Luqi Huang

China Academy of Chinese Medical Sciences, Peping, Beijing, China

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Publications (197)331.04 Total impact

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    ABSTRACT: Isopentenyl diphosphate isomerase (IPI) catalyzes the isomerization between the common terpene precursor substances isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) during the terpenoid biosynthesis process. In this study, tissue expression analysis revealed that the expression level of the Salvia miltiorrhiza IPI1 gene (SmIPI1) was higher in the leaves than in the roots and stems. Furthermore, color complementation and RNA interference methods were used to verify the function of the SmIPI1 gene from two aspects. A recombinant SmIPI1 plasmid was successfully constructed and transferred into engineered E. coli for validating the function of SmIPI1 through the color difference in comparison to the control group; the observed color difference indicated that SmIPI1 served in promoting the accumulation of lycopene. Transformant hairy root lines with RNA interference of SmIPI1 were successfully constructed mediated by Agrobacterium rhizogenes ACCC 10060. RNA interference hairy roots had a severe phenotype characterized by withering, deformity or even death. The mRNA expression level of SmIPI1 in the RSi3 root line was only 8.4% of that of the wild type. Furthermore the tanshinone content was too low to be detected in the RNA interference lines. These results suggest that SmIPI1 plays a critical role in terpenoid metabolic pathways. Addition of an exogenous SmIPI1 gene promoted metabolic flow toward the biosynthesis of carotenoids in E. coli, and SmIPI1 interference in S. miltiorrhiza hairy roots may cause interruption of the 2-C-methyl-D-erythritol-4-phosphate metabolic pathway.
    Molecules 11/2015; 20(11):20206-20218. DOI:10.3390/molecules201119689 · 2.42 Impact Factor
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    Chao Jiang · Yuan Yuan · Libing Liu · Jingyi Hou · Yan Jin · Luqi Huang ·
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    ABSTRACT: A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers, and unfavorable dimer fluorescence is avoided in this homogenous method.
    Scientific Reports 11/2015; 5:16260. DOI:10.1038/srep16260 · 5.58 Impact Factor

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    ABSTRACT: 1-Deoxy-d-xylulose-5-phosphate synthase (DXS) and 1-deoxy-d-xylulose-5-phosphate reductoisomerase (DXR) genes are the key enzyme genes of terpenoid biosynthesis but still unknown in Tripterygium wilfordii Hook. f. Here, three full-length cDNA encoding DXS1, DXS2 and DXR were cloned from suspension cells of T. wilfordii with ORF sizes of 2154 bp (TwDXS1, GenBank accession no.KM879187), 2148 bp (TwDXS2, GenBank accession no.KM879186), 1410 bp (TwDXR, GenBank accession no.KM879185). And, the TwDXS1, TwDXS2 and TwDXR were characterized by color complementation in lycopene accumulating strains of Escherichia coli, which indicated that they encoded functional proteins and promoted lycopene pathway flux. TwDXS1 and TwDXS2 are constitutively expressed in the roots, stems and leaves and the expression level showed an order of roots > stems > leaves. After the suspension cells were induced by methyl jasmonate, the mRNA expression level of TwDXS1, TwDXS2, and TwDXR increased, and triptophenolide was rapidly accumulated to 149.52 µg·g(-1), a 5.88-fold increase compared with the control. So the TwDXS1, TwDXS2, and TwDXR could be important genes involved in terpenoid biosynthesis in Tripterygium wilfordii Hook. f.
