Ki Hong Kim

Yonsei University Hospital, Sŏul, Seoul, South Korea

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Publications (142)260.32 Total impact

  • Yue Jai Kang, Ki Hong Kim
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    ABSTRACT: Synthetic oligodeoxynucleotides containing CpG motifs (CpG-ODNs) are potent immunostimulators in vertebrates. Based on the backbone structure and the oligonucleotides sequences, CpG-ODNs can be classified into three different classes (A, B, and C) that induce class-specific immune responses. Plasmids with stimulatory CpG motifs in their backbone also show immunostimulatory effects, and a plasmid-based delivery of CpG motifs can be an alternative approach to expensive CpG-ODNs. In this study, we constructed a plasmid (pL-CpGmix) that harbors multiple copies of CpG-ODN 2216 (class A), 1668 (class B), and 2395 (class C) motifs, and evaluated the potential of the constructed plasmid to protect of olive flounder (Paralichthys olivaceus) from viral hemorrhagic septicemia virus (VHSV) and Miamiensis avidus infections. Fish were administered pL-CpGmix, pL-GFP that harbored a green fluorescent protein (GFP) gene, empty vector, or phosphate buffered saline (PBS) alone. On 3 days post-administration, fish were challenged with VHSV or M. avidus. Fish administered pL-CpGmix showed the lowest cumulative mortality (60%) against a VHSV challenge, which was significant compared to that of the control groups (empty vector and PBS). In the M. avidus challenge experiment, fish in the group administered pCpG-mix showed the highest survival rate and serum scuticocidal activity, and those were statistically significant compared to those of control groups. Although fish administered pL-GFP showed lower survival rates than fish administered pL-CpGmix, the GFP-containing vector induced higher survival rates against M. avidus and VHSV infections and higher serum scuticocidal activity compared to the control groups, suggesting that GFP sequence might have an immunostimulating property. The present results showed that the plasmid harboring different classes of CpG motifs enhanced resistance of olive flounder against both M. avidus and VHSV infections.
    Aquaculture 09/2015; 446. DOI:10.1016/j.aquaculture.2015.05.014 · 1.83 Impact Factor
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    ABSTRACT: Several prostate-specific antigen (PSA) kinetics parameters such as nadir PSA level and time to nadir PSA level are used commonly as predictive prognostic factors for patients with metastatic prostate cancer (mPCa) who have undergone androgen deprivation therapy. However, based on the limitations of these factors, earlier and more clinically available prognostic factors are needed. Therefore, we examined the PSA half-life (PSAHL) estimated at the first follow-up visit as a prognostic factor of newly diagnosed mPCa. We performed a retrospective review of 309 patients with newly diagnosed mPCa who had undergone androgen deprivation therapy. After categorizing the included patients to short and long PSAHL groups, based on the median PSAHL value, Cox regression analyses were performed to identify independent prognostic factors of newly diagnosed mPCa. The Kaplan-Meier method was used for detecting differences in survival between both the groups. The median follow-up period was 44 months, and the prostate cancer-specific mortality (PCSM)-free survival length was 65 months in all included patients. Long PSAHL group (hazard ratio [HR] = 2.383, P<0.001), nadir PSA level (HR = 1.004, P<0.001), time to nadir PSA level (HR = 0.856; P<0.001), and Gleason score 8 to 10 relative to 6 to 7 (HR = 2.025; P = 0.008) were found to be independent predictors of the PCSM. By the Kaplan-Meier method, the median PCSM-free survival of the short PSAHL group was 73.7 (95% CI: 54.8-92.6) and of the long PSAHL group was 52.5 months (95% CI: 33.4-71.6). This difference between both the groups was found to be statistically significant (P = 0.014, log rank test). PSAHL estimated at the first follow-up visit is an independent prognostic factor for newly diagnosed mPCa. If the prospective validation test is performed on a large scale, it may demonstrate that PSAHL is an early surrogate prognostic factor of newly diagnosed mPCa. Copyright © 2015 Elsevier Inc. All rights reserved.
