Y Aragane

Publications (36)153.33 Total impact

• Source

  Available from: jiaci.org

  Article: Inhibition of cytokine-induced expression of T-cell cytokines by antihistamines.

  M S Ashenager, T Grgela, Y Aragane, A Kawada
  [show abstract] [hide abstract]
  ABSTRACT: To investigate immunomodulatory properties of 4 antihistamines available in Japan. Isolated peripheral blood T cells from healthy volunteers were preincubated with cetirizine, loratadine, olopatadine, or fexofenadine for 30 minutes and then stimulated with interleukin (IL)-1 2 or IL-4 to skew immune response towards type 1 or type 2 helper T cells. RNA was extracted 6 hours later and semiquantitative reverse transcription polymerase chain reaction (RT-PCR) was performed using primers for IL-5 and interferon (IFN) gamma. Supernatants were collected 24 hours after stimulation, and cytokine production was quantified by enzyme-linked immunosorbent assay (ELISA). RT-PCR revealed that IL-12-induced expression of IFN-gamma was partially suppressed by loratadine and fexofenadine and that all 4 agents tested inhibited IL-4-induced expression of IL-5. ELISA demonstrated that IL-12-induced IFN-gamma production was significantly suppressed by cetirizine and fexofenadine and IL-4-induced IL-5 production was downregulated by three agents with the exception of cetirizine. This study demonstrates that antihistamines have varying immunomodulatory properties, suggesting treatment choice for atopic dermatitis can be directed by disease signs and symptoms.
  Journal of investigational allergology & clinical immunology: official organ of the International Association of Asthmology (INTERASMA) and Sociedad Latinoamericana de Alergia e Inmunología 02/2007; 17(1):20-6. · 2.27 Impact Factor
• Article: Contact urticaria due to ketoprofen.

  T Suzuki, A Kawada, Y Hashimoto, R Isogai, Y Aragane, T Tezuka
  Contact Dermatitis 06/2003; 48(5):284-5. · 3.51 Impact Factor
• Article: A case of naevus lipomatosus cutaneus superficialis of the scalp associated with pedunculated basal cell carcinoma.

  A Maeda, Y Aragane, K Ueno, F Yamazaki, A Kawada, T Tezuka
  British Journal of Dermatology 06/2003; 148(5):1084-6. · 3.67 Impact Factor
• Article: Hydroxyurea-induced foot ulcer successfully treated with a topical basic fibroblast growth factor product.

  Y Aragane, T Okamoto, A Yajima, R Isogai, A Kawada, T Tezuka
  British Journal of Dermatology 04/2003; 148(3):599-600. · 3.67 Impact Factor
• Article: Nail scabies as an initial lesion of ordinary scabies.

  R Isogai, A Kawada, Y Aragane, T Tezuka
  British Journal of Dermatology 10/2002; 147(3):603. · 3.67 Impact Factor
• Article: Immunohistochemical detection for nuclear beta-catenin in sporadic basal cell carcinoma.

  F Yamazaki, Y Aragane, A Kawada, T Tezuka
  [show abstract] [hide abstract]
  ABSTRACT: Despite the increasing incidence of basal cell carcinoma (BCC), its pathogenesis has remained largely unknown. Recently, it was reported that genes involved in tissue morphogenesis, such as sonic hedgehog or patched, were found to be mutated in BCC, suggesting the involvement of those molecules in the pathogenesis of this tumour. Furthermore, there is evidence that the Wnt-mediated signalling pathway may be one of the downstream targets of sonic hedgehog-mediated signalling, which has led us to focus on molecular events on the Wnt pathway in BCC. Among the signal transducers involved in the Wnt pathway, it is clear that beta-catenin plays a pivotal role in the promotion of morphogenesis and cell growth. In respect to this, it has been reported that, in particular circumstances, as in colorectal cancers, beta-catenin migrates to the nuclei, where it exerts an ability to activate the transcription of various genes. To investigate the cellular distribution of beta-catenin in skin tumours, in particular, in BCC. Twenty skin biopsy specimens derived from BCC, 10 from inflammatory skin diseases and five from squamous cell carcinomas were immunostained with an antibody directed against beta-catenin. Fourteen of the 20 BCC samples tested showed nuclear localization of beta-catenin, while none of the other samples gave rise to positive nuclear staining. Nuclear localization of beta-catenin is a characteristic feature of BCC; this suggests its tumorigenic role in this tumour. This gives us a further insight into the molecular pathogenesis of BCC.
  British Journal of Dermatology 12/2001; 145(5):771-7. · 3.67 Impact Factor
• Article: Allergic contact cheilitis due to glyceryl monoisostearate monomyristate in a lipstick.

