Reiner M Kroppenstedt

Publications of Reiner M Kroppenstedt

• Micromonospora cremea sp. nov. and Micromonospora zamorensis sp. nov., isolated from the rhizosphere of Pisum sativum.

  Authors: Lorena Carro, Rüdinger Pukall, Cathrin Spröer, Reiner M Kroppenstedt, Martha E Trujillo

  International journal of systematic and evolutionary microbiology. 01/2012;

  Three actinobacterial strains, CR30T, CR36 and CR38T were isolated from the rhizosphere soil of Pisum sativum plants collected in Spain. The strains were filamentous, Gram-positive and producedThree actinobacterial strains, CR30T, CR36 and CR38T were isolated from the rhizosphere soil of Pisum sativum plants collected in Spain. The strains were filamentous, Gram-positive and produced single spores. Phylogenetic, chemotaxonomic and morphological analyses confirmed that the three strains belonged to the genus Micromonospora. 16S rRNA gene sequences of strains CR30T and CR36 showed closest relationship with Micromonospora coriariae NAR01T (99.3% similarity) while strain CR38T had a similarity of 99.0% with Micromonospora saelicesensis Lupac 09T. In addition, gyrB gene phylogeny clearly differentiated the new isolates from established Micromonospora species. DNA-DNA hybridization, BOX PCR and ARDRA profiles confirmed that these strains represent new genomic species. The cell-wall peptidoglycan of strains CR30T and CR38T contained meso-DAP. Both strains had MK-10(H4) as the main menaquinone and a phospholipid type II pattern. An array of physiological tests also differentiated the new isolates from their closest neighbours. Considering all the data obtained, it is proposed that strains CR30T and CR36 represent a new species under the name Micromonospora cremea sp. nov. (type strain CR30T = CECT 7891T = DSM 45599T), while the name Micromonospora zamorensis sp. nov. is proposed with isolate CR38T (= CECT 7892T = DSM 45600T) as the type strain.

• Nocardia mikamii sp. nov., isolated from human pulmonary infections in the United States.

  Authors: Deanna P Jannat-Khah, Reiner M Kroppenstedt, Hans-Peter Klenk, Cathrin Spröer, Peter Schumann, Brent A Lasker, Arnold G Steigerwalt, Hans P Hinrikson, June M Brown

  International journal of systematic and evolutionary microbiology. 11/2009;

  Four nocardioform bacteria were isolated from clinical respiratory sources (W7467, W7811, W8061T and W9013) in the United States. Macroscopic examination showed scant aerial hyphae and beige-redFour nocardioform bacteria were isolated from clinical respiratory sources (W7467, W7811, W8061T and W9013) in the United States. Macroscopic examination showed scant aerial hyphae and beige-red substrate hyphae. They showed chemotaxonomic markers that were consistent with the classification of Nocardia: i.e., meso-diaminopimelic acid; arabinose and galactose as diagnostic sugars; the phospholipids diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides; a menaquinone with a omega-cyclic isoprene side chain MK-8(H4cycl.); a fatty acid pattern composed of unbranched saturated and monounsaturated fatty acids with considerable amount of tuberculostearic acid; and mycolic acids comprising 52 to 62 carbon atoms with three principal mycolic acids which were mono- and polyunsaturated showing a chain length of C54, C56 and C58. 16S rRNA gene sequence data and phenotypic characters showed they were most closely related to Nocardia aobensis (DSM 44805T ). The G+C content was 68.3 mol %. Analysis of a 1,245-bp fragment of gyrB gene showed a clade separate from N. aobensis and was supported by the DNA relatedness of 67 % of W8061T to N. aobensis. These data indicated that the novel isolates represent a new species within the genus Nocardia, for which the name Nocardia mikamii sp. nov. is proposed, with W8061T (DSM 45174T = JCM 15508T) designated as the type strain.

• Nocardiopsis sinuspersici sp. nov., isolated from the sandy soil of Iran.

  Authors: Javad Hamedi, Fatemeh Mohammadipanah, Mathias von Jan, Gabriele Pötter, Peter Schumann, Cathrin Spröer, Hans-Peter Klenk, Reiner M Kroppenstedt

  International journal of systematic and evolutionary microbiology. 11/2009;

