Hasan Basri Ila

Publications (10)10.25 Total impact

• Article: The genotoxic and antigenotoxic effects of tannic acid in human lymphocytes.

  Mehmet Buyukleyla, Sebile Azirak, Eyyup Rencuzogullari, Ayse Yavuz Kocaman, Hasan Basri Ila, Mehmet Topaktas, Cengiz Darici
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  ABSTRACT: The genotoxicity of tannic acid (TA, tannin) were investigated using chromosome aberration (CA), sister chromatid exchange (SCE), and micronucleus (MN) test systems in human peripheral lymphocytes. Also, the antigenotoxicity of TA against known mutagen EMS was also examined. The lymphocytes were treated with 1.74 × 10(-5), 3.49 × 10(-5), and 6.98 × 10(-5) µM of TA for 24- and 48-hour treatment periods. For the antigenotoxicity of TA, the lymphocytes were treated with three different concentrations of TA and 2.71 µM of EMS. TA synergically induced the CA alone and with the mixture of EMS. However, TA did not induce the SCE alone, whereas TA and EMS as a mixture also synergically induced SCE. TA alone showed no clear effect on micronucleus formation, and it did not induce the MN when used with EMS as a mixture. In addition, TA showed a synergistic cytotoxic effect by decreasing the mitotic and nuclear division indices. The replication index was decreased at all concentrations for 48 hours of treatment time by TA and EMS as a mixture.
  Drug and Chemical Toxicology 07/2011; 35(1):11-9. · 1.08 Impact Factor
• Source

  Available from: Erman Salih Istifli

  Article: The genotoxic and antigenotoxic effects of Aloe vera leaf extract in vivo and in vitro

  Ahmet KAYRALDIZ, Ayşe YAVUZ KOCAMAN, Eyyüp RENCÜZOĞULLARI, Erman Salih İSTİFLİ, Hasan Basri İLA, Mehmet TOPAKTAŞ, Yusuf Kenan DAĞLIOĞLU
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  ABSTRACT: The genotoxic and antigenotoxic effects of Aloe vera leaf extract (AV) were investigated using the chromosome aberrations (CAs) test for the bone marrow cells of rats, sister chromatid exchanges (SCEs) and micronucleus (MN) and CAs tests for human lymphocytes, and the Ames Salmonella/microsome test system. In the bone marrow cells of rats, AV extract significantly induced structural and total CAs at all concentrations and in all treatment periods. In human peripheral lymphocytes, AV did not increase the mean SCE; however, it significantly induced the MN frequency and structural CAs. In addition, AV showed a cytotoxic effect by decreasing the replication index (RI), mitotic index (MI), and nuclear division index (NDI) in human lymphocytes and by decreasing the MI in the bone marrow cells of rats. AV did not decrease the genotoxicity or cytotoxicity of urethane (ethyl carbamate, EC) in the bone marrow cells of rats or in the mitomycin-C (MMC) in human lymphocytes. AV was a weak mutagen in the TA98 strain of Salmonella typhimurium in the absence of S9mix; however, AV+NPD (4-nitro-o-phenylenediamine) and AV+SA (sodium azide) exhibited a synergism in increasing the number of revertants for the TA98 and TA100 strains in the absence of S9mix, respectively.
  Turkish Journal of Biology. 01/2010;
• Article: Genotoxic potential of cyfluthrin.

  Hasan Basri Ila, Mehmet Topaktas, Eyyup Rencuzogullari, Ahmet Kayraldiz, Lale Donbak, Y Kenan Daglioglu
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  ABSTRACT: Cyfluthrin (CAS no. 68359-37-5), a synthetic fluorinated pyrethroid insecticide, is widely used in the home environment and in agriculture because of its high activity against a broad spectrum of insect pests and its low animal toxicity. There are no adequate data on genotoxic effects of cyfluthrin. The aim of this study was to analyze the potential genotoxic effects of cyfluthrin. The genotoxicity of cyfluthrin was evaluated, in vitro, by assessing the ability of the insecticide to induce gene mutation (evaluated using the Ames/microsome test), chromosomal aberrations (CA), sister chromatid exchange (SCE) and micronucleus (MN) formation in cultured human peripheral blood lymphocytes. Additionally, CAs and cytotoxicity induced by cyfluthrin were investigated in rat (Rattus norvegicus var. Albinos) bone-marrow cells to assess in vivo genotoxicity of cyfluthrin. The counts of reverse mutations in Salmonella typhimurium were not significantly increased (P>0.05). The frequency of CAs in human lymphocytes, treated with any concentration of cyfluthrin (500, 1000 or 2000 microg/ml) for a 24-h period, was not significantly increased (P>0.05). In contrast, CA was significantly increased for the highest two concentrations (1000 and 2000 microg/ml) in the 48-h treatment group compared with the control group (dimethyl sulfoxide, DMSO). Micronucleus formation was significantly (P<0.05) increased for all doses after the 48-h treatment, although the frequency of SCE did not increase significantly (P>0.05). Mitotic index (MI), proliferation index (PI) and nuclear division index (NDI) decreased significantly (P<0.05) due to the potential cytotoxicity of cyfluthrin, especially after the 48-h treatment period. The frequency of chromosome aberrations in bone-marrow cells of rats treated with the test substance increased significantly (P<0.05) for all doses (250, 500 and 1000 mg/kg body weight) for the two treatment periods (12 and 24 h) and the two administration routes, viz. intraperitoneal injection (i.p.) and oral gavage (gvg). In vivo cytotoxicity of cyfluthrin was detected only after administration by gavage for the 24-h treatment period. All these findings were not dose-dependent.
  Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 07/2008; 656(1-2):49-54. · 2.85 Impact Factor
• Article: The genotoxic effect of potassium metabisulfite using chromosome aberration, sister chromatid exchange, micronucleus tests in human lymphocytes and chromosome aberration test in bone marrow cells of rats.

