Andrew D Clouston

University of Queensland, Brisbane, Queensland, Australia

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Publications (174)1083.26 Total impact

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    ABSTRACT: Idiopathic Pneumonia Syndrome (IPS) is a relatively common, frequently fatal clinical entity characterized by non-infectious acute lung inflammation following allogeneic stem cell transplantation (SCT), the mechanisms of which are unclear. Here we demonstrate that immune suppression with cyclosporin after SCT limits Th1 differentiation and IFNγ secretion by donor T cells which is critical for inhibiting IL-6 generation from lung parenchyma during an alloimmune response. Thereafter, local IL-6 secretion induces donor alloantigen-specific Th17 cells to preferentially expand within the lung and blockade of IL-17A or transplantation of grafts lacking the IL-17 receptor prevents disease. Studies using IL-6(-/-) recipients or IL-6 blockade demonstrate that IL-6 is the critical driver of donor Th17 differentiation within the lung. Importantly, IL-6 is also dysregulated in patients undergoing clinical SCT and was present at very high levels in the plasma of patients with IPS compared to SCT recipients without complications. Furthermore, at the time of diagnosis, plasma IL-6 levels were higher in a subset of IPS patients who were non-responsive to steroids and anti-TNF therapy. In sum, pulmonary-derived IL-6 promotes IPS via the induction of Th17 differentiation and strategies that target these cytokines represent logical therapeutic approaches for IPS. Copyright © 2015 American Society of Hematology.
    Blood 02/2015; · 9.78 Impact Factor
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    ABSTRACT: Hepatocyte clone size was measured in liver samples of 21 patients in various stages of chronic hepatitis B virus (HBV) infection and from 21 to 76 years of age. Hepatocyte clones containing unique virus–cell DNA junctions formed by the integration of HBV DNA were detected using inverse nested PCR. The maximum hepatocyte clone size tended to increase with age, although there was considerable patient-to-patient variation in each age group. There was an upward trend in maximum clone size with increasing fibrosis, inflammatory activity and with seroconversion from HBV e-antigen (HBeAg)-positive to HBeAg-negative, but these differences did not reach statistical significance. Maximum hepatocyte clone size did not differ between patients with and without a coexisting hepatocellular carcinoma. Thus, large hepatocyte clones containing integrated HBV DNA were detected during all stages of chronic HBV infection. Using laser microdissection, no significant difference in clone size was observed between foci of HBV surface antigen (HBsAg)-positive and HBsAg-negative hepatocytes, suggesting that expression of HBsAg is not a significant factor in clonal expansion. Laser microdissection also revealed that hepatocytes with normal-appearing histology make up a major fraction of the cells undergoing clonal expansion. Thus, preneoplasia does not appear to be a factor in the clonal expansion detected in our assays. Computer simulations suggest that the large hepatocyte clones are not produced by random hepatocyte turnover but have an as-yet-unknown selective advantage that drives increased clonal expansion in the HBV-infected liver.
    Journal of Viral Hepatitis 01/2015; · 3.08 Impact Factor
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    ABSTRACT: To develop a model of stress-induced senescence to study the hepatocyte senescence associated secretory phenotype (SASP). Hydrogen peroxide treatment was used to induce senescence in the human HepG2 hepatocyte cell line. Senescence was confirmed by cytochemical staining for a panel of markers including Ki67, p21, heterochromatin protein 1β, and senescence-associated-β-galactosidase activity. Senescent hepatocytes were characterised by gene expression arrays and quantitative polymerase chain reaction (qPCR), and conditioned media was used in proteomic analyses, a human chemokine protein array, and cell migration assays to characterise the composition and function of the hepatocyte SASP. Senescent hepatocytes induced classical markers of senescence (p21, heterochromatin protein 1β, and senescence-associated-β-galactosidase activity); and downregulated the proliferation marker, Ki67. Hepatocyte senescence induced a 4.6-fold increase in total secreted protein (P = 0.06) without major alterations in the protein profile. Senescence-induced genes were identified by microarray (Benjamini Hochberg-corrected P < 0.05); and, consistent with the increase in secreted protein, gene ontology analysis revealed a significant enrichment of secreted proteins among inducible genes. The hepatocyte SASP included characteristic factors such as interleukin (IL)-8 and IL-6, as well as novel components such as SAA4, IL-32 and Fibrinogen, which were validated by qPCR and/or chemokine protein array. Senescent hepatocyte-conditioned medium elicited migration of inflammatory (granulocyte-macrophage colony stimulating factor, GM-CSF-derived), but not non-inflammatory (CSF-1-derived) human macrophages (P = 0.022), which could contribute to a pro-inflammatory microenvironment in vivo, or facilitate the clearance of senescent cells. Our novel model of hepatocyte senescence provides insights into mechanisms by which senescent hepatocytes may promote chronic liver disease pathogenesis.
