Paride Abliz

Publications (12)25.58 Total impact

• Article: Molecular typing of Trichophyton tonsurans by PCR-RFLP of the ribosomal DNA nontranscribed spacer region.

  Paride Abliz, Kayoko Takizawa, Kazuko Nishimura, Kazutaka Fukushima, Cristina Maria de Souza Motta, Oliane Maria Magallâos, Shuwen Deng, Liyan Xi, Valerio Vidotto
  Journal of Dermatological Science 12/2004; 36(2):125-7. · 3.72 Impact Factor
• Article: Identification of pathogenic dematiaceous fungi and related taxa based on large subunit ribosomal DNA D1/D2 domain sequence analysis.

  Paride Abliz, Kazutaka Fukushima, Kayoko Takizawa, Kazuko Nishimura
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  ABSTRACT: The nucleotide sequences of the D1/D2 domains of large subunit (26S) ribosomal DNA for 76 strains of 46 species of pathogenic dematiaceous fungi and related taxa were determined. Intra-species sequence diversity of medically important dematiaceous fungi including Phialophora verrucosa, Fonsecaea pedrosoi, Fonsecaea compacta, Cladophialophora carrionii, Cladophialophora bantiana, Exophiala dermatitidis, Exophiala jeanselmei, Exophiala spinifera, Exophiala moniliae, and Hortaea werneckii were extremely small; as few as 0 changes were detected in C. bantiana, Fonsecaea and Exophiala species, 1 bp in C. carrionii and H. werneckii, and 2 bp in P. verrucosa. Inter-species nucleotide diversity between most species was higher. These data suggested that the D1/D2 domain is sufficiently variable for identification of pathogenic dematiaceous fungi and relevant species. The phylogenetic trees constructed from the sequence data revealed that most human pathogenic species formed a single cluster and that Cladosporium and Phialophora species were distributed polyphyletically into several clusters.
  FEMS Immunology & Medical Microbiology 02/2004; 40(1):41-9. · 2.44 Impact Factor
• Source

  Available from: ncbi.nlm.nih.gov

  Article: Specific oligonucleotide primers for identification of Cladophialophora carrionii, a causative agent of chromoblastomycosis.

  Paride Abliz, Kazutaka Fukushima, Kayoko Takizawa, Kazuko Nishimura
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  ABSTRACT: Cladophialophora carrionii is one of the relatively common causative agents of chromoblastomycosis. We have developed the specific oligonucleotide primer set based on the internal transcribed spacer regions of ribosomal DNA for the rapid identification of this pathogen. PCR with this primer set amplified a 362-bp amplicon from C. carrionii strains. From other relevant dematiaceous species, including medically important dematiaceous fungi, such as Fonsecaea pedrosoi, Phialophora verrucosa, and Exophiala dermatitidis, and eight species of medically important yeasts, such as Candida albicans and Cryptococcus neoformans var. neoformans, the primer set did not produce any amplicon. PCR with this primer set may be a useful tool for the identification of C. carrionii.
  Journal of Clinical Microbiology 02/2004; 42(1):404-7. · 4.15 Impact Factor
• Article: Specific oligonucleotide primers for identification of Hortaea werneckii, a causative agent of tinea nigra.

  Paride Abliz, Kazutaka Fukushima, Kayoko Takizawa, Makato Miyaji, Kazuko Nishimura
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  ABSTRACT: Hortaea werneckii, a black yeast-like hyphomycete that is widely distributed in tropical and subtropical environments, can cause superficial mycotic infection in humans. This fungus was recently isolated from superficial infectious lesions of a guinea pig in Japan. An oligonucleotide primer set specific for Hortaea werneckii was designed on the basis of the internal transcribed spacer regions of the ribosomal DNA (rDNA). Polymerase chain reaction (PCR) with this primer set yielded a 306 bp PCR amplicon from only H. werneckii. This primer set did not amplify DNAs of 42 other related dematiaceous species, including the medically important dematiaceous fungi Cladophialophora carrionii, Fonsecaea pedrosoi, Phialophora verrucosa, and Exophiala dermatitidis, and eight species of medically important yeasts, including Candida (C.) albicans, C. dublinensis, C. glabrata, C. parapsilosis, C. tropicalis, Cryptococcus neoformans var. neoformans, Malassezia furfur, and Trichosporon asahii var. asahii. PCR with this primer set may be a useful technique for rapid identification of H. werneckii.
  Diagnostic Microbiology and Infectious Disease 07/2003; 46(2):89-93. · 2.53 Impact Factor
• Source

  Available from: ncbi.nlm.nih.gov

  Article: Rapid identification of the genus fonsecaea by PCR with specific oligonucleotide primers.

