Luqi Huang

Chinese Academy of Medical Sciences, Peping, Beijing, China

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Publications (150)221.02 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Molecularly imprinted polymers (MIPs) with high selectivity and affinity to glabridin were designed based on the screening results of the library of non-imprinted polymers (NIPs). The NIP library contained 48 polymers that were polymerized with the combinations of different functional monomers, cross-linkers, and porogenic solvents. The distribution coefficient (k) values were used to estimate the affinity of NIPs to glabridin. The corresponding MIPs of the best three NIPs were prepared. After evaluating the imprinting effects and selectivity of the three MIPs, the performance of the best MIP as solid phase extraction sorbent was investigated. Glabridin with percent recovery of 83 was obtained from the extract of Glycyrrhiza glabra L. (G. glabra L.) residues by molecularly imprinted solid phase extraction (MISPE). Thus, this material can be successfully used for the extraction and purification of glabridin from G. glabra L. residues.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 06/2014; 965C:1-6. · 2.78 Impact Factor
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    ABSTRACT: This study was performed to compare the contents of total phenolics, total flavonoids, and total triterpenes between peel and flesh of ten different pear cultivars. The monomeric compounds were analyzed by HPLC, their antioxidant and anti-inflammatory activities were also measured. Peel and flesh from Yaguang, Hongpi, Qingpi and Guifei varieties contained relatively more total phenolic, total flavonoids and total triterpene, and showed stronger antioxidant and anti-inflammatory activities, while Lvbaoshi and Youran appeared to be weakest among them. All the chemical components found in the pear peel were approximately 6-20 times higher than those in the flesh of pear. For the monomeric compounds, arbutin, oleanolic acid, ursolic acid, chlorogenic acid, epicatechin, and rutin were the dominant components contained in the ten pear cultivars both in peel and in flesh. All of the analyses suggested that the peel of pear might be an excellent polyphenol and triterpenes source.
    Food Chemistry 06/2014; 152C:531-538. · 3.33 Impact Factor
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    ABSTRACT: Molecular imprinted polymers (MIPs) with high selectivity and affinity to gastrodin in water were designed using allyl 2,3,4,6-tetra-O-acetyl-glucopyranoside (TAGL) and 1,2,3,4,5-pentafluoro-6-vinylbenzene (PFVB) as novel functional monomers. Binding characterization of pre-polymerization complexes was researched by nuclear magnetic resonance (NMR) and the MIPs were characterized by scanning electron microscopy (SEM). The properties involving adsorption isotherm, adsorption kinetics and selective recognition capacity were evaluated. The MIPs/TAGL exhibited good site accessibility in which it only took 30min to achieve adsorption equilibrium and highly selective recognition for the template. Furthermore, the performance of the MIPs/TAGL as solid phase extraction material was investigated in detail and hot water at 50°C served as the eluting solvent. Pure gastrodin with the recovery of 76.6% was obtained from the aqueous extract of Gastrodia elata roots.
    Journal of Chromatography A 03/2014; · 4.61 Impact Factor
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    ABSTRACT: Plant natural products have been co-opted for millennia by humans for various uses such as flavor, fragrances, and medicines. These compounds often are only produced in relatively low amounts and are difficult to chemically synthesize, limiting access. While elucidation of the underlying biosynthetic processes might help alleviate these issues (e.g., via metabolic engineering), investigation of this is hindered by the low levels of relevant gene expression and expansion of the corresponding enzymatic gene families. However, the often-inducible nature of such metabolic processes enables selection of those genes whose expression pattern indicates a role in production of the targeted natural product. Here, we combine metabolomics and transcriptomics to investigate the inducible biosynthesis of the bioactive diterpenoid tanshinones from the Chinese medicinal herb, Salvia miltiorrhiza (Danshen). Untargeted metabolomics investigation of elicited hairy root cultures indicated that tanshinone production was a dominant component of the metabolic response, increasing at later time points. A transcriptomic approach was applied to not only define a comprehensive transcriptome (comprised of 20,972 non-redundant genes), but also its response to induction, revealing 6,358 genes that exhibited differential expression, with significant enrichment for up-regulation of genes involved in stress, stimulus and immune response processes. Consistent with our metabolomics analysis, there appears to be a slower but more sustained increased in transcript levels of known genes from diterpenoid and, more specifically, tanshinone biosynthesis. Among the co-regulated genes were 70 transcription factors and 8 cytochromes P450, providing targets for future investigation. Our results indicate a biphasic response of Danshen terpenoid metabolism to elicitation, with early induction of sesqui- and tri- terpenoid biosynthesis, followed by later and more sustained production of the diterpenoid tanshinones. Our data provides a firm foundation for further elucidation of tanshinone and other inducible natural product metabolism in Danshen.
