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ABSTRACT: Ninety-seven acetic acid bacteria, which were isolated from fruits, flowers and other material collected in Thailand by an
enrichment culture approach, were assigned to the genusAcetobacter by phenotypic and chemotaxonomic characterisations. On the basis of 16S–23S rRNA gene ITS restriction and 16S rRNA gene sequence
analyses, the ninety-seven isolates were grouped into three groups and identified at the specific level: 1) group A including
fifty-three isolates, which were identified asAcetobacter pasteurianus, 2) group B including forty-two isolates, which were identified asAcetobacter orientalis and 3) group C including two isolates, which were identified asAcetobacter lovaniensis. There was no isolate to be assigned to other 15 species of the genusAcetobacter.
Annals of Microbiology 04/2012; 58(2):319-324. · 0.69 Impact Factor
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ABSTRACT: Two hundred and eighty-eight arsenic-resistant bacteria were isolated by an enrichment culture method from a total of 69 arsenic-contaminated
soil-samples collected from Dantchaeng district in Suphanburi province (47 samples), and from Ron Phiboon district in Nakhon
Sri Thammarat province (22 samples), in Central and Southern Thailand, respectively. Twenty-four of the 288 isolated arsenic-resistant
bacteria were found to be arsenite-oxidizing bacteria. On the basis of their morphological, cultural, physiological, biochemical
and chemotaxonomic characteristics, and supported by phylogenetic analysis based upon their 16S rRNA gene sequences, they
were divided into five groups, within the genera Acinetobacter, Flavobacterium, Pseudomonas, Sinorhizobium and Sphingomonas, respectively. Within genera, phylogenetic analysis using the 16S rRNA gene sequences suggested that they were comprised
of at least ten species, five isolates being closely related to known bacteria (Acinetobacter calcoaceticus NCCB 22016T, Pseudomonas plecoglossicida FPC951T, Ps.knackmussii B13T, Sinorhizobium morelense Lc04T, and Sphingomonas subterranea IFO16086T). The other five proposed species are likely to be new species closely related to Flavobacterium johnsoniae, Sinorhizobium morelense, Acinetobacter calcoaceticus and Pseudomonas plecoglossicida, but this awaits further characterization for confirmation of the taxonomic status. No overlap in isolated species or strains
was observed between the two sites. The strain distribution and characterization are described.
World Journal of Microbiology and Biotechnology 04/2012; 24(12):3091-3096. · 1.53 Impact Factor
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ABSTRACT: An actinomycete strain, SH2-13T, was isolated from a marine sediment sample collected from the Andaman Sea of Thailand. Applying a polyphasic approach, the isolate was identified as a member of the genus Micromonospora using morphological and chemotaxonomic characteristics, including the presence of meso-diaminopimelic acid in the peptidoglycan, whole cell sugars were arabinose, galactose, glucose, rhamnose, ribose and xylose. Diagnostic polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphoglycolipids. The major menaquinones were MK-10(H2), MK-10(H4) and MK-10(H6). The 16S rRNA gene sequence analysis revealed the similarity to Micromonospora marina JSM1-1T (99.1 %), Micromonospora coxensis 2-30-b/28T (99.1 %), Micromonospora aurantiaca DSM 43813T (98.8 %) and Micromonospora chalcea DSM 43026T (98.7 %). However, a combination of DNA-DNA hybridization results and phenotypic properties indicated that strain SH2-13T (=NBRC 107934T =BCC 45601T) should be classified as the type strain of a novel species, Micromonospora sediminicola sp. nov. is proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2012; · 2.11 Impact Factor
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ABSTRACT: Strain D10-9-5T was isolated from Mangrove soil, Samutsakhon province in Thailand. A polyphasic taxonomy were studied to determine the taxonomic position of the strain. The strain presented single rough spore on substrate mycelium, no aerial mycelium. Chemotaxonomic data supported strain D10-9-5 was assigned to the genus Micromonospora by showing meso-diaminopimelic acid and glycolyl muramic acid in the peptidoglycan, ribose, mannose, galactose, xylose and glucose as whole cell sugars, MK-10(H4)(14.8%), MK-10(H6) (46.7%), and MK-10(H8)(27.5%) as predominant isoprenoid quinone, iso-C15:0 (19.5%), anteiso-C17 :0 (15.4%), iso-C17 :1ω9c (11.0%), and C17 : 1ω8c (10.7%) as major cellular fatty acid, phosphatidylglycerol and phosphatidylethanolamine as phospholipid in the cell wall. The 