    World journal of gastroenterology : WJG. 12/2014; 20(47):17851-62.
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    ABSTRACT: Background and AimsThere is increasing need to identify individuals with advanced liver fibrosis, who are at risk of complications such as hepatocellular carcinoma. The commercially available Enhanced Liver Fibrosis (ELF) test provides a non-invasive assessment of fibrosis severity. This study was designed to determine the diagnostic accuracy of the manufacturer's cut-off value (≥9.8) in identifying advanced fibrosis.Methods The relationship between ELF score and fibrosis was examined using serum collected at time of liver biopsy for investigation of liver disease, particularly viral hepatitis. Fibrosis was staged using a modified METAVIR score. If available, liver tissue was recut and stained with Sirius red to determine collagen proportional area and subsinusoidal fibrosis.ResultsELF score ≥9.8 had a sensitivity of 74.4% and specificity 92.4% for detecting advanced fibrosis. In the whole cohort (n=329), ELF score was more likely to incorrectly classify individuals if age was ≥45 years and METAVIR inflammatory grade was 2 or 3 (adjusted OR 3.71 and 2.62 respectively). In contrast, ELF score was less likely to misclassify individuals in the presence of steatosis (OR 0.37). Neither subsinusoidal fibrosis nor collagen proportional area explained the discordance in ELF score for patients with or without advanced fibrosis.Conclusion Although ELF score ≥9.8 reliably identifies advanced fibrosis in patients with chronic liver disease, both age and inflammatory activity need to be considered when interpreting the result. Importantly, ELF score performed well in the presence of steatosis and could thus be helpful in the assessment of fatty liver disease.This article is protected by copyright. All rights reserved.
    Liver international: official journal of the International Association for the Study of the Liver 12/2014; · 4.41 Impact Factor
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    ABSTRACT: The traditional serrated adenoma is the least common colorectal serrated polyp. The clinicopathological features and molecular drivers of these polyps require further investigation. We have prospectively collected a cohort of 200 ordinary and advanced traditional serrated adenomas and performed BRAF and KRAS mutational profiling, CpG island methylator phenotype analysis, and immunohistochemistry for a panel of 7 antibodies (MLH1, β-catenin, p53, p16, Ki67, CK7, and CK20) on all cases. The mean age of the patients was 64 years and 50% were female. Of the polyps, 71% were distal. Advanced histology (overt dysplasia or carcinoma) was present in 19% of cases. BRAF mutation was present in 67% and KRAS mutation in 22%. BRAF mutant traditional serrated adenomas were more frequently proximal (39% versus 2%; P≤0.0001), were exclusively associated with a precursor polyp (57% versus 0%; P≤0.0001), and were more frequently CpG island methylator phenotype high (60% versus 16%; P≤0.0001) than KRAS mutant traditional serrated adenomas. Advanced traditional serrated adenomas retained MLH1 expression in 97%, showed strong p53 staining in 55%, and nuclear β-catenin staining in 40%. P16 staining was lost in the advanced areas of 55% of BRAF mutant traditional serrated adenomas compared with 10% of the advanced areas of KRAS mutant or BRAF/KRAS wild-type traditional serrated adenomas. BRAF and KRAS mutant traditional serrated adenomas are morphologically related but biologically disparate polyps with distinctive clinicopathological and molecular features. The overwhelming majority of traditional serrated adenomas retain mismatch repair enzyme function indicating a microsatellite-stable phenotype. Malignant progression occurs via TP53 mutation and Wnt pathway activation regardless of mutation status. However, CDKN2A (encoding the p16 protein) is silenced nearly exclusively in the advanced areas of the BRAF mutant traditional serrated adenomas. Thus, the BRAF mutant traditional serrated adenoma represents an important precursor of the aggressive BRAF mutant, microsatellite-stable subtype of colorectal carcinoma.Modern Pathology advance online publication, 12 September 2014; doi:10.1038/modpathol.2014.122.
