Maria Söderlund-Venermo

University of Helsinki, Helsinki, Uusimaa, Finland

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Publications (98)377.72 Total impact

  • PLoS ONE 09/2015; 10(9):e0139096. DOI:10.1371/journal.pone.0139096 · 3.23 Impact Factor
  • Xuemeng Li · Kalle Kantola · Lea Hedman · Benedict Arku · Klaus Hedman · Maria Söderlund-Venermo
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    ABSTRACT: Human bocavirus (HBoV) 1 is a widespread parvovirus causing acute respiratory disease in young children. HBoV2 rather occurs in the gastrointestinal tract potentially associating with gastroenteritis, while HBoV3 and -4 infections are less frequent and not yet linked with human disease. Due to HBoV1-DNA persistence in the nasopharynx, serology has been advocated as a better alternative for diagnosing acute infections. In constitutionally healthy children, we have earlier noticed that pre-existing HBoV2 immunity in a subsequent HBoV1 infection typically resulted in low or non-existent HBoV1-specific antibody responses. A phenomenon describing such immunologic events among related viruses has been known since the 1950s as "original antigenic sin" (OAS). The aim of this study was to characterize this putative OAS phenomenon in a more controlled setting. Follow-up sera of 10 rabbit pairs, inoculated twice by HBoV1-4 virus-like particles (VLPs) or control antigens, in various combinations, were analysed with HBoV1-4 IgG EIAs with and without depletion of heterotypic HBoV antibodies. There were no significant IgG boosts after the second inoculation in either the heterologously or the homologously HBoV-inoculated rabbits, but a clear increase in cross-reactivity was seen with time. We could, however, distinguish a distinct OAS pattern from plain cross-reactivity: half the heterologously inoculated rabbits showed IgG patterns representative of the OAS hypothesis, in line with our prior results with naturally infected children.HBoVs are the first parvoviruses to show the possible existence of OAS. Our findings provide new information on HBoV1-4 immunity and emphasize the complexity of human bocavirus diagnosis.
    Journal of General Virology 07/2015; DOI:10.1099/jgv.0.000253 · 3.18 Impact Factor
  • A. Altay · T. Yahiro · G. Bozdayi · T. Matsumoto · F. Sahin · S. Ozkan · A. Nishizono · M. Söderlund-Venermo · K. Ahmed
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    ABSTRACT: Recently a parvovirus called bufavirus (BuV) has been implicated as a causative agent of diarrhoea. To further reveal the epidemiology and genetic characteristics of BuV this study was performed in Turkish children with diarrhoea. BuV was detected in 1.4% (8/583) of samples with diarrhoea. All stool samples from healthy children (n=148) were negative for BuV. Diarrhoea in BuV-positive patients was severe and occurred mainly during the colder months of the year. Complete genome sequences were generated from four BuVs. Only BuV3 was found, which were genetically and phylogenetically similar to Bhutanese BuV3 indicating that BuV3 is prevalent in Asian countries. Copyright © 2015. Published by Elsevier Ltd.
    Clinical Microbiology and Infection 06/2015; DOI:10.1016/j.cmi.2015.06.006 · 5.77 Impact Factor
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    M. Toppinen · P. Norja · L.-M. Aaltonen · S. Wessberg · L. Hedman · M. Söderlund-Venermo · K. Hedman
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    ABSTRACT: Parvovirus B19 (B19V) is a minute ssDNA virus associated with a wide range of diseases from childhood erythema to fetal death. After primary infection, the viral genomes persist lifelong in solid tissues of most types. Quantification of the viral DNA is important in the timing of primary infection, assessment of tissue persistence and screening of blood donor plasma. In this study, we present a new PCR assay for detection and quantification as well as for differentiation of all three B19V genotypes. A new B19V qPCR was designed to target a 154-bp region of the NS1 area. Serum, plasma and solid tissue samples were suitable for testing in the assay. The WHO International Reference Panel for Parvovirus B19 Genotypes was utilized to validate the assay for detection of different genotypes of B19V in clinical material. Each panel member yielded, by the new qPCR, a quantity similar to the one reported by National Institute for Biological Standards and Control (NIBSC). The qPCR was specific for B19V and amplified and quantified all three genotypes with detection sensitivities of ≤10 copies/reaction. The differentiation of B19V genotypes was performed by Sanger sequencing of the amplified products. Copyright © 2015. Published by Elsevier B.V.
