Hai-Tao Xiao

Hong Kong Baptist University, Kowloon, Hong Kong

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Publications (14)23.6 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: The therapeutic effect of corilagin (1) was evaluated in an acute colitis model induced by dextran sulfate sodium (DSS) in mice, and the mechanism of action was investigated in this study. Animals were challenged with 2% DSS drinking water for 5 consecutive days and then intraperitoneally treated with 1 (7.5, 15, and 30 mg/kg) daily for 7 days. It was found that 1 significantly decreased the disease activity index, inhibited the shortening of colon length, reduced colon tissue damage, and suppressed myeloperoxidase activity. Moreover, 1 greatly suppressed the secretion of TNF-α, IL-6, and IL-1β, inhibited the degradation of IκB α, and down-regulated expression of cleaved caspase-3 and cleaved caspase-9 in colon tissues of DSS-treated mice. These findings demonstrated that 1 exerts a protective effect on DSS-induced colitis, and its underlying mechanisms are associated with inhibition of the NF-κB pathway that mitigates colon inflammatory responses and apoptosis of intestinal epithelial cells.
    Journal of Natural Products 11/2013; · 3.29 Impact Factor
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    ABSTRACT: Abstract Context: Polygonum cuspidatum Sieb et Zucc. (Polygonaceae) possesses various pharmacological activities and has been widely using as one of the most popular and valuable Chinese herbal medicines in clinics. Its usage has increasingly attracted much of our attention and urges investigation on its bioactive components. Objective: To establish a rapid and valid approach for screening potential neuroprotective components from P. cuspidatum. Materials and methods: Potential neuroprotective components from P. cuspidatum were screened utilizing liposome equilibrium dialysis followed by high-performance liquid chromatography (HPLC) analysis. Their neuroprotective effects on modulation of protein expression of α7 nAChR, α3 nAChR and synaptophysin (SPY) on SH-SY5Y human neuroblastoma cell line (SH-SY5Y) were evaluated by means of Western blotting. Results: Two potential compounds, polydatin (C1) and emodin-8-O-β-d-glucoside (C2), were detected and identified in our study. The biological tests showed that both compounds C1 and C2, respectively, at concentrations of 0.1 and 0.25 mg/mL significantly increased protein expression of α7 and α3 nicotinic acetylcholine receptors (nAChRs) in SH-SY5Y cells. Moreover, C1 and C2 at 0.1 mg/mL significantly reversed the Aβ1-42-induced decrease of α7 and α3 nAChRs protein expression in SH-SY5Y cells. In addition, C2 at 0.1 mg/mL significantly increased protein expression of SPY in SH-SY5Y cells and Aβ1-42-induced SH-SY5Y cells whereas C1 did not provide any positive effects. Discussion and conclusion: In conclusion, our approach utilizing liposome equilibrium dialysis combined with HPLC analysis and cell-based assays is a prompt and useful method for screening neuroprotective agents.
    Pharmaceutical Biology 10/2013; · 1.21 Impact Factor
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    ABSTRACT: Polygonum chinense Linn., a folk medicine, has long been used for the treatment of diarrhea and enteritis in southwestern China. However, the components responsible for its anti-diarrheal activity are still poorly understood. To determine anti-diarrheal activities of P. chinense L. and to identify its active components through bioactivity-guided isolation technique. Animals were orally administered with the extract of P. chinense L.. The anti-diarrheal effects of 75% ethanol extract, four fractions with different polarities from 75% ethanol extract, different eluates collected from Diaion HP-20 macroporous resin chromatography, ellagic acid and corilagin, were examined based on mouse models of castor oil- and magnesium sulfate-induced diarrhea. The results showed that the 75% ethanol extract of P. chinense L. exhibited significant anti-diarrheal activities in a dose-dependent manner in two mouse models. Through in vivo bioactivity-guided fractionation processes, n-butanol and aqueous fractions were found to exhibit prominent anti-diarrheal activities, and two major compounds, ellagic acid and corilagin, from these active fractions were found to possess anti-diarrheal effects. Present study provides evidence of the utilization of P. chinense L. for diarrhea, and ellagic acid and corilagin are two components contributing to the anti-diarrheal effect.
    Journal of ethnopharmacology 07/2013; · 2.32 Impact Factor