    International Journal of Molecular Sciences 10/2015; 16(10):25516-25535. DOI:10.3390/ijms161025516 · 2.86 Impact Factor
  • Gangping Hao · Xingyu Jiang · Lei Feng · Ru Tao · Yanling Li · Luqi Huang ·
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    ABSTRACT: The water-soluble phenolic acids of S. miltiorrhiza Bge. f. alba can protect against herbivores or pathogenic bacteria and have numerous pharmacological activities that are beneficial to human health. The main phenolic acids in S. miltiorrhiza, including rosmarinic acid and salvianolic acid B, are derived from the rosmarinic acid biosynthetic pathway, which has been partially characterized. However, little is known about the endogenous bHLH and MYB transcription factors that function in this pathway. In this paper, we describe the cloning and functional characterization of SmPAP1, a cDNA of the R2R3-MYB transcription factor isolated from S. miltiorrhiza Bge. f. alba. SmPAP1 contains an open reading frame of 645 bp in length and encodes a putative 214-amino acid protein. The deduced amino acid sequence of the N-terminal R2R3 repeat sequence of SmPAP1 shares high identity with other DNA-binding domains of plant MYB-type proteins. Expression studies have indicated that SmPAP1 is mainly expressed in leaves. The expression of SmPAP1 was induced by 0.1 mM methyl jasmonate, salicylic acid and abscisic acid in the hairy roots of S. miltiorrhiza Bge. f. alba. Yeast two-hybrid assays have demonstrated that SmPAP1 can interact with the bHLH factor SmMYC2 and heterologous AtMYC2, which are TFs known to induce anthocyanin synthesis in Arabidopsis. A transient co-expression experiment in tobacco leaves showed that SmPAP1 activated the promoters of two key rosmarinic acid biosynthetic pathway genes of S. miltiorrhiza, SmPAL1 (phenylalanine ammonia lyase) and SmC4H (cinnamic acid 4-hydroxylase). The overexpression of SmPAP1 induced the substantial accumulation of rosmarinic acid, salvianolic acid B, total phenolics and total flavonoids in the roots of transgenic S. miltiorrhiza Bge. f. alba. These data suggest that SmPAP1 is involved in the regulation of the phenolic acid biosynthetic pathway by interacting with bHLH TFs and activating genes encoding key enzymes in S. miltiorrhiza Bge. f. alba.
    Plant Cell Tissue and Organ Culture 09/2015; DOI:10.1007/s11240-015-0883-3 · 2.13 Impact Factor
  • Changlei Sun · Jia Li · Daijie Wang · Jin-Qian Yu · Xiao Wang · Luqi Huang ·
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    ABSTRACT: Litsea cubeba is characterized by the presence of aporphine alkaloids. But few recent reports about the preparative separation of alkaloids from L. cubeba are found. The traditional separation method is time consuming and solvent consuming and irreversible adsorption is inevitable. In this research, pH-zone-refining counter-current chromatography and high-speed counter-current chromatography are applied to separate the alkaloids from a chloroform extract of L. cubeba. The crude extract was fractionated using the solvent system: chloroform-methanol-water (4:3:3, v/v) with different concentrations of hydrochloric acid (retainer) in the aqueous stationary phase and triethylamine (eluter) in the organic mobile phase to determine the ideal conditions for screening for the aporphine alkaloids. Using 1.5 g of the chloroform extract, 68.1 mg of norisocorydine (93.5% purity), 215.5 mg of isoboldine (96.3% purity), 612.3 mg of the mixture of boldine and laurotetanine, 108.8 mg of reticuline (97.4% purity) and 92.6 mg of laurolitsine (97.6% purity) were obtained with the selected conditions where 60 mM of hydrochloric acid was added to the stationary phase and 10 mM of triethylamine was used in the mobile phase. The mixture of boldine and laurotetanine was further separated using high-speed counter-current chromatography with a two-phase solvent system composed of ethyl acetate-methanol-water (4:1:5, v/v). Two alkaloids, laurotetanine (285.7 mg) and boldine (112.3 mg), were obtained from 500 mg of the mixture, in a one-step separation, with the relative purity of 94.8% and 96.2%, respectively. The purities of the isolated alkaloids were determined using high performance liquid chromatography and the chemical structures were confirmed using electrospray ionization-mass spectrometry, proton nuclear magnetic resonance (1H-NMR) and carbon-13 (13C)-NMR.
    RSC Advances 08/2015; 5(92):75831-75837. DOI:10.1039/c5ra10564a · 3.84 Impact Factor
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    ABSTRACT: Rhizosphere and root-associated microbial communities are known to be related to soil-borne disease and plant health. In the present study, the microbial communities in rhizosphere soils and roots of both healthy and diseased Panax notoginseng were analyzed by high-throughput sequencing of 16S rRNA for bacteria and 18S rRNA internal transcribed spacer for fungi, to reveal the relationship of microbial community structure with plant health status. In total, 5593 bacterial operational taxonomic units (OTUs) and 963 fungal OTUs were identified in rhizosphere soils, while 1794 bacterial and 314 fungal OTUs were identified from root samples respectively. Principal coordinate analysis separated the microbial communities both in the rhizosphere soils and roots of diseased P. notoginseng from healthy plants. Compared to those of healthy P. notoginseng, microbial communities in rhizosphere soils and roots of diseased plants showed a decrease in alpha diversity. By contrast, bacterial community dissimilarity increased and fungal community dissimilarity decreased in rhizosphere soils of diseased plants, while both bacterial and fungal community dissimilarity in roots showed no significant difference between healthy and diseased plants. Redundancy analysis at the phylum level showed that mycorrhizal colonization and soil texture significantly affected microbial community composition in rhizosphere soils, whereas shoot nutrition status had a significant effect on microbial community composition in root samples. Our study provided strong evidence for the hypothesis that microbial diversity could potentially serve as an indicator for disease outbreak of medicinal plants, and supported the ecological significance of microbial communities in maintaining plant healthy and soil fertility.
