Publications (46)34.02 Total impact
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Article: [Preparation, epitope analysis and clinical application of monoclonal antibodies against heart-type fatty acid binding protein].
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ABSTRACT: Objective To prepare and characterize the monoclonal antibody (mAb) against recombinant human heart-type fatty acid binding protein (H-FABP) and apply it to the clinical analysis. Methods BALB/c mice were immunized with recombinant H-FABP (rH-FABP) from prokaryotic expression or synthesized peptide fragment. After common fusion and screening, the subtypes, titer and affinity of mAbs were detected respectively. After purification, the specificity of mAbs was tested by indirect ELISA and Western blotting. ELISA system was established by pair mapping and applied in the detection of clinical samples. Two epitope peptides were designed by bioinformatics and used to detect the epitope of 1-F10 through Western blotting. Results Four different hybridoma clones secreting anti-H-FABP antibodies were developed, with high titres of 1:51 200-1:1024 000. Immunoglobulin types of these mAbs were found to be IgG2a or IgG2b, respectively. The affinity of the mAb 1-F10 even reached 9.02×10(9); mol/L. ELISA and Western blotting showed that these mAbs could identify H-FABP specifically. In addition, the pair mapping of monoclonal antibodies 3-H5-1-F10 could recognize H-FABP in human serum samples. Furthermore, the specific target recognized by 1-F10 mAb was located within amino acid 86-133 of H-FABP. Conclusion Four highly specific mAbs against H-FABP were successfully obtained and the established ELISA system could be used to detect the H-FABP in clinical serum samples, and the epitope of 1-F10 mAb was also verified.Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 06/2013; 29(6):620-4. -
Article: A monoclonal antibody specific to the non-epitope region of hepatitis B virus preS1 contributes to more effective HBV detection.
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ABSTRACT: OBJECTIVES: The hepatitis B virus (HBV) preS1 protein is divided into an epitope region and a non-epitope region based on the respective antigenicities of these regions. Most of the antibodies that are currently used to detect the large surface protein of HBV (HBV LHB) are specific to the epitope region of preS1, which may contribute to the false negative results of HBV LHB detection assays. Here, we established a mouse monoclonal antibody (mAb) that could improve the efficiency of HBV LHB detection. DESIGN AND METHODS: The HBV preS1 protein was expressed in E. coli and used to screen hybridoma clones. HBV preS1-specific mAb was produced by immunizing mice with a chemically synthesized peptide antigen derived from the non-epitope region of HBV preS1. The mAb was characterized by ELISA, Western blot, and immunocytochemistry and was subsequently used in serum sample tests. RESULTS: Based on in silico B cell epitope predictions, the HBV preS1 aa 91-117 peptide was synthesized as an antigen. Recombinant HBV preS1 was expressed in E. coli and identified by SDS-PAGE. The mAb D8 (IgG2b) recognized the recombinant preS1 protein in both ELISA and Western blot assays and also recognized the preS1 protein expressed in plasmid-transfected HepG2.2.15 cells by immunocytochemistry. Furthermore, the D8 mAb, which is specific for the non-epitope region of preS1, contributed to the improved sensitivity and specificity of HBV detection. CONCLUSIONS: We established a mAb that is specific to the non-epitope region of HBV preS1 and improved the detection of HBV LHB in an ELISA assay. This mAb could help increase the accuracy of the clinical measurement of preS1.Clinical biochemistry 04/2013; · 2.02 Impact Factor -
Article: Interferon regulatory factor 4 binding protein is a novel p53 target gene and suppresses cisplatin-induced apoptosis of breast cancer cells.