  M Asai, A Kawada, Y Aragane, T Tezuka
  Contact Dermatitis 10/2001; 45(3):173. · 3.51 Impact Factor
• Article: A case of acral lentginous melanoma: the correlation between CD95L expression on melanoma cells and apoptosis of tumor infiltrating lymphocytes.

  A Maeda, Y Aragane, A Kawada, R Isogai, T Orita, T Tezuka
  [show abstract] [hide abstract]
  ABSTRACT: There is an increasing amount of evidence that melanoma cells express the ligand for CD95 (CD95L), a potent inducer of apoptosis which contributes to creating the immune privileged circumstances of tumor sites. However, it still remains to be demonstrated whether the capacity of melanoma cells to express CD95L is acquired during the progression. We addressed this question with a case of acral lentiginous melanoma by employing immunostaining using an antibody directed against CD95L as well as by in situ TUNEL staining. H&E-staining of tumor specimens revealed that there were two different growth patterns. The central part of the tumor showed a deeper invasion into the dermis (Breslow thickness >4 -mm). The horizontally growing edge of the tumor proliferated more superficially (Breslow thickness<3-mm). Relatively fewer lymphocytes were observed around the melanoma nests in central areas, which expressed detectable amounts of CD95L. In contrast, more lymphocytes were observed among the melanoma cells in the peripheral lesion, where CD95L was not detected. To evaluate the relevance of the CD95L expression, in situ TUNEL staining was performed. This indicated a significant correlation of lymphocyte apoptosis with CD95L expression on melanoma cells. Together the data suggest that expression of CD95L is turned on depending on the level of melanoma, and that it may tribute to creating immune privileged circumstances by initiating apoptosis of tumor filrating lymphocytes.
  The Journal of Dermatology 09/2001; 28(9):499-504. · 1.49 Impact Factor
• Article: Simultaneous photocontact sensitivity to ketoprofen and oxybenzone.

  A Kawada, Y Aragane, M Asai, T Tezuka
  Contact Dermatitis 07/2001; 44(6):370. · 3.51 Impact Factor
• Article: Drug eruption induced by cefcapene pivoxil hydrochloride.

  A Kawada, Y Aragane, A Maeda, M Asai, H Shiraishi, T Tezuka
  Contact Dermatitis 04/2001; 44(3):197. · 3.51 Impact Factor
• Article: Pyoderma gangrenosum associated with biphenotypic acute leukemia.

  M Asai, Y Aragane, A Kawada, T Shimada, A Kanamaru, H Yamada, T Tezukam
  [show abstract] [hide abstract]
  ABSTRACT: Pyoderma gangrenosum is a neutrophilic dermatosis that may be associated with myeloid malignancies. Less information is available about the association of pyoderma gangrenosum with lymphoid malignancies. We present, to our knowledge, the first case of pyoderma gangrenosum associated with biphenotypic acute leukemia wherein the malignant cells show a phenotype specific for myelogenic and lymphocytic leukemia. Histopathologic examination revealed rather nonspecific features without involvement of leukemic cells in the skin lesions. Treatment with systemic steroids was followed by characteristically rapid healing of the skin lesion.
  Journal of the American Academy of Dermatology 04/2001; 44(3):530-1. · 3.99 Impact Factor
• Article: Fixed drug eruption due to scopolia extract.

