Svjetlana Terzić

Croatian Veterinary Institute, Zagrabia, Grad Zagreb, Croatia

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Publications (60)84.24 Total impact

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    ABSTRACT: The aim of the study was to compare the accumulation of β-adrenergic agonist residues clenbuterol (CLB) and salbutamol (SAL) in internal tissues, non-pigmented eyes and hair of laboratory animals repeatedly administered with CLB and SAL during 7 days. Experimental albino guinea pigs (n = 20) were treated with CLB (n = 10) and SAL (n = 10) in anabolic doses of 0.25 and 2.5 mg/kg, whereas the control animal group (n = 10) was left untreated. Methodology validation showed that the ELISA assay to be suitable for β-agonists' semiquantitative determination. The results revealed a significantly higher (P < 0.05) accumulation potential of CLB in comparison with SAL in all investigated tissues. Despite of their lack of pigmentation and the applied dose, the highest residual CLB concentrations were determined in the eyes of the studied animals, followed by their hair, liver, lungs, kidney, heart and adipose and muscle tissue, whereas residual SAL concentrations found in the eyes and hair of the administered animals did not significantly differ (P > 0.05) from those obtained in their internal tissues.
    Journal of analytical toxicology 07/2014; 38(9). DOI:10.1093/jat/bku081 · 2.63 Impact Factor
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    ABSTRACT: Tick-borne encephalitis (TBE) is a growing public health concern in central and northern European countries. Even though TBE is a notifiable disease in Croatia, there is a significant lack of information in regard to vector tick identification, distribution as well as TBE virus prevalence in ticks or animals. The aim of our study was to identify and to investigate the viral prevalence of TBE virus in ticks removed from red fox (Vulpes vulpes) carcasses hunted in endemic areas in northern Croatia and to gain a better insight in the role of wild ungulates, especially red deer (Cervus elaphus) in the maintenance of the TBE virus in the natural cycle. We identified 5 tick species (Ixodes ricinus, Ixodes hexagonus, Haemaphysalis punctata, Dermacentor reticulatus, Rhipicephalus sanguineus) removed from 40 red foxes. However, TBE virus was isolated only from adult I. ricinus and I. hexagonus ticks showing a viral prevalence (1.6%) similar to or higher than reported in endemic areas of other European countries. Furthermore, 2 positive spleen samples from 182 red deer (1.1%) were found. Croatian TBE virus isolates were genetically analyzed, and they were shown to be closely related, all belonging to the European TBE virus subgroup. However, on the basis of nucleotide and amino acid sequence analysis, 2 clusters were identified. Our results show that further investigation is needed to understand the clustering of isolates and to identify the most common TBE virus reservoir hosts in Croatia. Sentinel surveys based on wild animal species would give a better insight in defining TBE virus-endemic and possible risk areas in Croatia.
    Ticks and Tick-borne Diseases 09/2013; 5(1). DOI:10.1016/j.ttbdis.2012.11.016 · 2.88 Impact Factor
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    J Pleadin · A Vulic · N Persi · S Terzic · M Andrisic · I Zarkovic
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    ABSTRACT: The aim of the study was to evaluate the adequacy of enzyme-linked immunosorbent assay (ELISA) in the post-exposure determination of the β-agonists clenbuterol and salbutamol in animal plasma and serum. Experimental guinea pigs (n = 20) were treated with two doses (0.25 and 2.5 mg/kg) of clenbuterol (n = 10) and salbutamol (n = 10) for seven days, whereas the control animal group (n = 10) was left untreated. Validation of the applied method yielded acceptable recovery (mean > 70%) and repeatability rates, showing ELISA to be applicable for the semi-quantitative determination of both analytes in both matrices, preferably in plasma. In both matrices, clenbuterol concentrations were proven to be significantly (14-fold) higher than those of salbutamol. Concentrations of both analytes were higher in plasma than in serum. The application of a 10-fold higher clenbuterol and salbutamol dose (2.5 mg/kg) resulted in concentrations 3- to 4-fold higher for clenbuterol and 2- to 3-fold higher for salbutamol, indicating a different release rate of these two β-agonists.