    Antonie van Leeuwenhoek 08/2015; 108(5). DOI:10.1007/s10482-015-0560-x · 1.81 Impact Factor
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    ABSTRACT: Tripterygium wilfordii Hook.F. is one of the most valuable medicinal plants because it contains a large variety of active terpenoid compounds, including triptolide, celastrol and wilforlide. All of the pharmacologically active secondary metabolites are synthesized from the 2-C-methyl-D-erythritol 4-phosphateand mevalonate pathway in the isoprenoid biosynthetic system. The key step in this pathway is the isomerization of dimethylallyl diphosphate and isopentenyl diphosphate, which is catalyzed by isopentenyl-diphosphate-isomerase (IPP). In the present study, a full-length cDNA encoding IPI (designate as TwIPI, GenBank accession no.KT279355) was cloned from a suspension of cultured cells from T. wilfordii. The full-length cDNA of TwIPI was 1564 bp and encoded a polypeptide of 288 amino acids. The bioinformatics analysis showed that the deduced TwIPI sequence contained the TNTCCSHPL and WGEHELDY motif. The transcription level of the TwIPI in the suspension cells increased almost five-fold after treatment with methyl jasmonate as an elicitor. A functional color assay in Escherichia coli indicated that TwIPI could promote the accumulation of lycopene and encoded a functional protein. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Biotechnology and Applied Biochemistry 08/2015; DOI:10.1002/bab.1427 · 1.36 Impact Factor
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    ABSTRACT: Angelica sinensis (Apiaceae) is an endangered alpine herb that is widely used as a medicinal plant in traditional Chinese medicine (TCM). Wild populations of A. sinensis have become quite rare in China. Thus, population genetics studies of this species are urgently needed for its effective conservation and sustainable use. However, to date, no microsatellite loci have been isolated in A. sinensis. To address this issue, we isolated 18 polymorphic loci and genotyped 120 individuals collected from 6 populations. The number of alleles per locus ranged from 1.2 to 5.5, and the average was 2.4. The observed and expected heterozygosity per locus for a population varied, respectively, from 0.000 to 0.983 (averaged at 0.198) and from 0.066 to 0.661 (averaged at 0.333). Deviation from the HardyeWeinberg equilibrium (p < 0.01) was observed for 4 to 14 loci in various populations. These microsatellite markers were cross-amplified in 10 species affinis, and 7 loci were successfully amplified in all species. These microsatellite markers are useful for genetic studies, the conservation management of A. sinensis, and identification of A. sinensis.
    Biochemical Systematics and Ecology 07/2015; 61:488-492. DOI:10.1016/j.bse.2015.07.013 · 0.97 Impact Factor
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    Wei Zhang · Yuan Yuan · Shuo Yang · Jianjun Huang · Luqi Huang ·
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    ABSTRACT: DNA barcoding is a promising species identification method, but it has proved difficult to find a standardized DNA marker in plant. Although the ITS/ITS2 RNA transcript has been proposed as the core barcode for seed plants, it has been criticized for being too conserved in some species to provide enough information or too variable in some species to align it within the different taxa ranks. We selected 30 individuals, representing 16 species and four families, to explore whether ITS2 can successfully resolve species in terms of secondary structure. Secondary structure was predicted using Mfold software and sequence-structure was aligned by MARNA. RNAstat software transformed the secondary structures into 28 symbol code data for maximum parsimony (MP) analysis. The results showed that the ITS2 structures in our samples had a common four-helix folding type with some shared motifs. This conserved structure facilitated the alignment of ambiguous sequences from divergent families. The structure alignment yielded a MP tree, in which most topological relationships were congruent with the tree constructed using nucleotide sequence data. When the data was combined, we obtained a well-resolved and highly supported phylogeny, in which individuals of a same species were clustered together into a monophyletic group. As a result, the different species that are often referred to as the herb "Mu tong" were successfully identified using short fragments of 250 bp ITS2 sequences, together with their secondary structure. Thus our analysis strengthens the potential of ITS2 as a promising DNA barcode because it incorporates valuable secondary structure information that will help improve discrimination between species.
