-
Kwang Soo Kim,
Jong-Tae Kim,
Seon-Jin Lee,
Min Ah Kang,
In Seong Choe,
Yun Hee Kang,
Seon-Young Kim,
Young Il Yeom,
Young-Ha Lee,
Joo Heon Kim,
Kyo Hyun Kim,
Chang Nam Kim, Jong Wan Kim,
Myoung-Soo Nam,
Hee Gu Lee
[show abstract]
[hide abstract]
ABSTRACT: BACKGROUND: Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) inhibits anoikis and affects the malignant phenotype of cancer cells. In this study, we analyzed CEACAM6 as a gene that is highly upregulated in colon cancer tissues, and examined the assertion that CEACAM6 might be a suitable candidate tumor marker for the diagnosis of colon cancer. METHODS: CEACAM6 gene expression in human colon tissues was performed by tissue microarray and analyzed using RT-PCR (each of normal and tumor tissue, n=40) and immunohistochemical and clinicopathological (colon cancer patients, n=143) analyses. RESULTS: CEACAM6 transcriptional and translational levels were significantly upregulated in human tumor tissues compared to non-tumor regions, and clinicopathological analysis revealed a significant correlation between CEACAM6 protein expression and Dukes' stage (p<0.001). High expression levels of CEACAM6 were significantly associated with lower overall survival (p<0.001) and shorter recurrence-free survival (p<0.001). We demonstrated that knockdown of CEACAM6 with CEACAM6-specific small interfering RNA in colorectal cancer cells attenuated invasivity (35%); conversely, the overexpression of CEACAM6 increased invasiveness. CONCLUSIONS: CEACAM6 is significantly upregulated in colon cancer tissues and is closely associated with poor prognosis, indicating that CEACAM6 might be used as a tumor biomarker and a potential therapeutic target for colon cancer.
Clinica chimica acta; international journal of clinical chemistry 09/2012; 415C:12-19. · 2.54 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: BACKGROUND: The aim of this study was to evaluate of the patterns of post-surgical relapse after mandibular setback surgery with minimal orthodontic preparation (MS-MO). MATERIALS AND METHODS: The subjects consisted of 15 patients with minimal pre-surgical orthodontic preparation (1.37 ± 1.69 months). Lateral cephalograms were taken in pre-surgical (T0), post-surgical 1 month (T1) and immediately after debonding (T2) stages. To evaluate the surgical changes (T1-T0) and the relapse (T2-T1), the linear and angular measurements were analyzed using paired t-test. Pearson's correlation coefficients of the horizontal and vertical relapses of Pog and Me to other measurements were calculated. Pog or Me in T1 were displaced rotationally on Ar-Pog or Ar-Me lines in T2 to evaluate the remaining surgical relapse except the rotational relapse from total relapse. RESULTS: The mandible relapsed anteriorly 3.53 mm (Pog) and 4.00 mm (Me) and superiorly 2.72 mm (Pog) and 2.44 mm (Me). FH to Ar-Pog and FH to Ar-Me decreased by about 2°. Pure surgical relapses at Pog and Me, except rotational relapses, were about 0.5 mm anteriorly and inferiorly 0.8 mm. CONCLUSION: The vertical relapse might induce mandibular rotation with the horizontal relapse. For an accurate prediction after MS-MO, the rotational relapse might be considered.
