Wen-Ming Chen

National Kaohsiung Marine University, Kao-hsiung-shih, Kaohsiung, Taiwan

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Publications (170)396.28 Total impact

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    ABSTRACT: A bacterial strain designated TNR-22T was isolated from freshwater river in Taiwan and characterized using the polyphasic taxonomy approach. Cells of strain TNR-22T were faculative anaerobic, Gram-stain-negative, rod-shaped, motile by a single polar flagellum and forming cream colored colonies. Growth occurred at 4-45 °C (optimum, 25-30 °C), with 0-1.0 % NaCl (optimum, 0.5 %) and at pH 7.0-8.0 (optimum, pH 7.0). Strain TNR-22T did not form nodules on a legume plant, Macroptilium atropurpureum. The nifH gene encoding denitrogenase reductase was not detected by PCR. The major fatty acids (>10 %) of strain TNR-22T were C18:1 ω7c and C16:0. The DNA G+C content was 60.3 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, an uncharacterized aminoglycolipid and an uncharacterized phospholipid. Comparative analysis of the 16S rRNA gene sequences showed that strain TNR-22T constituted a distinct branch within the genus Rhizobium, showing the highest level of sequence similarity with respect to Rhizobium rosettiformans W3T (96.3 %). Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Rhizobium. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain TNR-22T represents a novel species in the genus Rhizobium, for which the name Rhizobium alvei sp. nov. is proposed. The type strain is TNR-22T (=BCRC 80408T =LMG 26895T =KCTC 23919T).
    International journal of systematic and evolutionary microbiology. 11/2014;
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  • Wen-Ming Chen, Jhen-Ci Chen, Shih-Yi Sheu
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    ABSTRACT: A bacterial strain, designated Jyi-05T, was isolated from a flooded rice field and characterized using a polyphasic taxonomy approach. Cells of strain Jyi-05T were Gram-stain-negative, strictly aerobic, bright yellow-pigmented rods that were motile by gliding. Growth occurred at 10-30 °C (optimum, 30 °C), at pH 6.5-7.5 (optimum, pH 7.0) and with 0-2 % NaCl (optimum, 1 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Jyi-05T belonged to the genus Flavobacterium and was most closely related to Flavobacterium flevense DSM 1076T with sequence similarity of 97.6 %. Strain Jyi-05T contained iso-C15:0 (16.6 %), anteiso-C15:0 (10.8 %), summed feature 3 (C16:1ω7c and/or C16:1ω6c; 10.3 %), iso-C17:0 3-OH (9.2 %), C16:0 (7.3 %) and iso-C15:0 3-OH (7.2 %) as the predominant fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major polyamine was homospermidine. Genomic DNA G+C content of strain Jyi-05T was 34.2 mol%. The DNA-DNA relatedness of strain Jyi-05T with respect to recognized species of the genus Flavobacterium was less than 70 %. On the basis of the phylogenetic inference and phenotypic data, strain Jyi-05T should be classified as a novel species, for which the name Flavobacterium oryzae sp. nov. is presented. The type strain is Jyi-05T (=BCRC 80522T =LMG 27292T =KCTC 32240T). Emended descriptions of Flavobacterium flevense, Flavobacterium yonginense and Flavobacterium myungsuense are also proposed.
    International journal of systematic and evolutionary microbiology. 08/2014;
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    ABSTRACT: The South African invasive legume Dipogon lignosus (Phaseoleae) produces nodules with both determinate and indeterminate characteristics in New Zealand (NZ) soils. Ten bacterial isolates produced functional nodules on D. lignosus. The 16S ribosomal RNA (rRNA) gene sequences identified one isolate as Bradyrhizobium sp., one isolate as Rhizobium sp. and eight isolates as Burkholderia sp. The Bradyrhizobium sp. and Rhizobium sp. 16S rRNA sequences were identical to those of strains previously isolated from crop plants and may have originated from inocula used on crops. Both 16S rRNA and DNA recombinase A (recA) gene sequences placed the eight Burkholderia isolates separate from previously described Burkholderia rhizobial species. However, the isolates showed a very close relationship to Burkholderia rhizobial strains isolated from South African plants with respect to their nitrogenase iron protein (nifH), N-acyltransferase nodulation protein A (nodA) and N-acetylglucosaminyl transferase nodulation protein C (nodC) gene sequences. Gene sequences and enterobacterial repetitive intergenic consensus (ERIC) PCR and repetitive element palindromic PCR (rep-PCR) banding patterns indicated that the eight Burkholderia isolates separated into five clones of one strain and three of another. One strain was tested and shown to produce functional nodules on a range of South African plants previously reported to be nodulated by Burkholderia tuberum STM678(T) which was isolated from the Cape Region. Thus, evidence is strong that the Burkholderia strains isolated here originated in South Africa and were somehow transported with the plants from their native habitat to NZ. It is possible that the strains are of a new species capable of nodulating legumes.