    Urologic Oncology 05/2015; DOI:10.1016/j.urolonc.2015.04.007 · 3.36 Impact Factor
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    ABSTRACT: Prostate-specific antigen (PSA) is a surrogate marker of disease progression; however, its predictive ability in the extreme ranges is unknown. We determined the predictors of survival in patients with bone metastatic prostate cancer (BMPCa) and with extremely high PSA levels.
    02/2015; 20(1). DOI:10.1016/j.prnil.2015.02.006
  • Yue Jai Kang, Ki Hong Kim
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    ABSTRACT: Immunotherapy along with chemotherapy can be a way to enhance therapeutic efficacy and to reduce amount of chemotherapeutics. So, in the present study, we adopted an immunochemotherapy (CpG-ODN 1668 plus doxycycline) to reduce doxycycline dose to be used while at the same time to get the same treatment efficacy against scuticociliatosis in olive flounder (Paralichthys olivaceus). The survival rates of fish administered with doxycycline 100 mg/kg plus 10 μg of CpG-ODN 1668 were higher than those of fish administered with doxycycline alone at a dose of 200 mg/kg of fish weight, suggesting that coadministration of CpG-ODN 1668 can reduce doxycycline to half the dose. Furthermore, reduction of CpG-ODN 1668 to 5 μg/fish plus doxycycline 100 mg/kg led to high survival rates that were comparable to doxycycline 200 mg/kg, suggesting that the used amount of both immunotherapeutics and chemotherapeutics can be reduced through coadministration. There have been no reports on the treatment of scuticociliatosis by combining chemotherapy and immunotherapy. In the present study, we demonstrated that a combination of chemotherapy (doxycycline) and immunotherapy (CpG-ODN 1668) was effective against scuticociliatosis in olive flounder fingerlings.
    Aquaculture 01/2015; 435:143–145. DOI:10.1016/j.aquaculture.2014.09.024 · 1.83 Impact Factor
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    ABSTRACT: To determine the prognostic impact of synchronous second primary malignancy (SPM) on the overall survival (OS) of patients with metastatic prostate cancer (mPCa), as identifying features that stratify the risk of OS is critical for judicious application of a definitive therapy. A retrospective analysis was conducted on 582 consecutive PCa patients diagnosed with metastasis between May 7, 1998 and August 27, 2011. Patient age, body mass index, Eastern Cooperative Oncology Group performance, Charlson comorbidity index, prostate-specific antigen, T and N stages, Gleason score, American Society of Anesthesiologists (ASA) score, progression to castration-resistant PCa, prior local treatments, and the presence of SPMs at the time of metastasis were assessed. SPM was defined as a cytologically or histologically proven solid malignancy. Cox-proportional hazards regression analyses were used to estimate OS according to the type of SPM and to evaluate predictive variables. A total of 164 (28.1%) patients had synchronous SPM, of which colorectal (9.1%), stomach (7.3%), and lung (7.1%) cancers were the most prevalent. During a median follow-up of 34.1 months, patients without SPM had significantly higher OS rates than men with lung and stomach cancers; however, they had a comparable outcome to men with colorectal cancer. Clinical stage ≥T4, ASA score ≥1, and the presence of lung or stomach cancer were independent predictors of overall mortality. A substantial proportion of patients with mPCa present with synchronous SPM. Definitive therapy targeting PCa may confer limited survival benefits for patients with synchronous lung or stomach cancer. Copyright © 2014 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.
    The Journal of Urology 10/2014; 193(4). DOI:10.1016/j.juro.2014.10.088 · 3.75 Impact Factor
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    Ki Hong Kim
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    ABSTRACT: Clival chordoma is a rare intracranial neoplasm located in the clivus with bony extension and destruction. It is difficult to resect completely and generally has a poor prognosis. However, intradural clival chordomas have been reported with good surgical outcomes. We present a rare case of intradural chordoma and a review of the literature.
