Gao-Xue Wang

Northwest University, Northwest Harborcreek, Pennsylvania, United States

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Publications (40)103.96 Total impact

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    ABSTRACT: Trifloxystrobin (TFS) is the widely used strobilurin fungicide. However, little information is so far available regarding the uptake kinetics and developmental toxicity of TFS to fish. The present study was conducted to investigate the uptake kinetics, potential environment risk and toxicity of TFS on Gobiocypris rarus embryos. Results revealed that increased malformation, decreased body length and heart rate, affected spontaneous movement and swimming speed provide a gradual concentration-dependent manner; values of 144 h LC50 (median lethal concentration) and EC50 (median effective concentration) were 1.11 and 0.86 μg/L. Continuous exposure to TFS resulted in a steady accumulation with no evidence of elimination. Enzyme activities were significantly changed; reactive oxygen species and DNA damage were significantly induced after TFS treatment. Certain genes related to cell apoptosis (p53), metabolism (cyp1a), stress response (hsp70) and blood vessels (vezf1) development were all significantly up-regulated. This is the first study to define uptake kinetics and to focus on behavioral consequences, physiological changes and mRNA expression following TFS exposure in the early life stages of fish. Our results suggest that TFS is highly toxic to fish embryos.
    Chemosphere 09/2014; 120:447-455. · 3.14 Impact Factor
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    ABSTRACT: Grass carp reovirus (GCRV), the most pathogenic aquareovirus, can cause fatal hemorrhagic disease in fingerling and yearling grass carp. Vaccination by injection is by far the most effective method of combating disease. However it is labor intensive, costly and not feasible to vaccinate large numbers of the fish. Thus, an efficient and economic strategy for the prevention of GCRV infection becomes urgent. Here, functionalized single-walled carbon nanotubes (SWCNTs) as carrier were used to manufacture SWCNTs-VP7 subunit vaccine with chemical modification. Different developmental stages of grass carps were immunized by VP7/SWCNTs-VP7 subunit vaccine against GCRV by intramuscular injection and bath immunization. The results indicate that better immune responses of grass carp immunized with the SWCNTs-VP7 subunit vaccine were induced in comparison with VP7 subunit vaccine alone. Immunization doses/concentrations are significantly reduced (about 5-8 times) to prevent GCRV infection in different developmental stages of grass carp with injection or bath treatment when SWCNTs carrier was used. A good immune protective effect (relative percentage survival greater than 95%) is observed in smaller size fish (0.2 g) with SWCNTs-VP7 bath immunization. In addition, serum respiratory burst activity, complement activity, lysozyme activity, superoxide dismutase activity, alkaline phosphatase activity, immune-related genes and antibody levels were significantly enhanced in fish immunized with vaccine. This study suggested that functionalized SWCNTs was the promising carrier for recombinant subunit vaccine and might be used to vaccinate fish by bath approach.
    Fish &amp Shellfish Immunology 09/2014; · 2.96 Impact Factor
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    ABSTRACT: Abstract Carbon nanotubes (CNTs) are widely used in industrial and commercial applications, but few studies systematically evaluate their developmental toxicity on aquatic organism. Using rare minnow (Gobiocypris rarus) at early life stages as experimental models, developmental toxicity of functionalized single-walled CNTs (SWCNTs) was investigated following exposure to 0-320 mg/L for 144 h. Results revealed that significantly increased in mortality and malformation was only observed after hatching. Decreased body length, heart rate and swimming speed provide a concentration-dependent manner on larvae; values of 144 h LC50 and EC50 were 140.8 and 109.8 mg/L, respectively. Antioxidant enzyme activities (superoxide dismutase, catalase and glutathione S-transferase) and antioxidant enzyme related mRNA expressions were significant changed; cell apoptosis activities (caspase-3, -8, -9) and cell apoptosis related mRNA expressions were significant up-regulated; reactive oxygen species and DNA damage were significantly induced when the concentration of SWCNTs above 100 mg/L. Fluorescence and electron microscopy sliced observation show that SWCNTs were well dispersed in larvae within 0.5 h, eventually cleared from the larvae at 144 h. This is the first study to define uptake kinetics and to focus on behavioral consequences, physiological changes and mRNA expression following SWCNTs exposure in the early life stages of fish. The results obtained in the present study demonstrated that functionalized SWCNTs have the potential to affect aquatic life when released into the aquatic environment and reached high concentration. In the increasing economical context of SWCNTs, complementary studies must be undertaken, especially including mechanistic and environmental investigations.
