Michael F Angel

Southern Illinois University School of Medicine, Springfield, IL, United States

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Publications (39)48.76 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Preconditioning has been considered promising for the treatment of ischemic flaps. In this study, the therapeutic effect of postconditioning was compared with that of preconditioning during ischemia/reperfusion (I/R) injury, and a role of inducible nitric oxide synthase (iNOS) in postconditioning treatment was also explored. Sixty rats were randomly divided into four groups with 15 rats in each group. Ischemic injury was induced in a rat's gracilis muscle flap model. Preconditioning and postconditioning were performed respectively on the flaps in the pre-con group and the post-con group. No treatment was given to the flaps in the control group, and flaps without I/R injury were used as a sham control. Muscle viability ratio, histology, and gene expression of iNOS were examined at different time intervals (3, 12, and 18 h). A significantly higher survival ratio was observed in both the pre-con group (78.98 ± 3.39, 62.74 ± 3.7, and 54.42 ± 4.45 %) and the post-con group (77.42 ± 4.14, 59.74 ± 6.67, and 49.52 ± 4.13 %) than the control group (45.22 ± 3.69, 42.44 ± 3.76, and 33.2 ± 3.29 %) at 3, 12, and 18 h postoperatively (P < 0.05). There was no statistical difference between the pre-con group and the post-con group (P > 0.05). Histological examination showed delayed and attenuated tissue damage in both the pre-con group and the post-con group when compared to that of the control group. A higher expression of iNOS was observed in both the pre-con group and the post-con group than the control group and the sham group (P < 0.05). Significant improvement of flap survival could be achieved by both preconditioning and postconditioning treatments; however, better protection could be provided by preconditioning. The higher expression of iNOS may play an important role in the therapeutic effect of postconditioning during I/R injury.
    Hand 09/2012; 7(3):297-302.
  • Journal of oral and maxillofacial surgery: official journal of the American Association of Oral and Maxillofacial Surgeons 07/2012; 70(9):e509-15. · 1.58 Impact Factor
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    ABSTRACT: We describe a case of mutual transpositional transfers of toe and thumb in an attempt to restore the aesthetic appearance of the thumb with a deformity of the nail. The reconstructed thumb with the skin-nail flap from the great toe gave an excellent result both aesthetically and functionally, and the nail grew normally. The donor site of the great toe that was covered with the skin-nail flap from the thumb healed satisfactorily. This is another option for donor site management, the results of which are good, but the indications are under discussion.
    Journal of plastic surgery and hand surgery. 07/2012; 46(3-4):212-4.
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    ABSTRACT: The goals of this study were to review the outcome of the surgical procedure and hospitalization associated with meningomyelocele repair, and to examine the results of different closure strategies. Eighty-three consecutive patients having surgery for meningomyelocele over a ten year period form the basis of this study. Thirty-two closures with a mean defect size preoperatively of 11.5 cm(2) were performed by the neurosurgeon (ADP), and fifty-one closures with a mean defect size of 28.4 cm(2) by the plastic surgeon (MFA). Defects up to 12 cm(2) were closed with local advancement fasciocutaneous flaps. As defect size increased, latissimus muscle flaps were added in 30 (36%) and gluteus muscle in 16 (19%). In recent years, 18 patients (21.6%) with a mean defect of 29 cm(2) were treated with overlapping of deepithelialized fasciocutaneous flaps to add an additional layer of coverage to the dural closure. There were 9 major complications, 6 requiring reoperation. There were 10 minor wound failures managed conservatively. Mean hospital stay was 24.2 days. Re-operation increased length of stay to 45 days (p < 0.0001). Minor wound problems added 6 days to mean hospital stay. Wound failure did not correlate with either defect size or closure technique. Thoracic location was associated with increased wound failure (p < 0.05). Use of a shunt did not increase morbidity. All closures remained durable after discharge. Location in the thoracic area predicts major wound failure and need for reoperation. Wound complications significantly increase hospital stay. The use of a variety of techniques to achieve multi-layered closures leads to durable coverage for defects of all sizes.