  A polyphasic taxonomic study of a halotolerant bacterium, isolated from sandyrhizosphaeral soil in Sarbandar, Persian Gulf, Iran revealed that strain HM6T represents a novel species within the genusA polyphasic taxonomic study of a halotolerant bacterium, isolated from sandyrhizosphaeral soil in Sarbandar, Persian Gulf, Iran revealed that strain HM6T represents a novel species within the genus Nocardiopsis. Results of the 16S rRNA gene sequence comparison revealed that strain HM6T clustered with strains of the genus Nocardiopsis showing the highest degree of 16S rRNA gene sequence similarity to N. quinghaiensis (99.2 %), N. aegyptia (98.5 %) and N. halotolerans (98.3 %). However, DNA-DNA hybridization studies with these type strains revealed less than 39.6% similarity. Rather than genotypic difference there are some phenotypic discrepancies between strain HM6T and closely related Nocardiopsis species. Main morphological and chemotaxonomical features of strain HM6T including: (i) growth characteristics, i.e. the formation of a scant light-yellow to white aerial mycelium and the typical zig-zag form of the hyphae, which fragment during ageing into smooth rod shaped spores; (ii) the presence of meso-diaminopimelic acid and glucose plus ribose in whole-cell hydrolyzates; (iii) the presence of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol together with three unknown Nocardiopsis specific phospholipids (close to diphosphatidylglycerol position) in polar lipid extracts; (iv) the presence of two major menaquinones MK-10(H2), MK-10(H2) and MK-9(H0) in the non-polar fraction; (v) the presence of iso/anteiso-branched plus 10-methyl-branched fatty acids, showing the diagnostic combination for Nocardiopsis spp. of iso-16:0 (31.1 %), anteiso-17:0 (19.2 %), 10-methyl-17:0 (5.8 %) and tuberculostearic acid (8.8%); and (vi) the absence of mycolic acids. Analysis of 16S rRNA gene revealed that strain HM6T represents a distinct taxon within Nocardiopsis. Based upon genotypic and phenotypic differences to other members of the genus, a novel species, Nocardiopsis sinuspersici sp. nov., is proposed. The type strain is HM6T (= UTMC 00102T = DSM 45277T = CCUG 57624T).

• Tsukamurella soli sp. nov., isolated from soil.

  Authors: Hang-Yeon Weon, Seung-Hee Yoo, Rangasamy Anandham, Peter Schumann, Reiner M Kroppenstedt, Soon-Wo Kwon, Erko Stackebrandt

  International journal of systematic and evolutionary microbiology. 10/2009;

  One white-colored bacterium designated as strain JS18-1T was isolated from a soil sample collected from Halla mountain in Jeju island, Republic of Korea. Cells of the strain were Gram-positive,One white-colored bacterium designated as strain JS18-1T was isolated from a soil sample collected from Halla mountain in Jeju island, Republic of Korea. Cells of the strain were Gram-positive, rod-shaped, catalase-positive and oxidase-negative. 16S rRNA gene sequence analysis revealed that the strain was most closely related to members of the genus Tsukamurella with the sequence similarities of 96.4-95.2%. Strain JS18-1T shared the highest sequence similarities with Tsukamurella strandjordii DSM 44573T (96.4%), Tsukamurella carboxydivorans Y2T (96.3%) and Tsukamurella tyrosinosolvens DSM 44234T (96.3%). The G+C content of the total DNA was 70 mol%. Cell-wall peptidoglycan type was A1gamma and mycolic acids were also detected. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major quinone and major cell-wall sugars were MK-9, and arabinose, ribose and glucose, respectively. The major fatty acids (>10 % of the total fatty acids) were C16:0, C18:1 omega9c, C18:0 10-methyl and summed feature 3 (C16:1 omega7c and/or iso-C 15:0 2-OH). It is evident from the phylogenetic analysis based on the 16S rRNA gene sequences, and chemotaxonomic, biochemical and physiological characteristics that strain JS18-1T represents a novel species of the genus Tsukamurella, for which the name Tsukamurella soli sp. nov. is proposed. The type strain is JS18-1T (=KACC 20764T=DSM 45046T).

• Streptomyces iranensis sp. nov., a novel Streptomycete isolated from soil in Iran.

  Authors: Javad Hamedi, Fatemeh Mohammadipanah, Hans-Peter Klenk, Gabriele Pötter, Peter Schumann, Cathrin Spröer, Mathias von Jan, Reiner M Kroppenstedt

  International journal of systematic and evolutionary microbiology. 09/2009;

