Publications (8)9.42 Total impact
-
Article: Murine RANK protein's inhibition of bone resorption.
[show abstract] [hide abstract]
ABSTRACT: The objective was to study the inhibitory effects of recombinant murine receptor activator of nuclear factor κB (RANK) protein on osteoclasts in vivo and in vitro. The RANK protein was added to the cocultures of osteoclasts at concentrations of 10(-6), 10(-5), and 10(-4) g/L. The morphology and number of osteoclasts were examined. Female KM mice were ovariectomized and treated with RANK protein at 5 mg/kg body weight. Biochemical markers of bone metabolism, bone mineral density, and bone morphology were examined. Three days after RANK treatment, the numbers of tartrate-resistant acid phosphatase-positive osteoclasts and resorption pits in bone slices decreased significantly in each treatment group, with the most significant decrease observed in the 10(-4) g/L group. Compared with the control group in vivo, the RANK-treated group exhibited higher bone mineral density and nearly complete inhibition of tartrate-resistant acid phosphatase-positive osteoclasts in bone slices. Recombinant murine RANK protein effectively inhibits the activity of osteoclasts and the resulting bone resorption.The Journal of craniofacial surgery 11/2011; 22(6):2084-9. · 0.81 Impact Factor -
Article: [Effectiveness of AO anatomical locking compression plate in treating type C distal humeral fracture].
[show abstract] [hide abstract]
ABSTRACT: To evaluate the effectiveness of the AO anatomical locking compression plate in treating type C distal humeral fracture. Between July 2008 and April 2009, 13 cases of type C distal humeral fracture were treated with the AO anatomical locking compression plates. There were 5 males and 8 females with an average age of 52.1 years (range, 24-80 years). Fractures were caused by tumbling in 7 cases, by traffic accident in 4 cases, and by falling from height in 2 cases. According to Association for Osteosynthesis/Orthopaedic Trauma Association (AO/OTA) classification, there were 3 cases of type C1, 6 cases of type C2, and 4 cases of type C3. Two cases complicated by ulnar nerve injuries, 1 by radial nerve injury, 2 by fractures of ulnar olecranon, 3 by fractures of other parts of extremities, and 6 by osteoporosis. The time from injury to hospitalization ranged from 3 hours to 4 days (0.9 day on average). All the incisions achieved healing by first intention. Thirteen cases were followed up 12 to 21 months with an average of 15.9 months. According to the X-ray films, unions were achieved both at fracture site and the olecranon osteotomy site with a healing time of 8 to 13 weeks (10 weeks on average). The function of elbows recovered from 3 to 32 weeks (10 weeks on average). No fixation failure, myositis ossificans, delayed union, or malunion occurred during the follow-up. The Mayo Elbow Performance score ranged from 75 to 100 with an average score of 95.8; the results were excellent in 9 cases, good in 3 cases, and fair in 1 case with an excellent and good rate of 92.3%. CONCLUSION : The AO anatomical locking compression plate has a good fixation in treating type C distal humeral fracture. Through the approach of olecranon osteotomy, it is easy to get anatomical reduction, stable fixation, and early exercise.Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery 12/2010; 24(12):1409-12. -
Article: [Comparison of effectiveness of three operations in treatment of displaced femoral neck fractures in the elderly patients].