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ABSTRACT: Our previous work demonstrated that ectopic expression of interferon regulatory factor 4 binding protein (IBP) was correlated with the malignant behaviour of human breast cancer cells. The mechanisms controlling differential expression of IBP in breast cancer still remain unknown. To investigate the mechanism of IBP dysregulation in breast cancer, we identified IBP was a novel p53 target gene. IBP expression was negatively regulated by wild-type p53 and was p53 dependently suppressed by DNA damage agent cisplatin. Furthermore, high levels of IBP were found to decrease cisplatin-induced growth suppression and apoptotic cell death, which was associated with decreased p53 activity and imbalanced Bcl-2 family member expression. IBP is a novel p53 target gene which suppresses cisplatin-mediated apoptosis of breast cancer cells via negative feedback regulation of the p53 signalling pathway, suggesting IBP may serve as a target for pharmacologic intervention of breast cancer resistant to cisplatin therapy.Molecular Cancer 08/2012; 11:54. · 3.99 Impact Factor -
Article: Evolution of a coastal upwelling event during summer 2004 in the southern Taiwan Strait
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ABSTRACT: A coastal upwelling event in the southern Taiwan Strait (STWS) was investigated using intensive cruise surveys (four repeated transects in a month) and satellite data in July and early August 2004. The extensive upwelling-associated surface cold water was first observed in early July (∼2.0×104 km2) along the STWS coast. Then, the cold surface water reduced in size by ∼50% with decreased chlorophyll concentrations after 15 days, indicating the weakening of the upwelling event. At the end of July, the cold surface water disappeared. The temporal variations of the surface cold water and the 3-D hydrography around Dongshan Island are thought to be mainly attributed to the weakened upwelling-favorable southwestern wind, the asymmetric spatial structure of the wind field and the intrusion of warm water from the northern South China Sea. Key wordscoastal upwelling–evolution–SST–ocean color–remote sensing–Taiwan StraitActa Oceanologica Sinica -English Edition- 04/2012; 30(1):1-6. · 0.49 Impact Factor -
Article: Physical environments of the Caribbean Sea
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ABSTRACT: The Caribbean Sea encompasses a vast range of physical environmental conditions that have a profound influence on the organisms that live there. Here we utilize a range of satellite and in situ products to undertake a region-wide categorization of the physical environments of the Caribbean Sea (PECS). The classification approach is hierarchical and focuses on physical constraints that drive many aspects of coastal ecology, including species distributions, ecosystem function, and disturbance. The first level represents physicochemical properties including metrics of satellite sea surface temperature, water clarity, and in situ salinity. The second level considers mechanical disturbance and includes both chronic disturbance from wind-driven wave exposure and acute disturbance from hurricanes. The maps have a spatial resolution of 1 km(2). An unsupervised neural network classification produced 16 physicochemical provinces that can be categorized into six broad groups: (1) low water clarity and low salinity and average temperatures; (2) low water clarity but average salinity and temperature, broadly distributed in the basin; (3) low salinity but average water clarity and temperature; (4) upwelling; (5) high latitude; and (6) offshore waters of the inner Caribbean. Additional mechanical disturbance layers impose additional pattern that operates over different spatial scales. Because physical environments underpin so much of coastal ecosystem structure and function, we anticipate that the PECS classification, which will be freely distributed as geographic information system layers, will facilitate comparative analyses and inform the stratification of studies across environmental provinces in the Caribbean basin.Limnology and Oceanography. 01/2012; 57(4):1233-1244. -
Article: Chlorophyll variability in the northeastern Gulf of Mexico
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ABSTRACT: Changes in chlorophyll concentration distribution in surface waters of the northeastern Gulf of Mexico (NEGOM) were examined using satellite and in situ data collected between November 1997 and August 2000. The patterns of chlorophyll distribution derived from in situ data consistently matched the satellite observations, even though the satellite-derived concentrations in coastal and offshore waters influenced by rivers were overestimated by the standard satellite data processing algorithms. River discharge and wind-driven upwelling were the major factors influencing surface chlorophyll-a variability for inshore regions. High in situ chlorophyll-a concentrations (≥1 mg m−3) occurred inshore and particularly near major river mouths during the summer seasons of 1998, 1999 and 2000. Plumes of Mississippi River water extended offshore to the southeast of the delta over distances >500 km from the river delta for maximum periods of 14 weeks between May and September every year and could reach the Florida Keys in certain years. The offshore transport of the plume was initiated by eastward or southeastward winds and then by separate anticyclonic eddies located southeast of the Mississippi delta and nearby shelf every year. Chlorophyll concentrations during the winter to spring transition in 1998 off Escambia, Choctawhatchee, Apalachicola and Suwannee Rivers and off Tampa Bay were up to 4 times higher than during the same periods in 1999 and 2000. This was related to higher freshwater discharge during the 1997–1998 winter–spring transition, coinciding with an El Niño–Southern Oscillation event, and to the unusually strong upwelling observed along the coast in spring 1998.International Journal of Remote Sensing 12/2011; 32(23):8373-8391. · 1.12 Impact Factor -
Article: A novel microfluidic immunoassay system based on electrochemical immunosensors: an application for the detection of NT-proBNP in whole blood.