Journal of cranio-maxillo-facial surgery: official publication of the European Association for Cranio-Maxillo-Facial Surgery 07/2012; · 1.25 Impact Factor
-
Yun Hee Kang,
Na Young Ji,
Seung Ro Han,
Chung Il Lee,
Jae Wha Kim,
Young Il Yeom,
Young Ho Kim,
Ho Kyung Chun, Jong Wan Kim,
Jin Woong Chung,
Dong Kuk Ahn,
Hee Gu Lee,
Eun Young Song
[show abstract]
[hide abstract]
ABSTRACT: In our previous study, we reported that endothelial cell specific molecule-1 (ESM-1) was increased in tissue and serum from colorectal cancer patients and suggested that ESM-1 can be used as a potential serum marker for early detection of colorectal cancer. The aim of this study was to evaluate the role of ESM-1 as an intracellular molecule in colorectal cancer. ESM-1 expression was knocked down by small interfering RNA (siRNA) in colorectal cancer cells. Expression of ESM-1 siRNA decreased cell survival through the Akt-dependent inhibition of NF-κB/IκB pathway and an interconnected reduction in phospho-Akt, -p38, -ERK1, -RSK1, -GSK-3α/β and -HSP27, as determined by a phospho-MAPK array. ESM-1 silencing induced G(1) phase cell cycle arrest by induction of PTEN, resulting in the inhibition of cyclin D1 and inhibited cell migration and invasion of COLO205 cells. Consistently, ESM-1 overexpression in HCT-116 cells enhanced cell proliferation through the Akt-dependent activation of NF-κB pathway. In addition, ESM-1 interacted with NF-κB and activated NF-κB promoter. This study demonstrates that ESM-1 is involved in cell survival, cell cycle progression, migration, invasion and EMT during tumor invasion in colorectal cancer. Based on our results, ESM-1 may be a useful therapeutic target for colorectal cancer.
Cellular signalling 06/2012; 24(10):1940-9. · 4.09 Impact Factor
-
Yun Hee Kang,
Mi-Young Park,
Do-Young Yoon,
Seung Ro Han,
Chung Il Lee,
Na Young Ji,
Pyung-Keun Myung,
Hee Gu Lee,
Jae Wha Kim,
Young Il Yeom,
Ye Jin Jang,
Dong Kuk Ahn, Jong Wan Kim,
Eun Young Song
[show abstract]
[hide abstract]
ABSTRACT: IL-32 is a newly discovered cytokine. Recently, various reports suggest that it plays a role as a proinflammatory mediator and may be involved in several cancer carcinogenesis. However, IL-32 expression in hepatocellular carcinoma (HCC) remains unclear. In this study, we investigated the expression and role of IL-32α in hepatocellular carcinoma, because IL-32 was identified as an upregulated gene in hepatocellular carcinoma tissues compared to nontumorous regions using DNA microarray. IL-32α was overexpressed in tissue and serum from patients with HCC and localized in the cytoplasm and nucleus of hepatocellular carcinoma tumor cells. Moreover, secreted IL-32α concentration in the serum of patients with hepatocellular carcinoma was elevated as compared with those in the normal serum using a developed sandwich ELISA. Furthermore, IL-32α suppression in hepatocellular carcinoma decreased expression of phospho-p38 MAPK, NF-κB, and antiapoptotic protein Bcl-2 and induced expression of proapoptotic proteins as well as p53 and PUMA resulting in the suppression of cell growth and induction of intrinsic apoptosis. Based on our results, we suggest that IL-32α is involved in the progression of hepatocellular carcinoma and may be a useful biomarker for diagnosis and therapeutic target of hepatocellular carcinoma.
Cancer letters 12/2011; 318(2):226-33. · 4.86 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Ophthalmomyiasis rarely occurs worldwide, and has not been reported in Korea. We present here a case of ophthalmomyiasis caused by Phormia sp. fly larva in an enucleated eye of a patient. In June 2010, a 50-year-old man was admitted to Dankook University Hospital for surgical excision of a malignant melanoma located in the right auricular area. He had a clinical history of enucleation of his right eye due to squamous cell carcinoma 5 years ago. During hospitalization, foreign body sensation developed in his right eye, and close examination revealed a fly larva inside the eye, which was evacuated. The larva was proved to be Phormia sp. based on the morphology of the posterior spiracle. Subsequently, no larva was found, and the postoperative course was uneventful without any complaints of further myiasis. This is the first case of ophthalmomyiasis among the literature in Korea, and also the first myiasis case caused by Phormia sp. in Korea.