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    ABSTRACT: To investigate the key factors in developing the trinitrobenzene sulfonic acid (TNBS)-induced post-inflammatory irritable bowel syndrome (PI-IBS) model in rats. TNBS was administered to rats at the following conditions: (1) with different doses (20, 10, 5 mg/0.8 mL per rat); (2) with same dose in different concentrations (20 mg/rat, 25, 50 mg/mL); (3) in different ethanol percentage (25%, 50%); and (4) at depth either 4 cm or 8 cm from anus. At 5 d and 4 wk after TNBS administration, inflammation severity and inflammation resolution were evaluated. At 4 and 8 wk after TNBS application, visceral hyperalgesia and enterochromaffin (EC) cell hyperplasia were assayed by abdominal withdrawal reflex test, silver staining and capillary electrophoresis. Our results showed that: (1) TNBS induced dose-dependent acute inflammation and inflammation resolution. At 5 d post TNBS, the pathological score and myeloperoxidase (MPO) activity in all TNBS treated rats were significantly elevated compared to that of the control (9.48 ± 1.86, 8.18 ± 0.67, 5.78 ± 0.77 vs 0, and 3.55 ± 1.11, 1.80 ± 0.82, 0.97 ± 0.08 unit/mg vs 0.14 ± 0.01 unit/mg, P < 0.05). At 4 wk post TNBS, the pathological score in high and median dose TNBS-treated rats were still significantly higher than that of the control (1.52 ± 0.38 and 0.80 ± 0.35 vs 0, P < 0.05); (2) Intracolonic TNBS administration position affected the persistence of visceral hyperalgesia. At 4 wk post TNBS, abdominal withdrawal reflex (AWR) threshold pressure in all TNBS-treated groups were decreased compared to that of the control (21.52 ± 1.73 and 27.10 ± 1.94 mmHg vs 34.44 ± 1.89 mmHg, P < 0.05). At 8 wk post TNBS, AWR threshold pressure in 8 cm administration group was still significantly decreased (23.33 ± 1.33 mmHg vs 36.79 ± 2.29 mmHg, P < 0.05); (3) Ethanol percentage affected the TNBS-induced inflammation severity and visceral hyperalgesia. In TNBS-25% ethanol-treated group, the pathological score and MPO activity were significantly lowered compared to that of the TNBS-50% ethanol-treated group, while AWR threshold pressure were significantly elevated (36.33 ± 0.61 mmHg vs 23.33 ± 1.33 mmHg, P < 0.05); and (4) TNBS (5 mg/0.8 mL per rat, in 50% ethanol, 8 cm from anus)-treated rats recovered completely from the inflammation with acquired visceral hyperalgesia and EC cell hyperplasia at 4 wk after TNBS administration. TNBS dosage, concentration, intracolonic administration position, and ethanol percentage play important roles in developing visceral hyperalgesia and EC cell hyperplasia of TNBS-induced PI-IBS rats.
    World Journal of Gastroenterology 05/2012; 18(20):2481-92. · 2.55 Impact Factor
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    ABSTRACT: The present study aimed to investigate the analgesic effect of JCM-16021, a revised traditional Chinese herbal formula, on postinflammatory irritable bowel syndrome (PI-IBS) in rats. The trinitrobenzene sulfonic (TNBS) acid-induced PI-IBS model rats were orally administrated with different doses of JCM-16021 (1.2, 2.4, and 4.8 g/kg/d) for 14 consecutive days. The results showed that JCM-16021 treatment dose-dependently attenuated visceral hyperalgesia in PI-IBS rats. Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated. Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment. These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.
    Evidence-based Complementary and Alternative Medicine 01/2012; 2012:239638. · 1.72 Impact Factor
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    ABSTRACT: In a bioassay-guided search for acetylcholinesterase (AChE) inhibitors from Chinese natural resources, eight isoquinoline alkaloids, tetrahydropalmatine (1), corydaline (2), protopine (3), berberine (4), palmatine (5), jatrorrhizine (6), coptisine (7) and dehydrocorydaline (8), were isolated from the methanolic extract of the tubers of Corydalis yanhusuo. Structures of these compounds were identified by spectroscopic techniques. Compounds 4-8 inhibited AChE activity in a dose-dependent manner, and the IC₅₀ values were 0.47 ± 0.01, 0.74 ± 0.06, 2.08 ± 0.09, 1.01 ± 0.03 and 0.62 ± 0.05 µM, respectively. Structure-activity relationship analysis suggested that aromatisation at ring C, as well as substitutions at C-2, C-3, C-9, C-10 and C-13 affect the AChE activity of protoberberine alkaloids.
    Natural product research 03/2010; 25(15):1418-22. · 1.01 Impact Factor
  • Chemistry of Natural Compounds 07/2009; 45(4):539-541. · 0.60 Impact Factor