    BMC Genomics 01/2014; 15(1):73. · 4.40 Impact Factor
  • Food Hydrocolloids 01/2014; 39:286–294. · 3.49 Impact Factor
  • Food Hydrocolloids 01/2014; 40:196–202. · 3.49 Impact Factor
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    ABSTRACT: In this work, a rapid (within 4-5 h), sensitive and visible new method for assessing botanic origin is developed by combining loop-mediated isothermal amplification with cationic conjugated polymers. The two Chinese medicinal materials (Jin-Yin-Hua and Shan-Yin-Hua) with similar morphology and chemical composition were clearly distinguished by gene SNP genotyping assays. The identification of plant species in Patented Chinese drugs containing Lonicera buds is successfully performed using this detection system. The method is also robust enough to be used in high-throughput screening. This new method is very helpful to identify herbal materials, and is beneficial for detecting safety and quality of botanic products.
    Scientific reports. 01/2014; 4:5395.
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    ABSTRACT: 4-Coumarate:CoA ligases (4CLs) are a group of essential enzymes involved in the pathway of phenylpropanoid-derived compound metabolisms; however it is still difficult to identify orthologs and paralogs of these important enzymes just based on sequence similarity of the conserved domains. Using sequence data of 20 plant species from the public databases and sequences from Lonicera japonica, we define 1252 adenosine monophosphate (AMP)-dependent synthetase/ligase sequences and classify them into three phylogenetic clades. 4CLs are in one of the four subgroups, according to their partitioning, with known proteins characterized in A. thaliana and Oryza sativa. We also defined 184 non-redundant sequences that encode proteins containing the GEICIRG motif and the taxonomic distribution of these GEICIRG-containing proteins suggests unique catalytic activities in plants. We further analyzed their transcription levels in L. japonica and L. japonica. var. chinensis flowers and chose the highest expressed genes representing the subgroups for structure and binding site predictions. Coupled with liquid chromatography-mass spectrometry (LC-MS) analysis of the L. japonica flowers, the structural study on putative substrate binding amino acid residues, ferulate, and 4-coumaric acid of the conserved binding-site of LJ4CL1 leads to a conclusion that this highly expressed protein group in the flowers may process 4-coumarate that represents 90% of the known phenylpropanoid-derived compounds. The activity of purified crude LJ4CL1 protein was analyzed using 4-coumarate as template and high activity indicating that 4-coumarate is one of the substrates of LJ4CL1.