The Korean Journal of Parasitology 06/2011; 49(2):173-5. · 1.04 Impact Factor
-
Yun Hee Kang,
Na Young Ji,
Chung Il Lee,
Hee Gu Lee,
Jae Wha Kim,
Young Il Yeom,
Dae Ghon Kim,
Seung Kew Yoon, Jong Wan Kim,
Pil Je Park,
Eun Young Song
[show abstract]
[hide abstract]
ABSTRACT: Endothelial cell-specific molecule-1 (ESM-1) is a secretory proteoglycan comprising a mature polypeptide of 165 amino acids and a single dermatan sulfate. The aim of this study was to evaluate endothelial cell-specific molecule-1 (ESM-1) as a hepatocellular carcinoma (HCC) marker and to analyze the effect of ESM-1 gene silencing in hepatocellular carcinoma cells. RT-PCR and Western Blot analysis revealed overexpression of ESM-1 in human HCC liver tissue and in serum from patients with HCC. Sandwich ELISA assay was used for quantitative analysis of ESM-1 in serum. Levels of ESM-1 were significantly elevated in the serum of patients with HCC (n = 40) as compared to serum from patients with hepatitis (AH, n = 40; CH, n = 39) or liver cirrhosis (n = 40) or from healthy subjects (n = 40). The accuracy of ESM-1 for HCC was higher than that of α-fetoprotein (AFP) according to ROC curve analysis. Expression of ESM-1 siRNA decreased cell survival through the inhibition of NF-κB pathway and induced cell cycle arrest by PTEN induction resulting in the inhibition of cyclin D1 in SK-Hep1 cells. Furthermore, ESM-1 silencing inhibited cell migration and invasion of SK-Hep1 cells. This study demonstrates that ESM-1 as a potential tumor marker is overexpressed in most tissues and serum in the presence of HCC and is involved with cell survival, cell cycle progression, migration, and invasion of hepatocellular carcinoma cells. Based on our results, we suggest that ESM-1 or a combination of ESM-1 and AFP is useful markers for diagnosis of HCC and ESM-1 may be useful therapeutic target of hepatocellular carcinoma.
Amino Acids 03/2011; 40(3):1003-13. · 3.25 Impact Factor
-
Animal Cells and Systems. 03/2011; 15(1):29-36.
-
[show abstract]
[hide abstract]
ABSTRACT: This study was carried out to evaluate the vertical and horizontal changes after maxillary surgery using resorbable plates made of an l-lactic acid and d-lactic acid copolymer of PLA (P(L/DL)LA), and to analyze factors of skeletal pattern and surgical movement affecting the post-surgical change.
The subjects consisted of 20 patients who underwent bimaxillary surgery using P(L/DL)LA resorbable plate (Inion CPS, Inion Ltd., Tampere, Finland). Lateral cephalograms were taken at 1 month before (T0), 1 month after (T1) and 6 months after surgery (T2). They were divided into two groups, one showing less relapse (LR) and one more relapse (MR) using K-means clustering analysis of post-surgical relapse (T2-T1). In terms of linear and angular variables of maxillary relapse, the two groups were compared with measurements at the pre-surgical stage (T0), the surgical change obtained (T1-T0) and post-surgical relapse (T2-T1) using Mann-Whitney test.
Because Frankfort horizontal (FH) to anterior nasal spine (ANS) showed the largest variance in post-surgical relapse of the maxilla, it was used for clustering analysis to define the MR and LR groups. Comparison between the MR and LR groups showed that a short preoperative anterior facial height (AFH) was a significant factor in maxillary relapse. The AFH of the MR group was significantly shorter pre-surgically (p<0.01) and was significantly increased postoperatively (p<0.05) when compared to the LR group. In post-surgical relapse, AFH, FH to A, FH to ANS and the palatal plane angle of the MR group were significantly decreased.