    Journal of virological methods 03/2015; 218. DOI:10.1016/j.jviromet.2015.03.006 · 1.78 Impact Factor
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    ABSTRACT: Susceptibility to early rhinovirus-induced wheezing has been recognized as an important risk factor for childhood asthma, but data on the first wheezing episode are limited. The aim of this selected population study was to investigate virus etiology, atopic characteristics and illness severity, as well as their interrelation, among first-time wheezing children. We studied 111 first-time wheezing children aged between 3 to 23 months (88/23 in-/outpatients). The investigated factors included atopy, food, perennial and aeroallergen sensitization, eczema, atopic eczema, elevated blood eosinophil count, and parental allergic rhinitis, asthma and smoking. Nasopharyngeal aspirates were analyzed for adenovirus, coronaviruses, enteroviruses, bocavirus-1 (also serologically confirmed), influenza viruses, metapneumovirus, parainfluenza viruses, rhinovirus and respiratory syncytial virus using PCR methods. The mean age of the study patients was 12 months (standard deviation 6.0). Atopic characteristics could be found in 56%, atopic eczema in 16% and sensitization in 23% of the cases. In all samples (100%), >1 viruses were detected as follows: rhinovirus (76%), respiratory syncytial virus (29%), bocavirus (18%, acute infections), and other viruses <10% each. Virus coinfections occurred in 38% of the children. Rhinovirus infection was positively associated with age, blood eosinophil count, eczema and duration of cough, as well as parental allergic rhinitis and smoking but negatively associated with virus coinfection (all p < 0.05). A respiratory virus infection can be detected in all first-time wheezing children. Rhinovirus dominated the findings and was linked to atopic characteristics, prolonged cough and parental smoking. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Pediatric Allergy and Immunology 12/2014; 25(8). DOI:10.1111/pai.12318 · 3.40 Impact Factor
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    ABSTRACT: Vitamin D may be protective against acute respiratory infections and atopic illnesses, but studies have been conflicting (1, 2). One likely reason is the vast variability in measurement of vitamin D (based on interview about dietary intake versus actual measurement of 25-hydroxyvitamin D [25OHD] concentrations) and major differences in study populations (age, confirmation of atopy status, use of vitamin D supplements and latitude of study region). The recommended minimum level of serum 25OHD varies from 50 to 75 nmol/l (2, 3). This article is protected by copyright. All rights reserved.
    Pediatric Allergy and Immunology 11/2014; 25(8). DOI:10.1111/pai.12308 · 3.40 Impact Factor
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    ABSTRACT: Background Human parvovirus B19 (B19V) infection during early pregnancy increases the risk of miscarriage. Studies have inconsistently shown an elevated risk of infection among women with occupational contacts with children. Methodological differences, particularly in defining occupational exposure and in the type of reference group, may explain the conflicting findings. Methods This cohort study compared B19V infections in pregnant day-care employees and healthcare professionals during a B19V epidemic in Finland. Women were identified from the files of nationwide trade unions and the National Supervisory Authority for Welfare and Health. Early-pregnancy maternal B19V IgG was analysed in 3710 women, and infections were defined as seroconversions after analysing in parallel the available umbilical cord blood samples of the 847 seronegative mothers. Independently of the serological status, the actual employment during pregnancy was assessed using registered information on employment history. Results B19V infections were more common among day-care employees (22/331, 6.6%), than among those working in healthcare (12/326, 3.7%). The adjusted HRs of B19V infection, using proportional hazard regression, was 2.63 (95% CI 1.27 to 5.46) among all women and 5.59 (95% CI 1.40 to 22.4) among nulliparous women. Conclusions Day-care employees are at an increased risk of B19V infection, which warrants preventive measures.
    Occupational and Environmental Medicine 07/2014; 71(12). DOI:10.1136/oemed-2014-102217 · 3.27 Impact Factor
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    ABSTRACT: We identified a new genotype of bufavirus, BuV3, in fecal samples (0.8%) collected to determine the etiology of diarrhea in children in Bhutan. Norovirus GII.6 was detected in 1 sample; no other viral diarrheal pathogens were detected, suggesting BuV3 as a cause of diarrhea. This study investigates genetic diversity of circulating BuVs.
    Emerging infectious diseases 06/2014; 20(6):1037-9. DOI:10.3201/eid2006.131430 · 6.75 Impact Factor
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    Emerging infectious diseases 06/2014; 20(6):1078-80. DOI:10.3201/eid2006.131674 · 6.75 Impact Factor
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    ABSTRACT: We used comprehensive serodiagnostic methods (IgM, IgG, and IgG avidity) and PCR to study Merkel cell polyomavirus and trichodysplasia spinulosa-associated polyomavirus infections in children observed from infancy to adolescence. Comparing seroconversion intervals with previous and subsequent intervals, we found that primary infections with these 2 viruses were asymptomatic in childhood.
