E I Riabchikova

Publications (16)0 Total impact

• Article: [Comparative analysis of the susceptibility and productivity of respiratory tract target cells of mice and rats exposed to inflienza virus in vitro].

  V A Zhukov, L N Shishkina, A A Sergeev, E M Malkova, E I Riabchikova, V A Petrishchenko, A N Sergeev, N V Ustiuzhanina, Iu V Nesvizhskiĭ, A A Vorob'ev
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  ABSTRACT: The levels of susceptibility to influenza virus A/Aichi/2/68 H3N2 and the virus yield were determined using primary cells of the trachea and lungs of CD-1 mice and Wistar rats, and for 3 sets of cells obtained from primary lung cells of the both species by centrifugation in the gradient of density and by sedimentation on a surface. The values of ID50 virus dose for 10(6) cells and virus yield per 1 infected cell determined for primary mice cells were 4.0+/-0.47 and 3.2+/-0.27 IgEID50 (lung cells), 3.8+/-0.17 and 3.3+/-0.20 IgEID50 (tracheal cells), and those determined for primary rat cells were 4.0+/-0.35 and 2.1+/-0.24 IgEID50 (lung cells), 3.7+/-0.27 and 2.2+/-0.46 IgEID50 (tracheal cells). The values of ID50 and yield measured for mixtures of cells obtained from primary lung cells by centrifugation in gradient of density and by sedimentation on a surface differed insignificantly (p = 0.05) from the values of the corresponding parameters measured for lung and tracheal cells for both rats and mice. The analysis of data on the variation of the concentrations of different cell types in the experimental cell mixtures shows that type 1 and 2 alveolocytes possess significantly lower (p = 0.05) susceptibility and productivity vs. ciliated cells of the both species. The investigation was conducted within the frame of the ISTC/DARPA#450p project.
  Vestnik Rossiĭskoĭ akademii meditsinskikh nauk / Rossiĭskaia akademiia meditsinskikh nauk 02/2008;
• Article: [A new setup for the generation and studies of mono-disperse microbiological aerosols in medical-and-biological research].

  V A Zhukov, A S Safatov, O V P'iankov, V S Toporkov, A A Sergeev, S A Kiselev, V A Iashin, N M Beliaev, E I Riabchikova, A V Zhukov, L N Shishkina, A A Medvedev, V A Petrishchenko, A N Sergeev, A A Vorob'ev
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  ABSTRACT: A setup for the generation and studies of mono-disperse microbiological aerosols is described in the paper. Coefficients of 3 microm aerosol deposition in the respiratory tract of mice and rats were refined by using the above setup. The probability of deposition of such particles in the trachea and lungs of mice was proven to be equal to 1.2 +/- 0.1% and 2.6 +/- 0.2%, respectively. The probability for rats was equal to 3.2 +/- 0.2 and 11.8 +/- 0.9%, respectively. The distribution of deposited aerosol particles was determined by electron microscopy.
  Vestnik Rossiĭskoĭ akademii meditsinskikh nauk / Rossiĭskaia akademiia meditsinskikh nauk 02/2004;
• Article: [Pulmonary cell susceptibility in mice and rats to influenza virus when infected in vivo and in vitro].