    Nanotoxicology 09/2014; · 7.84 Impact Factor
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    Xiao-Bo Yu, Kai Hao, Fei Ling, Gao-Xue Wang
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    ABSTRACT: Cyanobacteria are a diverse group of Gram-negative bacteria that produce an array of secondary compounds with selective bioactivity against vertebrates, invertebrates, fungi, bacteria and cell lines. Recently the main methods of controlling cyanobacteria are using chemicals, medicinal plants and microorganism but fewer involved the safety research in hydrophytic ecosystems. In search of an environmentally safe compound, 53 chemicals were screened against the developed heavy cyanobacteria bloom Microcystis aeruginosa using coexistence culture system assay. The results of the coexistence assay showed that 9 chemicals inhibited M. aeruginosa effectively at 20 mg L(-1) after 7 days of exposure. Among them dimethomorph, propineb, and paraquat were identified that they are safe for Chlorella vulgaris, Scenedesmus obliquus, Carassius auratus (Goldfish) and Bacillus subtilis within half maximal effective concentration (EC50) values 5.2, 4.2 and 0.06 mg L(-1) after 7 days, respectively. Paraquat as the positive control observed to be more efficient than the other compounds with the inhibitory rate (IR) of 92 % at 0.5 mg L(-1). For the potential inhibition mechanism, the chemicals could destroy the cell ultrastructure in different speed. The safety assay proved dimethomorph, propineb and paraquat as harmless formulations or products having potential value in M. aeruginosa controlling, with the advantage of its cell morphology degrading ability.
    Ecotoxicology 08/2014; · 2.77 Impact Factor
  • Bin Zhu, Lei Liu, Yu-Xin Gong, Fei Ling, Gao-Xue Wang
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    ABSTRACT: Using rare minnow (Gobiocypris rarus) at early-life stages as experimental models, the developmental toxicity of five widely used triazole fungicides (myclobutanil, fluconazole, flusilazole, triflumizole, and epoxiconazole) were investigated following exposure to 1-15 mg/L for 72 h. Meanwhile, morphological parameters (body length, body weight, and heart rate), enzyme activities (superoxide dismutase (SOD), glutathione S-transferase (GST), adenosine triphosphatase (ATPase), and acetyl cholinesterase (AChE)), and mRNA levels (hsp70, mstn, mt, apaf1, vezf1, and cyp1a) were also recorded following exposure to 0.2, 1.0, and 5.0 mg/L for 72 h. Results indicated that increased malformation and mortality, decreased body length, body weight, and heart rate provide a concentration-dependent pattern; values of 72 h LC50 (median lethal concentration) and EC50 (median effective concentration) ranged from 3 to 12 mg/L. Most importantly, the results of the present study suggest that even at the lowest concentration, 0.2 mg/L, five triazole fungicides also caused notable changes in enzyme activities and mRNA levels. Overall, the present study points out that those five triazole fungicides are highly toxic to the early development of G. rarus embryos. The information presented in this study will be helpful in better understanding the toxicity induced by triazole fungicides in fish embryos.
