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ABSTRACT: An ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC/Q-TOF-MS) method coupled with a principal component analysis (PCA) was developed and applied toward identifying donkey-hide gelatin, bovine-hide gelatin, pig-hide gelatin, tortoise shell glue, and deerhorn glue. The UPLC-MS data of the trypsin digested samples were subjected to principal component analysis (PCA) in order to classify these five gelatins. Additionally, marker peptides given by the loadings plot of PCA were identified based on a comparison of recorded LC-MS data with a previously reported database of the corresponding gelatin variants. The results from this study indicate that the proposed method is reliable, and it has been successfully applied to the identification of variants of gelatins commonly used in Traditional Chinese Medicine (TCM).
Journal of pharmaceutical and biomedical analysis 03/2012; 62:191-5. · 2.45 Impact Factor
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ABSTRACT: The phenomenon known as "matrix-induced enhancement effect" is not only observed in the analysis of pesticides in food, but also in Chinese herbs. Several approaches have been proposed to overcome the matrix-induced effect, but each method has serious limitations. Compared with standard calibration methods, the procedure with adding analyte protectants offers a more convenient and effective route to solve the problem. In the current study, we have analyzed 195 types of pesticides in Chinese herbs by gas chromatography-mass spectrometry (GC-MS), and the compounds that are susceptible to matrix effect were picked up and confirmed. In addition, several analyte protectants were evaluated and the most effective combination was determined. D-ribonic acid-γ-lactone (2 mg/ml) and D-sorbitol (1 mg/ml) were shown to be the best analyte protectants for the analysis of most pesticides.
Journal of chromatography. A 01/2011; 1218(2):334-42. · 4.19 Impact Factor
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ABSTRACT: Phytochemical investigation of the dried aerial parts of Mikania micrantha led to the isolation of a new sesquiterpene, 3beta-acetoxy-1,10-epoxy-4-germacrene-12,8;15,6-diolide (1), along with six known constituents: 1,10-epoxy-4-germacrene-12,8;15,6-diolide (2), dihydromikanolide (3), potassium mikanin 3-sulfate (4), mikanin (5), alpinetin (6), and ergosta-7,22-dien-3beta-ol (7). Their structures were elucidated by spectroscopic methods, and the molecular structures and stereochemistry of sesquiterpene lactones 1-3 were revealed by single-crystal X-ray analysis. Compound 2 showed moderate activity against respiratory syncytial virus (IC(50) = 37.4 uM) and parainfluenza type 3 virus (IC(50) = 37.4 uM) with a therapeutic index (TI) of 16.0 for both compounds. Compound 4, the main component of M. micrantha, exhibited inhibitory activity against parainfluenza type 3 virus with IC(50) (19.7 uM) and TI (24.0) values comparable to those of ribavirin, serving as a positive control.
Journal of Natural Products 04/2009; 72(5):925-8. · 3.13 Impact Factor
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ABSTRACT: Rhubarb root (Dahuang) is often included as an ingredient in traditional Chinese compound prescriptions for the treatment of inflammatory diseases. This application may possibly be mediated through anti-angiogensis and thus would shed light on its potential value in cancer therapy.
To elucidate the anti-angiogenic properties of rhubarb root, we tested the inhibitory effects of different fractions and a series of anthraquinone derivatives against vessel formation in zebrafish embryos.
The 95% ethanol extract and four subsequent fractions (n-hexane, ethyl acetate, n-butanol and aqueous fractions) of rhubarb root and five anthraquinone derivatives were investigated on zebrafish model by quantitative endogenous alkaline phosphatase assay and staining assay.
Ethyl acetate fraction showed the strongest inhibition of vessel formation by 52%. Three anthraquinones (aloe-emodin, emodin and rhein) displayed potent anti-angiogenic activities.
The angiogenic properties of rhubarb root may partly account for its use in inflammatory diseases. The anthraquinones with acidic or polar, hydrophilic substitution at C-6 or C-3 positions played a substantial role in inhibiting angiogenesis. The value of the zebrafish angiogenic model is further supported.