[show abstract] [hide abstract]
ABSTRACT: To compare the effectiveness of internal fixation, hemiarthroplasty, and total hip arthroplasty in the treatment of displaced femoral neck fractures in elderly patients so as to provide the evidence for the selection of therapeutic methods. Between May 2005 and April 2008, 108 elderly patients with displaced femoral neck fractures were treated by internal fixation with compression screw (IF group, n = 31), hemiarthroplasty (HA group, n = 37), and total hip arthroplasty (THA group, n = 40). In IF group, there were 8 males and 23 females with an average age of 73 years (range, 65-80 years); fractures were caused by tumbling (25 cases) and traffic accident (6 cases), including 17 cases of Garden type III and 14 cases of Garden type IV; and the time from injury to operation ranged from 8 hours to 13 days with an average of 4.2 days. In HA group, there were 10 males and 27 females with an average age of 74 years (range, 65-80 years); fractures were caused by tumbling (29 cases) and traffic accident (8 cases), including 21 cases of Garden type III and 16 cases of Garden type IV; and the time from injury to operation ranged from 1 to 14 days with an average of 4.4 days. In THA group, there were 11 males and 29 females with an average age of 73 years (range, 66-80 years); fractures were caused by tumbling (32 cases) and traffic accident (8 cases), including 23 cases of Garden type III and 17 cases of Garden type IV; and the time from injury to operation ranged from 2 to 14 days with an average of 5.6 days. There was no significant difference in general data among 3 groups (P > 0.05). There were significant differences in operation time and blood loss among 3 groups (P < 0.05), and IF group was less than other 2 groups. All patients were followed up 1 year and 4 months to 2 years and 3 months with an average of 1 year and 8 months. In IF group, HA group, and THA group, the rates of early postoperative complications were 19.4% (6/31), 8.1% (3/37), and 7.5% (3/40), respectively; the rates of late postoperative complications were 29.0% (9/31), 13.5% (5/37), and 7.5% (3/40), respectively; and the reoperation rates were 29.0% (9/31), 10.8% (4/37), and 5.0% (2/40), respectively. The rates of the early postoperative complication, late postoperative complication, and reoperation rate were significantly higher in IF group than in HA group and THA group (P < 0.05), but there was no significant difference between HA group and THA group (P > 0.05). The mortality rates were 16.1% (5/31), 13.5% (5/37), and 15.0% (6/40) in IF group, HA group, and THA group, respectively; showing no significant difference (P > 0.05). According to Harris hip score, the excellent and good rates were 65.4% (17/26), 81.3% (26/32), and 85.3% (29/34) in IF group, HA group, and THA group, respectively; showing significant differences among 3 groups (P < 0.05). According to patient's age, life expectancy, and general conditions, THA is a reasonable choice for the patients aged 65-80 years with displaced femoral neck fracture.Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery 12/2010; 24(12):1419-23. -
Article: [Effectiveness of acetabular transverse and posterior wall fractures by Kocher-Langenbeck approach].
[show abstract] [hide abstract]
ABSTRACT: To investigate the surgical treatment effectiveness of acetabular transverse and posterior wall fractures by Kocher-Langenbeck approach. Between January 2002 and February 2009, 17 patients with acetabular transverse and posterior wall fractures were treated with Kocher-Langenbeck approach and fracture reduction and fixation. There were 12 males and 5 females with an average age of 33.4 years (range, 20-65 years). The disease causes were traffic accident in 16 cases and falling from height in 1 case. The disease duration was 6 hours to 11 days. According to Letournel classification, all fractures were rated as acetabular transverse and posterior wall fractures. Concomitant injuries included posterior hip dislocation in 3 cases, fracture of extremities in 8 cases, injury of sciatic nerve in 3 cases, craniocerebral injury in 1 case, and lienal rupture in 1 case. The incisions healed primarily and no complication of infection and deep venous thrombosis occurred after operation. All patients were followed up 12 to 36 months with an average of 19 months. The X-ray films showed that fracture healed 3 to 5 months after operation. After operation, anatomic reduction was found in 9 cases, satisfactory reduction in 5 cases, and unsatisfactory reduction in 3 cases according to Matta et al criterion. According to modified grading system of Merle D'Aubigne and Postel, the results were excellent in 4 cases, good in 9, fair in 3, and poor in 1 at last follow-up with an excellent and good rate of 76.5%. The nerve function was recovered in patients with sciatic nerve injury at 12 months after symptomatic treatment. Traumatic arthritis occurred in 5 cases, avascular necrosis of the femoral head in 1, and heterotopic ossification in 5 between 9 weeks and 12 months after operation. For acetabular transverse and posterior wall fractures, it is important to make adequate preoperative preparation, to get the imaging data, and to perform open reduction and internal fixation with Kocher-Langenbeck approach as early as possible.Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery 12/2010; 24(12):1428-31. -
Article: Ultrasound biomicroscopy for the detection of early osteoarthritis in an animal model.
[show abstract] [hide abstract]
ABSTRACT: Osteoarthritis (OA) is a common disease, and early diagnosis is essential for preventing further cartilage destruction and decreasing severe complications. Ultrasound biomicroscopy (UBM) is sensitive for detecting minute lesions in tissue because of its higher resolution, but its B-mode characterization of the early stage of OA has not been widely studied. The aim of this study was to determine the usefulness of UBM for detecting the early stage of OA using a rabbit model of early OA. Eighteen adult New Zealand White female rabbits were used in this study, which included 12 rabbits that underwent transections of the left anterior cruciate ligament and six control rabbits. At 2, 4, and 6 weeks after surgery, four experimental rabbits and two control rabbits were euthanized. UBM was performed to evaluate the articular cartilage surfaces of the left knee, using a 55-MHz transducer. All the articular cartilage surfaces were independently assessed in blinded fashion by two radiologists for the severity of OA. The value of UBM, interobserver reliability, and the concordance between UBM and pathologic grades were determined. For the first radiologist, the sensitivity, specificity, positive predictive value, and negative predictive value of UBM for the diagnosis of OA were 91%, 83%, 89%, and 86%, respectively. For the second radiologist, the sensitivity, specificity, positive predictive value, and negative predictive value of UBM were 93%, 86%, 91%, and 89%, respectively. The concordance between UBM and pathologic grades for both radiologists was high (κ = 0.72 and 0.76), and the interobserver agreement was high (κ = 0.80). UBM can be used to evaluate cartilage defects in an animal model, and further study is needed to determine whether this technique can be valuable for detecting early OA in humans.Academic radiology 11/2010; 18(2):167-73. · 2.09 Impact Factor -
Article: Inhibition of the osteoclast activity with the application of recombinant murine RANK protein.