  The taxonomic position of a Streptomycete was determined. The strain HM 35T was isolated from soil in Isfahan an Iranian city. Strain HM 35T produced a branched substrate and aerial mycelium thatThe taxonomic position of a Streptomycete was determined. The strain HM 35T was isolated from soil in Isfahan an Iranian city. Strain HM 35T produced a branched substrate and aerial mycelium that developed into short compact spiral spore chains with gray smooth spores at the tips of the aerial hyphae. On some media these spirals may coalesce as dark masses of spores during ageing. Whole cell hydrolyzates of strain HM 35T contained LL-diaminopimelic acid, glucose and ribose. The phospholipids found were phosphatidylglycerol, diphosphatidylglycerol, phosphatidyinositol phosphatidylethanolamine and hydroxy-phosphatidylethanolamine; MK-9(H4), MK-9(H6) and MK-9 (H8) were the predominant menaquinones. The fatty acid pattern was composed of iso- and anteiso-branched fatty acids. The chemotaxonomic characteristics of the isolate matched those described for members of the genus Streptomyces. Based on its 16S rRNA analysis strain HM 35T showed the most relationship with Streptomyces rapamycinicus NRRLB-5491T (99.2 %), Streptomyces violaceusniger DSM 40563 (99.1%), Streptomyces javensis DSM41764 (99.1 %) and Streptomyces yogyakartensis DSM 41766 (99.1 %). The strain formed a distinct monophyletic line within the 16S rRNA gene sequence phylogenetic tree. The DNA-DNA hybridization studies showed 72.7 % homology to Streptomyces rapamycinicus. HM 35T shows the typical morphology found among members of the Streptomyces violaceusniger/S. hygroscopicus group but can be well differentiated from closely related species by other phenotypic markers. On the results of phenotypic and genotypic studies, strain HM 35T (Type strain HM 35T = DSM 41954 T = CCUG 57623T) is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces iranensis is proposed.

• Streptomyces atriruber sp. nov. and Streptomyces silaceus sp. nov., of equine origin.

  Authors: David P Labeda, Neil P Price, Reiner M Kroppenstedt, James Michael Donahue, Neil M Williams, Stephen F Sells

  International journal of systematic and evolutionary microbiology. 08/2009;

  Actinomycete strains isolated from lesions on equine placentas in Kentucky were subjected to polyphasic taxonomic identification. On the basis of phylogenetic analysis of 16S rRNA gene sequences,Actinomycete strains isolated from lesions on equine placentas in Kentucky were subjected to polyphasic taxonomic identification. On the basis of phylogenetic analysis of 16S rRNA gene sequences, morphological observations, and the presence of the LL-diaminopimelic acid as the diamino-acid in whole-cell hydrolysates, the isolates clearly belong to the genus Streptomyces. Analyses of the phylogenetic position of strains NRRL B-24165 and NRRL B-24166 based on 16S rRNA gene sequences of all Streptomyces species as well as evaluation of morphological and physiological characteristics demonstrated that they could be differentiated from all known species and therefore represent novel species. It is proposed that new species be described for these strains, Streptomyces atriruber sp. nov. (type strain NRRL B-24165T =DSM 41860T) and Streptomyces silaceus sp. nov. (type strain NRRL B-24166T =DSM 41861T) These species names are based on the distinctive color of the substrate mycelium of these strains, dark red and deep orange-yellow, respectively.

• Mycetocola reblochoni sp. nov., isolated from the surface microbial flora of Reblochon cheese.

  Authors: Nagamani Bora, Marc Vancanneyt, Roberto Gelsomino, Cindy Snauwaert, Jean Swings, Amanda L Jones, Alan C Ward, Jean-Francois Chamba, Reiner M Kroppenstedt, Peter Schumann, Michael Goodfellow

  International journal of systematic and evolutionary microbiology. 01/2009; 58(Pt 12):2687-93.

  Four Gram-positive, aerobic, non-sporulating, rod-shaped bacteria isolated from the surface microflora of Reblochon cheese at the late stage of ripening had chemotaxonomic properties characteristicFour Gram-positive, aerobic, non-sporulating, rod-shaped bacteria isolated from the surface microflora of Reblochon cheese at the late stage of ripening had chemotaxonomic properties characteristic of members of the family Microbacteriaceae. The isolates had virtually identical SDS-PAGE whole-organism protein patterns, shared many chemical and phenotypic characteristics and formed an independent branch in the Microbacteriaceae 16S rRNA gene tree that was most closely related to the type strains of Mycetocola species. The new isolates had chemotaxonomic properties consistent with their classification in the genus Mycetocola but were readily distinguished from recognized members of this taxon based on DNA-DNA relatedness, whole-organism protein and phenotypic data. The combined genotypic and phenotypic data indicate that the isolates should be classified in the genus Mycetocola as members of a novel species, for which the name Mycetocola reblochoni sp. nov. is proposed. The type strain is LMG 22367(T) (=R-20377(T) =BRB-1L41(T) =DSM 18580(T)).

• Cellulomonas aerilata sp. nov., isolated from an air sample.

