Li-Ping Jiang

Nanjing University, Nan-ching, Jiangsu Sheng, China

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Publications (49)235.12 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: A simple cyclic voltammetric method has been applied to assemble and orient a model protein, Myoglobin (Mb), into a biocompatible Brij 56 film. Ultra-visible (UV-vis) and circular dichroism (CD) spectra indicated that Mb in Brij 56 matrix preserved its secondary structure. Fourier transform infrared spectra (FT-IR) confirmed the formation of hydrogen bonds between Mb and Brij 56. These hydrogen bonds were acted as the electron tunnel to transfer electrons from Mb's active sites to the underlying glassy carbon electrode. Effective direct electron transfer (DET) of Mb was realized with the presence of a couple of quasi-reversible and well-defined redox peaks at -310 mV (vs. SCE) in the studied potential range. The peaks were attributed to the redox couple of heme Fe(II)/ Fe(III) of the well-oriented Mb in Brij 56 matrix. The surface coverage and the electron transfer rate (ks) of Mb immobilized into the Brij 56 film was about 4.9×10-11 mol cm-2 and 72.6 3.0 s-1, respectively. An excellent electrocatalytic response of the immobilized Mb towards nitrite in the absence of electron transfer mediators was observed. These results emphasized that the biomimetic Brij 56 could be used as an attractive material for immobilizing proteins and constructing biosensors.
    ACS Applied Materials & Interfaces 05/2015; DOI:10.1021/acsami.5b01492 · 5.90 Impact Factor
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    ABSTRACT: A novel "signal-on" photoelectrochemical (PEC) biosensor for sensitive detection of HTLV-II DNA was developed based on enzymatic amplification coupled with terminal deoxynucleotidyl transferase (TdT)-mediated extension strategy. The intensity of the photocurrent signal was proportional to the concentration of the HTLV-II DNA-target DNA (tDNA) by dual signal amplification. In this protocol, GR-CdS:Mn/ZnS nanocomposites were used as photoelectric conversion material, while pDNA was used as the tDNA recognizing unit. Moreover, the TdT-mediated extension and the enzymatic signal amplification technique were used to enhance the sensitivity of detection. Using this novel "signal-on" dual signal amplification strategy, the prototype of PEC DNA sensor can detect as low as sub-femtomolar HTLV-II DNA with excellent differentiation ability even for single-base mismatches. This PEC DNA assay opens a promising platform to detect various DNA targets at ultra-low levels for early diagnoses of different diseases.
    Analytical Chemistry 04/2015; 87(9). DOI:10.1021/acs.analchem.5b00679 · 5.83 Impact Factor
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    ABSTRACT: Global characterization and in-depth understanding of phosphoproteome based on mass spectrometry (MS) desperately needs a highly efficient affinity probe during sample preparation. In this work, a ternary nanocomposite of magnetite/ceria co-decorated titanoniobate nanosheet (MC-TiNbNS) was synthesized by electrostatic assembly of Fe3O4 nanospheres and in situ growth of CeO2 nanoparticles on pre-exfoliated titanoniobate, and eventually utilized as the probe and catalyst for enrichment and dephosphorylation of phosphopeptides. The two-dimensional (2D) structured titanoniobate nanosheet not only promoted the efficacy of capturing phosphopeptides with enlarged surface area, but also functioned as a substrate for embracing magnetic anchor Fe3O4 to enable magnetic separation and mimic phosphatase CeO2 to produce identifying signatures of phosphopeptides. Compared to single component TiNbNS or CeO2 nanoparticles, the ternary nanocomposite provided direct evidence on the number of phosphorylation sites while maintaining the enrichment efficiency. Moreover, by altering on-sheet CeO2 coverage, the dephosphorylation activity could be fine tuned, generating continuously adjustable signal intensities of both phosphopeptides and their dephosphorylated tags. Exhaustive detection of both mono- and multi-phosphorylated peptides with precise counting of their phosphorylation sites were achieved in the primary mass spectra in the cases of digests of standard phosphoprotein and skim milk, as well as more complex biological sample, human serum. With the resulting highly informative mass spectra, this multifunctional probe can be used as a promising tool for fast and comprehensive characterization of phosphopeptides in MS-based phosphoproteomics.
