Jing Li

Chinese Academy of Sciences, Peping, Beijing, China

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Publications (51)84.83 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Nickel pollution imposes deleterious effects on soil ecosystem. The responses of soil microorganisms to long-term nickel pollution under field conditions remain largely unknown. Here, we used high-throughput sequencing to elucidate the impacts of long-term nickel pollution on soil bacterial communities in two contrasting agricultural soils. Our results found that the soil microbial biomass carbon consistently decreased along the nickel gradients in both soils. Nickel pollution selectively favored or impeded the prevalence of several dominant bacterial guilds, in particular, Actinobacteria showed tolerance, while Acidobacteria and Planctomycetes displayed sensitivity. Despite the apparent shifts in the bacterial community composition, no clear tendency in the bacterial diversity and abundance was identified along the nickel gradients in either soil. Collectively, we provide evidence that long-term nickel pollution shifted the soil bacterial communities, resulting in the decrease of microbial biomass although the bacterial diversity was not significantly changed.
    Environmental Science and Pollution Research 03/2015; DOI:10.1007/s11356-015-4232-1
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    ABSTRACT: Copper contamination on China's arable land could pose severe economic, ecological and healthy consequences in the coming decades. As the drivers in maintaining ecosystem functioning, the responses of soil microorganisms to long-term copper contamination in different soil ecosystems are still debated. This study investigated the impacts of copper gradients on soil bacterial communities in two agricultural fields with contrasting soil properties. Our results revealed consistent reduction in soil microbial biomass carbon (SMBC) with increasing copper levels in both soils, coupled by significant declines in bacterial abundance in most cases. Despite of contrasting bacterial community structures between the two soils, the bacterial diversity in the copper-contaminated soils showed considerably decreasing patterns when copper levels elevated. High-throughput sequencing revealed copper selection for major bacterial guilds, in particular, Actinobacteria showed tolerance, while Acidobacteria and Chloroflexi were highly sensitive to copper. The thresholds that bacterial communities changed sharply were 800 and 200 added copper mg kg(-1) in the fluvo-aquic soil and red soil, respectively, which were similar to the toxicity thresholds (EC50 values) characterized by SMBC. Structural equation model (SEM) analysis ascertained that the shifts of bacterial community composition and diversity were closely related with the changes of SMBC in both soils. Our results provide field-based evidence that copper contamination exhibits consistently negative impacts on soil bacterial communities, and the shifts of bacterial communities could have largely determined the variations of the microbial biomass.
    Frontiers in Microbiology 02/2015; 6:31. DOI:10.3389/fmicb.2015.00031
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    ABSTRACT: Purpose Despite their symbiotic relationship with trees and the vital role as decomposer in forest, soil fungi received limited attention regarding their changes with altitude in forest ecosystems. This study aimed to determine the diversity patterns of soil fungi along an altitudinal gradient on Mt. Shegyla, a typical forest ecosystem on the Tibetan Plateau. Materials and methods High-throughput barcoded pyrosequencing and quantitative PCR approaches were employed to measure the community composition, diversity, and abundance patterns of soil fungal 18S ribosomal RNA (rRNA) gene in 20 samples collected along the altitudinal gradient of Mt. Shegyla. Results and discussion Abundant taxa in the fungal community were Agaricomycetes and Leotiomyceta on Mt. Shegyla. Fungal abundance decreased significantly with increasing altitude. Beta diversity of the fungal community, as measured using weighted UniFrac distance, was significantly related to altitude. Significant correlation was observed between altitude and alpha diversity including richness and phylodiversity, but not with evenness. Network analysis revealed that Ceramothyrium and Clavulina were two important hubs in the community, and an uncultured fungal taxon that previously detected in glacier forefront dominated this network. Distance-based linear model identified soil pH as the dominant driver which significantly related with fungal alpha diversity including richness, phylodiversity, and evenness. However, fungal abundance and the first component of PCoA on weighted UniFrac matrix (beta diversity) did not change significantly with pH. Conclusions These results provided strong evidence that soil pH was the dominant driver for structuring altitudinal alpha diversity pattern but not beta diversity pattern or community abundance of soil fungi in this typical forest on the Tibetan Plateau.
