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Ireneusz Soltyszewski,
Andrzej Plocienniczak,
Hans Ake Fabricius,
Igor Kornienko,
Dmitrij Vodolazhsky,
Walther Parson,
Roman Hradil,
Hermann Schmitter,
Pavel Ivanov,
Piotr Kuzniar, [......],
Peter de Knijff,
Krzysztof Rebała,
Joanna Wysocka,
Ewa Kapińska, Lidia Cybulska,
Alexei I Mikulich,
Iosif S Tsybovsky,
Zofia Szczerkowska,
Paweł Krajewski,
Rafał Ploski
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ABSTRACT: The purpose of this study was to evaluate the homogeneity of Polish populations with respect to STRs chosen as core markers of the Polish Forensic National DNA Intelligence Database, and to provide reference allele frequencies and to explore the genetic interrelationship between Poland and neighboring countries. The allele frequency distribution of 10 STRs included in the SGMplus kit was analyzed among 2176 unrelated individuals from 6 regional Polish populations and among 4321 individuals from Germany (three samples), Austria, The Netherlands, Sweden, Czech Republic, Slovakia, Belarus, Ukraine and the Russian Federation (six samples). The statistical approach consisted of AMOVA, calculation of pairwise Rst values and analysis by multidimensional scaling. We found homogeneity of present day Poland and consistent differences between Polish and German populations which contrasted with relative similarities between Russian and German populations. These discrepancies between genetic and geographic distances were confirmed by analysis of an independent data set on Y chromosome STRs. Migrations of Goths, Viking influences, German settlements in the region of Volga river and/or forced population resettlements and other events related to World War II are the historic events which might have caused these finding.
Forensic science international. Genetics 07/2008; 2(3):205-11. · 2.42 Impact Factor
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ABSTRACT: Allele frequencies of 15 short tandem repeat loci included in the AmpFlSTR Identifiler kit (Applied Biosystems) were obtained from a sample set of unrelated individuals living in Belarus (n=176). For all loci, no deviation from Hardy-Weinberg equilibrium was found. Results were compared with data available for the Belarusian minority residing in northeastern Poland and for other Slavic populations. Statistically significant differences were observed between Belarusians and all compared populations. The values of heterozygosity, polymorphic information content (PIC), power of discrimination (PD), power of exclusion (PE), paternity index (PI) and matching probability (pM) were calculated.
Forensic science international 01/2008; 173(2-3):235-7. · 2.10 Impact Factor
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ABSTRACT: INTRODUCTION, MATERIALS AND METHODS: The allele frequencies of four short tandem repeats (STR) loci specific to the human X chromosome (DXS101, DXS7423, DXS8377 and phosphoribosyltransferase HPRTB) were analyzed by means of a multiplex PCR reaction in a sample of 200 unrelated individuals residing in the northern part of Poland. The separation and detection of PCR products were performed by capillary electrophoresis on the 3130 Genetic Analyzer. Testing for Hardy-Weinberg equilibrium (HWE) showed no significant deviation for these loci. RESULTS: Statistical parameters such as: heterozygosity observed, mean exclusion chance, power of discrimination in males and power of discrimination in females showed that the examined multiplex is useful in forensic and paternity testing applications.
Annales Academiae Medicae Stetinensis 01/2007; 53 Suppl 2:170-4; discussion 174.
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ABSTRACT: The objective of the study was to examine the mutation rates of Y-chromosomal STR from father-son pairs. The paternity in these cases was confirmed previously with the use of autosomal STR system performing standard analyses of genetic profiles of the mother, child and putative father (PI > = 100000). We examined 200 father-son sample pairs from Northern Poland using the Y-STR 18-plex. We identified eleven mutations. Five mutations resulted in the gain of a repeat in the sons' chromosome and six resulted in a loss of a repeat. All the samples resulted in single repeat mutations from one sample, which contained a two repeat loss at DYS385. The overall average mutation rate estimate was 0.0031.There was no significant difference in the mutation rate between Y-STR loci of the 200 tested father-son pairs and the YHRD base.
Archiwum medycyny sa̧dowej i kryminologii 62(3):147-51.
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ABSTRACT: Besides autosomal STR loci, markers of sex chromosomes, X and Y, are increasingly more commonly used in genetic analyses aiming at paternity testing or personal identification. The paper presents cases in which analysis of microsatellite loci of the X chromosome (X-STRs) was included in the routine examination and allowed for an unambiguous determination of the relationship between the tested individuals. The cases addressed paternity testing of female children, determination whether the examined women were paternal half-sisters, as well as personal identification of a deceased man. In none of the conducted expert opinions, the putative father's DNA sample was't available. Genotyping of X-STR markers was carried out with the use of commercial kits: Mentype Argus X-8 PCR Amplification Kit (Biotype) and Investigator Argus X-12 Kit (Qiagen).
Archiwum medycyny sa̧dowej i kryminologii 62(3):152-9.
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ABSTRACT: This paper presents the results of a population study of a dinucleotide STR marker DXS1062. Blood samples were obtained from unrelated adult individuals (males and females) living in the northern part of Poland. In the analyzed population, 21 different phenotypes and 9 alleles of the DXS1062 locus were found. The alleles were sequenced and used for the construction of an allelic ladder. The nomenclature in accordance with ISFG guidelines was proposed. The most frequent alleles were 20 and 21. Statistical parameters (PR, PM, PD, PIC) showed that the examined system is useful in forensic medicine.
Archiwum medycyny sa̧dowej i kryminologii 54(2-3):95-100.
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ABSTRACT: This paper describes results of a population study of two X-linked STR microsatellite markers: DXS7108 and DXS1196. 298 samples of DNA of unrelated persons (male and female) from the Northern part of Poland were analyzed. DNA was isolated using a non-enzymatic method. After amplification PCR products were separated by means of capillary electrophoresis using the ABI PRISM 310 Genetic Analyzer. The most common alleles of each locus were sequenced and used as a control ladder to type unknown samples. Testing for Hardy-Weinberg equilibrium (HWE) showed no significant deviation for these two loci. Statistical parameters (PD, HET, MEC) showed that examined systems are useful in forensic medicine.
Archiwum medycyny sa̧dowej i kryminologii 55(1):32-5.