In the case of a short AFH, P(L/DL)LA resorbable plates may not provide enough vertical stability in the maxillary position due to the muscle force. Facial height might be an important factor when deciding to use P(L/DL)LA resorbable plates for maxillary surgery.
Journal of cranio-maxillo-facial surgery: official publication of the European Association for Cranio-Maxillo-Facial Surgery 02/2011; 39(8):578-82. · 1.25 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: A rationally designed, coumarin-based fluorescent sensor imino-coumarin (IC) displays high selectivity for Cu(2+) over a variety of competing metal ions in aqueous solution with a significant fluorescence increase. DFT/TDDFT calculations support that the fluorescence 'turn-on' of IC originates from blocking the electron transfer of the nitrogen lone pair upon complexation with Cu(2+). IC was successfully applied to microscopic imaging for detection of Cu(2+) in LLC-MK2 cells (in vitro) and several living organs (in vivo).
Chemical Communications 01/2011; 47(11):3165-7. · 6.17 Impact Factor
-
Heejung Kim,
Seok Hoon Jeong,
Kyoung Ho Roh,
Seong Geun Hong, Jong Wan Kim,
Myung-Geun Shin,
Mi-Na Kim,
Hee Bong Shin,
Young Uh,
Hyukmin Lee,
Kyungwon Lee
[show abstract]
[hide abstract]
ABSTRACT: Clostridium difficile is a major cause of antibiotic-associated diarrhea. The objective of this study was to characterize clinical isolates of C. difficile obtained from various regions in Korea with regard to their toxin status, molecular type, and antimicrobial susceptibility.
We analyzed a total of 408 C. difficile isolates obtained between 2006 and 2008 from 408 patients with diarrhea in 12 South Korean teaching hospitals. C. difficile toxin genes tcdA, tcdB, cdtA, and cdtB were detected by PCR. Molecular genotyping was performed by PCR ribotyping. Antimicrobial susceptibilities of the 120 C. difficile isolates were assessed by agar dilution methods.
Among 337 toxigenic isolates, 105 were toxin A-negative and toxin B-positive (A(-)B(+)) and 29 were binary toxin-producing strains. PCR ribotyping showed 50 different ribotype patterns. The 5 most frequently occurring ribotypes comprised 62.0% of all identified ribotypes. No isolate was susceptible to cefoxitin, and all except 1 were susceptible to piperacillin and piperacillin-tazobactam. The resistance rates of isolates to imipenem, cefotetan, moxifloxacin, ampicillin, and clindamycin were 25%, 34%, 42%, 51%, and 60%, respectively. The isolates showed no resistance to metronidazole or vancomycin.
This is the first nationwide study on the toxin status, including PCR ribotyping and antimicrobial resistance, of C. difficile isolates in Korea. The prevalence of A-B+ strains was 25.7%, much higher than that reported from other countries. Binary toxin-producing strains accounted for 7.1% of all strains, which was not rare in Korea. The most prevalent ribotype was ribotype 017, and all A-B+ strains showed this pattern. We did not isolate strains with decreased susceptibility to metronidazole or vancomycin.
The Korean Journal of Laboratory Medicine 10/2010; 30(5):491-7. · 0.63 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Serum cystatin B (CSTB) concentrations have been reported to be increased in patients with hepatocellular carcinoma compared to concentrations seen in normal subjects. In this study, we developed a "fluorescent microsphere immunoassay" (FMI) capable of specifically detecting CSTB in serum.
The FMI used a microparticle conjugated polyclonal antibody to CSTB and biotinylated monoclonal antibody as capture protein and probe protein, respectively. The results were obtained using the Bio-Plex(200) system.
The dose-response relationship between CSTB and fluorescent intensity showed linearity in the range 0-1000 pg/mL and 7 pg/mL, sensitivity lower than 11.2 pg/mL. This result revealed that the FMI system was more sensitive than enzyme-linked immunoassay (ELISA). Additionally, the FMI system used smaller sample volumes compared to ELISA.
