells in musculus soleus and musculus extensor digitorum longus of rats subjected to hypokinesia.
Journal of gravitational physiology: a journal of the International Society for Gravitational Physiology 10/1996; 3(2):16-7. -
Article: Stereological, ultrastructural analysis of Sertoli cells of male mouse embryos.
Folia morphologica 02/1996; 55(4):287-9. · 0.52 Impact Factor -
Article: Morphometrical, ultrastructural study of satellite cells derived from musculus extensor digitorum longus in rats subjected to hypokinesia (7, 14 and 21 days).
Folia morphologica 02/1996; 55(4):472-4. · 0.52 Impact Factor -
Article: Ultrastructural, stereological evaluation of satellite cells derived from musculus soleus in rats kept in hypokinesia conditions.
Folia morphologica 02/1996; 55(4):208-10. · 0.52 Impact Factor -
Article: Morphometric ultrastructural evaluation of satellite cells in musculus gastrocnemius and musculus latissimus dorsi anterior of rats subjected to 7 day hypokinesia.
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ABSTRACT: The investigations were undertaken to evaluate morphometrically the satellite cells (SC) of two muscles: the slow, musclus latissimus dorsi and the fast, musculus gastrocnemius from rats kept in hypokinetic conditions for seven days. The changes in both muscle were of similar character, they concerned a decrease in the Golgi apparatus, rough endoplasmic reticulum fractions and the number of ribosomes, and an increase in the relative volume of heterochromatin content. These findings seem to indicate a reduced activity of the SC. Differences were also demonstrated between the satellite cells in both examined muscles in the control group. They also suggest that the SC are not a homogeneous population as regards parameters evaluated in the present study.Folia morphologica 02/1996; 55(1):21-9. · 0.52 Impact Factor -
Article: Comparative ultrastructural morphometric analysis of satellite cells in three rat muscles: latissimus dorsi, gastrocnemius and quadriceps.
Folia Histochemica et Cytobiologica 02/1996; 34 Suppl 1:29-30. · 0.81 Impact Factor -
Article: Morphometric ultrastructural analysis of germ cells of fetal male mouse gonads.
Folia Histochemica et Cytobiologica 02/1996; 34 Suppl 1:31-2. · 0.81 Impact Factor -
Article: Morphometric ultrastructural study of satellite cells of rats during postnatal development (1st and 30th day of life).
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ABSTRACT: Ultrastructural morphometric analysis of satellite cells of the diaphragm from one and 30-day-old rats was performed. The nuclear and cytoplasmic components of the cells were evaluated. The results obtained indicate that the satellite cells of one-day-old female rats are in a state of low activity. In 30-day-old animals, on the contrary, the cells are excited and active. This may be connected with their intensive participation in the process of development of the diaphragm muscle.Folia morphologica 02/1994; 53(3):137-47. · 0.52 Impact Factor -
Article: Interleukin 2- and interferon alpha induced natural killer cell activity as a marker of progression in hairy cell leukemia.
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ABSTRACT: Hairy cell leukemia (HCL), a rare B-cell chronic lymphoproliferative disorder, is often accompanied by immune abnormalities. A marked impairment of the natural killer cell-mediated cytotoxicity (NK activity) has been reported in most patients at diagnosis. In the present report a long-term follow-up study of NK activity of splenectomized HCL patients is recorded. Among patients who persisted with stable disease two groups, one with normal NK activity, and another with low NK activity, could be recognized. Patients with progressive stage were characterized by a low NK cytotoxic activity. In vitro tests showed that interferon alpha (IFN-alpha) and interleukin 2 (IL2) could increase the NK activity to normal levels only in HCL patients with stable disease, while in progressive HCL these cytokines showed a significantly decreased effect. These results indicate that cytokine-induced NK cytotoxicity appears to be a valuable parameter in assessing the stage of HCL.Leukemia and Lymphoma 04/1993; 9(4-5):371-6. · 2.58 Impact Factor -
Article: Trisomy 13 in a case of myelodysplastic syndrome.