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    ABSTRACT: Two new norbisabolane sesquiterpenoid glycosides, glochicoccinosides A (1) and B (2), together with two known compounds, have been isolated from the rhizomes of Glochidion coccineum. Their structures were elucidated by the combination of 1D NMR, 2D NMR, and MS spectral analysis, as well as chemical evidence. Cytotoxic activities and the antioxidant effect of these compounds were evaluated, but none of them showed activity.
    Journal of Asian Natural Products Research 01/2008; 10(1-2):1-5. · 0.95 Impact Factor
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    ABSTRACT: A novel lignanoid, named glochinin A (1), along with two known norbisabolane sesquiterpenoids, namely glochicoccin D (2) and phyllaemblic acid (3), were isolated from Glochidion puberum. Their structures were elucidated by spectral analysis.
    Chemistry of Natural Compounds 01/2008; 44(5):588-590. · 0.60 Impact Factor
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    ABSTRACT: Two new phenols, 6'- O-vanilloylisotachioside ( 1) and 6'- O-vanilloyltachioside ( 2), together with nine known compounds, were isolated from the leaves of Baccaurea ramiflora (Euphorbiaceae). The structures of the new compounds were elucidated mainly by analysis of physical and spectroscopic data. Compounds 1 - 10 were tested for antioxidant activities by using MTT and DPPH assays. Seven compounds, 1, 2, and 4 - 8, revealed potent antioxidant activities against H (2)O (2)-induced impairment in PC12 cells, and exhibited significant DPPH radical-scavenging activities with IC (50) values of 86.9, 142.9, 15.2, 37.6, 35.9, 30.2, and 79.8 microM, respectively.
    Planta Medica 11/2007; 73(13):1415-7. · 2.35 Impact Factor
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    ABSTRACT: Two novel chalcone constituents, tarennane (1) and tarennone (2), together with nine known compounds, were isolated from the whole plant of Tarenna attenuata. By analysis of physical and spectroscopic data, the structures of new compounds were elucidated as (E)-4-[3-(4-hydroxy-3-methoxyphenyl)-acryl]-3,4,5-trimethoxycyclohexa-2,5-dienone (1) and 1,2-bis(3,5-dimethoxy-4-hydroxyphenyl)-3-hydroxypropan-1-one (2). These two compounds were tested for antioxidant activities in the MTT and DPPH assays. Compound 1 revealed potent antioxidant activities against H2O2-induced impairment in PC12 cells, but neither of them showed DPPH radical-scavenging activity with IC50 values of 181.1 and 210.3 microM, respectively.
    Planta Medica 06/2007; 73(5):496-8. · 2.35 Impact Factor
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    ABSTRACT: Six new mixed lignan-neolignans and 20 known compounds were isolated from the whole plant of Tarenna attenuata. By analysis of physical and spectroscopic data, the structures of the new compounds were elucidated as (1R,5R,6R)-6-{4-O-[2-(1-(4-hydroxy-3-methoxyphenyl))glycerol]-3,5-dimethoxyphenyl}-3,7-dioxabicyclo[3.3.0]octan-2-one (1), 5' '-methoxyhedyotisol A (2), 4' '-O-(8-guaiacylglycerol)buddlenol A (3), 5' '-methoxy-4' '-O-(8-guaiacylglycerol)buddlenol A (4), 4,6-dimethoxy-5-hydroxy-3-hydroxymethyl-2-(3,4,5-trimethoxyphenyl)-2,3-dihydrobenzofuran (5), and 7-O-ethylguaiacylglycerol (6). Compounds 1, 5, 6, and 8 showed potent antioxidant activities against H2O2-induced impairment in PC12 cells, and compounds 1, 2, 5, and 7 scavenged DPPH radical strongly with IC50 values of 72, 87, 45, and 55 microM, respectively.
    Journal of Natural Products 05/2007; 70(4):521-5. · 3.29 Impact Factor
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    ABSTRACT: ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
    ChemInform 01/2007; 38(22).
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    ABSTRACT: A novel bisabolane-type sesquiterpenoid lactone, glochicoccin A (1), and three new norbisabolane sesquiterpenoids, glochicoccins B–D (2–4), together with two known norbisabolane sesquiterpenoids, phyllaemblic acid (5) and phyllaemblic acid methyl ester (6), were isolated from the rhizomes of Glochidion coccineum. Their structures were elucidated by different spectroscopic (IR, UV, NMR) and mass-spectrometric (MS) techniques. The structure and relative configuration of 1 was confirmed by single-crystal X-ray diffraction (Fig. 2). None of the compounds were found to exhibit cytotoxic or antioxidant activities.
    Helvetica Chimica Acta 01/2007; 90(1):164-170. · 1.38 Impact Factor

Publication Stats

45 Citations
23.60 Total Impact Points

Institutions

  • 2012–2013
    • Hong Kong Baptist University
      • School of Chinese Medicine
      Kowloon, Hong Kong
  • 2008–2010
    • Guiyang Medical University
      Kuei-yang, Guizhou Sheng, China
  • 2007
    • Kunming University of Science and Technology
      Yün-nan, Yunnan, China