Annamari Ranki

Helsinki University Central Hospital, Helsinki, Uusimaa, Finland

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Publications (219)1157.05 Total impact

  • Journal of the American Academy of Dermatology 06/2015; 72(6):e179. DOI:10.1016/j.jaad.2015.02.1135 · 5.00 Impact Factor
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    ABSTRACT: Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy (APECED) is an autoimmune disorder characterized by chronic mucocutaneous candidiasis, hypoparathyroidism, and adrenal insufficiency, but patients also develop intestinal disorders. APECED is an autosomal recessive disorder caused by mutations in the autoimmune regulator (AIRE, which regulates immune tolerance), that allow self-reactive T cells to enter the periphery. Enteric α-defensins are anti-microbial peptides secreted by Paneth cells. Patients with APECED frequently have gastrointestinal symptoms and sero-reactivity against secretory granules of Paneth cells. We investigated whether enteric α-defensins are autoantigens in humans and mice with AIRE deficiency.
    Gastroenterology 05/2015; 149(1). DOI:10.1053/j.gastro.2015.05.009 · 13.93 Impact Factor
  • 05/2015; DOI:10.1530/endoabs.37.GP.26.08
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    ABSTRACT: Cutaneous lupus erythematosus (CLE) is a chronic autoimmune disease of the skin with typical clinical manifestations. Here, we genotyped 906,600 single nucleotide polymorphisms (SNPs) in 183 CLE cases and 1288 controls of Central European ancestry. Replication was performed for 13 SNPs in 219 case subjects and 262 controls from Finland. Association was particularly pronounced at 4 loci, all with genome-wide significance (P ≤ 5 x 10(-8) ): rs2187668 (PGWAS = 1.4 x 10(-12) ); rs9267531 (PGWAS = 4.7 x 10(-10) ); rs4410767 (PGWAS = 1.0 x 10(-9) ); rs3094084 (PGWAS = 1.1 x 10(-9) ). All mentioned SNPs are located within the major histocompatibility complex (MHC) region of chromosome 6 and near genes of known immune functions or associations with other autoimmune diseases such as HLA-DQ alpha chain 1 (HLADQA1), MICA, MICB, MSH5, TRIM39, and RPP21. E.g., TRIM39-RPP21 read through transcript is known mediator of the interferon response, a central pathway involved in the pathogenesis of CLE and systemic lupus erythematosus (SLE). Taken together, this genome-wide analysis of disease-association of CLE identified candidate genes and genomic regions that may contribute to pathogenic mechanisms in CLE via dysregulated antigen presentation (HLADQA1), apoptosis regulation, RNA processing and interferon response (MICA, MICB, MSH5, TRIM39, RPP21). This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Experimental Dermatology 04/2015; DOI:10.1111/exd.12708 · 4.12 Impact Factor
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    ABSTRACT: Lymphatic invasion and accumulation of continuous collagen bundles around tumor cells are associated with poor melanoma prognosis, but the underlying mechanisms and molecular determinants have remained unclear. We show here that a copy number gain or overexpression of the membrane-type matrix metalloproteinase MMP16 is associated with poor clinical outcome, collagen bundle assembly around tumor cell nests, and lymphatic invasion. In cultured WM852 melanoma cells derived from human melanoma metastasis, silencing of MMP16 resulted in cell-surface accumulation of the MMP16 substrate MMP14 as well as L1CAM cell adhesion molecule, identified here as a novel MMP16 substrate. When limiting the activities of these transmembrane protein-substrates towards pericellular collagen degradation, cell junction disassembly, and blood endothelial transmigration, MMP16 supported nodular-type growth of adhesive collagen-surrounded melanoma cell nests, coincidentally steering cell collectives into lymphatic vessels. These results uncover a novel mechanism in melanoma pathogenesis, whereby restricted collagen infiltration and limited mesenchymal invasion are unexpectedly associated with the properties of the most aggressive tumors, revealing MMP16 as a putative indicator of adverse melanoma prognosis. Copyright © 2015, American Association for Cancer Research.