We measured CSTB with both the FMI and an ELISA procedure and compared the two methods. The CSTB concentrations in serum specimens as measured with the FMI assay system were similar to those measured with ELISA. Thus, the new FMI using the Bio-Plex system may be useful for detection of CSTB in human serum.
Clinical Chemistry and Laboratory Medicine 10/2010; 49(1):151-5. · 2.15 Impact Factor
-
Na Young Ji,
Young-Ho Kim,
Ye Jin Jang,
Yun Hee Kang,
Chung Il Lee,
Jae Wha Kim,
Young Il Yeom,
Ho Kyung Chun,
Yoon-Ho Choi,
Joo Heon Kim, Jong Wan Kim,
Hee Gu Lee,
Eun Young Song
[show abstract]
[hide abstract]
ABSTRACT: No ideal serum markers for screening colorectal cancer (CRC) have been identified. The aim of this study was to determine the usefulness of endothelial cell-specific molecule-1 (ESM-1) as a serum marker for CRC. Illumina microarray was carried out to search CRC-related biomarkers. cDNA microarray detected that ESM-1 was one of the overexpressed genes in CRC. Overexpression of ESM-1 mRNA was confirmed in tissues of CRC by RT-PCR and real-time PCR. Immunohistochemical staining showed strong expression of ESM-1 in the cytoplasm of tumor cells. Overexpression of ESM-1 in human serum with CRC was found by Western blot analysis. For quantitative analysis of ESM-1 in serum, we determined the ESM-1 levels in serum specimens using an ELISA kit. We showed that the ESM-1 levels in the serum of patients with CRC were significantly elevated (70.1 ± 29.7 pg/mL) compared to healthy subjects (29.7 ± 14.9 pg/mL). The accuracy, sensitivity, and specificity of ESM-1 for CRC were 0.94, 99%, and 73%, respectively, by receiver operating characteristics curve analysis. The positive predictive value and negative predictive value were 63% and 95%, respectively. The likelihood ratios of a positive or negative test result were 73 and 0.27, respectively. When analyzed with a Cox regression model, a higher serum ESM-1 level (≥76.0 pg/mL) was correlated with poor prognosis. This study suggests that expression of ESM-1 is increased in tissue and serum of CRC patients and that ESM-1 can be used as a potential serum marker for the early detection of CRC.
Cancer Science 10/2010; 101(10):2248-53. · 3.33 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: The purpose of this study was to use lateral cephalometry and computed tomography (CT) to examine the volumetric, planar, and linear changes in the pharyngeal airway after mandibular setback surgery.
The pharyngeal airways of 12 subjects who underwent mandibular setback surgery at Seoul National University Dental Hospital were assessed linearly and volumetrically on lateral cephalometric radiographs and CT before surgery and 6 months after surgery. The pharynx, nasopharynx, and oropharynx were evaluated by volumetric analysis. Pharyngeal depth, airway space, pharyngeal soft-tissue thickness, and hyoid bone position were measured by linear analysis. The axial section area of the airway was measured by area analysis.
From the linear analysis, a significant decrease in pharyngeal depth and a significant posterior movement of the hyoid bone (P <0.05) were noted. Volumetric analysis by CT showed that the oropharynx decreased after mandibular setback surgery. However, the volume and the axial section area of the airway in the CT images did not change significantly after mandibular setback surgery (P >0.05).
Although the structures around the mandible inevitably moved backward after mandibular setback surgery on linear analysis, physiologic deformation could occur to preserve the airway capacity after sagittal compression.
American journal of orthodontics and dentofacial orthopedics: official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics 09/2010; 138(3):292-9. · 1.33 Impact Factor
-
Journal of clinical orthodontics: JCO 05/2010; 44(5):313-8.
-
[show abstract]
[hide abstract]
ABSTRACT: To determine the histological reaction of the root and bone as a mini-implant approaches the root.