    Journal of Soils and Sediments 01/2015; DOI:10.1007/s11368-015-1070-1
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    ABSTRACT: Metal aging in soils has been considered an important factor influencing its availability and toxicity to organisms. In this study, we report the influence of 5 years field aging on the nickel (Ni) toxicity to collembolan Folsomia candida based on two different types of soil from Dezhou (DZ) and Qiyang (QY) counties in China. Acute and chronic toxicity of Ni to F. candida was assessed in both freshly spiked and field aging contaminated soils. We found that 5 years field aging increased the EC50 and 2d-LC50 values of Ni to F. candida in the DZ soil, while little influence on the Ni toxicity was observed in the QY soil. There was no adverse effect of the long-term field aging on the Ni toxicity to the survival of F. candida in the two tested soils. In addition, field aging of the two soils impacted differently the water-soluble Ni concentrations, which were significantly correlated to the juvenile production of F. candida based on a logistic model. Our study highlights different effects of long-term field aging on the Ni toxicity to F. candida between divergent types of soil, and this should be taken into account in future toxicity testing and risk assessment practices.
    Environmental Science and Pollution Research 12/2014; 22(11). DOI:10.1007/s11356-014-3968-3
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    ABSTRACT: Terahertz (THz) frequency region, defined from 0.1 to 10 THz, is an important frequency band for radio astronomy and atmospheric science. As NbN Superconductor-Insulator-Superconductor (SIS) mixers used for terahertz detection, which are studied by the Purple Mountain Observatory (PMO), Chinese Academy of Sciences (CAS), work at 8–10 K, and require condition of micro vibrations, its astronomical observation in aerospace is limited by suitable refrigeration method. 4 K high frequency pulse tube cryocooler developed by Key Laboratory of Space Energy Conversion Technologies (SECT), Technical Institute of Physics and Chemistry (TIPC), CAS, offers an opportunity for the application of SIS mixers. This article introduces the progress of the two-stage high frequency pulse tube cryocooler researched by TIPC. The cryocooler has reached a no load temperature of 4.5 K which is the lowest temperature for this kind of cryocooler reported so far. The successful coupling between the THz component and the high frequency pulse tube cryocooler lays a solid foundation for space detection in the terahertz band.
    Chinese Science Bulletin 09/2014; 59(27). DOI:10.1007/s11434-014-0457-5
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    ABSTRACT: Unraveling the distribution patterns of plants and animals along the elevational gradients has been attracting growing scientific interests of ecologists, whether the microbial communities exhibit similar elevational patterns, however, remains largely less documented. Here, we investigate the biogeographic distribution of soil archaeal and bacterial communities across three vertical climate zones (3,106-4,479 m.a.s.l.) in Mt. Shegyla on the Tibetan Plateau, by combining quantitative PCR and high-throughput barcoded pyrosequencing approaches. Our results found that the ratio of bacterial to archaeal 16S rRNA gene abundance was negatively related with elevation. Acidobacteria dominated in the bacterial communities, Marine benthic group A dominated in the archaeal communities, and the relative abundance of both taxa changed significantly with elevation. At the taxonomic levels of domain, phylum, and class, more bacterial taxa than archaeal exhibited declining trend in diversity along the increasing elevational gradient, as revealed by Shannon and Faith's phylogenetic diversity indices. Unweighted UniFrac distance clustering showed that the bacterial communities from the mountainous temperate zone clustered together, whereas those from the subalpine cool temperate zone clustered together. However, the partitioning effect of elevational zones on the archaeal community was much weaker compared to that on bacteria. Redundancy analysis revealed that soil geochemical factors explained 58.3 % of the bacterial community variance and 75.4 % of the archaeal community variance. Taken together, we provide evidence that soil bacteria exhibited more apparent elevational zonation feature and decreased diversity pattern than archaea with increasing elevation, and distribution patterns of soil microbes are strongly regulated by soil properties along elevational gradient in this plateau montane ecosystem.
    Microbial Ecology 07/2014; 69(1). DOI:10.1007/s00248-014-0465-7
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    ABSTRACT: Niobium nitride (NbN) superconducting tunnel junctions (STJs) have an energy gap nearly double that of niobium STJs, making them potential sensitive detectors that operate at higher frequencies and temperatures. In this paper, we investigate the direct-detection behavior (i.e. the spectral response, current responsivity, noise characteristics, and noise equivalent power (NEP)) of a 500 GHz NbN STJ detector at temperatures from approximately 5 to 9 K. The detector shows an uncorrected NEP of 3.8 × 10−13 W/around 5 K and 1.5 × 10−12 W/at 9 K. Its performance can be further improved by adopting a cryogenically cooled readout circuit and fabricating the device with a wiring layer of higher critical-transition temperatures.
