[Show abstract][Hide abstract] ABSTRACT: Onychomycosis is common in immunocompromised patients, but emerging species have been verified, thereby modifying the epidemiological profile of this mycosis. Thus, the aim of this study was to evaluate clinical and mycological profile of onychomycosis among HIV/AIDS patients.
Clinical samples were collected and processed for direct examination, and cultures were maintained at a temperature of 30 °C and 37 °C for 15 days.
Out of 100 patients, 32 had onychomycosis. The etiological agents isolated were Candida albicans, C. parapsilosis, C. tropicalis, C. guilliermondii, Trichophyton rubrum, T. mentagrophytes, Fusarium solani, Scytalidium hialinum, S. japonicum, Aspergillus niger, Cylindrocarpon destructans and Phialophora reptans.
Onychomycosis in HIV/AIDS patients presents various clinical manifestations and may be caused by emerging fungi. The peculiarities presented by different fungal agents justify the need for identification to species level, with the purpose of guiding better therapeutic approaches and minimizing these patients' exposure to conditions presenting a risk of disseminated infection.
Revista da Sociedade Brasileira de Medicina Tropical 02/2011; 44(1):40-2. DOI:10.1590/S0037-86822011000100010 · 0.98 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Hydrolytic enzymes secreted by fungi play an important role in the pathogenesis of infection. With the aim of evaluating the enzymatic activity, 31 isolates of Acremonium stored in the University of Recife Mycology (URM) Culture Collection were tested. Culture fragments were transferred to glycoside broth for reactivation and further growth in potato dextrose agar medium in order to investigate viability and purity and to confirm the taxonomy through observing the macroscopic and microscopic characteristics. To detect enzymes, milk casein and gelatin were used as substrates for proteinase, starch for amylase and soy lecithin for phospholipase. Among the 31 cultures, 26 (83.9%) remained viable and 24 (92.3%) were confirmed taxonomically. Out of these 24 cultures, 12 (50%) presented proteinase activity, of which two (16.7%) were on milk casein, one (8.3%) on gelatin and nine (75%) on both substrates; 16 (66.7%) degraded starch. None of the cultures presented phospholipase activity. It was concluded that Acremonium species are able to produce enzymes that are involved in the pathogenicity of fungal infections.
Revista da Sociedade Brasileira de Medicina Tropical 02/2009; 42(1):63-6. · 0.98 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: This study had the aim of isolating and identifying keratinophilic fungi from soil and correlating them with dermatomycosis agents. According to our results, the predominance of Trichophyton tonsurans as a dermatomycosis agent among children in the city of Recife, PE, is probably due to children's greater contact with soil.
Revista da Sociedade Brasileira de Medicina Tropical 01/2009; 42(4):471-3. DOI:10.1590/S0037-86822009000400023 · 0.98 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Out of a total of 1,238 cases of dermatophytosis in the city of Recife (Pernambuco), lesions of the scalp (33.7%) and Trichophyton tonsurans (25.5%) predominated between 1995 and 2000, while lesions of the hairless skin (35.5%) and Trichophyton rubrum (34%) were the most frequent between 2000 and 2005. A significant reduction in Trichophyton mentagrophytes was detected in the second of these periods.
Revista da Sociedade Brasileira de Medicina Tropical 07/2007; 40(4):484-6. · 0.98 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: This paper reports an unusual case of paracoccidioidomycosis with ulcerations in the head and nose in a Brazilian man. For diagnosis, direct microscopic examination of ulcer secretion and skin samples treated with potassium hydroxide was performed. The isolation of Paracoccidioides brasiliensis occurred at 28 degrees C and 37 degrees C on Sabouraud agar with chloranphenicol. Diagnosis was established by the presence of isolated and multiple budding yeast-like cells and typical fungus growth of P. brasiliensis.
[Show abstract][Hide abstract] ABSTRACT: Opportunistic mycoses have been increasingly observed among immunocompromised patients. We describe a case in which Engyodontium album was isolated and cultured from the blood of a patient with the Acquired Immunodeficiency Syndrome. E. album grew at 37 degrees C and showed proteinase activity, both indicators of pathogenicity. This is the first time that this organism has been reported as agent of fungaemia.
[Show abstract][Hide abstract] ABSTRACT: This study investigated the ocurrence of Malassezia species in clinically healthy students and with macules with a slight fawn discoloration and characterized the isolates as to the pathogenicity factors such as growth at 37ºC, lipase, phospholipase and protease detection. Clinical samples were collected from different body sites of one hundred students of different ages and both sexes. The samples, obtained by scrapping the skin surface and the scalp, were treated with potassa and cultured. Cultures were obtained in Petri dishes containing Sabouraud agar medium added of olive oil, incubated at room temperature and at 37ºC. Culture identifications were based in their morphological and physiological properties. Lipase, phospholipase and protease detection was performed in specific media on Petri dishes for formation of a zone. Globose, spherical yeast cells and hypha were investigated by direct microscopy of clinical materials. Malassezia furfur was detected in seven samples and M. sympodialis in four. All Malassezia cultures presented lipase activity, but none was phospholipase positive. Protease activity was observed in two M. furfur and two M. simpodialis isolates.
[Show abstract][Hide abstract] ABSTRACT: The aim of this research was to verify the ability of 15 isolates of Epidermophyton floccosum to perforate hair in vitro and characterize them for pathogenicity factors such as growth at 37ºC and proteinase and phospholipase production. Fourteen isolates perforated hair and from these twelve produced perforating organs. All isolates grew at 37ºC and produced proteinase, but not phospholipase. These results suggest that E. floccosum may be a possible aethiological agent of tinea capitis.