    Modern Pathology 09/2014; · 6.36 Impact Factor
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    ABSTRACT: Background: Fibrosis in liver with hepatitis C virus (HCV) recurrence post-liver transplantation (LT) can be rapidly progressive and the mechanisms underlying this process are poorly understood. In liver with HCV infection in the non-LT setting, there is a significant relationship between the development of structures known as the ductular reaction (DR), hepatic progenitor cells (HPCs) and fibrosis. In this study, we have characterized the DR, HPCs and fibrosis associated with HCV recurrence post-LT.Methods: Immunohistochemistry and confocal microscopy were used to characterize the DR, HPC and fibrosis in liver biopsy specimens. Key findings were confirmed in a separate independent cohort.Results: The initial characterization cohort had 194 biopsy samples from 105 individuals with HCV recurrence post-LT. The immunophenotype, morphology and location of the DR was consistent with an HPC origin. The DR correlated with intrahepatic fibrosis (rs=0.529, p<0.001) and numbers of activated hepatic stellate cells (HSCs, rs=0.446, p<0.001). There was an early occurrence of hepatocyte replicative arrest and increased hepatocyte proliferation that correlated with the DR (rs=0.295, p<0.001). Replicative arrest preceded hepatocyte proliferation in early stage injury. Hepatocyte proliferation decreased with advanced fibrosis, in contrast the extent of the DR and numbers of activated HSCs continued to increase. In the second cohort of 37 individuals, the DR and numbers of HPCs similarly correlated with fibrosis and inflammation post-LT.Conclusions: This is the first characterization of the DR in HCV-associated liver injury post-LT. There was a significant correlation between the DR and the development of progressive fibrosis in HCV recurrence. These results suggest a pivotal role for both the DR and HPC responses in the aggressive fibrosis seen with HCV recurrence post-LT. Liver Transpl , 2014. © 2014 AASLD.
    Liver Transplantation 09/2014; · 3.79 Impact Factor
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    ABSTRACT: Celiac disease (CeD) is a common gluten-sensitive autoimmune enteropathy. A gluten-free diet is an effective treatment, but compliance is demanding; hence, new treatment strategies for CeD are required.
    Journal of Allergy and Clinical Immunology 08/2014; 135(2). · 11.25 Impact Factor
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    ABSTRACT: Chronic GVHD (cGVHD) is the major cause of late, nonrelapse death following stem cell transplantation and characteristically develops in organs such as skin and lung. Here, we used multiple murine models of cGVHD to investigate the contribution of macrophage populations in the development of cGVHD. Using an established IL-17-dependent sclerodermatous cGVHD model, we confirmed that macrophages infiltrating the skin are derived from donor bone marrow (F4/80+CSF-1R+CD206+iNOS-). Cutaneous cGVHD developed in a CSF-1/CSF-1R-dependent manner, as treatment of recipients after transplantation with CSF-1 exacerbated macrophage infiltration and cutaneous pathology. Additionally, recipients of grafts from Csf1r-/- mice had substantially less macrophage infiltration and cutaneous pathology as compared with those receiving wild-type grafts. Neither CCL2/CCR2 nor GM-CSF/GM-CSFR signaling pathways were required for macrophage infiltration or development of cGVHD. In a different cGVHD model, in which bronchiolitis obliterans is a prominent manifestation, F4/80+ macrophage infiltration was similarly noted in the lungs of recipients after transplantation, and lung cGVHD was also IL-17 and CSF-1/CSF-1R dependent. Importantly, depletion of macrophages using an anti-CSF-1R mAb markedly reduced cutaneous and pulmonary cGVHD. Taken together, these data indicate that donor macrophages mediate the development of cGVHD and suggest that targeting CSF-1 signaling after transplantation may prevent and treat cGVHD.
