L Weglarz

Medical University of Silesia in Katowice, Catowice, Silesian Voivodeship, Poland

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Publications (16)16.67 Total impact

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    ABSTRACT: The effect of an excessive inorganic silicon oral intake on the activity of basic antioxidant enzymes was studied in rats. Activities of superoxide dismutase, catalase, and glutathione peroxidase were measured in liver and kidney tissues of animals receiving per os sodium metasilicate nonahydrate (Na2SiO3.9H2O) (Sigma, [St. Louis, MO]) dissolved in their drinking water. A decrease of the activity of all the studied enzymes was found in the samples derived from the experimental group. The results obtained indicate the free oxygen radicals participation in the potential pathologic events in the conditions of systemic hypersilicemia.
    Biological Trace Element Research 08/1994; 42(1):63-70. DOI:10.1007/BF02990489 · 1.75 Impact Factor
  • L Weglarz · J Gmiński · M Drózdz · M Goss
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    ABSTRACT: The effect of elastin peptides (kappa-elastin) was investigated on murine fibroblasts. The data indicate that elastin peptides increase the activities of antioxidant enzymes detoxifying free radicals and increase the lipid peroxide concentration within the cell. These results suggest that the influence of elastin peptides on oxygen metabolism may be related to their activities in vivo following elastin degradation and can contribute to their role in the pathogenesis of atherosclerosis.
    Cytobios 02/1992; 69(277):87-90.
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    ABSTRACT: Elastin-derived peptides, kappa-elastin, prepared by chemical degradation of insoluble elastin from bovine ligamentum nuchae, were shown to increase the elastase-like activity in the culture medium and cell fractions in fibroblasts. Preincubation of cells with nifedipine (calcium channel blocker) and trifluoperazine (calmodulin antagonist) induced a decrease in the activities of the enzyme under study. These data suggest the possibility of pharmacological modulation of the biological effects induced by elastin-derived peptides.
    Biochemical Medicine and Metabolic Biology 05/1991; 45(2):254-7. DOI:10.1016/0885-4505(91)90028-J
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    ABSTRACT: 1. Elastin peptides (kappa-elastin) prepared by alcoholic potassium hydroxide degradation of insoluble elastin were shown to increase the activities of antioxidant enzymes (SOD, CAT, GSH-Px) and the lipid peroxide concentration within fibroblasts. 2. The preincubation of cells with nifedipine (calcium channel antagonist) and trifluoperazine (calmodulin antagonist) caused the decrease in the activities of studied enzymes and the concentration of TBA-reactive products in fibroblasts stimulated with kappa-elastin. 3. The preincubation with ketotifen (antiallergic drug) has no effect on the activities of SOD, CAT, GSH-Px and the lipid peroxide concentration in stimulated cells. 4. These data suggest the possibilities of pharmacological modulation of the biological effects induced by elastin-derived peptides.
    General Pharmacology 02/1991; 22(3):495-7. DOI:10.1016/0306-3623(91)90012-U
  • L Weglarz · G Bartosz
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    ABSTRACT: The effect of hydralazine on the oxygen free radical production was studied in whole cultured murine liver fibroblasts and mitochondrial and microsomal fractions of the cells by ESR spin trapping with DMPO and measurement of Tiron semiquinone formation. Hydralazine itself was found to generate free radicals in phosphate buffer and especially in Eagle's Minimal Essential Medium. Most of the adduct of the spin trap DMPO was due to its reaction with hydralazine-induced hydroxyl radical. Moreover, this compound stimulated free radical formation in fibroblasts. These data suggest that hydralazine alters the cellular free radical metabolism which may have implications for the biological activity of this drug.
    Free Radical Biology and Medicine 02/1991; 11(2):149-55. DOI:10.1016/0891-5849(91)90165-Y · 5.74 Impact Factor
  • L Weglarz · G Bartosz
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    ABSTRACT: 1. Incubation with hydralazine was shown to induce degradative changes of calf thymus DNA spin-labeled with 3-(2-bromoacetamido)-2,2,5,5-tetramethyl-1-pyrrolidono-1-oxyl and 4-(2-bromoacetamido)-2,2,6,6-tetramethylpiperidino-1-oxyl detectable from electron spin resonance specta. 2. Hydralazine, especially in the presence of Fe2+ induced formation of thiobarbituric acid (TBA)-reactive DNA degradation products. 3. The formation of TBA-reactive products was prevented by catalase, EDTA and scavengers of .OH radicals and enhanced by superoxide dismutase which suggests that .OH radicals formed by the Fenton mechanism mediate the DNA damage by hydralazine-Fe2+.
    International Journal of Biochemistry 02/1991; 23(7-8):663-7. · 4.24 Impact Factor
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    ABSTRACT: Cattle tissues (liver, kidney, brain, and lung) that had been polluted with heavy metals were tested for their ability to alter fibroblast culture growth, cellular protein and DNA content, and fibroblast DNA synthesis. At 72 hr of incubation a significant increase in cellular DNA and [14C]thymidine incorporation was noted in the primary cultures as well as in the subcultures compared to controls. Fibroblast cultures also displayed growth inhibition and reduction in protein content. The measurement of basic biochemical parameters of the fibroblast culture may represent a sensitive means of assessing rapidly the activity of heavy metals deposited in the tissues of cattle as a result of their grazing on polluted soil.
