Hasan Serdaroglu

Istanbul University, İstanbul, Istanbul, Turkey

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Publications (8)18.34 Total impact

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    ABSTRACT: Objective: To evaluate the association between male age and semen parameters and outcome of assisted reproduction techniques. Design: A retrospective study of the patients attending to the IVF laboratory in a university based infertility clinic. Materials and Methods: 645 couples undergoing IVF/ICSI-ET cycles between 1998 and 2005 were included in this retrospective study. Ovulation inductions, oocyte retrievals, embryo cultures and embryo transfers were done by using standard protocols. Fertilization technique was not taken as a separate classification item. The study population was grouped into three depending on the male age such as <30 years old in Group A, >31 and <44 years old in group B and >45 years old in group C. The evaluated outcomes were semen parameters and fertilization, grade I (good quality) embryo development, number of transferred embryo, arrested embryo and pregnancy rates. All of the semen analyses were done within 3-5 days of abstinence period. Since the semen analyses were done as a part of sperm preparation in the assisted reproductive treatment cycle, motility (percent motile) and sperm count were assessed with light microscope according to WHO criteria and performed just before the fertilization procedure. Data were analyzed using Pearson correlation test, Chi-Square Tests, one-way analyses of variance (ANOVA), and logistic regression model and the Tukey test was used post-hoc for multiple comparisons. P<0.05 was considered statistically significant. All statistical analyses were performed using Windows SPSS version 10.0 software. Results: The mean of male and female ages were 35.1(5.6) and 32.1 (5.1) respectively. Group C was associated with lower semen volume whereas group B with higher total sperm count. The percentage of the MII oocyte fertilization had no correlation either with the male age or female age. The number of the arrested embryos was negatively correlated only with female age (p=0.006). Male and female ages were both negatively correlated with the number of grade I embryos (p<0.0001) and number of transferred embryos (p<0.0001) on day 3. Although female age showed higher odds ratio for pregnancy outcome in a logistic regression model that investigated the interaction of ages on the pregnancy outcome, there were no statistically significance (p<0.071). Conclusions: Male age has a predictive value in semen quality. Although male age is observed as a confounding factor in good embryo development, female age appears as a dominant determinant factor. Financial Support: None
    Fertility and Sterility 09/2007; 88(Supplement 1):S264. · 4.17 Impact Factor
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    ABSTRACT: Angiogenesis, the development of new blood vessels from preexisting capillaries, is essential for the development, growth and advancement of solid tumours. Angiogenesis is enhanced by prostaglandins (PGs) that are synthesised by the catalysis of cyclooxygenases (COX-1 and COX-2) from arachidonic acid. COX-2 is upregulated in a variety of malignancies and favours the growth of malignant cells by stimulating proliferation and angiogenesis. The aim of this study is to investigate the angiogenetic process by determining the levels of vascular endothelial growth factor (VEGF), monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-8 in endometrial cancer cells and to study the effect of nimesulide, a selective COX-2 inhibitor, on these mediators using cell culture. Endometrial tissue specimens were obtained from subjects with endometrial cancer and intramural leiomyoma. Cells were incubated with either 10 or 50 microM nimesulide for 24 h. VEGF, MCP-1 and IL-8 concentrations were determined by sandwich quantitative enzyme immunoassay (ELISA). VEGF concentration was significantly higher in cancer cells than normal endometrial cells. VEGF was decreased with 10 microM nimesulide in cancer cells whereas it remained unaltered in normal cells. Both MCP-1 and IL-8 concentrations were lower in cancer cells than normal cells. MCP-1 levels were decreased with both doses of nimesulide in normal cells, whereas IL-8 levels were significantly affected only by 50 microM of nimesulide. These results suggest that COX-2 inhibitors may be effective in the treatment of endometrial cancer via suppression of angiogenesis.
    Clinical and Experimental Medicine 04/2007; 7(1):6-10. · 2.83 Impact Factor