    Microbial Ecology 05/2014; · 3.28 Impact Factor
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    ABSTRACT: A bacterial strain designated KBP-21T was isolated from a water sample taken from the Banping Lake Wetland Park in Taiwan and characterized using the polyphasic taxonomic approach. Cells of strain KBP-21T were Gram-staining-negative, facultatively anaerobic, poly-β-hydroxybutyrate-accumulating, motile rods and forming yellow-colored colonies. Growth occurred at 15-40 oC (optimum, 30 °C), at pH 5.0-8.0 (optimum, pH 8.0) and with 0-2 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KBP-21T belonged to the genus Paludibacterium within the family Neisseriaceae of the class Betaproteobacteria and its most closely related neighbour was Paludibacterium yongneupense 5YN8-15T with sequence similarity of 96.4 %. Strain KBP-21T contained summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), C16:0 and C18:1 ω7c as the predominant fatty acids. The major respiratory quinone was Q-8. The DNA G+C content of the genomic DNA was 62.1 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one uncharacterized aminophospholipid and several uncharacterized phospholipids. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain KBP-21T represents a novel species in the genus Paludibacterium, for which the name Paludibacterium paludis sp. nov. is proposed. The type strain is KBP-21T (=BCRC 80514T =LMG 27230T =KCTC 32182T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2014; · 2.11 Impact Factor
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    ABSTRACT: A bacterium strain, designated Npb-03T was isolated from freshwater river in Taiwan and was characterized using the polyphasic taxonomy approach. The cells were Gram-negative, straight rod-shaped, non-motile, non-spore forming and faculatively anaerobic. Growth occurred at 10-37 °C (optimum, 30-35 °C), at pH 6.0-8.0 (optimum, pH 6.0-7.0) and with 0-1.0 % NaCl (optimum, 0 %). The predominant fatty acids were summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c) and C16:0. The major isoprenoid quinone was Q-8 and the DNA G+C content was 64.1 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminolipid and three uncharacterized phospholipids. The major polyamines were putrescine, 2-hydroxyputrescine, cadaverine and spermidine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Npb-03T forms a distinct lineage with respect to closely related genera within the family Neisseriaceae of the class Betaproteobacteria, most closely related to Aquaspirillum, Laribacter, Leeia and Microvirgula, and the levels of 16S rRNA gene sequence similarity with respect to the type species of related genera are less than 93 %. On the basis of the genotypic and phenotypic data, strain Npb-03T represents a novel genus and species of the family Neisseriaceae, for which the name Rivicola pingtungensis gen. nov., sp. nov. is proposed. The type strain is Npb-03T (=BCRC 80376T =LMG 26668T =KCTC 23712T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2014; · 2.11 Impact Factor
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    ABSTRACT: A continuous fixed-bed biosorption process was established for cadmium (Cd) removal by Scenedesmus obliquus CNW-N (isolated from southern Taiwan) cells immobilized onto loofa sponge. This immobilized-cell biosorption process allows better recovery and reusability of the microalgal biomass. The growth of microalgae on the matrix support with appropriate nutrient supplementation could enhance the overall metal removal activity. Major operating parameters (e.g., feeding flow rate, cycle number of medium replacement, and particle diameter of the sponge) were studied for treatability evaluation. The most promising cell growth on the sponge support was obtained at a flow rate of 0.284 bed volume (BV)/min, sponge particle diameter of 1cm, and with one cycle of medium replacement. The performance of fixed-bed biosorption (adsorption capacity of 38.4mg, breakthrough time at 15.5h) was achieved at a flow rate of 5ml/min with an influent concentration of 7.5mgCd/l.
