Triantafyllos A Albanis

University of Ioannina, Yannina, Epirus, Greece

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Publications (119)299.79 Total impact

  • Nikolaos P Petridis, Vasilios A Sakkas, Triantafyllos A Albanis
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    ABSTRACT: A dispersive suspended microextraction (DSME) method coupled with gas chromatography-mass spectrometry (GC-MS) was developed and validated for the simultaneous determination of ten polycyclic aromatic hydrocarbons in real water samples. The optimization of the method was achieved with a 2(7-4) Plackett-Burman design, while the significant factors were optimized using a central composite design (CCD). The parameters that were studied included the sample volume, organic solvent volume, extraction time, restoration time and organic solvent. The optimum experimental conditions for the proposed method comprised 4.3mL of the water sample, 93μL of toluene as the extraction solvent, a 104-s extraction time and a 10-min restoration time. The recoveries varied from 70 to 111%. Chrysene was the least recovered compound, while anthracene displayed the highest extraction efficiency. The analytical method (DSME) was shown to be linear (R(2)>0.993) over the studied range of concentrations, exhibiting satisfactory precision (RSD%<10.6%) and reaching limits of detection between 8 and 46ngL(-1).
    Journal of Chromatography A 06/2014; · 4.61 Impact Factor
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    ABSTRACT: A rapid magnetic solid-phase extraction (MSPE) is proposed based on C18-functionalized magnetic silica nanoparticles as sorbents, for the determination of endocrine disruptors - 20 organochlorine pesticides and 6 polychlorinated biphenyls - in milk samples. Magnetic nanoparticles are characterized by several techniques, such as Scanning Electron Microscopy, X-Ray diffraction, Brunauer-Emmett-Teller and Fourier transform-infrared. The MSPE is performed by dispersion of the Fe3O4@SiO2@C18 nanoparticles in milk samples with sonication, after protein precipitation. Then, the sorbent is collected by applying an external magnetic field and the analytes are desorbed by n-hexane. Several parameters affecting the extraction efficiency of target analytes by the magnetic nanoparticles are investigated, including washing and elution solvents, amount of sorbents, time of extraction and elution, sample and elution solvent volume. The proposed method is optimized by means of experimental design and response surface methodology. When coupled with gas chromatography-mass spectrometry detection and under optimum extraction conditions, average recoveries of target analytes are found to be in the range of 79% to 116%. The proposed MSPE-GC-MS analytical method has a linear calibration curve for all target analytes with coefficients of determination to range from 0.9950 to 0.9999. The limits of quantification are found to be between 0.2 and 1μg/L ensuring compliance with the maximum residue limits established by European Commission and Codex Alimentarius, for OCPs and PCBs residues in milk. The proposed method is applied to the determination of target analytes in milk samples from local markets.
    Journal of chromatography. A. 05/2014;
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    ABSTRACT: Organochlorine (OC) pesticides and polychlorinated biphenyls (PCB) are compounds characterized as persistent organic pollutants (POP) in the environment. These compounds are monitored globally since they enter the human body and accumulate in tissues, resulting in consequent adverse effects. In this study concentrations of selected OC compounds were determined in human autopsy lungs from Epirus, a relatively restricted region in northwestern Greece. This is the first epidemiologic study from Greece reporting on monitoring of environmental pollutants in human autopsy material. Thirty lungs collected from that number of autopsy cases were analyzed: 19 males and 11 females. The age range was 14-91 yr (mean ± SD = 61.8 ± 22.5 yr). Twenty-two cases (73%) were positive for at least one pollutant and eight cases were negative (27%). PCB were the most abundant class of contaminants, detected in 15 out of the 30 cases (50%). Dichlorodiphenyltrichloroethane (DDT) and hexachlorocyclohexanes (HCH) were second and third in abundance with 9 (30%) and 8 (27%) positive cases, respectively. The frequency of detection showed a tendency to increase with age of individuals. The patterns of OC found in human autopsy lungs were similar to those reported previously for other human specimens. Our results demonstrated a similar trend in contamination sources and distribution has occurred in western Greece as noted globally.