    PLoS ONE 07/2015; 10(7):e0131185. DOI:10.1371/journal.pone.0131185 · 3.23 Impact Factor
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    ABSTRACT: The medicinal plant Salvia miltiorrhiza produces various tanshinone diterpenoids that have pharmacological activities such as vasorelaxation, against ischemia-reperfusion injury, and antiarrhythmic effects. Their biosynthesis is initiated from the general diterpenoid precursor (E,E,E)-geranylgeranyl diphosphate by sequential reactions catalyzed by copalyl diphosphate synthase (CPS) and kaurene synthase-like (KSL) cyclases. Here is reported characterization of these enzymatic families from S. miltiorrhiza, which has led to the identification of novel pathways, including roles for separate CPSs in tanshinone production in roots versus aerial tissues (SmCPS1 and SmCPS2, respectively), as well as the novel production of ent-13-epi-manoyl oxide by SmCPS4 and SmKSL2 in floral sepals. The conserved SmCPS5 is involved in gibberellin plant hormone biosynthesis. Down-regulation of SmCPS1 by RNAi resulted in substantial reduction of tanshinones, and metabolomics analysis revealed 21 potential intermediates, indicating a complex network for tanshinone metabolism defined by certain key biosynthetic steps. Notably, the correlation between conservation pattern and stereochemical product outcome of the CPSs observed here, suggests a degree of correlation that, especially when combined with the identity of certain key residues, may be predictive. Accordingly, this study provides molecular insights into the evolutionary diversification of functional diterpenoids in plants. Copyright © 2015, Plant Physiology.
    Plant physiology 06/2015; 169(3). DOI:10.1104/pp.15.00695 · 6.84 Impact Factor
  • Linjie Qi · Jian Yang · Yuan Yuan · Luqi Huang · Ping Chen ·
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    ABSTRACT: MYB proteins are involved in many significant physiological and biochemical processes, including regulation of primary and secondary metabolism, flavonoid biosynthesis, response to various biotic and abiotic stresses, hormone synthesis and signal transduction. The functions of R2R3-MYB proteins in Scutellaria baicalensis Georgi under abiotic stress, however, has not been elucidated. To study the molecular mechanism by which MYB2 and MYB7 respond to abiotic stress in S. baicalensis, we analyzed the phenylpropanoid content, growth phenotype, antioxidant enzyme activity and flavonoid synthesis-associated gene expression in SbMYB2 or SbMYB7-overexpressing transgenic tobacco plants after treatment with NaCl, mannitol and abscisic acid (ABA). The transgenic tobacco showed a higher fresh weight than did the wild type (WT) tobacco. In contrast, antioxidant enzyme activity and flavonoid synthesis-related gene expression were markedly higher in WT tobacco after treatment with NaCl, mannitol and ABA, as compared to transgenic plants, This is likely because increased phenylpropanoid accumulation in transgenic tobacco plants played a central role in abiotic stress resistance. These results indicate that overexpression of SbMYB2 or SbMYB7 increased phenylpropanoid accumulation and enhanced NaCl, mannitol and ABA stresses tolerance in transgenic tobacco. Copyright © 2015 Elsevier Masson SAS. All rights reserved.
    Plant Physiology and Biochemistry 06/2015; 94:235-243. DOI:10.1016/j.plaphy.2015.06.007 · 2.76 Impact Factor
  • Fajie Li · Yuan Yuan · Hua Li · Zhilai Zhan · Liping Kang · Man Li · Bin Yang · Luqi Huang ·
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    ABSTRACT: Tetrabutylphosphonium hydroxide (TBPH) aqueous solution, a novel ionic liquid that could dissolve cellulose rapidly at ambient temperature (25 °C), was used for the first time to develop an extraction method for salidroside from Rhodiola crenulata, used as the model sample, with infrared-assisted extraction (IRAE) in this paper. IRAE-TBPH procedures were optimized using a series of single-factor experiments and under the optimal conditions, the IRAE-TBPH technique not only took a shorter time (from 1.0 h to 8 min) but also afforded a higher extraction rate of salidroside from the herbs (increased by 15.41-38.65%) compared with other extraction techniques, such as TBPH-based heat reflux extraction (HRE-TBPH), ultrasound-assisted extraction (UAE-TBPH) and conventional solvent (methanol, ethanol and pure water) based IRAE. The results indicated IRAE-TBPH to be a fast and efficient extraction technique. Furthermore, the mechanism of IRAE-TBPH was preliminarily studied by means of surface structures and chemical compositions of samples before and after different extraction techniques. On the basis of the destruction of herb surface microstructures, cellulose dissolving property of TBPH and high efficiency heating of infrared irradiation in IRAE-TBPH process, the IRAE-TBPH technique eventually got the maximum yield value. Therefore, TBPH solution as a novel, effective and alternative solvent with higher extraction efficiency in the IRAE of active compounds from medicinal plants showed a great promising prospect.