16S rDNA gene sequence and phylogenetic tree analysis showed strain D10-9-5 was closely related with Micromonospora marina JCM 12870T (99.6%), Micromonospora coxensis JCM 13248 T (99.4%), Micromonospora aurantiaca JCM 10878T (99.3%), Micromonospora humi JCM15292T (99.3%), and Micromonospora chalcea JCM 3031T (99.1%). Strain D10-9-5T could be clearly distinguished from related Micromonospora species by its physiological and biochemical characteristics as well as its phylogenetic position and level of DNA-DNA relatedness. Therefore, the strain represents a novel species for which the name Micromonospora maritima sp. nov. is proposed; the type strain is D10-9-5T (=JCM 17013 T =NBRC 108767T =PCU 322T=TISTR 2000T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2012; · 2.11 Impact Factor
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ABSTRACT: Forty-five acetic acid bacteria, which were isolated from fruits, flowers and other materials collected in Thailand by an
enrichment culture approach, were assigned to the genusGluconobacter by phenotypic and chemotaxonomic characterisations. On the basis of 16S–23S rRNA gene ITS restriction and 16S rRNA gene sequence
analyses, the forty-five isolates were grouped into five groups and identified at the specific level as follows: 1) seventeen
isolates were grouped into Group A and identified asG. oxydans; 2) twelve isolates were grouped into Group B and identified asG. cerinus; 3) nine isolates were grouped into Group C and identified asG. frateurii; 4) six isolates were included into Group D and identified asG. thailandicus; 5) one isolate was grouped into Group E, characterised by a restriction pattern comprised of 667 and 48-bp fragments inAvaII digestion, differing from those of strains of Group C orG. frateurii and Group D orG. thailandicus, and unidentified.
Annals of Microbiology 04/2012; 58(4):741-747. · 0.69 Impact Factor
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ABSTRACT: Highly arsenic resistant bacteria (27 isolates), which had a minimum inhibitory concentrations (MICs) for arsenite and arsenate
of ⩾ 40 mM and > 400 mM, respectively, were isolated from tannery wastes and agricultural soils collected in Central Thailand.
On the basis of the morphological, cultural, physiological and biochemical characteristics, and on the principal ubiquinone
component and 16S rRNA gene sequence analyses, they were identified as nine isolates each ofKlebsiella (Groups 1 and 8) andAcinetobacter (Groups 2, 3 and 7), four isolates each ofPseudomonas (Groups 4 and 6) andComamonas (Group 5), and one isolate ofEnterobacter (Group 9). From these 27 isolates, only one isolate, A3-3 from the genusComamonas, appeared potentially capable of oxidizing arsenite to arsenate, as determined by silver nitrate staining of arsenite agar
plates after colony growth.
Key wordsarsenic resistant bacteria-tannery waste-agricultural soil-arsenite oxidation
Annals of Microbiology 04/2012; 59(4):649-656. · 0.69 Impact Factor
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The Journal of General and Applied Microbiology 01/2012; 58(5):397-404. · 0.98 Impact Factor
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The Journal of General and Applied Microbiology 01/2012; 58(3):245-51. · 0.98 Impact Factor
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ABSTRACT: Halobacterium piscisalsi was proposed by Yachai et al. (2008), with a single strain, HPC1-2(T) ( = BCC 24372(T) = JCM 14661(T) = PCU 302(T)), which was isolated from fermented fish (pla-ra) in Thailand. According to Yachai et al. (2008), the strain was closely related to Halobacterium salinarum based on 16S rRNA gene sequence comparisons and could be differentiated by low DNA-DNA relatedness values and different biochemical profiles compared with other species of the genus. The reanalysis of the 16S rRNA gene sequences and the DNA-DNA relatedness among H. piscisalsi JCM 14661(T) and H. salinarum strains JCM 8978(T), R1 and NRC-1 revealed that they all had exactly the same 16S rRNA gene sequence and shared more than 70 % DNA-DNA relatedness. In addition, the full-length DNA-dependent RNA polymerase subunit B (RpoB) protein sequence of H. piscisalsi JCM 14661(T) (607 amino acids) was the same as that of H. salinarum JCM 8978(T) and showed 94.7 and 96.7 % similarities with those of Halobacterium noricense JCM 15102(T) and Halobacterium jilantaiense JCM 13558(T), respectively. Despite the different biochemical properties described by Yachai et al. (2008), the characteristic phenotypic properties of H. piscisalsi agreed with those in the description of H. salinarum emended by Gruber et al. (2004). Therefore, H. piscisalsi Yachai et al. (2008) should be regarded as a later heterotypic synonym of H. salinarum Elazari-Volcani 1957.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2011; 62(Pt 9):2160-2. · 2.11 Impact Factor