  A Kawada, Y Aragane, H Shiraishi, T Tezuka
  Contact Dermatitis 03/2001; 44(2):101. · 3.51 Impact Factor
• Article: Detection of HTLV-I proviral DNA in sarcoidosis.

  A Yajima, A Kawada, Y Aragane, T Tezuka
  [show abstract] [hide abstract]
  ABSTRACT: 'Sarcoidosis-lymphoma syndrome' is known as an association of sarcoidosis with malignant lymphoma. We report a 56-year-old woman with systemic sarcoidosis who was seropositive for antibody against human T cell lymphoma/leukemia virus type I (HTLV-I). This patient showed integration of HTLV-I proviral DNA within cutaneous sarcoid nodules, but not in peripheral blood mononuclear cells. Neither atypical lymphocytes nor a T cell receptor beta1 gene rearrangement were observed in peripheral blood mononuclear cells or in cutaneous nodules, indicating that the patient did not have a smouldering type of adult T cell lymphoma/leukemia. Detection of integration of HTLV-I proviral DNA in cutaneous sarcoid nodules could suggest that the sarcoid nodules might have been generated as a protective response to chronic stimuli of HTLV-I.
  Dermatology 02/2001; 203(1):53-6. · 2.05 Impact Factor
• Article: Inhibition of growth of melanoma cells by CD95 (Fas/APO-1) gene transfer in vivo.

  Y Aragane, A Maeda, C Y Cui, T Tezuka, Y Kaneda, T Schwarz
  [show abstract] [hide abstract]
  ABSTRACT: Interaction of CD95 ligand with its cognate receptor CD95 induces apoptotic cell death. Alterations in this pathway within tumor cells can result in escape from apoptosis and from immune surveillance. Melanoma cells recently were found to escape an immune attack via high expression of CD95 ligand, thereby inducing apoptosis of activated T lymphocytes. When screening four human melanoma cell lines for expression of CD95 and CD95 ligand, respectively, an inverse correlation was found, i.e., cells expressing high levels for CD95 ligand (CD95L(high)) were almost negative for CD95 and vice versa. Since coexpression of CD95 and CD95 ligand may lead to apoptosis by autocrine suicide or fratricide, it was tested whether overexpression of CD95 in CD95L(high) melanoma cells results in apoptotic cell death. Upon transfection with a cytomegalovirus-promoter-driven expression vector encoding the CD95 gene, CD95L(high) melanoma cells underwent apoptosis at a much higher level than CD95L(low) melanoma cells. Apoptosis appeared to be due to the activation of CD95 as cell death was inhibited by cotransfection with a dominant negative mutant for the CD95 signaling protein, Fas-associated protein with death domain. Tumor progression of CD95L(high) melanoma cells transplanted into nude mice was significantly reduced when recipient animals were injected with liposomes containing the CD95 expression vector. As demonstrated by immunohistochemistry and TUNEL staining, in vivo transfected tumor cells expressed CD95 and underwent apoptotic cell death. Hence, this study indicates that delivery of the CD95 gene inhibits tumor growth in vivo and thus might be a therapeutic strategy to treat tumor cells that express high levels of CD95 ligand. J Invest Dermatol 115:1008-1014 2000
  Journal of Investigative Dermatology 01/2001; 115(6):1008-14. · 6.31 Impact Factor
• Article: Fixed drug eruption induced by tosufloxacin tosilate.

  Y Sangen, A Kawada, M Asai, Y Aragane, T Yudate, T Tezuka
  Contact Dermatitis 06/2000; 42(5):285. · 3.51 Impact Factor
• Article: Tinea barbae due to Trichophyton rubrum with possible involvement of autoinoculation.

  A Kawada, Y Aragane, A Maeda, T Yudate, T Tezuka, M Hiruma
  British Journal of Dermatology 06/2000; 142(5):1064-5. · 3.67 Impact Factor
• Article: Contact dermatitis due to flurbiprofen.

