Hye Sung Kim

Catholic University of Korea, Sŏul, Seoul, South Korea

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Publications (47)129.81 Total impact

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    ABSTRACT: Gold nanoparticles (AuNP) and quantum dots (Qdot) were clicked into doxorubicin nanoclusters that showed enzyme-dependent dissociation behaviors for differential cellular uptakes and imaging. The AuNP were co-functionalized with doxorubicin (DOX) and azide-terminated polymer (DOX/azide@AuNP), while an enzyme-cleavable peptide and alkyne-terminated polymer were sequentially conjugated on Qdot surface (Alkyne-MMP@Qdot). Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, and fluorescence imaging detected the azide and alkyne groups on DOX/azide@AuNP and Alkyne-MMP@Qdot, respectively, and the click-reactivity was also confirmed. In the presence of the catalyst, two nanoparticles were clicked to doxorubicin nanoclusters, which increased the volume of the particles ca. 343-fold within 30min. Upon matrix metalloproteinase-2 (MMP-2) digestion, the nanoclusters were clearly dissociated into smaller particles, and the fluorescence of the quenched Qdot was also recovered, which suggests that the nanoclusters respond to MMP-2 concentrations and can thus be employed for cancer imaging. Confocal microscopy and an elemental analysis of the cancer cells revealed that the cellular uptakes of doxorubicin nanoclusters significantly increased at higher MMP-2 concentrations, and doxorubicin could also be cleaved for anti-cancer effects. In vivo and in vitro cytotoxicity assay accordingly showed that the cytotoxicity of doxorubicin nanoclusters against cancer cells increased in MMP-2-rich environments such as tumor site. Thus, these nanoclusters containing DOX/azide@AuNP and Alkyne-MMP@Qdot are expected to be multifunctional carriers for targeted anti-cancer treatments and imaging. Copyright © 2015. Published by Elsevier B.V.
    Journal of Controlled Release 08/2015; 217. DOI:10.1016/j.jconrel.2015.08.037 · 7.71 Impact Factor
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    Hye Sung Kim · Hyuk Sang Yoo ·
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    ABSTRACT: Focal adhesion between substrates and cells greatly controls cytoskeletal changes as well as differentiation of the cultivating cells. We here investigated focal adhesion behaviors and differentiation profiles of adipose-derived stem cells on the nano-scaled pillar arrays with diverse pillar-to-pillar distances.
    RSC Advances 06/2015; 5(61):49508-49512. DOI:10.1039/C5RA07608K · 3.84 Impact Factor
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    ABSTRACT: Bacteria cellulose membranes (BCM) are used for wound dressings, bone grafts, tissue engineering, artificial vessels, and dental implants because of their high tensile strength, crystallinity and water holding ability. In this study, the effects of BCM application for 15 days on healing of burn wounds were investigated based on evaluation of skin regeneration and angiogenesis in burn injury skin of Sprague-Dawley (SD) rats. BCM showed a randomly organized fibrils network, 12.13MPa tensile strength, 12.53% strain, 17.63% crystallinity, 90.2% gel fraction and 112.14g×m(2)/h highest water vapor transmission rate (WVTR) although their swelling ratio was enhanced to 350% within 24h. In SD rats with burned skin, the skin severity score was lower in the BCM treated group than the gauze (GZ) group at all time points, while the epidermis and dermis thickness and number of blood vessels was greater in the BCM treated group. Furthermore, a significant decrease in the number of infiltrated mast cells and in vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) expression was observed in the BCM treated group at day 10 and 15. Moreover, a significant high level in collagen expression was observed in the BCM treated group at day 5 compared with GZ treated group, while low level was detected in the same group at day 10 and 15. However, the level of metabolic enzymes representing liver and kidney toxicity in the serum of BCM treated rats was maintained at levels consistent with GZ treated rats. Overall, BCM may accelerate the process of wound healing in burn injury skin of SD rats through regulation of angiogenesis and connective tissue formation as well as not induce any specific toxicity against the liver and kidney. Copyright © 2014 Elsevier Ltd. All rights reserved.
