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ABSTRACT: Fibroblast growth factor 21 (FGF-21) is a novel regulator for metabolic syndrome (MetS), diabetes, and obesity. However, no study has been performed on the association of these diseases with FGF-21 gene polymorphism. The aim of the study was to investigate the association of 3' untranslated region (UTR) single-nucleotide polymorphisms (SNPs) in the FGF-21 gene with MetS, obesity, and diabetes in the Han Chinese population. A total of 291 subjects were recruited from the Han Chinese population in Sichuan province. The genotypes of FGF-21 were determined by polymerase chain reaction-restriction fragment length polymorphism. The genotypes were confirmed by sequencing. No polymorphisms were found in rs11665841 (1 of 291) and rs3745706 (2 of 291). We did not find an association between genotype frequencies of SNP rs11665896 and lipid concentration, glucose concentration, or blood pressure. The TG/GG genotype relative to the TT genotype had an age- and sex-adjusted odds ratio of 1.41 for MetS (p=0.149), 1.84 (p=0.016) for obesity (body mass index ≥25 kg/m(2)), and 1.19 (p=0.492) for diabetes. Genetic variation of the 3' UTR of the FGF-21 gene was associated with obesity, however, not with MetS or diabetes.
DNA and cell biology 10/2011; 31(4):547-52. · 2.28 Impact Factor
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ABSTRACT: To determine the serum Urea levels in healthy adults living in urban Chengdu and to identify factors that influence the serum urea levels for the purpose of establishing reference interval.
Serum urea levels were determined in 17 787 healthy adults in urban Chengdu who underwent physical examinations. The characteristics of distribution of serum urea levels in the population and its relationships with liver function, renal function, serum glucose, and serum lipid were analyzed.
Increased Crea, Uric, Glu, and HDL-C levels and decreased Glb level were associated with increased serum urea levels, which was independent of the impact of sex and age. The association between serum urea and Crea, Glu, and HDL-C existed in normal healthy adults. Men had higher urea levels than women. Regardless of gender, serum urea increased with age (with a cut off point at 30, 50, 60 and 70 years).
Serum urea levels in healthy adults living in urban Chengdu vary in different gender and age groups. Serum urea levels are associated with serum Crea, Glu, the HDL-C levels. It is necessary to establish gender and age-specific reference intervals for serum urea.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 07/2010; 41(4):684-7.
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ABSTRACT: To investigate the association of plasma fibroblast growth factor-21 (FGF-21) with abdominal obesity and other metabolic indicators.
Sixty one people with abdominal obesity and 113 healthy controls were recruited. The stature, avoirdupois, waist circumference and blood pressure of the participants were measured. Serum lipid, glucose, insulin and plasma FGF-21 levels of those participants were also determined.
1) The participants with abdominal obesity had significantly higher FGF-21 levels than the healthy controls [(1.91 +/- 0.40) ng/mL vs. (1.75 +/- 0.45) ng/mL, P = 0.020]. 2) Plasma FGF-21 levels were positively associated with BMI (r = 0.24, P = 0.001), waist circumference (r = 0.16, P = 0.033), body fat contents (r = 0.24, P = 0.001), and triglycerides (r = 0.16, P = 0.036) after adjustment for age and sex. The multiple liner regression analysis identified BMI, waist circumference, body fat contents, and triglycerides as factors associated with FGF-21 (P < 0.05). 3) Plasma FGF-21 levels were found to be independently associated with abdominal obesity (OR = 2.413, 95% CI 1.115-5.221, P = 0.025).
FGF-21 is an independent risk factor for abdominal obesity. It is perhaps an important factor in the occurrence and development of abdominal obesity.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 05/2010; 41(3):487-9, 522.
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ABSTRACT: Non-small-cell lung cancer (NSCLC) accounts for more than 85% of all cases of lung cancer. The 5-year survival of patients presenting with advanced stage NSCLC is less than 15%, indicating that additional treatment options are needed. Bevacizumab is a recombinant humanized version of the murine anti-human vascular endothelial growth factor (VEGF) monoclonal antibody with a high binding specificity for VEGF.