  Authors: Chang-Muk Lee, Hang-Yeon Weon, Seung-Beom Hong, Young-Ah Jeon, Peter Schumann, Reiner M Kroppenstedt, Soon-Wo Kwon, Erko Stackebrandt

  International journal of systematic and evolutionary microbiology. 01/2009; 58(Pt 12):2925-9.

  A Gram-positive, aerobic, motile, coccoid or short rod-shaped bacterium, 5420S-23(T), was isolated from an air sample collected in the Republic of Korea. According to phylogenetic analysis based onA Gram-positive, aerobic, motile, coccoid or short rod-shaped bacterium, 5420S-23(T), was isolated from an air sample collected in the Republic of Korea. According to phylogenetic analysis based on 16S rRNA gene sequences, strain 5420S-23(T) revealed 97.5, 97.3, 97.3 and 97.2 % similarity, respectively, to Cellulomonas biazotea DSM 20112(T), Cellulomonas cellasea DSM 20118(T), Cellulomonas fimi DSM 20113(T) and Cellulomonas chitinilytica X.bu-b(T). The peptidoglycan type of strain 5420S-23(T) was A4beta, containing l-ornithine-d-glutamic acid. The cell-wall sugars were galactose, glucose and xylose. The major fatty acids were anteiso-C(15 : 0) (49.7 %) and C(16 : 0) (20.0 %). The major menaquinone was MK-9(H(4)) and major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 74 mol%. The results of DNA-DNA hybridization with strains of closely related Cellulomonas species, in combination with chemotaxonomic and physiological data, demonstrated that isolate 5420S-23(T) represents a novel Cellulomonas species, for which the name Cellulomonas aerilata sp. nov. is proposed, with strain 5420S-23(T) (=KACC 20692(T) =DSM 18649(T)) as the type strain.

• Reclassification of Myxococcus flavescens Yamanaka et al. 1990VP as a later synonym of Myxococcus virescens Thaxter 1892AL.

  Authors: Elke Lang, Reiner M Kroppenstedt, Bettina Sträubler, Erko Stackebrandt

  International journal of systematic and evolutionary microbiology. 12/2008; 58(Pt 11):2607-9.

  The taxonomic relatedness between the species Myxococcus flavescens and Myxococcus virescens was investigated. Literature data had already indicated the synonymy between the two species but thisThe taxonomic relatedness between the species Myxococcus flavescens and Myxococcus virescens was investigated. Literature data had already indicated the synonymy between the two species but this observation had not been formalized. Additional evidence that the two taxa represent a single species was provided by comparison of metabolic properties, cellular fatty acid profiles and from a DNA-DNA reassociation value of >80 %. Data from this study led to the proposal that M. flavescens should be reclassified as a later synonym of M. virescens.

• Phycicoccus aerophilus sp. nov., isolated from air.

  Authors: Hang-Yeon Weon, Seung-Hee Yoo, Byung-Yong Kim, Peter Schumann, Reiner M Kroppenstedt, Sung Kee Hong, Soon-Wo Kwon

  International journal of systematic and evolutionary microbiology. 11/2008; 58(Pt 10):2389-92.

  A Gram-positive, short rod-shaped, non-spore-forming bacterium, designated 5516T-20(T), was isolated from an air sample. The results of 16S rRNA gene sequence analyses showed that strain 5516T-20(T)A Gram-positive, short rod-shaped, non-spore-forming bacterium, designated 5516T-20(T), was isolated from an air sample. The results of 16S rRNA gene sequence analyses showed that strain 5516T-20(T) belonged to the family Intrasporangiaceae, having the highest sequence similarities (97.5 and 96.1 %, respectively) with respect to the type strains of Phycicoccus dokdonensis and Phycicoccus jejuensis. The value for DNA-DNA hybridization between 5516T-20(T) and P. dokdonensis DS-8(T) was 41 %. Strain 5516T-20(T) contained menaquinone MK-8(H(4)) as the major isoprenoid quinone, possessed phosphatidylethanolamine, phosphatidylinositol and diphosphatidylglycerol as the polar lipids and contained glucose and ribose as whole-cell sugars. The major fatty acids were C(17 : 1)omega8c, iso-C(16 : 0), iso-C(15 : 0), C(17 : 0) and iso-C(14 : 0). The G+C content of the DNA from strain 5516T-20(T) was 70.5 mol%. On the basis of the data from the polyphasic taxonomic study, strain 5516T-20(T) represents a novel species within the genus Phycicoccus, for which the name Phycicoccus aerophilus sp. nov. is proposed. The type strain is 5516T-20(T) (=KACC 20658(T) =DSM 18548(T)).

• Nocardia altamirensis sp. nov., isolated from Altamira cave, Cantabria, Spain.