    ACS Applied Materials & Interfaces 03/2015; 7(18). DOI:10.1021/acsami.5b01006 · 5.90 Impact Factor
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    ABSTRACT: A facile and rapid strategy was developed for the synthesis of ultrabright luminescent carbon nanodots (CDs) with tunable wavelength from 464 to 556 nm by introducing glutaraldehyde into the solution under microwave irradiation. The fluorescence properties, including excitation and emission wavelength, quantum yield (QY), and size of the CDs, were adjusted by changing the amount of glutaraldehyde and poly (ethylenimine). Several methods such as UV-Vis, fluorescence, and Fourier transform infrared (FTIR) spectroscopy, high resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), and dynamic light scattering (DLS) were used to characterize the morphology and the properties of the CDs. And the luminescence mechanism was also discussed. In addition, confocal microscopy imaging revealed that the as-prepared CDs could be used as effective fluorescent probes in the imaging of HeLa cells without obvious cytotoxicity. Furthermore, a novel sensor for the detection of Co2+ was proposed on the basis of Co2+-induced fluorescence quenching. These superior properties demonstrated the potential applications of the CDs in cellular imaging and ion sensing.
    ACS Applied Materials & Interfaces 02/2015; DOI:10.1021/am508994w · 5.90 Impact Factor
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    ABSTRACT: MicroRNAs(miRNAs) have emerged as new candidate diagnostic and prognostic biomarkers for the detection of a wide variety of cancers, thus sensitive and selective detection of microRNAs is significant for early-phase cancers diagnosis and disease prevention. A novel and simple electrochemical miRNAs biosensor was developed using Cd2+ modified titanium phosphate nanoparticles as signal unit, two DNA as capture probes and Ru(NH3)63+ as electron transfer mediator. Large quantities of cadmium ions were mounted in titanium phosphate spheres to output the electrochemical signal. Because of the presence of Ru(NH3)63+ molecules which interacted with DNA base-pairs as electron wire, the electrochemical signal significantly increased more than 5 times. This approach achieved a wide dynamic linear range from 1.0 aM to 10.0 pM with an ultra-low limit detection of 0.76 aM, exerting a substantial enhancement in sensitivity. Moreover, the proposed biosensor was sufficiently selective to discriminate the target miRNAs from homologous miRNAs and could be used for rapid and direct analysis of miRNAs in human serum. Therefore, this strategy provides a new and ultrasensitive platform for miRNA expression profiling in biomedical research and clinical diagnosis.
    ACS Applied Materials & Interfaces 01/2015; 7(4). DOI:10.1021/am508690x · 5.90 Impact Factor
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    ABSTRACT: Upconversion nanophosphors (UCNPs) are extremely useful for analytical applications, since they display a high signal-to-noise ratio, and the photobleaching can be ignored. Herein, a novel upconversion nanocomposite composed of β-cyclodextrin (β-CD) derivative modified UCNPs and rhodamine B (RB),was prepared for the detection of cholesterol (Cho). The upconversion luminescence (UCL) emission can serve as a Cho-sensing signal by an effective fluorescence resonance energy transfer (FRET) process, using UCNPs as the donor and RB as the quencher. The sensor for Cho detection in human serum shows excellent sensitivity and selectivity, which has the potential for clinical applications in the analysis of other biological and environmental samples.