    Superconductor Science and Technology 05/2014; 27(7):075003. DOI:10.1088/0953-2048/27/7/075003
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    ABSTRACT: To improve the prediction of essential ecosystem functioning under future environmental disturbances, it is of significance to identify responses of soil microorganisms to environmental stresses. In this study, we collected polluted soil samples from field plots with eight copper levels ranging from 0 to 3,200 mg Cu kg(-1) soil. Then, the soils with 0 and 3,200 mg Cu kg(-1) were selected to construct a microcosm experiment. Four treatments were set up including Cu0-C and Cu3200-C without further Cu addition, and Cu0-A and Cu3200-A with addition of 57.5 mg Cu kg(-1) soil. We measured substrate-induced respiration (SIR) and potential nitrification rate (PNR). Furthermore, the abundance of bacterial, archaeal 16S rRNA genes, ammonia-oxidizing bacteria and archaea amoA genes were determined through quantitative PCR. The soil microbial communities were investigated by terminal restriction fragment length polymorphism (T-RFLP). For the field samples, the SIR and PNR as well as the abundance of soil microorganisms varied significantly between eight copper levels. Soil microbial communities highly differed between the low and high copper stress. In the microcosm experiment, the PNR and SIR both recovered while the abundance of soil microorganisms varied irregularly during the 90-day incubation. The differences of microbial communities measured by pairwise Bray-Curtis dissimilarities between Cu0-A and Cu0-C on day 0 were significantly higher after subsequent stress than before. However, the differences of microbial communities between Cu3200-A and Cu3200-C on day 0 changed little between after subsequent stress and before. Therefore, initial copper stress could increase the resistance of soil microorganisms to subsequent copper stress.
    Microbial Ecology 02/2014; 67(4). DOI:10.1007/s00248-014-0391-8
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    ABSTRACT: Terahertz Superconducting Imaging Array (TeSIA) is a project for the development of a large THz direct-detection array for mapping observations. The prototype of TeSIA is an 8×8 pixel direct-detection array operating at the 850 μm band, incorporating THz superconducting detectors such as kinetic inductance detectors (KIDs) and transition edge sensors (TES). KIDs have the advantage that only a broadband low noise cryogenic amplifier is needed at low temperature for the readout system, and hundreds of pixels could be readout simultaneously by the frequency-division multiplexing (FDM) technique. The readout system for a KIDs array is composed of several parts like excitation signal generating, intermediate frequency (IF) circuit, baseband signal acquisition and processing. Excitation signal is a kind of comb signal carrying various frequencies corresponding simply to resonant frequencies of the detectors. It is generated in baseband with a bandwidth covering all KIDs and up-converted to microwave frequency (about several gigahertz) to feed the detectors. With THz radiation, the forward transmission coefficient (S21) of all KIDs varies and the variation can be measured through the comb signal. Fast Fourier transform (FFT) with pipeline structure will be used to process the baseband excitation signal in real time. Consequently, the radiation intensity can be estimated by monitoring the signal amplitude and phase of the corresponding frequency channels. In this paper we will present mainly the design ofan electronic readout system for the 8×8 pixel array.
    Proceedings of SPIE - The International Society for Optical Engineering 12/2012; DOI:10.1117/12.999786
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    ABSTRACT: In this paper, we will introduce a dual-THz-band SIS (Superconductor-Insulator-Superconductor) heterodyne radiometer system developed for the atmospheric profiling synthetic observation system project (APSOS). This THz system is intended to have a durable and compact design to meet the challenging requirements of remote operation. The system as well as its major components such as antenna tipping, quasi-optics, cryogenics, SIS mixers and FFTS backend will be discussed thoroughly. Some scientific simulation focusing on the atmospheric profiling components at THz bands will also be investigated.
    Proceedings of SPIE - The International Society for Optical Engineering 12/2012; DOI:10.1117/12.999736
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    ABSTRACT: Superconducting tunnel junctions are good candidates for the direct detection of terahertz signals. In this paper, the direct detection behavior of Nb-based superconducting tunnel junctions at 493GHz is demonstrated. The performance of the detector is evaluated in several aspects, including noise characteristics, current response and frequency response.