    RSC Advances 05/2015; 5(59). DOI:10.1039/C5RA07969A · 3.84 Impact Factor
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    ABSTRACT: Color variation in sea cucumber is one of the most crucial traits affecting price and taste in East Asian countries. However, the relationship and taxonomic status of the three color variants are still unclear. We used 14 samples that covered all three color variants and their geographic distributions, to construct the first phylogeny for the color variants based on the complete mitochondrial genome sequence and a number of tree-building methods (maximum parsimony (MP), maximum likelihood (ML), and Bayesian inference (BI)). The divergence times within color variants were estimated by the Bayesian molecular clock approach using the BEAST program. Our results showed that the color variants were not monophyletic in the well-resolved phylogenetic tree, which strongly refuted their separate species status. The molecular dating estimate revealed that the sea cucumber was a young group, which originated in the early Miocene period (22.03 mya) and rapidly diverged after the late Miocene period. It is interesting that individuals within each variant or geographic distribution were not always closely related and thus did not share a common origin. We propose that although they differ in body color, the three color morphs all belong to a single species of Apostichopus japonicus and the historical marine climate and the hydrographic complexity of the ocean currents could be responsible for their present distribution patterns.
    Mitochondrial DNA 05/2015; DOI:10.3109/19401736.2015.1022765 · 1.21 Impact Factor
  • Xiaohua Jin · Luqi Huang ·

    Taxon 05/2015; 64(2). DOI:10.12705/642.19 · 3.30 Impact Factor
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    ABSTRACT: Dragon's blood is a famous traditional Chinese medicine produced from source plants under bio- or abio-stress. Dracaena cochinchinensis (Lour.) S.C. Chen xylem (DX) is one of the most important sources of the medicine. In this work, a GC-MS method was developed for analysis of the n-hexane extracts of DX with resin (DXR) and without resin (DXW). The repeatability of the method was also investigated for a metabolite comparative study of the different xylems. About 80 components were detected, 26 of which were identified in both DXR and DXN. Three sesquiterpenes (τ-cadinol, τ-muurolon and α-cadinol) were first discovered in Dracaena cochinchinensis (Lour.) S.C. Chen. The chromatographs of the two plant materials were compared and differences of compounds were found. It showed that phytosterols showed a dramatic rise in content, and sesquiterpenes were found to be synthesized in DXR. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.
    Biomedical Chromatography 05/2015; 29(11). DOI:10.1002/bmc.3488 · 1.72 Impact Factor
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    ABSTRACT: Authentication is the first priority when evaluating the quality of Chinese herbal medicines, particularly highly toxic medicines. The most commonly used authentication methods are morphological identification and microscopic identification. Unfortunately, these methods could not effectively evaluate some herbs with complex interior structures, such as root of Aconitum species with a circular conical shape and an interior structure with successive changes. Defining the part that should be selected as the standard plays an essential role in accurate microscopic identification. In this study, we first present a visual 3D model of Aconitum carmichaeli Debx. constructed obtained from microscopic analysis of serial sections. Based on this model, we concluded that the point of largest root diameter should be used as the standard for comparison and identification. The interior structure at this point is reproducible and its shape and appearance can easily be used to distinguish among species. We also report details of the interior structures of parts not shown in the 3D model, such as stone cells and cortical thickness. To demonstrate the usefulness of the results from the 3D model, we have distinguished the microscopic structures, at their largest segments, of the other three Aconitum species used for local habitat species of Caowu. This work provides the basis for resolution of some debate regarding the microstructural differences among these species. Thus, we conclude that the 3D model composed of serial sections has enabled the selection of a standard cross-section that will enable the accurate identification of Aconitum species in Chinese medicine. Microsc. Res. Tech., 2015. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.