    Nanoscale 10/2014; DOI:10.1039/C4NR04380D · 6.74 Impact Factor
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    ABSTRACT: An aminoethoxy-functionalized zirconium phosphonate (Zr(O3POCH2CH2NH2)(2)center dot 3H(2)O), abbreviated as ZrRP (R=OCH2CH2NH2); with layered structure has been synthesized. This layered compound possesses the characteristic of inorganic-organic hybrid, due to the covalently linked aminoethoxy in the host layer. The anion exchanged property of this zirconium phosphonate is suitable for the direct intercalation of negatively charged DNA, which is different from these reported zirconium phosphates or zirconium phosphonates. As a precursor, this prepared zirconium phosphonate was utilized to fabricate a novel DNA/ZrRP binary hybrid via a delamination-reassembly procedure. The release behavior of DNA from the DNA/ZrRP composite was investigated at different medium pH, because the combination between zirconium phosphonate sheets and DNA was pH-dependent sensitively. Moreover, the helical conformation of DNA was almost retained after the intercalation and release process. These properties of the DNA/ZrRP composite suggested the potential application of layered zirconium phosphonate as a non-viral vector in gene delivery.
    Journal of Solid State Chemistry 07/2014; 215:74–79. DOI:10.1016/j.jssc.2014.03.015 · 2.20 Impact Factor
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    ABSTRACT: A surprising electrochemiluminescence (ECL) enhancement effect in graded-gap CdSeTe@ZnS-SiO2 quantum dot (QD) bilayers was observed and used to create an ultrasensitive immunoassay. CdSeTe@ZnS-SiO2 QDs of two different sizes were used as a donor-acceptor pair, owing to their tunable energy and low biotoxicity. The graded-gap CdSeTe@ZnS-SiO2 QD bilayers were fabricated by layer-by-layer assembly of differently sized CdSeTe@ZnS-SiO2 QDs on a glutaraldehyde-activated electrode. Benefiting from a short interlayer distance and perfect spectral overlap in the graded-gap QD bilayers, highly efficient ECL resonance energy transfer (ECLRET)-based energy funneling was observed, wherein excitons from trapped states could be effectively recycled. Consequently, the observed ECL enhancement for the bilayers was more than four times greater than that observed for reference samples. The graded-gap QD bilayers were utilized in an ECL biosensor for the detection of carcinoembryonic antigen (CEA). The proposed method featured a detection limit of 0.4 pg mL(-1) CEA with a linear calibration range from 1 pg mL(-1) to 200 ng mL(-1). This method represents a novel approach for versatile detection of biomolecules in research and clinical applications.
    Analytical Chemistry 03/2014; 86(7). DOI:10.1021/ac500351d · 5.83 Impact Factor
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    ABSTRACT: We report on a sensitive electrochemical immunoassay for the prostate specific antigen (PSA). An immunoelectrode was fabricated by coating a glassy carbon electrode with multiwalled carbon nanotubes, poly(dimethyldiallylammonium chloride), CeO2 and PSA antibody (in this order) using the layer-by-layer method. The immunosensor is then placed in a sample solution containing PSA and o-phenylenediamine (OPD). It is found that the CeO2 nanoparticles facilitate the electrochemical oxidation of OPD, and this produces a signal for electrochemical detection of PSA that depends on the concentration of PSA. There is a linear relationship between the decrease in current and the concentration of PSA in the 0.01 to 1,000 pg mL−1 concentration range, and the detection limit is 4 fg mL−1. The assay was successfully applied to the detection of PSA in serum samples. This new differential pulse voltammetric immunoassay is sensitive and acceptably precise, and the fabrication of the electrode is well reproducible. Figure A novel electrochemical immunoassay for prostate specific antigen (PSA) was developed. Ceria (CeO2) mesoporous nanospheres facilitated the electrochemical oxidation of o-phenylenediamine (OPD). The developed immunoassay has high sensitivity and can be successfully applied for the detection of PSA in serum samples
    Microchimica Acta 03/2014; 181(13-14). DOI:10.1007/s00604-014-1193-x · 3.72 Impact Factor
  • Ying-Di Zhu, Juan Peng, Li-Ping Jiang, Jun-Jie Zhu
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    ABSTRACT: A novel fluorescent immunosensor was developed based on the use of CuS nanoparticles (CuS NPs) as labels for the highly sensitive detection of human prostate cancer biomarker prostate specific antigen (PSA). In the presence of CuS NPs, the non-fluorescent substrate o-phenylenediamine could be oxidized into the stable fluorescent product 2,3-diamiophenazine at physiological pH. Throughout the reaction, no other oxidizing agents (e.g. hydrogen peroxide) were needed. The relatively mild oxidation conditions made the immunoassay robust, reliable and facile. The proposed immunoassay exhibited high sensitivity and specificity for the detection of PSA. A linear relationship between the fluorescent signals and the concentration of PSA was obtained in the range of 0.5 pg mL(-1) to 50 ng mL(-1), with a detection limit of 0.1 pg mL(-1) (S/N = 3). The proposed fluorescent immunoassay can be used as a promising platform for the detection of a variety of other biomarkers.