    Environmental science and pollution research international. 07/2014;
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    ABSTRACT: The effects of single or combined use of dietary Shewanella haliotis D4, Bacillus cereus D7 and Aeromonas bivalvium D15, isolated from the gut of shrimp, were assessed on the growth, innate immunity and disease resistance of Pacific white shrimp (Litopenaeus vannamei). Shrimp was fed 28 days by five experimental diets — i) control diet with no probiotics, ii) three probiotic diets, i.e. feed supplemented with 107 cell g− 1 of D4 (D4 diet), D7 (D7 diet) and D15 (D15 diet), and iii) mixed diet comprising of strains D4, D7 and D15 at a ratio of 2:1:1, dosed at 107 cell g− 1. Results showed that growth of shrimp, i.e. final weight, weight gain, percent weight gain and specific growth rate, was improved after 28-days of feeding regime with probiotics compared to the control. Respond of several non-specific immune parameters of groups fed probiotics was also enhanced; i.e. diet D4 and D15 led to significant enhancement of both respiratory burst and superoxide dismutase activities than that of control. Moreover, the respiratory burst activity was also enhanced by mixed diet at the end of 28 days. All probiotic groups were able to increase acid phosphatase activity of shrimp between days 7 and 21. Moreover, the expression of prophenoloxidase and < beta>-1, 3-glucan-binding protein was significantly up regulated in shrimp fed D4 and D15 diets compared to control group, but penaeidin 3 expression up regulated significantly only with mixed diet after 28 days. The cumulative mortality of shrimp, experimentally challenged with Vibrio harveyi, was reduced in groups fed probiotics, i.e. D4 = 36.67%, D7 = 41.67%, D15 = 13.33% and mixed = 11.67%, respectively, than that of controls (43.33%) after 10 days of post-infection. Overall, the results of this study indicated the potential of using probiotics to improve growth, immunity and disease resistance of white shrimp.
    Aquaculture 05/2014; s 428–429:141–149. · 2.01 Impact Factor
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    ABSTRACT: This study investigated the effects of trifloxystrobin that one strobilurin used widely in the world as an effective fungicidal agent to control Asian soybean rust on aquatic unicellular algae Chlorella vulgaris. We determined the potential toxic effect of trifloxystrobin on C. vulgaris, and found median inhibition concentration (IC50) value 255.58 (95% confidence interval, 207.81-330.29)μgL(-1). In addition, the algal cells were obviously depressed or shrunk at different concentrations by electron microscopy. In the study, a real-time polymerase chain reaction (PCR) assay showed changes in transcript abundances of three photosynthetic genes, psaB, psbC, and rbcL, and one energy gene, ATPs. The results showed that trifloxystrobin reduced the transcript abundances of the three genes and enhanced expression of ATPs after 48 and 96h. The lowest abundances of psaB, psbC and rbcL transcripts in response to trifloxystrobin exposure were 58%, 79% and 60% of those of the control, respectively. For the potential toxic influences, trifloxystrobin could decrease the soluble protein and total antioxidant contents (T-AOC), and increase superoxide dismutase (SOD) and peroxidase (POD) activity with a gradual concentration-response relationship. Overall, the present study demonstrated that trifloxystrobin could affect the activities of antioxidant enzymes, disrupts photosynthesis in C. vulgaris, and damage cellular structure.
    Environmental toxicology and pharmacology. 04/2014; 37(3):1040-1047.
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    ABSTRACT: Using rare minnow (Gobiocypris rarus) embryos as experimental model, the developmental toxicity of Cu, Zn and Cd were investigated following exposure to 0.001-1.000mg/L for 72h, and the toxicity effects were evaluated by larval malformation rate, heart rate, pericardial area, spontaneous movements, tail length, enzyme activities and biomarker genes. Our results revealed that increased malformation rate provide a gradual dose-response relationship, and the most pronounced morphological alteration was heart and body malformations. Values of 72h EC50 with their 95 percent confidence intervals on G. rarus embryos were 0.103 (0.072-0.149)mg/L for Cu, 0.531 (0.330-1.060)mg/L for Zn, 0.219 (0.147-0.351)mg/L for Cd. Enzyme activities can be regard as a type of low-dose stimulation and high-dose inhibition. Stress and metabolism-related genes (hsp70, cyp1a and mt) were significantly up-regulated, development-related genes (wnt8a, vezf1 and mstn) were significantly down-regulated after the treatment by Cu, Zn and Cd. Overall, the present study points out Cu, Zn and Cd are highly toxic to G. rarus embryos. The information presented in this study will be helpful in fully understanding the toxicity induced by Cu, Zn and Cd in fish embryos.