  Authors: Valme Jurado, Patrick Boiron, Reiner M Kroppenstedt, Frédéric Laurent, Andrée Couble, Leonila Laiz, Hans-Peter Klenk, Juan M González, Cesareo Saiz-Jimenez, Delphine Mouniée, Emanuelle Bergeron, Verónica Rodríguez-Nava

  International journal of systematic and evolutionary microbiology. 10/2008; 58(Pt 9):2210-4.

  A novel actinomycete strain, OFN S17(T), was isolated from a sample collected from Altamira Cave, Cantabria, Spain. This strain was identified by using a polyphasic taxonomic approach. The 16S rRNA,A novel actinomycete strain, OFN S17(T), was isolated from a sample collected from Altamira Cave, Cantabria, Spain. This strain was identified by using a polyphasic taxonomic approach. The 16S rRNA, hsp65 and sod gene sequences of the strain were determined and compared with those of representative Nocardia species. The results showed that strain OFN S17(T) should be assigned to the genus Nocardia. Phylogenetic analysis indicated that strain OFN S17(T) was most closely related to the type strain of Nocardia tenerifensis (98.6, 96.2 and 96 % similarity, respectively, for the 16S rRNA, hsp65 and sod gene sequences). The DNA G+C content was 64.4 mol%. DNA-DNA hybridization analyses revealed 29 % relative reassociation between the DNA of strain OFN S17(T) and N. tenerifensis DSM 44704(T). The phenotypic and genotypic data show that strain OFN S17(T) merits recognition as a representative of a novel species of the genus Nocardia, for which the name Nocardia altamirensis sp. nov. is proposed. The type strain is OFN S17(T) (=CIP 109606(T) =DSM 44997(T)).

• The temperature-adaptive fatty acid content in Bacillus simplex strains from 'Evolution Canyon', Israel.

  Authors: Johannes Sikorski, Evelyne Brambilla, Reiner M Kroppenstedt, Brian J Tindall

  Microbiology (Reading, England). 09/2008; 154(Pt 8):2416-26.

  Exploring the evolutionary response of Bacillus simplex strains to the slope-specific habitats of 'Evolution Canyon' I and II, Israel, we report here on presumably adaptive differences in fatty acidExploring the evolutionary response of Bacillus simplex strains to the slope-specific habitats of 'Evolution Canyon' I and II, Israel, we report here on presumably adaptive differences in fatty acid (FA) content that correlate with one particular feature of the habitats, temperature difference. These two canyons represent similar ecological sites, separated by 40 km, in which the orientation of the sun yields a strong sun-exposed and hot 'African' south-facing slope versus a rather cooler and mesic-lush 'European' north-facing slope within a distance of only 50-400 m. Among 131 strains, which are identical in their 16S sequences, those assigned genetically to the 'African' ecotypes express phenotypically generally more high-temperature-tolerance-providing iso-branched FAs than strains assigned to the 'European' ecotypes when grown at 20 degrees C, 28 degrees C and 40 degrees C. Conversely, 'European' lineages express larger amounts of low-temperature-tolerance-providing anteiso-branched and non-saturated FAs when grown at the same temperatures. Moreover, 'African' ecotypes show a stronger adjustment of their high- and low-temperature-tolerance-providing FAs in response to low temperatures, which suggests that, as a result of temperature adaptation, 'African' and 'European' ecotypes have evolved different reaction norms within their phenotypic plasticity response. Thus, bacterial adaptive microevolution may include such multigenic and highly complex organs as the bacterial cell membrane. The results contribute to our understanding of the speciation process among the 'Evolution Canyon' B. simplex ecotypes.

• Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal- and sulfate-containing wastewater.

  Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M Kroppenstedt, Jaakko A Puhakka

  International journal of systematic and evolutionary microbiology. 05/2008; 58(Pt 4):833-8.

  A moderately thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and sulfate. The strain,A moderately thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and sulfate. The strain, designated RE35E1T, was rod-shaped and motile. The temperature range for growth was 33-51 degrees C (optimum 44-46 degrees C) and the pH range was 6.0-7.5 (optimum pH 6.4-7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM sulfate, thiosulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained meso-diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 1 omega 10c and iso-C17 : 0. Strain RE35E1T was related to representatives of the genera Desulfotomaculum and Sporotomaculum, the closest relatives being Desulfotomaculum arcticum DSM 17038T (96.3 % 16S rRNA gene sequence similarity) and Sporotomaculum hydroxybenzoicum DSM 5475T (92.0 % similarity). Strain RE35E1T represents a novel species, for which the name Desulfotomaculum alcoholivorax sp. nov. is proposed. The type strain is RE35E1T (=DSM 16058T=JCM 14019T).