    Journal of Clinical Investigation 08/2014; · 13.77 Impact Factor
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    ABSTRACT: Background and AimDevelopment of effective antifibrotic treatments which can be translated to clinical practice is an important challenge in contemporary hepatology. A recent report on β-thalassemia patients demonstrated that deferasirox treatment reversed or stabilized liver fibrosis independent of its iron chelating properties. In this study we investigated deferasirox in cell and animal models to better understand its potential antifibrotic effects.Methods The LX-2 stellate cell line was treated with 5μM or 50μM deferasirox (Exjade) for up to 120hr. Three week old multidrug resistance 2 null (Mdr2-/-) mice received oral deferasirox or vehicle for 4 weeks (30mg/kg/day). Cells and liver tissue were collected for assessment of fibrosis and fibrogenic gene expression.ResultsIn LX-2 cells treated with 50μM deferasirox for 12 hours α1(I)procollagen expression was decreased by 25%, with maximal reductions (10-fold) seen following 24-120 hours of treatment. Similarly, α-smooth muscle actin (αSMA) expression was significantly lower. Alterations in matrix remodelling genes, specifically decreased expression of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2, were observed. There was no significant difference in hepatic hydroxyproline content in Mdr2-/- mice following deferasirox administration (vehicle: 395±27μg/g vs. deferasirox: 421±33μg/g). Similarly, no changes in the expression of fibrogenic genes were observed.Conclusions Despite reductions in α1(I)procollagen and αSMA expression and alterations in matrix degradation genes in LX-2 cells, deferasirox did not exhibit antifibrotic activity in Mdr2-/- mice. Given the positive outcomes seen in human trials, it may be appropriate to study deferasirox in other animal models of fibrosis and/or for a longer duration of therapy.
    Journal of Gastroenterology and Hepatology 08/2014; · 3.33 Impact Factor
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    ABSTRACT: Background: Non-alcoholic steatohepatitis (NASH) is increasing in prevalence, yet the consequences for liver function are unknown. We studied ureagenesis, an essential metabolic liver function of importance for whole-body nitrogen homeostasis, in a rodent model of diet induced NASH. Methods: Rats were fed a high-fat, high-cholesterol diet for 4 and 16 weeks, resulting in early and advanced experimental NASH, respectively. We examined the urea cycle enzyme mRNAs in liver tissue, the hepatocyte urea cycle enzyme proteins and the in vivo Capacity of Urea-Nitrogen Synthesis (CUNS). Results: Early NASH decreased all the urea cycle mRNAs to an average of 60% and the ornithine transcarbamylase protein to 10% while the CUNS remained unchanged. Advanced NASH further decreased the carbamoyl phosphate synthetase protein to 63%, and in addition decreased the CUNS by 20% (from 5.65 ± 0.23 to 4.58 ± 0.30 μmol x (min x 100 g) -1; P = 0.01). Conclusion: Early NASH compromised the genes and enzyme proteins involved in ureagenesis, while advanced NASH resulted in a functional reduction in the capacity for ureagenesis. The pattern of urea cycle perturbations suggests a prevailing mitochondrial impairment by NASH. The decrease in CUNS has consequences for the ability of the body to adjust to changes in the requirements for nitrogen homeostasis e.g. at stressfull events. NASH, thus, in terms of metabolic consequences is not an innocuous lesion and the manifestations of the damage seem to be a continuum with increasing disease severity.
    AJP Gastrointestinal and Liver Physiology 06/2014; · 3.74 Impact Factor
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    ABSTRACT: Patients with non-alcoholic steatohepatitis (NASH) have increased mortality, including from infections. We, therefore, tested in a rodent model of steatohepatitis whether the hepatic acute phase response is intact. Steatohepatitis was induced in rats by feeding a high-fat, high-cholesterol diet for 4 (early) and 16 weeks (advanced NASH). 2 hours after low-dose LPS (0.5 mg/kg i.p.) we measured the serum concentrations of tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6). We also measured liver mRNA's and the serum concentrations of acute phase proteins 24 hours after LPS. NASH in itself increased the liver mRNA levels of TNF-α and IL-6 and also the liver mRNA and serum levels of the acute phase proteins. The exposure to LPS increased serum TNF-α in both early and advanced NASH and more so than in the control rats. However, the increases in acute phase protein genes in liver tissue and proteins in the blood were lower than in the control rats. In rats with early or advanced experimental NASH, LPS despite an increased interleukin release resulted in a blunted acute phase protein response. This tachyphylaxis may be part of the mechanism for the increased infection susceptibility of patients with NASH. We speculate that the steatosis-related interleukin release desensitizes the signalling pathway leading to acute phase protein synthesis. This article is protected by copyright. All rights reserved.