    Ecotoxicology and Environmental Safety 04/2014; 104C:269-277. · 2.20 Impact Factor
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    ABSTRACT: The study isolated 224 bacteria from the intestine of Apostichopus japonicus, then selected and identified three of the bacteria (HS1, HS7, and HS10) which demonstrated amylase, lipase, and protease production capacity as candidate probiotics for sea cucumbers. The three potential probiotics showed no pathogenicity both in haemolytic assays on sheep blood agar plates and after immersing sea cucumbers in a suspension of the bacteria. To reveal the effects of these three potential probiotics on the innate immunity of sea cucumbers, total coelomocyte counts, respiratory burst activity, superoxide dismutase activity, lysozyme activity, acid phosphatase activity, and phagocytic activity by coelomocytes were examined after feeding with four different diets for up to 28 days. Also the specific growth rate and survival rate were investigated after a 60-day feeding trial. Sea cucumbers were fed with 4 diets: one control, three diets supplemented with 1 × 10(9) cell g(-1) of HS1, HS7, and HS10 for 28-60 days. Results showed that sea cucumbers fed diets containing HS1, HS7, and HS10 had led to an enhanced cellular and humoral immune response, notably higher total coelomocytes counts, respiratory burst activity, lysozyme activity, acid phosphatase activity, and phagocytic activity, as recorded during the four weeks of probiotics administration. On the other hand, the survival rate among dietary treatments ranged from 90.71 to 97.97% with significant improvement (P < 0.05) compared to that of the control; and the growth rate observed in the sea cucumbers fed HS1 and HS7 showed sharp increases after 60 days feeding. The present study confirmed the potential beneficial effects of Pseudoalteromonas elyakovii HS1, Shewanella japonica HS7, and Vibro tasmaniensis HS10 as dietary probiotics in A. japonicus.
    Fish &amp Shellfish Immunology 04/2014; · 2.96 Impact Factor
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    ABSTRACT: Natural plant agents are considered promising sources to control harmful algal blooms. The primary purpose of this study was to isolate the algicidal compounds from Ginkgo biloba exocarp against the toxic cyanobacterium Microcystis aeruginosa and determine the potential inhibition mechanisms. Bioassay-guided fractionation of petroleum ether extract yielded ginkgolic acids, which were identified by spectral analysis (infrared and reversed-phase high-performance liquid chromatography/electrospray mass spectrometry). Bioassays showed ginkgolic acids inhibited M. aeruginosa growth effectively with the 3- and 7-day median effective concentration (EC50,3d, EC50,7d) of 3.26 and 2.03 mg L−1, respectively. For the potential inhibition mechanisms, cellular morphology, physiological changes, and genes expression during 12–96 h of exposure were investigated. Ginkgolic acids caused morphologic damage or lysis of cells. They induced oxidative damage by increasing malondialdehyde content and decreasing total antioxidant and superoxide dismutase activities. Transcription of three photosynthetic genes (psaB, psbD, and rbcL) and two nutrient uptake related genes (ntcA and phoU) were also found to be inhibited. Additionally, ginkgolic acids also affected the microcystin contents and the transcription of microcystin-related genes. The results suggested that ginkgolic acids isolated from G. biloba exocarp have the potential to inhibit M. aeruginosa growth effectively by causing pleiotropic effects on several physiological and biochemical reactions.
    Journal of Applied Phycology 01/2014; · 2.33 Impact Factor
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    ABSTRACT: Triazophos (TAP) has been widely used in agriculture for controlling insect pests and is a known organophosphorus pesticide. Due to TAP characteristics, such as high chemical and photochemical stability, its potential toxicity to aquatic organisms has gained great interest. To explore the potential developmental toxicity of TAP, Gobiocypris rarus embryos and larvae were exposed to various concentrations of TAP (0.1–15 mg L−1) until 72 h. Results showed that values of 72 h LC50 and EC50 were 7.44 and 5.60 mg L−1 for embryos, 2.52 and 1.37 mg L−1 for larvae. Increased malformation, decreased heart rate and body length provide a gradual concentration-dependent pattern. Enzyme activities and mRNA levels were significantly changed even at low concentration (0.05 mg L−1 for embryos and 0.01 mg L−1 for larvae). Overall, the present study points out that TAP is likely a risk to the early development of G. rarus. The information presented in this study will be helpful in better understanding the toxicity induced by TAP in fish embryos and larvae.