    International Journal of Molecular Sciences 01/2014; 15(2):2386-99. · 2.46 Impact Factor
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    ABSTRACT: Ginsenosides are the primary bioactive components of ginseng, which is a popular medicinal plant that exhibits diverse pharmacological activities. Protopanaxadiol, protopanaxatriol and oleanolic acid are three basic aglycons of ginsenosides. Producing aglycons of ginsenosides in Saccharomyces cerevisiae was realized in this work and provides an alternative route compared to traditional extraction methods. Synthetic pathways of these three aglycons were constructed in S. cerevisiae by introducing β-amyrin synthase, oleanolic acid synthase, dammarenediol-II synthase, protopanaxadiol synthase, protopanaxatriol synthase and NADPH-cytochrome P450 reductase from different plants. In addition, a truncated 3-hydroxy-3-methylglutaryl-CoA reductase, squalene synthase and 2,3-oxidosqualene synthase genes were overexpressed to increase the precursor supply for improving aglycon production. Strain GY-1 was obtained, which produced 17.2 mg/L protopanaxadiol, 15.9 mg/L protopanaxatriol and 21.4 mg/L oleanolic acid. The yeast strains engineered in this work can serve as the basis for creating an alternative way for producing ginsenosides in place of extractions from plant sources.
    Scientific Reports 01/2014; 4:3698. · 2.93 Impact Factor
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    ABSTRACT: Molecularly imprinted polymers (MIPs) with high selectivity and affinity to glabridin were designed based on the screening results of the library of non-imprinted polymers (NIPs). The NIP library contained 48 polymers that were polymerized with the combinations of different functional monomers, cross-linkers, and porogenic solvents. The distribution coefficient (k) values were used to estimate the affinity of NIPs to glabridin. The corresponding MIPs of the best three NIPs were prepared. After evaluating the imprinting effects and selectivity of the three MIPs, the performance of the best MIP as solid phase extraction sorbent was investigated. Glabridin with percent recovery of 83 was obtained from the extract of Glycyrrhiza glabra L. (G. glabra L.) residues by molecularly imprinted solid phase extraction (MISPE). Thus, this material can be successfully used for the extraction and purification of glabridin from G. glabra L. residues.
    Journal of Chromatography B. 01/2014; 965:1–6.
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    ABSTRACT: This study analyzed the effects of ecological factors on secondary metabolites of Scutellaria baicalensis using two sources: 92 individual roots of S. baicalensis from all over China, and secondary metabolites, medicinal materials and inorganic element contents obtained from the testing of 92 S. baicalensis rhizosphere soil samples. The study used environmental data from the Genuine Medicinal Material Spatial Analysis Database. Most of the chemical constituents of S. baicalensis were negatively correlated to latitude and positively correlated to temperature; generally, the contents of 21 chemical constituents were higher at low latitudes than that at high latitudes. By gradual regression analysis, it was found that the content of baicalin in S. baicalensis was negatively correlated to latitude and generally the content of inorganic elements in soil was excessively high (excluding Mg and Ca), which has a negative effect on the accumulation of chemical constituents in S. baicalensis. Based on the cluster analysis of 21 constituents, S. baicalensis from different places of origin was divided into two groups, and S. baicalensis was not genuine only in a specific small region. Within the zone from Chifeng, Inner Mongolia to Taibai, Shaanxi is suitable for accumulation of secondary metabolites of S. baicalensis and such a zone represents a suitable distribution and potential genuine producing area.
    Science China. Life sciences 11/2013; 56(11):1047-56. · 2.02 Impact Factor
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    ABSTRACT: Ginsenosides are the primary bioactive components of ginseng, which is a popular medicinal herb and exhibits diverse pharmacological activities. Protopanaxadiol is the aglycon of several dammarane-type ginsenosides, which also has anticancer activity. For microbial production of protopanaxadiol, dammarenediol-II synthase and protopanaxadiol synthase genes of Panax ginseng, together with a NADPH-cytochrome P450 reductase gene of Arabidopsis thaliana, were introduced into Saccharomyces cerevisiae, resulting in production of 0.05mg/g DCW protopanaxadiol. Increasing squalene and 2,3-oxidosqualene supplies through overexpressing truncated 3-hydroxyl-3-methylglutaryl-CoA reductase, farnesyl diphosphate synthase, squalene synthase and 2,3-oxidosqualene synthase genes, together with increasing protopanaxadiol synthase activity through codon optimization, led to 262-fold increase of protopanaxadiol production. Finally, using two-phase extractive fermentation resulted in production of 8.40mg/g DCW protopanaxadiol (1189mg/L), together with 10.94mg/g DCW dammarenediol-II (1548mg/L). The yeast strains engineered in this work can serve as the basis for creating an alternative way for production of ginsenosides in place of extraction from plant sources.