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ABSTRACT: Electrochemical immunosensors have attracted great interest in the search for a selective, simple and reliable system for molecular recognition. Presently, electrochemical immunosensors have been widely studied for biomedical molecular's detection, but the regeneration of these immunosensors has restricted their wide application. To prepare a regeneration-free immunosensor, which may be more suitable for clinical determination, a repeatable immunoassay system was developed based on an electrochemical immunosensor with magnetic nanoparticles, biotin-avidin system (BAS) and Fab antibodies for the heart failure markers aminoterminal pro-brain natriuretic peptides (NT-proBNP). At the same time, a microfluidic system was combined into the proposed system, which enabled continuous determination. Using NT-proBNP as a model system, the proposed immunosensor exhibited rapid and sensitive amperometric response to NT-proBNP with good selectivity, stability, and a wide linear range (0.005-1.67 ng/mL and 1.67-4 ng/mL with a detection limit of 0.003 ng/mL under optimal conditions). Importantly, the proposed immunosensor was also suitable for the detection of other proteins and provided new opportunities for disease diagnosis.Biosensors & bioelectronics 11/2011; 31(1):480-5. · 5.43 Impact Factor -
Article: Elevation of plasma soluble CD26 levels during pregnancy.
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ABSTRACT: CD26 is a type II transmembrane protein with dipeptidyl peptidase IV (DPPIV) activity expressed on a variety of cell types. Recent studies have indicated that CD26 or enzymatic activity levels were previously associated with immune-mediated disorders. As immunoregulation is very important for a successful pregnancy, we hypothesize that CD26 may play an important role during pregnancy, and herein, we sought to determine the association between circulating levels of soluble CD26 (sCD26) and pregnancy outcome. In this study, a stable hybridoma cell line 1F1 was produced by fusion of murine splenocytes and myeloma cells. An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed for the detection of the maternal plasma sCD26. We measured the plasma levels of sCD26 in 80 normal pregnant women and 45 non-pregnant women. Our results indicated that the plasma level of sCD26 was significantly higher in the pregnant group (P < 0.001) than that in the non-pregnant group. These findings hinted that CD26 may play a role in successful pregnancy and it is not an absolute surrogate marker for the Th1-type immunity as the dominant Th2-type immunity during pregnancy.Journal of Obstetrics and Gynaecology Research 09/2011; 38(1):272-9. · 0.94 Impact Factor -
Article: Characterization, epitope identification and mechanisms of the anti-septic capacity of monoclonal antibodies against macrophage migration inhibitory factor.