[show abstract] [hide abstract]
ABSTRACT: To investigate the effects of recombinant Murine RANK on the osteoclast activity. Osteoclast was observed with soluble RANK. Female Wistar rats were bilaterally ovariectomized, and intra-abdominally injected with 5 mg/Kg soluble RANK. The bone metabolism, bone density, and bone histomorphology were observed. Compared with the control group, the quantity of TRAP-positive osteoclasts and bone resorption pit counting in the rest groups were significantly reduced. The bone density of the dosed groups was significantly increased and TRAP-stained osteoclasts in bone tissue sections were almost inhibited. rh-Murine RANK was able to inhibit osteoclast differentiation and prevent ovariectomy-induced bone loss.Artificial Cells Blood Substitutes and Biotechnology (formerly known as Artificial Cells Blood Substitutes and Immobilization Bi 04/2010; 38(4):169-77. · 0.94 Impact Factor -
Article: Comparisons of collagen gel and collagen matrix as cell growth microenvironment: the physical-chemical properties.
[show abstract] [hide abstract]
ABSTRACT: To evaluate the changes of gel scaffold and spongy scaffold in fibroblast culture in vitro, two kinds of collagen dermis were constructed in this research. Type I collagenase and Dispase were used to isolate dermis fibroblast from neonate prepuce. The gel dermis was constructed by mixing the fibroblast and collagen swelling solution, and the collagen spongy dermis was also constructed. After histological and immunohistochemical staining, these two dermis' properties were compared. Compared with gel dermis, the spongy dermis possessed more superior tension strength, but it decreased significantly during the culture. The transition temperature in DSC reduced also. The rupture length of gel dermis increased, on the contrary. Gel dermis contained more moisture than spongy dermis. It had been observed that the collagen gel was more suitable for fibroblast in maintaining its morphology than spongy scaffold, though with less cell number. The reason might be due to the special structure of collagen gel. Water in gel is sub-bound water, which is different from free water, and it is suitable for fibroblasts to secrete extracellular matrix. It could be concluded that the collagen gel dermis is better than collagen spongy dermis in the biological property.Artificial Cells Blood Substitutes and Biotechnology (formerly known as Artificial Cells Blood Substitutes and Immobilization Bi 07/2009; 37(4):166-72. · 0.94 Impact Factor -
Article: Effect of chitosan as a dispersant on collagen-hydroxyapatite composite matrices.
[show abstract] [hide abstract]
ABSTRACT: Hydroxyapatite (HA) powder is difficult to disperse evenly in collagen (Col) gelatum, which can affect the properties of the final Col-HA composite. Here, we used chitosan (CS) as a dispersant in a bone matrix constructed with Col and Ca(5)(PO(4))(3)OH (HA), which are the main components of natural bone. Solid-liquid phase separation was used to shape a three-dimensional porous structure to support cell growth. The two bone matrices (Col-CS-HA and Col-HA) were investigated by transmission electron microscopy, scanning electron microscopy, and electron spectroscopy for chemical analysis for structural characteristics and physicochemical properties. Their ability to repair bone defects was evaluated in vivo. HA particles were dispersed in Col gelatum evenly by mixing with CS gelatum. The surface morphology and three-dimensional structure of the matrix became more regular, which improved cell growth. After CS modification, the percentage of C-N groups increased to 6.40% and C-OH groups increased from 1.05% to 6.92%. The total amount of nonpolar groups (C-C groups and C-H groups) decreased from 82.48% to 74.05%. The vitrification and denaturation temperatures increased from 40.59 degrees C and 106.36 degrees C, respectively, to 42.23 degrees C and 111.04 degrees C, respectively. CS modified the surface chemistry to create a favorable environment for osteoblast adhesion and proliferation in vivo, which would accelerate the process of bone regeneration. The Col-CS-HA matrix possessed superior biological and mechanical properties for bone defect repair.Tissue Engineering Part C Methods 05/2009; 16(1):71-9. · 4.64 Impact Factor