    Cancer Research 03/2015; 75(10). DOI:10.1158/0008-5472.CAN-14-1923 · 9.28 Impact Factor
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    ABSTRACT: Gastrointestinal dysfunction is a disabling manifestation of APECED possibly related to an autoimmune intestinal aggression. We evaluated its features in a cohort of 31 Finnish patients. The most frequent manifestations were constipation (48%), diarrhea, dysphagia and retrosternal pain (45%). AADC and TPH-1 autoantibodies were detected in 51 % and 45% of the patients, respectively. Forty-three percent displayed a T-cell response to AADC. One third of the patients also had AIE-75 (33%) and villin (29%)-specific autoantibodies while antibodies against brush borders and Paneth cells were detected in 29% and 20%, respectively. Intestinal IL-17 expression was absent/decreased in 77% of the cases. Duodenal CgA and serotonin expression was absent/decreased in 50% and 66% of the patients, respectively. Constipation correlated with lacking serotonin expression and AADC antibodies (p < 0.05). Copyright © 2015. Published by Elsevier Inc.
    Clinical Immunology 03/2015; 158(2). DOI:10.1016/j.clim.2015.03.012 · 3.99 Impact Factor
  • Journal of the American Academy of Dermatology 02/2015; 72(2):352-3. DOI:10.1016/j.jaad.2014.10.017 · 5.00 Impact Factor
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    ABSTRACT: Subcutaneous panniculitis-like T cell lymphomas represent a rare and difficult to diagnose entity of cutaneous T cell lymphomas. SPTL affects predominantly young adults and presents with multifocal subcutaneous nodules and frequently associated autoimmune features. The pathogenesis of SPTL is not completely understood. The aim of this study was to unravel molecular pathways critical to the SPTL pathogenesis. Therefore, we analyzed 23 skin samples from 20 newly diagnosed SPTL patients and relevant control samples of adipose and non-malignant panniculitis tissue by using gene expression microarray, quantitative PCR, and two-colour immunohistochemistry. Interestingly, indoleamine 2,3-dioxygenase (IDO-1), an immunotolerance-inducing enzyme, was among the most highly overexpressed genes in all comparisons. The expression of Th1-specific cytokines, known to be associated with autoimmune inflammation (i.e. IFNG, CXCR3, CXCL9, CXCL10, CXCL11, and CCL5), were also significantly increased. Confirmed using immunohistochemistry, the morphologically malignant lymphocytes expressed CXCR3 and CXCL9. IDO-1 expression was found both in some morphologically malignant lymphocytes rimming the adipocytes and in surrounding CD11c(-) CD68(-) cells but not in CD11c(+) dendritic cells in the microenvironment. The proportion of FoxP3+ cells in SPTL exceeded that in the benign panniculitis samples. Our results indicate that the up regulation of the tolerogenic IDO-1 together with the up regulation of IFNG, CXCR3 ligands, and CCL5 are features of SPTL lesions. We anticipate that the IFNG-inducible IDO-1 expression contributes to the formation of an immunosuppressive microenvironment, favorable for the malignant T cells. This study provides a relevant molecular basis for further studies exploring novel therapeutic means for subcutaneous T cell lymphoma.
    Orphanet Journal of Rare Diseases 12/2014; 9(1). DOI:10.1186/s13023-014-0160-2 · 3.96 Impact Factor
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    ABSTRACT: Lentigo maligna (LM) is an in situ form of melanoma which can progress into invasive lentigo maligna melanoma (LMM). Variations in the pigmentation and thus visibility of the tumour make assessment of lesion borders challenging. We tested hyperspectral imaging system (HIS) in in vivo preoperative delineation of LM and LMM margins. We compared lesion margins delineated by HIS with those estimated clinically, and confirmed histologically. A total of 14 LMs and 5 LMMs in 19 patients were included. HIS analysis matched the histo-pathological analysis in 18/19 (94.7%) cases while in 1/19 (5.3%) cases HIS showed lesion extension not confirmed by histopathology (false positives). Compared to clinical examination, HIS defined lesion borders more accurately in 10/19 (52.6%) of cases (wider, n = 7 or smaller, n = 3) while in 8/19 (42.1%) cases lesion borders were the same as delineated clinically as confirmed histologically. Thus, HIS is useful for the detection of subclinical LM/LMM borders.