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ABSTRACT: A cellulose-degrading bacterium, strain FCN3-3(T), was isolated from buffalo faeces collected in Nakhonnayok province, Thailand. The strain was characterized based on its phenotypic and genotypic characteristics. Strain FCN3-3(T) was a Gram-positive, aerobic, spore-forming, rod-shaped bacterium. It contained meso-diaminopimelic acid in cell-wall peptidoglycan. The major menaquinone was MK-7. Anteiso-C(15:0) (52.5%), iso-C(16:0) (18.9%) and C(16:0) (9.1%) were the predominant cellular fatty acids, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol were the major phospholipids. The DNA G+C content was 58.0 mol%. Phylogenetic analysis using 16S rRNA gene sequences showed that strain FCN3-3(T) was affiliated to the genus Cohnella and was closely related to Cohnella phaseoli GSPC1(T), Cohnella luojiensis HY-22R(T) and Cohnella hongkongensis HKU3(T), with 97.2, 96.8 and 96.3% sequence similarity, respectively. Strain FCN3-3(T) could be clearly distinguished from all known species of the genus Cohnella by its physiological and biochemical characteristics as well as its phylogenetic position and level of DNA-DNA relatedness. Therefore, the strain represents a novel species of the genus Cohnella, for which the name Cohnella cellulosilytica sp. nov. is proposed; the type strain is FCN3-3(T) ( = KCTC 13645(T) = TISTR 1996(T) = PCU 323(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2011; 62(Pt 8):1921-5. · 2.11 Impact Factor
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ABSTRACT: Three strains, RBY-1(T), PHD-1 and PHD-2, were isolated from fruits in Thailand. The strains were Gram-negative, aerobic rods with polar flagella, produced acetic acid from ethanol and did not oxidize acetate or lactate. In phylogenetic trees based on 16S rRNA gene sequences and 16S-23S rRNA gene internal transcribed spacer (ITS) sequences, the strains formed a cluster separate from the type strains of recognized species of the genus Gluconobacter. The calculated 16S rRNA gene sequence and 16S-23S rRNA gene ITS sequence similarities were respectively 97.7-99.7 % and 77.3-98.1 %. DNA G+C contents ranged from 57.2 to 57.6 mol%. The strains showed high DNA-DNA relatedness of 100 % to one another, but low DNA-DNA relatedness of 11-34 % to the tested type strains of recognized Gluconobacter species. Q-10 was the major quinone. On the basis of the genotypic and phenotypic data obtained, the three strains clearly represent a novel species, for which the name Gluconobacter nephelii sp. nov. is proposed. The type strain is RBY-1(T) ( = BCC 36733(T) = NBRC 106061(T) = PCU 318(T)), whose DNA G+C content is 57.2 mol%.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 09/2011; 61(Pt 9):2117-22. · 2.11 Impact Factor
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ABSTRACT: Two strains of Gram-stain-positive, catalase-negative, tetrad-forming cocci, C01(T) and C02, were isolated in Thailand from fish sauce. They were facultatively anaerobic, non-motile and non-spore-forming bacteria. These strains produced l-lactic acid from glucose. They grew at pH 5.0-9.0, at 15-40 °C and in the presence of 10 % (w/v) NaCl. The dominant fatty acid was C(18 : 1)ω9c. The DNA G+C contents of strains C01(T) and C02 were 38.6 and 38.7 mol%, respectively. Strain C01(T) was related most closely to Desemzia incerta DSM 20581(T), with a 16S rRNA gene sequence similarity of 96.9 %. The strains could be distinguished clearly from D. incerta DSM 20581(T) based on cell morphology, physiological and biochemical characteristics and low levels of DNA-DNA relatedness. On the basis of the data presented, strains C01(T) and C02 are considered to represent a novel species of a new genus in the Bacillus-Lactobacillus cluster, for which the name Pisciglobus halotolerans gen. nov., sp. nov. is proposed. The type strain of Pisciglobus halotolerans is C01(T) ( = KCTC 13150(T) = TISTR 1958(T) = PCU 316(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 07/2011; 61(Pt 7):1688-92. · 2.11 Impact Factor
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ABSTRACT: Fermented rice flour (khao-khab, a non-glutinous rice) and related products are Thai traditional products. The types of acetic acid bacteria (AAB) microflora in khao-khab have not been reported. In this study, Acetobacter strains were isolated and identified based on the phenotypic and chemotaxonomic characteristics and molecular aspects.