    05/2015; 122:387-98. DOI:10.1016/j.carbpol.2014.10.049
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    ABSTRACT: Patients with atopic dermatitis (AD) should be relatively well informed about the disorder to control their condition and prevent flare-ups. Thus far, there is no accurate information about the disease awareness levels and therapeutic behavior of AD patients. To collect data on patients' knowledge about AD and their behavior in relation to seeking information about the disease and its treatment. We performed a questionnaire survey on the disease awareness and self-management behavior of AD patients. A total of 313 patients and parents of patients with AD who had visited the The Catholic University of Korea, Catholic Medical Center between November 2011 and October 2012 were recruited. We compared the percentage of correct answers from all collected questionnaires according to the demographic and disease characteristics of the patients. Although dermatologists were the most frequent disease information sources and treatment providers for the AD patients, a significant proportion of participants obtained information from the Internet, which carries a huge amount of false medical information. A considerable number of participants perceived false online information as genuine, especially concerning complementary and alternative medicine treatments of AD, and the adverse effects of steroids. Some questions on AD knowledge had significantly different answers according to sex, marriage status, educational level, type of residence and living area, disease duration, disease severity, and treatment history with dermatologists. Dermatologists should pay more attention to correcting the common misunderstandings about AD to reduce unnecessary social/economic losses and improve treatment compliance.
    Annals of Dermatology 02/2015; 27(1):40-7. DOI:10.5021/ad.2015.27.1.40 · 1.39 Impact Factor
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    ABSTRACT: The microstructure and characteristics of silicon-incorporated diamond-like carbon film, fabricated using a radio-frequency plasma-enhanced chemical vapor deposition process with hexamethyldisilane [(CH3)3Si·Si(CH3)3:HMDS] gas as a silicon source, were investigated. Diamond-like carbon films with silicon compositions from 0 to 5 atomic percent were deposited onto ultra-fine grained AZ31 magnesium alloy substrate as buffer layers or multilayers. Si doping led not only to an increase in the bonding ratio (sp3/sp2), but improvements in hardness, critical adhesion, and corrosion resistance. Out of the investigated samples, the multi-deposited silicon diamond-like carbon thin film on magnesium substrate showed the best combination of adhesive, wear resistance, and corrosion resistance properties.
    Journal of Nanoscience and Nanotechnology 12/2014; 14(12):9124-30. DOI:10.1166/jnn.2014.10101 · 1.56 Impact Factor
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    ABSTRACT: This study aimed to check the status of the contact investigation in congregate settings to eradicate tuberculosis (TB) in the Republic of Korea. The "Integrated System for Disease and Public Health Management" is used for care and follow-up for patients and contacts of TB. We downloaded data for contact investigations conducted from January to December 2013. A total of 1,200 contact investigations in congregate settings were carried out by 25 field investigators in 2013. We performed the status of contact investigation, TB, and LTBI rate by age, accept rate of LTBI treatment, and complete rate of LTBI treatment during 2013. A total of 1,547 index TB patients, 149,166 contacts, and 259 additional TB patients were found through the investigation. Kindergartens showed the highest LTBI rate, 19.8%, among educational facilities. The second highest was in elementary schools and the subtotal LTBI rate of educational facilities was 7.8%. Social welfare/correctional facilities and workplaces showed relatively high LTBI rates of 23.8% and 23.6%, respectively. By age, individuals >35 years showed the highest LTBI rate, followed by those aged 0-4 years, 30-34 years, and 5-9 years, with rates of 18.1%, 16.4%, and 15.4% respectively. When comparing the tuberculin skin test (TST) positive conversion ratio by facility, middle school and high school were relatively high compared to the others. The accept rate of LTBI treatment in the workplace was lowest at 63% and the complete rate in elementary schools was lowest at 76.5%. TB contact investigation is considered as a meaningful strategy for preventing TB outbreaks in congregate settings and decreasing the prevalence of TB in young people. Results of this study could be used to establish the LTBI management policy.