    Journal of Toxicology and Environmental Health Part A 01/2014; 77(13):767-75. · 1.73 Impact Factor
  • Christina I Kosma, Dimitra A Lambropoulou, Triantafyllos A Albanis
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    ABSTRACT: In the present work, an extensive study on the presence of eighteen pharmaceuticals and personal care products (PPCPs) in eight wastewater treatment plants (WWTPs) of Greece has been conducted. The study covered four sampling periods over 1-year, where samples (influents; effluents) from eight WWTPs of various cities in Greece were taken. All WWTPs investigated are equipped with conventional activated sludge treatment. A common pre-concentration step based on SPE was applied, followed by LC-UV/Vis-ESI-MS. Further confirmation of positive findings was accomplished by using LC coupled to a high resolution Orbitrap mass spectrometer. The results showed the occurrence of all target compounds in the wastewater samples with concentrations up to 96.65μg/L. Paracetamol, caffeine, trimethoprim, sulfamethoxazole, carbamazepine, diclofenac and salicylic acid were the dominant compounds, while tolfenamic acid, fenofibrate and simvastatin were the less frequently detected compounds with concentrations in effluents below the LOQ. The removal efficiencies showed that many WWTPs were unable to effectively remove most of the PPCPs investigated. Finally, the study provides an assessment of the environmental risk posed by their presence in wastewaters by means of the risk quotient (RQ). RQs were more than unity for various compounds in the effluents expressing possible threat for the aquatic environment. Triclosan was found to be the most critical compound in terms of contribution and environmental risk, concluding that it should be seriously considered as a candidate for regulatory monitoring and prioritization on a European scale on the basis of realistic PNECs. The results of the extensive monitoring study contributed to a better insight on PPCPs in Greece and their presence in influent and effluent wastewaters. Furthermore, the unequivocal identification of two transformation products of trimethoprim in real wastewaters by using the advantages of the LTQ Orbitrap capabilities provides information that should be taken into consideration in future PPCP monitoring studies in wastewaters.
    Science of The Total Environment 08/2013; 466-467C:421-438. · 3.16 Impact Factor
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    ABSTRACT: The marine calanoid copepod Acartia tonsa was exposed to methyltestosterone (MET, 1.6-126μgL(-1)), letrozole (LET, 10-1000μgL(-1)), triphenyltin chloride (TPT, 0.0014-0.0088μgL(-1) TPT-Sn) and fenarimol (FEN, 2.8-105μgL(-1)) for 21d covering a full life-cycle. All four compounds investigated are known to act as androgens in vertebrates. The digestive tract, musculature, nervous system, reproductive organs, gonad and accessory sexual glands were examined by light microscopy after routine staining and immune-labelling for detection of apoptosis and determination of proliferation activities. MET induced an inhibition of oogenesis, oocyte maturation and yolk formation, respectively, which was most pronounced at the lowest concentrations tested. In LET exposed males, spermatogenesis was enhanced with very prominent gamete stages; in some stages apoptosis occurred. The spermatophore was hypertrophied and displayed deformations. In females, LET induced a disorder of oogenesis and disturbances in yolk synthesis. TPT stimulated the male reproductive system at 0.0014 and 0.0035μg TPT-SnL(-1), whereas inhibiting effects were observed in the female gonad at 0.0088μg TPT-SnL(-1). In FEN exposed females proliferation of gametes was reduced and yolk formation showed irregular features at 2.8-105μgL(-1). In FEN exposed males an elevated proliferation activity was observed. No pathological alterations in other organ systems, e.g. the digestive tract including the hindgut acting as respiratory organ, the nervous system, or the musculature were seen. This indicates that the effects on gonads might be caused rather by disturbance of endocrine signalling or interference with hormone metabolism than by general toxicity.