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ABSTRACT: Sepsis is characterized by uncontrolled inflammatory responses. Macrophage migration inhibitory factor (MIF) has been shown to play an important role in the progression of sepsis thus is a potential therapeutic target. The aim of this study is to produce IgG anti-MIF monoclonal antibodies (mAbs) with anti-septic abilities in vivo and to determine mechanisms of their function. We generated 8 IgG anti-MIF mAbs with high specificity and 3 of them showed potent protective abilities in murine lethal peritonitis induced by cecal ligation and puncture (CLP). One anti-MIF mAb, F11, showed 100% protection within 72 h after sepsis induction and 72% mice treated with this mAb survived up to 84 h with reduced lung and kidney pathology. F11 treatment also reduced tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels in septic mice. We further found that all 8 anti-MIF mAbs recognized the same epitope located in the amino acid residue 1-20 region of the N terminus of the MIF protein. Three of the mAbs, F11 in particular, inhibited tautomerase activity in association with their protective effect on CLP mice. Thus, we have produced anti-MIF mAbs that protected mice from CLP-induced sepsis by recognizing the same epitope domains in MIF. These mAbs are promising candidates for further development of therapeutics against inflammatory diseases.International immunopharmacology 05/2011; 11(9):1333-40. · 2.21 Impact Factor -
Article: Application of a Fab fragment of monoclonal antibody specific to N-terminal pro-brain natriuretic peptide for the detection based on regeneration-free electrochemical immunosensor.
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ABSTRACT: N-Terminal pro-brain natriuretic peptide (NT-proBNP) is an important marker for heart failure that reflects ventricular volume expansion, ventricular overload and the degree of cardiac injury. To establish a sensitive and simple detection method for it in serum, a regeneration-free immunosensor with novel Fab fragment monoclonal antibodies was prepared. The sensor detected NT-proBNP from 0.04 to 2.5 ng/ml with a limit of 0.03 ng/ml. The immunosensor is therefore a simple, cost-effective method to detect NT-proBNP and the proposed immunoassay system would enable other proteins to be detected and open new opportunities for protein diagnostics.Biotechnology Letters 03/2011; 33(8):1539-43. · 1.68 Impact Factor -
Article: Tracking the Deepwater Horizon oil spill: A modeling perspective
Eos Transactions American Geophysical Union 01/2011; 92(6):45-46. -
Article: Overexpression of the Interferon regulatory factor 4-binding protein in human colorectal cancer and its clinical significance.
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ABSTRACT: IFN regulatory factor 4-binding protein (IBP) is a novel type of activator of Rho GTPases. It has been linked with differentiation and apoptosis of lymphocytes, but its function in oncogenesis remains unclear. Here we studied the expression of endogenous IBP in four human colorectal cancer cell lines, normal, adenoma and tumor colorectal tissues. Molecular (Western blot and RT-PCR), and confocal analyses were used to investigate IBP expression in human colorectal cancer cell lines. Matched normal and tumor tissue sections of 63 patients and 15 adenoma tissue sections were analyzed for IBP expression by immunohistochemistry (IHC). IBP was ubiquitely expressed in human colorectal cancer cell lines. The expression of IBP can be detected at both the mRNA and protein level in SW480, SW620 and HT29 cells. Clinically, IBP were elevated in human colorectal cancer specimens in comparison to normal colorectal tissues. Substantial high expression of IBP was observed in colorectal cancer tissues (67%), whereas corresponding normal tissues and 15 adenoma tissues showed consistently absent immunoreactivity of IBP. Moreover, IBP expression is correlated with the differentiation level of colorectal cancer cells (p<0.05) and clinical stage of patients (p<0.01). Our data show, for the first time, a dysregulated expression of IBP in human colorectal cancer, offering new perspectives for its role in cancer development and progression. IBP may be a novel tumor marker and a therapeutic target for colorectal cancer.Cancer epidemiology. 09/2009; 33(2):130-6. -
Article: A novel, label-free immunosensor for the detection of alpha-fetoprotein using functionalised gold nanoparticles.