    Emerging Infectious Diseases 04/2014; 20(4):689-92. DOI:10.3201/eid2004.131015 · 6.75 Impact Factor
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    ABSTRACT: Unlabelled: Human bocaviruses (HBoVs) may be grouped into respiratory (HBoV1) and enteric (HBoV2-4) types. We examined this association of HBoV types and clinical symptoms in 955 children who had acute gastroenteritis (AGE, n = 172), acute respiratory tract infection (ARTI, n = 545) or symptoms of both (n = 238). Both nasal swab and stool specimens were studied for such patients. HBoV1 DNA was detected in 6.2 % of patients with ARTI and 9.2 % of patients with symptoms of both ARTI and AGE, but in only 1.7 % of patients with AGE alone. In about one half of the cases, HBoV1 was detected concomitantly in nasal swab and stool samples. HBoV2 was found in stool samples of patients with AGE (5.8 %), ARTI (5.1 %) and symptoms of both (5.5 %) but only rarely in nasal swabs. HBoV3 was found in the stools, but not in nasal swabs, in 0.6, 1.1 and 0.8 % of patients with, respectively, AGE, ARTI and both. HBoV4 was not found. All but one HBoV-positive stool sample of AGE patients contained a known gastroenteritis virus (rotavirus, norovirus, sapovirus, astrovirus or enteric adenovirus) that was probably responsible for the symptoms of the respective case. Sera of 30 HBoV-positive patients were available, and IgM antibodies for HBoVs were found in ten cases and HBoV DNA in eight of these. Conclusions: HBoV2 and HBoV3 were more commonly found in stool than in nasal swab samples, but the findings could not be causally linked with AGE. HBoV1 was commonly found in stool samples during ARTI, with or without gastrointestinal symptoms.
    European Journal of Pediatrics 03/2014; 173(8). DOI:10.1007/s00431-014-2290-x · 1.89 Impact Factor
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    The Journal of allergy and clinical immunology 01/2014; 133(1):256-258.e4. DOI:10.1016/j.jaci.2013.10.014 · 11.48 Impact Factor
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    ABSTRACT: B19V is a member of the Parvoviridae family, genus Erythrovirus. B19V IgG and IgM have different reactivities against conformational and linear epitopes of VP1 and VP2 antigens leading to the development of IgM, and IgG-avidity and epitope type specificity (ETS) enzyme immunoassays (EIAs) for identifying recent from past infection. Additionally, B19V viral load determination (qPCR) is increasingly used in staging B19V infection, and in this study the utility of these methods is compared.A panel of 78 sera was jointly tested by the Virus Reference Department (VRD), London, UK and the Haartman Institute (HI), Helsinki, Finland using a number of EIAs eg. B19V-IgG, IgM, IgG-avidity and ETS-EIA. At VRD, the sera were tested, also, by a B19V viral load PCR (qPCR).By consensus analysis, 43 (55.1%) sera represented past infection, 28 (35.9%) sera represented recent infection, and 7 (9.0%) sera were indeterminate. Both VRD B19V qPCR and HI B19V IgM EIA gave the highest agreement with consensus interpretation for past or recent infection with an overall agreement of 99% (95%CI:[92-100]) and positive predictive value (PPV) of 100% (95%CI:[87-100]). Nine sera, designated past infection by consensus analysis were B19V IgM positive by a commercial VRD B19V IgM EIA and B19V IgM negative by a new HI in house B19V IgM EIA. A new VRD IgG-avidity EIA showed good >95% agreement (excluding equivocal results) with consensus interpretations for recent or past infection.Correct identification of recent from past B19V infection was achieved through application of qPCR or by appropriate selection of EIAs.
    Journal of clinical microbiology 01/2014; 52(3). DOI:10.1128/JCM.02613-13 · 3.99 Impact Factor
  • The Journal of allergy and clinical immunology 12/2013; DOI:10.1016/j.jaci.2013.10.052 · 11.48 Impact Factor
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    ABSTRACT: The trichodysplasia spinulosa-associated polyomavirus (TSPyV), a recently discovered species of the family Polyomaviridae, is associated with development of trichodysplasia spinulosa (TS), a rare follicular skin disease of immunocompromised individuals. The viral seroprevalence in the general population is ∼70%, with little known of its route of transmission, latency, or primary infection site. We aimed to determine whether the viral DNA is detectable in tonsillar tissue of constitutionally healthy individuals, and what the corresponding antiviral seroreactivities are. We tested 229 matched pairs of tonsillar tissue biopsies and serum samples from asymptomatic donors for TSPyV DNA by real-time quantitative PCR with primer pairs and Taq-Man probes targeting the VP1 and LT genes. The sera were studied by enzyme immunoassay (EIA) for TSPyV-VP1-IgG and the PCR-positive individuals also for -IgM and -IgG-avidity. TSPyV DNA was detectable in 8 (3.5%) of 229 tonsillar tissues, and in none of the corresponding sera. TSPyV IgG seroprevalence among children was 39% and among adults 70%. Each of the 8 PCR-positive subjects had antiviral IgG of high avidity but not IgM. TSPyV PCR positivity of tonsillar samples of individuals with long-term immunity provides the first evidence of TSPyV in tonsils and suggests lymphoid tissue as a latency site of this emerging human pathogen.
    Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology 11/2013; 59(1). DOI:10.1016/j.jcv.2013.11.008 · 3.02 Impact Factor
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    ABSTRACT: We identified human bocavirus (HBoV) DNA by PCR in cerebrospinal fluid from adults and children with encephalitis in Sri Lanka. HBoV types 1, 2, and 3 were identified among these cases. Phylogenetic analysis of HBoV1 strain sequences found no subclustering with strains previously identified among encephalitis cases in Bangladesh.
    Emerging Infectious Diseases 11/2013; 19(11):1859-62. DOI:10.3201/eid1911.121548 · 6.75 Impact Factor
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    E Väisänen · A Lahtinen · A M Eis-Hübinger · M Lappalainen · K Hedman · M Söderlund-Venermo
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    ABSTRACT: Human parvovirus 4 (PARV4) of the family Parvoviridae was discovered in a plasma sample of a patient with an undiagnosed acute infection in 2005. Currently, three PARV4 genotypes have been identified, however, with an unknown clinical significance. Interestingly, these genotypes seem to differ in epidemiology. In Northern Europe, USA and Asia, genotypes 1 and 2 have been found to occur mainly in persons with a history of injecting drug use or other parenteral exposure. In contrast, genotype 3 appears to be endemic in sub-Saharan Africa, where it infects children and adults without such risk behaviour. In this study, a novel straightforward and cost-efficient molecular assay for both quantitation and genotyping of PARV4 DNA was developed. The two-step method first applies a single-probe pan-PARV4 qPCR for screening and quantitation of this relatively rare virus, and subsequently, only the positive samples undergo a real-time PCR-based multi-probe genotyping. The new qPCR-GT method is highly sensitive and specific regardless of the genotype, and thus being suitable for studying the clinical impact and occurrence of the different PARV4 genotypes.
    Journal of virological methods 10/2013; 195. DOI:10.1016/j.jviromet.2013.10.011 · 1.78 Impact Factor
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    ABSTRACT: Limited data are available on the effects of probiotics on the nasopharyngeal presence of respiratory viruses in children attending day care. In this substudy of a randomized, double-blinded, placebo-controlled 28-week intervention study, nasopharyngeal swab samples were collected, on visits to a physician due to symptoms of infection, from children receiving control milk (N = 97) and children receiving the same milk supplemented with probiotic Lactobacillus rhamnosus GG (N = 97). The presence of 14 respiratory viruses was assessed by PCR methods, and viral findings were compared with symptom prevalences in the intervention groups. Rhinovirus was identified in 28.6% of 315 swab samples, followed by respiratory syncytial virus (12.4%), parainfluenza virus 1 (12.1%), enterovirus (8.9%), influenza A(H1N1)pdm09 (7.9%), human bocavirus 1 (3.8%), parainfluenza virus 2 (3.2%), adenovirus (2.9%), and influenza A(H3N2) (0.6%). The children in the probiotic group had less days with respiratory symptoms per month than the children in the control group (6.48 [95% CI 6.28-6.68] vs. 7.19 [95% CI 6.98-7.41], P < 0.001). Probiotic intervention did not reduce significantly the occurrence of the examined respiratory viruses, or have an effect on the number of respiratory symptoms observed at the time of a viral finding. Rhinovirus, respiratory syncytial virus, and parainfluenza virus 1 were the most common respiratory viruses in symptomatic children. Children receiving Lactobacillus rhamnosus GG had fewer days with respiratory symptoms than children in the control group, although probiotic intervention was not effective in reducing the amount of viral findings or the respiratory symptoms associated with viral findings. J. Med. Virol. © 2013 Wiley Periodicals, Inc.
    Journal of Medical Virology 09/2013; 85(9). DOI:10.1002/jmv.23623 · 2.35 Impact Factor

Publication Stats

2k Citations
377.72 Total Impact Points


  • 2000–2015
    • University of Helsinki
      • Department of Virology
      Helsinki, Uusimaa, Finland
  • 2012
    • Universität Witten/Herdecke
      • Institute of Physiology and Pathophysiology
      Witten, North Rhine-Westphalia, Germany
  • 2003–2010
    • Helsinki University Central Hospital
      • Department of Medicine
      Helsinki, Southern Finland Province, Finland
  • 2008
    • Karolinska University Hospital
      • Department of Clinical Microbiology
      Tukholma, Stockholm, Sweden
  • 2005
    • University of Jyväskylä
      • Department of Biological and Environmental Science
      Jyväskylä, Province of Western Finland, Finland
  • 2002
    • University of Missouri
      • Department of Molecular Microbiology and Immunology
      Columbia, Missouri, United States