Ki Hong Kim

Catholic University of Daegu, Kayō, North Gyeongsang, South Korea

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Publications (120)179.38 Total impact

  • Yue Jai Kang, Ki Hong Kim
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    ABSTRACT: Immunotherapy along with chemotherapy can be a way to enhance therapeutic efficacy and to reduce amount of chemotherapeutics. So, in the present study, we adopted an immunochemotherapy (CpG-ODN 1668 plus doxycycline) to reduce doxycycline dose to be used while at the same time to get the same treatment efficacy against scuticociliatosis in olive flounder (Paralichthys olivaceus). The survival rates of fish administered with doxycycline 100 mg/kg plus 10 μg of CpG-ODN 1668 were higher than those of fish administered with doxycycline alone at a dose of 200 mg/kg of fish weight, suggesting that coadministration of CpG-ODN 1668 can reduce doxycycline to half the dose. Furthermore, reduction of CpG-ODN 1668 to 5 μg/fish plus doxycycline 100 mg/kg led to high survival rates that were comparable to doxycycline 200 mg/kg, suggesting that the used amount of both immunotherapeutics and chemotherapeutics can be reduced through coadministration. There have been no reports on the treatment of scuticociliatosis by combining chemotherapy and immunotherapy. In the present study, we demonstrated that a combination of chemotherapy (doxycycline) and immunotherapy (CpG-ODN 1668) was effective against scuticociliatosis in olive flounder fingerlings.
    Aquaculture. 01/2015; 435:143–145.
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    ABSTRACT: Previously generated two auxotrophic genes knockout Edwardsiella tarda (Δalr Δasd E. tarda) was used as a delivery vehicle for CpG 1668 motif-enriched plasmids (pL-CpG30), and potential of the Δalr Δasd E. tarda-mediated delivery of CpG motifs in both prevention and therapeutic treatment of scuticociliatosis caused by Miamiensis avidus in olive flounder (Paralichthys olivaceus) was investigated. The auxotrophic E. tarda mutant with pL-CpG30 plasmids elicited evidently higher survival rates and delayed both onset of mortality and time-to-death of olive flounder against M. avidus challenge. Furthermore, administration of E. tarda mutant that contains plasmids enriched in CpG 1668 motif elicited clearly higher survival rates of fish that were already infected with M. avidus. These results indicate that CpG 1668 plasmids-harboring E. tarda mutant may provide not only preventive measure but also therapeutic approach against scuticociliatosis in olive flounder.
    Experimental Parasitology 06/2014; · 2.15 Impact Factor
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    ABSTRACT: Hydranencephaly is a rare congenital disease defined as an absence of cerebral hemispheres with an intact thalamus, brain stem, and cerebellum. Generally, patients with hydranencephaly require cerebro-spinal fluid diversion due to progressive hydrocephalus. An excellent view of the choroid plexus and advancement of the neurosurgical endoscope were possible, leading to easy coagulation of the choroid plexus to balance cerebro-spinal fluid production without the use of a shunt device. We present this rare case and good treatment outcome from endoscopic coagulation of the choroid plexus.
    Journal of Korean Neurosurgical Society. 06/2014; 55(6):375-8.
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    ABSTRACT: Luteolin, a flavonoid extracted from a number of plants with recognized anticancer, anti-inflammatory and anti-oxidative activities, inhibits angiogenic processes and modulates multidrug resistance. However, the efficacy and mechanisms of action of this flavonoid agent are still undergoing study. In order to elucidate whether luteolin exhibits an anticancer effect in cervical cancer cells, HeLa cells were incubated with luteolin and apoptosis was assessed by observing nuclear morphological changes, and performing Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining. Cell cycle analysis, western blotting, RT-PCR and mitochondrial membrane potential measurements were also carried out. Luteolin showed a significant dose-dependent cytotoxic effect only in human papillomavirus (HPV)-positive cervical cancer cells, when compared to its effect on HPV-negative cervical cancer C33A cells. Expression levels of human papilloma virus E6 and E7 oncogenes were suppressed, those of related factors pRb and p53 were recovered and E2F5 was increased by luteolin treatment. Furthermore, luteolin enhanced the expression of death receptors and death receptor downstream factors such as Fas/FasL, DR5/TRAIL and FADD in HeLa cells, and activated caspase cascades. In particular, luteolin enhanced the activity of caspase-3 and -8 in a dose-dependent manner. Activation of caspase-3 induced caspase-8 activity and vice versa. Luteolin also induced mitochondrial membrane potential collapse and cytochrome c release, and inhibited Bcl-2 and Bcl-xL expression. In conclusion, luteolin exerts anticarcinogenic activity through inhibition of E6 and E7 expression and cross-activation of caspase-3 and -8. Taken together, these results suggest that luteolin induces inactivation of HPV-18 oncogene expression and apoptosis by activating the intrinsic and extrinsic pathways.