    Journal of analytical toxicology 03/2013; 37(4). DOI:10.1093/jat/bkt017 · 2.63 Impact Factor
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    ABSTRACT: The aim of the present study was to assess the accumulation of ractopamine residues in the hair and ocular tissues of guinea pigs during repeated ractopamine administration and after treatment. The experiment was conducted in 38 guinea pigs (30 treated and eight controls). Treated animals were orally administered ractopamine hydrochloride in a dose of 3.5 mg/kg body mass per day using probes for seven consecutive days. Ractopamine concentration was determined in hair during the treatment (Days 1, 3 and 7) with ractopamine hydrochloride and in ocular tissues and hair on defined days after exposure (Days 1, 10, 20 and 30). Residues were present in hair in high concentrations as early as Day 3 (86.15 ± 87.71 ng/g) and Day 7 (85.25 ± 56.97 ng/g). After exposure, residues were found to persist, having depleted from 68.06 ± 30.54 ng/g on Day 1 to 8.01 ± 2.22 ng/g on Day 30, with a significantly higher concentration in hair in contrast to low residue levels in ocular tissues (1.20-0.34 ng/g). The results of the study pointed to high ractopamine accumulation, even in non-pigmented hair, suggesting hair to be used as a matrix in the control of ractopamine abuse in farm animals because of its many advantages over ocular tissues.
    Journal of analytical toxicology 01/2013; 37(2). DOI:10.1093/jat/bks092 · 2.63 Impact Factor
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    ABSTRACT: The aim of the study was to compare residue depletion of ractopamine HCl as a β-adrenergic agonist that promotes muscle growth of animals, from internal tissues on days after its repeat administration to animals. The experiment was carried out in 38 albino guinea pigs. Treated animals (n = 30) were orally administered ractopamine HCl in a dose of 3.5 mg/kg body mass per day for 7 consecutive days. On days 1, 10, 20 and 30 of drug discontinuation, animals were randomly sacrificed and the liver, kidney, lung, heart, muscle, spleen and fat samples were collected. In all matrices, ractopamine concentration was determined using validated enzyme-linked immunosorbent assay (ELISA) as a quantitative screening method. The highest ractopamine concentration was recorded on day 1 in the lungs (55.80 ± 15.62 μg/kg), followed by the kidney (21.85 ± 3.91 μg/kg), spleen (12.59 ± 1.95 μg/kg), fat (10.17 ± 5.02 μg/kg), heart (9.73 ± 0.22 μg/kg), liver (5.58 ± 2.09 μg/kg), and lowest in muscle (2.21 ± 1.02 μg/kg). Ractopamine residues were detected in the lungs in the period of 30 days after withdrawal in significantly higher concentrations in comparison to other investigated matrices, suggesting that depletion of ractopamine from the lungs occurs at a much slower rate than its depletion from other internal tissues.
    Immunopharmacology and Immunotoxicology 07/2012; 35(1). DOI:10.3109/08923973.2012.702115 · 1.11 Impact Factor
  • 48th Congress of the European-Societies-of-Toxicology (EUROTOX); 06/2012
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    ABSTRACT: The objective of this study was to investigate the effect of ochratoxin A (OTA) on serum biochemical parameters of pigs during subchronic treatment with 300 μg OTA/kg of feed for 30 days. OTA treatment resulted in significantly higher (p < 0.05) serum levels of creatinine, urea, potassium and alkaline phosphatase, and significantly lower levels of glucose and total protein. These changes in serum biochemical parameters in treated pigs were indicative of impaired liver and kidney function caused by OTA exposure.
    Bulletin of Environmental Contamination and Toxicology 03/2012; 88(6):1043-7. DOI:10.1007/s00128-012-0615-x · 1.26 Impact Factor
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    Veterinary Archives 03/2012; 82(2). · 0.30 Impact Factor
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    ABSTRACT: Premixes for medicated feedstuffs are considered to be veterinary medicinal products (VMPs) prepared in advance with a view to the subsequent manufacture of medicated feedstuffs. Medicated feedstuffs should be prepared only from market authorized premixes and premixes for medicated feedstuffs can be used only as prescribed medicines. Apart from active substances, premixes contain carriers which have a role in the homogenization of medicated feedstuffs. Wheat bran as a carrier may be a source of different, potentially harmful or toxic substances e.g. afl atoxin. In our study, 15 different batches of premixes with a wheat bran component were tested for afl atoxin B1 (AFB1) by means of an enzyme-linked immunosorbent assay (ELISA). The samples tested showed contamination with AFB1 ranging from 1.5 to 35 ng/g (mean = 18.79 ng/g). However, no correlation between AFB1 levels and the month of collection or season of production was observed. Considering the composition of the premix, the only possible source of contamination with AFB1 was wheat bran. Contamination probably occurred before the production of the premix, probably in the fi eld or during storage. Concentrations of active substances and citric acid (a neutralising agent for mycotoxins) were in accordance with the producer’s declaration. Our discovery of AFB1 in the tested premixes was in contravention of the defi nition and primary role of veterinary medicinal products. In this study, we would like to highlight the need for monitoring raw materials of biological origin for premix production. Even though carriers are not pharmacologically active substances, an effi cient method for controlling potential contaminants such as mycotoxins or their toxic components should be proposed, with the aim of protecting animal health, consumers, employees and end-users in the production of veterinary medicinal products.