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    ABSTRACT: This pilot study was conducted to compare the results of intrauterine insemination (IUI) under ovarian stimulation with either letrozole (Femara) or human menopausal gonadotrophin (HMG). A randomized controlled trial was conducted. Eighty women aged 20-35 years with unexplained infertility of at least 2 years' duration were randomized according to a computer-generated randomization list into the letrozole group and the HMG group. Letrozole was administered at 5 mg/day from day 3 to day 7 of the IUI cycle. HMG injections were started on day 3 at a dose of 75 IU for women under 30 years old and 150 IU for women over 30 years old and monitored periodically by vaginal ultrasound and oestradiol concentrations. The variables selected for analysis were clinical pregnancy rate, endometrial thickness, length of follicular phase and number of preovulatory follicles. No statistically significant difference in clinical pregnancy rates per cycle was found for patients in the letrozole or HMG group (18.4 versus 15.7%). Cost was significantly higher in the HMG stimulation cases (P < 0.001) and no injections were required in the letrozole group. In conclusion, letrozole offers a new treatment regimen in ovarian stimulation regimens for IUI that is cost effective, simple and convenient for the patients.
    Reproductive biomedicine online 08/2006; 13(2):208-12. · 2.68 Impact Factor
  • Article: P-394
    Fertility and Sterility - FERT STERIL. 01/2006; 86(3).
  • Fertility and Sterility - FERT STERIL. 01/2005; 84.
  • Fertility and Sterility 01/2004; 80(6):1518-20. · 4.17 Impact Factor
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    ABSTRACT: Leukaemia inhibitory factor (LIF) is expressed at high constitutive levels in the human Fallopian tubal epithelium. In this study, the effect of human recombinant LIF on sperm motility and survival in vitro was investigated. Human spermatozoa were incubated in sperm washing medium that contained various concentrations of LIF at 37 degrees C and under 5% of CO(2) in air for up to 48 h. Sperm motion characteristics were measured using a sperm motility analyser. Sperm survival was determined by the hypo-osmotic swelling test. The effect of LIF on sperm motility was concentration-dependent and maximal effect was observed at a concentration of 5 ng/ml. Sperm motility was significantly higher after 24 h exposure to LIF compared with control (P < 0.001). Sperm survival was also prolonged in a concentration-dependent manner. LIF significantly enhanced sperm survival at higher concentrations (10 ng/ml) and the result was significant after 48 h exposure (P < 0.05). LIF increased long-term sperm motility and survival in vitro.
    Reproductive biomedicine online 01/2003; 7(1):71-4. · 2.68 Impact Factor
  • E Attar, S Gokdemirel, H Serdaroglu, A Coskun
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    ABSTRACT: The Billings ovulation method (BOM) is a medical model of a natural procreation education method based on scientific observation of the changes in the cervical mucus. In this study we examined Turkish women's interest in accurate usage of cervical mucus changes in determining the ovulation time. Fifteen regularly cycling women monitored their fertility patterns in 30 cycles using the BOM and urine luteinizing hormone (LH) kits. At the end ofeach cycle, the cervical mucus monitoring chart and LH kits were collected from the subjects and analyzed. The cervical mucus symptoms defined a potential fertile period of 10 days' average length, with the 'peak' mucus characteristic occurring at a mean of day 13.65 +/- 2.62 of the cycle. The duration ofthe LH surge, as observed in early morning urine samples, averaged 5 days, with the peak occurring at a mean of day 13.40 +/- 2.58 of the cycle. Data indicated that there was a strong correlation between LH in the urine and the peak in self-observed, cervical-vaginal mucus (p = 0.001). This study proved that women can distinguish patterns of ovulation and anovulation by self-detection of variations in cervical mucus characteristics, and that urinary LH levels strongly correlate with ovulation.
    The European Journal of Contraception and Reproductive Health Care 07/2002; 7(2):96-9. · 1.81 Impact Factor

Publication Stats

28 Citations
18.34 Total Impact Points

Institutions

  • 2004–2007
    • Istanbul University
      • • Department of Family Medicine (Istanbul Medical Faculty)
      • • Division of Reproductive Endocrinology and Infertility
      İstanbul, Istanbul, Turkey