Two kinds of mini-implants were inserted into the buccal alveolar bone of 4 beagles (2 males and 2 females). The specimens were classified as the near-root group, the PDL contact group, the root contact group, and the root perforation group. Cementum resorption, dentin resorption, cementum repair, cementum growth, ankylosis, root cracking, and root fracture were assessed as the implant neared the root.
The incidence of root resorption increased when the mini-implant was less than 0.6 mm from the root in the near-root group and PDL contact group. Root cracking and root fracture occurred in the root contact group and root perforation group. Bone resorption and ankylosis were observed in some specimens. However, some specimens of the PDL contact group and root contact group had cementum growth or little root resorption despite proximity to the root. In the root perforation group, root resorption and ankylosis occurred on the side opposite the insertion.
There is a risk of root contact and severe tissue damage from a thick mini-implant and the drilling procedure, either of which can induce root resorption or ankylosis. Use of smaller mini-implants may reduce root contact and tissue damage. However, the small mini-implant may need enhancement of its stability.
The Angle Orthodontist 05/2010; 80(3):452-8. · 1.21 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: On July 2009, 5 fly larvae were discovered inside the nose of a 76-year-old female. She was living in Cheonan-si, and in a state of coma due to rupture of an aortic aneurysm. Surgery was performed on the day of admission, and the larvae were found 4 days later. By observing their posterior spiracle, the larvae were identified as Lucilia sericata. Considering the rapid development of this species, the infection was likely acquired during hospitalization. Further investigation on the hospital environment should be needed to know the origin of the infection.
The Korean Journal of Parasitology 12/2009; 47(4):405-7. · 1.04 Impact Factor
-
Na Young Ji,
Mi-Young Park,
Yun Hee Kang,
Chung Il Lee,
Dae Ghon Kim,
Young Il Yeom,
Ye Jin Jang,
Pyung-Keun Myung, Jong Wan Kim,
Hee Gu Lee,
Jae Wha Kim,
Kyeong Lee,
Eun Young Song
[show abstract]
[hide abstract]
ABSTRACT: Annexin II (Annexin A2, ANXA2) is a 36 kDa calcium-dependent phospholipid-binding protein that is located on the surface of most eukaryotic cells. ANXA2 is involved in several biological processes, including anti-inflammatory effects, Ca27+-dependent exocytosis, immune responses, Ca2+ transport and phospholipase A2 regulation. In our previous study, ANXA2 was identified as an up-regulated gene in hepatocellular carcinoma (HCC) tissue by cDNA microarray. In the present study, we have evaluated ANXA2 as a tumor-associated marker of HCC. We determined the ANXA2 levels in human liver tissues with HCC using real-time RT-PCR and Western blot analysis. For quantitative analysis of the ANXA2 protein in body fluids, we developed a sandwich ELISA system in which a polyclonal antibody and a monoclonal antibody specific to ANXA2 were employed as a capture antibody and a probe antibody, respectively. We detected the ANXA2 concentration in human serum using our newly developed system and evaluated its usefulness as a tumor marker. Overexpression of ANXA2 in human liver tissue was confirmed by real-time RT-PCR and Western blot analysis. The sandwich ELISA system for ANXA2 was developed for the detection of ANXA2 in human samples. The dose-response relationship between ANXA2 and optical density was linear in the range of 0-10 microg/ml and the sensitivity was 0.02 microg/ml. We determined the ANXA2 concentration in serum specimens using the newly developed sandwich ELISA. The serum ANXA2 concentrations of the patients with HCC (53.38+/-36.23 microg/ml) were significantly elevated when compared with those of normal individuals (28.81+/-24.94 microg/ml). These results suggest that expression of ANXA2 may be increased in HCC patients and may play an important role in liver cancer progression. This new ELISA method can be used as a tool for the detection of ANXA2 in human serum, particularly for cancer diagnostics.
International Journal of Molecular Medicine 12/2009; 24(6):765-71. · 1.98 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To analyze the mechanical effects of the length and the various shapes such as cylindrical shape, taper shape, and dual-thread shape on the insertion and removal torque of mini-implants.