  A A Sergeev, L N Shishkina, V A Zhukov, A N Sergeev, V A Petrishchenko, I V Fankin, O V P'iankov, E I Riabchikova, E M Malkova, A A Vorob'ev
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  ABSTRACT: The purpose of the case study was to evaluate comparatively the relative contribution of cell susceptibility and the inhibiting effect of factors of pulmonary epithelial lining in mice and rats to influenza virus A/Aichi/2/68 (H3N2) adapted to mice as related with the development of infection process in the lungs of experimental animals when infected in vivo and in vitro. Mice and rats were infected aerogenically with different doses of influenza virus. The primary cell-culture suspensions sampled from the lungs of mice and rats were used to study the adsorption and dynamics of influenza virus production in infection by different dose of influenza virus in vitro. The cell suspensions were shown to be able to produce the influenza virus for as long as 48 hours after infection. It was for the first time that the results denoted the identical susceptibility of primary pulmonary cells in mice and rats to influenza virus. A lower pulmonary susceptibility to influenza virus in rats versus mice could be indicative of that the surface factors of epithelial lining contribute essentially to shaping the pulmonary susceptibility to influenza virus since there is no difference of the susceptibility of pulmonary cells to influenza virus between the two above animals' species.
  Vestnik Rossiĭskoĭ akademii meditsinskikh nauk / Rossiĭskaia akademiia meditsinskikh nauk 02/2004;
• Article: [Submicroscopic characteristics of Marburg virus and its mini genome analog replication in cell cultures].

  T B Cheusova, S Becker, E Muehlberger, E I Riabchikova
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  ABSTRACT: Marburg virus (Filoviridae) causes severe hemorrhagic fevers in humans and some lower primates with high mortality. The virus genome is formed by a single strand RNA of negative polarity, coding for seven structural proteins. We studied the ultrastructure of Marburg virus replicative cycle and replication of its minigenome RNA (coding for the terminal areas of the genome) in the presence of helper virus in VERO fibroblastoid cell culture and epithelioid MDCK cell culture. Ultrastructural parameters of Marburg virus multiplication in these cell cultures are virtually the same. The virus nucleocapsid assembly is performed on the outer side of EPR membrane and is not associated with preliminary accumulation of the precursor material. Virions form by budding on plasmalemma and are located on the entire surface in Vero cells and only on the basolateral surface of MDCK cells. Replication of minigenome analog of marburg virus is associated with impairment of the helper virus morphogenesis and formation of spherical pseudoviral particles.
  Molekuliarnaia genetika, mikrobiologiia i virusologiia 02/2002;
• Article: [Preclinical studies of the anticancer adenovirus cancerolysin preparation].

  G V Vdovichenko, V A Petrishchenko, A A Sergeev, I I Kim, O E Fatiukhina, L N Shishkina, M P Bogriantseva, I V Pliasunov, V A Sviatchenko, N N Kiselev, T D Kolokol'tsova, E I Riabchikova, A N Sergeev, S V Netesov
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  ABSTRACT: The anticancer drug Cancerolysin has been developed, by using the mutant Adel2 variant of human adenovirus serotype 5 designed at the State Research Center of Virology and Biotechnology. Cancerolysin possesses a high degree of replication activity for complementary cells 293 and p53-deficient tumor cells and, at the same time, has significant replication limitations in normal human cells. Preclinical studies of the drug on laboratory animals (mice, rabbits, guinea pigs) have demonstrated its harmlessness and safety. When stored at -40 and -70 degrees C, the drug showed no significant activity throughout the control observational period (1 year).
  Voprosy virusologii 51(6):39-42.
• Article: [Reproduction of cowpox virus strain EP-2 isolated from an elephant in primary fibroblast cultures and chorion-allantoic chick embryos].

  I V Vinogradov, G V Kochneva, S N Shchelkunov, E I Riabchikova
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  ABSTRACT: Electron microscopy was used to study the reproduction of cowpox virus strain EP-2 in the cells of a primary fibroblast cultures (PFC) and chorion-allantoic membrane (CAM) of chick embryos (CE). The sequential stages of viral morphogenesis and the structure of A-type inclusions were described. The parameters of viral reproduction in PFC and CE CAM were compared. The formation of crystalloid tubular structures in PFC, unusual electron dense inclusions in the cells of CE CAN, and different variants of A-type inclusions in the cells of a pock was found. The histological and ultrastructural characteristics of pocks in CE CAM are described.
  Voprosy virusologii 51(2):44-8.
• Article: [Isolation and characterization of bovine diarrhea viral isolates].