  Ayse Yavuz-Kocaman, Eyyup Rencuzogullari, Hasan Basri Ila, Mehmet Topaktas
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  ABSTRACT: Potassium metabisulfite (PMB) is used as an antimicrobial substance in many kinds of foods. In the present study, the effects of PMB on chromosome aberrations (CAs), sister chromatid exchanges (SCEs), and micronucleus (MN) formation in human lymphocytes and as well as its effect on CAs in bone marrow cells of rats were investigated. The human lymphocytes were treated with 25, 50, 100, and 200 microg/ml of PMB for 24 and 48 hr. PMB was also intraperitoneally (ip) injected to the rats as a single dose of 150, 300, and 600 mg/kg body weight (b.w.) for 12 and 24 hr before sacrifice. PMB induced abnormalities such as structural and numerical (total) CAs, SCEs, and MN formations in a dose dependent manner in the lymphocytes of the 24- and 48-hr treatment periods. In addition, PMB showed a cytotoxic effect by decreasing the replication index (RI), mitotic index (MI) and nuclear division index (NDI) in a dose dependent manner in human lymphocytes. The compound induced CA as well and decreased the MI in bone marrow cells of rats. It might be concluded that PMB had a high genotoxic and cytotoxic risk.
  Environmental and Molecular Mutagenesis 06/2008; 49(4):276-82. · 3.71 Impact Factor
• Article: The effects of food protector biphenyl on sister chromatid exchange, chromosome aberrations, and micronucleus in human lymphocytes.

  Eyyüp Rencüzoğullari, Sebnem Parlak, Hasan Basri Ila
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  ABSTRACT: The aim of this study was to determine the possible genotoxic effects of biphenyl (E230), which is used as an antimicrobial agent in food by using sister chromatid exchanges (SCEs), chromosome aberrations (CAs), and micronucleus (MN) tests in human peripheral lymphocytes. The human peripheral lymphocytes were treated with four concentrations of biphenyl (10, 30, 50, and 70 microg/mL) for 24- and 48-h treatment periods. In the present study, biphenyl significantly increased the frequency of SCEs, CAs, and the frequency of MN when compared with both untreated control and solvent (dimethyl sulfoxide) control. The inductions of these abnormalities were in a dose-dependent manner. Biphenyl was capable to induce the structural CAs instead of numerical CAs. Biphenyl also showed a cytotoxic effect by decreasing the replication index at the highest two concentrations for 48 h and nuclear division index at the highest two concentrations for the 24- and 48-h treatment periods. However, biphenyl did not affect the mitotic index (MI).
  Drug and Chemical Toxicology 02/2008; 31(2):263-74. · 1.08 Impact Factor
• Article: The genotoxic effect of the new acaricide etoxazole.