    Bioresource Technology 02/2014; · 5.04 Impact Factor
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    ABSTRACT: A bacterial strain designated CMJ-9T was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain CMJ-9T were strictly aerobic, Gram-negative, motile by a single polar flagellum, poly-β-hydroxybutyrate-containing and forming light yellow-pigmented colonies. Growth occurred at 10-37 °C (optimum, 20-30 °C), with 0-0.8 % NaCl (optimum, 0-0.1 %) and at pH 6.0-9.0 (optimum, pH 6.0-7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CMJ-9T belonged to the genus Undibacterium and its closest neighbour was Undibacterium seohonense SHS5-24T with sequence similarities of 96.7 %. The predominant cellular fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and C16:0. The major cellular hydroxy fatty acid was C10:0 3-OH. The polar lipid profile consisted of the predominant lipids phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The polyamine profile was composed of the major compound putrescine and moderate amounts of 2-hydroxyputrescine. The major respiratory quinone was Q-8 and the DNA G+C content was 47.7 mol%. On the basis of the phylogenetic and phenotypic data, strain CMJ-9T should be classified as representing a novel species, for which the name Undibacterium macrobrachii sp. nov. is proposed. The type strain is CMJ-9T (=BCRC 80406T =LMG 26891T =KCTC 23916T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2013; · 2.11 Impact Factor
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    ABSTRACT: Variations in the patterns of diversity of symbionts have been described worldwide on Mimosa pudica, a pan-tropical invasive species that interacts with both α and β-rhizobia. In this study, we investigated if symbiont competitiveness can explain these variations and the apparent prevalence of β- over α-rhizobia. We developed an indirect method to measure the proportion of nodulation against a GFP reference strain and tested its reproducibility and efficiency. We estimated the competitiveness of 54 strains belonging to four species of β-rhizobia and four of α-rhizobia, and the influence of the host genotype on their competitiveness. Our results were compared with biogeographical patterns of symbionts and host varieties. We found: (i) a strong strain effect on competitiveness largely explained by the rhizobial species, with Burkholderia phymatum being the most competitive species, followed by B. tuberum, whereas all other species shared similar and reduced levels of competitiveness; (ii) plant genotype can increase the competitiveness of Cupriavidus taiwanensis. The latter data support the likelihood of the strong adaptation of C. taiwanensis with the M. pudica var. unijuga and help explain its prevalence as a symbiont of this variety over Burkholderia species in some environments, most notably in Taiwan.
    Environmental Microbiology 09/2013; · 6.24 Impact Factor
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    ABSTRACT: A bacterial strain designated LTC-2T was isolated from a freshwater lake in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain LTC-2T were Gram-negative, facultatively anaerobic, poly-β-hydroxybutyrate accumulating, motile by means of monopolar flagellum, non-spore forming, slightly curved rods surrounded by a thick capsule and forming creamy white-colored colonies. Growth occurred at 10-37 °C (optimum, 20-30 °;C), at pH 6.0-9.0 (optimum, pH 7.0-8.0) and with 0-1.0 % NaCl (optimum, 0 %). The predominant fatty acids were C18:1 ω7c, summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c) and C16:0. The major isoprenoid quinone was Q-10 and the DNA G+C content was 58.5 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylmonomethylethanolamine (PME), phosphatidyldimethylethanolamine (PDE), two uncharacterized phospholipids (PL1 and PL2) and two uncharacterized aminophospholipids (APL1 and APL2). The major polyamines were putrescine, homospermidine and spermidine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain LTC-2T forms a distinct lineage with respect to closely related genera in the family Rhodospirillaceae, most closely related to Elstera and Dongia and the levels of 16S rRNA gene sequence similarity with respect to the type species of related genera are less than 94 %. On the basis of the genotypic and phenotypic data, strain LTC-2T represents a novel genus and species of the family Rhodospirillaceae, for which the name Lacibacterium aquatile gen. nov., sp. nov. is proposed. The type strain is LTC-2T (=BCRC 80445T =LMG 26999T =KCTC 32017T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 09/2013; · 2.11 Impact Factor