    12/2014; 5(Suppl):S30-S36. DOI:10.1016/j.phrp.2014.10.010
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    ABSTRACT: Microstructural changes and corrosion behavior of pure magnesium for different milling times were investigated. The samples with a finer grain size showed poor corrosion resistance because of unstable or metastable protective film formation after immersion in 0.8 wt% NaCl solution. The corrosion resistance did not improve despite the strong (0002) texture of the sample prepared by spark plasma sintering at 500 degrees C for 0.3 Ks and milling for 2 h. By studying the microstructural changes and texture development, we concluded that the deformation-dependent grain size is the dominant factor controlling the corrosion properties of mechanically milled magnesium. Increased grain boundary densities lead to an enhancement of the overall surface reactivity and, consequently, the corrosion rate.
    Metals and Materials International 11/2014; 20(6):1095-1101. DOI:10.1007/s12540-014-6023-5 · 1.58 Impact Factor
  • Hye Sung Kim · Hyuk Sang Yoo ·

    Journal of Controlled Release 09/2014; 190:64-6. DOI:10.1016/j.jconrel.2014.07.031 · 7.71 Impact Factor
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    ABSTRACT: Background Idiopathic interstitial pneumonia (IIP) is characterized by chronic interstitial inflammation and fibrosis. Although mounting evidence has suggested that toll-like receptor (TLR) 2 and TLR4 are involved in the pathogenesis of non-infectious lung injury in vitro and in mouse models, their roles in human IIP remain unknown. Methods To address this issue, we investigated the expression patterns of TLR2 and TLR4 by immunohistochemistry in resected lung tissues from patients with usual interstitial pneumonia (UIP) or nonspecific interstitial pneumonia (NSIP). Results Type II pneumocytes, bronchial epithelial cells (BECs), and alveolar macrophages accounted for the majority of TLR2- and TLR4-expressing cells in both UIP and NSIP. The numbers of TLR2 and TLR4-positive respiratory epithelial (RE) cells, including type II pneumocytes and BECs, were significantly greater in UIP and NSIP than in the control. In particular, the numbers of TLR2-positive RE cells were much greater in UIP than in NSIP. The intensities of TLR2 and TLR4 expression in type II pneumocytes were also significantly stronger in UIP and NSIP than in the control. A comparison of the TLR expression patterns between the fibroblastic and fibrotic areas in UIP indicated that the numbers TLR2 and TLR4-positive RE cells were similar in fibroblastic areas, whereas the TLR2-positive RE cells outnumbered the TLR4-positive RE cells in the fibrotic areas. Conclusions This study demonstrates that RE cells over-express TLR2 and TLR4 in the lungs of IIP patients. These findings suggest that high expression of TLRs may contribute to the pathogenesis of human IIP.
    Respiratory medicine 05/2014; 108(5). DOI:10.1016/j.rmed.2013.12.007 · 3.09 Impact Factor
  • Hye Sung Kim · Hyuk Sang Yoo ·
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    ABSTRACT: Fabricating tissue architecture-mimicking scaffolds is one of the major challenges in the field of tissue engineering. Electrospun nanofibers have been considered as potent techniques for fabricating fibrous scaffolds biomimicking extracellular frameworks. Therapeutic agent-incorporated nanofibrous meshes have widely served as excellent substrates for adhesion, proliferation and differentiation. Many drugs, proteins and nucleic acids were incorporated into the scaffolds for regeneration of skin, musculoskeletal, neural and vascular tissue engineering in aims to control the release of the therapeutic agents. In the current article, we focus on introducing various fabrication techniques for electrospun nanofiber-based scaffolds and subsequent functionalization of nanofibers for therapeutic purposes. We also detail how the therapeutic nanofibrous meshes can be employed in the field of tissue engineering.