    10/2014; 2(2):76-80. DOI:10.14791/btrt.2014.2.2.76
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    ABSTRACT: Previously generated two auxotrophic genes knockout Edwardsiella tarda (Δalr Δasd E. tarda) was used as a delivery vehicle for CpG 1668 motif-enriched plasmids (pL-CpG30), and potential of the Δalr Δasd E. tarda-mediated delivery of CpG motifs in both prevention and therapeutic treatment of scuticociliatosis caused by Miamiensis avidus in olive flounder (Paralichthys olivaceus) was investigated. The auxotrophic E. tarda mutant with pL-CpG30 plasmids elicited evidently higher survival rates and delayed both onset of mortality and time-to-death of olive flounder against M. avidus challenge. Furthermore, administration of E. tarda mutant that contains plasmids enriched in CpG 1668 motif elicited clearly higher survival rates of fish that were already infected with M. avidus. These results indicate that CpG 1668 plasmids-harboring E. tarda mutant may provide not only preventive measure but also therapeutic approach against scuticociliatosis in olive flounder.
    Experimental Parasitology 06/2014; DOI:10.1016/j.exppara.2014.06.007 · 1.86 Impact Factor
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    ABSTRACT: Hydranencephaly is a rare congenital disease defined as an absence of cerebral hemispheres with an intact thalamus, brain stem, and cerebellum. Generally, patients with hydranencephaly require cerebro-spinal fluid diversion due to progressive hydrocephalus. An excellent view of the choroid plexus and advancement of the neurosurgical endoscope were possible, leading to easy coagulation of the choroid plexus to balance cerebro-spinal fluid production without the use of a shunt device. We present this rare case and good treatment outcome from endoscopic coagulation of the choroid plexus.
    Journal of Korean Neurosurgical Society 06/2014; 55(6):375-8. DOI:10.3340/jkns.2014.55.6.375 · 0.52 Impact Factor
  • Yue Jai Kang, Ki Hong Kim
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    ABSTRACT: Scuticociliatosis caused by Miamiensis avidus is one of the major diseases in cultured olive flounder (Paralichthys olivaceus) in Korea. However, until recently, no effective chemotherapy or vaccine has been developed. Oligodeoxynucleotides containing unmethylated deoxycytidyl-deoxyguanosine motifs (CpG-ODNs) have been shown to be effective in enhancing innate immune responses and preventing infectious diseases in fish. However, it has not been experimented whether CpG-ODNs can be used as a therapeutic tool against infectious diseases in fish. In the present study, we evaluated the therapeutic potential of CpG-ODN 1668 against scuticociliatosis in olive flounder for the first time. The group of fish administered CpG-ODN 1668 at 3 days after the infection showed clearly higher survival rates than the groups of fish administered GpC-ODN 1720 or PBS, indicating a high therapeutic potential of CpG-ODN 1668 against scuticociliatosis. However, the treatment efficacy of CpG-ODN 1668 was diminished when administered at 6 days post-infection, and no treatment effect was found when administered at 9 days after the infection. These results suggest that early diagnosis through continuous monitoring and early treatment with CpG-ODN 1668 are essential for increase of treatment efficacy. In conclusion, the present study demonstrated for the first time the possible use of CpG-ODN 1668 in treatment of scuticociliatosis in olive flounder.
    Aquaculture 06/2014; 430:17–20. DOI:10.1016/j.aquaculture.2014.03.035 · 1.83 Impact Factor
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    ABSTRACT: The resistance against white spot syndrome virus (WSSV) infection in wild marine crab Gaetice depressus by the immunization of a recombinant glutathione-S-transferase (GST) fused VP28 protein (GST-VP28) was evaluated. The cumulative mortalities of GST-VP28 injected groups were lower than those of the control groups at 10 days of post-challenge, and the time to death of 50% crab () was delayed by the immunization using GST-VP28. The group boosted with GST-VP28 after 2 weeks of primary immunization clearly showed longer than non-boosted group against challenge with WSSV. This result suggests that boosting with the antigen protein elicit stronger immune responses similar to adaptive immune responses of vertebrates. However, the short was observed in the group challenged at 3 weeks post boosting comparing to the group challenged at 1 week post boosting. This suggests that the protective strength of immunization decreased by the time.
