Shan-yi Qiao

Academy of Military Medical Sciences, T’ien-ching-shih, Tianjin Shi, China

Are you Shan-yi Qiao?

Claim your profile

Publications (14)9.33 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: The chromatographic fingerprint was established by eluting with the mobile phase consisted of acetonitrile and 0.2% formic acid water on an Agilent TC-C18 (2) column (4.6 mm x 250 mm, 5 microm). Six chromatographic peaks were identified by HPLC-MS/MS method. Ten batches of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle were determined, and the similarity was arranged from 0.72 to 0.99. Good precision, stability and repeatability were obtained, and this study provides a reference for the quality control of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle.
    06/2014; 39(11):2056-9.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Previous studies showed that Danggui-Shaoyao-San (DSS), a traditional Chinese medicinal prescription, could alleviate cognitive dysfunction of Alzheimer's disease (AD) patients. However, the mechanisms remain unclear; we have now examined the effect of DSS on SAMP8 and elucidated the possible mechanism. Animals were treated with DSS for 2 months, and step-down test and Morris water maze (MWM) test were used to evaluated cognitive abilities. The estradiol (E2), NO, and glycine in blood plasma or in hippocampus were detected to explore the possible mechanisms. The latency of SAMP8 in step-down test was shorter than that of age-matched SAMR1, and DSS increased the latency especially in female animals. In MWM test, we got similar results; SAMP8 spent more time to find the platform, and DSS decreased the time before finding the platform, with little effect on swim velocity, during the training sessions. During test session, DSS increased the time spent in target quadrant especially in female SAMP8. In female SAMP8, plasma E2, NO, and glycine were elevated in plasma or hippocampus tissue. In conclusion, DSS could ameliorate deterioration of cognition in SAMP8, especially in female animals. Increasing E2, NO, and glycine might contribute to the cognitive improvement effect of DSS in female SAMP8.
    Evidence-based Complementary and Alternative Medicine 01/2014; 2014:327294. · 1.72 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To establish HPLC fingerprints of Liuwei Dihuang condensed pills. Dikma Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) was adopted, with acetonitrile (containing 0.05% phosphoric) -water (containing 0.05% phosphoric) as the mobile phase. The column temperature was set at 40 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was 276 nm (0-10 min), 236 nm (10-40 min) and 276 nm (40-60 min). The sample size was 20 microL. Chromatographic peaks were identified by Q-TOF-MS-IDA-MS/MS method. Good precision, stability and repeatability were proved. Q-TOF-MS-IDA-MS/ MS method was adopted for qualitative determination of eighteen chromatographic peaks. Ten batches of Liuwei Dihuang condensed pills were determined with the method, and their similarities were above 0. 96. The study lays a foundation for the overall quality evaluation of Liuwei Dihuang condensed pills.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 11/2012; 37(22):3411-5.
  • Xiao-yan Wang, Lei Sun, Shan-yi Qiao
    [Show abstract] [Hide abstract]
    ABSTRACT: To study chemical constituents of glucoside fraction from Liuwei Dihuang Gantang and clarify its substance foundation of active constituents. Glucoside fraction was prepared by macroporous resin chromatography. Constituents were separated by silica gel and reverse phase silica gel column chromatography, and their structures were identified by MS and NMR. Eleven compounds were separated and identified as 7-dehydrologanin (1), 7alpha-O-methylmorroniside (2), 7beta-O-methylmorroniside (3), 7alpha-O-ethylmorroniside (4), 7beta-O-ethylmorroniside (5), morroniside (6), sweroside (7), loganin (8), paeoniflorin (9), paeonolide (10) and loganic acid (11). All of those compounds were separated from Liuwei Dihuang Gantang for the first time.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 09/2012; 37(17):2576-80.