    Veterinarski Arhiv 01/2012; 82(2):155-166. · 0.30 Impact Factor
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    IPVS 2012 KOREA; 01/2012
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    Svjetlana Terzic · Besi Roic · Lorena Jemersic · T. Florijancic
    Acta veterinaria 01/2012; 62(2-3):289-296. DOI:10.2298/AVB1203289T · 0.13 Impact Factor
  • Nina Bilandzic · Svjetlana Terzic · B. Simic
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    ABSTRACT: The objective of this paper was to determine the effect of levamisole (LEV) on immuno globulin concentration in the serum of boars exposed to 3-day stress induced by exogenous ACTH. Boars were assigned to 4 groups (n=7). The first group received LEV for 3 days (2.5 mg/kg BW), the second group received ACTH (10 mu g/kg BW) for 3 days and the third group received LEV for 3 consecutive days and ACTH for the following 3 days (2.5 mg/kg BW; ACTH 10 mu g/kg BW). The control group received saline solution during the 6 days. Concentrations of cortisol, total protein, globulin, albumin and immuno globulin (IgG, IgA and IgM) were determined during treatments and on the 16th day post-administration. Cortisol concentration was increased in both ACTH treated groups during all three days of administration and the day after the last ACTH treatment (p < 0.05). ACTH increased total protein levels during the stress period and over the next 16 days (p < 0.05). However, in the LEV+ACTH group total protein levels were elevated only on day 1 and 2 of ACTH injection (p < 0.05) and after the end of treatment on day 11 and 22 (p < 0.05). LEV stimulated the increase of protein concentrations compared to control values after LEV treatment, on days 5, 14, 18 and 22 (p < 0.05). Serum albumins were not affected by LEV or ACTH treatment. Globulin concentrations were increased throughout and on the 16th day after administration of ACTH in the ACTH and LEV+ACTH groups (p < 0.05). Globulin concentrations did not differ between LEV and control groups of boars. ACTH treatment elevated serum IgG concentration during the stress period (p < 0.05) and over the next 16 days (p < 0.05). However, in the LEV+ACTH group of boars, IgG levels were elevated on days 1 and 3 after ACTH injection (p < 0.05) and days 1 and 5 in the post-treatment period (p < 0.05). LEV had no impact on IgG levels compared to the control boars. However, increased IgA concentrations in boars treated with LEV were determined on day 2 (p < 0.05) and day 11 (p < 0.05) after administration of LEV compared to animals in the LEV+ACTH group. These results show that LEV application may protect boars from the negative influence of stress and provoke improved non-specific immunity.
    Acta veterinaria 01/2012; 62(5-6):495-509. DOI:10.2298/AVB1206495B · 0.13 Impact Factor
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    Congress of the European Societies of Toxicology; 01/2012
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    ABSTRACT: We determined prevalence of antibody to selected viral pathogens important for domestic pigs and livestock in 556 wild boar (Sus scrofa) sera collected during 2005-06 and 2009-10 in four counties in Croatia. These counties account for an important part of the Croatian commercial pig production and have a high density of wild boars. Samples were tested for antibodies to porcine parvovirus (PPV), Aujeszky's disease virus (ADV), porcine circovirus type 2 (PCV2), swine influenza virus, porcine respiratory and reproductive syndrome virus (PRRSV), porcine respiratory coronavirus (PRCV), transmissible gastroenteritis virus, and swine vesicular disease virus (SVDV). Antibodies to all of the infectious pathogens except SVDV were detected. There was a statistically significant difference in prevalence between the two periods for PPV, ADV, PCV2, PRRSV, and PRCV, with a higher prevalence of PPV and ADV in the 2009-10 period (P<0.05). During the same period, the prevalence of PCV2, PRRSV, and PRCV was lower (P<0.05). Our results provide information on the current disease exposure and health status of wild boars in Croatia and suggest that wild boars may act as a reservoir for several pathogens and a source of infection for domestic pigs and other livestock as well as humans, especially for ADV.