• Nocardiopsis quinghaiensis sp. nov., isolated from saline soil in China.

  Authors: Yi-Guang Chen, Xiao-Long Cui, Reiner M Kroppenstedt, Erko Stackebrandt, Meng-Liang Wen, Li-Hua Xu, Cheng-Lin Jiang

  International journal of systematic and evolutionary microbiology. 04/2008; 58(Pt 3):699-705.

  A previously unknown Gram-positive, obligately aerobic actinomycete, YIM 28A4(T), was isolated from a sample of saline soil collected from the Qaidam Basin in Qinghai Province, north-west China, andA previously unknown Gram-positive, obligately aerobic actinomycete, YIM 28A4(T), was isolated from a sample of saline soil collected from the Qaidam Basin in Qinghai Province, north-west China, and was investigated using a polyphasic taxonomic approach. The strain grew well on most of the media tested, producing white to pale-yellow substrate mycelium, white aerial mycelium and straight to flexuous hyphae. The substrate mycelium was well developed and fragmented with age; the aerial mycelium produced long, straight spore chains. The spore chains were composed of non-motile, smooth-surfaced, rod-shaped spores. No diffusible pigments were produced on any of the media tested. The strain grew in the presence of 0-10 % (w/v) NaCl and at pH 6.0-8.0, with optimum growth occurring at 3 % (w/v) NaCl and pH 7.0. It grew at 10-37 degrees C, the optimum growth temperature being 28 degrees C. Whole-cell hydrolysates of strain YIM 28A4(T) contained meso-diaminopimelic acid and no diagnostic sugars. The predominant phospholipids were phosphatidylcholine, phosphatidylglycerol and diphosphatidylglycerol. The predominant menaquinones were MK-10, MK-10(H(2)), MK-11 and MK-11(H(2)). The major cellular fatty acids were iso-C(16 : 0), anteiso-C(15 : 0) and anteiso-C(17 : 0). The DNA G+C content was 67.1 mol%. The morphological and chemotaxonomic characteristics of the isolate matched those described for Nocardiopsis species. A phylogenetic analysis based on 16S rRNA gene sequence comparisons confirmed that strain YIM 28A4(T) was a member of the genus Nocardiopsis and most closely related to the type strains Nocardiopsis aegyptia DSM 44442(T) and Nocardiopsis halotolerans DSM 44410(T), showing 98.1 and 97.8 % 16S rRNA gene sequence similarity, respectively. Strain YIM 28A4(T) can be differentiated from these type strains by using phenotypic, phylogenetic and DNA-DNA hybridization data. On the basis of the polyphasic evidence, strain YIM 28A4(T) represents a novel species of the genus Nocardiopsis, for which the name Nocardiopsis quinghaiensis sp. nov. is proposed. The type strain is YIM 28A4(T) (=DSM 44739(T) =CGMCC 4.3494(T)).

• Bacillus butanolivorans sp. nov., a species with industrial application for the remediation of n-butanol.

  Authors: Nomeda Kuisiene, Juozas Raugalas, Cathrin Spröer, Reiner M Kroppenstedt, Donaldas Chitavichius

  International journal of systematic and evolutionary microbiology. 03/2008; 58(Pt 2):505-9.

  Four bacterial strains, designated K9(T), K105, K1012A and K101, were isolated from soil in Lithuania. All these strains could use n-butanol as a sole carbon source. The strains grew in a mediumFour bacterial strains, designated K9(T), K105, K1012A and K101, were isolated from soil in Lithuania. All these strains could use n-butanol as a sole carbon source. The strains grew in a medium containing 12-120 mM n-butanol. The strains were strictly aerobic, Gram-positive endospore-formers. The best growth was achieved at 25 degrees C and pH 7.0 in medium containing 1 % (w/v) NaCl. The strains showed identical profiles of 16S-23S rRNA internal transcribed spacer PCR and nearly identical 16S rRNA gene PCR-RFLP electrophoretic patterns and physiological characteristics, demonstrating their relationship at the species level. The cellular fatty acid profile of K9(T) consisted of significant amounts of the C(15) branched-chain fatty acids iso-C(15 : 0) (16.78 %) and anteiso-C(15 : 0) (45.80 %). The diagnostic cell-wall diamino acid was meso-diaminopimelic acid. The 16S rRNA gene sequence of K9(T) showed the highest similarity to the sequences of Bacillus simplex DSM 1321(T) and Bacillus muralis LMG 20238(T) (98.3 and 97.7 %, respectively). The DNA G+C content was 37.4 mol%. Studies of DNA-DNA relatedness, morphological, physiological and chemotaxonomic analyses and phylogenetic data based on 16S rRNA gene sequencing allowed strains K9(T), K105, K1012A and K101 to be described as members of a novel species of the genus Bacillus, for which the name Bacillus butanolivorans sp. nov. is proposed. The type strain is K9(T) (=DSM 18926(T) =LMG 23974(T)).