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: JD-30 is an active fraction extracted from Danggui-Shaoyao-San (DSS), a traditional Chinese medicinal prescription. We previously showed that JD-30 could alleviate cognitive dysfunction of the mice induced by intracerebroventricular injection of β-amyloid (Aβ). However, data remain scarce on the effect of JD-30 on an Alzheimer's disease (AD) model and the underlying mechanisms are unknown. Further detailed studies on the effects of JD-30 on spatial cognition of senescence-accelerated mouse prone 8 (SAMP8), a suitable rodent model for cognitive impairment of aged subjects were investigated to elucidate the possible mechanisms. Long-term treatment with JD-30 significantly decreased the prolonged latency of SAMP8 in the Morris water-maze test. It also ameliorated the reduction of long-term potentiation (LTP) and reduced the damage of neurons in the hippocampus of SAMP8. Finally, JD-30 decreased the content and deposition of Aβ in the brain of SAMP8. The results show that JD-30 improves deterioration of spatial learning and memory in the SAMP8 mouse model, and by decreasing the content and deposition of Aβ, neuronal activity and synaptic plasticity improve, suggesting one of the mechanisms involved.
    Experimental gerontology 10/2011; 47(1):14-22. · 3.34 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Previous studies showed that Danggui-Shaoyao-San (DSS), a traditional Chinese medicinal prescription, could alleviate cognitive dysfunction of Alzheimer's disease (AD) patients. However, the mechanism and substance basis remain unknown. JD-30 is a fraction extracted from DSS, whose activity we previously was evaluated. beta-Amyloid (Abeta) is believed to be a critical etiological factor of AD. We have now examined the effect of DSS and JD-30 on AD model mice induced by Abeta, and elucidated the possible mechanism. Mice were intracerebroventricular injected with the aggregated Abeta(25-35) to mimic AD. Groups of mice were treated with DSS or JD-30 by intragastric infusion over 2 weeks, and their spatial learning and memory capacities were measured by the Morris water maze procedure. The mechanisms were investigated by extracellular microelectrode recordings, and also electron microscopy. Our results show that Abeta(25-35) induced impairment of spatial learning and memory in mice, as well as inhibition of long-term potentiation (LTP) in the hippocampus. The impairments were ameliorated by DSS or JD-30 administration. Additionally, JD-30 not only prevented the aggregation of Abeta(25-35), but disrupted aggregated Abeta(25-35) fibrils. These results suggest that JD-30 is one of the chief active fractions extracted from DSS by its ability to ameliorate deterioration of cognition, and by blocking and disrupting the aggregation of Abeta so that synaptic plasticity was improved, which may be one of the mechanisms involved.
    Journal of ethnopharmacology 03/2010; 128(2):365-72. · 2.32 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To develop an HPLC method for the determination of eleutheroside B in As1 (the extract fraction from Acanthopanax senticosus, which has a good effect of antifatigue). The antifatigue effect of Asl was evaluated by mice burden swimming testing. One compound was isolated by silica gel, Sephadex LH-20 column chromatography and preparative high performance liquid chromatography. The structure was identified by physicochemical properties and spectral evidences. Eleutheroside B in Asl was determined by HPLC. Chromatographic conditions included Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) and the mobile phase consisting of a mixture of acetonitrile-water-ethanoic acid (10: 90: 0.01). The UV detection wavelength was set at 344 nm. As1 showed an excellent antifatigue activity; One compound was isolated from As1 and its structure was identified as Eleutheroside B; The calibration cure was linear in the range of 0.104-20.8 microg (r = 0.9999), the average recovery was 97.68%, RSD 1.4% (n=6). This HPLC method is simple,accurate and reliable.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 01/2009; 33(23):2800-2.
  • [Show abstract] [Hide abstract]
    ABSTRACT: To study the chemical constituents of an active fraction (DSS-A-N-30) from Danggui Shaoyao San. DSS-A-N30 was prepared by macroporous resin chromatography, the compound was isolated by column chromatography on silica gel and RPC-18, the structure was elucidated by spectroscopic methods. A new monoterpene glycoside was isolated and identified from DSS-A-N-30. The new monoterpene glycoside was identified as 4"-hydroxyl-albiflorin.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 07/2008; 33(12):1413-5.
  • [Show abstract] [Hide abstract]