    Journal of Plastic Reconstructive & Aesthetic Surgery 02/2012; 65(5):572-7. · 1.44 Impact Factor
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    ABSTRACT: The goal of this study was to evaluate the 4-year minimum (5.5-y average) results of trapeziectomy and ligament reconstruction using a modified Thompson technique with the abductor pollicis longus tendon for the primary treatment of advanced-stage basal joint arthritis (Eaton stages III and IV). We evaluated 25 thumbs in 18 patients after ligament reconstruction arthroplasty for surgical treatment of advanced thumb basal joint arthritis. Treatment consisted of piecemeal excision of the entire trapezium, ligament reconstruction and interposition using the abductor pollicis longus tendon, and 8 weeks of K-wire immobilization of the thumb metacarpal. We evaluated range of motion, lateral pinch, tip pinch, grip strength, and outcomes questionnaires including the Arthritis Impact Measurement Scales 2 Short Form before and at an average of 5.5 years after surgery. Seventeen of 18 patients reported excellent or good relief of pain and were satisfied with their operation, and all of the patients would have the operation again. Of the 25 thumbs, 24 adducted fully into the plane of the palm and opposed to the fifth metacarpal head. Preoperative and postoperative strength comparisons demonstrated an average increase in grip, key pinch, and tip pinch strength of 14%, 12%, and 6%, respectively. The outcomes data demonstrated noteworthy improvement in writing, buttoning a shirt, turning a key/lock, and arthritis pain categories. This technique restored a stable, pain-free thumb that yielded excellent strength and motion at an average of 5.5 years after the procedure. Compared with published reports of techniques that use hematoma distraction or harvest of all or part of the flexor carpi radialis tendon, this modified Thompson technique has similar pain relief, satisfaction, and motion but had less improvement in strength, which might have resulted from differences in the studied samples.
    The Journal of hand surgery 06/2011; 36(8):1326-32. · 1.33 Impact Factor
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    ABSTRACT: Purpose Long-term (66-month) clinical results of trapeziectomy and ligament reconstruction using the APL tendon in a modified Thompson technique, are not well known. Advantages of this technique include: retaining the FCR tendon as an important wrist stabilizer for pinch and grip, converting the APL tendon from a potential destabilizing to a stabilizing force, and avoidance of flexor surface incisions on the forearm. The goal of this study was to evaluate the results of this technique with an average 5.5-year follow-up for the primary treatment of advanced-stage basal joint arthritis (Eaton stages III and IV). Methods The senior author has performed 210 thumb basal joint arthroplasties using a modified Thompson technique. This was an independent pilot study to evaluate long-term results. Twenty-five thumbs in 18 patients from a single surgeon's practice were evaluated after ligament reconstruction arthroplasty for surgical treatment of advanced thumb basal joint arthritis. Treatment consisted of piecemeal excision of the entire trapezium, ligament reconstruction and interposition using the APL tendon, and 8 weeks of K-wire immobilization of the thumb metacarpal. Patients were seen, examined, and queried regarding their thumbs. Range of motion, lateral pinch, tip pinch, grip strength, and outcomes questionnaires including the Arthritis Impact Measurement Scales 2 Short Form (AIMS2-SF) were evaluated at an average of 66 months after surgery. Results At 5.5 years follow-up, 17 of 18 patients (94%) experienced “excellent” or “good” relief of pain and were “very satisfied” with their operation(s). Given identical preoperative circumstances, 18/18 (100%) patients would choose to have the operation again. Range of motion evaluation showed 24 of 25 thumbs adducted fully into the plane of the palm and 24 of 25 opposed to the fifth metacarpal head. Comparisons between preoperative and 66-month postoperative strength measurements demonstrated an average 13.8% increase in grip strength, 11.8% increase in key pinch strength, and a 6.3 % increase in tip pinch strength over preoperative values. Postoperative AIMS2-SF data demonstrated improvement in “Writing”, “Button shirt”, “Turn key/lock” and “Arthritis pain” categories compared with preoperative levels. Conclusions The modified Thompson technique restored a stable, pain-free thumb that yielded excellent strength and motion five and one-half years after the procedure. Compared with techniques that harvest all or part of FCR tendon, the modified Thompson method retains the entire FCR tendon for continued wrist stability and decreases the effect of APL tendon abduction force at base of thumb metacarpal for greater joint stability.