The aim of this meta-analysis was to evaluate the effectiveness and safety of bevacizumab in patients with unresectable non-small-cell lung cancer (NSCLC) on the basis of evidence-based methodology.
The electronic database PubMed was searched to identify randomized, controlled trials (RCTs) of bevacizumab for the treatment of unresectable NSCLC. Other databases such as the Cochrane Library Trials Register, the WHO Trial Registration, the National Cancer Institute, ClinicalTrials.gov, the European Organization for Research and Treatment of Cancer, the Southwest Oncology Group, the Eastern Cooperative Oncology Group, the European Society of Clinical Oncology and the American Society of Clinical Oncology were also searched. The meta-analysis was performed using Reviewer Manager Version 5.0 software provided by the Cochrane Collaboration. Outcome measures were overall survival rates, progression-free survival, tumour response rate, incidence of severe adverse events (SAEs) and treatment-related death.
Four eligible studies that included 2101 patients were found; in these studies, bevacizumab was administered to 1237 patients. Neither high-dose (15 mg/kg) nor low-dose (7.5 mg/kg) bevacizumab increased 1-year overall survival rates compared with patients not treated with bevacizumab. However, high-dose bevacizumab, rather than low-dose, increased 2-year overall survival rate (risk ratio [RR] = 1.24; 95% confidence interval [CI] 1.04, 1.49) and tumour response rate (RR = 1.69; 95% CI 1.21, 2.35) compared with patients not treated with bevacizumab. Progression-free survival was also significantly improved in both the low- (hazard ratio [HR] = 0.76; 95% CI 0.64, 0.90) and high-dose groups (HR = 0.73; 95% CI 0.65, 0.81). There was a clear and significant increase in the rate of treatment-related death in the high-dose group (RR = 2.07; 95% CI 1.19, 3.59) compared with patients not treated with bevacizumab. No significant differences were noted in the rate of treatment-related death in the low-dose group or in the incidences of SAE in the low- or high-dose groups compared with patients not treated with bevacizumab. Neutropenia was easily induced in both the low- and high-dose bevacizumab groups. Patients who received high-dose bevacizumab tended to experience hypertension, neutropenia, haemoptysis, rash and headache more frequently than patients not treated with bevacizumab.
Low-dose bevacizumab may significantly improve progression-free survival in patients with unresectable NSCLC, whereas high-dose bevacizumab may increase 2-year overall survival rates, prolong progression-free survival and improve tumour response rate but at the cost of higher treatment-related death. Larger well designed RCTs should be carried out in the future to clarify the role of bevacizumab in the treatment of NSCLC.
Clinical Drug Investigation 01/2010; 30(4):229-41. · 1.82 Impact Factor
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ABSTRACT: Researches about blocking angiogenesis to treat tumor have become one of the most promising and active fields in anticancer research. This study aimed to investigate the eukaryotic expression of extracellular ligand binding domains of murine Tie-2 and its anti-angiogenesis effect.
A eukaryotic expression vector pcDNA3.1(+) integrating with a DNA fragment which encode extracellular ligand binding domains of murine Tie-2 was transfected into SGC-7901 gastric cancer cell line. The protein expression was detected by western blot analysis and immunocytochemistry staining. Following the construction of nude mouse tumor xenograft model with and without transfected cells, tumor microvessel density was determined by counting per high power field in the sections stained with an antibody to CD31 to test its inhibition of angiogenesis.
The extracellular ligand binding domains of murine Tie-2 receptor was highly expressed in SGC-7901 gastric cancer cells with plasmid transfection. The mean tumor sizes of groups with and without transfection were 1.27 +/- 0.35 and 1.75 +/- 0.17 cm(3), respectively (P = 0.025). The mean inhibitory rate of tumor was 27.18 +/- 19.93%. The comparison between highest microvessel density of group with transfection (14.00 +/- 3.80) and that of group without transfection (22.30 +/- 5.91) was statistically significant at P = 0.030.