    Oncology Reports 04/2014; · 2.30 Impact Factor
  • Yue Jai Kang, Dong Soo Kim, Ki Hong Kim
    Aquaculture Research 04/2014; 45(5). · 1.42 Impact Factor
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    ABSTRACT: Protection of olive flounder (Paralichthys olivaceus) from virulent Edwardsiella tarda infection by oral immunization with two auxotrophic genes – alanine racemase gene (alr) and aspartate semialdehyde dehydrogenase (asd) gene – knock-out E. tarda mutant (ΔalrΔasd E. tarda) was previously demonstrated. In the present study, we investigated the effects of cimetidine, a histamine2 (H2) receptor antagonist, on the arrival of the live auxotrophic E. tarda mutant to the intestine of olive flounder and on the protective efficacy of the live ΔalrΔasd E. tarda oral vaccine. In the experiment to determine optimal administration time of cimetidine, fish administered cimetidine at 3 h before the administration of the E. tarda mutant showed the highest bacterial colony number from the intestine. In the experiment to determine optimal dose of cimetidine, the groups of fish administered 100 mg or 200 mg of cimetidine per kg of fish showed significantly higher CFU numbers in the intestine compared to the group intubated with cimetidine 10 mg or 50 mg per kg of fish. However, as there was no significant difference between cimetidine 100 mg and 200 mg groups, a dose of 100 mg per kg of fish was chosen as the optimal dose of cimetidine. In the oral vaccine efficacy experiment, the group of fish administered the live auxotrophic E. tarda mutant in combination with the above optimal regime of cimetidine showed the highest serum agglutination activity and survival rates, suggesting that cimetidine can be used to enhance the protective efficacy of the oral live bacterial vaccines in fish. To our knowledge, this is the first study that demonstrated cimetidine-mediated enhancement of protective efficacy in oral vaccine of fish.
    Aquaculture. 01/2014; s 420–421:206–210.
  • Yue Jai Kang, Ki Hong Kim
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    ABSTRACT: Scuticociliatosis caused by Miamiensis avidus is one of the major diseases in cultured olive flounder (Paralichthys olivaceus) in Korea. However, until recently, no effective chemotherapy or vaccine has been developed. Oligodeoxynucleotides containing unmethylated deoxycytidyl-deoxyguanosine motifs (CpG-ODNs) have been shown to be effective in enhancing innate immune responses and preventing infectious diseases in fish. However, it has not been experimented whether CpG-ODNs can be used as a therapeutic tool against infectious diseases in fish. In the present study, we evaluated the therapeutic potential of CpG-ODN 1668 against scuticociliatosis in olive flounder for the first time. The group of fish administered CpG-ODN 1668 at 3 days after the infection showed clearly higher survival rates than the groups of fish administered GpC-ODN 1720 or PBS, indicating a high therapeutic potential of CpG-ODN 1668 against scuticociliatosis. However, the treatment efficacy of CpG-ODN 1668 was diminished when administered at 6 days post-infection, and no treatment effect was found when administered at 9 days after the infection. These results suggest that early diagnosis through continuous monitoring and early treatment with CpG-ODN 1668 are essential for increase of treatment efficacy. In conclusion, the present study demonstrated for the first time the possible use of CpG-ODN 1668 in treatment of scuticociliatosis in olive flounder.
    Aquaculture. 01/2014; 430:17–20.