    AAHS ASPN ASRM 2011 Annual Meetings; 01/2011
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    ABSTRACT: Timely recognition of vascular compromise of free flaps is crucial to salvaging failing flaps due to the vulnerability of muscle tissues to ischemia. The concept of postconditioning (post-con) that has been introduced as an “after injury" strategy may be beneficial to salvage the failing muscle flaps. We aim to investigate the effect of post-con on the muscle flap survival after ischemia-reperfusion (I/R) injury in rats. The gracilis muscle flap model was used and a complete 4 hr of ischemia was generated by occlusion of the pedicle of dissected flap. The post-con procedure was started at the end of ischemia with six cycles of 15 s of reperfusion, followed by 15 s of complete reocclusion prior to the unlimited reperfusion. Muscle edema, malondialdehyde (MDA) level, muscle viability, and different time intervals (0, 3, 6, 18 hr) of gene expression of VEGF post-perfusion were assessed. Significant difference in muscle viability was noted between the post-con group and the control group (4 hr of ischemia followed by full reperfusion without intervention) in spite of being noncomparable with the sham group (no ischemic exposure) 3 days postoperatively. Statistically decreased muscle edema and MDA level were observed in the post-con group compared with the control group. Histological study also showed that attenuated inflammatory reaction was observed in the post-con group compared with the control group. A relatively higher level of VEGF since 3-hr post-reperfusion in the post-con group compared with the control and sham groups was recorded. Our results indicate that post-con procedure effectively reduces I/R injury and improves the survival of muscle flaps after ischemia. The consistent expression of VEGF in a high level may play an important role in the physiological effects of post-con.
    Journal of Investigative Surgery 10/2010; 23(5):249-56. · 1.32 Impact Factor
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    ABSTRACT: In recent years, the venous flap has been highly regarded in microsurgical and reconstructive surgeries, especially in the reconstruction of hand and digit injuries. It is easily designed and harvested with good quality. It is thin and pliable, without the need of sacrificing a major artery at the donor site, and has no limitation on the donor site. It can be transferred not only as a pure skin flap, but also as a composite flap including tendons and nerves as well as vein grafts. All these advantages make it an optimal candidate for hand and digit reconstruction when conventional flaps are limited or unavailable. In this article, we review its classifications and the selection of donor sites, update its clinical applications, and summarize its indications for all types of venous flaps in hand and digit reconstruction.
    Archives of Orthopaedic and Trauma Surgery 05/2010; 131(1):65-74. · 1.36 Impact Factor
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    ABSTRACT: The purpose of this experimental study is to investigate the improvement in flap survival of prearterialization with delay procedure in venous flaps in rats. The transverse superficial inferior epigastric flap was utilized. Forty-six rats were randomized into four groups: group 1 as arterialized venous flaps, group 2 as venous flaps of prearterialization with delay procedure, group 3 as arterial perfusion venous flap, and group 4 as venous perfusion venous flap. Direct observation, histological analysis, and vascular perfusion examination by Indian ink injection were performed for flap assessment. The percentage of flap survival was 41.6 +/- 2.4%, 98.0 +/- 1.8%, 89.5 +/- 1.0%, and 11.3 +/- 0.8% in these four groups, respectively. Significant differences were noted between groups ( P < 0.05) except for between group 2 and group 3 ( P > 0.05). Vascular perfusion studies revealed that the Indian ink filled the entire flaps of group 2 in comparison with partially filled flaps in other groups. Histological examination showed more small vessels were observed through all layers of the flaps as well as dilated superficial veins in group 2 than those in other groups. In conclusion, prearterialization with delay procedure can improve the viability of the flap, and this method may be a strategy for flap prefabrication based on the venous network.