    Environmental Research 05/1990; 51(2):163-9. DOI:10.1016/S0013-9351(05)80086-6 · 4.37 Impact Factor
  • L Weglarz · M Drózdz · M Wardas
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    ABSTRACT: Murine fibroblasts cultured in vitro were treated with hydrazinophthalazine drugs inducing the lupus erythematosus-like syndrome. Collagenase enzyme activity in the growth medium was found to be increased 3-fold by hydralazine and 1.8-fold by binazine, as compared to the control cultures. The enhanced level of collagenolytic activity points to the metabolic changes in the connective tissue in response to hydrazinophtalazines.
    Biomedica biochimica acta 02/1990; 49(4):289-91.
  • L Weglarz · M Drózdz · M Goss
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    ABSTRACT: 1. Four groups of rats were injected with four different anti-inflammatory drugs (indomethacin, phenylbutazone, acetylsalicylic acid and hydrocortisone) and the activities of superoxide dismutase, catalase and glutathione peroxidase were studied in the liver and kidney. 2. The drugs treatment resulted in decreased activity of the enzymes in both organs compared to the control animals. 3. In vivo tissue peroxidation was also effected by the drugs used. 4. Our results indicate that the changes of oxygen free-radical metabolism contribute to the action of these drugs in vivo.
    Comparative Biochemistry and Physiology Part C Comparative Pharmacology and Toxicology 02/1990; 96(1):83-5. DOI:10.1016/0742-8413(90)90048-E
    Comparative Biochemistry and Physiology Part C Comparative Pharmacology and Toxicology 01/1990; 96(1):83-85.
  • L Weglarz · M Drózdz · M Wardas · M Goss
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    ABSTRACT: Variations in superoxide dismutase, catalase and glutathione peroxidase activity were studied in fibroblasts cultured in the presence of hydralazine, a drug known to be an inducer of the so-called collagen-like syndrome. The results demonstrated that both superoxide dismutase and catalase undergo a marked decrease in their activity, whereas glutathione peroxidase manifests a significant increase in its activity after treatment with hydralazine as compared to control cell cultures. Also the lipid peroxide concentration as expressed by the malondialdehyde amount was estimated in the above cultures. The altered antioxidant enzyme activity and the presence of byproducts of free radical damage support the possibility that the action of hydralazine leading to the pathogenesis of collagen disease-like syndrome involves an abnormal free-radical metabolism.
    Biomedica biochimica acta 02/1989; 48(8):489-93.
  • M Drózdz · M Wardas · L Weglarz · B Kula
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    ABSTRACT: The aim of this study was to characterize glycosaminoglycan components in human palatal tonsils and to determine whether the proportions of individual glycosaminoglycans change with bacterial infection of the palatal tonsils and after recovery. The changes in glycosaminoglycans' content in the course of tonsillitis were mainly related to heparin and chondroitin-4-sulphate (increase) and hyaluronic acid (decrease). After recovery the polydispersity of glycosaminoglycans was found to be comparable to that in normal palatal tonsils.
    Acta medica Polona 02/1989; 30(3-4):141-5.
  • L Weglarz · M Drózdz
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    ABSTRACT: The paper presents recent data concerning the effect of growth factors on the metabolism of collagen, collagenases and glycosaminoglycans in vitro. The hypotheses of the mitogenic action of peptide factors and their role in the control of synthesis and breakdown of polyphosphoinositides are also reviewed.
    Postȩpy higieny i medycyny doświadczalnej 02/1989; 43(2-4):255-74. · 0.57 Impact Factor
  • M Drózdz · L Weglarz · M Wardas · B Kula
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    ABSTRACT: Fibroblast cultures exposed to the drugs inducing a collagen-like syndrome (hydralazine and binazine) displayed growth inhibition and decrease in cellular protein content in a dose-dependent manner compared with control cultures. This was accompanied by the inhibitory effect of the drugs on DNA synthesis. The changes in the basic biochemical parameters of fibroblasts testify to the toxicity of hydrazinophthalazines in the connective tissue.
    Clinical physiology and biochemistry 02/1989; 7(2):97-100.
  • L Weglarz · M Wardas · M Drózdz · B Kula
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    ABSTRACT: The trials have been undertaken to elaborate the experimental conditions of granular cells cultivation for FSH activity testing.
    Experimental and clinical endocrinology 10/1988; 92(1):115-8. DOI:10.1055/s-0029-1210790
  • M Drózdz · B Kula · M Wardas · L Weglarz
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    ABSTRACT: A drug-induced collagen-like syndrome was produced in rats by hydralazine administration for 8, 9 and 10 months. The hyaluronic acid content and the hyaluronidase activity in liver and spleen were studied. A decrease in the hyaluronic acid amount was accompanied by an enhanced activity of hyaluronidase in both tissues. The results provide evidence for metabolic disturbances of glycosaminoglycans in the connective tissue in the collagen disease-like syndrome.
    Biomedica biochimica acta 02/1988; 47(3):247-50.