    2012 International Conference on Microwave and Millimeter Wave Technology (ICMMT); 05/2012
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    ABSTRACT: To understand concentrations of perfluorinated compounds (PFCs) in aquatic products, and risks to human health, concentrations of 12 PFCs were measured in edible tissues of aquatic products collected from Tianjin, China, a typical industrial area on the western Bohai Sea. Perfluorooctane sulfonate (PFOS) was the predominant PFC. Concentrations of PFOS in aquatic products from Tianjin Binhai New Area (TBNA) ranged from
    Human and Ecological Risk Assessment 11/2011; 17(6):1279-1291. DOI:10.1080/10807039.2011.618395
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    ABSTRACT: Concentrations of metals and arsenic in farmland soils along the Yanghe River, upstream of Guanting Reservoir, were evaluated using fuzzy comprehensive assessment (FCA). FCA is an emerging methodology for assessing environmental status based on fuzzy logic which can model linguistic data and represent chaotic conditions. The ratio of concentrations of metals and arsenic (As) in surface soils of farmland along the Yanghe River to the corresponding reference values decreased in the order Cd>Zn>Cr>Ni>Pb>Cu>As. Based on the FCA, 86% of surface soils along the Yanghe River were classified as grade C, which is suitable for forestry with a greater absorption capacity, suggesting that most farmland along the Yanghe River has to be abandoned as farmland. In order to improve the quality of soil along the Yanghe River and avoid degeneration of water quality in the river and the Guanting Reservoir, remediation of farmland soils along the river is suggested.
    Chemistry and Ecology 10/2011; 27(5):415-426. DOI:10.1080/02757540.2011.595711
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    ABSTRACT: Epigenetic modifications are thought to be important for gene expression changes during development and aging. However, besides the Sir2 histone deacetylase in somatic tissues and H3K4 trimethylation in germlines, there is scant evidence implicating epigenetic regulations in aging. The insulin/IGF-1 signaling (IIS) pathway is a major life span regulatory pathway. Here, we show that progressive increases in gene expression and loss of H3K27me3 on IIS components are due, at least in part, to increased activity of the H3K27 demethylase UTX-1 during aging. RNAi of the utx-1 gene extended the mean life span of C. elegans by ~30%, dependent on DAF-16 activity and not additive in daf-2 mutants. The loss of utx-1 increased H3K27me3 on the Igf1r/daf-2 gene and decreased IIS activity, leading to a more "naive" epigenetic state. Like stem cell reprogramming, our results suggest that reestablishment of epigenetic marks lost during aging might help "reset" the developmental age of animal cells.
    Cell metabolism 08/2011; 14(2):161-72. DOI:10.1016/j.cmet.2011.07.001
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    ABSTRACT: Twin SIS (superconductor-insulator-superconductor) junctions, with a simple structure, yet large bandwidth and low noise temperature, have been widely used in millimeter- and submillimeter-wave heterodyne mixers. With increasing frequency, however, the length of the tuning inductance connecting the two individual SIS junctions becomes short while the junction area remains fixed. With a relatively short tuning inductance, the effect of the junction's spreading inductance becomes non-negligible, and device fabrication becomes more difficult. By adopting vertically stacked SIS junctions (VSJs), which have an equivalent geometric capacitance inversely proportional to the junction number, it becomes feasible to increase the junction tuning inductance. In this paper, we report on the design, fabrication, and characterization of twin Nb/Al-AlOx/Nb/Al-AlOx/Nb VSJs for the 500-GHz frequency band.
    IEEE Transactions on Applied Superconductivity 07/2011; 21(3-21):663 - 666. DOI:10.1109/TASC.2010.2096493
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    ABSTRACT: Concentrations of perfluorooctanesulfonate (PFOS), perfluorooctanoic acid (PFOA) and other perfluorinated compounds (PFCs) were measured in water and sediment from coastal Bohai Bay and surrounding rivers flowing into the bay. Of the 15 PFCs measured, PFOS and PFOA were detected with the greatest frequency. Concentrations in water ranged from<0.2 to 31 ng·L−1 and<1.0 to 82 ng·L−1 for PFOS and PFOA, respectively. Concentrations of PFOS and PFOA in sediments ranged from<0.1 to 2.0 ng·g−1 dw and<0.1 to 0.5 ng·g−1 dw, respectively. Concentrations of PFCs in Bohai Bay were less than those observed in other areas in Asia, but greater concentrations of ∑PFCs were observed in the Dalin River with concentrations increasing from upstream to downstream, and the greatest concentrations in sediment were observed in tidal flats. The ratio of ∑PFCs in sediment and water indicated that sediment could serve as a significant sink for PFUnA.