    Journal of Reconstructive Microsurgery 04/2010; 26(3):193-200. · 1.00 Impact Factor
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    ABSTRACT: The arterialized venous flaps are highly regarded in microsurgical and reconstructive surgeries based on advantages of ease of design and harvest without the need to perform deep dissection, no sacrifice of a major artery at the donor site, no limitation of the donor sites, and less donor-site morbidity. Many experimental investigations and clinical applications have been reported. However, their survivals are still inconsistent, and survival mechanisms remain controversial. In this review, we update the existing problems, experimental studies for survival mechanisms, clinical practices, and methods developed to improve their survivals.
    Microsurgery 03/2010; 30(6):472-8. · 1.62 Impact Factor
  • Mehdi N. Adham, Andrew J. Kochevar, Michael F. Angel
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    ABSTRACT: An abstract is unavailable. This article is available as HTML full text and PDF.
    Plastic &amp Reconstructive Surgery 09/2009; 124(4S):66. · 3.54 Impact Factor
  • Andrew Kochevar, Ghazi Rayan, Michael Angel
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    ABSTRACT: To assess the feasibility of reconstructing extensor tendon segmental defects in zones II (over the middle phalanx) and IV (over the proximal phalanx) using local tendon flaps (LTFs), explore in these 2 zones the anatomical constraints that limit the use of the LTF as regards the maximum defect that could be reconstructed, and compare this flap with distant tendon grafts (DTG) reconstruction for similar size defects. We dissected 33 fresh-frozen cadaver extensor tendons from the fingers of 9 fresh-frozen cadaver forearms. A 0.5-cm defect was created in each extensor tendon of 21 fingers: 12 in zone II and 9 in zone IV. In each of 12 additional fingers, we created a 1.0-cm defect in zone IV. In 25 fingers, LTFs measuring 0.5 and 1.0 cm in length were harvested from the extensor tendon proximal to each defect and were turned distally to reconstruct the respective 0.5- and 1.0-cm defects. In 8 fingers, palmaris longus tendon grafts measuring 0.5 and 1.0 cm in length were used to reconstruct the respective 0.5- and 1.0-cm defects. Limited kinematic analysis was performed on the repaired fingers by maximally flexing the metacarpophalangeal, proximal interphalangeal, and distal interphalangeal joints in sequential fashion. In zone II, repair was technically feasible using LTFs in all 9 of the 0.5-cm extensor tendon defects. Likewise, LTFs were feasible for zone IV to repair 6 of 8 and all 9 of the respective 0.5- and 1.0-cm extensor tendon defects. Two failed repairs occurred early in the study by suture gapping following LTF of 0.5 cm to repair extensor tendon defects in zone IV of a long and small finger during maximal flexion. We determined the anatomical constraints for the use of the LTFs. The maximum length of repairable defect using the LTF was 0.5 cm in zone II of the index, long, ring, and small fingers, and zone IV of the small finger. In zone IV of the index, long, and ring fingers, the largest defect that could be repaired was 1.0 cm. Similarly, DTGs were feasible in zone II to repair all 4 of the 0.5-cm defects and in zone IV to repair all 4 of the 0.5- and 1.0-cm extensor tendon defects. In a cadaver model, both the LTF and the DTG are anatomically feasible and technically easy to perform. However, the LTF avoids a distant donor site, provides morphologically similar donor tendon that is readily accessible, and avoids morbidity that may be associated with the use of DTG. In this study, however, the LTF was limited in its use to zones II and IV of the extensor tendon.