    Chemosphere 01/2014; 108:46–54. · 3.14 Impact Factor
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    ABSTRACT: The study isolated 224 bacteria from the intestine of Apostichopus japonicus, then selected and identified three of the bacteria (HS1, HS7, and HS10) which demonstrated amylase, lipase, and protease production capacity as candidate probiotics for sea cucumbers. The three potential probiotics showed no pathogenicity both in haemolytic assays on sheep blood agar plates and after immersing sea cucumbers in a suspension of the bacteria. To reveal the effects of these three potential probiotics on the innate immunity of sea cucumbers, total coelomocyte counts, respiratory burst activity, superoxide dismutase activity, lysozyme activity, acid phosphatase activity, and phagocytic activity by coelomocytes were examined after feeding with four different diets for up to 28 days. Also the specific growth rate and survival rate were investigated after a 60-day feeding trial. Sea cucumbers were fed with 4 diets: one control, three diets supplemented with 1 × 109 cell g-1 of HS1, HS7, and HS10 for 28-60 days. Results showed that sea cucumbers fed diets containing HS1, HS7, and HS10 had led to an enhanced cellular and humoral immune response, notably higher total coelomocytes counts, respiratory burst activity, lysozyme activity, acid phosphatase activity, and phagocytic activity, as recorded during the four weeks of probiotics administration. On the other hand, the survival rate among dietary treatments ranged from 90.71 to 97.97% with significant improvement (P < 0.05) compared to that of the control; and the growth rate observed in the sea cucumbers fed HS1 and HS7 showed sharp increases after 60 days feeding. The present study confirmed the potential beneficial effects of Pseudoalteromonas elyakovii HS1, Shewanella japonica HS7, and Vibro tasmaniensis HS10 as dietary probiotics in A. japonicus.
    Fish &amp Shellfish Immunology 01/2014; · 2.96 Impact Factor
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    ABSTRACT: Using rare minnow (Gobiocypris rarus) embryos as experimental model, the developmental toxicity of Cu, Zn and Cd were investigated following exposure to 0.001–1.000 mg/L for 72 h, and the toxicity effects were evaluated by larval malformation rate, heart rate, pericardial area, spontaneous movements, tail length, enzyme activities and biomarker genes. Our results revealed that increased malformation rate provide a gradual dose–response relationship, and the most pronounced morphological alteration was heart and body malformations. Values of 72 h EC50 with their 95 percent confidence intervals on G. rarus embryos were 0.103 (0.072–0.149) mg/L for Cu, 0.531 (0.330–1.060) mg/L for Zn, 0.219 (0.147–0.351) mg/L for Cd. Enzyme activities can be regard as a type of low-dose stimulation and high-dose inhibition. Stress and metabolism-related genes (hsp70, cyp1a and mt) were significantly up-regulated, development-related genes (wnt8a, vezf1 and mstn) were significantly down-regulated after the treatment by Cu, Zn and Cd. Overall, the present study points out Cu, Zn and Cd are highly toxic to G. rarus embryos. The information presented in this study will be helpful in fully understanding the toxicity induced by Cu, Zn and Cd in fish embryos.
    Ecotoxicology and Environmental Safety 01/2014; 104:269–277. · 2.20 Impact Factor
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    ABSTRACT: Current chemotherapy of mono-component for Dactylogyrus is unsatisfactory due to its limited efficacy with side effects including pesticide resistance and drug residue. The present study is conducted to investigate the effects of herbal ingredients combination against Dactylogyrus on Carassius auratus under in vivo conditions. The ingredients of Dioscorea zingiberensis (D. zingiberensis) and Ginkgo biloba (G. biloba) are administered individually and in combination in an experimental fish infection model in this study. Finally, strong synergy actions are observed in both the methanol extract combinations and the active compound combinations between D. zingiberensis and G. biloba. The anthelmintic effects of the combination are expressed in terms of the values of EC50, EC75, EC90, and EC99 to compare with the phytomedicine alone. The combination index (CI) is also calculated by using the combination index algorithm. Both the values of effective concentration and combination index indicate a highly synergistic anthelmintic effect against Dactylogyrus. Our study provides a powerful means by which anthelmintic combination therapies can be examined and demonstrates that the combinations of herbal ingredients have excellent properties and are predicted to give improved cure rates in monogenean management of aquaculture.
    Aquaculture. 01/2014; 433:115–118.