  Paride Abliz, Kazutaka Fukushima, Kayoko Takizawa, Norikazu Nieda, Makoto Miyaji, Kazuko Nishimura
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  ABSTRACT: An oligonucleotide primer set based on internal transcribed spacer regions of ribosomal DNA for PCR which gives the amplicon for only the DNA from Fonsecaea species was designed. This set yielded an amplicon with 333 bp for all strains of Fonsecaea pedrosoi and Fonsecaea compacta examined but no amplicons for related dematiaceous fungi and pathogenic yeasts. PCR using this primer set was considered to be a useful method for the rapid identification of the genus FONSECAEA:
  Journal of Clinical Microbiology 03/2003; 41(2):873-6. · 4.15 Impact Factor
• Source

  Available from: Kaoru Okada

  Article: Two new species of Neosartorya from Amazonian soil, Brazil

  Yoshikazu Horie, Paride Abliz, Kazutaka Fukushima, Kaoru Okada, G.M. Campos Takaki
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  ABSTRACT: Neosartorya indohii and N. tsurutae, two new Neosartorya species isolated from tropical rainforest soil in the Amazonian area, Brazil, are described and illustrated. Neosartorya indohii is characterized by its spreading growth on Czapeks and malt extract agars, light yellow cleistothecia, broadly lenticular ascospores with two conspicuously serrate-incised equatorial crests and tuberculate convex surfaces, and globose to subglobose conidia with a smooth wall. Neosartorya tsurutae is characterized by its spreading growth on Czapeks and malt extract agars, white cleistothecia, broadly lenticular ascospores with four equatorial crests and rugulose-ruminate convex surfaces, and ovoid to broadly ellipsoidal conidia with a smooth wall.
  Mycoscience 01/2003; 44(5):397-402. · 1.21 Impact Factor
• Article: Identification of the first isolates of Trichosporon asahii var asahii from disseminated trichosporonosis in China.

  Paride Abliz, Kazutaka Fukushima, Kayoko Takizawa, Rongya Yang, Ruoyu Li, Kazuko Nishimura
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  ABSTRACT: Infection with Trichosporon asahii is a major cause of deep-seated and disseminated trichosporonosis, which is associated with a high mortality rate. Disseminated trichosporonosis in individuals with no underlying disease has not been reported. In this study, we report the identification of the first isolate of Trichosporon asahii var. asahii in China. Two isolates were obtained from the liver and skin of a patient with disseminated trichosporonosis who displayed no evidence of underlying disease. The morphologic and physiologic characteristics of the two isolates differed slightly from those of usual strains of T. asahii var. asahii, including the type strain CBS 2479. Ubiquinone-9 was identified as the major ubiquinone in both isolates. Sequence analysis of the LSUrDNA D1/D2, ITS, and IGS1 regions from the two isolates showed them to be T. asahii var. asahii, and random amplified polymorphic DNA analysis strongly suggested that they were the same strain.
  Diagnostic Microbiology and Infectious Disease 10/2002; 44(1):17-22. · 2.53 Impact Factor
• Article: The first isolation of Hortaea werneckii from a household guinea pig.