    Metabolic Engineering 10/2013; · 6.86 Impact Factor
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    ABSTRACT: Tanshinones are a group of bioactive abietane-type norditerpenoid quinone compounds in Salvia miltiorrhiza. Copalyldiphosphate synthase of S. miltiorrhiza (SmCPS) is the first key enzyme in tanshinone biosynthesis from the universal diterpene precursor geranylgeranyl diphosphate. Hairy roots of S. miltiorrhiza were transformed with Agrobacterium rhizogenes carrying an RNA interference (RNAi) construct designed to silence SmCPS, and we examined the resulting SmCPS expression and tanshinone accumulation. In SmCPS-RNAi hairy roots, the transcript level of SmCPS was reduced to 26 % while the dihydrotanshinone I and cryptotanshinone levels were decreased by 53 and 38 % compared to those of the vector control hairy roots; tanshinone IIA was not detected. Therefore, the decreased expression of SmCPS caused a decrease in tanshinone levels which verifies that SmCPS is a key enzyme for tanshinone biosynthesis in S. miltiorrhiza.
    Biotechnology Letters 09/2013; · 1.85 Impact Factor
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    ABSTRACT: The main therapeutic concept in Traditional Chinese Medicine (TCM) is herb formula, which treats various diseases via potential herb interactions to maximize the efficacy and minimize the adverse effects. However, the combination principle of herb formula still remains a mystery due to the lack of appropriate methods. A systems pharmacology method integrating the pharmacokinetic analysis, drug targeting, and drug-target-disease network is developed to dissect this rule embedded in the herbal formula. All these are exemplified by a representative TCM formula, Ma-huang Decoction, made up of four botanic herbs. Based on the deep investigation of the function and compatibility of each herb, in a molecular/systems level, we demonstrate the different pharmacological roles that each herb might play in the prescription. By the way of enhancing the bioavailability and/or making the pharmacological synergy among different herbs, the four herbs effectively combine together to be suitable for treating diseases. The present work lays foundations for a more comprehensive understanding of the combination rule of TCM, which might also be beneficial to drug development and applications.
    Journal of ethnopharmacology 09/2013; · 2.32 Impact Factor
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    ABSTRACT: Physicochemical properties (paste clarity, water-binding capacity, swelling power, solubility and thermal properties) and in vitro digestion of starches isolated from ten different Dioscorea plants were investigated. Moisture content ranged from 7.52% to 15.75% and protein content varied between 0.010% and 0.028%. All of the starches gave a typical C-type X-ray diffraction pattern except D.BY (CA-type pattern) and Dioscorea persimilis (B-type pattern) starches. The relative crystallinity of them varied from 12.02% to 51.68%. The starches displayed significant variability in thermal transition temperatures and susceptibility to in vitro digestion, and varied in rapidly digestible starch (RDS), slowly digestible starch (SDS), resistant starch (RS) contents, hydrolysis index (HI) and glycemic index (GI). The ten Dioscorea starch samples can be classified into two major clusters by hierarchical cluster analysis: one cluster with more D.PP, D.BY and D.RC, and another cluster with D. XM, D.M-1, D.M-2, D.TG, D.H, D.CJ and D.J.