    Journal of wildlife diseases 01/2012; 48(1):131-7. DOI:10.7589/0090-3558-48.1.131 · 1.31 Impact Factor
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    ABSTRACT: Premixes for medicated feedstuffs are considered to be veterinary medicinal products (VMPs) prepared in advance with a view to the subsequent manufacture of medicated feedstuffs. Medicated feedstuffs should be prepared only from market authorized premixes and premixes for medicated feedstuffs can be used only as prescribed medicines. Apart from active substances, premixes contain carriers which have a role in the homogenization of medicated feedstuffs. Wheat bran as a carrier may be a source of different, potentially harmful or toxic substances e.g. afl atoxin. In our study, 15 different batches of premixes with a wheat bran component were tested for afl atoxin B1 (AFB1) by means of an enzyme-linked immunosorbent assay (ELISA). The samples tested showed contamination with AFB1 ranging from 1.5 to 35 ng/g (mean = 18.79 ng/g). However, no correlation between AFB1 levels and the month of collection or season of production was observed. Considering the composition of the premix, the only possible source of contamination with AFB1 was wheat bran. Contamination probably occurred before the production of the premix, probably in the fi eld or during storage. Concentrations of active substances and citric acid (a neutralising agent for mycotoxins) were in accordance with the producer’s declaration. Our discovery of AFB1 in the tested premixes was in contravention of the defi nition and primary role of veterinary medicinal products. In this study, we would like to highlight the need for monitoring raw materials of biological origin for premix production. Even though carriers are not pharmacologically active substances, an effi cient method for controlling potential contaminants such as mycotoxins or their toxic components should be proposed, with the aim of protecting animal health, consumers, employees and end-users in the production of veterinary medicinal products.
    Veterinarski Arhiv 01/2012; 82(2):155-166. · 0.30 Impact Factor
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    S Terzić · L Jemeršić · B Roić · K Šandor
    IPVS 2012 KOREA; 01/2012
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    ABSTRACT: The method for virus titer determination of avian infectious bursal disease (IBD) live vaccine, developed long before regulatory validation guidelines is a cell culture based biological assay intended for use in vaccine release testing. The aim of our study was to perform a validation, based on fit-for-purpose principle, of an old 50% tissue culture infectious dose (TCID(50)) method according to Guidelines of the International Cooperation on Harmonization of Technical Requirements for Registration of Veterinary Medicinal Products (VICH). This paper addresses challenges and discusses some key aspects that should be considered when validating biological methods. A different statistical approach and non-parametric statistics was introduced in validation protocol in order to derive useful information from experimental data. This approach is applicable for a wide range of methods. In conclusion, the previous virus titration method had showed to be precise, accurate, linear, robust and in accordance with current regulatory standards, which indicates that there is no need for additional re-development or upgrades of the method for its suitability for intended use.
    Biologicals 01/2012; 40(1):41-8. DOI:10.1016/j.biologicals.2011.09.008 · 1.41 Impact Factor
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    ABSTRACT: High performance liquid chromatography was validated and applied for the determination of amoxicillin in veterinary medicinal products. Validation was conducted on three batches of three diff erent veterinary medicinal products from various manufacturers. Selectivity, linearity and repeatability met the acceptance criteria for the validation of the analytical method. The UV-DAD spectra of the amoxicillin trihidrate peak in the chromatogram of the sample were from 99.98% to 99.99%. The amoxicillin assay in the samples ranged from 95.10% to 103.95% of the declared content, which complied with the requirements of the manufacturer’s specifi cations. The measurement uncertainty of the method is estimated by the contribution of the standard, sample preparation, linearity and repeatability. The measurement uncertainty of the method was 0.024 mg/mL at the 95% confi dence level.