    The Journal of hand surgery 07/2009; 34(7):1269-75. · 1.33 Impact Factor
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    ABSTRACT: In microsurgical flap procedures, creation of an arteriovenous fistula (AVF) is a technique of vein grafting where the vein graft is connected to recipient vessels as a flow-through loop prior to harvest and inset of the flap. Controversy exists whether this technique can be used as a 2-stage procedure with the loop and flap transfer accomplished in sequential operations or if the loop and flap transfer should be performed in a single operation. We performed 12 consecutive 1-stage AVF-flap procedures, with 1 flap failure. We combined this series with previously published reports to compare outcomes of 1-stage and 2-stage procedures. We found no significant difference in flap outcomes or complication rates between the 2 strategies. We conclude from our experience and this analysis that single-stage AVF-flap procedures are the optimum application of this technique.
    Annals of Plastic Surgery 02/2007; 58(1):61-3. · 1.38 Impact Factor
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    ABSTRACT: The radial forearm flap has been used for reconstruction of the cervical esophagus most often as a patch or for circumferential repair of short defects (less than 5 cm). In the following three cases, the radial forearm flap was used to reconstruct circumferential cervical esophageal defects ranging from 5 to 12 mm in length. These procedures were successfully carried out in three patients in whom the intraoperative defect was unexpectedly large or the intra-abdominal viscera harvest would have been difficult. These patients had reliable esophageal function 8 to 24 months after surgery. We conclude that the radial forearm flap can be used for reconstruction of large circumferential defects of the cervical esophagus.
    Journal of Craniofacial Surgery 04/2006; 17(2):382-7. · 0.69 Impact Factor
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    ABSTRACT: In cases of severe macromastia, the free nipple graft technique has been the traditional alternative to pedicle transposition. Distress over nipple survival in large reduction mammaplasty and long pedicle transposition is largely responsible for this.A retrospective investigation of the records of 142 reduction mammaplasty patients was carried out to determine whether nipple survival or overall complication rates were significantly different in patients undergoing larger (>1500 g per side) as compared with smaller reductions (< 1500 g per side). The 2 patient groups were compared with respect to mild or severe complications. Data were analyzed using Fisher exact test and 2-sample t tests. A P value of < 0.05 was considered statistically significant. No patient in either group had total nipple loss. There were no statistically significant differences in major or minor complications between the 2 groups. In our experience, the inferior pedicle, Wise pattern reduction is a reliable and predictable method of reduction, appropriate for all breast sizes and pedicle lengths.
    Annals of Plastic Surgery 10/2005; 55(3):245-9. · 1.38 Impact Factor
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    ABSTRACT: We investigated the changes in the properties of water when exposed to sunlight for 40 days. We hypothesize and prove that solar irradiation to water entraps electromagnetic radiation as potential energy, which becomes kinetic energy in various systems. It is postulated that photochemically-induced energy transfers, associated with individual spectral emission of visible spectrum of solar light, exert diverse influences on biological systems. Bottles of distilled water, individually wrapped in spectral-colored cellophane were exposed to sunlight and compared to an unwrapped bottle to determine chemical and physical changes as well as modifications of biological properties. Each bottle of water was named according to the color of cellophane paper with letter E (stands for exposed) as a prefix with (E-violet, E-indigo, E-blue, E-green, E-yellow, E-orange, and E-red). E-control (without wrap) was exposed to polychromatic sunlight. This study addresses two main issues viz., the chemical and physical changes in E-water and its effect on biological activities. Chemical and physical composition analysis using inductively coupled plasma atomic emission spectrometry; physical conductance by a Wheatstone Bridge type conductivity meter; osmolarity by a vapor pressure osmometer; and, salt solubility profile of 10% sodium bicarbonate were determined. Furthermore, testing the effect of E-waters on human lymphocyte proliferation, mosquito larvae hatching and seed germination determined the functional role of solar radiation through specific spectrum/s of visible light on various biological processes. We found that water exposed to visible spectral emissions of sunlight had an altered elemental composition, electrical conductance, osmolarity and salt-solubility, as well as differences in bio-modulatory effects. A gradual increase in leaching of Boron from E-violet to E-red was noted. E-indigo showed maximal increase in electrical conductance and maximal salt solubility of sodium bicarbonate. E-blue inhibited phyto-hemagglutinin-induced immune cell proliferation and mosquito larvae hatching. E-orange stimulated root elongation in seed germination. We conclude that 40-day exposure of water to specific solar spectrum changes chemical and physical properties and influences on biological activity.
