Ailing Sun

Liaocheng Teachers University, Tungchangfu, Shandong Sheng, China

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Publications (40)101.95 Total impact

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    ABSTRACT: A preparative high speed counter-current chromatography (HSCCC) method for isolation and purification of flavonoid compounds from SCUTELLARIAE RADIX, the roots of Chinese medicinal plant Scutellaria baicalensis Georgi, was successfully established by using ionic liquids as the modifier of the two-phase solvent system. Baicalin and wogonoside were purified from the crude extract of SCUTELLARIAE RADIX by using ethyl acetate-water-[C4mim][PF6] (5:5:0.2, v/v) as two-phase solvent system. From 120 mg of the crude sample, 50.1 mg of baicalin and 45.6 mg of wogonoside were obtained in a one step separation. The purities of baicalin and wogonoside were 99.3% and 99.1%, respectively, as determined by HPLC area normalization method. The chemical structures of the isolated compounds were identified by1H-NMR and 13C-NMR.
    Journal of Liquid Chromatography &amp Related Technologies 01/2014; 37(16). · 0.57 Impact Factor
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    ABSTRACT: In this study, a simple and efficient preparative procedure was developed for preparation of seven flavonoids from the peel of Trichosanthes kirilowii Maxim. using polyamide resin followed by semi-preparative high performance liquid chromatography (SPHPLC). First, the ethyl acetate fraction from the peel of T. kirilowii Maxim. obtained “prefractionation” using polyamide resin, which yielded two subfractions. And then the two subfractions were isolated by SPHPLC with an isocratic elution of methanol–water. Finally, seven known flavonoids were purified from 35 g of ethyl acetate extract including quercetin-3-O-[α-l-rhamnose (1→2)-β-d-glucopyranosyl]-5-O-β-d-glucopyranoside (19 mg), quercetin-3-O-rutinoside (24 mg), apigenin-7-O-β-d-glucopyranoside (10 mg), diosmetin-7-O-β-d-glucopyranoside (45 mg), luteolin (21 mg), apigenin (15 mg), and diosmetin (56 mg). The purities of the compounds were determined by HPLC and the chemical structures were confirmed by UV and NMR analysis. In the present study, a simple, effective, and rapid procedure was established for preparative separation of multiple components from the peel of T. kirilowii Maxim. Furthermore, it was scalable and economical, so it was a promising basis for large-scale preparation of flavonoids from other plant extracts.
    Journal of Chromatography B. 01/2014; 965:150–157.
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    ABSTRACT: In this paper, a chromatographic method for isolation and purification of coumarin compounds from Cortex fraxinus was established by using Superose 12 as the separation media for the first time. The conditions for separation were optimized. Four kinds of coumarin compounds including aesuletin, aesculin, fraxetin and fraxin were obtained. The purity of these compounds were 98.5, 99.1, 97.9 and 97.3%, respectively, which were determined by HPLC area normalization method. The chemical structures of the separated compounds were identified according to (1)H and (13)C nuclear magnetic resonance data. The retention behavior of the separated coumarin compounds on Superose 12 was also discussed. The retention is based on a mixture of hydrogen bonding and hydrophobic interactions between the coumarin compounds and the residues of the cross-linking reagents used in the manufacturing process of Superose 12. The results of this paper indicate that Superose 12 is not only suitable for size-exclusion chromatography of proteins and other biological macromolecules but also for low-molecular-weight natural products.
    Journal of chromatographic science 10/2013; · 0.79 Impact Factor
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    ABSTRACT: A reversed phase high performance liquid chromatography (RP-HPLC) method for simultaneous determination of fangchinoline (FAN) and tetrandrine (TET) in Stephania tetrandra S. Moore was established by using 1-hexyl-3-methylimidazolium tetrafluoroborate as the mobile phase additives in this paper. Four types of 1-alkyl-3-methylimidazolium-based ionic liquids (ILs) were used as additives of the mobile phase to separate FAN and TET by RP-HPLC. The effects of the length of the alkyl group on the imidazolium ring and its counterion, the concentrations of IL and the pH of the mobile phase, which influenced the chromatographic behaviors of FAN and TET, were investigated in detail. The linearity, sensitivity, accuracy and repeatability of the proposed method were also investigated. The probable mechanism of the separation with ILs as the mobile phase additives was explored and discussed.
