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Publications (6)1.51 Total impact

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    ABSTRACT: In recent years, emerging data support the concept of endothelial dysfunction in the course of age-related macular degeneration (AMD). Circulating endothelial cells (CECs) are desquamated mature cells that have detached from the intimal monolayer in response to endothelial injury. In this study we sought to explore the potential role of endothelial dysfunction in pathogenesis of AMD by measuring the concentration of CECs in peripheral blood of AMD patients. Peripheral blood samples from 31 patients with diagnosed dry AMD and 46 patients with neovascular AMD were collected. Forty six, age- and sex-matched volunteers without AMD were enrolled as a control group. CECs were counted and analyzed by flow cytometry. Multivariate analyses of patients and controls adjusted for age, gender, presence of ischemic heart disease, hypertension and smoking (current or past) revealed that both wet (beta = +0.45, p = 0.0003) and dry (beta = +0.28, p = 0.027) forms of AMD are independent factors associated with higher number of CECs. Our results suggest that there is an endothelial alteration accompanying AMD. Increased numbers of CECs AMD patients reflect a severe vascular disturbance and may contribute to the disease process. These findings can help expand our knowledge of the pathogenic mechanisms of AMD and may be relevant to the potential treatment of this disease.
    Klinika oczna 01/2011; 113(7-9):228-32.
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    ABSTRACT: Stem cells (SCs) maintain the balance among somatic cell populations in various tissues and are responsible for organ regeneration. The remarkable progress of regenerative medicine in the last few years indicates promise for the use of SCs in ophthalmic disorder treatment. This review describes the current view on hierarchy in the SC compartment and presents the latest attempts to use adult SCs in the regeneration of the retina. Research performed primarily in animal models gives hope for using similar strategies in humans. However, the search for the optimal source of SCs for cell therapy continues. We briefly discuss various potential sources of adult SCs that could be employed in regenerative medicine, particularly focusing on recently identified, very small embryonic-like SCs (VSEL-SCs). These cells are even present in the bone marrow and adult tissues of older patients and could be harvested from cord blood. We believe that VSEL-SCs, after the establishment of ex vivo expansion and differentiation protocols, could be harnessed for retina regeneration.
    Current eye research 09/2009; 34(9):748-60. · 1.51 Impact Factor
  • Leszek Kuprjanowicz, Danuta Karczewicz
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    ABSTRACT: The aim of this research is to describe one of the most frequent ophthalmology disease-glaucoma and to look at their neurodegenerative nature and methods to stop disease. We use neuroprotection in glaucoma from a short time but despite of ethiological reasons neuroprotection seems to be one of the main mechanism to slow their progression. This research is talking about new opinions about neuroprotection in glaucoma and their epidemiology and etiology.
    Annales Academiae Medicae Stetinensis 02/2007; 53 Suppl 1:22-9.
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    ABSTRACT: To assess the evaluation ability of retinal nerve fiber layer (RNFL) in patients with visual tracts' compression and to assess the correlation between visual field loss and changes in RNFL. Polarimetric RNFL analysis (scanning laser polarimeter- NFA/GDx, software version 1.0.16, LDT(inc)) and kinetic perimetry were done in 41 eyes with different types of optic chiasma compression and in 40 healthy eyes, age matched controls. In eyes with optic chiasma compression most of the GDx parameters were statistically different from the control group (P<0.05). In eyes with normal visual fields and with optic chiasma compression most of GDx parameters were statistically different from the other analyzed groups. GDx may be a helpful tool in diagnosis of optic chiasma diseases. GDx is a useful visual prognostic indicator in the preoperative assessment of optic chiasma compression. Objective RNFL assessment is especially important in patients with serious visual field loss and poor visual acuity.
    Klinika oczna 02/2005; 107(7-9):452-6.
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    ABSTRACT: To measure retinal nerve fiber layer thickness (RNFL) in patients with anterior ischemic optic neuropathy (AION) by scanning laser polarimetry and to asses the correlation between RNFL thickness and perimetric indices in AION eyes. Polarimetric RNFL analysis (scanning laser polarimeter- NFA/GDx, software version 1.0.16, LDTinc) and standard automated perimetry (SAP- HFA, 24-2 thresold test) was done in 28 AION eyes and in 14 healthy age matched controls. In AION eyes most of the GDx parameters were statistically different from the control group (P<0.05). Some of the GDx parameters strongly correlated with SAP indices (MD) in AION eyes. RNFL thickness measured with NFA/GDx is thinner in AION eyes than in healthy eyes and correlates with visual field loss.
    Klinika oczna 01/2004; 106(3 Suppl):440-2.
  • Danuta Karczewicz, Monika Modrzejewska, Leszek Kuprjanowicz
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    ABSTRACT: The aim of this research was to evaluate the changes of blood flow velocity in ocular arteries (ophthalmic artery, central retinal artery and short posterior ciliary arteries) and the amount of ocular nerve fibers in patients with primary open angle glaucoma (POAG) and normal tension glaucoma (NTG). The research was done on 17 patients with diagnosed POAG and 13 patients with NTG. No ocular surgery was performed on any of these patients prior to our examinations. The blood flow measurements were made with ACUSON Color Doppler USG 128*P10 type with 7.5 MHz sector phased probe. Parameters of diastolic velocity (Vr), systolic flow velocity (Vs), Golsing pulsation index (PI) and pourcelate resistivity index (RI) were registered, together with the instant spectrography of examined artery. Quantitative retinal nerve fiber layer (RNFL) measurements were obtained by scanning laser polarimetry GDx (software version 1.0.14). RNFL was measured automatically in each of 256x 256 pixels and results were compared to the age-, sex- and race-norms. Approximately 150 wide field of view was used. Average thickness, Superior Maximum, Inferior Maximum, maximum modulation and GDx Number were analysed. All results were compared to age norms. Student's t test was used for statistical analysis. In patients with POAG a statistically significant decrease of Vs in ophthalmic artery was detected in 27% of eyes, in central retinal artery in 51% of eyes and in short posterior ciliary arteries in 45% of eyes. In patients with NTG a statistically significant decrease of Vs was detected in ophthalmic artery in 27% of eyes, in central retinal artery in 65% of eyes and in short posterior ciliary arteries in 53% of eyes. RNFL analysis in eyes with POAG showed a decrease in avg. thickness in 80% of analysed eyes, decrease of Superior Maximum in 84% of analysed eyes, decrease of Inferior Maximum in 77.4% of analysed eyes, decrease in maximal modulation in 87% of analysed eyes and increase of GDx Number in 77% of analysed eyes. RNFL analysis in patients with NTG showed a decrease in av. thickness in 73% of analysed eyes, decrease of Superior Maximum in 80.7% of analysed eyes, decrease of Inferior Maximum in 65.7% of analysed eyes, decrease in maximal modulation in 61.5% of analysed eyes and increase of GDx Number in 57.6% of analysed eyes.
    Klinika oczna 02/2002; 104(3-4):207-10.