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    I Mikac · Z Fiket · S Terzić · J Barešić · N Mikac · M Ahel
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    ABSTRACT: The anthropogenic impact on the pristine karst lakes was investigated using combination of specific parameters, including multielemental analysis of major inorganic constituents (Al, K, Fe) and trace metals (Li, Ag, Cd, Sn, Pb, Bi, Cr, Co, Ni, Cu, Zn and Sb), polycyclic aromatic hydrocarbons (PAHs) and anionic surfactants of linear alkylbenzene sulfonate (LAS) type. The study was performed in the Plitvice Lakes National Park, situated in a sparsely populated area of the northwestern Dinarides, central Croatia. Dated cores of recent sediments from the two biggest lakes, Lake Prosce and Lake Kozjak, were analysed for the selected contaminants using highly specific methods, involving inductively coupled plasma mass spectrometry (ICP/MS), gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/tandem mass spectrometry (LC/MS/MS). The concentration of inorganic constituents reflected primarily the geological background of the area as well as geomorphological and geochemical characteristics of the Plitvice Lakes. Due to the higher terrigenous input, the concentration of all elements was significantly higher in the Lake Prosce. The concentration of toxic metals was relatively low in both lakes, except for Cd (>1 mg kg(-1)) and Pb (up to 40 mg kg(-1)). The vertical profiles of these metals suggested that elevated concentrations of Cd were of natural origin, derived from the erosion of the Jurassic dolomite bedrock, while Pb was predominately of recent anthropogenic origin. A similar distribution pattern, suggesting the same prevailing mechanism of input, was observed for pyrolytic PAHs. The characteristic diagnostic PAH ratios revealed that higher PAHs prevailingly originated from the combustion of biomass and fossil fuels. LAS, which represent highly specific indicators of untreated wastewaters, were found in rather high concentrations in the recent sediment layers (up to 4.7 mg kg(-1)), suggesting that contaminated household and hotel wastewaters reach the Lakes, very probably by leaking through the porous karst rocks.
    Chemosphere 04/2011; 84(8):1140-9. DOI:10.1016/j.chemosphere.2011.04.027 · 3.50 Impact Factor
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    ABSTRACT: Picoplankton distribution at the boundary zone of the southern Adriatic in May 2009 on a 75 km long shelf-continental slope transect was assessed by combining epifluorescence microscopy, flow cytometry and high-performance liquid chromatography data with hydrographic observations. The picoplankton distribution was greatly influenced by the hydrographic conditions prevailing in the southern Adriatic because of the influence of the Levantine Intermediate Water (LIW) and East Adriatic Current (EAC) forcing. Heterotrophic bacteria numerically dominated the picoplankton community through the entire transect with no significant accumulation. By contrast, picophytoplankton accumulated in the 50–75 m layer, forming a pronounced deep chlorophyll maximum. Synechococcus dominated the photosynthetic picoplankton, whereas picoeukaryotes were the least abundant. The intrusion of warm LIW observed in the layer between 100 and 350 m was followed by Prochlorococcus and Synechococcus peaks (10 × 103 cells mL−1 and 90 × 103 cells mL−1, respectively), as well as by the appearance of two Synechococcus ecotypes. Most picoeukaryotes were observed at the offshore stations, where geostrophic current calculation revealed the strongest EAC influence. A strong EAC spread over the central and eastern basin created a barrier for Prochlorococcus, whereas the picoeukaryote maxima coincided with the core of the EAC, suggesting its persistence to hydrological instabilities.Research highlights► We examined the influence of hydrographic conditions on picoplankton distribution. ► Picophytoplankton accumulated from 50 to 75 m, forming a deep chlorophyll maximum. ► LIW intrusion was followed by the appearance of two Synechococcus ecotypes. ► The East Adriatic Current (EAC) acted like a barrier for Prochlorococcus. ► The picoeukaryotes maxima coincided with the core of the EAC.
    Estuarine Coastal and Shelf Science 03/2011; 91(4):519-525. DOI:10.1016/j.ecss.2010.12.012 · 2.25 Impact Factor

Publication Stats

612 Citations
84.24 Total Impact Points

Institutions

  • 2001–2014
    • Croatian Veterinary Institute
      • • Laboratory for Analytical Chemistry and Residue
      • • Department of Virology
      Zagrabia, Grad Zagreb, Croatia
  • 2008–2011
    • Ruder Boskovic Institute
      Zagrabia, Grad Zagreb, Croatia
  • 1993–2011
    • Ruđer Bošković Institute
      • Division for Marine and Environmental Research
      Zagreb, Grad Zagreb, Croatia