    Analytica chimica acta 03/2013; 767:148-54. · 4.31 Impact Factor
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    ABSTRACT: A chromatographic method for isolation and purification of soy isoflavones from soybean extracts was established by using 12% cross-linked agarose gel, Superose 12, as the separation media. 30% and 65% methanol in gradient elution mode was employed for separation. Soybean extracts were separated by the proposed method and six soy isoflavones, including glycitin, daidzin, genistin, glycitein, daidzein, and genistein, were obtained. The purity of these compounds was 97.8%, 98.5%, 99.4%, 99.0%, 98.8%, 99.2%, respectively, which was determined by HPLC area normalization method. The retention behavior of soy isoflavones on Superose 12 was also discussed. The retention is based on a mixture of hydrogen bonding and hydrophobic interactions between the hydroxyl groups of soy isoflavones and the residues of the cross-linking reagents used in the manufacturing process of Superose 12.
    Journal of Liquid Chromatography &amp Related Technologies 01/2013; 36(16). · 0.57 Impact Factor
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    ABSTRACT: High-speed counter-current chromatography (HSCCC) and evaporative light scattering detector (ELSD) were connected together by flow injection for the first time. A new method for isolation and purification of ginkgolide compounds from GINKGO BILOBA L. was developed. Two pairs of two-phase solvent system, n-hexane-ethyl acetate-methanol-water (4:5:1:5, v/v) and n-hexane-ethyl acetate-methanol-water (4:5:2:5, v/v), were used in HSCCC separation. The stationary phase was the upper phase of n-hexane-ethyl acetate-methanol-water (4:5:1:5, v/v). The mobile phase was the lower phase of n-hexane-ethyl acetate-methanol-water (4:5:1:5, v/v) and n-hexane-ethyl acetate-methanol-water (4:5:2:5, v/v) in gradient elution mode. GINKGO BILOBA L. powders were degreased by light petroleum and extracted with 70% ethanol. The extracts were purified with ethyl acetate extraction and acidic alumina oxide column separation, and 2.4 g of purified extracts were obtained from 500 g of GINKGO BILOBA L. 107 mg of ginkgolides A, 64 mg of ginkgolides B, 52 mg of ginkgolides C, and 83 mg of bilobalide were obtained in one-step HSCCC separation from 500 mg of the purified extracts. The purities of the obtained compounds were all above 98% as determined by HPLC-ELSD area normalization method and their chemical structures were identified by 1H-NMR and 13C-NMR.
    Journal of Liquid Chromatography &amp Related Technologies 01/2013; 36(16). · 0.57 Impact Factor
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    ABSTRACT: A chromatographic method for isolation and purification of active components from Polygonum cuspidatum Sieb. et Zucc was established by using Superose 12 as the separation media. 65% methanol was used as the mobile phase for separation. Four components including piceid, resveratrol, anthraglycoside B and emodin were obtained in one-step separation. The purity of these compounds was 98.3%, 99.3%, 98.7%, 99.1%, respectively, as determined by HPLC area normalization method. The chemical structures of these compounds were identified according to their H NMR and C NMR data.
    Journal of Liquid Chromatography &amp Related Technologies 01/2012; · 0.57 Impact Factor
  • Aifeng Li, Ailing Sun, Renmin Liu
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    ABSTRACT: High-speed counter-current chromatography was applied in the isolation and purification of three flavonoids from the Chinese medicinal plant Alpinia katsumadai Hayata. Two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water at volume ratios of 5:5:7:3 was used, which yielded 17.2 mg of alpinetin, 3.5 mg of pinocembrin, and 25.1 mg of cardamomin from 100 mg of the crude extract in one-step separation. The purities of them were 98.1%, 97.5% and 99.2% as determined by high performance liquid chromatography and their chemical structures were identified by H-NMR and C-NMR.