    British Journal of Dermatology 11/2014; 95(5). DOI:10.2340/00015555-2010 · 4.10 Impact Factor
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    ABSTRACT: To assess the predictive value of skin prick testing in early childhood on subsequent allergic symptoms up to adult age. A cohort of 200 unselected healthy newborns was prospectively followed from birth to 20 years of age. Of them, 163 (82%) were reassessed at age 5 years, 150 (76%) at age 11 years, and 164 (83%) at age 20 years with a skin prick test that included 11 common allergens. On the basis of clinical examination and structured interview, the occurrence of atopic dermatitis, allergic rhinoconjunctivitis, recurrent wheezing, and symptoms of food hypersensitivity were recorded at each of the follow-up visits. The reproducibility of skin prick test positivity at age 5 years was 100% at ages 11 and 20 years, ie, none of the skin prick-positive subjects turned negative during the follow-up. Gaining of new sensitizations to aeroallergens was common. Skin prick test positivity at age 5 years predicted allergic symptoms at ages 11 (sensitivity 28%, specificity 94%) and 20 years (sensitivity 23%, specificity 91%) but not atopic dermatitis. Skin prick test positivity at age 5 years strongly predicts later skin prick test positivity and is associated with respiratory symptoms, ie, allergic rhinoconjunctivitis and recurrent wheezing, at ages 11 and 20 years. However, skin prick test negativity at age 5 years does not exclude sensitization and allergic symptoms at a later age. Copyright © 2014 Elsevier Inc. All rights reserved.
    Journal of Pediatrics 11/2014; 166(2). DOI:10.1016/j.jpeds.2014.10.009 · 3.74 Impact Factor
  • 2nd Annual Meeting of the International-Cytokine-and-Interferon-Society; 11/2014
  • Journal of Allergy and Clinical Immunology 08/2014; 134(4). DOI:10.1016/j.jaci.2014.07.008 · 11.25 Impact Factor
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    ABSTRACT: Abstract Autoimmune polyendocrinopathy - candidiasis - ectodermal dystrophy (APECED) is caused by mutations in the Autoimmune regulator (AIRE) gene and is associated with neutralizing anti-cytokine autoantibodies. We have used an in vivo challenge model to analyze antigen-specific CD4(+) T cell responses. Bacille Calmette-Guérin (BCG)-vaccinated patients and controls were injected tuberculin intradermally, skin blisters were induced by suction on the indurations and on unexposed skin, and the infiltrating cells harvested. The patients had a quantitatively normal CD4(+) T cell response and no significant abnormalities in the expression of T helper type (Th) 1- or Th2-related genes. The expression of interleukin (IL)-22, in contrast, was lower in the patients. Two patients, both with a pre-existing ocular keratopathy, experienced a relapse of keratoconjunctivitis, suggesting a possible immunological basis for this APECED component. Our in vivo data are compatible with a selective IL-22 defect in the activated CD4(+) T cells of APECED patients, affecting also unexposed skin in steady-state conditions.
