J Valle

Universidad de Extremadura, Cáceres, Extremadura, Spain

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Publications (19)35.51 Total impact

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    ABSTRACT: A study was made of elastase production in 273 staphylococcal strains isolated from healthy goats by two different methods. In a soluble elastin medium, 20.2% of the strains tested showed elastolytic activity but no strains showed such activity in an insoluble elastic medium.
    Letters in Applied Microbiology 06/2008; 11(5):264 - 266. · 1.63 Impact Factor
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    ABSTRACT: A microbiological study was made of 100 strains of Fusobacterium necrophorum isolated from hepatic abscesses in bovine and ovine herds. Differences between the biological activity and ribotypes within the two F. necrophorum subspecies were studied. Conventional methods identified 89 isolates as F. necrophorum subsp. necrophorum and 11 as F. necrophorum subsp. funduliforme. For ribotyping, 50 strains (35 F.n. subsp. necrophorum, 11 F.n. subsp. funduliforme and 4 reference strains) were digested with restriction endonucleases (HindIII, EcoRI and BamHI) and examined after hybridization with digoxigenin-labelled cDNA probe transcribed from a 16 and 23S rRNAs from Escherichia coli. The most discriminating restriction endonuclease enzymes for ribotyping were EcoRI and BamHI. The presence or absence of two distinct band of 5 kb (EcoRI) and 10.5 kb (BamHI) differentiated the two subspecies. This technique also revealed genetic differences between isolates which could be used in the epidemiological study of clinical processes caused by F. necrophorum.
    Anaerobe 11/1998; 4(5):213-8. · 2.02 Impact Factor
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    ABSTRACT: Four hundred and sixty strains of obligate anaerobes were isolated from 216 cases of ovine foot rot distributed throughout Spain. The predominant species was Dichelobacter nodosus, which was isolated in 168 cases (77.8 per cent). A higher proportion of the strains of D nodosus than of the other strains had elastolytic activity, 118 of the 168 strains degrading elastin. Species belonging to the genus Prevotella were isolated from 96 cases (44.4 per cent). Serotyping of the D nodosus strains showed that serovars A1, A2 and C were the most commonly isolated in Spain.
    The Veterinary record 02/1998; 142(3):60-3. · 1.80 Impact Factor
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    ABSTRACT: Minimum inhibitory concentrations for 35 antimicrobial agents against 100 Fusobacterium necrophorum isolates from hepatic abscesses in sheep and cattle were determined. Twelve of the thirteen beta-lactam antibiotics tested inhibited growth of 100% of strains tested. Of the remaining antimicrobial agents, extensive susceptibility was found for: spiramycin, josamycin, lincomycin, tylosin, oxytetracycline, chlortetracycline, rufloxacin, metronidazole, cotrimoxazole, sulfadimethoxine, virginiamycin and fosfomycin.
    Journal of Veterinary Pharmacology and Therapeutics 03/1997; 20(1):21-3. · 1.35 Impact Factor
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    ABSTRACT: The in vitro activities of 14 anti-microbial agents were determined against 92 strains of E. coli isolated from lambs (60 strains) and kids (32 strains) affected by neonatal diarrhoea. The overall percentage of resistant strains to streptomycin, sulphadimethoxine and tetracycline was very high (above 70%). A high level of resistance (from 30% to 50%) to ampicillin, kanamycin, neomycin and chloramphenicol was also detected. The E. coli strains were highly susceptible to cephalosporins, polymyxin and quinolones. Most of the strains showed multiresistance: 77.2% of isolates were resistant to at least two antibiotics, 55.4% were resistant to at least four antibiotics and 33.7% were resistant to at least six antibiotics. A total of 34 antibiotypes could be distinguished.
    Journal of Veterinary Pharmacology and Therapeutics 11/1996; 19(5):397-401. · 1.35 Impact Factor
  • The Veterinary record 08/1996; 139(1):17-9. · 1.80 Impact Factor
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    ABSTRACT: In this study the susceptibility of 91 methicillin-resistant and -susceptible Staphylococcus intermedius strains (MRSI and MSSI, respectively) against 15 antimicrobial agents was determined. The activity of the antimicrobial agents was studied at pH 7.2 and pH 8.5. Methicillin was more active at pH 7.2 (28 strains methicillin-resistant) than at pH 8.5 (55 strains methicillin-resistant). Gentamicin showed excellent activity, with only 3 strains resistant at pH 8.5. However, gentamicin would have to be administered parenterally. Oxytetracycline cannot be recommended for treatment of canine staphylococcal dermatitis, due to the high percentage (over 25%) of strains that were found to be resistant. Clindamycin showed little activity in inhibiting growth of the strains studied, the percent resistance at pH 7.2 was 93.4%. Rifampin behaved differently at the two pH values. However, a close relationship was noted between methicillin-resistant and rifampin-resistant strains, particularly at the lower pH. Of the fluoroquinolones, ciprofloxacin or enrofloxacin would be a good useful alternative for the treatment of methicillin-resistant strains of S. intermedius. Lastly, very high resistance to sulphamethoxypyridazine was found, as was the case with trimethoprim and a combination of trimethoprim/sulphamethoxypyridazine, against not only MRSI but also MSSI strains.