The protein of extracellular ligand binding domains of murine Tie-2 can be expressed at high level in the eukaryotic expression system, and the expressed protein may have the anti-angiogenesis effect.
Journal of Gastroenterology and Hepatology 10/2009; 25(2):345-51. · 2.87 Impact Factor
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ABSTRACT: Fatty liver disease (FLD) is increasingly recognized as one of the most common chronic liver diseases in China. This study aimed to investigate the prevalence and risk factors of FLD in Chengdu, Southwest China, and to provide a relevant basis for the prevention and intervention of FLD.
Altogether 9094 subjects (4721 men and 4373 women) of over 18 years old who had received a medical checkup in the West China Hospital of Sichuan University between January and December 2007 were evaluated for FLD. FLD was diagnosed by ultrasonography. Body mass index (BMI), height, body weight, blood pressure, fasting plasma glucose (FPG), triglycerides (TG), total cholesterol (TCh), alanine aminotransferase (ALT), hepatitis B surface antigen (HBsAg), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were measured using routine laboratory methods.
The overall prevalence of FLD was 12.5%, which was more than 3-fold higher in males than in females (18.9% vs. 5.7%, X2=359.624, P<0.001). The prevalence increased with age in females and males of less than 50 years. The prevalence of alcoholic, suspected alcoholic, and non-alcoholic FLD was 2.6%, 3.6%, and 6.3%, respectively. Multiple logistic regression analyses showed that 10 factors (male sex, age, BMI, FPG, hypertension, TG, TCh, HDL-C, LDL-C, and ALT abnormalities) were closely related to FLD. In heavy drinkers, obesity increased the risk of FLD by 23.78-fold (95% CI, 10.22-55.33), but heavy drinking was only associated with a 2-fold (95% CI, 1.50-2.66) increased risk in obese subjects.
The prevalence of FLD among a health-checkup population in Chengdu, Southwest China was lower than the published for other areas of China. FLD in Chengdu adults was found to be closely associated with sex, age, BMI, and other metabolic syndrome features.
Hepatobiliary & pancreatic diseases international: HBPD INT 09/2009; 8(4):377-82. · 1.08 Impact Factor
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ABSTRACT: To investigate the expression of CTGF, CTGF mRNA, NF-kappaB and AP-1 induced by TGF-beta1 in human lung fibroblast (HLF-02), and study the effect and possible mechanism of rosiglitazone on signal pathways of TGF-beta1 in HLF-02.
The effects of TGF-beta1, curcumin, PDTC and rosiglitazone on the expression of CTGF, NF-kappaB and AP-1 were evaluated with Western blot. The expression of CTGF was also detected with immunohistochemistry assay. The level of CTGF mRNA was detected with RT-PCR.
(1) The CTGF protein levels of HLF-02 cells were significantly up-regulated after incubated with 1 ng/mL TGF-beta1 for 15 min (P<0.01 vs Control). The CTGF mRNA was also up-regulated. The levels of CTGF protein and CTGF mRNA expression was up-regulated by TGF-beta1 in a time-dependent manner. (2) The expression of NF-kappaB and AP-1 increased after HLF-02 cells were incubated with 1 ng/mL TGF-beta1 for 30 min (P<0.01 vs Control). The CTGF protein levels were inhibited obviously after HLF-02 cells were incubated with PDTC or curcumin. (3) The expression of CTGF, NF-kappaB and AP-1 decreased after pre-incubation with different doses of rosiglitazone (P<0.01 vs TGF-beta1 group). The CTGF mRNA were also markedly inhibited (P<0.01 vs TGF-beta1 group).
It is supposed that rosiglitazone inhibits CTGF expression induced by TGF-beta1 in HLF-02 cells by activating PPARgamma through NF-kappaB and AP-1 signal transduction pathways.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 07/2009; 40(4):598-603.