Journal of Ethnopharmacology 12/2008; 121(2):313-7. · 3.01 Impact Factor
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ABSTRACT: Two new unusual natural pigments were first isolated from the whole herbs of Selaginella tamariscina. The structure of selaginellin A (1) was established as (R,S)-4-[(4'-hydroxy-3-((4-hydroxyphenyl)ethynyl)biphenyl-2-yl)(4-hydroxyphenyl)methylene]-2,5-cyclohexadien-1-one and selaginellin B (2) as (R,S)-4-[(4'-methoxy-4-(methyl)-3-((4-methoxyphenyl)ethynyl)biphenyl-2-yl)(4-methoxyphenyl)methylene]-2,5-cyclohexadien-1-one, along with four known biflavonoids, amentoflavone (3), hinokiflavone (4), heveaflavone (5), and 7''-O-methylamentoflavone (6). Their chemical structures were elucidated by spectral analysis of electrospray ionization mass spectroscopy (ESI-MS), one-dimensional nuclear magnetic resonance spectroscopy (1D-NMR) and two-dimensional-nuclear magnetic resonance spectroscopy (2D-NMR) including (1)H-NMR, (13)C-NMR, distortionless enhancement by polarization transfer (DEPT) and heteronuclear multiple bond coherence (HMBC), and single-crystal X-ray diffraction techniques.
CHEMICAL & PHARMACEUTICAL BULLETIN 08/2008; 56(7):982-4. · 1.59 Impact Factor
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ABSTRACT: A new sesquiterpene, neolindenenonelactone (1), was first isolated from the dried roots of Lindera aggregata (SIMS) KOSTERM., along with four known compounds, linderane (2), isolinderalactone (3), linderalactone (4), and 8-hydroxylindestenolide (5). Their chemical structures were elucidated using spectral analysis of fast atom bombardment mass spectroscopy, one-dimensional nuclear magnetic resonance spectroscopy and two-dimensional-nuclear magnetic resonance spectroscopy including (1)H-NMR, (13)C-NMR, distortionless enhancement by polarization transfer, heteronuclear multiple-bond coherence, and single-crystal X-ray diffraction techniques.
CHEMICAL & PHARMACEUTICAL BULLETIN 10/2007; 55(9):1390-2. · 1.59 Impact Factor
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ABSTRACT: Two new triterpenoid saponins, hederagenin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-arabinopyranoside named akeboside La (compound 1), oleanolic acid 3-O-α-L-arabinopyranosyl-(1→2)-(β-D-glucopyranoside named akeboside Lb (compound 2), along with five known saponins, oleanolic acid 3-O-α-L-rhamnopyranosyl-(1→2)-ot-L-arabinopyranoside (compound 3), hederagenin 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside (compound 4), oleanolic acid 3-O-β-D-xylopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside (compound 5), 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopyranosyl-(1→4)-α-D-glucopyranosyl-(1→6)-(β-D-glucopyranoside (compound 6), 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl hederagenin 28-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-(β-D-glucopyranoside (compound 7) were isolated from the n-butanol part of the 80% ethanol extracts of the dried stems of Akebia quinata (Thunb.) Decne. Compound 5 was isolated from plants of genus Akebia for the first time. The structures were elucidated on the basis of physicochemical properties and spectral data.
Journal of Integrative Plant Biology 02/2007; 49(2):196 - 201. · 2.53 Impact Factor
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ABSTRACT: Phytochemical study of the ethanol extract of the seeds of Aesculus chinensis led to the isolation of a new triterpenoid saponin (6), together with five known triterpenoid saponins (1-5). The structure of the new compound was elucidated on the basis of spectral data to be 21,28-di-O-acetylprotoaescigenin-3-O-[beta-D-glucopyranosyl(1-2)][beta-D-glucopyranosyl(1-4)]-beta-D-glucopyranosiduronic acid (aesculiside A, 6). The antiinflammatory activities of the four main saponins (1-4) were compared with those of total saponin extracts, and single saponins showed more potent activity than total saponin extracts in mice.
CHEMICAL & PHARMACEUTICAL BULLETIN 11/2004; 52(10):1246-8. · 1.59 Impact Factor
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ABSTRACT: A bioassay-guided fractionation of an ethanol extract of the seeds of Aesculus chinensis led to the isolation of two new flavanoids (1 and 2), along with eight known ones (3-10). The structures of the new compounds were elucidated by spectroscopic methods including 2D NMR. All compounds were tested for antiviral activity against respiratory syncytial virus (RSV), parainfluenza virus type 3 (PIV 3), and influenza virus type A (Flu A). Compounds 1, 2, and 6 showed significant antiviral activities against RSV with IC(50) values of 4.5, 6.7, and 4.1 microg/mL and selective index (SI) values of 15.8, 32, and 63.8, respectively. Compound 8 demonstrated significant antiviral activity against Flu A with an IC(50) of 24.5 microg/mL and a SI of 16.0, respectively.