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    ABSTRACT: The resistance against white spot syndrome virus (WSSV) infection in wild marine crab Gaetice depressus by the immunization of a recombinant glutathione-S-transferase (GST) fused VP28 protein (GST-VP28) was evaluated. The cumulative mortalities of GST-VP28 injected groups were lower than those of the control groups at 10 days of post-challenge, and the time to death of 50% crab () was delayed by the immunization using GST-VP28. The group boosted with GST-VP28 after 2 weeks of primary immunization clearly showed longer than non-boosted group against challenge with WSSV. This result suggests that boosting with the antigen protein elicit stronger immune responses similar to adaptive immune responses of vertebrates. However, the short was observed in the group challenged at 3 weeks post boosting comparing to the group challenged at 1 week post boosting. This suggests that the protective strength of immunization decreased by the time.
    Journal of fish pathology. 01/2014; 27(1).
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    ABSTRACT: The differential diagnosis of bluish-gray pigmentation of the skin includes dermal melanocytosis, malignant melanoma and metallic discoloration. Of these disorders, metallic discoloration, which develops from deposits of fine metallic particles on the skin, is an uncommon dermatologic disease. We report a case of metallic discoloration of the right shin caused by titanium alloy prostheses in a patient with right total knee replacement. The patient had bluish-gray pigmentation on her right shin as well as pain while walking. Histopathologic findings of the skin lesion showed abundant black particles in the perivascular area of the lower dermis. Polarizing microscopy revealed refractile foreign materials in the dermis. An energy dispersive X-ray spectrophotometer unit attached to a transmission electron microscope detected titanium and aluminium peaks in the specimen.
    Annals of Dermatology 08/2013; 25(3):356-9. · 0.61 Impact Factor
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    ABSTRACT: It is unclear which plaque component is related with long-term clinical outcomes in patients with coronary artery occlusive disease (CAOD). We assessed the relationship between plaque compositions and long-term clinical outcomes in those patients. The study subjects consisted of 339 consecutive patients (mean 61.7±12.2 years old, 239 males) who underwent coronary angiogram and a virtual histology-intravascular ultrasound examination. Major adverse cardiac and cerebrovascular events (MACCE), including all-cause death, non-fatal myocardial infarction, cerebrovascular events, and target vessel revascularization were evaluated during a mean 28-month follow-up period. Patients with high fibrofatty volume (FFV, >8.90 mm(3), n=169) had a higher incidence of MACCE (25.4% vs. 14.7%, p=0.015), male sex (75.7% vs. 65.3%, p=0.043), acute coronary syndrome (53.3% vs. 35.9%, p=0.002), multivessel disease (62.7% vs. 41.8%, p<0.001) and post-stent slow flow (10.7% vs. 2.4%, p=0.002) than those with low FFV (FFV≤8.90 mm(3), n=170). Other plaque composition factors such as fibrous area/volume, dense calcified area/volume, and necrotic core area/volume did not show any impact on MACCE. Cardiogenic shock {hazard ratio (HR)=8.44; 95% confidence interval (CI)=3.00-23.79; p<0.001} and FFV (HR=1.85; 95% CI=1.12-3.07; p=0.016) were the independent predictors of MACCE by Cox regression analysis. Thin-cap fibroatheroma, necrotic core area, and necrotic core volume were not associated with MACCE. FFV of a culprit lesion was associated with unfavorable long-term clinical outcomes in patients with CAOD.
    Korean Circulation Journal 06/2013; 43(6):377-83.
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    ABSTRACT: In mammals, hypoxia-inducible factor-1 α (HIF-1α) is known to play important roles not only in oxygen homeostasis but also in innate immune responses. In this study, to assess the functional role of HIF-α in respiratory burst activity of Crassostrea gigas hemocytes, oysters were injected with HIF-α- or green fluorescent protein (GFP)-targeted-long double-stranded RNAs (dsRNAs), and at 1, 3, and 7 days post-injection, knock-down of C. gigas HIF-α expression and production of reactive oxygen species (ROS) were analyzed. Expression of HIF-α in mantle, gill, and hemocytes of C. gigas was clearly down-regulated by injection of the HIF-α-targeted-long dsRNA, but was not inhibited by the GFP-targeted-long dsRNA, indicating that HIF-α expression was suppressed through sequence-specific and systemic RNA interference (RNAi). Respiratory burst activity of hemocytes was significantly increased by administration of GFP-targeted-long dsRNA. However, knock-down of HIF-α expression led to significant decrease of chemiluminescence (CL) response of C. gigas hemocytes at 3 and 7 d post-administration of HIF-α-targeted-long dsRNA, indicating the critical role of HIF-α in activation of respiratory burst activity of oyster hemocytes.