    The Analyst 12/2013; 139(3). DOI:10.1039/c3an01987j · 3.91 Impact Factor
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    ABSTRACT: A multifunctional boron nitride-gold nanocluster composite was fabricated using poly-diallyldimethylammonium chloride as a stabilizer and a linker. The as-fabricated composite could be used as a fluorescent or an electrochemical label for immunosensing in the sensitive detection of interleukin-6.
    Chemical Communications 10/2013; DOI:10.1039/c3cc45759a · 6.72 Impact Factor
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    ABSTRACT: Graphene quantum dots (GQDs) with green fluorescence were incorporated onto hexagonal boron nitride sheets (HBN) through electrostatic interaction by using poly(diallyldimethylammonium) (PDDA) as the bridge to fabricate the novel nanocomposites (HBN-GQDs). The HBN-GQDs nanocomposites exhibited strong green fluorescent property, high stability, water solubility, very low cytotoxicity on Hela cells. These properties make the HBN-GQDs nanocomposites as good candidate materials for biological applications. The results for the imaging of live cells indicated that the cell-penetrating HBN-GQDs could be a promising nanoprobe for intracellular imaging and therapeutic applications.
    Journal of Biomedical Nanotechnology 10/2013; 9(10):1679-85. DOI:10.1166/jbn.2013.1663 · 7.58 Impact Factor
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    ABSTRACT: In this paper, hemoglobin/DNA/layered double hydroxide composites were fabricated by co-intercalating hemoglobin (Hb) and DNA in the interlayer galleries of Ni–Al layered double hydroxides (Ni–Al LDH) via a delamination-reassembly procedure. The presence of DNA not only remarkably facilitated the intercalation of Hb into the interlayers of the Ni–Al LDH, but also improved the bioactivity of the entrapped Hb. By depositing the Hb/DNA/LDH composite film onto a glassy carbon electrode, a mediator-free biosensor was obtained. Because of the synergistic effect between the LDH host and the co-intercalated DNA guest, the Hb/DNA/LDH composites modified electrode exhibited a fast direct electron transfer with a rate constant, ks, of 6.95 ± 3.31 s−1 and a sensitive electrocatalytic response to H2O2 and NO2− in the linear range of 4.85 × 10−7 to 1.94 × 10−4 M and 2.5 × 10−7 to 3.0 × 10−5 M, respectively. Furthermore, with the protective effect provided by the LDH nanosheets, the electrocatalytic response of the intercalated Hb to H2O2 was retained, even at high temperatures (85 °C) or in the presence of an organic solvent (CH3CN).