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    ABSTRACT: A bacterial strain designated Orc-4T was isolated from a freshwater spring in Taiwan and characterized using the polyphasic taxonomic approach. Cells of strain Orc-4T were facultatively anaerobic, Gram-negative, poly-β-hydroxybutyrate-accumulating, non-motile rods surrounded by a thick capsule and forming cream white-colored colonies. Growth occurred at 15-40 °C (optimum, 25-30 °C), at pH 6.0-9.0 (optimum, pH 7.0) and with 0-1 % NaCl (optimum, 0-0.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Orc-4T belonged to the genus Gemmobacter within the family Rhodobacteraceae of the class Alphaproteobacteria and its most closely related neighbour was Gemmobacter fontiphilus JS43T with sequence similarity of 97.8 %. Strain Orc-4T contained C18:1ω7c (71.0 %) as the predominant fatty acid. The major respiratory quinone was Q-10. The DNA G+C content of the genomic DNA was 63.5 mol%. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one uncharacterized aminolipid and several uncharacterized phospholipids. The DNA-DNA relatedness of strain Orc-4T with respect to recognized species of the genus Gemmobacter was less than 48 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Orc-4T represents a novel species in the genus Gemmobacter, for which the name Gemmobacter lanyuensis sp. nov. is proposed. The type strain is Orc-4T (=BCRC 80378T =LMG 26667T =KCTC 23714T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2013; · 2.11 Impact Factor
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    ABSTRACT: Background and AimsThe large monophyletic genus Mimosa comprises approx. 500 species, most of which are native to the New World, with Central Brazil being the main centre of radiation. All Brazilian Mimosa spp. so far examined are nodulated by rhizobia in the betaproteobacterial genus Burkholderia. Approximately 10 Mya, transoceanic dispersal resulted in the Indian subcontinent hosting up to six endemic Mimosa spp. The nodulation ability and rhizobial symbionts of two of these, M. hamata and M. himalayana, both from north-west India, are here examined, and compared with those of M. pudica, an invasive species.Methods Nodules were collected from several locations, and examined by light and electron microscopy. Rhizobia isolated from them were characterized in terms of their abilities to nodulate the three Mimosa hosts. The molecular phylogenetic relationships of the rhizobia were determined by analysis of 16S rRNA, nifH and nodA gene sequences.Key ResultsBoth native Indian Mimosa spp. nodulated effectively in their respective rhizosphere soils. Based on 16S rRNA, nifH and nodA sequences, their symbionts were identified as belonging to the alphaproteobacterial genus Ensifer, and were closest to the 'Old World' Ensifer saheli, E. kostiensis and E. arboris. In contrast, the invasive M. pudica was predominantly nodulated by Betaproteobacteria in the genera Cupriavidus and Burkholderia. All rhizobial strains tested effectively nodulated their original hosts, but the symbionts of the native species could not nodulate M. pudica.Conclusions The native Mimosa spp. in India are not nodulated by the Burkholderia symbionts of their South American relatives, but by a unique group of alpha-rhizobial microsymbionts that are closely related to the 'local' Old World Ensifer symbionts of other mimosoid legumes in north-west India. They appear not to share symbionts with the invasive M. pudica, symbionts of which are mostly beta-rhizobial.