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    ABSTRACT: The strobilurins are used widely in the world as effective fungicidal agents to control Asian soybean rust. In this study, the early life stage of grass carp (Ctenopharyngodon idella), which is one of the most important aquaculture species in China, was chosen to measure the acute toxicity of three common strobilurin-derived fungicides (trifloxystrobin (TFS), azoxystrobin (AZ) and kresoxim-methyl (KM)). As endpoints, normal developmental parameters (lethal concentration (LC50) and average heart rate), expression of relative genes, and three antioxidant enzyme activities in the developing juveniles were recorded during a 48h exposure. The results revealed that values of LC50 were TFS 0.051 (0.046-0.058) mgL(-1), AZ 0.549 (0.419-0.771) mgL(-1) and KM 0.338 (0.284-0.407) mgL(-1) for juveniles. For the potential toxicity mechanisms, these three fungicides increased catalase (CAT) and peroxidase (POD) activity and decreased superoxide dismutase (SOD) activity, significantly inhibited expressions of three growth-related genes (IGF-1, IGF-2 and GHR) and two energy-related-genes (CCK and PYY), and caused pronounced up-regulation a stress-gene (HSP70). The present study demonstrated potential toxic effects of TFS, AZ and KM on the early development of C. idella. Overall, three strobilurins (TFS, AZ and KM) might cause serious damages to the aquatic species; therefore, their pollution supervision in water ecological environment should be strengthened.
    Ecotoxicology and Environmental Safety 10/2013; · 2.20 Impact Factor
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    ABSTRACT: The study isolated three strains of intestinal autochthonous bacteria Aeromonas veronii BA-1, Vibrio lentus BA-2, and Flavobacterium sasangense BA-3 from the intestinal tract of the common carp (Cyprinus carpio). To reveal the effects of these three strains of bacteria on the innate immunity of carp, the lysozyme, complement C3, total serum protein, albumin and globulin levels, respiratory burst activity, phagocytic activity by blood leucocytes and the expression of IL-1b, lysozyme-C, and TNF-α were exmined after feeding with seven different diets for up to 28 days. Also the survival of carp against Aeromonas hydrophila was challenged for 14 days. The carp were fed seven different diets: one control, three diets supplemented with 1 × 10(8) cell g(-1) of carp intestinal bateria BA-1 (Group D-I), BA-2 (Group D-II) and BA-3 (Group D-III), and three diets supplemented with extracellular products FA-1 (Group E-I), FA-2 (Group E-II) and FA-3 (Group E-III) which were corresponding to the strains BA-1, BA-2, and BA-3, respectively, up to 28 days. For groups D-I, D-III, E-I and E-III, the innate immune parameters of carp were significantly increased, the expression of three immune-related genes in blood was significantly up-regulated examined during 7, 14, and 21 days of feeding, and the survival rate was improved. The study indicates that the two isolated intestinal autochthonous bacteria A. veronii BA-1 and F. sasangense BA-3 could positively influence immune response and enhance disease resistance of carp against A. hydrophila infection.
    Fish &amp Shellfish Immunology 10/2013; · 2.96 Impact Factor
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    ABSTRACT: The purpose of the present study was to assess the anthelmintic property of plant-derived polyphenolic compounds extracted and isolated from Dryopteris crassirhizoma against Dactylogyrus intermedius in goldfish. The active ethyl acetate extract was loaded on an open silica gel column and eluted with chloroform-methanol. According to (1)H-nuclear magnetic resonance (NMR), (13)C-NMR, and mass spectral data, the structures of three purified compounds were identified as protocatechuic acid, sutchuenoside A, and kaempferitrin. Among these compounds, sutchuenoside A and kaempferitrin were observed to be effective with median effective concentration (EC50) of 3.01 and 2.71 mg L(-1), respectively. The alterations in the tegument of the parasites treated with isolated compound were examined using scanning electron microscopes. Ultrastructural micrographs revealed shrinkage of body surface, dense tegumental folds, and disheveled protuberances. The structural deformities in the treated parasites were indicative of an efficient anthelmintic activity of the isolated compound kaempferitrin. In addition, the 48-h median lethal concentration for sutchuenoside A and kaempferitrin against goldfish were 12.03- and 11.98-fold higher than corresponding EC50. The present results showed that ethyl acetate extract of D. crassirhizoma may be considered as a potent source, and sutchuenoside A and kaempferitrin as new natural parasitic agents against D. intermedius.