    Fish &amp Shellfish Immunology 05/2013; · 2.96 Impact Factor
  • Min Sun Kim, Ki Hong Kim
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    ABSTRACT: The role of viral hemorrhagic septicemia virus (VHSV) NV gene in nuclear factor-κB (NF-κB) activation was investigated. Epithelioma papulosum cyprini (EPC) cells pre-treated with tumor necrosis factor (TNF)-α showed a strong resistance against VHSV infection, but cells treated with TNF-α after VHSV infection showed no resistance, suggesting that immediate early TNF-α-mediated responses inhibit VHSV replication. Activation of NF-κB is a key step in TNF-α-mediated immunomodulatory pathways. In this study, activation of NF-κB by TNF-α exposure was inhibited in EPC cells harboring NV gene expressing vectors, indicating that the NV gene of VHSV can suppress TNF-α-mediated NF-κB activation. Furthermore, the NV gene knock-out recombinant VHSV (rVHSV-ΔNV-EGFP) induced significantly higher NF-κB activity in EPC cells than wild-type VHSV, suggesting that VHSV adopted a strategy to suppress early activation of NF-κB in host cells through and NV gene.
    Fish &amp Shellfish Immunology 03/2013; · 2.96 Impact Factor
  • Min Sun Kim, Ji Sun Park, Ki Hong Kim
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    ABSTRACT: AIM: As the strength and duration of immune responses can be regulated by the antigen dose, higher expression of foreign antigens in the viral-vectored vaccines would be an important factor for inducing effective immune responses. The aim of this study was to determine the optimal insertion place of a foreign antigen gene in the genome of viral hemorrhagic septicemia virus (VHSV) for development of VHSV-based viral-vectored vaccines. METHODS AND RESULTS: Recombinant VHSVs (rVHSVs) harboring the red fluorescent protein (RFP) gene between N and P (rVHSV-A-RFP), P and M (rVHSV-B-RFP), or M and G genes (rVHSV-C-RFP) in the genome were rescued by reverse genetics. Their replication ability and expression level of RFP were compared according to the inserted locations. The viral titers of each rVHSV were not significantly different. However, Epithelioma papulosum cyprini (EPC) cells infected with rVHSV-A-RFP or rVHSV-B-RFP showed clearly higher fluorescence than cells infected with rVHSV-C-RFP. There was no significant difference in RFP expression between cells infected with rVHSV-A-RFP and rVHSV-B-RFP. CONCLUSIONS: The present results indicate that insertion of a foreign gene between N and P, or P and M genes of VHSV genome would be advantageous for development of VHSV-based viral-vectored vaccines. SIGNIFICANCE AND IMPACT OF THE STUDY: The present work is the first report on the optimal location of a foreign gene in VHSV genome for high expression, and the locations identified in this study would be suitable for the development of VHSV-based viral-vectored vaccines. © 2013 The Authors Journal of Applied Microbiology © 2013 The Society for Applied Microbiology.
    Journal of Applied Microbiology 02/2013; · 2.20 Impact Factor
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    ABSTRACT: BACKGROUND: Parasite peptidases have been actively studied as vaccine candidates or drug targets for prevention or treatment of parasitic diseases because of their important roles for survival and/or invasion in the host. Like other parasites, the facultative histophagous ciliate Miamiensis avidus would possess peptidases that are closely associated with the invasion into the host tissue and survival in the host. RESULTS: The 17 genes encoding peptidases, including seven cathepsin-like cysteine peptidases, four serine carboxypeptidases, a eukaryotic aspartyl protease family protein, an ATP-dependent metalloprotease FtsH family protein, three leishmanolysin family proteins and a peptidase family M49 protein were identified from a Miamiensis avidus cDNA library by BLAST X search. Expression of genes encoding two cysteine peptidases, three leishmanolysin-like peptidases and a peptidase family M49 protein was up-regulated in the cell-fed ciliates compared to the starved ciliates. Especially, one cysteine peptidase (MaPro 4) and one leishmanolysin-like peptidase (MaPro 14) were transcribed more than 100-folds in the cell-fed ciliates. CONCLUSIONS: The genetic information and transcriptional characteristics of the peptidases in the present results would be helpful to elucidate the role of peptidases in the invasion of scuticociliates into their hosts.