    Chemosphere 05/2013; · 3.14 Impact Factor
  • M. Azharul Islam, Vasilios Sakkas, Triantafyllos A. Albanis
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    ABSTRACT: Removal of two organophosphorus pesticides bromophos methyl [BM: O, O- dimethyl - O - (2, 5-dichloro -4 bromophenyl) phosphorothioate] and quinalphos [QP: O, O-diethyl O-2-quinoxalinyl phosphorothioate] on used tea leaves were studied by batch equilibration method. Adsorption isotherms were conformed well to Langmuir for quinalphos and Freundlich equation for bromophos methyl. The kinetic data fitted well by the pseudo second order model for both pesticides. Box-Behnken design was successfully employed for experimental design and analysis of results. The interactions of pH, initial concentration and adsorbent dose on two pesticides adsorption by used tea leaves were investigated by this model. The optimum pH, initial concentration and adsorbent dose with their corresponding removal efficiency were found to be 7.88, 11.94 mg L-1, 0.37g and 100% for bromophos methyl respectively, for quinalphos 8.72, 6.44 mg L-1, 0.39g and 93.98% respectively. Keywords: Box-Behnken; quinalphos; bromophos methyl; Kinetics; used tea leaves
    04/2013;
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    ABSTRACT: Chemometric optimization tools were employed, such as experimental design and response surface methodology (RSM) to assess the efficiency of two advanced oxidation processes (AOPs): homogeneous Fenton (FeII/H2O2) and heterogeneous (TiO2) photocatalysis for the degradation of the artificial sweetener sucralose. The aqueous samples were irradiated under a variety of experimental conditions (pH, light intensity) and with different amounts of H2O2, Fe(II), TiO2. The use of RSM allowed fitting the optimal values of the parameters leading to the degradation of the contaminant. Also, a single polynomial expression modeling the reaction was obtained for both AOPs.The intermediates formed during the photocatalytic and photo-Fenton process were investigated and characterized by means of HPLC/HRMS. The photocatalysed transformation of sucralose proceeds through the formation of few (eight) products, involving four different pathways: hydroxylation of the molecule, oxidation of the alcohol function, dechlorination and the cleavage of glycoside bond. All the identified intermediates were easily degraded and within four hours of irradiation complete mineralization was achieved. A comparison with Photo-Fenton reaction was also reported.In addition Microtox bioassay (Vibrio fischeri) was employed in evaluating the ecotoxicity of solutions treated by heterogeneous photocatalysis. Results clearly demonstrate the efficiency of the photocatalytic process in the detoxification of the irradiated solutions.
    Applied Catalysis B Environmental 01/2013; · 5.83 Impact Factor
  • Ourania D Christopoulou, Vasilios A Sakkas, Triantafyllos A Albanis
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    ABSTRACT: A simple, fast, and inexpensive procedure for sample preparation based on matrix solid-phase dispersion was developed for the determination of Environmental Protection Agency 16 priority polycyclic aromatic hydrocarbons in indoor dust samples. Parameters that affect the extraction efficiency such as type of dispersant, elution solvent, and solvent volume were evaluated and optimized with the aid of experimental design and response surface methodology. Analysis was performed by HPLC coupled with UV-Vis diode array detector (UV-DAD). For verification, a GC coupled with a mass spectrometer in SIM mode was also applied. Recoveries obtained were from 53 to 120% for all target analytes with detection limits ranging from 0.2 to 10 ng/g and 0.2 to 2 ng/g for LC-UV-DAD and GC-MS, respectively. The optimized method was used for the analysis of 11 household dust samples collected from private houses.
    Journal of Separation Science 11/2012; · 2.59 Impact Factor
  • Theodora P Tsiallou, Vasilios A Sakkas, Triantafyllos A Albanis
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    ABSTRACT: A simple and green method based on dispersive liquid-liquid microextraction, mated to chemometrics and followed by mass spectrometric detection for the determination of suspected fragrance allergens in water samples is developed and assessed in this work. Volume of extraction and disperser solvent, pH, ionic strength, extraction time, sample volume, as well as centrifugation time were initially optimized in a fractional factorial design. The obtained significant factors were optimized by using a central composite design and the quadratic model between the dependent and the independent variables was built. The obtained optimal conditions were: aqueous sample of 3.8 mL, 100 μL chloroform, 1.40 mL acetone, 4 min centrifugation time, natural pH containing 5% (w/v) NaCl, and centrifugation speed 4000 rpm. Method proved to be linear over a wide range of concentration for all analytes with R(2) between 0.9807 and 0.9959. The repeatability and reproducibility of the proposed method, expressed as relative standard deviation, varied between 3-13% and 4-16%, respectively. The limits of detection ranged from 0.007 to 1.0 μg L(-1) . The recommended method was applied to water samples including baby bath as well as swimming pool water samples and was compared with a previously reported method.
