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ABSTRACT: Spaceflight-induced microgravity appears to be a risk factor for the development of urinary calculi, resulting in urolithiasis during and after spaceflight. Calcifying nanoparticles, or nanobacteria, multiply more rapidly in simulated microgravity and create external shells of calcium phosphate. The question arises whether calcifying nanoparticles are nidi for calculi and contribute to the development of clinically significant urolithiasis in those who are predisposed to the development of urinary calculi because of intrinsic or extrinsic factors. This case report describes a calculus recovered after flight from an astronaut that, on morphologic and immunochemical analysis (including specific monoclonal antibody staining), demonstrated characteristics of calcifying nanoparticles.
Urology 09/2008; 73(1):210.e11-3. · 2.43 Impact Factor
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ABSTRACT: The etiology of nonbacterial prostatitis and chronic pelvic pain syndrome remain elusive and therefore therapy nonspecific,
mainly targeting symptom reduction. If an etiology could be identified, then more specific therapy, targeting the source,
could be developed. Acute and chronic prostatitis and other chronic prostate conditions are often associated with evidence
of inflammation, either acute, chronic, or both, as well as development of other histologic findings, such as corpora amylacea,
which contain calcium phosphate, aka apatite. There have been a number of chronic conditions/diseases that were originally
classified as idiopathic or noninfectious, such as peptic ulcer disease, which were later discovered to be infectious in origin.
Could such be the case for nonbacterial prostatitis and chronic pelvic pain syndrome (CPPS) Calcifying nanoparticles (CNP),
aka nanobacteria (NB) are small (50–200 nm), self-replicating entities that can be found in animal and human serum, urine,
and tissue and produce an outer shell of apatite. What is the likelihood that the CNPs are associated with the development
of inflammatory conditions of the prostate and possibly in the development of chronic prostatitis and chronic pelvic pain
syndrome? This chapter will examine the controversy surrounding NB/CNP, as well as the evidence for CNP association with human
genitourinary diseases especially that of the prostate.
KeywordsNanobacteria–nanoparticles–calcification–prostatitis–calcifying nanoparticles inflammation
06/2008: pages 221-243;
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The Lancet 07/2007; 369(9579):2078. · 38.28 Impact Factor
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Journal of Investigative Medicine 12/2006; 54(7):385-94. · 1.96 Impact Factor
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Yu Wen,
Yong-guo Li,
Zhu-lin Yang,
Xue-jun Wang,
Hong Wei,
Wei Liu,
Xiong-ying Miao,
Qun-wei Wang,
Sheng-fu Huang,
Jing Yang,
E Olavi Kajander, Neva Ciftcioglu
Chinese medical journal 04/2005; 118(5):421-4. · 0.86 Impact Factor
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ABSTRACT: The purpose of this preliminary study is to evaluate the effect of various wavelengths of light on nanobacteria (NB).
NB and mitochondria use light for biological processes. NB have been described as multifunctional primordial nanovesicles with the potential to utilize solar energy for replication. NB produce slime, a process common to living bacteria. Slime release is an evolutionary important stress-dependent phenomenon increasing the survival chance of individual bacteria in a colony. In the cardiovascular system, stress-induced bacterial colony formation may lead to a deposition of plaque.
Cultured NB were irradiated with NASA-LEDs at different wavelengths of light: 670, 728 and 880 nm. Light intensities were about 500k Wm(-2), and energy density was 1 x 10(4) J m(-2).
Monochromatic light clearly affected replication of NB. Maximum replication was achieved at 670 nm.
The results indicate that suitable wavelengths of light could be instrumental in elevating the vitality level of NB, preventing the production of NB-mediated slime, and simultaneously increasing the vitality level of mitochondria. The finding could stimulate the design of cooperative therapy concepts that could reduce death caused by myocardial infarcts.
Journal of Clinical Laser Medicine & Surgery 09/2003; 21(4):231-5.
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ABSTRACT: Kidney stone formation is a multifactorial disease in which the defence mechanisms and risk factors are imbalanced in favour of stone formation. We have proposed a novel infectious agent, mineral forming nanobacteria (NB), to be active nidi that attach to, invade and damage the urinary epithelium of collecting ducts and papilla forming the calcium phosphate center(s) found in most kidney stones. Stone formation may proceed in urine supersaturated with calcium phosphate, calcium oxalate and uric acid/urate under the influence of crystallization promoters and inhibitors. Our hypothesis underlines the role of active nidi: even supersaturated urine requires nidi for crystallization to appear.
Urological Research 07/2003; 31(2):47-54. · 1.23 Impact Factor
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Yu Wen,
Yong-guo Li,
Zhu-lin Yang,
Xue-jun Wang,
Hong Wei,
Wei Liu,
Ai-ling Tan,
Xiong-ying Miao,
Qun-wei Wang,
Sheng-fu Huang,
E Olavi Kajander, Neva Ciftcioglu
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ABSTRACT: To find the distribution of nanobacteria in the serum, bile and gallbladder mucosa of cholecystolithiasis patients.
The infection rate of nanobacteria was identified by ELISA in the serum samples from 338 healthy people and 76 patients with cholecystolithiasis (chi(2) = 0.89, P > 0.05). Nanobacteria were cultured from the bile samples in 57 patients with cholecystolithiasis and 18 non-cholelithiasis patients and identified by immunohistochemical staining and TEM (chi(2) = 29.80, P < 0.05). Forty samples of gallbladder mucosa randomly selected from the 57 cholecystolithiasis patients were identified by immunohistochemical staining and compared with the corresponding bile samples.