Twenty-five acetic acid bacteria isolated from fermented rice products and a starter for sweetened rice in Thailand by an enrichment culture approach, were assigned to the genus Acetobacter by phenotypic and chemotaxonomic characterisations. On the basis of the 16S rRNA gene sequence and 16S-23S rRNA gene ITS restriction analyses, 25 isolates were divided into six groups and identified at the specific level: (1) Group 1 included five isolates, which were identified as A. indonesiensis; (2) Group 2 included two isolates, which were identified as A. lovaniensis; (3) Group 3 included one isolate, which was identified as A. orientalis; (4) Group 4 included eleven isolates, which were identified as A. pasteurianus; (5) Group 5 included three isolates, which were identified as A. syzygii and (6) Group 6 included three isolates, which were unidentified and considered to constitute a new species.
Results revealed that various Acetobacter species were distributed in Thai fermented rice flour and related products. A novel Acetobacter species was isolated from the product.
Journal of the Science of Food and Agriculture 06/2011; 91(14):2652-9. · 1.44 Impact Factor
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ABSTRACT: A novel actinomycete, strain P0402(T), was isolated from peat swamp forest soil collected in Thailand. Its taxonomic position was determined by using a polyphasic taxonomic approach. The chemotaxonomic characteristics of this strain matched those of the genus Micromonospora, i.e. the presence of meso-diaminopimelic acid and N-glycolyl muramic acid in the peptidoglycan, whole-cell sugar pattern D, phospholipid type II, and cellular fatty acid type 3b. Phylogenetic analysis based on 16S rRNA gene sequences revealed a close relationship between strain P0402(T) and Micromonospora coxensis JCM 13248(T) (99.0 % similarity), Micromonospora eburnea JCM 12345(T) (99.0 %), Micromonospora marina JCM 12870(T) (98.9 %), Micromonospora halophytica JCM 3125(T) (98.7 %), Micromonospora chalcea JCM 3031(T) (98.7 %), Micromonospora purpureochromogenes JCM 3156(T) (98.6 %) and Micromonospora aurantiaca JCM 10878(T) (98.5 %). It could be clearly distinguished from these type strains based on low levels of DNA-DNA relatedness and phenotypic differences. On the basis of the data presented, strain P0402(T) is suggested to represent a novel species of the genus Micromonospora, for which the name Micromonospora humi sp. nov. is proposed. The type strain is P0402(T) ( = JCM 15292(T) = PCU 315(T) = TISTR 1883(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2011; 61(Pt 5):1176-81. · 2.11 Impact Factor
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ABSTRACT: Two actinomycete strains, CM9-9(T) and AK2-48, which produced straight rod-shaped, non-motile cells, were isolated from soils in Chiang Mai and Phuket provinces, respectively, Thailand. The morphological and chemotaxonomic characteristics of the isolates coincided with those of the genus Agromyces. Phylogenetic analysis using 16S rRNA gene sequences also indicated that the isolates were clearly separated from their closest relative, Agromyces aurantiacus YIM 21741(T), and should be classified in the genus Agromyces. Furthermore, a combination of DNA-DNA hybridization results and physiological and biochemical properties indicated that the isolates could be distinguished from all recognized members of the genus Agromyces. The isolates therefore represent a novel species, for which the name Agromyces tropicus sp. nov. is proposed. The type strain is CM9-9(T) (=JCM 15672(T) =BCC 34764(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2011; 61(Pt 3):605-9. · 2.11 Impact Factor
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ABSTRACT: Isolates AH11(T) and AH13(T) were isolated from flowers of lantana and candle bush respectively collected in Thailand. In phylogenetic trees based on 16S rRNA gene sequences, the two isolates formed an independent cluster, which was then connected to the type strain of Saccharibacter floricola. The calculated pair-wise 16S rRNA gene sequence similarities of isolate AH11(T) were 95.7-92.3% to the type strains of the type species of the 12 genera of acetic acid bacteria. The DNA base composition was from 51.2 to 56.8 mol % G+C, with a range of 5.6 mol %. When isolate AH11(T) was labeled, DNA-DNA similarities were 100, 12, 4, 5, and 4% respectively to isolates AH11(T) and AH13(T) and the type strains of Saccharibacter floricola, Gluconobacter oxydans, and Acetobacter aceti. The two isolates were non-motile and did not oxidize either acetate or lactate. No growth was found in the presence of 0.35% acetic acid w/v. The two isolates were not osmophilic but osmotolerant, produced 2,5-diketo-D-gluconate from D-glucose, and did not oxidize lactate, thus differing from strains of Saccharibacter floricola, which showed weak lactate oxidation. The two isolates contained unsaturated C(18:1)ω7c fatty acid as the major fatty acid, and were unique in the presence of a considerable amount of straight-chain C(18:1)2OH fatty acid. Q-10 was present as the major isoprenoid quinone. Neokomagataea gen. nov. was proposed with the two species, Neokomagataea thailandica sp. nov. for isolate AH11(T) (=BCC 25710(T)=NBRC 106555(T)), which has 56.8 mol % G+C, and Neokomagataea tanensis sp. nov. for isolate AH13(T) (=BCC 25711(T)=NBRC 106556(T)), which has 51.2 mol % G+C.