    04/2014; 27(1). DOI:10.7847/jfp.2014.27.1.011
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    ABSTRACT: Luteolin, a flavonoid extracted from a number of plants with recognized anticancer, anti-inflammatory and anti-oxidative activities, inhibits angiogenic processes and modulates multidrug resistance. However, the efficacy and mechanisms of action of this flavonoid agent are still undergoing study. In order to elucidate whether luteolin exhibits an anticancer effect in cervical cancer cells, HeLa cells were incubated with luteolin and apoptosis was assessed by observing nuclear morphological changes, and performing Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining. Cell cycle analysis, western blotting, RT-PCR and mitochondrial membrane potential measurements were also carried out. Luteolin showed a significant dose-dependent cytotoxic effect only in human papillomavirus (HPV)-positive cervical cancer cells, when compared to its effect on HPV-negative cervical cancer C33A cells. Expression levels of human papilloma virus E6 and E7 oncogenes were suppressed, those of related factors pRb and p53 were recovered and E2F5 was increased by luteolin treatment. Furthermore, luteolin enhanced the expression of death receptors and death receptor downstream factors such as Fas/FasL, DR5/TRAIL and FADD in HeLa cells, and activated caspase cascades. In particular, luteolin enhanced the activity of caspase-3 and -8 in a dose-dependent manner. Activation of caspase-3 induced caspase-8 activity and vice versa. Luteolin also induced mitochondrial membrane potential collapse and cytochrome c release, and inhibited Bcl-2 and Bcl-xL expression. In conclusion, luteolin exerts anticarcinogenic activity through inhibition of E6 and E7 expression and cross-activation of caspase-3 and -8. Taken together, these results suggest that luteolin induces inactivation of HPV-18 oncogene expression and apoptosis by activating the intrinsic and extrinsic pathways.
    Oncology Reports 04/2014; 31(6). DOI:10.3892/or.2014.3157 · 2.19 Impact Factor
  • Yue Jai Kang, Dong Soo Kim, Ki Hong Kim
    Aquaculture Research 04/2014; 45(5). DOI:10.1111/are.12020 · 1.32 Impact Factor
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    ABSTRACT: Protection of olive flounder (Paralichthys olivaceus) from virulent Edwardsiella tarda infection by oral immunization with two auxotrophic genes – alanine racemase gene (alr) and aspartate semialdehyde dehydrogenase (asd) gene – knock-out E. tarda mutant (ΔalrΔasd E. tarda) was previously demonstrated. In the present study, we investigated the effects of cimetidine, a histamine2 (H2) receptor antagonist, on the arrival of the live auxotrophic E. tarda mutant to the intestine of olive flounder and on the protective efficacy of the live ΔalrΔasd E. tarda oral vaccine. In the experiment to determine optimal administration time of cimetidine, fish administered cimetidine at 3 h before the administration of the E. tarda mutant showed the highest bacterial colony number from the intestine. In the experiment to determine optimal dose of cimetidine, the groups of fish administered 100 mg or 200 mg of cimetidine per kg of fish showed significantly higher CFU numbers in the intestine compared to the group intubated with cimetidine 10 mg or 50 mg per kg of fish. However, as there was no significant difference between cimetidine 100 mg and 200 mg groups, a dose of 100 mg per kg of fish was chosen as the optimal dose of cimetidine. In the oral vaccine efficacy experiment, the group of fish administered the live auxotrophic E. tarda mutant in combination with the above optimal regime of cimetidine showed the highest serum agglutination activity and survival rates, suggesting that cimetidine can be used to enhance the protective efficacy of the oral live bacterial vaccines in fish. To our knowledge, this is the first study that demonstrated cimetidine-mediated enhancement of protective efficacy in oral vaccine of fish.