    Nanomedicine 04/2014; 9(4):517-533. DOI:10.2217/nnm.13.224 · 5.41 Impact Factor
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    ABSTRACT: This study was carried out to investigate the residual characteristics and calculate processing factor of the environment friendly material matrine in fresh chilli pepper by drying. Spray solution of matrine was prepared by dilution of the commercial product (2% active ingredient) with water at 1 : 1000 (v/v) ratio and sprayed onto chilli pepper plants at seven day intervals. Samples were collected at 0, 1, 3, 5 and 7 days after last application and then dried using a hot air dry oven at for 36 hours until the water content was reduced to 14%. Recoveries and storage period stabilities of matrine in the samples ranged from 106.6 to 119.1% and 106.6 to 113.1%, respectively. The residual concentrations of matrine in fresh chilli pepper and dried chilli peppers treated only once were found to be from less than 0.01 to 0.11 and from 0.03 to 0.25 mg/kg, respectively. In case of plants sprayed twice with matrine, the residual concentrations ranged from 0.02 to 0.12 and from 0.04 to 0.4 mg/kg, respectively. Processing factor of matrine in the fresh chilli pepper by drying was found to be from 1.5 to 3.3, indicating that the residual concentration of matrine in dried chilli pepper increased about two or three times by drying.
    12/2013; 17(4). DOI:10.7585/kjps.2013.17.4.244
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    Byung Ju Song · Ka Ram Kim · Hye Sung Kim ·
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    ABSTRACT: Microstructural and mechanical properties of Ni-YSZ fabricated using SPS processing have been investigated at various sintering temperatures. Our study shows samples to be applied as a SOFC anode have the proper porosity of 40% and high hardness when processed at . These results are comparable to the values obtained at higher sintering temperature reported by others. This result is important because when the fabrication processes are performed above , the mechanical property starts to decrease drastically. This is caused by the fast grain coarsening at the higher temperature, which initiates a mismatch between thermal expansion coefficients of Ni and YSZ and induces cracks as well.
    12/2013; 20(6). DOI:10.4150/KPMI.2013.20.6.425
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    The Journal of Dermatology 12/2013; 41(1). DOI:10.1111/1346-8138.12320 · 2.25 Impact Factor
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    ABSTRACT: Breast cancer anti-estrogen resistance 3 (BCAR3) is an SH2-containing signal transducer and is implicated in tumorigenesis of breast cancer cells. In this study, we found that BCAR3 mediates the induction of ERK activation and DNA synthesis by insulin, but not by IGF-1. Specifically, the SH2 domain of BCAR3 is involved in insulin-stimulated DNA synthesis. Differential tyrosine-phosphorylated patterns of the BCAR3 immune complex were detected in insulin and IGF-1 signaling, suggesting that BCAR3 is a distinct target molecule of insulin and IGF-1 signaling. Moreover, microinjection of BCAR3 inhibitory materials inhibited membrane ruffling induced by insulin, while this did not affect insulin-mediated GLUT4 translocation. Taken together, these results demonstrated that BCAR3 plays an important role in the signaling pathways of insulin leading to cell cycle progression and cytoskeleton reorganization, but not GLUT4 translocation.
    Biochemical and Biophysical Research Communications 11/2013; 441(4). DOI:10.1016/j.bbrc.2013.10.161 · 2.30 Impact Factor
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    ABSTRACT: We investigated the electrical conductivity (sigma) and mechanical property of polyvinylchloride/carbon nanotube composites as a function of the CNT content and processing time during a solid-state process of high speed vibration mixing (HSVM) and high energy ball milling (HEBM). Both processes were suggested to avoid high temperatures, solvents, chemical modification of carbon nanotubes. In this study, the percolation threshold (phi(c)) for electrical conduction is about 1 wt% CNT with a sigma value of 0.21 S/m, and the electrical conductivity is higher value than that reported by other researchers from melt mixing process or obtained from the other solid-state processes. We found that the dispersion of CNTs and morphology change from CNT breaking are closely related to sigma. Especially, a large morphology change in the CNTs was occurred at the specific processing time, and a significant decrease in the electrical conductivity of polyvinylchloride/carbon nanotube composite occurred in this condition. A meaningful increase of electrical properties and mechanical property is observed in the sample with about 1-2 wt% CNT contents sintered at 200 degrees C after the milling for 20 min by HEBM process. Our study indicates the proper process condition required to improve sigma of PVC/CNT composites.