    Annals of Botany 05/2013; · 3.45 Impact Factor
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    ABSTRACT: A bacterial strain designated TQQ6T was isolated from a freshwater river in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain TQQ6T were strictly aerobic, Gram-staining-negative, poly-β-hydroxybutyrate-containing, non-motile, non-spore forming, long-shaped rods surrounded by a thick capsule and forming pale orange-colored colonies. Growth occurred at 20-40 °C (optimum, 25 °C), at pH 7.0-9.0 (optimum, pH 8.0) and with 0-0.5 % NaCl (optimum, 0 %). The predominant fatty acids were iso-C15:0, summed feature 3 (comprising C16:1ω6c and/or C16:1ω7c), iso-C17:0 3-OH, C16:1ω5c and C16:0. The major isoprenoid quinone was MK-7 and the DNA G+C content was 42.2 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, two uncharacterized aminophospholipids and three uncharacterized phospholipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TQQ6T represents a distinct phyletic line that reflects a novel generic status within the family Cytophagaceae with relatively low sequence similarities (less than 90 %) to members of other genera with validly published names. On the basis of the genotypic and phenotypic data, strain TQQ6T represents a novel genus and species of the family Cytophagaceae, for which the name Fluviimonas pallidilutea gen. nov., sp. nov. is proposed. The type strain is TQQ6T (=BCRC 80447T=LMG 27056T=KCTC 32035T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2013; · 2.11 Impact Factor
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    ABSTRACT: A novel nitrogen-fixing strain, designated DQS-4T was isolated from oil-contaminated soil in Taiwan and was characterized using a polyphasic taxonomy approach. Cells of strain DQS-4T stained Gram-negative, contained poly-β-hydroxybutyrate granules, and were motile rods surrounded by a thin capsule. Cells have a strictly aerobic type of metabolism and fix nitrogen microaerobically. Growth occurred at 10-45 °C (optimum, 35-40 °C), at pH 7.0-8.0 (optimum, pH 7.0) and with 0-2 % NaCl (optimum, 0.5-1 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DQS-4T belonged to the genus Azoarcus, and its closest neighbours were Azoarcus indigens LMG 9092T and Azoarcus communis DSM 12120T, with sequence similarities of 97.4 and 96.4 %, respectively. The major cellular fatty acids of strain DQS-4T were summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c), C16:0 and C18:1ω7c. The major cellular hydroxy fatty acid was C10:0 3-OH. The DNA G+C content was 64.5 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and phospholipids. The mean level of DNA-DNA relatedness between strain DQS-4T and Azoarcus indigens LMG 9092T was 27.4 %. On the basis of the genotypic and phenotypic data, strain DQS-4T represents a new species in the genus Azoarcus, for which the name Azoarcus olearius sp. nov. is proposed. The type strain is DQS-4T (=BCRC 80407T =KCTC 23918T =LMG 26893T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2013; · 2.11 Impact Factor

Publication Stats

2k Citations
396.28 Total Impact Points

Institutions

  • 2004–2014
    • National Kaohsiung Marine University
      Kao-hsiung-shih, Kaohsiung, Taiwan
  • 2012
    • Kaohsiung Municipal Ta-Tung Hospital, Taiwan
      Kao-hsiung-shih, Kaohsiung, Taiwan
  • 2008–2012
    • Justus-Liebig-Universität Gießen
      • Institut für Angewandte Mikrobiologie
      Gießen, Hesse, Germany
    • National Ilan University
      • Department of Chemical and Materials Engineering
      Taiwan
    • Kao Yuan University
      Kao-hsiung-shih, Kaohsiung, Taiwan
    • Tajen University
      • Department of Biotechnology
      P’ing-tung-chieh, Taiwan, Taiwan
  • 2006–2012
    • National Chung Hsing University
      • Department of Soil and Environmental Sciences
      Taichung, Taiwan, Taiwan
  • 2011
    • Yuan Ze University
      • Graduate School of Biotechnology and Bioengineering
      Taoyuan City, Taiwan, Taiwan
    • University of Wisconsin - Milwaukee
      • Department of Biological Sciences
      Milwaukee, WI, United States
    • National Central University
      Taoyuan City, Taiwan, Taiwan
  • 2005–2010
    • National Cheng Kung University
      • Department of Chemical Engineering
      Tainan, Taiwan, Taiwan
    • Feng Chia University
      • Department of Chemical Engineering
      Taichung, Taiwan, Taiwan
  • 2009
    • Yenepoya University
      • Yenepoya Research Centre
      Mangalore, State of Karnataka, India
  • 2007–2008
    • University of Dundee
      • College of Life Sciences
      Dundee, SCT, United Kingdom
    • University of Bonn
      • Institute of Medical Microbiology, Immunology and Parasitology
      Bonn, North Rhine-Westphalia, Germany