    Aquaculture 01/2014; s 420–421:206–210. DOI:10.1016/j.aquaculture.2013.11.015 · 1.83 Impact Factor
  • Yue Jai Kang, Dong Soo Kim, Ki Hong Kim
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    ABSTRACT: Scuticociliatosis is a leading cause of mass mortality in cultured olive flounder (Paralichthys olivaceus) in Korea. However, still no effective treatment measures have been developed. In the present study, we selected two drugs, doxycycline and albendazole, that were demonstrated to have high in vitro scuticocidal activity and possess ability to cross blood-brain barrier (BBB), and evaluated their treatment efficacy against scuticociliatosis caused by Miamiensis avidus in olive flounder. In both oral and bath treatment experiments, groups of fish fed a diet containing doxycycline or bathed with freshwater containing doxycycline showed clearly higher survival rates than control groups. Although the groups of fish fed a diet containing albendazole or bathed with freshwater containing albendazole showed higher survival rates than the control group, the rates were greatly lower than those of the groups treated with doxycycline. Poor intestinal absorption, low solubility in water, and low lipophilicity of albendazole were considered as the possible cause of the low efficacy. In conclusion, the present results suggest that doxycycline can be used to treat systemic scuticociliatosis in olive flounder through oral administration and bath.
    Aquaculture 12/2013; 416-417:192-195. DOI:10.1016/j.aquaculture.2013.09.017 · 1.83 Impact Factor
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    ABSTRACT: The differential diagnosis of bluish-gray pigmentation of the skin includes dermal melanocytosis, malignant melanoma and metallic discoloration. Of these disorders, metallic discoloration, which develops from deposits of fine metallic particles on the skin, is an uncommon dermatologic disease. We report a case of metallic discoloration of the right shin caused by titanium alloy prostheses in a patient with right total knee replacement. The patient had bluish-gray pigmentation on her right shin as well as pain while walking. Histopathologic findings of the skin lesion showed abundant black particles in the perivascular area of the lower dermis. Polarizing microscopy revealed refractile foreign materials in the dermis. An energy dispersive X-ray spectrophotometer unit attached to a transmission electron microscope detected titanium and aluminium peaks in the specimen.
    Annals of Dermatology 08/2013; 25(3):356-9. DOI:10.5021/ad.2013.25.3.356 · 0.95 Impact Factor
  • 06/2013; 16(2):131-135. DOI:10.5657/FAS.2013.0131
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    ABSTRACT: It is unclear which plaque component is related with long-term clinical outcomes in patients with coronary artery occlusive disease (CAOD). We assessed the relationship between plaque compositions and long-term clinical outcomes in those patients. The study subjects consisted of 339 consecutive patients (mean 61.7±12.2 years old, 239 males) who underwent coronary angiogram and a virtual histology-intravascular ultrasound examination. Major adverse cardiac and cerebrovascular events (MACCE), including all-cause death, non-fatal myocardial infarction, cerebrovascular events, and target vessel revascularization were evaluated during a mean 28-month follow-up period. Patients with high fibrofatty volume (FFV, >8.90 mm(3), n=169) had a higher incidence of MACCE (25.4% vs. 14.7%, p=0.015), male sex (75.7% vs. 65.3%, p=0.043), acute coronary syndrome (53.3% vs. 35.9%, p=0.002), multivessel disease (62.7% vs. 41.8%, p<0.001) and post-stent slow flow (10.7% vs. 2.4%, p=0.002) than those with low FFV (FFV≤8.90 mm(3), n=170). Other plaque composition factors such as fibrous area/volume, dense calcified area/volume, and necrotic core area/volume did not show any impact on MACCE. Cardiogenic shock {hazard ratio (HR)=8.44; 95% confidence interval (CI)=3.00-23.79; p<0.001} and FFV (HR=1.85; 95% CI=1.12-3.07; p=0.016) were the independent predictors of MACCE by Cox regression analysis. Thin-cap fibroatheroma, necrotic core area, and necrotic core volume were not associated with MACCE. FFV of a culprit lesion was associated with unfavorable long-term clinical outcomes in patients with CAOD.