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ABSTRACT: The clinical and cytogenetic findings of a patient with refractory anemia with excess of blasts are presented. Trisomy 13 was present as the sole numerical aberration in all analyzed bone marrow metaphases; this finding has not been reported previously in myelodysblastic syndrome.Cancer Genetics and Cytogenetics 03/1989; 37(2):153-6. · 1.39 Impact Factor -
Article: Morphometric ultrastructural study of the regeneration of soleus muscle in hypokinesia.
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ABSTRACT: The aim of the present study was a morphometric ultrastructural evaluation of regenerating soleus muscle fibers after denervation under conditions of hypokinesia. It was found that in rats kept under conditions of hypokinesia degenerative changes in the soleus muscle after denervation are more pronounced than in the control. On the 36th day after denervation of muscle, symptoms of regeneration of muscle fibers were observed. It was more pronounced in the control than in the experimental groups. Denervation and immobilization applied simultaneously causes more severe degeneration and atrophy of muscle fibers than does the action of only one of these factors.Cell and Tissue Research 02/1983; 232(2):433-43. · 3.11 Impact Factor -
Article: Ultrastructural morphometric evaluation of muscles in pigeons kept under conditions of long-lasting hypodynamic state.
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ABSTRACT: The purpose of this research was to evaluate morphometrically the structure of muscles of pigeons in hypodynamic state, i.e., when they are deprived of flying. The pigeons were allowed to move freely in the cages, thus avoiding a stress reaction. The results of these investigations revealed, that in the 6 months following initiation of the experiment, marked changes appeared in the enzymatic reactions in the mitochondria of the muscle fibers. These changes were reflected in the accumulation of glycogen and fatty droplets. After 12 to 18 months there appeared a limitation of mobility of the muscles followed by an extensive atrophy of the myofibrils with a decreased volume of sarcomeres. The above changes may be interpreted as a consequence of lack of tissue oxygen, aberration in the catabolic pathways of carbohydrates, and diminished content of ATP necessary for proper metabolic functions.Experimental and Molecular Pathology 09/1982; 37(1):26-36. · 2.42 Impact Factor -
Article: Morphometrical ultrastructural evaluation of the cytoplasmic components in acute lymphoblastic leukaemias of T-cell type.
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ABSTRACT: Immunological and ultrastructural morphometric evaluation of the cytoplasmic components of cells was performed in five cases of acute lymphoblastic leukaemia of the T-cell type. All cases were a uniform group as regards clinical and immunological evaluation. On the basis of the mean cell diameter, the volume fractions and true volumes of cell organelles, cells from various cases of acute lymphoblastic leukaemia were found to be no homogeneous group. The cases examined were most homogeneous as far as the volume fraction of the Golgi apparatus and the true volumes of the cell nucleus, mitochondria, lysosomes, smooth and rough endoplasmic reticulum and Golgi apparatus were concerned.Folia haematologica (Leipzig, Germany: 1928) 01/1982; 109(5):716-25. -
Article: [Morphometric ultrastructural evaluation of T-cell lymphoblastic leukemia].
Acta haematologica Polonica 14(1):1-11. -
Article: [Relation between the duration of the chronic phase of chronic myeloid leukemia and total dose of busulfan in the first year of the treatment].
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ABSTRACT: The analysis was based on 31 patients with chronic granulocytic leukaemia (CGL) treated during the period 1975-1986 and the correlation between the total dose of busulphan administered during the first year of treatment and the duration of chronic phase of the disease was investigated. We found such correlation and also we found that the duration of the chronic phase was more significantly correlated with one year maintenance dose of busulphan. This suggests that the prognosis in CGL is more connected with the proliferation activity of leukaemic cells than with the extent of disease at the time of diagnosis.Acta haematologica Polonica 21(1):60-5.