    Liver international: official journal of the International Association for the Study of the Liver 03/2014; · 4.41 Impact Factor
  • Human pathology 03/2014; 45(3):658–660. · 2.81 Impact Factor
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    ABSTRACT: Mammalian target of rapamycin and angiotensin converting enzyme inhibition has been shown to have antifibrotic activity in models of liver fibrosis. The aim of our study was to determine the efficacy of rapamycin, everolimus, irbesartan and captopril, alone and in combination, as antifibrotic agents in the Mdr2(-/-) model of cholestasis both in early injury and established disease. Mdr2(-/-) mice were treated for 4 weeks with vehicle, rapamycin (1 mg/kg) or everolimus (5 mg/kg) every second day or with captopril (30mg/kg/day), irbesartan (10 mg/kg/day) or vehicle. Further groups of three week old Mdr2(-/-) mice were treated with rapamycin and irbesartan in combination (1 mg/kg/day and 10 mg/kg/day) or with rapamycin (2 mg/kg/day) for 4 weeks. Liver injury and fibrosis were compared between treated and untreated animals. There were no significant improvements in liver injury, histology, hepatic hydroxyproline or profibrogenic gene expression following treatment with rapamycin, everolimus, captopril or irbesartan at any time point studied. Likewise, there were no improvements in liver histology or profibrogenic gene expression following combination therapy or high-dose rapamycin treatment. The antifibrotic effects of rapamycin, everolimus, captopril and irbesartan seen in other models of fibrosis were not replicated in the Mdr2(-/-) model in this study. This highlights the clear need to test specific antifibrotic agents in a number of different animal models. We believe this animal model is ideal to study usefulness of antifibrotic agents in cholestatic liver disease because of the similarity in genetics and hepatic histopathology to human cholestatic liver disease. This article is protected by copyright. All rights reserved.
    Liver international: official journal of the International Association for the Study of the Liver 02/2014; · 4.41 Impact Factor
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    ABSTRACT: Non-alcoholic fatty liver disease (NAFLD) is a rapidly growing global health problem. It can be separated histologically into two broad groups: steatosis, which usually follows a benign clinical course and non-alcoholic steatohepatitis (NASH) that typically has hepatocyte ballooning, necroinflammatory activity and can progress to fibrosis and cirrhosis. More recently the histological spectrum has expanded with the recognition of a paediatric pattern of NASH that has portal-based inflammation and fibrosis without ballooning. An overlap pattern is also described. There is increasing interest in the portal changes of NASH as these correlate with the progression of fibrosis. Disease-associated hepatocyte senescence appears to trigger an alternative regenerative pathway and the development of a periportal ductular reaction (DR), which in turn may have a role in progressive fibrogenesis. Portal inflammation, particularly in association with the DR, is an area of recent study.
    Current Hepatitis Reports 02/2014;
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    ABSTRACT: The sessile serrated adenoma (SSA) is a relatively recently described polyp that can present diagnostic difficulties for the practicing pathologist. The frequency of SSA diagnoses varies dramatically in the reported literature. In addition, the histologic interface between the microvesicular hyperplastic polyp (MVHP) and the SSA continues to be a diagnostic problem. The trend in recent years has been toward a lower threshold for SSA diagnosis. Herein, we have performed a cross-sectional study of 6340 colorectal polyps received at a high-volume community-based pathology practice over a 3-month period. After central review, with strict application of the diagnostic criteria outlined in the 2010 edition of the World Health Organization Classification of Tumours of the Digestive Tract, we found that SSAs represented 12.1% of all polyps. In addition, we developed novel diagnostic subcategories in an attempt to determine the most appropriate cutoff for the interface between the MVHP and the SSA. We found that serrated polyps (MVHPs or SSAs) with any SSA-like crypts had clinical features more in common with the SSA than the MVHP and that this diagnostic cutoff showed good reproducibility between pathologists. This supports the position of a recent consensus publication proposing that polyps with as few as 1 SSA-type crypt should be diagnosed as an SSA. Applying these criteria to our cohort yields an overall SSA rate of 14.7%. In summary, we believe that SSAs continue to be underdiagnosed in pathologic practice and that this may result in inadequate surveillance and thus contribute to interval colorectal carcinomas.