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ABSTRACT: To investigate the effects of small interference RNA (siRNA) targeting connective tissue growth factor (CTGF) on collagen synthesis induced by hypoxia in human lung fibroblasts.
CTGF-siRNA expression plasmid was stablely transfected into MRC-5. The transfected cells were cultured under hypoxia condition (37 degrees C, 5% CO2, 1% O2) for 1, 2, 4, 6 and 12 hours respectively. The expression of CTGF and collagen type I was assessed by real time quantitative PCR and Western blot.
Expression of CTGF mRNA was up-regulated as early as 1 hour in control scrambled-siRNA/MRC-5 cells after exposed to hypoxia condition, which remained the elevated level up to 6 hours. Compared with control cells, the expression of CTGF mRNA and protein induced by hypoxia was decreased significantly in CTGF-siRNA/MRC-5 cells. Moreover, the deposition of collagen type I in CTGF-siRNA/MRC-5 cells was also declined.
The siRNA targeting CTGF gene could efficiently inhibit collagen deposition induced by hypoxia in human lung fibroblasts.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 06/2009; 40(3):378-81.
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ABSTRACT: To investigate the risk factors for gallstone disease in the general population of Chengdu, China.
This study was conducted at the West China Hospital. Subjects who received a physical examination at this hospital between January and December 2007 were included. Body mass index, blood pressure, fasting plasma glucose, serum lipid and lipoproteins concentrations were analyzed. Gallstone disease was diagnosed by ultrasound or on the basis of a history of cholecystectomy because of gallstone disease. Unconditional logistic regression analysis was used to investigate the risk factors for gallstone disease, and the Chi-square test was used to analyze differences in the incidence of metabolic disorders between subjects with and without gallstone disease.
A total of 3573 people were included, 10.7% (384/3573) of whom had gallstone diseases. Multiple logistic regression analysis indicated that the incidence of gallstone disease in subjects aged 40-64 or >or= 65 years was significantly different from that in those aged 18-39 years (P < 0.05); the incidence was higher in women than in men (P < 0.05). In men, a high level of fasting plasma glucose was obvious in gallstone disease (P < 0.05), and in women, hypertriglyceridemia or obesity were significant in gallstone disease (P < 0.05).
We assume that age and sex are profoundly associated with the incidence of gallstone disease; the metabolic risk factors for gallstone disease were different between men and women.
World Journal of Gastroenterology 04/2009; 15(15):1886-91. · 2.47 Impact Factor
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ABSTRACT: To investigate whether corticosteroids are effective in the treatment of plastic bronchitis with hemoptysis.
A retrospective, clinical study was undertaken. Thirty two patients with only first episodes of plastic bronchitis with hemoptysis were divided into a steroid group (n=18) treated with glucocorticoids, and a non-steroid group (n=14). The supportive therapy was uniformly applied to both groups, except for glucocorticoids. Variables such as temperature and white blood cell counts were determined. Furthermore, the volume of hemoptysis and bronchial casts were evaluated in detail daily.
There was no difference in the demographic data and variables at baseline between both groups (all P>0.05). On days 5, 6, 7 and 8, the volume of hemoptysis was significantly decreased in the steroid group compared with the non-steroid group (43+/-15 mL vs 117+/-33 mL on d 5, 29+/-12 mL vs 97+/-23 mL on d 6, 18+/-10 mL vs 80+/-20 mL on d 7, and 13+/-8 mL vs 66+/-14 mL on d 8; all P<0.05), and on d 10 after fibreoptic bronchoscopy, the cases with bronchial casts was reduced evidently in the steroid group in comparison with the non-steroid group (OR=5.69, 95% CI=1.76-43.6; P=0.005). There was no significance in mechanical ventilation and mortality between both groups.
Despite some limitations of this study, it has been demonstrated that, on the basis of common supportive therapy, corticosteroids would be effective and safe for the treatment of plastic bronchitis with hemoptysis.
Acta Pharmacologica Sinica 09/2006; 27(9):1206-12. · 1.95 Impact Factor