    Microscopy Research and Technique 03/2015; 78(5). DOI:10.1002/jemt.22491 · 1.15 Impact Factor
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    ABSTRACT: Ginseng, which is the root of Panax ginseng (Araliaceae), has been used in Oriental medicine as a stimulant and dietary supplement for more than 7,000 years. Older ginseng plants are substantially more medically potent, but ginseng age can be simulated using unscrupulous cultivation practices. Telomeres progressively shorten with each cell division until they reach a critical length, at which point cells enter replicative senescence. However, in some cells, telomerase maintains telomere length. In this study, to determine whether telomere length reflects ginseng age and which tissue is best for such an analysis, we examined telomerase activity in the main roots, leaves, stems, secondary roots and seeds of ginseng plants of known age. Telomere length in the main root (approximately 1 cm below the rhizome) was found to be the best indicator of age. Telomeric terminal restriction fragment (TRF) lengths, which are indicators of telomere length, were determined for the main roots of plants of different ages through Southern hybridization analysis. Telomere length was shown to be positively correlated with plant age, and a simple mathematical model was formulated to describe the relationship between telomere length and age for P. ginseng.
    Scientific Reports 01/2015; 5:7985. DOI:10.1038/srep07985 · 5.58 Impact Factor
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    ABSTRACT: DNA barcoding has been proposed to be one of the most promising tools for accurate and rapid identification of taxa. However, few publications have evaluated the efficiency of DNA barcoding for the large genera of flowering plants. Dendrobium, one of the largest genera of flowering plants, contains many species that are important in horticulture, medicine and biodiversity conservation. Besides, Dendrobium is a notoriously difficult group to identify. DNA barcoding was expected to be a supplementary means for species identification, conservation and future studies in Dendrobium. We assessed the power of 11 candidate barcodes on the basis of 1,698 accessions of 184 Dendrobium species obtained primarily from mainland Asia. Our results indicated that five single barcodes, i.e., ITS, ITS2, matK, rbcL and trnH-psbA, can be easily amplified and sequenced with the currently established primers. Four barcodes, ITS, ITS2, ITS+matK, and ITS2+matK, have distinct barcoding gaps. ITS+matK was the optimal barcode based on all evaluation methods. Furthermore, the efficiency of ITS+matK was verified in four other large genera including Ficus, Lysimachia, Paphiopedilum, and Pedicularis in this study. Therefore, we tentatively recommend the combination of ITS+matK as a core DNA barcode for large flowering plant genera.
    PLoS ONE 01/2015; 10(1):e0115168. DOI:10.1371/journal.pone.0115168 · 3.23 Impact Factor
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    ABSTRACT: Deer antler is a precious animal-sourced traditional Chinese medicine. We aimed to rapidly assess the quality of deer antler slices by electronic nose so that we can ensure medical safety. In this study, response intensity of the electronic nose was favorably optimized, and samples were well assessed by using an electronic nose based on LDA model. The results obtained herein suggested that electronic nose could be an effective method to rapidly as- sess the quality of deer antler slices, and could also be an important tool for categorization of complex aroma mixtures for the control of quality of drugs or food.
    Revista Brasileira de Farmacognosia 12/2014; 22(6). DOI:10.1016/j.bjp.2014.10.011 · 0.83 Impact Factor

Publication Stats

1k Citations
331.04 Total Impact Points


  • 2009-2015
    • China Academy of Chinese Medical Sciences
      • Institute of Chinese Material Medica
      Peping, Beijing, China
  • 2010-2014
    • Beijing University of Chinese Medicine and Pharmacology
      • School of Chinese Materia Medica
      Peping, Beijing, China
  • 2009-2014
    • Chinese Academy of Medical Sciences
      Peping, Beijing, China
  • 2012
    • Yunnan Academy of Agricultural Sciences
      Yün-nan, Yunnan, China
    • Southwest Jiaotong University
      Hua-yang, Sichuan, China
    • Shandong University of Traditional Chinese Medicine
      Shan-tang, Jiangxi Sheng, China
    • Chongqing Municipal Academy of Chinese Materia Medica
      Ch’ung-ch’ing-shih, Chongqing Shi, China
  • 2010-2012
    • Shandong Academy of Sciences
      Chi-nan-shih, Shandong Sheng, China
  • 2011
    • Nanjing University of Traditional Chinese Medicine
      Peping, Beijing, China
    • China Academy of Traditional Chinese Medicine
      Peping, Beijing, China
    • 302 Military Hospital of China
      Peping, Beijing, China
    • Jinan University (Guangzhou, China)
      Shengcheng, Guangdong, China
    • University of Padova
      • Department of Biology
      Padua, Veneto, Italy