    Food Hydrocolloids 08/2013; 32(2):432–439. · 3.49 Impact Factor
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    ABSTRACT: Yuanhu Zhitong prescription (YZP) is well known for its analgesic effect. However, its multiple bioactive components in vivo remain unclear. In this paper, a rapid resolution liquid chromatography/quadrupole time-of-flight (RRLC-ESI-Q/TOF) was employed to identify the bioactive components and partial metabolites after oral administration of YZP extracts. Meanwhile, a RRLC-ESI-Q/TOF method was established and validated for the simultaneous quantification of protopine, α-allocryptopine, tetrahydropalmatine, corydaline, tetrahyberberine and byakangelicin in rat plasma and applied for their pharmacokinetic research. The results showed that twenty-one bioactive components of YZP were absorbed into the blood circulation and seventeen components were detected in cerebrospinal fluid (CSF). Moreover, the kinetic profiles of six analytes were obtained and the results suggested that the six analytes peaked between 3.5 and 5.0h and Cmax ranged from 214.6 to 858.3. The works could provide key information for identification of bioactive constituents and understanding the metabolism as well as pharmacological actions for YZP.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 07/2013; 935C:1-9. · 2.78 Impact Factor
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    ABSTRACT: A rapid and simple solvent system screening method was developed for pH-zone-refining counter-current chromatography (CCC) separation, which was much easier to find a suitable solvent system than the traditional method. Using this method, an optimal solvent system composed of hexane-ethyl acetate-methanol-water (3:7:1:9, v/v) was selected for pH-zone-refining CCC separation of alkaloids from the stems of Gelsemium elegans, where 10mM triethylamine (TEA) was added to the upper organic stationary phase as a retainer and 10mM hydrochloric acid (HCl) to the aqueous mobile phase as an eluter. As a result, six oxindole alkaloids, 420mg 19-xo-gelsenicine, 456mg gelsemine, 723mg koumine, 379mg 11-methoxygelsemamide, 342mg gelsenicine and 318mg humantenine were successfully purified in one step from 4.5g crude extract with the purity of over 95%, respectively. The structures of the oxindole alkaloids were identified by ESI-MS, (1)H NMR and (13)C NMR.
    Journal of Chromatography A 07/2013; · 4.61 Impact Factor
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    ABSTRACT: The roots of Salvia miltiorrhiza Bge., named Danshen in traditional Chinese medicine, is widely used for the treatment of cardiovascular disorders, also for food auxiliary agent. Rosmarinic acid, salvianolic acid B, dihydrotanshinone, cryptotanshinone, and tanshinone IIA are all responsible for the pharmacological activity of Danshen, they are important quality evaluation index for Danshen. The quality evaluation of Danshen is mainly concentrated in difference of cultivated regions and years, but the difference in root systems themselves and the cause of the difference were overlook. The growth rings were employed to identified the roots ages in root systems. Then the contents of eight bioactive component in different aged, diameter and tissue of individual roots of the same root system were evaluated using HPLC coupled with an ultraviolet photodiode array detector. The root system of S. miltiorrhiza was a complex root systems composed by different aged and diameter roots, in which the contents of bioactive compounds vary greatly from the other. The lipophilic diterpenoid quinones mainly distributed in the bark and increased with the cultivated years. The hydrophilic depsides distributed in all tissues, and the contents are relatively high in xylem in 1-year-old roots. It was very important to identify the individual root age of S. miltiorrhiza root system cultivated for several years. In the same root system of S. miltiorrhiza roots with 0.4-cm diameter contain more bioactive compounds, which maybe cause by the uneven distribution of bioactive compounds. Microsc. Res. Tech., 2013. © 2013 Wiley Periodicals, Inc.