Mini-implants (diameter 1.6 mm and length 6 mm and 8 mm) consisting of cylindrical, taper, and dual-thread groups were inserted and removed in Sawbones while measuring the torque and time. Mechanical analysis was done of maximum insertion torque (MIT), maximum removal torque (MRT), torque ratio (TR; MRT/MIT), insertion angular momentum (IAM), removal angular momentum (RAM), and time of MIT. Measurements were statistically evaluated to analyze any differences of shapes and lengths.
The cylindrical shape had the lowest MIT and MRT in each length. Although taper shape showed the highest MIT in each length, dual-thread shape showed significantly higher MRT, TR, and RAM in each length (P < .05). Dual-thread groups showed a gentle increase of insertion torque and a gentle decrease of removal torque in contrast to the other shape groups. However, it had higher IAM and time of MIT. The long length group showed significantly higher measurements except for TR.
Dual-thread shape provided better mechanical stability with high removal torque on the broad range than other shapes. However, dual-thread shape may need improvement for reducing the long insertion time to decrease the stress to the surrounding tissue.
The Angle Orthodontist 10/2009; 79(5):908-14. · 1.21 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: This clinical research was carried out to investigate the influences of orthognathic surgery on the temporomandibular joint (TMJ) using magnetic resonance imaging (MRI) taken before and after the surgery.
There were 23 subjects who had a bilateral sagittal split ramus osteotomy in Seoul National University Bundang Hospital from June 2004 to December 2006 and their condyles. The mean age of patients was 22.09 +/- 3.65 and the number of men and women was 10 and 13, respectively. Bilateral sagittal planes of 46 condyles had taken MRI in both closed and open mouth states before and 3 months after surgery. The differences between pre- and postoperative disc positions and internal disc derangement (IDD) stages were evaluated. Furthermore, a correlation analysis of the amounts of mandibular set-back and disc positions was carried out. Paired-samples of the T tests, the McNemar-Bowker test and Pearson correlation analysis were applied as statistical methods. In this study, a significance level is considered to be less than 0.05.
The disc positions had not shown statistically significant differences between pre- and post-operation in the closed state. However there were significant increases of distances between the discs and condyle on mouth opening. For instance, 0.70 +/- 1.30 mm was an average (P < .01). The derangement stages of each condyle were not changed through the operation. Although there were improved or aggravated cases, the orthognathic surgery had no influence on derangement stages in this study. Last, there was no significant correlation between the 2 variables of the amounts of set-back and the changes of disc position.
Although there were significant differences of the disc positions in the open mouth state, the orthognathic surgery appears not to have had an effect on the TMJ.
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 06/2009; 108(1):15-21. · 1.50 Impact Factor
-
Hyo Sung Jung,
Pil Seung Kwon,
Jeong Won Lee,
Jae Il Kim,
Chang Seop Hong, Jong Wan Kim,
Shihai Yan,
Jin Yong Lee,
Jung Hwa Lee,
Taiha Joo,
Jong Seung Kim
[show abstract]
[hide abstract]
ABSTRACT: A novel coumarin-based fluorogenic probe bearing the 2-picolyl unit (1) was developed as a fluorescent chemosensor with high selectivity and suitable affinity in biological systems toward Cu(2+) over other cations tested. The fluorescence on-off mechanism was studied by femtosecond time-resolved fluorescence (TRF) upconversion technique and ab initio calculations. The receptor can be applied to the monitoring of Cu(2+) ion in aqueous solution with a pH span 4-10. To confirm the suitability of 1 for biological applications, we also employed it for the fluorescence detection of the changes of intracellular Cu(2+) in cultured cells. The results indicate that 1 should be useful for the fluorescence microscopic imaging and the study on the biological functions of Cu(2+).
Journal of the American Chemical Society 03/2009; 131(5):2008-12. · 9.91 Impact Factor