    Autoimmunity 06/2014; DOI:10.3109/08916934.2014.929666 · 2.75 Impact Factor
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    ABSTRACT: Objective Patients with Addison's disease (AD) on conventional replacement therapy have impaired health-related quality of life (HRQoL). It is possible that lower hydrocortisone (HC) doses recommended by current guidelines could restore HRQoL. We compared HRQoL in AD patients treated according to current HC recommendations to that of the age- and gender-standardized general population.Subjects, design and measurementWe assessed HRQoL in a cross-sectional setting with the 15D instrument in a Finnish AD cohort (n=107) and compared the results with those of a large sample of general population (n= 5671).We examined possible predictors of HRQoL in AD. Within the patient group, HRQoL was also assessed by SF-36.ResultsMean HC dose was 22 mg/d, corresponding to 12 ± 4mg/m2. HRQoL was impaired in AD compared to the general population (15D score; 0.853 vs. 0.918, p<0.001). Within single 15D dimensions, discomfort and symptoms, vitality and sexual activity were most affected. Stepwise regression analysis demonstrated that Patient's Association membership (p = 0.02), female gender (p<0.01), presence of other autoimmune or inflammatory co-morbidity (p <0.02), lower education (p < 0.02), and longer disease duration (p <0.05), independently predicted impaired HRQoL. Replacement regimens, autoimmune-related co-morbidities, total number of co-morbidities or level of healthcare follow-up did not. In AD, HRQoL was impaired also as assessed by SF-36.ConclusionsHRQoL is significantly impaired in AD compared to the general population despite use of recommended HC doses. Patient's Association membership was the most significant predictor of impaired HRQoL. This finding should be explored in more detail in the future.This article is protected by copyright. All rights reserved.
    Clinical Endocrinology 05/2014; 81(4). DOI:10.1111/cen.12484 · 3.35 Impact Factor
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    ABSTRACT: Autoimmune tubulo-interstitial nephritis (TIN) is a rare complication of autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED). Previous data on TIN and other renal or urologic manifestations of APECED are sparse. We performed a retrospective study on the urinary and renal tract diseases in a cohort of 30 Finnish patients with APECED (mean age 40 years), with special emphasis on the clinical presentation and the immunologic characteristics of TIN. Clinical and laboratory findings, specific anticytokine and kidney-specific antibodies were analysed. Five of the 30 (17%) patients had moderate-to-severe renal failure, including 3 (10%) with TIN, leading to either transplantation, haemodialysis or immunosuppressive treatment. No other cause other than APECED was found for the TIN. All three patients with TIN had circulating antibodies against the distal part of the nephron, as did 30% of all cohort cases. Two had nephrocalcinosis, and two had renal tubular acidosis type 1. Immunosuppressive therapy with mycophenolate mofetil or rituximab in one pediatric case did not revert the TIN, however. Renal failure should raise concern for TIN in APECED. It discloses some specific features: no uveitis, no glycosuria and inconstant urinalysis anomalies. Regular renal monitoring for any APECED patient should be performed. Circulating antibodies against the distal part of the nephron are frequent and present in all TIN patients, but their pathologic significance is not yet known. Future studies will be needed to understand the triggers leading to overt clinical disease in these patients.
    Nephrology Dialysis Transplantation 04/2014; 29(9). DOI:10.1093/ndt/gfu064 · 3.49 Impact Factor
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    ABSTRACT: Sezary syndrome (SS) is an aggressive leukemic variant of cutaneous T-cell lymphoma. Recurrent chromosomal aberrations have been found in SS, but the whole genetic mutation spectrum is unknown. To better understand the molecular pathogenesis of SS, we performed exome sequencing, copy number variation (CNV) and gene expression analysis of primary SS cells. In our index patient with typical SS, we found novel somatic missense mutations in TBL1XR1, EPHA7 and SLFN12 genes in addition to larger chromosomal changes. The mutations are located in biologically relevant genes affecting apoptosis and T-cell maturation. They may play a role in the pathobiology of the disease, but no recurrent mutations were discovered in nine additional SS patients studied. Thus, screening of larger patient cohorts are needed to confirm their prevalence and biological significance in SS. This article is protected by copyright. All rights reserved.