  A G Glotov, T I Glotova, E I Riabchikova, A N Sergeev
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  ABSTRACT: The paper presents the results of a study of the antigenic and electromicroscopic characteristics of 3 bovine viral diarrhea isolates from cattle in Siberia. All the isolates were antigenically related to the reference strain BK-1 and closely interrelated: their affinity was in the range of 92.2 to 96.4%. The aerosolic administration of the isolate of TM from the sick calf lung into 2 seronegative (4-6-month-old) calves caused the characteristic sings of acute respiratory disease with short diarrhea.
  Voprosy virusologii 51(1):42-5.
• Article: [Intranasal infection in mice inoculated with cowpox virus strain EP-2 isolated from the elephant].

  I V Vinogradov, G V Kochneva, E M Malkova, S N Shchelkunov, E I Riabchikova
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  ABSTRACT: The specific features of reproduction of EP-2 strain of cowpox virus (CPV) were studied in intranasally infected BALC/C mice by light and electron microscopy. Virus replication was found in the ciliated, intercalary, basal, and goblet cells (the nasal respiratory area), basal and supporting cells (the nasal olfactory area), ciliated, intercalary, goblet cells (the tracheal and bronchial epithelium), and collagen-producing, Schwann's, endothelial, smooth muscle, and adventitial cells. It has been shown that the CPV strain EP-2 locally replicates in the nasal cavity, trachea, and large bronchi and that there is no generalized infection.
  Voprosy virusologii 50(4):37-42.
• Article: [An experimental infection caused by the EP-2 strain of cowpox virus in mice of different ages].

  I V Vinogradov, G V Kochneva, E M Malkova, S N Shchelkunov, E I Riabchikova
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  ABSTRACT: The specificity of lethal infection was studied in noninbred white mice (age--15 to 20 and 25 to 30 days) infected intraperitoneally with the EP-2 strain of cowpox virus (CPV) in doses 10(5), 10(6) and 10(7) PFU. The virus caused the lethal infection in the 15-20-day mice; while the 25-30-day mice remained healthy and survived. Virologic, immunologic-and-histochemical and electron-microscopy examinations of the 15-20-day mice revealed a replication of the EP-2 strain in tissues bordering on the virus introduction area; there was no generalization of infection. The virus replicated first in the mesothelium cells, and after that, in fibroblasts as well as in the endothelial, fatty, adventicial, cross-striated and muscle cells and in myosatellites.
  Voprosy virusologii 48(5):34-8.
• Article: [Construction of recombinant variants or orthopoxviruses by inserting foreign genes into intragenic region of viral genome].

  O I Serpinskiĭ, G V Kochneva, I Kh Urmanov, G F Sivolobova, E I Riabchikova
  Molekuliarnaia biologiia 30(5):1055-65.
• Article: [Course of infection in white rats, infected with Venezuelan equine encephalomyelitis virus by a respiratory route].

  L E Bulychev, A N Sergeev, A B Ryzhikov, N V Tkacheva, E I Riabchikova
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  ABSTRACT: Time course of accumulation of Venezuelan equine encephalomyelitis virus (strain Trinidad) in the organism of white rats after respiratory infection was studied. Electronmicroscopic examination of the organs was carried out over the course of the disease. The virus was for the first time detected in the lungs of animals after aerogenous infection and in the olfactory area of the nasal cavity of animals infected by applying the virus into the nasal mucosa. Disease development in aerosol-infected rats was characterized by viremia and infection of the hemopoietic and lymphomyeloid systems, particularly of the thymus and bifurcation lymph nodes, as well as of the olfactory system and brain. At the late stage of infection the concentration of the agent in all the examined organs was below the detection threshold, and neutralizing antibodies were detected in the blood serum.
  Voprosy virusologii 40(2):79-82.
• Article: [Course of infection in guinea pigs, infected aerogenically by Venezuelan equine encephalomyelitis virus].