    Journal of Separation Science 07/2012; 35(13):1659-66. · 2.59 Impact Factor
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    ABSTRACT: A matrix solid-phase dispersion (MSPD) method for the simultaneous determination of 20 organochlorine pesticides (OCPs) (aldrin, endrin, dieldrin, α-BHC, β-BHC, γ-BHC, δ-BHC, α-chlordane, γ-chlordane, p,p'-DDE, p,p'-DDT, p,p'-DDD, endosulfan I, endosulfan II, endosulfan sulfate, endrin aldehyde, heptachlor, heptachlor epoxide, endrin ketone and methoxychlor) and six polychlorinated biphenyl (PCB) congeners (PCB 28, 52, 101, 138, 153 and 180) in post-mortem human lung has been developed and validated. Response surface methodology (RSM) and desirability function were employed to optimize the extraction conditions of MSPD. Extraction was carried out using Florisil (2.0 g) as the sorbent material as well as clean-up adsorbent (1.5401 g), n-hexane:dichloromethane (11:89, v/v) as the eluting solvent (15.45 mL) and Na₂SO₄ (2.0 g) as dehydrating agent. Determination and quantification of OCPs and PCBs residues were carried out using a gas chromatograph equipped with an electron capture detector (GC-ECD). A mass spectrometric detector (GC-MS) in the selected ion monitoring (SIM) mode was also used for confirmation purposes. Method detection limits by GC-MS ranged from 0.42 to 0.87 ng g⁻¹ and 0.51 to 1.35 ng g⁻¹, for OCPs and PCBs, respectively. Lower detection limits were calculated for GC-ECD ranging between 0.15-0.30 ng g⁻¹ and 0.18-0.48 ng g⁻¹, respectively. Relative standard deviations did not exceed 9%. Analytes provided recoveries ranging from 65% to 106%. The proposed method was successfully applied to the analysis of lung tissues from six autopsy cases.
    Journal of Chromatography A 03/2012; 1227:1-9. · 4.61 Impact Factor
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    Chemosphere 01/2012; 86(2):216. · 3.14 Impact Factor
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    ABSTRACT: Olive mill wastewater (OMW) is one of the major and most challenging organic pollutants in olive oil production countries. However, the knowledge about the in-situ effects of olive mill wastewaters to lotic ecosystems and their benthic organisms is very limited. To resolve this, eight sampling sites were selected upstream and downstream the outflow of several olive mills to assess the spatial and temporal effects of OMW to stream macroinvertebrates and to ecological status of stream ecosystems. Biotic (macroinvertebrates) and abiotic (physicochemical, hydromorphological) data were monitored for two years thus following the biennial cycle of olive growth and production and hydrological variation (drought-wet years). The results of this study revealed the spatial and temporal structural deterioration of the aquatic community due to OMW pollution with consequent reduction of the river capacity for reducing the effects of polluting substances through internal mechanisms of self-purification. OMW, even highly diluted, had dramatic impacts on the aquatic fauna and to the ecological status of the receiving stream ecosystems. The organic load of the wastewater expressed as BOD(5), COD and TSS, substrate contamination (sewage bacteria) and distance from the mill outlet, were the most important factors affecting macroinvertebrate assemblages while the typology (i.e. slope, altitude) and hydrology of the stream site (i.e. mountainous-lowland) and the intensity and volume of the wastewater were the most important determinants of self-purification processes. As OMW are usually being discharged in small size streams that are not considered in the Water Framework Directive 2000/60/EC, there is a need for including such systems into monitoring and assessment schemes as they may significantly contribute to the pollution load of the river basin. Furthermore, guidelines to manage these wastes through technologies that minimise their environmental impact and lead to a sustainable use of resources are critical.