• Knoellia aerolata sp. nov., isolated from an air sample in Korea.

  Authors: Hang-Yeon Weon, Byung-Yong Kim, Peter Schumann, Reiner M Kroppenstedt, Hyung-Jun Noh, Chan-Won Park, Soon-Wo Kwon

  International journal of systematic and evolutionary microbiology. 01/2008; 57(Pt 12):2861-4.

  An aerobic, Gram-positive, non-motile, non-spore-forming, rod-coccus-shaped bacterium, strain 5317S-21(T), was isolated from an air sample from Suwon city, Republic of Korea. The isolate was able toAn aerobic, Gram-positive, non-motile, non-spore-forming, rod-coccus-shaped bacterium, strain 5317S-21(T), was isolated from an air sample from Suwon city, Republic of Korea. The isolate was able to grow within a pH range of 5.0-9.0 and a temperature range of 5-35 degrees C and it tolerated up to 2 % (w/v) NaCl. The cell-wall peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. The predominant isoprenoid quinone was MK-8(H(4)). The major polar lipids were phosphatidylinositol, phosphatidylethanolamine and diphosphatidylglycerol; phosphatidylglycerol and several unknown phospholipids were also detected. Mycolic acids were absent. The only whole-cell sugar was glucose. The major cellular fatty acids were iso-C(16 : 0), C(17 : 1)omega8c and iso-C(15 : 0). 16S rRNA gene sequence analysis indicated that strain 5317S-21(T) was related phylogenetically to members of the genus Knoellia, with 97.4 % sequence similarity to the type strains of Knoellia sinensis and Knoellia subterranea. The G+C content of the genomic DNA of strain 5317S-21(T) was 73 mol%. Levels of DNA-DNA relatedness between strain 5317S-21(T) and the type strains of Knoellia sinensis and Knoellia subterranea were 37 and 41 %, respectively. It was concluded that strain 5317S-21(T) represents a novel species of the genus Knoellia, for which the name Knoellia aerolata sp. nov. is proposed. The type strain is 5317S-21(T) (=KACC 20583(T) =DSM 18566(T)).

• Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius.

  Authors: Martha E Trujillo, Reiner M Kroppenstedt, Carmen Fernández-Molinero, Peter Schumann, Eustoquio Martínez-Molina

  International journal of systematic and evolutionary microbiology. 01/2008; 57(Pt 12):2799-804.

  A study was conducted to determine the taxonomic status of six actinomycete strains isolated from root nodules of Lupinus angustifolius. The strains were filamentous, Gram-positive and producedA study was conducted to determine the taxonomic status of six actinomycete strains isolated from root nodules of Lupinus angustifolius. The strains were filamentous, Gram-positive and produced single spores at the tip of the hyphae. Phylogenetic, chemotaxonomic and morphological analyses demonstrated that all six strains belonged to the genus Micromonospora. According to the 16S rRNA gene sequence data, the strains were divided into two clusters that are moderately related to Micromonospora mirobrigensis, Micromonospora matsumotoense and Micromonospora purpureochromogenes. Fatty acid patterns also supported the division of the strains, and significant differences between the two groups were found in the amounts of iso-15 : 0, iso-16 : 0, iso-16 : 1 and iso-17 : 0. Furthermore, the two groups showed physiological differences which included utilization of arabinose, trehalose, alanine and sucrose and xylan hydrolysis. Finally, DNA-DNA hybridization and ribotyping studies confirmed that each group represents a novel species. Based on the genotypic and phenotypic data, the novel species Micromonospora lupini sp. nov. (type strain Lupac 14N(T) =DSM 44874(T) =LMG 24055(T)) and Micromonospora saelicesensis sp. nov. (type strain Lupac 09(T) =DSM 44871(T) =LMG 24056(T)) are proposed.

• Terrabacter aerolatus sp. nov., isolated from an air sample.

  Authors: Hang-Yeon Weon, Peter Schumann, Reiner M Kroppenstedt, Byung-Yong Kim, Jaekyeong Song, Soon-Wo Kwon, Seung-Joo Go, Erko Stackebrandt

  International journal of systematic and evolutionary microbiology. 10/2007; 57(Pt 9):2106-9.

