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ABSTRACT: Currently, nanobioceramics have received much attention due to their potentially high biological performance. In the present study, the interactions between proteins and two types of hydroxyapatite (HA) ceramic particles with distinct microstructures were studied in vitro. Protein adsorption on the microwave sintered and conventionally sintered HA ceramic particles (named as HAMS and HACS, respectively) were carried out in simulated body fluid (SBF) containing model proteins or rat serum and then subjected to protein quantitative evaluation, SDS-PAGE, and Western blotting analysis. The ceramic particles were characterized by nitrogen sorption, Hg penetration, zeta potential, and solubility analysis. It was found that HAMS with nanosized crystallites had greater specific surface area and pore volume and wider pore size distribution ranging from 0.02 to 2 μm than HACS. Although bovine serum albumin and lysozyme have different electrical properties in SBF, both the model proteins showed higher adsorption amounts per gram solid on HAMS than HACS, as could be ascribed to the contribution of the micropores structure of HAMS. Similarly, HAMS adsorbed more serum proteins per gram solid than HACS when incubating in rat serum, and here the surfaces of both particles were almost completely covered by serum proteins, leading to almost the same protein adsorption amounts per unit area solid of HAMS and HACS. SDS-PAGE patterns proved that HA ceramic particles had different binding capacities for different serum proteins and was not highly dependent on the concentrations of the competing protein components present in rat serum. Western blotting analysis confirmed the enhanced adsorption of fibronectin and vitronectin on HAMS, indicating that HAMS might have better bioactivity than HACS. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
Journal of Biomedical Materials Research Part B Applied Biomaterials 11/2011; · 2.15 Impact Factor
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04/2011; , ISBN: 978-953-307-191-6
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ABSTRACT: In order to enhance the ability of calcium phosphate-based biomaterials for bone defect repair, icariin (Ica), one natural product with ability of promoting osteoblasts differentiation in vitro and enhancing bone formation in vivo, was loaded into porous β-tricalcium phosphate ceramic (β-TCP) disks. The obtained Ica-loaded porous β-TCP ceramic (Ica/β-TCP) disks were characterized by SEM. The SEM photos indicated that the disks had porous structure and the surface morphology of the porous β-TCP ceramic (β-PTCP) disks had no obvious difference from the Ica/β-TCP disks. The Ica release curve of Ica/β-TCP disks showed a burst release during the first 1 day and the concentration of released Ica during the first 3 days had low cytotoxicity. The loading Ica in Ica/β-TCP disks hardly affected the attachment and morphology of Ros17/28 cells, however, the Ica/β-TCP disks were favorable to supporting the proliferation and differentiation of Ros17/28 cells better compared with the β-PTCP disks. There was plenty of bone-like apatite formed on the surface of Ica/β-TCP disks soaked in SBF solution for three days. After back intramuscular implantation of rats for three months, no obvious osteogenic evidence was detected in β-PTCP disks, but new bone formation was observed in Ica/β-TCP disks. Fibrous tissues and slight inflammatory reaction was also found in the Ica/β-TCP disks and β-TCP disks. Therefore, the loading Ica did not change the biocompatibility of β-TCP ceramic, but enhanced the bioactivity of β-TCP ceramic in vivo. The Ica/β-TCP ceramic had potential to be used for bone defect repair.
Journal of Materials Science Materials in Medicine 12/2010; 22(2):371-9. · 2.32 Impact Factor
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ABSTRACT: The biological performance of biomaterials is strongly influenced by their protein adsorption characteristics, which are related to the structures and properties of both the biomaterial and the protein. In the present study two groups of hydroxyapatite (HA) and biphasic calcium phosphate (BCP) ceramic powders were fabricated by different drying processes. The roles of the phase composition and microstructure of the powders in the adsorption of various model proteins were evaluated. The experimental results showed that BCP always had a higher ability to adsorb fibrinogen, insulin or type I collagen (Col-I) than HA. The microporosity and micropore size of the CaP particles also had a strong impact on their protein adsorption characteristics. HA and BCP particles with higher microporosities and/or more micropores >20 nm in diameter could adsorb more fibrinogen or insulin. However, amounts of adsorbed Col-I were largely unaffected by the microstructure of HA and BCP particles.
Acta biomaterialia 10/2009; 6(4):1536-41. · 3.98 Impact Factor
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ABSTRACT: Chondrogenic differentiation of mesenchymal stem cells (MSCs) relies on inductive media of chondrogenic environment. With proper design, a cellular microenvironment mimicking chondrogenic environment might be created to induce chondrogenesis of MSCs. In this study, bone marrow mesenchymal cells (BMSCs) were encapsulated in collagen-based hydrogel, and then enclosed in diffusion-chambers which allow the body fluid to permeate and preclude the host cells to invade. Then, the chamber with the hydrogel-BMSCs composite was implanted in the back of rabbits subcutaneously. The specimens in the chamber were harvested for histological, immunohistochemical, and RT-PCR analyses after 8 weeks. The results showed that cells with the characteristic of chondrocytes were homogenously distributed and the extracellular matrix (ECM) of cartilage has been secreted, indicating the chondrogenic differentiation of BMSCs. As control, nothing was obtained with only BMSCs. Moreover, the expression of collagen type II, indicator of cartilage ECM, was less in tissues with collagen-alginate-hydrogel (CAH) than that with collagen-hydrogel (CH). The results showed that both CH and CAH may induce the chondrogenesis and the induction is materials dependent. From in vitro experiments, TGF-beta is a necessary signal molecule for chondrogenesis, and it was suggested that the material may take in vivo growth factors to trigger chondrogenesis. From the studies, the chondrogenic induction of the hydrogel may be ascribed to that the hydrogel may provide a suitable environment and aggregate the signal molecule for chondrogenesis in vivo. The results would lend valuable reference in clinical for selection of appropriate scaffold for cartilage repair.