    International journal of environmental research and public health 09/2005; 2(2):219-27. · 1.61 Impact Factor
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    ABSTRACT: In Eastern cultures, such as India, it is traditionally recommended that women but not men cover their heads while working in the scorching sun. The purpose of this pilot study was to determine whether there was any scientific basis for this cultural tradition. We examined the differential cytotoxic effects of ultraviolet A light (UVA) on an established T cell line treated with female and male sex hormones. CD4+ Jurkat T cells were plated in 96 well plates at 2 x 106 cells/ml and treated with 17beta-estradiol (EST) or testosterone (TE). These cells were irradiated by UVA light with an irradiance of 170 J/cm2 for 15min at a distance of 6 cm from the surface of the 96-well plate. Controls included cells not treated with hormones or UVA. The effects of EST and TE were investigated between 1 and 20 ng/mL. Cytotoxicity by fluorescein-diacetate staining and COMET assay generating single strand DNA cleavage, tail length and tail moment measurements were examined. The effect of estrogen (5ng/mL) on apoptosis and its mediators was further studied using DNA laddering and western blotting for bcl-2 and p53. We found that EST alone, without UVA, enhanced Jurkat T cell survival. However, EST exhibited a dose-related cytotoxicity in the presence of UVA; up to 28% at 20 ng/ml. TE did not alter UVA-induced cytotoxicity. Since TE did not alter cell viability in the presence of UVA further damaging studies were not performed. COMET assay demonstrated the harmful effects of EST in the presence of UVA while EST without UVA. had no significant effect on the nuclear damage. Apoptosis was not present as indicated by the absence of DNA laddering on agarose gel electrophoresis at 5ng/ml EST or TE +/- UVA. Western blot showed that estrogen down regulated bcl-2 independently of UVA radiation while p53 was down regulated in the presence of UVA treatment. EST and TE have differential effects on UVA-induced cytotoxicity in Jurkat T-lymphocyte which suggested that women may be more susceptible to the harmful effects of solar irradiation than men.
    International journal of environmental research and public health 05/2005; 2(1):156-63. · 1.61 Impact Factor
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    ABSTRACT: In Eastern cultures, such as India, it is traditionally recommended that women but not men cover their heads while working in the scorching sun. The purpose of this pilot study was to determine whether there was any scientific basis for this cultural tradition. We examined the differential cytotoxic effects of ultraviolet A light (UVA) on an established T cell line treated with female and male sex hormones. CD4+ Jurkat T cells were plated in 96 well plates at 2 x 106 cells/ml and treated with 17β-estradiol (EST) or testosterone (TE). These cells were irradiated by UVA light with an irradiance of 170 J/cm2 for 15min at a distance of 6 cm from the surface of the 96-well plate. Controls included cells not treated with hormones or UVA. The effects of EST and TE were investigated between 1 and 20 ng/mL. Cytotoxicity by fluorescein-diacetate staining and COMET assay generating single strand DNA cleavage, tail length and tail moment measurements were examined. The effect of estrogen (5ng/mL) on apoptosis and its mediators was further studied using DNA laddering and western blotting for bcl-2 and p53. We found that EST alone, without UVA, enhanced Jurkat T cell survival. However, EST exhibited a dose-related cytotoxicity in the presence of UVA; up to 28% at 20 ng/ml. TE did not alter UVA-induced cytotoxicity. Since TE did not alter cell viability in the presence of UVA further damaging studies were not performed. COMET assay demonstrated the harmful effects of EST in the presence of UVA while EST without UVA had no significant effect on the nuclear damage. Apoptosis was not present as indicated by the absence of DNA laddering on agarose gel electrophoresis at 5ng/ml EST or TE ± UVA. Western blot showed that estrogen down regulated bcl-2 independently of UVA radiation while p53 was down regulated in the presence of UVA treatment. EST and TE have differential effects on UVA-induced cytotoxicity in Jurkat T-lymphocyte which suggested that women may be more susceptible to the harmful effects of solar irradiation than men.