    Journal of Nanoscience and Nanotechnology 11/2013; 13(11):7723-7. DOI:10.1166/jnn.2013.7830 · 1.56 Impact Factor
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    ABSTRACT: Cheonggukjang (CKJ), a fermented soybean product, has been reported to have beneficial effects on various chronic diseases, including cardiovascular disease, cancer and immune diseases. To investigate whether CKJ induces growth sensitivity in mammals, alterations of key parameters related to their growth were analyzed. Sprague‑Dawley (SD) rats were treated with a high concentration of CKJ (H‑CKJ) or a low concentration of CKJ (L‑CKJ) for 10 days, and compared with vehicle-treated rats. The CKJ contained a high concentration of total flavonoids, phenolic compounds, daidzein and genistein, compared with the non-fermented soybean product. Body weight was higher in the H‑CKJ‑treated group compared with that in the vehicle‑ and L‑CKJ‑treated groups, whereas the weights of three organs (the brain, liver and kidney) were higher in the L‑CKJ‑treated group compared with the remaining two groups. However, no significant differences in femur length and weight were detected between the CKJ‑ and vehicle‑treated groups. The thickness of the epiphyseal growth plate in proximal femoral epiphysis was broadest in the H‑CKJ‑treated group compared with the vehicle- and L‑CKJ‑treated groups. Furthermore, the level of growth hormone (GH) was highest in the serum of the L‑CKJ‑treated group, although that of the H‑CKJ‑treated group was lower compared with that in the L‑CKJ group. Moreover, the expression levels of the GH receptor increased in the liver tissue, but not in the muscle tissue, of the L‑CKJ‑ and H‑CKJ‑treated groups. In the downstream signaling pathway of the GH receptor, the phosphorylation levels of Akt and Erk were differentially regulated between the liver and muscle. These results suggest that CKJ extract may enhance the sensitivity of the femur, liver and muscle epiphyseal growth plate in SD rats, through the upregulation of GH secretion.
    Molecular Medicine Reports 10/2013; 9(1). DOI:10.3892/mmr.2013.1757 · 1.55 Impact Factor
  • Hye Sung Kim · Woo Jin Kim ·
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    ABSTRACT: Ultrafine-grained (1.1–1.5 μm) AZ61 alloys containing nanoscaled β-Mg17Al12 phase particles (70–140 nm) were prepared using high-ratio differential speed rolling (HRDSR) and their corrosion behaviours were studied in a 0.1 M NaCl solution. The grain size reduction by HRDSR improved the corrosion resistance by enhancing passivity of the surface film. Post-annealing further increased the corrosion resistance by decreasing dislocation density in matrix. When significant grain growth took place, however, the corrosion resistance was decreased because of the pronounced negative effect of the increase in grain size. Refinement of β phase to nanoscale size decreased the susceptibility to microgalvanic corrosion.