    Analytical methods 06/2013; 5(14):-. DOI:10.1039/C3AY00038A · 1.94 Impact Factor
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    ABSTRACT: Haemin-functionalised magnetic iron(II, III) oxide (Fe3O4) nanoparticles (Fe3O4/haemin) were synthesised by changing the acidity of a solution of the two compounds. The nanoparticles were characterised by transmission electron microscopy, UV-vis absorption spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, measurement of magnetisation, and electrochemical techniques. The properties of both haemin and Fe3O4 were retained. Thus, Fe3O4/haemin nanoparticles exhibited pronounced electrocatalytic activity towards trichloroacetic acid (TCA) like haemin itself. Interestingly, electrocatalytic activity towards TCA was affected by detection temperature, which was controlled via electrically heated carbon paste electrodes. The maximal catalytic current was 5.8 times higher at 60°C than at room temperature (25°C). This proposed electrochemical sensor for TCA possessed a linear detection range of 5-80μM and a detection limit of 0.3μM at 60°C.
    Analytica chimica acta 06/2013; 781C:48-53. DOI:10.1016/j.aca.2013.04.011 · 4.52 Impact Factor
  • Feng Lu, Shanhu Liu, Li-Ping Jiang, Jun-Jie Zhu
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    ABSTRACT: CdSexTe1-x semiconductor alloy nanotubes (with an external diameter of 140 nm and an internal diameter of 100 nm) were successfully prepared based on the sacrificial template of Cd(OH)Cl nanorods, and were further in situ assembled with gold nanoparticles (AuNPs) via a sonochemical approach to form the CdSexTe1−x/Au nanotubes. The prepared CdSexTe1−x/Au nanotubes were characterized by X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy, energy-dispersive spectrometry, and X-ray photoelectron spectra. CdSexTe1−x/Au nanotubes could integrate the advantages of the electronic properties of CdSeTe and the biocompatible properties of AuNPs. A novel biosensor was fabricated after the immobilization of hemoglobin (Hb) on CdSexTe1−x/Au nanotubes. The immobilized Hb exhibited fast direct electron transfer and good electrocatalytic performance to H2O2.
    Journal of Nanoparticle Research 01/2013; 15(1). DOI:10.1007/s11051-012-1401-8 · 2.28 Impact Factor
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    ABSTRACT: A novel multianalyte electrochemical immunoassay was developed for ultrasensitive detection of human cardiopathy biomarkers cardiac troponin I (cTnI) and human heart-type fatty-acid-binding protein (FABP) using metal ion functionalized titanium phosphate nanospheres (TiP-metal ion) as labels. The metal ions could be detected directly through square wave voltammetry (SWV) without metal preconcentration, and the distinct voltammetric peaks had a close relationship with each sandwich-type immunoreaction. The position and size of the peaks reflected the identity and level of the corresponding antigen. The large amount of metal ions loading on the TiP nanospheres greatly amplified the detection signals, and the good biocompatibility of graphene nanoribbons (GONRs) retained good stability for the sandwich-type immunoassay. The proposed immunoassay exhibited high sensitivity and selectivity for the detection of cTnI and FABP. The linear relationships between electrochemical signals and the concentrations of cTnI and FABP were obtained in the range of 0.05 pg/mL-50 ng/mL and 0.05 pg/mL-50 ng/mL, respectively. The detection limits of cTnI and HIgG were 1 and 3 fg/mL (S/N = 3), respectively. Moreover, the immunoassay accurately detected the concentrations of cTnI and FABP in human serum samples, which were demonstrated to have excellent correlations with the standard enzyme linked immunosorbent assay (ELISA) method. The results suggested that the electrochemical immunoassay would be promising in the point-of-care diagnostics application of clinical screening of acute myocardial infarction (AMI) biomarkers.