The infection rate of nanobacteria was 8.0% and 31.6% for the serum samples of the healthy people and cholecystolithiasis patients, respectively. The positive rate of nanobacteria in the bile samples was 61.3% and there was no significant difference in the bile of the cholecystolithiasis patients and the control group (61.4% vs. 61.1%). Fourteen positive patients had infection of nanobacteria in the gallbladder mucosa, submucosa, and calcific field.
The infection rate of nanobacteria was 8% in the serum samples from the healthy people. There are nanobacteria in the serum, bile, and gallbladder mucosa. The infection of the nanobacteria may result in calcification and fibrosis of the gallbladder.
Zhonghua wai ke za zhi [Chinese journal of surgery] 05/2003; 41(4):267-70.
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Andrei P. Sommer,,
David S. McKay,, Neva Ciftcioglu,,
Uri Oron,,
Adam R. Mester,,
E. Olavi Kajander
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ABSTRACT: Life on Earth and Mars could have started with self-assembled nanovesicles similar to the present nanobacteria (NB). To resist extreme environmental stress situations and periods of nutritional deprivation, nanovesicles would have had a chemical composition protected by a closed mineralized compartment, facilitating their development in a primordial soup, or other early wet environment. Their survivability would have been enhanced if they had mechanisms for metabolic communication, and an ability to collect primordially available energy forms. Here, we establish an irreducible model system for life formation starting with NB. Keywords: nanobacteria • nannobacteria • nanovesicles • extremophiles • origin of life models
03/2003;
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ABSTRACT: Endotoxin and nanobacteria in polycystic kidney disease.Background Microbes have been suspected as provocateurs of polycystic kidney disease (PKD), but attempts to isolate viable organisms have failed. Bacterial endotoxin is the most often reported microbial product found in PKD fluids. We assessed potential microbial origins of endotoxin in cyst fluids from 13 PKD patients and urines of PKD and control individuals.
Kidney International 05/2000; 57(6):2360-2374. · 6.61 Impact Factor
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ABSTRACT: The mechanisms of dental pulp stone formation are still largely unknown. Pulp stones are mainly composed of carbonate apatite. Only few experimental reports have elucidated the potential of some selected bacteria to produce apatite under in vitro conditions using special calcification media. The tested stone forming bacteria were, in fact, often better known for their cariogenic potential. Our preliminary work with 18 dental pulp stones from Turkey, selected only by severity of the stone formation, indicated the presence of nanobacterial antigens in the demineralized stones. Furthermore, high incidence of kidney stones and gall stones in the patient group and in their parents was found. This raises the implication that nanobacteria may enter the body also via oral route, in addition to the parenteral and transplacental routes. The role of nanobacteria in dental pulp stone formation was further studied by following nanobacterial colonization and mineral formation on human tooth in vitro. Two molar teeth, one having pulp stone and one without, were vertically cut into two pieces, sterilized by autoclaving and incubated with or without nanobacteria in DMEM. Electron microscopic observations indicate that nanobacteria can cause apatite stone formation on tooth surface. The sever from of dental pulp stone formation might be associated with nanobacteria. This form of dental disease results in loss of teeth due to osteolytic processes. This addresses the necessity for a study on unconventional mineral-forming bacteria as a cause for human diseases.© (1998) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
07/1998;
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ABSTRACT: Nanobacteria are the first mineral forming bacteria isolated from blood and blood products. They are coccoid cell-walled organisms with a size of 0.08 - 0.5 micrometers in EM, occure in clusters, produce a biofilm containing carbonate or hydroxyl apatite, and are highly resistant to heat, gamma-irradiation and antibiotics. Their growth rate is about one hundredth that of ordinary bacteria and they divide via several mechanisms. Taq polymerase was able to use their nontraditional nucleic acid as a template. 16S rRNA gene sequence results positioned them into the alpha-2 subgroup of Proteobacteria. Nanobacteria are smallest cell-walled bacteria since they can pass through 0.07 micrometers pores. In low-serum cultures, they form even smaller elementary particles or tubular units. How can blood be infected with such slow growing, heat and radio-resistant bacteria? The answer may lie in their phylogeny: alpha-2 subgroup has organisms from soil exposed to radiation and heat, that can penetrate into eukaryotic cells. Nanobacteria grow so slowly that they require a niche `cleaned' with heat, radiation or immunodefence. For survival they cloak themselves in apatite, a normal constituent of mammalian body. This may link nanobacteria to nannobacteria discovered from sedimentary rocks by Dr. Folk. Both have similar size, size variation, clustering and mineral deposits. They may resemble the probable ancient bacterial fossils in the Martian meteorite ALH84001.© (1997) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
07/1997;
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ABSTRACT: Life on Earth and Mars could have started with self-assembled nanovesicles similar to the present nanobacteria (NB). To resist extreme environmental stress situations and periods of nutritional deprivation, nanovesicles would have had a chemical composition protected by a closed mineralized compartment, facilitating their development in a primordial soup, or other early wet environment. Their survivability would have been enhanced if they had mechanisms for metabolic communication, and an ability to collect primordially available energy forms. Here, we establish an irreducible model system for life formation starting with NB.
Journal of Proteome Research 2(4):441-3. · 5.11 Impact Factor