    International Journal of Environmental Research and Public Health. 01/2005;
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    ABSTRACT: The induction of endogenous vascular endothelial growth factor (VEGF) production in the skin flap with ischemic injury and the effect of exogenous VEGF on survival of the ischemic skin flap were studied in rats. A dorsal flap model (3x10 cm(2)) was used in this study. In Part I, biopsies were taken from the flap at 2.5, 5.5, and 8.5 cm distances from the distal edge at 0, 6, 12, and 24 h after the flaps were sutured. Malonyldialdehyde (MDA) and VEGF(165) protein level were measured. In Part II, exogenous VEGF (1 microg/ml) was injected subdermally into the flaps in 14 rats before the flaps were replaced. Flaps that received a saline injection were used as the controls. The skin paddle survival was measured on postoperative day five. The results showed that the MDA level in the distal part of the flap significantly increased at 24 h postoperatively when compared to MDA in other parts of the flap. However, VEGF levels in the distal part of the flap significantly decreased when compared to the middle part of the flap. Subdermal injection of exogenous VEGF to the distal area of the flap could significantly improve survival of the distal flap (89% of total skin paddle) when compared to the control, which had a 64% mean percent survival. We conclude that production of endogenous VEGF protein is significantly increased in the skin flap with mild ischemia, but decreased in the flap with severe ischemia. Administration of exogenous VEGF could significantly enhance survival of ischemic flaps.
    British Journal of Plastic Surgery 11/2003; 56(7):653-9. · 1.29 Impact Factor
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    ABSTRACT: The effects of static electromagnetic fields (SEFs) on MG-63, a human osteoblast cell-line, were investigated. We examined proliferation, proline uptake and gene expression in an SEF approximately 1/728th the intensity of those previously reported. Cells were placed within an SEF apparatus (average field intensity of 0.618mT) with appropriate controls. Proliferation was measured by 3H-thymidine incorporation and showed a 34% decrease in cells exposed to SEF (P = .0001; N = 3). Proline, a major component of collagen necessary for bone formation by osteoblasts, incorporation was reduced 37% (P = 0.006; N = 3). Reverse-transcription-polymerase chain reaction revealed that collagen I, alkaline phosphatase, parathyroid hormone-receptor, and osteocalcin mRNA's were down regulated with the low intensity SEF. Exposure to very low SEFs affects the MG-63 osteoblasts in a manner that may be detrimental to bone formation.
    Biomedical sciences instrumentation 02/2003; 39:454-9.

Publication Stats

303 Citations
48.76 Total Impact Points

Institutions

  • 2012
    • Southern Illinois University School of Medicine
      • Institute for Plastic Surgery
      Springfield, IL, United States
    • Wenzhou Medical College
      Yung-chia, Zhejiang Sheng, China
  • 2001–2010
    • University of Mississippi Medical Center
      • • Division of Plastic Surgery
      • • Department of Surgery
      • • Division of Rheumatology and Molecular Immunology
      Jackson, MS, United States
  • 2009
    • Oklahoma City University
      Oklahoma City, Oklahoma, United States
  • 2002
    • University of Virginia
      • Oral and Maxillofacial Surgery Clinic
      Charlottesville, Virginia, United States