  Shahana Sharmin, Kumiko Haritani, Reiko Tanaka, Paride Abliz, Kayoko Takizawa, Ayako Sano, Kazutaka Fukushima, Kazuko Nishimura, Makoto Miyaji
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  ABSTRACT: Hortaea werneckii, a black yeast and the causative agent of tinea nigra (a superficial type of dermatomycosis), is a human pathogen and is also found in the environment. It is not highly pathogenic but in the last fifteen to twenty years has been isolated from various human and environmental sources in Japan. As far as we know, there has been no report on the isolation of H. werneckii from animals. Recently, we found a case of a guinea pig with dark superficial lesions on the palm and dorsal areas. Cultural and morphological studies of scrapings from the lesion showed that the causative agent was a black yeast, which was identified as H. werneckii by morphological study and molecular biological screening. D1/D2 region of the 26S large subunit rDNA gene of this isolate was identical to those of 11 other H. werneckii isolates used as reference strains in this study. This is the first case recorded of tinea nigra caused by H. werneckii in a guinea pig.
  Nippon Ishinkin Gakkai Zasshi 02/2002; 43(3):175-80.
• Source

  Available from: aspergillus.org.uk

  Article: New and interesting species ofEurotium from Chinese soil

  Paride Abliz, Yoshikazu Horie, Yan Hui, Kazuko Nishimura, Ruoyu Li
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  ABSTRACT: Eurotium taklimakanense, a new species isolated from desert soil in the Taklimakan desert, Xinjiang Province, China, is described and illustrated. It is characterized by light yellow to reddish yellow colonies on Czapek's agar with 70% (w/v) sucrose, yellow ascomata, broadly lenticular ascospores with conspicuously irregular equatorial crests and tuberculate or verrucose convex surfaces, and anAspergillus anamorph.Eurotium cristatum is also described as a new record from China. It is characterized by light yellow colonies on Czapek's agar with 70% sucrose, yellow ascomata, broadly lenticular ascospores with two equatorial crests and echinulate convex surfaces, and small tuberculate conidia.
  Mycoscience 01/2001; 42(3):289-294. · 1.21 Impact Factor
• Article: Neosartorya takakii , a new species from soil in Brazil

  Yoshikazu Horie, Paride Abliz, Kazutaka Fukushima, Kaoru Okada, Norma B. Gusmão
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  ABSTRACT: Among the isolates from soil of grassland in Roraima State, Brazil, a new species ofNeosartorya, N. takakii is described and illustrated.Neosartorya takakii differs from the other known species of the genus in having lenticular ascospores with two distinct equatorial crests and with roughly circularly arranged projections on the convex walls. A comparative SEM view of ascospores from six relatedNeosartorya species is also provided.
  Mycoscience 01/2001; 42(1):91-95. · 1.21 Impact Factor
• Article: Emericella qinqixianii, a new species from desert soil in China

  Yoshikazu Horie, Paride Abliz, Yan Hui, Toshimitsu Fukiharu, Kazuko Nishimura, Dong Mei Li, Ruoyu Li
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  ABSTRACT: Emericella qinqixianii, a new species isolated from desert soil from Sanchakou, Aksu, Qiemo, Yuli, Yutian, and the Taklimakan desert 100 km inland from Minfeng, Xinjiang Province, China, is described and illustrated. It is characterized by grayish yellow to olive brown, non-ostiolate ascomata surrounded by hyaline to pale yellowish brown hülle cells, membranaceous peridium, prototunicate asci, and violet-brown, lenticular ascospores with two equatorial crests, smooth convex surfaces, and long filiform appendages. It hasAspergillus anamorph with biseriate aspergilla.
  Mycoscience 03/2000; 41(2):183-187. · 1.21 Impact Factor
• Article: Emericella appendiculata, a new species from Chinese soil

  Yoshikazu Horie, Dong Mei Li, Toshimitu Fukiharu, Ruoyu Li, Paride Abliz, Kazuko Nishimura, Duan Li Wang
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  ABSTRACT: Emericella appendiculata, a new species isolated from soil of the Pamire Plateau, is described and illustrated. It is characterized by grayish green non-ostiolate ascomata surrounded by a thick layer of hülle cells, membranaceous peridium, prototunicate asci, violet-brown, lenticular ascospores which are ornamented by two stellate equatorial crests, capitate convex surfaces, and long filiform appendages, and anAspergillus anamorph with biseriate conidiogenous cells.
  Mycoscience 04/1998; 39(2):161-165. · 1.21 Impact Factor