Shusheng Gong

Capital Medical University, Peping, Beijing, China

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Publications (47)6.58 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: We aimed to compare the characteristics between lentivirus and adenovirus vector mediated gene transfer into cultured spiral ganglion cells (SGCs). SGCs from newborn rats were cultured and exposed to lentivirus-GFP and adenovirus-GFP vectors. GFP expression and the cell morphology were evaluated under epi-fluorescence microscope at 3 days and 7 days after exposure. Survival number of SGCs was counted, and the average percentage of SGCs with GFP expression was calculated, and axon length was measured by ImageJ software. Cultured SGCs were transfected by either adenovirus or lentivirus vector successfully. The adenovirus vector presented an instant and efficient transfection. However, the expression of GFP went down after 7 days. In lentivirus-GFP group, GFP expression was detected at 7 days after exposure, and the number of cells with GFP expression increased gradually in the following days. Statistical analysis revealed that there were no differences in survival number of SGCs and average axon length among lentivirus-GFP group, adenovirus-GFP group and control group. Cultured SGCs can be transfected by either lentivirus vector or adenovirus vector safely and efficiently. SGCs are more susceptible to adenovirus vector, but GFP persists for a longer period after the lentivirus-mediated gene transfer.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 02/2011; 25(4):172-5.
  • Yong Fu, Song Pan, Qiang Liu, Shusheng Gong
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    ABSTRACT: To explore the survival rate of neural stem cells (NSCs) from fetal rat and its biological properties after cryopreservation and thawing. Different generations NSCs (the first generation, the third generation and the sixth generation) from fetal rat which cultivated with serum-free medium in vitro were cryopreserved in the cryogen, which were neurosphere culture medium with 10% BSA and 7.5% DMSO (without neural growth factor). The cryopreserved cells were resuscitated at 1st week, 4th week, 8th week, 12th week and 16th week respectively. The survival rate of cells were calculated and the cells were incubated and differentiated again. Different time of cryopreservation, different generations did not affect NSCs survival (P > 0.05) after cryopreservation. The survival rate of NSCs was from 60% to 70% after resuscitated, which were differentiated into neurons and astrocytes in 10% embryonic bovine serum (without growth factor). NSCs were successfully cryopreserved, resuscitated and recultured, which would create bases for the experimental study on the selected-date application of NSCs transplantation into cochlea for treating sensorineural deafness.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 04/2010; 24(7):311-4.
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    ABSTRACT: To explore the involvement of endoplasmic reticulum molecular chaperone GRP78 in the impairment of inner ear consistent with the mimetic aging model. Twenty-four Wistar rats were randomly divided into two groups. Model group was induced by daily hypodermic injection of 10% D-galactose (800 mg x kg(-1) x d(-1)) for 8 weeks and the control group was given saline accordingly. Spatial learning and memory was measured by Morris-Water-Maze. Colorimetry was used to analyze superoxide dismutase (SOD) and malondialdehyde (MDA) extracted from inner ear tissue. Hearing threshold of rats were detected with Auditory brainstem response (ABR). In addition, expression of GRP78 in the inner ear was detected by immunohistochemistry, RT-PCR and Western blot. The control group was studied parallel. The escape latency in the model group injected with D-galactose was markedly longer than that in the control group. Accordingly, the changes of SOD and MDA were more significant in the model group, the difference between two groups was significant (t-test, P<0.01). the variation of ABR in two groups was observed, There was no statistically difference of the hearing in the model group compared with the control group (P>0.05). The expression of GRP78 was significantly different between two groups, which is increased in the inner ear tissue of model group (P<0.01). The impairment of inner ear tissue partly dued to the oxidative stress in the model, which was induced by D-galactose and endoplasmic reticulum molecular chaperone was thought to contribute to the impairment mechanism of inner ear in mimetic aging model.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 01/2010; 24(1):28-32.