Journal of Biomedical Materials Research Part A 09/2009; 93(2):783-92. · 2.63 Impact Factor
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ABSTRACT: Protein adsorption affects the function of cells and determines the bioactivity of biomaterial implants. Surface structure and properties of materials determine the behavior of protein adsorption. In the present study, two biphasic calcium-phosphate ceramics (BCPs) with different surface structures were fabricated by pressing and H2O2 foaming methods. Their surface characteristics were analyzed and the in vitro and in vivo protein adsorption on them was investigated. Porous BCP showed higher ability to adsorb proteins, and transforming growth factor-beta1 (TGF-beta1) adsorption notably increased with increasing in vivo implantation time. The strong affinity of BCP to TGF-beta1 might provide important information for exploring the mechanism of the osteoinduction of calcium phosphates.
Acta biomaterialia 01/2009; 5(4):1311-8. · 3.98 Impact Factor
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ABSTRACT: The aim of this research is to study the effect of the controlled releasing character of the salmon calcitonin (S-CT) loaded injectable calcium phosphate cement (CPC) modified by adding organic phase, chitosan oligosaccharide (CO) and collagen polypeptide (CP). The uniform design was used to determine the basic formulation with suitable injectable time for clinical application, and then the changes of the physical characters, the controlled releasing character of the modified CPC along with the ratio of the organic phase were also evaluated in vitro. The surface morphous of the modified CPC been implanted in the abdominal cavity or soaked into the serum of rat was also observed by scanning electron microscope (SEM). The result shows that a suitable formulation of modified CPC could be got, and the injectable time is 12 min, the compressive strength is 12 MPa, and the final setting time is 40 min. Comparing with the CPC without organic phase, the releasing rate of S-CT would increase along with the increase of the organic phase after 7th day. Therefore, a novel S-CT loaded bioactive injectable CPC for treating osteoporosis induced bone defect was obtained, and the release of the containing S-CT was controlled easily through adjusting the ratio of CO and CP.
Journal of Materials Science Materials in Medicine 12/2007; 18(11):2225-31. · 2.32 Impact Factor
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ABSTRACT: Characterizations of hydroxyapatite (HA), biphasic calcium phosphate (BCP) and beta tricalcium phosphate (beta-TCP) ceramic particles were carried out using X-ray diffusion (XRD), Scanning electron micrograph (SEM), Particle Sizer and Zeta potential analyzer. Competitive adsorption of bovine serum albumin (BSA) and lysozyme (LSZ) on the three calcium phosphates were investigated by polyacrylamide gel electrophoresis (PAGE) method. The results showed that HA, BCP and beta-TCP ceramic particles with irregular shapes and similar size distributions all had negative surface net charges in pH7.4 phosphate buffered saline (PBS) solution and exhibited alike behaviors of BSA and LSZ adsorption. LSZ had higher affinity for calcium phosphate ceramics than BSA and its adsorption on them didn't be almost influenced by the increasing of BSA concentration in the solution. Electrostatic interaction played an important role on the competitive adsorption of BSA and LSZ on the surface of calcium phosphate ceramic particles.
Journal of Materials Science Materials in Medicine 12/2007; 18(11):2243-9. · 2.32 Impact Factor
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ABSTRACT: The biological performance of biomaterials is strongly influenced by their protein adsorption characteristics, which are related to the structures and properties of both the biomaterial and the protein. In the present study two groups of hydroxyapatite (HA) and biphasic calcium phosphate (BCP) ceramic powders were fabricated by different drying processes. The roles of the phase composition and microstructure of the powders in the adsorption of various model proteins were evaluated. The experimental results showed that BCP always had a higher ability to adsorb fibrinogen, insulin or type I collagen (Col-I) than HA. The microporosity and micropore size of the CaP particles also had a strong impact on their protein adsorption characteristics. HA and BCP particles with higher microporosities and/or more micropores >20 nm in diameter could adsorb more fibrinogen or insulin. However, amounts of adsorbed Col-I were largely unaffected by the microstructure of HA and BCP particles.
Acta Biomaterialia.
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[show abstract]
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ABSTRACT: Protein adsorption affects the function of cells and determines the bioactivity of biomaterial implants. Surface structure and properties of materials determine the behavior of protein adsorption. In the present study, two biphasic calcium-phosphate ceramics (BCPs) with different surface structures were fabricated by pressing and H2O2 foaming methods. Their surface characteristics were analyzed and the in vitro and in vivo protein adsorption on them was investigated. Porous BCP showed higher ability to adsorb proteins, and transforming growth factor-β1 (TGF-β1) adsorption notably increased with increasing in vivo implantation time. The strong affinity of BCP to TGF-β1 might provide important information for exploring the mechanism of the osteoinduction of calcium phosphates.
Acta Biomaterialia.