    Chemistry and Ecology 04/2011; 27(2-2):165-176. DOI:10.1080/02757540.2010.547485
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    ABSTRACT: A case study of the cancer risk to humans posed by persistent organic pollutants (POPs) in an industrial area of China, which has a long history of contamination from many sources, is presented. Relatively great concentrations of POPs around the chemical industrial parks have the potential to be chronically carcinogenic to local people. Sixteen individual PAHs listed for priory control by the U.S. Environmental Protection Agency (USEPA), metabolites of DDTs, and isomers of HCHs were measured in soils and a human health risk assessment was conducted by use of USEPA exposure models for children and adults, respectively. Geostatistical methods were used to simulate the spatial diffusion of potential carcinogenic risk, and non-parametric Mann-Whitney U and Kruskal-Wallis tests were employed to analyze the impact of point sources on the surrounding area. The mean value of the sum of Excess Lifetime Cancer Risk (Sigma ELCR) exceeded the generally acceptable risk level of 1.0E-06 recommended by the USEPA for
    Human and Ecological Risk Assessment 10/2010; 16. DOI:10.1080/10807039.2010.512238
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    ABSTRACT: Rhesus macaque is a widely used primate model organism. Its genome annotations are however still largely comparative computational predictions derived mainly from human genes, which precludes studies on the macaque-specific genes, gene isoforms or their regulations. Here we took advantage of histone H3 lysine 4 trimethylation (H3K4me3)'s ability to mark transcription start sites (TSSs) and the recently developed ChIP-Seq and RNA-Seq technology to survey the transcript structures. We generated 14,013,757 sequence tags by H3K4me3 ChIP-Seq and obtained 17,322,358 paired end reads for mRNA, and 10,698,419 short reads for sRNA from the macaque brain. By integrating these data with genomic sequence features and extending and improving a state-of-the-art TSS prediction algorithm, we ab initio predicted and verified 17,933 of previously electronically annotated TSSs at 500-bp resolution. We also predicted approximately 10,000 novel TSSs. These provide an important rich resource for close examination of the species-specific transcript structures and transcription regulations in the Rhesus macaque genome. Our approach exemplifies a relatively inexpensive way to generate a reasonably reliable TSS map for a large genome. It may serve as a guiding example for similar genome annotation efforts targeted at other model organisms.
    Nucleic Acids Research 10/2010; 39(4):1408-18. DOI:10.1093/nar/gkq956
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    ABSTRACT: Tianjin Bin Hai New Area (BHNA) is the third largest economic zone in China. This is an older industrial area that has been developing rapidly but with many historic sources of contamination. The concentrations of 16 individual polycyclic aromatic hydrocarbons (PAHs) listed for priority control by the US EPA were quantified in 105 surface soil samples by the use of gas chromatography mass spectrometry (GC/MS). Multivariate statistical methods were used to characterise the mixtures and make comparisons in the patterns among areas. Concentrations varied among areas, ranging from moderate to relatively high. The coefficients of determination between concentrations of individual PAHs and total concentration of PAH (∑ PAH) increased with the number of rings. The first three factors from the principal component analysis (PCA) explained 81.6% of the total variation, while ten higher molecular weight (HMW) PAHs’ loadings for factor 1 accounted for 51.0%. Based on the patterns of relative loading proportions of individual PAHs calculated by PCA, the primary sources were coal combustion, traffic emissions including gasoline, diesel oil, and the burning of natural gas. The spatial distribution of concentrations of fluorene had a poor spatial autocorrelation in the study area. The sites with the greatest potential risk due to exposure to PAHs were mainly distributed around the chemical industry parks.
    Chemistry and Ecology 02/2010; 26(1):35-48. DOI:10.1080/02757540903468094
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    ABSTRACT: We introduced here a compact 500 GHz SIS (superconductor-insulator-superconductor) heterodyne receiver system developed for POST (portable submillimeter telescope). The system is proved to have a small volume to be integrated with the small Dewar, which is cooled by a compact close-cycled 100 mW@ 4 K cryo-cooler. Test results shows that a good LO coupling and low signal loss is achieved with a less than 250 K overall receiver noise at 500 GHz waveband.
    Microwave Conference, 2009. APMC 2009. Asia Pacific; 01/2010

Publication Stats

262 Citations
84.83 Total Impact Points

Institutions

  • 2006–2015
    • Chinese Academy of Sciences
      • • State Key Laboratory of Urban and Regional Ecology
      • • Center for Molecular Systems Biology
      • • Graduate School
      • • Purple Mountain Observatory
      Peping, Beijing, China
  • 2009–2014
    • National Space Science
      Peping, Beijing, China
  • 2008
    • Dalian Maritime University
      Lü-ta-shih, Liaoning, China
    • Chemical Abstracts Service (A division of the American Chemical Society)
      China, Maine, United States