    Journal of Liquid Chromatography &amp Related Technologies 01/2012; · 0.57 Impact Factor
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    ABSTRACT: A preparative high-speed counter-current chromatography (HSCCC) method for separation and purification of biondnoid I and magnolin from the Chinese medicinal plant FLOS MAGNOLIAE was successfully established by using light petroleum-ethyl acetate-methanol-water (3:7:4:6, v/v) as the two-phase solvent system. From 500 mg of crude extracts of FLOS MAGNOLIAE 48 mg of biondnoid I with the purity of 99.3% and 135 mg of magnolin with the purity of 98.7% were separated in a one-step separation. The structures of biondnoid I and magnolin were identified by H-NMR.
    Journal of Liquid Chromatography &amp Related Technologies 01/2012; · 0.57 Impact Factor
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    ABSTRACT: A new high speed counter-current chromatography (HSCCC) method for preparative separation and purification of three phenolic acids from the dried buds of Lonicera japonica Thunb was developed by using gradient elution mode. Two pairs of two-phase solvent system composed of n-hexane-ethyl acetate-methanol-0.01 M hydrochloric acid at volume ratios of 1:5:1:5 and 1:5:2:5 were used in HSCCC separation. The upper phase of n-hexane-ethyl acetate-methanol-0.01 M hydrochloric acid (1:5:1:5, v/v) was used as the stationary phase. The lower phase of n-hexane-ethyl acetate-methanol-0.01 M hydrochloric acid (1:5:1:5, v/v) and (1:5:2:5, v/v) were used as the mobile phase in gradient elution mode. From 200 mg of the crude extract 51.6 mg of 3-caffeoylquinic acid, 7.5 mg of caffeic acid, and 42.4 mg of 3,5-O-dicaffeoylquinic acid were obtained in one-step HSCCC separation. The purities of the obtained compounds were all above 95% as determined by HPLC area normalization method, and their chemical structures were identified by H-NMR and C-NMR.
    Journal of Liquid Chromatography & Related Technologies - J LIQ CHROMATOGR RELAT TECHNO. 01/2012;
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    ABSTRACT: High-speed counter-current chromatography was applied in the isolation and purification of four isoflavonoids from the Chinese medicinal plant Belamcanda chinensis(L.) DC. Preparative high-speed counter-current chromatography method with two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water at volume ratio of 5:5:3:7 was successfully established, which yielded 6.2 mg of tectoridin, 8.5 mg of iridin, 22.6 mg of irigenin and 13.4 mg of irisflorentin from 150 mg of the crude extract in one-step separation. The purities were 98.1%, 98.9%, 99.0% and 99.2%, respectively, as determined by high performance liquid chromatography and their chemical structures were identified by H-NMR and C-NMR.
    Journal of Liquid Chromatography & Related Technologies - J LIQ CHROMATOGR RELAT TECHNO. 01/2012;
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    ABSTRACT: A method for extraction and preparative separation of tanshinones from Salvia miltiorrhiza Bunge was successfully established in this paper. Tanshinones from Salvia miltiorrhiza Bunge were extracted using ethyl acetate as the extractant under reflux. The extracts were then purified by high speed counter-current chromatography (HSCCC) with light petroleum-ethyl acetate-methanol-water (6:4:6.5:3.5, v/v) as the two phase solvent system. The upper phase was used as the stationary phase and the lower phase as the mobile phase. 8.2mg of dihydrotanshinone I, 5.8 mg of 1,2,15,16-tetrahydrotanshiquinone, 26.3mg of cryptotanshinone, 16.2mg of tanshinone I, 25.6 mg of neo-przewaquinone A, 68.8 mg of tanshinone IIA and 9.3mg of miltirone were obtained from 400mg of extracts from Salvia miltiorrhiza Bunge in one-step HSCCC separation, with the purity of 97. 6%, 95.1%, 99.0%, 99.1%, 93.2%, 99.3% and 98.7%, respectively, as determined by HPLC area normalization method. Their chemical structures were identified by ¹H NMR.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 07/2011; 879(21):1899-904. · 2.78 Impact Factor
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    ABSTRACT: A new high-speed counter-current chromatography method for semi-preparative separation and purification of alkaloids from embryo of the seed of Nelumbo nucifera Gaertn was developed by using pH-gradient elution mode. Diethyl ether was used as the stationary phase of the two-phase solvent system and Na(2)HPO(4)/NaH(2)PO(4) buffer solution with pH values of 7.5 and 7.2 in gradient mode as the mobile phase. Consequently, 33 mg of liensinine, 42 mg of isoliensinine, and 67 mg of neferine were obtained from 200 mg of crude extracts. The purities of them were all over 98% as determined by HPLC area normalization method, and the structures were identified by (1)H-NMR and (13)C-NMR.