Journal of Natural Products 05/2004; 67(4):650-3. · 3.13 Impact Factor
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ABSTRACT: Five compounds have been isolated from the dried whole plants of Selaginella uncinata, two of them were new chromone glycosides, 5-hydroxy-2,6,8-trimethylchromone 7-O-beta-D-glucopyranoside (uncinoside A) and 5-acetoxyl-2,6,8-trimethylchromone 7-O-beta-D-glucopyranoside (uncinoside B). Their structures were elucidated by spectroscopic methods including one- and two-dimensional NMR techniques. The other three compounds were identified as 8-methyl eugenitol, amentoflavone and hinokiflavone. Uncinoside A and B showed potent antiviral activities against respiratory syncytial virus (RSV) with IC(50) value of 6.9 and 1.3 microg/ml, moderate antiviral activities against parainfluenza type 3 virus (PIV 3) with IC(50) value of 13.8 and 20.8 microg/ml, respectively.
CHEMICAL & PHARMACEUTICAL BULLETIN 12/2003; 51(11):1264-7. · 1.59 Impact Factor
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ABSTRACT: Phytochemical investigation of the ethanol extract of the leaves of Ligustrum robustum, monitored by a bioassay involving the hemolysis of red blood cells induced by 2,2'-azo-bis(2-amidinopropane) dihydrochloride, led to the isolation of three new glycosides, ligurobustosides M (1), N (2), and O (3), along with 10 known ones, osmanthuside B (4), osmanthuside B6 (5), acteoside (6), ligupurpuroside A (7), ligupurpuroside B (8), ligurobustoside C (9), ligurobustoside E (10), ligurobustoside I (11), cosmosiin (12), and rhoifolin (13). The structures of the new compounds were elucidated by spectroscopic methods. Seven of the glycosides showed stronger antioxidant effects than the standard, trolox.
Journal of Natural Products 07/2003; 66(6):851-4. · 3.13 Impact Factor
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ABSTRACT: Further investigation of the active components of the chloroform fraction of the seeds of Caesalpinia minax led to the isolation of a new cassane furanoditerpenoid, caesalmin H (1), together with two known furanoditerpenoid lactones, caesalmin B (2) and bonducellpin D (3). Reduction of the naturally abundant caesalmin D (9), E (10) and F (11) resulted in three new furanoditerpenoid derivatives 4-6. Phytochemical study of the stem of the same plant and subsequent reduction afforded two friedelane triterpenoids (7-8), which were identified by spectroscopic methods. Compounds 1-2 and 4-8 were corroborated by single crystal X-ray analysis. The factors governing the reduction of cassane furanoditerpenoids and friedelane triterpenoids were investigated by correlating the crystallographic results with density functional theory. The inhibitory activities of 2-8 on the Para3 virus were evaluated by cytopathogenic effects (CPE) reduction assay.
Bioorganic & Medicinal Chemistry 08/2002; 10(7):2161-70. · 2.92 Impact Factor
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ABSTRACT: The flower of Trollius chinensis Bunge is used for treating upper respiratory infections, pharyngitis, tonsillitis, and bronchitis in Chinese folk medicine. The antiviral activities of the crude extract, total flavonoids, orientin, vitexin and proglobeflowery acid isolated from the flowers of T. chinensis against parainfluenza type 3 (Para 3) virus were investigated. The results showed that the crude extract and total flavonoids exhibited a weak antiviral activity against Para 3. Orientin and vitexin demonstrated potent or moderate antiviral activity against Para 3. Proglobeflowery acid showed weak antiviral activity against Para 3.
Journal of Ethnopharmacology 04/2002; 79(3):365-8. · 3.01 Impact Factor
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ABSTRACT: Forty-four medicinal herbs were tested for antiviral activities against respiratory syncytial virus (RSV) by means of the cytopathologic effect (CPE) assay. Twenty-seven of the 44 medicinal herbs showed potent or moderate antiviral activities against RSV with 50% inhibition concentration (IC(50)) ranging from 6.3 to 52.1 microg/ml, and with selectivity index (SI) ranging from 2.0 to 32.1. Further purification of the active extracts from Sophora flavescens Ait. and Scutellaria baicalensis Georgi led to the identification of anagyrine (2), oxymatrine (7), sophoranol (10), wogonin (12), and oroxylin A (13) as the potent anti-RSV components.
Journal of Ethnopharmacology 03/2002; 79(2):205-11. · 3.01 Impact Factor
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Tetrahedron Letters 43(13):2415-2418. · 2.68 Impact Factor