  A Kawada, Y Aragane, A Maeda, T Yudate, T Tezuka
  Contact Dermatitis 04/2000; 42(3):167-8. · 3.51 Impact Factor
• Article: Drug eruption due to tribenoside.

  H Endo, A Kawada, T Yudate, Y Aragane, H Yamada, T Tezuka
  Contact Dermatitis 11/1999; 41(4):223. · 3.51 Impact Factor
• Article: Bikunin, a serine protease inhibitor, is present on the cell boundary of epidermis.

  C Y Cui, Y Aragane, A Maeda, Y L Piao, M Takahashi, L H Kim, T Tezuka
  [show abstract] [hide abstract]
  ABSTRACT: Bikunin, which is an inhibitor of serine proteases, is widely distributed in human tissues, including liver, kidney, and mucous membranes of the stomach and colon. The aim of this study was to clarify whether bikunin is expressed in human epidermis and its appendages. Immunoblot analysis using a specific polyclonal antibody to bikunin revealed that a single 43 kDa protein is present in the cell lysate from the human keratinocyte cell line HaCaT. Immunohistochemically, dotted reaction products stained with anti-bikunin antibody were localized on the cell boundary in both basal and spinous cell layers, except on the cell boundary of the basal cells facing the basal membrane. There were no reaction products in the granular-horny cell layers. Reaction products stained with anti-bikunin antibody were also observed on the hair bulb cells and eccrine sweat gland cells, but not on apocrine sweat glands. Also, reaction products were observed on the luminal surface of the renal proximal tubules and in the cytoplasm of these cells. In immunoelectron microscopy, gold particles were observed on the cell membranes close to the desmosomal structures. Reverse transcription-polymerase chain reaction and northern blot analyses showed that mRNA specific for bikunin was expressed in HaCaT cells and human epidermal keratinocytes obtained from suction blisters, and was contained in a commercially available human keratinocyte cDNA preparation. These findings indicate that bikunin is expressed in keratinocytes and may play an important part in regulating keratinocytes in either mitosis or inflammation.
  Journal of Investigative Dermatology 09/1999; 113(2):182-8. · 6.31 Impact Factor
• Article: Expression of CD95 ligand in melanocytic lesions as a diagnostic marker.

  A Maeda, Y Aragane, T Tezuka
  [show abstract] [hide abstract]
  ABSTRACT: CD95 ligand (CD95L) potently induces apoptosis by activating CD95 on target cells. It has recently been reported that melanoma cells in vivo express a significant amount of CD95L, thereby being immediately able to kill CD95-bearing immunocompetent cells specific for cancer antigens, which infiltrate the lesions. In this study, we employed immunohistochemistry using an antibody directed against CD95L to investigate at which stage the melanoma CD95L expression is turned on. Skin biopsies of 49 lesions from 46 patients were assessed. These included benign and dysplastic naevi, melanoma in situ, stage I melanomas (Clark's level 2 or 3), advance-phase melanomas (Clark's level 4 or 5) and lymph node metastases. CD95L was expressed in all of the advance-phase melanomas as well as lymph node metastases of cutaneous origin, whereas neither melanoma in situ, benign naevi nor dysplastic naevi reacted positively with the antibody. To investigate a link between positivity and tumour size, the data were analysed on the basis of Breslow thickness, and indicated that expression was observed only when tumours were thicker than 0.75 mm. We next compared expression of CD95L and HMB-45. CD95L was positive only in melanomas in a more advanced phase than stage I, whereas HMB-45 was not only expressed in melanoma cells but also in benign pigmented naevi. This indicated the advantage of CD95L staining to diagnose melanoma. The present study indicates the significant correlation between tumorigenicity and expression of CD95L, and thereby raises the possibility that CD95L may be a useful diagnostic marker for malignant melanomas.
  British Journal of Dermatology 09/1998; 139(2):198-206. · 3.67 Impact Factor