  Eyyüp Rencüzoğullari, Hasan Basri Ila, Ahmet Kayraldiz, Mehmet Arslan, Songül Budak Diler, Mehmet Topaktaş
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  ABSTRACT: Etoxazole is a member of the diphenyl oxazoline class of insecticide was newly developed for use on pome fruits, cotton and strawberries as a acaricide. In the present study, genotoxic effects of acaricide etoxazole (ETX) (miticide/ovicide) were investigated using chromosome aberration (CA) test, sister chromatid exchange (SCE) test and micronucleus test in human lymphocytes. ETX induced the CAs at all concentrations (5, 10 and 20 microg/ml) for 24 h and also induced the CA at the highest concentration (20 microg/ml) for 48 h only. The inducing the CAs for 48 hours treatment period was dose-dependent. Besides, it induced the SCE at all concentrations and treatment periods in a dose-dependent manner as well. Although, ETX decreased the mitotic index (MI) at all concentration and treatment periods dose-dependently, while it did not decrease the replication index (RI) when compared to the negative and solvent controls. In addition, ETX induced the micronucleus at all concentrations except 5 microg/ml for 48 h. This inducing was in a dose-dependent manner as well. In conclusion, it can be concluded that ETX has a potential genotoxic effects in cultured human peripheral lymphocytes.
  Genetika 12/2004; 40(11):1571-5. · 0.44 Impact Factor
• Source

  Available from: Songül Budak Diler

  Article: Genotoxicity of aspartame.

  Eyyüp Rencüzoğullari, Berrin Ayaz Tüylü, Mehmet Topaktaş, Hasan Basri Ila, Ahmet Kayraldiz, Mehmet Arslan, Songül Budak Diler
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  ABSTRACT: In the present study, the genotoxic effects of the low-calorie sweetener aspartame (ASP), which is a dipeptide derivative, was investigated using chromosome aberration (CA) test, sister chromatid exchange (SCE) test, micronucleus test in human lymphocytes and also Ames/Salmonella/ microsome test. ASP induced CAs at all concentrations (500, 1000 and 2000 microg/ml) and treatment periods (24 and 48 h) dose-dependently, while it did not induce SCEs. On the other hand, ASP decreased the replication index (RI) only at the highest concentration for 48 h treatment period. However, ASP decreased the mitotic index (MI) at all concentrations and treatment periods dose-dependently. In addition, ASP induced micronuclei at the highest concentrations only. This induction was also dose-dependent for 48 hours treatment period. ASP was not mutagenic for Salmonella typhimurium TA98 and TA100 strains in the absence and presence of S9 mix.
  Drug and Chemical Toxicology 09/2004; 27(3):257-68. · 1.08 Impact Factor
• Article: No significant increase in chromosome aberrations and sister chromatid exchanges in cultured human lymphocytes treated with spiramycin.

  Eyyüp Rencüzoğullari, Hasan Basri Ila, Mehmet Topaktaş, Ahmet Kayraldiz, Songül Budak, Mehmet Arslan
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  ABSTRACT: In this study, the chromosomal aberrations (CAs) and sister chromatid exchanges (SCEs) were investigated in human lymphocytes treated with spiramycin antibiotic (trade name, rovamycin). Spiramycin did not induce the CAs and SCEs, and also did not decrease the mitotic index (MI). However, spiramycin decreased the replication index (RI) only at 48 h treatment times.
  Teratogenesis Carcinogenesis and Mutagenesis 02/2002; 22(1):51-8.
• Article: Chromosome aberration and sister chromatid exchange in workers of the iron and steel factory of Iskenderun, Turkey.

  Mehmet Topaktaş, Eyyüp Rencüzoğullari, Hasan Basri Ila, Ahmet Kayraldiz
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  ABSTRACT: The aim of this study was to investigate, by using chromosome aberration (CA) and sister chromatid exchange (SCE) tests, whether or not the workers employed in the Iskenderun (Turkey) iron and steel factory have any genotoxic risk. The CA and the SCE were investigated in 48 males employed in a coke ovens unit and 8 males employed in a product side unit of the factory and in control groups. The frequency of CA was higher while the frequency of the SCE was not in all the smoker-nonsmoker workers than in smoker-nonsmoker control groups. In addition, there was no significant decrease in the RI, while the MI was significantly lower than in the controls. .
  Teratogenesis Carcinogenesis and Mutagenesis 02/2002; 22(6):411-23.
• Article: Chromosome aberrations and sister chromatid exchanges in cultured human lymphocytes treated with sodium metabisulfite, a food preservative

  Eyyüp Rencüzoǧullari, Hasan Basri İla, Ahmet Kayraldiz, Mehmet Topaktaş
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  ABSTRACT: The aim of this study was to investigate the ability of sodium metabisulfite (SMB) which is used as an antimicrobial substance in food, to induce chromosome aberrations (CA) and sister chromatid exchanges (SCE) in human lymphocytes. SMB-induced CAs and SCEs at all concentrations (75, 150 and 300 μg/ml) and treatment periods (24 and 48 h) dose-dependently. However, SMB decreased the replication index (RI) and the mitotic index (MI) at the concentrations of 150 and 300 μg/ml for 24 and 48 h treatment periods. This decrease was dose-dependent as well.
  Mutation Research/Genetic Toxicology and Environmental Mutagenesis.