    Parasitology Research 09/2013; · 2.85 Impact Factor
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    Chao Zhang, Fei Ling, Cheng Chi, Gao-Xue Wang
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    ABSTRACT: Praziquantel and sanguinarine have been demonstrated positive therapeutic effects on monogenean Dactylogyrus intermedius; however, few studies have considered the post impacts of these antiparasitic chemicals on host after repelling the parasites. The changes of expression of selected immune genes (CCL-1, CXCL-8, IL-1β-1, IL-1β-2, TNFα-1, TNFα-2 and TGF-β) in gill, kidney and spleen and bacterial loads of Aeromonas hydrophila in gill, kidney, spleen and liver following bath administration of these antiparasitic chemicals were evaluated. The results showed that praziquantel and sanguinarine up-regulated to varying degrees of CXCL-8, IL-1β-1, IL-1β-2, TNFα-1 and TNFα-2 in gill, kidney and spleen. They both decreased the CCL-1 expression in gill while increased it in kidney and spleen. However, in all the tested tissues, the expression of TGF-β decreased in praziquantel treated goldfish whereas that increased in sanguinarine treated goldfish. The A. hydrophila challenge test showed that the praziquantel treatment enhanced the susceptibility to A. hydrophila while sanguinarine treatment decreased the susceptibility, as compared with the non-treated group. Overall, the results indicate that bath administration of praziquantel and sanguinarine modulates the immune related genes in goldfish and these may, to some extent, affect their ability to resist bacterial pathogens.
    Fish &amp Shellfish Immunology 08/2013; · 2.96 Impact Factor
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    ABSTRACT: The present study was to isolate and identify a potent algicidal compound from extract of Salvia miltiorrhiza and study the potential inhibition mechanism on Microcystis aeruginosa. Column chromatography and bioassay-guided fractionation methods were carried out to yield neo-przewaquinone A, which was identified by spectral analysis. The EC50 of neo-przewaquinone A on M. aeruginosa were 4.68mgL(-1). In addition, neo-przewaquinone A showed relatively higher security on Chlorella pyrenoidosa and Scenedesmus obliquus, with the EC50 values of 14.78 and 10.37mgL(-1), respectively. For the potential inhibition mechanisms, neo-przewaquinone A caused M. aeruginosa cells morphologic damage or lysis, increased malondialdehyde content and decreased the soluble protein content, total antioxidant and superoxide dismutase activity, and significantly inhibited three photosynthesis-related genes (psaB, psbD, and rbcL). The results demonstrated the algicidal effect of neo-przewaquinone A on M. aeruginosa and provided the possible inhibition mechanisms.
    Chemosphere 06/2013; · 3.14 Impact Factor
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    ABSTRACT: The effects of dietary administration of two probiotics, Shewanella colwelliana WA64 and Shewanella olleyana WA65, on the innate immunity of abalone (Haliotis discus hannai Ino), and survival of juvenile abalone challenged with Vibrio harveyi have been studied. Two groups of abalone were fed with three different diets: one control, and two diets supplemented with 10(9) cell g(-1) of probiotic WA64 (WA64 diet) and WA65 (WA65 diet) for up to four weeks. Results showed that abalone fed diets containing S. colwelliana WA64 and S. olleyana WA65 had led to an enhanced cellular and humoral immune response, notably higher haemocytes, respiratory burst activity, serum lysozyme activity and total protein levels were recorded after one week of probiotic administration. On the other hand, mortality after the challenges with V. harveyi in the group fed with control diet ranged from 77-80%, while mortality rates observed in the groups fed with diets supplemented with WA64 and WA65 ranged from 27-50% and 30-43%, respectively. The results demonstrated potential for Shewanella colwelliana WA64 and Shewanella olleyana WA65 to improve innate immunity and disease resistance in H. discus hannai.
    Fish &amp Shellfish Immunology 04/2013; · 2.96 Impact Factor

Publication Stats

145 Citations
103.96 Total Impact Points

Institutions

  • 2014
    • Northwest University
      Northwest Harborcreek, Pennsylvania, United States
  • 2013
    • Chinese Academy of Fishery Sciences
      北江, Zhejiang Sheng, China
  • 2009
    • Northwest A & F University
      • College of Animal Science and Technology
      Yangling, Shaanxi Sheng, China