    Journal of Separation Science 06/2010; 33(12):1746-51. · 2.59 Impact Factor
  • Renmin Liu, Lili Xu, Aifeng Li, Ailing Sun
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    ABSTRACT: A preparative high-speed counter-current chromatography method for isolation and purification of flavonoid compounds from Oroxylum indicum was successfully established by using ionic liquids as the modifier of the two-phase solvent system. Two flavonoid compounds including baicalein-7-O-diglucoside and baicalein-7-O-glucoside were purified from the crude extract of O. indicum by using ethyl acetate-water-[C(4)mim][PF(6)] (5:5:0.2, v/v) as two-phase solvent system. 36.4 mg of baicalein-7-O-diglucoside and 60.5 mg of baicalein-7-O-glucoside were obtained from 120 mg of the crude extract. Their purities were 98.7 and 99.1%, respectively, as determined by HPLC area normalization method. The chemical structures of the isolated compounds were identified by (1)H-NMR and (13)C-NMR.
    Journal of Separation Science 02/2010; 33(8):1058-63. · 2.59 Impact Factor
  • Lili Xu, Aifeng Li, Ailing Sun, Renmin Liu
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    ABSTRACT: A preparative high-speed countercurrent chromatography method for isolation and purification of neomangiferin and mangiferin from Rhizoma anemarrhenae was successfully established by using ionic liquids as the modifier of the two-phase solvent system. Neomangiferin and mangiferin were purified from the crude extract of R. anemarrhenae by using ethyl acetate-water-[C(4)mim][PF(6)] (5:5:0.2 v/v) as two-phase solvent system. In total, 22.5 mg of neomangiferin and 70.6 mg of mangiferin were obtained from 150 mg of the crude extract. The purities of neomangiferin and mangiferin were 97.2 and 98.1%, respectively, as determined by HPLC. The chemical structures of the isolated compounds were identified by (1)H-NMR and (13)C-NMR.
    Journal of Separation Science 11/2009; 33(1):31-6. · 2.59 Impact Factor
  • Renmin Liu, Sujuan Wu, Ailing Sun
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    ABSTRACT: A preparative high-speed counter-current chromatography (HSCCC) method for the isolation and purification of 1''-O-glucosylcimifugin (1), 4'-O-beta-d-glucosyl-5-O-methylvisamminol (2), cimifugin (3) and 3'-O-glucosylhamaudol (4) from the Chinese medicinal herb radix saposhnikoviae has been successfully developed. A sample of 300 mg of crude extract was separated using ethyl acetate:n-butanol:1% aqueous acetic acid (1:4:5, v/v) as the two-phase solvent system and yielded 102.4 mg of 1 and 81.6 mg of 2. During this separation 3 and 4 remained in the stationary phase, which was collected, evaporated to dryness and separated with another two-phase solvent system involving ethyl acetate:n-butanol:1% aqueous acetic acid (5:0.5:5, v/v) to yield 31.4 mg of 3 and 12.7 mg of 4. The purities of compounds 1-4 were 98.4, 98.7, 99.3 and 98.2%, respectively, as determined by HPLC. The chemical structures of these components were established by (1)H-NMR and (13)C-NMR.
    Phytochemical Analysis 06/2008; 19(3):206-11. · 2.48 Impact Factor
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    ABSTRACT: A chromatographic method for isolation and purification of chemical constituents from the well-known traditional Chinese drug Da-huang (roots of Rheum officinale Baill.) was established by using 12% cross-linked agarose gel, Superose 12, as the separation media. A two-step separation procedure is employed. Sixty five percent methanol was used as the eluent for separation of cinnamic acid, rhein, physcion and emodin form Da-huang crude extract. The fraction containing aloe-emodin and chrysophanol was then separated by using 55% methanol containing 0.5% acetic acid as the eluent. As a result, cinnamic acid and five kinds of hydroxyanthraquinones including rhein, aloe-emodin, chrysophanol, physcion and emodin were obtained. The retention behavior of hydroxyanthraquinones on Superose 12 was also studied. The retention of hydroxyanthraquinones on Superose 12 is based on a mixture of hydrogen bonding and hydrophobic interactions between the hydroxyl groups of the hydroxyanthraquinones and the residues of the cross-linking reagents used in the manufacturing process of Superose 12.