    Water Research 12/2011; 45(19):6334-46. · 4.66 Impact Factor
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    ABSTRACT: The toxicity and the biochemical effects of olive mill wastewater and citrus processing wastewater were evaluated using acute toxicity bioassays (Gammarus pulex and Hydropsyche peristerica) and biochemical biomarkers (acetylcholinesterase [AChE] and glutathione S-transferase [GST]). The bioassays indicated toxic properties of olive mill and citrus processing wastewaters. The 24 h LC(50) values of olive mill wastewater ranged from 2.64% to 3.36% for G. pulex and 3.62% to 3.88% for H. peristerica, while the LC(50) of citrus processing wastewater was 25.26% for G. pulex and 17.16% for H. peristerica. Based on a five-class hazard classification system applied for wastewaters discharged into the aquatic environment, olive mill wastewater and citrus processing wastewater were classified as highly toxic and toxic, respectively. Results of the biochemical biomarkers showed that both agroindustrial effluents at increasing sublethal wastewater concentrations could cause inhibition of the AChE and induction of the GST activities. These first results showed that both species as well as their AChE and GST activities have the potential to be used as indicators and biomarkers for assessing olive mill and citrus processing wastewaters quality.
    Environmental Toxicology 11/2011; 26(6):669-76. · 2.71 Impact Factor
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    ABSTRACT: In this study, nutrients, trace metals and priority pesticide compounds were investigated for the first time in water and sediment samples in streams of the Evrotas River basin (S.E. Greece) from 2006 to 2008. The most important sources of contamination were from the entry of pesticides and nutrients into surface waters and sediments as a result of the intensive agricultural activity as well as from the uncontrolled disposal of olive mill and citrus processing wastewaters. Aquatic risk assessment revealed that all insecticides detected showed high risk, suggesting adverse effects on the stream biota. Among the metals analyzed, Cr, Ni and Ba presented the highest concentrations in sediments, however, due to natural geological processes. Multivariate statistical techniques applied for data compression, exploration and interpretation proved to be useful tools for identifying the most critical pollutants affecting the surface water quality. The findings of this study suggest that the inclusion of streams with small catchment areas into WFD monitoring and assessment programs is essential, especially those of the Mediterranean region.
    Journal of Environmental Monitoring 09/2011; 13(11):3064-74. · 2.09 Impact Factor
  • 16th International Symposium on Environmental Pollution and its impact on Life in the Mediterranean region; 09/2011
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    ABSTRACT: In the National Park of Dadia-Lefkimi-Soufli Forest (Dadia NP, Greece), seven "target" PCBs and 16 organochlorine pesticides (OCs) were analysed in blood samples of cinereous vultures (Aegypius monachus) and Eurasian griffon vultures (Gyps fulvus). PCB congeners 138, 153 and 180 predominated in both species' blood samples. In both species, no differences were detected in congener levels between successive age classes, but in cinereous vulture, there were significant differences between adult and nestling in levels of PCB 28, 52, 101, 118 and between nestling and immature in levels of PCB 101. Regarding pesticides, p,p'-DDE dominated in both vultures followed by β-HCH, lindane and endosulfan sulphate, but ∑OCs were higher in griffon vulture. Significant differences were detected only between nestling and sub-adult cinereous vultures in heptachlor levels and between nestling and adult in p,p'-DDT. The origin of pollutants differs between the two vulture species and pollution patterns may not reflect those at Dadia NP.
    Environmental Monitoring and Assessment 02/2011; 183(1-4):259-71. · 1.68 Impact Factor
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    ABSTRACT: In order to exploit the efficiency of titania suspensions in photocatalysis for the degradation of the herbicide flufenacet, chemometric optimization tools were employed, such as response surface methodology and experimental design. The aqueous samples were irradiated under a variety of experimental conditions with different amounts of catalyst (TiO2), electron acceptor (H2O2) as well as pH. Results indicated that the degradation efficiency of the herbicide in the experimental domain investigated was mainly affected by the concentration of H2O2, followed by TiO2, pH, as well as their interaction effects.Additionally, the phototransformation products formed during the photocatalytic process were investigated and characterized by means of HPLC/HRMS. The photocatalysed transformation of flufenacet proceeds through the formation of thirty-two (32) products, involving reactions of mono- and di-hydroxylation, dealkylation, detachment of the thiadiazole ring, defluorination on the benzene ring followed by the detachment of the latter ring. The thiadiazole ring appears to be involved in the process to a lesser extent and only as a secondary path. The measurement of acute toxicity, evaluated using the Vibrio fischeri bacteria test, showed that the transformation of flufenacet proceeds through the formation of compounds more toxic than the parent molecule. Although the identified intermediates were easily degraded and within two hours of irradiation were completely disappeared, mineralization was much slower and complete formation of CO2 and inorganic constituents was only achieved after 24h of irradiation.