    The American journal of surgical pathology 02/2014; 38(2):158-66. · 4.59 Impact Factor
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    ABSTRACT: The normal liver has three major closely integrated compartments: hepatocytes, the biliary system and the vascular system. The first comprises 70% of the cells; the biliary system communicates throughout and outside the liver with immediately adjacent extra-hepatic organs and the last is, in many ways, the most complex of all three systems. The vascular system also communicates outside and throughout the liver. This article is protected by copyright. All rights reserved.
    Histopathology 01/2014; · 3.30 Impact Factor
  • Michael J Williams, Andrew D Clouston, Stuart J Forbes
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    ABSTRACT: Interactions between cells and their extracellular matrix have been shown to be crucial in a wide range of biological processes, including the proliferation and differentiation of stem cells. Ductular reactions containing both hepatic progenitor cells and extracellular matrix are seen in response to acute severe and chronic liver injury. Understanding the molecular mechanisms whereby cell-matrix interactions regulate liver regeneration may allow novel strategies to enhance this process. Both the ductular reaction in humans and hepatic progenitor cells in rodent models are closely associated with collagen and laminin, although there is still debate about cause and effect. Recent studies have demonstrated a requirement for matrix remodelling by matrix metalloproteinases for the proliferation of hepatic progenitor cells, and suggested defined roles for specific matrix components. Understanding the interactions between progenitor cells and matrix is critical for the development of novel regenerative and anti-fibrotic therapies.
    Gastroenterology 12/2013; · 12.82 Impact Factor
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    ABSTRACT: Although non-alcoholic fatty liver disease (NAFLD) is conventionally assessed histologically for lobular features of inflammation, the development of portal fibrosis appears to be associated with disease progression. We investigated the composition of the portal inflammatory infiltrate and its relationship to the ductular reaction (DR), a second portal phenomenon implicated in fibrogenesis. The portal inflammatory infiltrate may contribute directly to fibrogenesis as well as influence the fate of the DR hepatic progenitor cells (HPC), regulating the balance between liver repair and fibrosis. The presence of portal inflammation in NAFLD was strongly correlated with disease severity (fibrosis stage) and the DR. The portal infiltrate was characterised by immunostaining NAFLD liver biopsy sections (n=33) for broad leukocyte subset markers (CD68, CD3, CD8, CD4, CD20, neutrophil elastase) and selected inflammatory markers (Matrix Metalloproteinase-9, IL-17). Cells expressing all markers examined were identified throughout the liver lobules and in portal tracts, although portal tracts were more densely populated (P<0.01), and dominated by CD68+ macrophages and CD8+ lymphocytes, at all stages of disease. An increase in portal macrophages in NAFLD patients with steatosis alone (P<0.01) was the earliest change detected, even prior to elevated expression of the pro-inflammatory cytokines IL1B and TNF in patients with early NASH (P<0.05). Portal and peri-ductal accumulation of all other cell types examined occurred in progressed NASH (all P<0.05). Conclusion: Knowledge of the complex cellular composition of the portal inflammatory infiltrate and HPC/DR niche in NAFLD will shape future functional studies to elucidate the contribution of portal inflammation to HPC differentiation and NAFLD pathogenesis.