    Experimental Dermatology 04/2014; 23(5). DOI:10.1111/exd.12405 · 4.12 Impact Factor
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    ABSTRACT: After the first investigational study on the use of extracorporeal photopheresis for the treatment of cutaneous T-cell lymphoma was published in 1983 with its subsequent recognition by the FDA for its refractory forms, the technology has shown significant promise in the treatment of other severe and refractory conditions in a multi-disciplinary setting. Among the major studied conditions are graft versus host disease after allogeneic bone marrow transplantation, systemic sclerosis, solid organ transplant rejection and inflammatory bowel disease. In order to provide recognized expert practical guidelines for the use of this technology for all indications the European Dermatology Forum (EDF) proceeded to address these questions in the hands of the recognized experts within and outside the field of dermatology. This was done using the recognized and approved guidelines of EDF for this task. These guidelines provide at present the most comprehensive available expert recommendations for the use of extracorporeal photopheresis based on the available published literature and expert consensus opinion.
    Journal of the European Academy of Dermatology and Venereology 01/2014; 28 Suppl 1:1-37. DOI:10.1111/jdv.12311 · 3.11 Impact Factor
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    ABSTRACT: Context: Previous studies have identified the calcium-sensing receptor (CaSR) and NALP5 as parathyroid autoantibody targets in patients with autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED). However, although NALP5 antibodies have been associated with the occurrence of hypoparathyroidism (HP) in APECED, it is unclear whether or not CaSR antibodies are a specific or sensitive marker for APECED-associated HP. Objective: To identify associations between the presence of CaSR and NALP5 antibodies and the disease manifestations and demographic characteristics of Finnish APECED patients. Design, Subjects and Methods: This was a case-control study including 44 APECED patients and 38 age- and sex-matched healthy controls. Antibodies against the CaSR and NALP5 were detected using immunoprecipitation assays and radioligand binding assays, respectively. Results: CaSR and NALP5 antibodies were detected in 16 out of 44 (36%) and 13 out of 44 (30%) patients, respectively. No statistically significant associations were found between the presence of CaSR nor NALP5 antibodies and the disease manifestations of APECED including HP (P values were > 0.05). For the diagnosis of HP, CaSR and NALP5 antibodies had specificities of 83% and 50%, respectively, and sensitivities of 39% and 26%, respectively. A significant association between both a shorter APECED and HP duration (< 10 years) and positivity for CaSR antibodies was noted (P = 0.019 and 0.0061, respectively). Conclusion: Neither CaSR nor NALP5 antibodies were found to be specific or sensitive markers for HP in APECED. Further investigations are required to determine the exact role of the autoimmune response against the CaSR and NALP5 in the pathogenesis of this autoimmune syndrome.
    The Journal of Clinical Endocrinology and Metabolism 12/2013; 99(3):jc20133723. DOI:10.1210/jc.2013-3723 · 6.31 Impact Factor
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    ABSTRACT: Background Hypersensitivity to raw fruits and vegetables is often associated with respiratory allergy to birch (Betula verrucosa) pollen and is considered to be the most prevalent form of food allergy in adults sensitized to birch pollen. Objective The aim of the study was to investigate the association of clinical allergy and IgE profiles in individuals with birch pollen allergy and hypersensitivity to raw fruits and vegetables. Methods A total of 59 adults with clinical and skin prick test confirmed birch pollen allergy were included in the study. All the subjects were interviewed by using a structured questionnaire and were examined in vivo by the open test, with the appropriate fruits and vegetables. ImmunoCAP and ImmunoCAP ISAC were used as in vitro diagnostics to assess sensitization profiles for each individual, and principal components analysis was used to analyze the IgE data sets. Results Of 59 individuals, 54 (92%) had positive prick-prick test with raw potato, carrot, apple, and/or hazelnut, and the skin prick test was always positive when the corresponding skin challenge was defined as positive. Specific IgE in the ImmunoCAP and inhibition assays with rMal d 1 and rBet v 1 demonstrated that Bet v 1 is driving the sensitization against pathogenesis related-10 proteins. However, positive IgE in vitro results could not be used to predict clinical reactivity to raw fruits and vegetables. Conclusions The present study showed that component-based IgE profiling does not enhance the diagnostic potential in case of pollen-food syndrome, which may be associated with other as yet unidentified components.