    Journal of Veterinary Pharmacology and Therapeutics 05/1996; 19(2):118-23. · 1.35 Impact Factor
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    ABSTRACT: A study was made of the susceptibility of 91 strains of Staphylococcus intermedius, isolated from staphylococcal dermatitis of dogs, to 11 beta-lactam antimicrobial agents. The activity of these antimicrobial agents against all strains was tested at pH 7.2 and 8.5. Finally, a comparative study was made of the susceptibility of these micro-organisms, dividing them into six groups: beta-lactamase positive and negative, methicillin resistant and susceptible and oxacillin resistant and susceptible. Only three of the 11 antimicrobial agents studied exhibited satisfactory behaviour at the two pH levels studied: mezlocillin, cefazolin and cefalotine. There was a good correlation at pH 8.5 between methicillin-resistant strains and those resistant to penicillin G, ampicillin, amoxycillin, cefamandole and cefuroxime. This correlation was not observed, however, between oxacillin-resistant strains and strains resistant to the other antimicrobial agents studied.
    Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B 08/1995; 42(5):293-300.
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    ABSTRACT: A study was made of the sensitivity of 39 clinical isolates of anaerobic bacteria to 10 antimicrobial agents. Minimal inhibitory concentrations (MICs) were calculated using a new method--the E-test (AB Biodisk, Solna, Sweden)--and compared with those obtained using the conventional agar dilution method. Agreement between the MICs obtained by the two methods with a variation of +/- 2 dilutions was 78.7%. The E-test, though less sensitive than the conventional agar dilution method, may be of value in clinical veterinary practice when rapid selection of treatment for a given infectious process is required.
    Letters in Applied Microbiology 07/1995; 20(6):345-8. · 1.63 Impact Factor
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    ABSTRACT: A study was made of the susceptibility of 91 strains of Staphylococcus intermedius, isolated from staphylococcal dermatitis of dogs, to 11 ß-lactam antimicrobial agents. The activity of these antimicrobial agents against all strains was tested at pH 7.2 and 8.5. Finally, a comparative study was made of the susceptibility of these micro-organisms, dividing them into six groups: ß-lactamase positive and negative, methicillin resistant and susceptible and oxacillin resistant and susceptible. Only three of the 11 antimicrobial agents studied exhibited satisfactory behaviour at the two pH levels studied: mezlocillin, cefazolin and cefalotine. There was a good correlation at pH 8.5 between methicillin-resistant strains and those resistant to penicillin G, ampicillin, amoxycillin, cefamandole and cefuroxime. This correlation was not observed, however, between oxacillin-resistant strains and strains resistant to the other antimicrobial agents studied.
    Journal of Veterinary Medicine Series B 01/1995; 42:293-300. · 1.48 Impact Factor
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    ABSTRACT: The in vitro activities of four fluoroquinolone compounds were tested against 92 Escherichia coli strains of ovine and caprine origin under aerobic and anaerobic incubation conditions. The four fluoroquinolones proved to be highly effective against the E. coli isolates tested. When bacteria were cultured anaerobically, at least fourfold increases in the MICs of enoxacin for the strains occurred and no detectable changes in enrofloxacin, sparfloxacin, and ciprofloxacin MICs occurred.
    Antimicrobial Agents and Chemotherapy 11/1994; 38(10):2469-70. · 4.57 Impact Factor
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    ABSTRACT: A microbiological study was made of an outbreak of foot-rot in lambs. Elastolytic activity was detected in all 10 Bacteroides nodosus strains isolated from the hooves of 9 affected animals. Among 14 other isolated strictly anaerobic microorganisms belonging to different species or genera only 4 strains showed a low degree of elastolysis. The 14 antimicrobial agents studied effectively inhibited "in vitro" growth of B. nodosus.
    Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B 06/1992; 39(3):181-6.