  L E Bulychev, A N Sergeev, A B Ryzhikov, N V Tkacheva, E I Riabchikova
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  ABSTRACT: The time course of virus accumulation was studied in guinea pigs aerogenously infected with Venezuelan equine encephalomyelitis virus, strain Trinidad. At first the agent was isolated from the lungs. The course of infection was characterized by viremia and infection of some organs and tissues of the lymphoid, hemopoietic, and central nervous systems, specifically, of the tonsils, lymph nodes, spleen, liver, bone marrow, some sections of the olfactory tract, and brain.
  Voprosy virusologii 40(3):122-4.
• Article: [The study of orthopoxvirus genes for Kelch-like proteins. II. Construction of cowpox virus variants with targeted gene deletions].

  I V Kolosova, S V Seregin, G V Kochneva, E I Riabchikova, E V Bessmel'tseva, I N Babkina, T E Solenova, I V Babkin, S N Shchelkunov
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  ABSTRACT: Integrative plasmids p delta C, p delta D, and p delta G were designed to contain a selective marker beyond the region of homology to virus DNA and to allow construction of recombinant cowpox viruses (CPV) that lack C18L, D11L, or G3L coding for kelch-like proteins. CPV mutants lacking one (C18L, D11L, or G3L), two (D11L/G3L or C18L/D11L), or three (D11L/G3L/C18L, that is, all) kelch-like protein genes of the left variable region of the virus genome were obtained. Impaired reproduction was observed for the triple mutant. Pocks produced by the triple mutant and the original virus differed in size and morphology. In addition, the two CPV variants differed in destructive changes caused in the chorioallantoic membrane of chicken embryos.
  Molekuliarnaia biologiia 37(4):585-94.
• Article: [The biotype and genetic characteristics of an isolate of the cowpox virus causing infection in a child].

  S S Marennikova, P V Gashnikov, O A Zhukova, E I Riabchikova, V V Strel'tsov, O I Riazankina, E V Chekunova, N N Ianova, S N Shchelkunov
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  ABSTRACT: A virus, identified as cowpox virus by its biological properties and the results of the analysis of its DNA, was isolated from a sick 4-year-old child with a clinical picture of pox, though having had no contacts with known natural carriers of the causative agent of this infection. At the same time the isolated virus was found to differ from the reference strain, as well as from other isolates of vaccinia virus by some biological markers (and in particular by the structure of cytoplasmic inclusions of type A) and by the restriction profile of DNA. The Hind III maps indicating the location of restriction sites made it possible to localize the genome differences established in this study. The specific feature of this case was the previous close contact of the child with a mole which was probably the source of infection.
  Zhurnal mikrobiologii, epidemiologii, i immunobiologii
• Article: [Comparative study of the morphology and antigenic properties of recombinant analogs of a Marburg virus nucleoprotein].

  A V Kachko, T B Cheusova, A V Sorokin, E I Kazachinskaia, I O Cheshenko, E F Belanov, A A Bukreev, A V Ivanova, I A Razumov, E I Riabchikova, S V Netesov
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  ABSTRACT: The full-length gene for Marburg virus (MV) nucleoprotein (NP) was cloned in prokaryotic pQE32 under the control of the T5 promoter and in eukaryotic pTM1 under the control of the promoter for T7 RNA polymerase. Recombinant NP was synthesized in Escherichia coli and in human kidney cell line 293 cotransfected with recombinant vaccinia virus vTF7-3 expressing T7 RNA polymerase. On evidence of electron microscopy with immune detection, recombinant NP formed tubules of two types in E. coli and of a single type in cell line 293. ELISA and immunoblotting with polyclonal and monoclonal antibodies revealed common antigenic determinants in recombinant NP and natural MV NP.
  Molekuliarnaia biologiia 35(3):492-9.
• Article: [Submicroscopic changes in the chorion-allantois of chick embryos infected with vaccinia virus].

  E I Riabchikova, V V Strel'tsov, V S Petrov
  Voprosy virusologii 35(6):506-9.