    Korean Circulation Journal 06/2013; 43(6):377-83. DOI:10.4070/kcj.2013.43.6.377
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    ABSTRACT: In mammals, hypoxia-inducible factor-1 α (HIF-1α) is known to play important roles not only in oxygen homeostasis but also in innate immune responses. In this study, to assess the functional role of HIF-α in respiratory burst activity of Crassostrea gigas hemocytes, oysters were injected with HIF-α- or green fluorescent protein (GFP)-targeted-long double-stranded RNAs (dsRNAs), and at 1, 3, and 7 days post-injection, knock-down of C. gigas HIF-α expression and production of reactive oxygen species (ROS) were analyzed. Expression of HIF-α in mantle, gill, and hemocytes of C. gigas was clearly down-regulated by injection of the HIF-α-targeted-long dsRNA, but was not inhibited by the GFP-targeted-long dsRNA, indicating that HIF-α expression was suppressed through sequence-specific and systemic RNA interference (RNAi). Respiratory burst activity of hemocytes was significantly increased by administration of GFP-targeted-long dsRNA. However, knock-down of HIF-α expression led to significant decrease of chemiluminescence (CL) response of C. gigas hemocytes at 3 and 7 d post-administration of HIF-α-targeted-long dsRNA, indicating the critical role of HIF-α in activation of respiratory burst activity of oyster hemocytes.
    Fish &amp Shellfish Immunology 05/2013; DOI:10.1016/j.fsi.2013.05.001 · 3.03 Impact Factor

Publication Stats

1k Citations
260.32 Total Impact Points


  • 2008–2015
    • Yonsei University Hospital
      • • Department of Internal Medicine
      • • Surgery
      Sŏul, Seoul, South Korea
  • 1997–2015
    • Pukyong National University
      • • Department of Aquatic Life Medicine
      • • College of Fisheries Science
      Tsau-liang-hai, Busan, South Korea
  • 2011–2014
    • Catholic University of Daegu
      Kayō, North Gyeongsang, South Korea
  • 2006–2014
    • Konkuk University
      • Department of Bioscience and Technology
      Sŏul, Seoul, South Korea
    • Korea University
      Sŏul, Seoul, South Korea
  • 2007–2013
    • Yeungnam University
      • Department of Dermatology
      Gyeongsan, Gyeongsangbuk-do, South Korea
    • Konyang University Hospital
      Gaigeturi, Jeju, South Korea
    • Korea Atomic Energy Research Institute (KAERI)
      Daiden, Daejeon, South Korea
  • 2012
    • Konyang University
      Ronsan, Chungcheongnam-do, South Korea
    • Research Institute of Industrial Science and Technology
      Sŏul, Seoul, South Korea
  • 2011–2012
    • Hanyang University
      Sŏul, Seoul, South Korea
    • Samsung Advanced Institute of Technology
      Usan-ri, Gyeonggi Province, South Korea
  • 2010
    • National Health Insurance Corporation Ilsan Hospital
      Sŏul, Seoul, South Korea
  • 2002–2009
    • Seoul National University
      • • Department of Materials Science and Engineering
      • • Department of Industrial Engineering
      • • School of Electrical Engineering and Computer Sciences
      Sŏul, Seoul, South Korea
  • 2003
    • Yonsei University
      • Department of Electrical and Electronic Engineering
      Seoul, Seoul, South Korea
  • 1987
    • Hanyang University Medical Center
      Sŏul, Seoul, South Korea