    11/2013; 1(6):623–631.e1. DOI:10.1016/j.jaip.2013.07.010
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    ABSTRACT: The pathomechanism of mycosis fungoides (MF), the most common type of primary cutaneous T-cell lymphomas (CTCLs) and a malignancy of non-recirculating, skin-resident T-cells, is unknown albeit underlying viral infections have been sought for. Human endogenous retroviruses (HERVs) are ancient retroviral sequences in the human genome and their transcription is often deregulated in cancers. We explored the transcriptional activity of HERV sequences in a total of 34 samples comprising MF and psoriasis skin lesions, as well as corresponding non-malignant skin using a retrovirus-specific microarray and quantitative RT-PCR. To identify active HERV-W loci, we cloned the HERV-W specific RT-PCR products, sequenced the cDNA clones and assigned the sequences to HERV-W loci. Finally, we used immunohistochemistry on MF patient and non-malignant inflammatory skin samples to confirm specific HERV-encoded protein expression. Firstly, a distinct, skin-specific transcription profile consisting of five constitutively active HERV groups was established. Although individual variability was common, HERV-W showed significantly increased transcription in MF lesions compared to clinically intact skin from the same patient. Predominantly transcribed HERV-W loci were found to be located in chromosomes 6q21 and 7q21.2, chromosomal regions typically altered in CTCL. Surprisingly, we also found the expression of 7q21.2/ERVWE1-encoded Syncytin-1 (Env) protein in MF biopsies and expression of Syncytin-1 was seen in malignant lymphocytes, especially in the epidermotropic ones, in 15 of 30 cases studied. Most importantly, no Syncytin-1 expression was detected in inflammatory dermatosis (Lichen ruber planus) with skin-homing, non-malignant T lymphocytes. The expression of ERVWE1 mRNA was further confirmed in 3/7 MF lesions analyzed. Our observations strengthen the association between activated HERVs and cancer. The study offers a new perspective into the pathogenesis of CTCL since we demonstrate that differences in HERV-W transcription levels between lesional MF and non-malignant skin are significant, and that ERVWE1-encoded Syncytin-1 is expressed in MF lymphoma cells.
    PLoS ONE 10/2013; 8(10):e76281. DOI:10.1371/journal.pone.0076281 · 3.53 Impact Factor

Publication Stats

6k Citations
1,157.05 Total Impact Points

Institutions

  • 1981–2015
    • Helsinki University Central Hospital
      • • Division of Allergology
      • • Division of Dermatology and Venereology
      • • Skin and Allergy Hospital
      Helsinki, Uusimaa, Finland
  • 1976–2015
    • University of Helsinki
      • • Skin and Allergy Hospital
      • • Department of Dermatology
      • • Institute of Clinical Medicine
      • • Fourth Department of Medicine
      • • Transplantation Laboratory
      Helsinki, Uusimaa, Finland
  • 2006
    • Hospital District for Helsinki and Uusimaa
      Helsinki, Southern Finland Province, Finland
  • 2000
    • Fundación Jiménez Díaz
      Madrid, Madrid, Spain
  • 1997–1999
    • Tampere University Hospital (TAUH)
      Tammerfors, Pirkanmaa, Finland
  • 1998
    • Oulu University Hospital
      • Department of Dermatology
      Uleoborg, Northern Ostrobothnia, Finland
  • 1985–1996
    • University of Tampere
      • Institute of Biomedical Sciences
      Tammerfors, Pirkanmaa, Finland
  • 1995
    • University of Oulu
      Uleoborg, Northern Ostrobothnia, Finland
  • 1992
    • University of Kuopio
      Kuopio, Northern Savo, Finland
  • 1988
    • National Cancer Institute (USA)
      • Laboratory of Cell Biology
      Maryland, United States
  • 1987
    • National Institutes of Health
      • Laboratory of Cell Biology
      Maryland, United States