    BMC Veterinary Research 01/2013; 9(1):10. · 1.86 Impact Factor
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    ABSTRACT: Hypercholesterolemia is a key factor in the development of atherosclerosis. We sought to evaluate the relation between hypercholesterolemia and plaque composition in patients with coronary artery disease. Study subjects consisted of 323 patients (mean 61.5 years, 226 males) who underwent coronary angiography and virtual histology-intravascular ultrasound examination. Patients were divided into two groups according to total cholesterol level: hypercholesterolemic group (≥200 mg/dL, n=114) and normocholesterolemic group (<200 mg/dL, n=209). Hypercholesterolemic patients were younger (59.7±13.3 years vs. 62.6±11.5 years, p=0.036), than normocholesterolemic patients, whereas there were no significant differences in other demographics. Hypercholesterolemic patients had higher corrected necrotic core volume (1.23±0.85 mm(3)/mm vs. 1.02±0.80 mm(3)/mm, p=0.029) as well as percent necrotic core volume (20.5±8.5% vs. 18.0±9.2%, p=0.016) than normocholesterolemic patients. At the minimal lumen area site, percent necrotic core area (21.4±10.5% vs. 18.4±11.3%, p=0.019) and necrotic core area (1.63±1.09 mm(2) vs. 1.40±1.20 mm(2), p=0.088) were also higher than normocholesterolemic patients. Multivariate linear regression analysis showed that total cholesterol level was an independent factor of percent necrotic core volume in the culprit lesion after being adjusted with age, high density lipoprotein-cholesterol , hypertension, diabetes mellitus, smoking and acute coronary syndrome (beta 0.027, 95% confidence interval 0.02-0.053, p=0.037). Hypercholesterolemia was associated with increased necrotic core volume in coronary artery plaque. This study suggests that hypercholesterolemia plays a role in making plaque more complex, which is characterized by a large necrotic core, in coronary artery disease.
    Korean Circulation Journal 01/2013; 43(1):23-8.
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    ABSTRACT: Leukocytoclastic vasculitis (LCV) usually presents palpable purpura characterized by inflammation of vessel walls and fragmentation of nuclei. Various conditions can cause LCV, and it can be induced by influenza A virus infection. We report a 2-yr-old Korean girl who presented palpable purpuric and hemorrhagic lesions with fever. She was diagnosed as LCV by skin biopsy, and influenza A virus was isolated from nasopharyngeal swab. She was treated with oseltamivir (Tamiflu®) and prednisolone with dramatic effect of vasculitis and fever.
    Journal of Korean medical science 12/2012; 27(12):1601-3. · 0.84 Impact Factor
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    ABSTRACT: The relationship between the positive remodeling (PR) of a coronary artery and plaque composition has been studied only in a relatively small number of study population or non-culprit lesion. We evaluated the association between coronary plaque composition and coronary artery remodeling in a relatively large number of culprit lesions. The study population consisted of 325 consecutive patients with coronary artery disease that underwent intravascular ultrasound-virtual histology examination in a culprit lesion. The remodeling index (RI) was calculated as the lesion external elastic membrane (EEM) area divided by the average reference EEM area. The lesions with PR (RI>1.05, n=97, mean RI=1.19±0.12) had a higher fibrous volume/lesion length (3.85±2.12 mm(3)/mm vs. 3.04±1.79 mm(3)/mm, p=0.003) and necrotic core volume/lesion length (1.26±0.89 mm(3)/mm vs. 0.90±0.66 mm(3)/mm, p=0.001) than those with negative remodeling (NR) (RI<0.95, n=132, mean RI=0.82±0.09). At the minimal luminal area site, the lesions with PR had a higher fibrous area (5.81±3.17 mm(2) vs. 3.61±2.30 mm(2), p<0.001), dense calcified area (0.73±0.69 mm(2) vs. 0.46±0.43 mm(2), p=0.001), and necrotic core area (1.93±1.33 mm(2) vs. 1.06±0.91 mm(2), p<0.001) than those with NR. RI showed significant positive correlation with fibrous volume/lesion length (r=0.173, p=0.002), necrotic core volume/lesion length (r=0.188, p=0.001), fibrous area (r=0.347, p<0.001), fibrofatty area (r=0.111, p=0.036), dense calcified area (r=0.239, p<0.001), and necrotic core area (r=0.334, p<0.001). Multivariate analysis showed that the independent factor for PR was the necrotic core volume/lesion length (beta=0.130, 95% confidence interval; 0.002-0.056, p=0.037) over the entire lesion. This study suggests that PR in a culprit lesion is associated with the necrotic core volume in the entire lesion, which is a characteristic of vulnerable plaque.