    Hepatology 11/2013; · 11.19 Impact Factor
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    ABSTRACT: Aims: Type 2 diabetes is a risk factor for the development and progression of non-alcoholic fatty liver disease (NAFLD). Lifestyle intervention is the principal treatment for NAFLD however the effects of exercise alone on the histological and metabolic severity of NAFLD are unclear. This study assessed the effects of 6 months exercise training and diet-induced weight loss on insulin resistance and liver histologyin overweight patients with NAFLD. Methods: 21 patients were randomised to circuit exercise (EX) training (60 min×3/week) or dietary induced weight loss (DIWL) (-500 kcal/d). Insulin sensitivity (euglycaemic-hyperinsulinemic clamp with tracer), adiposity (CT scan) and histology (liver biopsy) were assessed at 0 and 6 months. Results: Weight decreased by 9.7 ± 4.6% (-6.7 ± 6.3 kg p=0.02) with DIWL but was unchanged after EX. Both groups equivalently reduced visceral fat (DIWL -22 ± 24% p=0.06 and EX -18 ± 18% p<0.05) while only EX increased lean mass (+3% p<0.01). DIWL markedly reduced steatosis (73 ± 36% to 23 ± 32%, p<0.05) and NAFLD activity score NAS (median (range) 5 (1-7) to 1 (0-5), p<0.05). After EX, there was no change in steatosis or NAS. A decrease in steatosis was associated with weight loss (rs=0.82, p<0.0001). An improvement in fibrosis was associated with a decrease in steatosis (rs=0.64, p=0.02). Small improvements in fasting hepatic insulin resistance were similar in both groups while changes in muscle insulin resistance were not significant. Conclusions: Circuit exercise is safe and efficacious for improving cardiometabolic risk factors in patients with NAFLD, however this dose of circuit training, without concomitant weight loss, was insufficient for histological improvements in NAFLD. The pilot study outcomes should stimulate further development of different exercise protocols (type, frequency and intensity) to address disease-specific conditions in those with severe insulin resistance.
    Journal of Diabetes & Metabolism 10/2013; 4(8).
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    ABSTRACT: Endoplasmic reticulum (ER) stress is an important pathogenic mechanism for alcoholic (ALD) and nonalcoholic fatty liver disease (NAFLD). Iron overload is an important cofactor for liver injury in ALD and NAFLD, but its role in ER stress and associated stress signaling pathways is unclear. To investigate this, we developed a murine model of combined liver injury by co-feeding the mildly iron overloaded, the hemochromatosis gene-null (Hfe(-/)) mouse ad libitum with ethanol and a high-fat diet (HFD) for 8 weeks. This co-feeding led to profound steatohepatitis, significant fibrosis, and increased apoptosis in the Hfe(-/-) mice as compared with wild-type (WT) controls. Iron overload also led to induction of unfolded protein response (XBP1 splicing, activation of IRE-1α and PERK, as well as sequestration of GRP78) and ER stress (increased CHOP protein expression) following HFD and ethanol. This is associated with a muted autophagic response including reduced LC3-I expression and impaired conjugation to LC3-II, reduced beclin-1 protein, and failure of induction of autophagy-related proteins (Atg) 3, 5, 7, and 12. As a result of the impaired autophagy, levels of the sequestosome protein p62 were most elevated in the Hfe(-/-) group co-fed ethanol and HFD. Iron overload reduces the activation of adenosine monophosphate protein kinase associated with ethanol and HFD feeding. We conclude that iron toxicity may modulate hepatic stress signaling pathways by impairing adaptive cellular compensatory mechanisms in alcohol- and obesity-induced liver injury.Laboratory Investigation advance online publication, 14 October 2013; doi:10.1038/labinvest.2013.121.
    Laboratory Investigation 10/2013; · 3.96 Impact Factor

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5k Citations
1,083.26 Total Impact Points

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  • 1997–2015
    • University of Queensland
      • • Centre for Liver Disease Research
      • • Department of Medicine
      • • School of Medicine
      • • Department of Surgery
      Brisbane, Queensland, Australia
  • 2014
    • Translational Research Institute
      Sydney, New South Wales, Australia
  • 2004–2013
    • Queensland Institute of Medical Research
      • Bone Marrow Transplantation Laboratory
      Brisbane, Queensland, Australia
  • 1993–2013
    • Princess Alexandra Hospital (Queensland Health)
      • • Division of Medicine
      • • Division of Surgery
      Brisbane, Queensland, Australia
  • 2004–2012
    • University of Southern Queensland 
      Toowoomba, Queensland, Australia
  • 2009
    • Wesley Hospital
      Brisbane, Queensland, Australia
  • 2007
    • University of Melbourne
      Melbourne, Victoria, Australia
  • 2005
    • University of Pittsburgh
      • Department of Medicine
      Pittsburgh, Pennsylvania, United States
  • 2002
    • Sullivan Nicolaides Pathology
      Taringa, Queensland, Australia