    Applied Catalysis B Environmental 01/2011; · 5.83 Impact Factor
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    ABSTRACT: The main objective of this paper is to provide data on the Danube River Delta Biosphere (Romania) aquatic ecosystem quality and Vardar – Axios River Valley (FYR Macedonia – Greece) on comparison basis from on the long-term analytical investigation for specific indicators. In particular this paper is focused on the quality assessment of surface water, sediment and biota of the Danube Delta Biosphere Reservation. Information is presented on water and sediment quality variations in heavy metals over a seven-year period (2003 and 2009), from April to October. Samples have been collected monthly from two different locations (Murghiol and Uzlina) situated on St. Gheorghe branch. The presence of some toxic metals was noticed in surface waters and sediments. In both locations in different periods of time high concentrations of Fe, Mn, heptachlor, Lindane, PCBs, phenols, mineral oil in surface water represent an acute problem for the inhabitants of the Danube Delta which use water for household consumption directly from the Danube, without any treatment. This presents a series of disadvantages, such as a high risk of disease for the population, mostly for children and old people. The evolution of the pollution levels of Danube waters was compared with that of Axios/Vardar River, another major transboundary river in the Balkan area. KeywordsDanube Delta-Axios – Vardar River-ecosystem-metals-Pollution-drinking water-health-sediment-benthic macro invertebrates
    12/2010: pages 257-286;
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    ABSTRACT: The multi-residue trace-level determination of six pesticides (diazinon, dimethoate, chlorpyrifos, vinclozolin, fenthion and quinalphos) in wine samples, after their single-drop microextraction (SDME) is presented herein. The extraction procedure was optimized using the multivariate optimization approach following a two-stage process. The first screening experimental design brought out the significant parameters and was followed by a central composite design (CCD) experiment, which revealed the simultaneous effect of the significant factors affecting the SDME process. High level of linearity for all target analytes was recorded with r(2) ranging between 0.9978 and 0.9999 while repeatability (intra-day) and reproducibility (inter-day) varied from 5.6% to 7.4% and 4.9% to 12.5%, respectively. Limits of detection (LODs) and limits of quantification (LOQs) were found to range in the low microg L(-1) level. In general, the developed methodology presented simplicity and enhanced sensitivity, rendering it appropriate for routine wine screening purposes.
    Talanta 09/2010; 82(4):1286-91. · 3.50 Impact Factor
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    ABSTRACT: Activated carbons (ACs) can be used not only for liquid but also for vapour phase applications, such as water treatment, deodorisation, gas purification and air treatment. In the present study, activated carbons produced from agricultural residues (olive kernel, corn cobs, rapeseed stalks and soya stalks) via physical steam activation were tested for the removal of Bromopropylate (BP) from water. For the characterization of the activated carbons ICP, SEM, FTIR and XRD analyses were performed. Adsorption kinetics and equilibrium isotherms were investigated for all biomass activated carbons in aqueous solutions. Experimental data of BP adsorption have fitted best to the pseudo 2nd-order kinetic model and Langmuir isotherm. The study resulted that corn cobs showed better adsorption capacity than the other biomass ACs. Comparison among ACs from biomass and commercial ones (F400 and Norit GL50) revealed that the first can be equally effective for the removal of BP from water with the latter.
    Chemosphere 09/2010; 80(11):1328-36. · 3.14 Impact Factor

Publication Stats

2k Citations
299.79 Total Impact Points

Institutions

  • 1988–2014
    • University of Ioannina
      • • Laboratory of Analytical Chemistry
      • • Department of Chemistry
      Yannina, Epirus, Greece
  • 2011
    • Hellenic Centre for Marine Research
      Ανάβυσσος, Attica, Greece
  • 2007–2010
    • Aristotle University of Thessaloniki
      • • Department of Chemical Engineering
      • • Laboratory of Enviromental Chemistry
      Thessaloníki, Kentriki Makedonia, Greece
  • 2006
    • General Chemical State Laboratory of Greece
      Athínai, Attica, Greece
  • 2004
    • Università degli Studi della Basilicata
      • Department of Sciences
      Potenza, Basilicate, Italy
    • Hanseo University
      Kōjō, South Chungcheong, South Korea