    Korean Circulation Journal 11/2012; 42(11):747-52.
  • Archives of dermatology 11/2012; 148(11):1329-31. · 4.76 Impact Factor
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    ABSTRACT: The decoupling and enhancement of both Seebeck coefficient and electrical conductivity were achieved by constructing the c-axis preferentially oriented nanoscale Sb(2)Te(3) film on monolayer graphene. The external graphene layer provided a highway for charge carriers, which were stored in the thicker binary telluride film, due to the extremely high mobility.
    Physical Chemistry Chemical Physics 09/2012; 14(39):13527-31. · 4.20 Impact Factor
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    ABSTRACT: BACKGROUND: We evaluated discrepancy of calcium detection between gray scale intravascular ultrasound (IVUS) and virtual histology (VH)-IVUS and the association between coronary calcium and plaque composition. METHODS: Study population consisted of 162 consecutive patients who underwent percutaneous coronary intervention with VH-IVUS study. Subjects were divided into 3 groups based on gray scale IVUS findings; No calcification group (n=50), spotty group (n=56) who had a lesion containing only small calcium deposits within an arc <90° and diffuse group (n=56) who had a diffuse calcified lesion with an arc ≥90° in ≥1 cross-sectional image of the lesion. RESULTS: No calcification group was younger than spotty and diffuse groups (54.4±13.0years vs. 61.1±10.7years and 64.2±9.9years, p=0.011 and p<0.001, respectively). No calcification group had some degree of dense calcium (5. 7±6.9mm(3)) by VH-IVUS analysis. Furthermore, calcified volume by VH-IVUS in no calcification group was similar to those in spotty group (5.7±6.9mm(3) vs. 5.4±4.4mm(3)). Dense calcium volume was correlated directly with plaque volume (r=0.65, p<0.001), fibrous volume (r=0.54, p<0.001), fibro-fatty volume (r=0.29, p<0.001) and lipid core volume (r=0.77, p<0.001). In multiple regression analysis, lipid core volume (β=0.287, 95% confidence interval (CI) 0.187-0.388, p<0.001) was an independent predictor of dense calcium volume. CONCLUSIONS: This study showed that coronary calcium can be present even if invisible in gray scale IVUS and associated with lipid core volume, which is a characteristic of plaque vulnerability.
    International journal of cardiology 07/2012; · 6.18 Impact Factor

Publication Stats

664 Citations
179.38 Total Impact Points

Institutions

  • 2011–2014
    • Catholic University of Daegu
      Kayō, North Gyeongsang, South Korea
  • 1999–2014
    • Pukyong National University
      • • Department of Aquatic Life Medicine
      • • Department of Aquaculture
      Tsau-liang-hai, Busan, South Korea
  • 2007–2013
    • Yeungnam University
      • Department of Dermatology
      Onyang, South Chungcheong, South Korea
    • Konyang University Hospital
      Gaigeturi, Jeju, South Korea
  • 2012
    • Research Institute of Industrial Science and Technology
      Sŏul, Seoul, South Korea
    • Samsung Advanced Institute of Technology
      Usan-ri, Gyeonggi Province, South Korea
  • 2010
    • National Health Insurance Corporation Ilsan Hospital
      Sŏul, Seoul, South Korea
  • 2002–2009
    • Seoul National University
      • • Department of Materials Science and Engineering
      • • School of Electrical Engineering and Computer Sciences
      Seoul, Seoul, South Korea
  • 2003–2008
    • Yonsei University
      • • College of Medicine
      • • Department of Electrical and Electronic Engineering
      Seoul, Seoul, South Korea
  • 2006
    • Korea University
      Sŏul, Seoul, South Korea
    • Konkuk University
      • Department of Bioscience and Technology
      Sŏul, Seoul, South Korea
    • Soonchunhyang University
      • Department of Marine Biotechnology
      Asan, South Chungcheong, South Korea