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ABSTRACT: Novel immunoassay methods based on electrochemical sensors have been developed, but most of these immunosensors are unsuitable for clinical detection because their preparation requires complicated chemical procedures and because their detection sensitivity is restricted. In order to develop a highly sensitive, label-free amperometric sensor for immunoassays, we synthesised novel, functionalized gold nanoparticles (SV-GNP) by covalently capping the surface of gold nanoparticles (GNP) with 1,1'-bis-(2-mercapto)-4,4'-bipyridinium dibromide, a kind of sulfhyrdryl viologen (SV). We fabricated an immunosensor in a multi-step fashion, by first coating the SV-GNP onto a glassy carbon electrode surface; the resulting electrode core could then adsorb a suitable antibody in a second step to afford the desired immunosensor. alpha-fetoprotein (AFP) was used as a model analyte in this work. The anti-AFP/SV-GNP-modified electrode was sensitive to AFP with a linear relationship between 1.25 and 200 ng/mL and a correlation coefficient of 0.9983; the detection limit at a signal to noise ratio of 3 was 0.23 ng/mL under optimal conditions. In addition, the proposed immunosensor exhibited good sensitivity, selectivity, stability and long-term maintenance of bioactivity. The described immunosensor preparation and immunoassay methods offer promise for label-free, simple, and cost-effective analysis of biological samples.Clinical biochemistry 08/2009; 42(15):1524-30. · 2.02 Impact Factor -
Article: c-Rel is a transcriptional repressor of EPHB2 in colorectal cancer.
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ABSTRACT: The receptor tyrosine kinase EPHB2 has recently been identified as a TCF4 transcriptional target that controls the intestinal epithelial architecture through repulsive interactions with Ephrin-B ligands. Many reports have demonstrated that most human colorectal cancers lose EPHB2 expression despite constitutive Wnt activation. Therefore, we investigated the mechanisms that cause EPHB2 down-regulation in colorectal cancer. In this study, we demonstrate that DNA hypermethylation was not responsible for the frequent loss of EPHB2 expression in colorectal cancer. Cloning and functional characterization of the EPHB2 gene 5'-flanking region revealed a potential negative regulatory element in the distal regulatory region. In vitro electrophoretic gel mobility shift and in vivo chromatin immunoprecipitation assays demonstrated that c-Rel directly binds to the putative element. Inhibiting c-Rel activity or knocking down c-Rel expression by RNA interference in colon cancer cells was sufficient to induce EPHB2 expression. Furthermore, transient transfection assays demonstrated that c-Rel over-expression repressed endogenous EPHB2 expression in colon cancer cells. We demonstrate for the first time that c-Rel acts as a transcriptional repressor of EPHB2 and plays an active role in EPHB2 down-regulation in colorectal cancers.The Journal of Pathology 07/2009; 219(1):103-13. · 6.32 Impact Factor -
Article: The ectopic expression of IFN regulatory factor 4-binding protein is correlated with the malignant behavior of human breast cancer cells.
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ABSTRACT: Many proteins that are aberrantly expressed in malignant tumors play important roles in promoting tumorigenesis, metastasis and immune escape. IFN regulatory factor 4-binding protein (IBP), which is a novel PH-DH-like protein related to SWAP-70, and functions as an upstream activator of Rho GTPases. It is widely expressed in cells of the immune system and is involved in coupling activated cell receptors to downstream signaling events that mediate cell proliferation, differentiation and polarization. Although IBP was detected in human chondrosarcoma, its function in tumor cells remains unknown. In this study, newly generated monoclonal anti-IBP antibodies were employed and they detected higher level expression of IBP in some human invasive breast carcinoma tissues and in two breast cancer cell lines that form highly invasive tumors in nude mice. In contrast, the levels of IBP mRNA and protein were low or undetectable in normal human breast tissues, benign breast lesions or low-tumorigenic breast cancer cell lines. Over-expression of wild-type IBP in an IBP-negative breast cancer cell line markedly increased its proliferation and invasiveness in vitro. Conversely, RNA interference-mediated knockdown of IBP expression in an IBP-positive breast cancer cell line significantly reduced cell growth and invasiveness. Our results indicate that IBP is expressed in more highly invasive human breast cancer cells, such as MCF-7 and MDA-MB-231, with lower expression in normal breast tissue, benign tumors and less aggressive breast cancer cells, such as SKBR3 and MDA-MB-453. Thus, expression of IBP is correlated with the degree of malignant breast tumors. Nevertheless, it should be pointed our that further study with more tumor types is required to fully elucidate the role of IBP in tumorigenesis and the potential of IBP as a marker for more highly malignant tumors.International immunopharmacology 05/2009; 9(7-8):1002-9. · 2.21 Impact Factor -
Chapter: Multi-Scale Remote Sensing of Coral Reefs
09/2008: pages 297-315; -
Chapter: The Color of the Coastal Ocean and Applications in the Solution of Research and Management Problems
09/2008: pages 101-127; -
Article: Development of a novel method to measure macrophage migration inhibitory factor (MIF) in sera of patients with rheumatoid arthritis by combined electrochemical immunosensor.