    Microscopy Research and Technique 07/2013; · 1.59 Impact Factor
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    ABSTRACT: Cytochrome P450 enzymes (CYPs) play major roles in generating highly functionalized terpenoids, but identifying the exact biotransformation step(s) catalyzed by plant CYP in terpenoid biosynthesis is extremely challenging. Tanshinones are abietane-type norditerpenoid naphthoquinones that are the main lipophilic bioactive components of the Chinese medicinal herb danshen (Salvia miltiorrhiza). Whereas the diterpene synthases responsible for the conversion of (E,E,E)-geranylgeranyl diphosphate into the abietane miltiradiene, a potential precursor to tanshinones, have been recently described, molecular characterization of further transformation of miltiradiene remains unavailable. Here we report stable-isotope labeling results that demonstrate the intermediacy of miltiradiene in tanshinone biosynthesis. We further use a next-generation sequencing approach to identify six candidate CYP genes being coregulated with the diterpene synthase genes in both the rhizome and danshen hairy roots, and demonstrate that one of these, CYP76AH1, catalyzes a unique four-electron oxidation cascade on miltiradiene to produce ferruginol both in vitro and in vivo. We then build upon the previous establishment of miltiradiene production in Saccharomyces cerevisiae, with incorporation of CYP76AH1 and phyto-CYP reductase genes leading to heterologous production of ferruginol at 10.5 mg/L. As ferruginol has been found in many plants including danshen, the results and the approaches that were described here provide a solid foundation to further elucidate the biosynthesis of tanshinones and related diterpenoids. Moreover, these results should facilitate the construction of microbial cell factories for the production of phytoterpenoids.
    Proceedings of the National Academy of Sciences 06/2013; · 9.74 Impact Factor
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    ABSTRACT: As an important concept in modern ecology, allometry is the differential growth of parts or metrics of an organism and refers to the numerical relationship between growth and allocation. Plant allometry has a close relationship with phenotypic plasticity. The application of allometric relation in plant research includes response to different habitats, reproductive strategy, intraspecific and interspecific competition, and so on. It could be useful to introduce the allometry into the practice of choosing suitable habitat, accumulation of medicinal parts biomass and chemical constituents, as well as scientific cultivation and management on Dao-di herbs.
    Scientia Sinica Vitae. 06/2013; 43(6):457-463.

Publication Stats

359 Citations
221.02 Total Impact Points

Institutions

  • 2011–2014
    • Chinese Academy of Medical Sciences
      Peping, Beijing, China
    • China Academy of Traditional Chinese Medicine
      Peping, Beijing, China
    • Nanjing University of Traditional Chinese Medicine
      Peping, Beijing, China
    • Clemson University
      Clemson, South Carolina, United States
    • 302 Military Hospital of China
      Peping, Beijing, China
  • 2010–2014
    • China Academy of Chinese Medical Sciences
      • Institute of Chinese Material Medica
      Peping, Beijing, China
  • 2013
    • Chinese Academy of Sciences
      • Dalian Institute of Chemical Physics
      Peping, Beijing, China
    • Capital Medical University
      • School of Traditional Chinese Medicine
      Beijing, Beijing Shi, China
    • Northeast Institute of Geography and Agroecology
      • Institute of Automation
      Beijing, Beijing Shi, China
    • Taishan Medical University
      Taishan, Jiangxi Sheng, China
  • 2010–2013
    • Shandong Academy of Sciences
      Chi-nan-shih, Shandong Sheng, China
    • Tianjin University of Traditional Chinese Medicine
      T’ien-ching-shih, Tianjin Shi, China
  • 2012
    • Yunnan Academy of Agricultural Sciences
      Yün-nan, Yunnan, China
    • Southwest Jiaotong University
      Hua-yang, Sichuan, China
    • Chongqing Municipal Academy of Chinese Materia Medica
      Ch’ung-ch’ing-shih, Chongqing Shi, China
    • Shandong University of Traditional Chinese Medicine
      Shan-tang, Jiangxi Sheng, China
  • 2009–2012
    • Tianjin University
      • School of Pharmaceutical Science and Technology
      Tianjin, Tianjin Shi, China
    • Hangzhou Normal University
      • College of Life and Environmental Sciences
      Hangzhou, Zhejiang Sheng, China
  • 2010–2011
    • Jinan University (Guangzhou, China)
      Shengcheng, Guangdong, China
  • 2009–2010
    • Beijing University of Chinese Medicine and Pharmacology
      • College of Chinese Materia Medica
      Peping, Beijing, China