    Corrosion Science 10/2013; 75. DOI:10.1016/j.corsci.2013.05.032 · 4.42 Impact Factor

  • International journal of dermatology 04/2013; 53(2). DOI:10.1111/j.1365-4632.2012.05668.x · 1.31 Impact Factor
  • Ji Suk Choi · Hye Sung Kim · Hyuk Sang Yoo ·
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    ABSTRACT: Electrospun nanofibrous mats have recently been employed as drug reservoirs for their unique features, such as high surface-to-volume ratios and easy fabrication process. We describe herein various methods of fabricating drug- and gene-encapsulated nanofibrous meshes, which can be prepared by electrospinning. The electrospinning process of nanofibrous mats is affected by many parameters, including viscosity and ejection speeds of the polymeric solutions and the electrical potential applied to the system. Both single- and dual-nozzle systems are widely employed in the preparation of electrospun nanofibers encapsulating drugs and genes, which are usually incorporated into the electrospun mats either by physical mixing with polymeric solutions before electrospinning or by physical incorporation after electrospinning. Various strategies have been tailored to maintain the bioactivity of proteins for tissue regeneration before and after electrospinning. Nucleic acids, such as DNA and siRNA, are also incorporated into nanofibrous meshes to enhance tissue regeneration by expressing transgenes or silencing domestic genes in specific tissues. Drug- or gene-incorporated nanofibrous meshes can greatly increase tissue regeneration rates and reduce scar formation in normal and diabetic wounds. Hybrid nanofibers, with multiple cell layers or hydrogels, have also been used to improve wound healing efficiency by increasing cell infiltration.
    Drug Delivery and Translational Research 04/2013; 5(2). DOI:10.1007/s13346-013-0148-9
  • Hye Sung Kim · Hyuk Sang Yoo ·
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    ABSTRACT: Human epidermal growth factor (hEGF) gene therapy was achieved with an electrospun nanofibrous mesh with matrix metalloproteinases (MMPs)-responsiveness to control release of plasmid human epidermal growth factor (phEGF) in diabetic ulcers. For MMPs-responsiveness, linear poly (ethyleneimine) (LPEI) was immobilized on the surface of the nanofiber via a MMPs-cleavable linker. phEGF was electrostatically incorporated to LPEI-immobilized nanofibrous meshes with various charge ratios and phEGF incorporation efficiency was increased with increasing charge ratios. The release of phEGF and LPEI were significantly increased in the presence of MMP-2 due to the enzymatic digestion of MMP-cleavable linkage between the matrix and LPEI. Human dermal fibroblasts with the released fraction showed the highest expression level of human EGF compared to naked phEGF or phEGF/LPEI complexes. Diabetic wounds treated with phEGF-incorporated nanofibrous meshes showed high hEGF expression level and accelerated wound recovery rates without wound contractions for 14 days. Neo-collagen and cytokerain accumulation were significantly increased as well as the expression of the keratinocyte-specific markers at the re-epithelized tissue treated with phEGF NF, which clearly indicates that EGF gene was transfected to dermal cells and this consequently assisted wound recovery without phenotypic changes of the re-epithelized tissues. Thus, phEGF-incorporated nanofibrous mesh is expected to accelerate wound healing process as well as reduce wound contraction during recovery of diabetic ulcers.
    Acta biomaterialia 03/2013; 9(7). DOI:10.1016/j.actbio.2013.03.018 · 6.03 Impact Factor

Publication Stats

251 Citations
129.81 Total Impact Points


  • 2007-2015
    • Catholic University of Korea
      • • Department of Dermatology
      • • Department of Internal Medicine
      • • College of Medicine
      Sŏul, Seoul, South Korea
  • 2006-2015
    • Pusan National University
      • • College of Nanoscience and Nanotechnology
      • • Department of Nanomaterials Engineering
      Tsau-liang-hai, Busan, South Korea
  • 2014
    • Korea Centers for Disease Control and Prevention
      Daiden, Daejeon, South Korea
  • 2010-2014
    • Seoul National University Hospital
      • Department of Pathology
      Sŏul, Seoul, South Korea
  • 2009-2014
    • Kangwon National University
      • • Department of Mechanical and Biomedical Engineering
      • • Department of Forest Biomaterials Engineering
      Shunsen, Gangwon-do, South Korea
  • 2010-2012
    • Seoul National University
      • Department of Biomedical Sciences
      Sŏul, Seoul, South Korea
  • 2011
    • Hongik University
      • Department of Materials Science and Engineering
      Sŏul, Seoul, South Korea