  A Gram-positive, strictly aerobic, motile, rod- or coccoid-shaped bacterium, strain 5516J-36(T), was isolated from an air sample from Jeju region, Korea, and its taxonomic position was investigatedA Gram-positive, strictly aerobic, motile, rod- or coccoid-shaped bacterium, strain 5516J-36(T), was isolated from an air sample from Jeju region, Korea, and its taxonomic position was investigated by a polyphasic approach. The organism grew optimally at 30 degrees C and pH 7.0-8.0. Comparative 16S rRNA gene sequencing studies demonstrated that this strain was highly related phylogenetically to Terrabacter terrae PPLB(T) and Terrabacter tumescens DSM 20308(T), showing 98.9 % sequence similarity to both strains. However, the DNA-DNA reassociation values between 5516J-36(T) and the type strains of Terrabacter terrae and Terrabacter tumescens were low (51 and 48 %, respectively). The peptidoglycan type was A3gamma, the predominant menaquinone was MK-8(H(4)), the polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and an unidentified phosphoglycolipid and the whole-cell sugars were glucose, ribose, rhamnose, xylose and galactose. Mycolic acids were absent. The major fatty acids (>5 % of total fatty acids) were iso-C(15 : 0), iso-C(16 : 0), iso-C(14 : 0), iso-C(17 : 0) and anteiso-C(15 : 0). The DNA G+C content was 71.7 mol%. On the basis of the above data, it is proposed that strain 5516J-36(T) represents a novel species, Terrabacter aerolatus sp. nov. The type strain of Terrabacter aerolatus is 5516J-36(T) (=KACC 20556(T) =DSM 18562(T)).

• Nocardia terpenica sp. nov., isolated from Japanese patients with nocardiosis.

  Authors: Yasutaka Hoshino, Kayo Watanabe, Soji Iida, Shin-ichi Suzuki, Takuji Kudo, Takahisa Kogure, Katsukiyo Yazawa, Jun Ishikawa, Reiner M Kroppenstedt, Yuzuru Mikami

  International journal of systematic and evolutionary microbiology. 08/2007; 57(Pt 7):1456-60.

  Two bacterial strains isolated from different hospitals in Japan were subjected to a polyphasic analysis. Strains IFM 0406 and IFM 0706(T), producers of novel terpenoid antibiotics, were found toTwo bacterial strains isolated from different hospitals in Japan were subjected to a polyphasic analysis. Strains IFM 0406 and IFM 0706(T), producers of novel terpenoid antibiotics, were found to have morphological, biochemical, physiological and chemotaxonomic properties consistent with their classification in the genus Nocardia, except for the presence of MK-8(H(4)) as one of the predominant menaquinones in addition to the major menaquinone MK-8(H(4 omega-cyc)). The 16S rRNA gene sequence similarity of strains IFM 0406 and IFM 0706(T) was 99.9 %, and the closest members of Nocardia to these strains were the type strains of Nocardia nova and Nocardia mexicana, showing similarity of 97.5 and 97.1 %, respectively. Based on their characteristic phenotypic and phylogenetic properties, a novel species of the genus Nocardia, Nocardia terpenica sp. nov. is proposed for the two strains. The type strain is IFM 0706(T) (=JCM 13033(T)=DSM 44935(T)=NBRC 100888(T)).

• Nocardia coubleae sp. nov., isolated from oil-contaminated Kuwaiti soil.

  Authors: Verónica Rodríguez-Nava, Z U Khan, Gabriele Pötter, Reiner M Kroppenstedt, Patrick Boiron, Frédéric Laurent

  International journal of systematic and evolutionary microbiology. 08/2007; 57(Pt 7):1482-6.

  Two bacterial isolates from Kuwaiti soil contaminated by crude oil were analysed by using a polyphasic taxonomic approach. The isolates, designated OFN N11 and OFN N12(T), were shown to haveTwo bacterial isolates from Kuwaiti soil contaminated by crude oil were analysed by using a polyphasic taxonomic approach. The isolates, designated OFN N11 and OFN N12(T), were shown to have molecular, chemical and morphological properties typical of members of the genus Nocardia. Based on a multigenic approach that included 16S rRNA, hsp65 and sod gene sequencing, these novel isolates formed a monophyletic clade within the genus Nocardia. The closest species was Nocardia ignorata (with 99.4 %, 99.5 %, 98.6 % gene sequence similarity to the 16S rRNA, hsp65 and sod genes, respectively). The novel isolates could be distinguished phenotypically from the type strains of recognized species of the genus Nocardia. The novel isolates were not related to the type strain of N. ignorata in DNA-DNA hybridization experiments (26 % relatedness). On the basis of these genotypic and phenotypic data, the two isolates appear to represent a novel species, for which the name Nocardia coubleae sp. nov. is proposed. The type strain is OFN N12(T) (=DSM 44960(T)=CIP 108996(T)).