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    ABSTRACT: To investigate the mechanism of intense noise-induced cochlea cells death in guinea pig, and the effect of JNK signal transduction pathway in the procedure of cochlea cells apoptosis by intense noise-induced. Thirty-two guinea pigs were randomly divided into 4 groups. The guinea pigs in the experiment groups were exposed to 4 kHz narrow band noise at 120 dB SPL for 4 h. After the noise expose for 1, 4, 14 days of the experiment guinea pigs, ABR of the guinea pigs on experiment and control groups were tested before put them to death. Four guinea pig's cochleas of every group were taken to paraffin section, and the rest was extracted the total cochlear's protein. Apoptosis was tested by terminal deoxynucleotidyl Transferase (TdT)-mediated deoxyuridine triphosphate (d-UTP) nick and labeling method (TUNEL). The phosphorylation of JNK and c-Jun were tested by immunohistochemistry and western blot methods. Tunel-Positive cells in the Corti's, SGC and SV of experiment groups, and there have significant differences compared with the control group (P<0.01) and Tunel-Positive cells are most in 1 d experiment group. The positive cells of P-JNK and P-c-Jun could be detected in guinea pig's cochleas after noise exposed, but no positive cells were found in the control. Protein levels of P-JNK and P-c-Jun were risen up and activated quickly after noise exposed, and achieved peak in 1 d, 4 d and then fallen-offs, but still maintained higher levels within 14 d. Intense noise causes cochlea cell lesion by inducing apoptosis to result in and JNK signal transduction pathway plays an important role in the procedure of apoptosis.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 12/2009; 23(24):1138-42.
  • Linghui Luo, Shusheng Gong, Pei Chen
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    ABSTRACT: To make clear the molecular pathways involved in hydrogen peroxide-induced spiral ganglion cells death. The spiral ganglion cells of the newly born rats were primary cultured. Then the SGCs were exposed to hydrogen peroxide for different concentrations (0, 100, 200, 500 micromol/L) and for different hours (2, 4, 6 h). Cell nucleic were stained simultaneously with the DNA binding dyes Hoechst 33258 and propidium iodide. At lower concentrations of hydrogen peroxide, apoptosis was the main reason for cell death. At higher concentrations of hydrogen peroxide, the cells died mainly by necrosis. The effects of hydrogen peroxide are dose and time dependency. Reactive oxygen species may play a role as an early molecule signal in the mechanism of SGCs death.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 04/2009; 23(5):222-4.
  • Jianting Wang, Shusheng Gong, Yingpeng Liu
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    ABSTRACT: To study the effect on the expression of protein kinase CK2alpha and growth of human laryngeal carcinoma xenograft in nude mice by applying small interfering RNA (siRNA) specific to protein kinase CK2alpha. Human laryngeal carcinoma Hep-2 cells were implanted under the skin of nude mice. After the tumors grew to a definite size, the tumors were injected with siRNA expression plasmid specific to protein kinase CK2alpha. The weight and volume of subcutaneous tumors were measured. The expression level of protein kinase CK2alpha mRNA and protein of tumors were measured with reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical technique, respectively. Protein kinase CK2alpha mRNA and protein expressions were significantly decreased in tumors transfected with siRNA expression plasmid specific to protein kinase CK2alpha (P<0.05). The tumor grew slowly after transfected with siRNA expression plasmid specific to protein kinase CK2alpha (P<0.01). The siRNA expression plasmid specific to protein kinase CK2alpha may suppress the growth and the protein kinase CK2alpha expression of subcutaneous tumors. RNA interfering technology may be a new strategy for the treatment laryngeal cancer.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 04/2009; 23(6):267-70.
  • Xianhong Wang, Shusheng Gong
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    ABSTRACT: To study the expression of Eotaxin and Eotaxin-2 in nasal polyp and observe the effects of steroids on Eotaxin and Eotaxin-2 in nasal polyps. The SP immunohistochemical method was applied to explore the expression of Eotaxin and Eotaxin-2 in nasal polyps before and after systemic corticosteroids therapy; the optical density of positive cells were measured by using HPIAL-2000 image-conduct system. The expression of Eotaxin and Eotaxin 2 were positive in mucosal epithelia, vascular endothelial, glandular epithelium, and inflammatory cells. After corticosteroids use, the number of eosinophils, the expression of Eotaxin in mucosal epithelia, inflammatory cells and vascular endothelial, and the expression of Eotaxin-2 in mucosal epithelia were significantly decreased (P<0.05). The steroids affected the expression of on Eotaxin-2 in mucosal epithelia of nasal polyps mostly. 1) The expression of Eotaxin and Eotaxin-2 in nasal polyp are positive. 2) The effects of steroid on the nasal polyps may depend on decreasing the infiltration of eosinophils and the expression of Eotaxin and Eotaxin-2.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 04/2009; 23(5):205-8.
