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ABSTRACT: ObjectiveTo identify metastasis-related biomarkers in human ovarian cancer cell lines and in serum.
MethodsWe isolated total protein from cell lysis solutions and cultured supernatants from 2 human ovarian cancer cell lines and used
SELDI-TOF-MS to detect the differential expression of the proteins in the 2 cell lines. The proteomic spectra were generated
using weak cation exchange chips. The biomarkers were validated by analyzing serum proteins or peptides in ovarian cancer
patients, relapsed ovarian cancer patients, patients with benign ovarian tumors, and healthy people.
ResultsFour proteins in the culture supernatant from HO-8910PM cells were up-regulated, relative to the culture supernatant of HO-8910
cells. One protein (3,144 Da m/z value) was up-regulated in both the cell lysis solution and in the culture supernatant of
HO-8910PM cells. In addition, expression of the 3,144 Da m/z protein differed significantly between serum from the 26 ovarian
cancer patients, from the 22 relapsed ovarian patients and from the 37 healthy women (P < 0.01). However, there was no difference between patients with benign ovarian tumors and healthy people (P > 0.5).
ConclusionOvarian cancer cell lines with high or low metastatic potential have distinct protein profiles. Protein 3,144 Da m/z could
be a useful biomarker for diagnosing ovarian cancer metastasis.
Clinical Oncology and Cancer Research 04/2012; 6(5):317-321.
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ABSTRACT: MicroRNAs (miRNAs) have been underlined as a promising potential biomarker for breast cancer but are limited to tissue specimens. Clinical specimens of sera are more abundant and more conveniently collected than tissues. This work was designed to investigate the expression and correlation of a selected panel of miRNAs associated with breast tumor in tissues and matching serum samples.
Tumor tissues, adjacent non-tumor tissues and matching serum samples were collected from 68 patients with newly diagnosed breast tumors. Normal control sera were collected from 40 healthy subjects. A panel of 6 miRNAs (miRNA-21, 106a, 126, 155, 199a and 335) were selected and their aberrant expression levels were quantified by using real-time PCR technique.
A high correlation of miRNA expression level was found between breast tumor tissues and sera. MiR-21, miR-106a and miR-155 were significantly over-expressed in the tumor specimens compared with those in normal controls (P < 0.05), whereas miR-126, miR-199a and miR-335 were significantly under-expressed (P < 0.05). Furthermore, the relative expression of miR-21, miR-126, miR-155, miR-199a and miR-335 was closely associated with clinicopathologic features of breast cancer (P < 0.05), such as histological tumor grades and sex hormone receptor expression.
Selective expression and modulation of miRNAs could be potential blood-based biomarkers for breast cancer diagnosis, grading and prognosis. Our results should encourage further studies on the use of miRNAs in serum samples as an easy and convenient method of breast cancer screening.
Gynecologic Oncology 12/2010; 119(3):586-93. · 3.89 Impact Factor
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Proceedings of the 2nd International Conference on BioMedical Engineering and Informatics, BMEI 2009, October 17-19, 2009, Tianjin, China; 01/2009
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Proceedings of the 2nd International Conference on BioMedical Engineering and Informatics, BMEI 2009, October 17-19, 2009, Tianjin, China; 01/2009
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ABSTRACT: Gene expression data analysis is a very useful tool for medical diagnosis. Combined with classification methods, this technology can be used to help make clinical decisions for individual patients. In this paper, a novel classification method for cancer microarray data was proposed. This method includes two stages: The first stage is to select a number of genes based on a gene selection algorithm, and then supervised locality preserving projections (SLPP) is accepted for further dimension reduction and discriminant feature extraction. This stage can find more discriminant projection direction based on training data. The second stage uses nearest neighborhood (NN) and support vector machine (SVM) for classification. To show the validity of the proposed method, 4 real cancer data sets were used for classifying. The prediction performance was evaluated by 3-fold cross validation. The experimental results show that the method presented here is effective and efficient.
Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on; 06/2008
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ABSTRACT: Analyzing microarray data to identify interesting genes is a well-established methodology but often results in inconsistent conclusions and even fails because of the variations of experimental conditions. This study proposes an across factor normalization based singular value decomposition approach to microarray data analysis. The approach has been applied to analyze gene expression profiles to identify differentially expressed genes associated with Ovarian Carcinoma chemotherapy responses. The influences of experimental conditions are identified by correlations analysis and Friedman'M test and illustrated by Scatter Plots. These influences are then removed by across factor normalization. Experimental results showed that after across factor normalization, the samples associated with different chemotherapy responses are significantly clustered together and genes linked to differential chemotherapy responses are identified.
Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on; 06/2008
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ABSTRACT: To analysis the change of Deinococcus radiodurans extracellular proteins recovering from gamma-irradiation, we examined extracellular proteome changes using two-dimensional polyacrylamide gel electrophoresis. Twenty-six spots on the gel of irradiated sample were showed significant changes compared with spots on the control gel. Using peptide mass fingerprinting via matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS), 21 different proteins could be distinguished. Among the identified proteins, seven are classified in transport and metabolism, and one is involved in intracellular trafficking and secretion. The other proteins are known to several functions in the cytosol. Most of the proteins have not previously been reported to be relevant to radioresistance. These results imply that the transmembrane transportation is involved in and contributes to the radioresistance in this organism.
