N Martín Casabona

University of Barcelona, Barcino, Catalonia, Spain

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Publications (14)65.17 Total impact

  • N Martín Casabona · J Rosselló Urgell
    Medicina Clínica 12/2000; 115(17):663-70. · 1.42 Impact Factor
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    ABSTRACT: 441 strain of mycobacteria were exposed to probes marked with luminous material belonged to M. tuberculosis complex and M. avium complex. We analyzed the following points: 1. If the cut-off points obtained with our strains were in accordance to those recommended by the manufacturer, using two different luminometers. 2. If the correlation constant makes possible the conversion of the units form one luminometer to the units obtained with the other one. 3. Data for sensibility, specificity and predictive positive and negative values using different cut-off points. Cut-off points were different in all cases except for the data obtained with one luminometer using a probe belonged to M. tuberculosis complex. In this case the correlation constant could not be used. In contrast, whichever the luminometer employed was, we found no significant differences between sensibility, specificity and predictive values.
    Revista Clínica Española 03/1993; 192(3):108-11. · 1.06 Impact Factor
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    ABSTRACT: We have studied 540 mycobacterial strains isolated in Lowestein-Jensen medium and 133 samples of different pathologic products against commercialized 125I-DNA Mycobacterium tuberculosis complex, Mycobacterium avitum-intracellulare and Mycobacterium gordonae. The sensitivity, specificity and positive and negative predictive values against isolated strains was 100% for the 3 studied probes. The 125I-DNA probe specific for M. tuberculosis complex is studied in samples with positive bacciloscopy; statistic values vary according to the cutting point of the different percentages of hybridization: at 1.5% the sensitivity, specificity and predictive negative and positive values are 38.6%, 71.4%, 97.5%, and 9.4% respectively, while if the cutting point percentage is 3% these values are: 33.1%, 100%, 100%, and 7.8% respectively. We believe that with these probes the identification time is limited to time needed for the incubation of prime cultures, and in some cases it can be performed on the day the samples reach the laboratory.
    Revista Clínica Española 10/1991; 189(4):167-71. · 1.06 Impact Factor
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    ABSTRACT: The yield of microscopy examination as a quick diagnostic test in several pulmonary and nonpulmonary samples referred to the mycobacterial laboratory of a general hospital is reviewed. During a 14-year period (1975-1988), 113,836 biological products were investigated. In 9,972 a positive culture for mycobacteria was obtained. For the microscopy examination the auramin technique was used; if positive, acid-alcohol resistance was confirmed by overstaining with the Ziehl-Neelsen technique. The culture was used as the reference method. Microscopic examination was positive in 34% of samples with a positive culture, being 39% for Mycobacterium tuberculosis and 10% for environmental mycobacteria. The overall specificity was 99%, the positive predictive value was 91% and the negative predictive value was 94%. In pleuropulmonary samples the sensitivity ranged from 48% in sputum and 2% in pleural biopsy, with specificity higher than 99%. In nonpulmonary samples, sensitivity, specificity and positive and negative predictive values varied with the type of sample. The false positive rate (positive microscopy with negative culture) was 0.3, and it was shown that 80% of these patients had received previous therapy. In organic fluids (pleural, peritoneal, cerebrospinal), the sensitivity was not greater than 13%. Sputum, bronchoaspirate and bronchoalveolar lavage were better for the diagnosis of tuberculosis than gastric aspirate. Approximately 1 in each positive microscopy examinations corresponded to environmental mycobacteria. In some nonpulmonary samples with high sensitivity the positive predictive value was low. 80% of the false positive results corresponded to previously treated patients.
    Medicina Clínica 08/1991; 97(6):211-4. · 1.42 Impact Factor