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    ABSTRACT: The aim of the study is to investigate the survival of neural stem cells (NSCs) in normal rat cochlea and their potential effect on auditory function and cochlea structures via round window transplantation. In comparison with the normal rats without any transplantation (group III), normal rat cochleae were transplanted with NSCs infected with adenovirus carrying green fluorescence protein (GFP) gene (group I) or the artificial perilymph (group II) via round windows. Auditory functions were monitored by thresholds of auditory brain stem responses (ABRs); the cochlea structures were examined by hematoxylin and eosin staining; survivals of implanted NSCs were determined by the expression of GFP; survivals of hair cells were accessed by whole mount preparation; and ultrastructures of hair cells were examined by scanning electron microscopy. There were significant differences in the click-ABR thresholds in rats among all 3 groups neither at pretransplantation nor at posttransplantation; there were no significant differences in these values before and after transplantation in the same rats from each group. After transplantation, the cochlea structures were normal in both group I and group II. Grafted NSCs were visualized by the GFP expression in every turn of the cochlea in all animals of group I. There were no significant differences in the losses of outer hair cells (OHCs) among 3 groups. The inner hair cells and most OHCs were normal in every turns of cochleae of all groups. Neural stem cells survived in normal rat cochlea after transplantation via round window and showed no obvious effects on auditory functions and inner ear pathologic examination of the rat cochlea.
    American journal of otolaryngology 01/2009; 30(1):8-16. · 0.77 Impact Factor
  • Pei Chen, Jun Song, Linghui Luo, Shusheng Gong
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    ABSTRACT: The remodeling process of synapses and neurotransmitter receptors of facial nucleus were observed. Models were set up by facial-facial anastomosis in rat. At post-surgery day (PSD) 0, 7, 21 and 60, synaptophysin (p38), NMDA receptor subunit 2A and AMPA receptor subunit 2 (GluR2) were observed by immunohistochemical method and semi-quantitative RT-PCR, respectively. Meanwhile, the synaptic structure of the facial motorneurons was observed under a transmission electron microscope (TEM). The intensity of p38 immunoreactivity was decreased, reaching the lowest value at PSD day 7, and then increased slightly at PSD 21. Ultrastructurally, the number of synapses in nucleus of the operational side decreased, which was consistent with the change in P38 immunoreactivity. NMDAR2A mRNA was down-regulated significantly in facial nucleus after the operation (P<0.05), whereas AMPAR2 mRNA levels remained unchanged (P>0.05). The synapses innervation and the expression of NMDAR2A and AMPAR2 mRNA in facial nucleus might be modified to suit for the new motor tasks following facial-facial anastomosis, and influenced facial nerve regeneration and recovery.
    Journal of Huazhong University of Science and Technology 12/2008; 28(6):714-8. · 0.58 Impact Factor
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    ABSTRACT: To investigate culturing neural stem cells (NSCs) from rat embryos in vitro and to observe their growth and differentiation. NSCs were isolated from hippocampus of SD rat embryos (P16-P18) and cultured in DMEM/F12 medium containing EGF, bFGF, B27. To observe process of cell proliferation by microscope and identify cell types by immunocytochemical analyses after differentiation. NSCs grew well in serum-free conditional medium and their cell bodies present transparent with good refraction at about eighth day. After differentiation, the cells demonstrated NSE and GFAP immunoreactive. NSCs were cultured well in serum-free conditional medium and they could be induced to differentiate into neurons and astrocytes in serum conditional medium.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 09/2008; 22(16):747-50.
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    ABSTRACT: To observe the changes of auditory electrophysiology and inner ear pathology in rat cochlea after the noise exposure, and to offer the experimental data for exploring the mechanism of noise-damaged cochlea. The rats in the study group were exposed to a intense narrow band noise centered at 4 kHz at the leave of 120 dB (SPL) for 4 h. The exposed cochleae were collected at various intervals (1 or 21 days) after the noise exposure. Auditory function was monitored by measuring thresholds of auditory brain stem responses (ABR). The morphological changes in rat cochlear hair cell (HC) were examined by HC nuclei stained with Propidium iodide (PI), a fluorescent dye specifically labelling the nuclear DNA and scanning electron microscopy (SEM). The number of spiral ganglion cells was calculated using pathologic technique. The thresholds of ABR in the study group were significantly greater than that in the normal control group (P < 0.01). Examined at 1 day after the noise exposure, normal, apoptosis, necrotic and missing out hair cell (OHC) could be distinguished with PI staining, whereas the apoptosis OHC were not found at 21 days. Significant OHC loss was found in as compared to the normal control group (P < 0.01). There was not significant difference in the calculation of spiral ganglion cells (P > 0.05). SEM revealed the injured stereocilia of OHC (disarrangement, collapse) and OHC loss in the study group, which was more severe in OHC3 than the other two rows of OHC. The intense noise used in our study could injure the rat cochlea and bring permanent threshold shift (PTS). Under this condition, the death modes of HC in the cochlea include apoptosis and necrosis in the fore part, whereas necrotic is the major mode in the evening of exposure. The injured stereocilia of OHC and OHC loss could remain the most consistent correlate of PTS.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 07/2008; 22(11):509-12.