Protein and Peptide Letters 02/2008; 15(6):595-9. · 1.94 Impact Factor
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ABSTRACT: A simple high-performance liquid-electrospray ionization tandem mass spectrometric (HPLC-ESI-MSn) method has been developed for the rapid identification of clindamycin and its related minor impurities in bulk drug. The ESI-MSn results obtained allowed us to propose plausible schemes for their fragmentations, which were confirmed further by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) using collision-induced dissociation (CID) method at high mass resolution. The positive ESI-MS/MS of clindamycin and its derivative compounds showed some diagnostic fragments, such as the neutral losses of H2O, HCl, methanethiol and 2-methylthio-ethenol, and the residue of 3-propyl-N-methylpyrrolidine and 3-ethyl-N-methylpyrrolidine, which are specific and useful for the identification of the lincosamide antibiotics and related impurities. According to the fragmentation mechanism of mass spectrometry and HPLC-UV-ESI-MSn data, six impurities of clindamycin have been identified on-line. Additionally, the positive ion mode extracted ion current (EIC) method has been used to separate and identify these lincosamide compounds.
Journal of Pharmaceutical and Biomedical Analysis 07/2006; 41(4):1116-23. · 2.97 Impact Factor
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ABSTRACT: Deinococcus radiodurans shows remarkable resistance to reactive oxygen species (ROS), generated by irradiation. Disruption of recX (dr1310) in D. radiodurans using targeted mutagenesis method enhanced its ROS scavenging activity, and recX overexpression in this bacterium repressed its antioxidant activity significantly. Further analyses on catalase and superoxide dismutase, two important antioxidant proteins in cells, showed that RecX could repress the induction of antioxidant enzymes, revealing that it negatively regulates the ROS scavenging activity in D. radiodurans.
FEMS Microbiology Letters 04/2005; 244(2):251-7. · 2.04 Impact Factor
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ABSTRACT: In order to reveal the mechanisms of the extreme radioresistance and DNA repair in Deinococcus radiodurans, we examined proteome changes in a wild-type strain following gamma-irradiation using two-dimensional polyacrylamide gel electrophoresis and Silver-staining. The expression levels of 26 protein spots showed significant changes under radiation stress. Of these spots, 21 were identified with peptide mass fingerprinting using matrix-assisted laser desorption/ionization-time of flight mass spectrometry after tryptic in-gel digestion. These proteins exhibited various cellular functions, including (i) translation; (ii) transcription; (iii) signal transduction; (iv) post-translational modification, protein turnover, chaperones; (v) carbohydrate transport and metabolism; (vi) energy production and conversion; (vii) nucleotide transport and metabolism; (viii) inorganic ion transport and metabolism; (ix) DNA replication, recombination and repair; and (x) yet unknown. Most of the proteins have not previously been reported to be relevant to radioresistance.
PROTEOMICS 02/2005; 5(1):138-43. · 4.51 Impact Factor
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ABSTRACT: The protein DRA0074 is suggested to be another LexA in Deinococcus radiodurans, having similar motifs and RecA-mediated cleavage activity to D. radiodurans LexA (dra0344). However, its function has not been studied. We disrupted the gene dra0074 and measured its effect on RecA induction using fusion translation, immunoblot, and proteomic analysis. Results showed that the product of gene dra0074 is not involved in RecA induction, but is a regulator of other metabolisms in D. radiodurans.
Journal of Biochemistry 01/2005; 136(6):787-93. · 2.37 Impact Factor
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ABSTRACT: Free radical scavenging effects of the cellular protein extracts from two strains of Deinococcus radiodurans and Escherichia coli against O2-, H2O2 and *OH were investigated by chemiluminescence (CL) methods. The cellular protein extracts of D. radiodurans R1 and KD8301 showed higher scavenging effects on O2- than that of E. coli. D. radiodurans R1 and KD8301 also strongly scavenged H2O2 with an EC50 (50% effective concentration) of 0.12 and 0.2 mg/mL, respectively, compared to that of E. coli (EC50 = 3.56 mg/mL). The two strains of D. radiodurans were effective in scavenging *OH generated by the Fenton reaction, with EC50 of 0.059 and 0.1 mg/mL, respectively, compared to that of E. coli (EC50 > 1 mg/mL). Results from the chemiluminescence assay of *OH-induced DNA damage and the plasmid pUC18 DNA double-strand break (DSB) model in vitro showed that D. radiodurans had remarkably inhibitory effect on the *OH-induced oxidative damage of DNA. The scavenging effects of D. radiodurans on reactive oxygen species (ROS) played an important role in the response to oxidation stress and preventing against DNA oxidative damage, and may be attributed to intracellular scavenging proteins, including superoxide dismutase (SOD) and catalase.
Luminescence 19(2):78-84. · 1.73 Impact Factor
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ABSTRACT: In InCl3–NaBH4–MeCN system, terminal aryl alkynes could couple with aryl iodides and bromides to give disubstituted alkenes via hydroindation of CC. In the similar way, (E)-alkenylsilanes were synthesized via reduction of alkynylsilanes in tetrahydrofuran (THF) in high yields. The processes showed high regio- and stereoselectivity.Graphical abstract
Tetrahedron. 62(33):7712-7717.
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ABSTRACT: InCl3-catalyzed reduction of anthrones and anthraquinones was investigated under different conditions. A new synthetic method for anthracenes in aqueous media under mild conditions is described.Graphical abstract
Tetrahedron. 63(23):5071-5075.