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ABSTRACT: Macrophage migration inhibitory factor (MIF) has a multitude of biological activity and is associated with a number of inflammatory and immune diseases, including rheumatoid arthritis (RA). The increased serum levels of MIF in patients not only suggest this protein as a marker for disease progression, but also as a potential therapeutic target. The aim of this study is to develop a novel electrochemical method to more precisely and conveniently measure MIF in patient sera. An IgM murine monoclonal antibody (mAb) against human MIF was prepared and used in the electrochemical immunosensor, with modified gold electrode coated with compounds of gold nanoparticles, titanium dioxide nanoparticles and thionine (NGP-NTiP-Thi) followed by adsorption of anti-MIF antibodies with IgM or IgG1 isotype. The IgM immunosensor recognized MIF in a linear relationship in the range of 0.03 and 230 ng/mL with the lower limit (S/N=3) of 0.02 ng/mL. The measurement showed considerable levels of sensitivity, selectivity, stability and long-term maintenance of bioactivity, as shown by testing with serum MIF in RA patients as compared to healthy donors. The performance of the IgM immunosensor was also superior to IgG1 sensor. Thus, we have developed a novel measurement approach for serum MIF, which may have great potential in the clinic for monitoring the course of diseases associated with increased MIF.International Immunopharmacology 07/2008; 8(6):859-65. · 2.38 Impact Factor -
Article: Mississippi River water in the Florida Straits and in the Gulf Stream off Georgia in summer 2004
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ABSTRACT: 1] A coherent plume from the Mississippi River was detected in MODIS imagery in July – October 2004 extending from the eastern Gulf of Mexico into the Florida Straits (FS), and reaching the Gulf Stream (GS) off Georgia. The plume was sampled from ships in the FS and the GS. In early August, the plume was about 10– 20 km wide in the western FS and about 50 km wide off Georgia. The FS ship survey (16 – 26 August) documented a 50 km, 10 – 20 m deep band with anomalies in surface salinity (À0.8), temperature (0.5°C), and surface chlorophyll concentration (2Â) relative to FS waters outside the plume. Nutrient concentrations were only slightly higher in the FS plume and there was no apparent increase in colored dissolved organic matter. We estimate that the plume carried some 23% ($2778 m 3 s À1) of the Mississippi River discharge between July and September 2004 into the GS.Geophys. Res. Lett. 01/2005; 32. -
Article: Comparison of ship and satellite bio-optical measurements on the continental margin of the NE Gulf of Mexico
International Journal of Remote Sensing 01/2003; 24(13):2597-2612. · 1.12 Impact Factor
Top co-authors
Top Journals
Institutions
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2008–2012
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The Third Military Medical University
Chongqing, Chongqing Shi, China
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2003–2012
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University of South Florida St. Petersburg
Saint Petersburg, FL, USA
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2009
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Southwest University in Chongqing
Chongqing, Chongqing Shi, China
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