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    ABSTRACT: To elucidate the relationship between the intratumoral lymphangiogenesis and lymphatic metastasis, and provide some theoretic evidence for the judgement of lymph node metastasis and prognosis of laryngeal squamous cell carcinoma (LSCC), also for the treatment. Immunohistochemical analysis was performed to 50 specimens of LSCC with lymphatic endothelial marker (Lymphatic vessel endothelial hyaluronan receptor-1 LYVE-1), the vascular endothelial marker CD34 and the pKi67 proliferation marker to record lymphatic vessel density (LVD). Quantitation of lymphangiogenesis growth factor VEGF-C by RT- PCR was performed to 30 specimens of LSCC. Finally the correlation between LVD and tumor TNM grade, VEGF-C mRNA, grade of diffraction was analyzed with statistics methods. Newly proliferating lymphatic vessel were observed in all LSCC. The median copy number of VEGF-c mRNA was 4-5-fold higher in LSCC than in adjacent normal tissue. There was correlation between tumor VEGF-C mRNA copy number and intratumoral LVD (n =30, P <0.05), there was no significant association between LVD and sex, T stage and grade of diffraction (n =50, P >0.05) but N stage (n = 50, P <0.05). Newly proliferating lymphatic vessels existed in LSCC. There was correlation between high levels of LVD in LSCC than in normal tissue. And the high level of VEGF-C may accelerate the lymphatic metastasis by promoting the proliferation of intratumoral lymphatic.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 05/2008; 22(9):385-8.
  • Pei Chen, Min Bao, Shanchun Yu, Shusheng Gong
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    ABSTRACT: To observe the recovery process of facial behavior and function in rat, and then to supply reliable functional parameters for the researches in such fields. Rat models of facial nerve paralysis were set up by sectioning and anastomosis of facial nerve. The behavioral change included whisker movement and blink reflex were observed weekly. Electroneurography (ENoG) and blink reflex (BR) were examined dynamically and all data were analyzed by statistic soft ware. Postoperatively, the whisker movement ceased, blink reflex was lost or sluggish but the fibrillation of vibrissae appeared. Whisker movement and evoke blink reflex were seen 1-2 months following operation gradually, which subsequently increased in intensity and frequency. Mass contraction of the periauricular muscles were observed at the same time as eye closure 2 month following operation. The latency of compound muscle action potential (CMAP) at experimental side began to prolong at 21 day, reached climax at 1 month and was stabilized at 3-4 month postoperatively, but it could not get full recovery. The latencies of 28-63 day were longer than other time points (P<0.05). The amplitude and intensity didn't change characteristically. The R1 can be observed repetitively, which disappeared at 7-14 day and gradually recovered 1 month following operation. At experimental side, the R1-type wave (R1oris) in orbicularis oris could be observed at the same time as R1 recorded 2 month following operation, which indicated the facial synkinesis, one hyperkinetic post-paralytic sequela happened. Then the latency of both R1 and R1oris decreased concomitantly. There were correlations between them, but only the significant difference of R1oris latency presented between 2 month and other time points (P<0.05). It is concluded that the methods of ENoG and BR could examine the recovery process of facial movement, which would help studying the pathophysiological mechanism of facial nerve injury and regeneration after being revised.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 04/2008; 22(7):318-21.