    Analytical Chemistry 08/2012; 84(18):7810-5. DOI:10.1021/ac301438v · 5.83 Impact Factor
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    ABSTRACT: A novel glutathione (GSH) photoelectrochemical biosensor was fabricated using the newly synthesized graphene-CdS (GR-CdS) nanocomposites. The GR-CdS nanocomposites were prepared by a fast, one-step, aqueous reaction. The as-prepared GR-CdS structure inherited the excellent electron transport of GR and facilitated the spatial separation of photo-generated charge carrier, therefore resulting in the enhanced photocurrent, and making it a promising candidate for developing photoelectrochemical biosensors. The proposed GSH sensor displays satisfactory analytical performance with an acceptable linear range from 0.01 to 1.5 mmol L(-1) with a detection limit of 0.003 mmol L(-1) at a signal-to-noise ratio of 3, and also shows an excellent specificity against anticancer drugs and can be successfully applied for GSH detection in real samples. The as-synthesized GR-CdS nanocomposites exhibited obviously enhanced photovoltaic properties, which could be extended to the detection of other enzymes and biomolecules, thus providing a promising platform for the development of photoelectrochemical biosensors.
    The Analyst 07/2012; 137(16):3697-703. DOI:10.1039/c2an35658a · 3.91 Impact Factor
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    ABSTRACT: Porous titanium phosphate (TiP) nanoparticles with high water solubility have been synthesized by using the surfactant sodium dodecyl sulfate (SDS) as the structure-directing agent. The TiP nanoparticles can be functionalized with Cd(2+) and can be further used as labels for electrochemical detection of proteins due to their excellent ion-exchange property.
    Chemical Communications 05/2012; 48(37):4474-6. DOI:10.1039/c2cc31552a · 6.72 Impact Factor
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    ABSTRACT: Fluorescent gold nanoclusters (AuNCs) were incorporated into porous calcium carbonate spheres through electrostatic interaction. The resulting CaCO(3)/AuNCs hybrid material exhibited interesting properties, such as porous structure, excellent biocompatibility, good water solubility, and degradability. These properties make the CaCO(3)/AuNCs hybrid material a promising template to assemble horseradish peroxidase/antibody conjugates (HRP-Ab(2)). By using CaCO(3)/AuNCs/HRP-Ab(2) bioconjugates as probes, a versatile immunosensor was developed for fluorescent and electrochemical detection of the cancer biomarker neuron-specific enolase (NSE). The detection limits of the sensor were 2.0 and 0.1 pg mL(-1) for fluorescent and electrochemical detection, respectively. The immunosensor shows high sensitivity and offers an alternative strategy for the detection of other proteins and DNA.
    Chemistry - A European Journal 04/2012; 18(17):5261-8. DOI:10.1002/chem.201102876 · 5.70 Impact Factor
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    ABSTRACT: Graphene-CdS (GR-CdS) nanocomposites were prepared in a one-step synthesis in aqueous solution. The synthetic approach was simple and fast, and it may be extended for the synthesis of other GR-metal-sulfide nanocomposites. The as-prepared GR-CdS nanocomposite films inherited the excellent electron-transport properties of GR. In addition, the heteronanostructure of the GR-CdS nanocomposites facilitated the spatial separation of the charge carriers, thus resulting in enhanced photocurrent intensity, which makes it a promising candidate for photoelectrochemical applications. This strategy was used for the fabrication of an advanced photoelectrochemical cytosensor, based on these GR-CdS nanocomposites, by using a layer-by-layer assembly process. This photoelectrochemical cytosensor showed a good photoelectronic effect and cell-capture ability, and had a wide linear range and low detection limit for Hela cells. The as-synthesized GR-CdS nanocomposites exhibited obviously enhanced photovoltaic properties, which could be an efficient platform for many other high-performance photovoltaic devices.
    Chemistry - A European Journal 04/2012; 18(16):4974-81. DOI:10.1002/chem.201102379 · 5.70 Impact Factor

Publication Stats

741 Citations
235.12 Total Impact Points

Institutions

  • 2004–2014
    • Nanjing University
      • • Department of Chemical Engineering
      • • Department of Chemistry
      Nan-ching, Jiangsu Sheng, China
  • 2012
    • Henan University
      • Institute of Environmental and Analytical Sciences
      K’ai-feng-shih, Henan Sheng, China