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    ABSTRACT: The agar dilution method was used to determine the inhibitory activities of 28 antimicrobial agents against 35 strains of the genus Peptostreptococcus, 4 strains of the species Peptococcus niger, 20 strains of the species Megasphaera elsdenii, 7 strains from the species Acidaminococcus fermentans, 8 strains of the genus Clostridium, 11 strains of the genus Eubacterium, and 1 strain of the species Propionibacterium acidipropionici, all of which were isolated from 125 clinical cases of ovine foot rot between January 1987 and December 1988. The three unreidopenicillins studied proved to be the most active antimicrobial agents, with a high percentage of strains being susceptible at a concentration of 64 micrograms/ml. Penicillin G, ampicillin, and the three cephalosporins studied also had good activity. Fosfomycin showed a high degree of activity among the 116 anaerobic bacteria tested.
    Antimicrobial Agents and Chemotherapy 02/1992; 36(1):198-201. · 4.57 Impact Factor
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    ABSTRACT: The elastolytic activities of 82 Bacteroides nodosus strains were studied. Two substrates, insoluble elastin and soluble elastin, were used for this purpose. Roughly 15% of the strains which did not digest insoluble elastin were elastolytic with soluble elastin, the latter providing greater sensitivity, speed, and objectivity than its insoluble counterpart.
    Journal of Clinical Microbiology 10/1991; 29(9):2079-81. · 4.07 Impact Factor
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    ABSTRACT: The ability of staphylococcal strains isolated from different anatomical sites in 133 healthy goats to produce toxic shock syndrome toxin 1 (TSST-1) and the presence of antibodies to this toxin in serum and milk were studied. The enzyme-linked immunosorbent assay method was used to detect both the toxin and the presence of antibodies. Of a total of 342 staphylococcal strains studied, 86 (25.2%) were found to produce TSST-1. Specific antibodies to TSST-1 were found in the serum of 57 (42.9%) of the animals studied and the milk of 63 (47.4%) of the animals. These results suggest that goats are frequently in contact with staphylococci that produce TSST-1, a toxin usually associated with Staphylococcus aureus strains isolated from cases of toxic shock syndrome in humans.
    Applied and Environmental Microbiology 04/1991; 57(3):889-91. · 3.95 Impact Factor
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    ABSTRACT: A study was made of the staphylococcal population on the skin and on the nasal mucosa and in the milk of 133 healthy goats. Of a total of 346 strains isolated and characterised as belonging to the genus Staphylococcus, 74 (21.4%) were coagulase-positive (68 S. aureus and 6 S. hyicus), and 272 (78.6%) coagulase-negative. The novobiocin-sensitive species S. haemolyticus (23.5%), S. warneri (16.5%), S. epidermidis (11.8%), S. chromogenes (8.5%), S. caprae (6.6%) and S. hyicus (2.6%), and the novobiocin-resistant species S. xylosus (8.5%), S. sciuri (7.4%), S. saprophyticus (4.8%), S. cohnii (2.2%), S. lentus (1.1%), S. equorum (1.1%) and S. kloosii (1.1%) were identified. Twelve (4.4%) of coagulase-negative strains remained unidentified. Strains isolated in the skin of the udder and teats of the 133 goats were mainly novobiocin-sensitive coagulase-negative staphylococci, the most prevalent species being S. haemolyticus, S. warneri and S. epidermidis. Staphylococci indicative of subclinical infection were determined in the milk of 47 (35.3%) of the 133 goats sampled.
    Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B 04/1991; 38(2):81-9.
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    ABSTRACT: A study was made of the presence of antibodies (Ab) to staphylococcal enterotoxins A to E (SEA-SEE) in the serum and milk of 133 healthy goats, using a competitive ELISA method. Antibodies to some enterotoxins were detected in 83 sera (62.4%) and in 41 (30.8%) milk samples. In serum, antibodies to all SE types were detected, the most frequent being antibodies to SEA (24.8%). Milk contained antibodies to SEA, SEB and SEC, the latter being the most frequent (24.8%). A statistical study was performed to correlate the number of animals harbouring antibodies to a given enterotoxin with the presence in these animals of staphylococci producing that enterotoxin.
    FEMS microbiology immunology 03/1991; 3(1):53-8.
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    ABSTRACT: The ability of 342 staphylococcal isolates from different anatomical sites in healthy goats to produce staphylococcal enterotoxins (SE) was investigated. SE were produced by 74.3% of the 70 coagulase-positive strains and by 22% of the coagulase-negative strains studied. Most enterotoxigenic strains were isolated from the skin of udders and teats and from milk. SEC was the SE type most frequently produced, either alone (67.9%) or in combination with others. Five coagulase-negative species not previously reported as SE producers were identified (Staphylococcus chromogenes, S. warneri, S. sciuri, S. saprophyticus, and S. lentus). SEA, SEB, and SEC were detected in the milk of 17 of the 133 healthy goats studied. These results suggest that the goat is an important reservoir of enterotoxigenic staphylococci, most of which produce SEC.
    Applied and Environmental Microbiology 06/1990; 56(5):1323-6. · 3.95 Impact Factor