    Journal of Separation Science 03/2008; 31(2):283-7. · 2.59 Impact Factor
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    ABSTRACT: A chromatographic method using 12% cross-linked agarose gel Superose 12 as the separation medium was developed for isolation and purification of the chemical constituents from the pericarp of Sophora japonica L. The mobile phase used for the separation was 2% acetic acid and 7% acetic acid in gradient elution. As a result, eight compounds including four kinds of flavonoids and four kinds of isoflavonoids were obtained in a one-step separation. A straightforward explanation of the separation mechanism of flavonoids and isoflavonoids on Superose 12 is also given. The flavonoids and isoflavonoids are retained on Superose 12 by a combination of hydrogen bonding and hydrophobic interactions between the hydroxyl groups of aglycone and the residues of the cross-linking reagents used in the manufacture of Superose 12.
    Journal of Separation Science 09/2007; 30(12):1870-4. · 2.59 Impact Factor
  • Ailing Sun, Qinghua Sun, Renmin Liu
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    ABSTRACT: High-speed counter-current chromatography combined with macroporous resin column separation was applied to the isolation and purification of genistein-7,4'-di-O-beta-D-glucoside (I), genistein-7-O-beta-D-glucopyranoside-4'-O-[(alpha-L-rhamnopyransoyl)-(1-2)-beta-D-glucopyranoside] (II), kaempferol-3-O-beta-D-sophoroside(III), quercetin-3-O-beta-L-ramnopyranosyl-(1 - 6)-beta-D-glucopyranoside (IV), genistein-4'-beta-L-rhamnopyransoyl-(1 - 2)-alpha-D-glucopyranoside (V), and kaempferol-3-O-beta-L-ramnopyranosyl-(1 - 6)-beta-D-glucopyranoside (VI) from the Chinese medicinal herb Sophora japonica L. The crude extracts from the pericarps of Sophora japonica L. were pre-separated on a D-101 macroporous resin column and divided into two parts as sample 1 and sample 2. An 80-mg portion of sample 1 was separated by using n-butanol-acetic acid (1%) (5:5, v/v) as the two-phase solvent system and yielded 30.1 mg of compound I, 23.3 mg of compound II. A 120 mg portion of sample 2 was separated by using ethyl acetate-n-butanol-acetic acid (1%) (5:0.8:5, v/v) as the two-phase solvent system and yielded 5.5 mg of compound III, 31.7 mg of compound IV, 37.4 mg of compound V, and 6.2 mg of compound VI. The purities of compounds I, II, III, IV, V, and VI were 98.7, 98.2, 97.8, 98.5, 99.3, and 98.9%, respectively, as determined by HPLC. The chemical structures of these components were identified by 1H-NMR and 13C-NMR.
    Journal of Separation Science 06/2007; 30(7):1013-8. · 2.59 Impact Factor
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    ABSTRACT: A method for isolation and purification of flavonoid and isoflavonoid compounds in extracts of the pericarp of Sophora japonica L. was established by adsorption chromatography on the 12% cross-linked agarose gel Superose 12. The crude extracts were pre-separated to two parts, sample A and sample B, on a D-101 macroporous resin column by elution with 20% ethanol and 40% ethanol, respectively. Samples A and B were then separated by adsorption chromatography on Superose 12 with 40% methanol as the mobile phase. Eight compounds including four kinds of flavonoids and four kinds of isoflavonoids were obtained by the proposed method. The adsorption mechanisms of flavonoids and isoflavonoids on Superose 12 were also discussed.
    Journal of Chromatography A 02/2007; 1140(1-2):219-24. · 4.61 Impact Factor