    ABSTRACT: To search the anti-inflammatory fraction of Albizia julibrissin. Inflammatory model of Kunming mice ear edema induced by croton oil and determination combined with the LC-MS-MS-guided fractionation and isolation were used. The n-butanol fraction (AJ-B) obtained from the ethanolic extract of the Cortex albiziae was the major active fraction. The lignan glycosides fraction (AJ-B-1), which was further isolated from AJ-B, showed significant anti-inflammatory activity and exhibited dose-dependent relationship in the dose of 5 to 20 mg x kg(-1). The method of bioassay-guided fractionation and isolation combined with the LC-MS-MS determination may be of benefit to the logical studies on the bioactive fractions or constituents of traditional Chinese materia medica.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 11/2007; 32(19):2021-5.
  • Qi Dong, Yuan Huang, Shan-yi Qiao
    [Show abstract] [Hide abstract]
    ABSTRACT: To study the chemical constituents of Stellaria media. The compounds were isolated with various column chromatography and semi-preparative HPLC. The structures were determined by modem spectroscopic methods (IR, UV, 1H-NMR, 13C-NMR, HMBC, ESI-MS). Five compounds were isolated and identified as apigenin 6-C-beta-D-galactopyranosyl-8-C-alpha-L-arabinopyranoside (1), apigenin 6-C-alpha-L-arabinopyranosyl-8-C-beta-D-galactopyranoside (2), apigenin 6-C-beta-D-galactopyranosyl-8-C-beta-L-arabinopyranoside (3), apigenin 6-C-beta-D-glucopyranosyl-8-C-beta-D-galactopyranoside (4), apigenin 6, 8-di-C-alpha-L-arabinopyranoside (5). Compounds 1-5 were obtained from Stellaria genus for the first time.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 07/2007; 32(11):1048-51.
  • Wei-wei Shang, Shan-yi Qiao
    [Show abstract] [Hide abstract]
    ABSTRACT: As a traditional Chinese medicine, Danggui Shaoyao powder has been studied for more than one thousand years. This article reviews the recent advances in chemical constituents, pharmacological activities and clinical application.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 05/2006; 31(8):630-3.
  • [Show abstract] [Hide abstract]
    ABSTRACT: A sensitive liquid chromatographic-tandem mass spectrometry(LC-MS/MS) method was developed for the determination of rizatriptan in human plasma. The analytes were extracted from plasma samples by liquid-liquid extraction, separated on a Zorbax XDB C8 column (150 x 4.6 mm i.d.) and detected by tandem mass spectrometry with an electrospray ionization interface. Zomitriptan was used as the internal standard. The method had a lower limit of quantitation of 50 pg/mL for rizatriptan, which showed more sensitivity and speed of analysis compared with reported methods. The within- and between-day precision was measured to be below 11.71% and accuracy between -5.87 and 0.86% for all quality control samples. This quantitation method was successfully applied to the evaluation of the pharmacokinetic profiles of rizatriptan after single oral administration of 5, 10 and 15 mg rizatriptan tablets to 10 healthy volunteers (five males and five females).
    Biomedical Chromatography 02/2006; 20(1):61-6. · 1.95 Impact Factor
  • Dong-hong Yu, Shan-yi Qiao, Yi-min Zhao
    [Show abstract] [Hide abstract]
    ABSTRACT: Cortex Albizziae, the stem bark of the leguminous plant Albizzia julibrissin, is specified in Chinese pharmacopoeia as a traditional Chinese medicine used to relieve melancholia and uneasiness of body and mind, invigorate the circulation of blood and subside a swelling. This article reviews the recent advances in chemical constituents and pharmacological activities of Cortex Albizziae.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 08/2004; 29(7):619-24.
  • [Show abstract] [Hide abstract]
    ABSTRACT: To establish a RP-HPLC method for determination of glycosides in Traditional Chinese Medicine Liu-wei Di-huang. The samples were analyzed on an ODS column at 30 degrees C, with mobile phase of methanol/water (33:67) at flow rate 1.0 mL.min and detection at wavelength of 236 nm. Three major components reached base-line separation and were identified to be mononiside, loganin, paeoniflorin. Respectively for the three components, linear correlations were found between peak areas and concentrations in the ranges of 7.4-60, 7.7-62 mg.L-1 and 8.5-68 mg.L-1, and the recoveries were 98.8%, 98.3%, 99.6%. The established method is proved to be suitable for simultaneous quantification of three major glycosidic components in Liuwei Dihuang decoction and can be used for evaluation of the quality of Liuwei Dihuang preparations.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 09/2003; 28(8):735-8.

Publication Stats

32 Citations
9.33 Total Impact Points

Top co-authors View all

Institutions

  • 2006–2012
    • Academy of Military Medical Sciences
      T’ien-ching-shih, Tianjin Shi, China
    • Government of the People's Republic of China
      Peping, Beijing, China
  • 2007–2009
    • Shenyang Pharmaceutical University
      Feng-t’ien, Liaoning, China