  • P Cruaud · J.T. Yamashita · N.Martin Casabona · F Papa · H.L. David
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    ABSTRACT: Serum IgG and IgM antibodies against a 2,3-diacyl-trehalose-2'-sulphate (SL-IV) antigen using ELISA were determined in controls (n = 288) and in leprosy (n = 210) and tuberculosis (n = 99) patients. In all assays, the amount of antigen per well was 100.0 ng and sera were diluted 1/250. In the case of leprosy, anti-SL-IV IgG and IgM antibody titres increased from the tuberculoid towards the lepromatous pole of the spectrum. In the tested population, the sensitivity of the assay was 93.2% in multibacillary leprosy and 33.3% in paucibacillary leprosy (specificity of 88.7%). Multibacillary patients with erythema nodosum leprosum (ENL) had lower titres than non-ENL. ELISA results were similar to those obtained using the Mycobacterium leprae phenolic glycolipid-I (PGL-I) antigen. In the case of tuberculosis (pulmonary and extrapulmonary), significant titres of anti SL-IV IgG and IgM antibodies were detected in about 75% of the patients using a cutoff point of 0.150, and in 51.6% using a cutoff of 0.300 (specificities were, respectively, 88% and 100%). We concluded that the determination of IgG and IgM antibodies against SL-IV was useful in leprosy and tuberculosis case finding program using a cutoff point of 0.150, and for serodiagnosis using a cutoff of 0.300.
    Research in Microbiology 07/1990; 141(6):679-94. DOI:10.1016/0923-2508(90)90062-U · 2.71 Impact Factor
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    ABSTRACT: The isolation of mycobacteria in abdominal specimens during a 10 years period is presented. Twenty-three clinical cases have been reviewed; patients were divided in three groups: 1) Peritoneal and intestinal tuberculosis. 2) Pulmonary tuberculosis with isolation of M. tuberculosis in feces, and 3) Miliary tuberculosis. We emphasize the low yielding of bacilloscopy, the low number of colonies in cultures and the importance of the microbiological study of abdominal specimens in the confirmatory diagnosis. The predominant symptoms of peritoneal tuberculosis were abdominal pain and distention and fever. The study of the ascitic fluid showed in most of the cases lymphocytic exudate and the pathological study of biopsies showed granulomas with caseous necrosis. Three patients had another associated abdominal disease. Isolation of M. tuberculosis in feces does not invariably mean the presence of intestinal tuberculosis. We confirm the frequent association of disseminated tuberculosis and HIV1 infection.
    Revista espanola de enfermedades digestivas: organo oficial de la Sociedad Espanola de Patologia Digestiva 07/1990; 77(6):409-13. · 1.41 Impact Factor
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    ABSTRACT: Sera from 38 tuberculous patients and 62 healthy controls (31 PPD skin test positive and 31 negative) were assayed, by enzyme-linked immunosorbent assay (ELISA), to test the activity of IgG and IgM antibodies against purified protein derivative (PPD) antigen and a phenolglycolipid antigen (PLG-Tb 1) isolated and purified from Mycobacterium tuberculosis strain Canetti. Using PPD antigen, the sensitivity and specificity were respectively, 50 and 93.5% for IgG and 71.1 and 59.7% for IgM antibody activity. Against PGL-Tb 1 antigen, IgG had a sensitivity of 94.7% and the specificity was 96.8%, for IgM antibody they were 65.8% and 75.8% respectively. The ELISA using PGL-Tb 1 antigen could be a useful way to develop a rapid technique to aid in the diagnosis of tuberculosis.
    Acta leprologica 02/1989; 7 Suppl 1:89-93.
  • J E Ollé Goig · N Martín Casabona · T González Fuente
    Medicina Clínica 03/1987; 88(7):261-3. · 1.42 Impact Factor
  • N Martín Casabona · T González Fuente · F Fernández Pérez
    Medicina Clínica 05/1985; 84(16):651-4. · 1.42 Impact Factor
  • T González Fuente · A M Calderón Ruiz · N Martín Casabona
    Medicina Clínica 12/1984; 83(15):648. · 1.42 Impact Factor
  • Medicina Clínica 12/1984; 83(16):690-1. · 1.42 Impact Factor
  • Medicina Clínica 07/1983; 81(3):138. · 1.42 Impact Factor
  • The Lancet 05/1977; 1(8018):956-7. DOI:10.1016/S0140-6736(77)92259-0 · 45.22 Impact Factor
  • Revista Espa de Cardiologia 39(3):227-9. · 3.79 Impact Factor