Bioscience Biotechnology and Biochemistry 03/2011; 75(3):419-26. · 1.28 Impact Factor
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The Journal of General and Applied Microbiology 01/2011; 57(3):159-67. · 0.98 Impact Factor
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The Journal of General and Applied Microbiology 01/2011; 57(5):293-301. · 0.98 Impact Factor
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ABSTRACT: Two xylan-degrading bacteria, strains MX15-2(T) and MX21-2(T), were isolated from soils collected in Nan province, Thailand. Cells were Gram-reaction-positive, facultatively anaerobic, spore-forming and rod-shaped. They contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major menaquinone was MK-7. iso-C(16 : 0) and anteiso-C(15 : 0) were the predominant cellular fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol were the major polar lipids. The genomic DNA G+C contents of strains MX15-2(T) and MX21-2(T) were 63.0 and 65.1 mol%, respectively. Phylogenetic analysis using 16S rRNA gene sequences showed that strains MX15-2(T) and MX21-2(T) were affiliated with the genus Cohnella and were closely related to Cohnella thermotolerans CCUG 47242(T), with 96.5 and 95.6 % sequence similarity, respectively. The strains could be clearly distinguished from each other and from all known species of the genus Cohnella based on their physiological and biochemical characteristics as well as their phylogenetic positions and levels of DNA-DNA hybridization. Therefore, these two strains represent novel species of the genus Cohnella, for which the names Cohnella xylanilytica sp. nov. (type strain MX15-2(T) =KCTC 22294(T) =PCU 309(T) =TISTR 1891(T)) and Cohnella terrae sp. nov. (type strain MX21-2(T) =KCTC 22295(T) =PCU 310(T) =TISTR 1892(T)) are proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2010; 60(Pt 12):2913-7. · 2.11 Impact Factor
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ABSTRACT: Forty-one isolates of moderately halophilic bacteria were isolated from fermented fish (pla-ra) in Thailand. On the basis of their phenotypic and chemotaxonomic characteristics, DNA-DNA relatedness and 16S rRNA gene sequences analyses, they were divided into six groups. The isolates in Group I to V were Gram-positive rod-shaped bacteria. They contained meso-diaminopimelic acid in the cell-wall peptidoglycan and menaquinone with seven isoprene units (MK-7). An isolate in Group VI was a Gram-negative rod-shaped bacterium. The DNA G+C contents of tested strains ranged from 36.5-63 mol%. Ten strains (Group I) were identified as Virgibacillus dokdonensis, 13 isolates (Group II) as V. halodenitrificans, 14 isolates (Group III) as V. marismortui, 1 isolate (Group IV) as Virgibacillus sp., 2 isolates (Group V) as Bacillus vietnamnensis, and 1 isolate (Group VI) as Chromohalobacter salexigens. Isolate MS3-4 in Group IV was closely related to V. carmonensis KCTC 3819(T) (95.9%). This strain contained anteiso-C(15:0) (55.8%) and anteiso-C(17:0) (17.7%) as major cellular fatty acids and had phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid as polar lipids. The DNA G+C content of MS3-4 was 38.0 mol%. The strain from Group IV is proposed as Virgibacillus siamensis sp. nov. and MS3-4(T) is the type strain (JCM 15395(T) =PCU 312(T) =TISTR 1957(T)).
The Journal of General and Applied Microbiology 10/2010; 56(5):369-79. · 0.98 Impact Factor