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    ABSTRACT: To purify P0 protein from guinea pig's inner ear by preparative SDS-PAGE and study the possible role it may play in the etiology of autoimmune inner ear disease. A mixture of membraneous proteins of inner ear was separated by preparative SDS-PAGE. The corresponding band at 30kd was cut and electrically eluted. The protein collected was identified by analytical SDS-PAGE and Western blot assay. A group of 20 guinea pigs were immunized with P0 protein emulsified in complete Freund's adjuvant, another 10 guinea pigs were immunized with complete Freund 's adjuvant only as control. The guinea pigs' hearing thresholds, serum IgG level and morphological changes in the inner ear were investigated. The distribution of P0 protein in the cochlear was detected by immunohistochemical technique. The purity of the protein was demonstrated by a single band at the 30 kD site in SDS-PAGE, which was identified as P0 protein by western blot analysis assay. About 17.5% P0-immunized guinea pigs showed increased hearing thresholds, elevated IgG level (F =6.48, P <0. 01), as well as a decreased number of spiral ganglion cells and inflammatory cell infiltration in the cochlear nerve region. The P0 protein is distributed in the cochlear nerve and spiral ganglion only. P0 protein from guinea pig's inner ear can be successfully purified by preparative SDS-PAGE and an animal model of experimental autoimmune inner ear disease induced by P0 protein is successfully established.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 08/2007; 21(16):748-51.
  • E Zhang, Shusheng Gong
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    ABSTRACT: To investigate the expression of Hypoxia inducible factor-1alpha (HIF-1alpha) and inducible Nitric Oxide Synthase (iNOS) in cholesteatoma and approach the possible role of them in the formation and development of the middle ear cholesteatoma. Immunohistochemical SP method was used to examine the expression of HIF-1alpha and iNOS protein in 21 middle ear cholesteatoma specimens and 11 samples of normal external ear canal skin. In the 21 middle ear cholesteatoma, in epithelium tissue samples and 11 external ear channel's normal skin specimens, the expression index of HIF-1alpha was 52.49 +/- 13.80, 0.60 +/- 0.49, and that of iNOS was 92.05 +/- 27.84, 1.15 +/- 0.84 respectively. The HIF-1alpha and iNOS expression index of cholesteatoma epithelium was significantly higher than that of external ear channel's normal skin (P < 0.01). Moreover in the cholesteatoma epithelium, linear correlation analysis showed that iNOS protein was positively correlated with HIF-1alpha protein expression (r = 0.536, P < 0.05). High density of HIF-1alpha and iNOS are found in the epithelium of cholesteatoma; Hypoxia and its relative factors HIF-1alpha,iNOS may play an important role in the formation and development of cholesteatoma.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 06/2007; 21(10):463-5.
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    ABSTRACT: To improve the possibility of diagnosis of the Mondini dysplasia. The clinical manifestation and the examination of CT and MRI and surgical treatment of the Mondini dysplasia were discussed. Because Mondini dysplasia with cerebrospinal fluid leak would occur recurrent meningitis, the patients were often difficult to be diagnosed. Especially. if the defect was unilateral, it was frequently unrecognized. The patients with recurrently unclear cerebrospinal fluid leak and meningitis would be suspected. The diagnosis of the disease is based on the examination of the temporal bone CT and MRI. To the patients with cerebrospinal fluid leak, a transtympanic closure is one of very effective management.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 05/2007; 21(7):312-4.
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    ABSTRACT: To explore the methods of culture, identification and label of embryonic rat neural stem cells. The cells isolated from fetal rat hippocampus were identified with nestin immunocytochemical fluorescent staining. The cellular multiplication was observed by immunocytochemical fluorescence co-label after accession of BrDU. The neural stem cells (NSCs) were marked with fluorescent dye, bisbenzimide (Hoechest33342) and induced to differentiate. The differentiated cells were detected with Neuron Specific Enolase (NSE) and Glial Fibrillary Acidic Protein (GFAP) immunocytochemical fluorescent staining respectively. Nest-like clusters of neural stem cells were obtained in suspension and the cells could be differentiated into neurons and astrocytes which maintaining the main characteristics of NSCs after 8 passages of culture. The label efficiency of cells with Hoechest33342 was 97% and no attenuation of fluorescent brightness was observed after 8 passages of culture. The cellular fluorescence was observed in the NSCs and the differentiated cells. The cells from embryonic rat hippocampus possessed the abilities of division, multiplication and self-renew, which were believed to be the main characteristics of NSCs of the central nervous system. The cells could be efficiently labeled with fluorescent dye and could be used as donor cells in experimental research on NSCs transplantation.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 03/2007; 21(4):172-5.
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    ABSTRACT: In order to study the effect of 5, 6-Dichloro-1-beta-D-ribofuranosyl-benzimidazole (DRB) on the biological characteristics of human laryngeal carcinoma Hep-2 cell line in vitro, Hep-2 cells cultured in vitro were treated with different concentrations of DRB. Changes in cell proliferation, apoptotic rate and invasiveness were detected by MTT assay, flow cytometry (FCM) and matrigel in vitro invasion assay, respectively. It was found that DRB inhibited the proliferation of Hep-2 cells in a dose-and time-dependent manner. After being treated with 0, 10, 20, 40, 80 microm mol/L DRB for 24 h, the apoptotic rate in Hep-2 cells was (0.68+/-0.19)%, (1.95+/-0.12)%, (8.51+/-0.26)%, (11.26+/-0.17)% and (14.99+/-0.32)%, respectively. The matrigel in vitro invasion assay revealed that DRB began to inhibit the invasion of Hep-2 cells at the concentration of 5 microm mol/L, and with the increase of DRB concentration, the inhibitory effect was enhanced. It was suggested that DRB could influence the essential biological characteristics of Hep-2 cells, inhibit Hep-2 cells proliferation, reduce invasive ability and induce apoptosis of Hep-2 cells.
    Journal of Huazhong University of Science and Technology 03/2007; 27(1):104-6. · 0.58 Impact Factor
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    ABSTRACT: To study the suppression of proliferation of Hep-2 cells by stable expression of anti-sense perlecan cDNA. In this study, the plasmids of recombination eukaryotic expression vector perlecan anti-sense cDNA (pAP) were transfected into Hep-2 cells by using cationic liposome (lipofectamine 2000) and divided into three groups: non-transfected group, WT group; transfection with no load carrier, neo group; and transfection with the pAP plasmid, pAP group. Semi quantify RT-PCR, western blot assay and MTT assay were used to detected the expression of perlecan mRNA and protein in the three groups; the level of cell proliferation; and the responsivity of basic fibroblast growth factor (bFGF). It was showed that the expression of perlecan mRNA and protein were significantly reduced in the pAP group compared with WT group and ph beta Apr-neol transfected group ( P < 0.01). In the presence of 1 microg/L of bFGF in low serum (0.1% FCS), the pAP transfected cells showed a reduced proliferation rate (MTT assay) while the wild type cells and ph beta Apr-neol transfected cells grew rapidly. The growth of Hep-2 cells could be inhibited significantly by perlecan anti-sense cDNA plasmids transfection.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 11/2006; 20(21):984-7.
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    ABSTRACT: To describe the clinical presentation of ossifying fibroma of the temporal bone, and to discuss its diagnosis and treatment. A rare case of ossifying fibroma of the temporal bone was presented. The patient was a 8-year-old boy with a one year history of left ear discomfort, suppurative otorrhea and progressive hearing loss. Physical examination of the ear revealed a stenosis of left external auditory canal (EAC), and an obvious expansion at mastoid region. Audiometry showed no hearing of the left ear. A postauricular approach was used to expose the mass. Frozen section evaluation during operation was not definitive but suggested benign nature in histology. The tumor was fully resected. The EAC was sealed by sutured skin, and the extended mastoid cavity was obliterated with abdominal fat. The final pathological report indicated the stromal cells were negative for S-100 protein and epithelia membrane antigen (EMA), supporting the diagnosis of ossifying fibroma. One-year follow-up showed the sealed EAC was satisfactory with complete interior and no tumor recurred. Ossifying fibroma of the temporal bone is a rare entity, which is a benign neoplasm but may show an aggressive behavior by compression and encroachment upon adjacent structures. Early and complete removal was advocated.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 11/2006; 20(19):868-70.

Publication Stats

38 Citations
6.58 Total Impact Points


  • 2009–2011
    • Capital Medical University
      • Department of Otorhinolaryngology Head and Neck Surgery
      Peping, Beijing, China
  • 2009–2010
    • Zhejiang University
      • School of Medicine
      Hangzhou, Zhejiang Sheng, China
  • 2004–2009
    • Huazhong University of Science and Technology
      • Department of Otorhinolaryngology, Head and Neck Surgery
      Wu-han-shih, Hubei, China
  • 2006
    • Wuhan Union Hospital
      Wu-han-shih, Hubei, China
  • 2005
    • Wuhan University of Science and Technology
      Wu-han-shih, Hubei, China