T V Osipova

Publications (5)1.58 Total impact

• Article: Development of a biochip with an internal calibration curve for quantitating two forms of the prostate-specific antigen

  E. V. Konovalova, E. N. Savvateeva, E. I. Dementieva, M. A. Filippova, A. Yu. Turygin, T. V. Osipova, T. P. Ryabykh, A. Yu. Rubina, A. S. Zasedatelev
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  ABSTRACT: Three-dimensional gel-based biological microchips were developed for simultaneous quantitation of total (PSAtot) and free (PSAfree) forms of the prostate-specific antigen in human serum in the “one patient, one biochip” format. A method not demanding construction of calibration curves prior to the assay was applied to quantitation of PSAtot and PSAfree. In addition to gel elements with immobilized antibodies against PSAtot and PSAfree, the biochip contains elements with immobilized PSA at different concentrations, forming an internal calibration curve. Data are processed and interpreted with the special-purpose ImaGelAssay program. The sensitivity of the assay is 0.3 ng/ml for PSAtot and 0.2 ng/ml for PSAfree. The variation coefficient for measurements with one biochip series does not exceed 10%. The correlation coefficients between the estimates obtained for human sera by the biochip assay and by conventional ELISA were 0.988 for PSAtot and 0.987 for PSAfree.
  Molecular Biology 04/2012; 41(4):665-669. · 0.66 Impact Factor
• Article: Biological microchip for a simultaneous quantitative immunoassay of tumor markers in human serum.

  E N Savvateeva, E I Dementieva, M V Tsybulskaya, T V Osipova, T P Ryabykh, A Yu Turygin, R A Yurasov, A S Zasedatelev, A Yu Rubina
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  ABSTRACT: The biochip was constructed for simultaneous assay of total and free prostate-specific antigen, alpha-fetoprotein, cancer embryonic antigen, human chorionic gonadotropin, and neuron-specific enolase. These biochips represent an array of gel elements with covalently immobilized proteins. The major analytic characteristics of the developed method were obtained. It was shown that the results of simultaneous assay of six tumor markers in blood serum well correlated with routine measurement of each marker using enzyme immunoassay kits. This approach allowed us to reveal the hook effect of high concentrations during biochip assay, which prevents distortion of the diagnostic picture at high concentration of the analyte in the sample.
  Bulletin of Experimental Biology and Medicine 06/2009; 147(6):737-41. · 0.27 Impact Factor
• Article: Diagnosis of cancer using microchip-based system for simultaneous quantitation of six tumor markers.

  T P Ryabykh, Z A Sokolova, N B Paklin, T V Osipova
  Doklady Biochemistry and Biophysics 437:98-101. · 0.33 Impact Factor
• Article: [Development of a biochip for quantitative determination of two forms of prostate specific antigen using internal calibration curve].

  E V Konovalova, E N Savvateeva, E I Dement'eva, M A Filippova, A Iu Turygin, T V Osipova, T P Riabykh, A Iu Rubina, A S Zasedatelev
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  ABSTRACT: Three-dimensional gel-based microchip allowing simultaneous quantitative detection of total (PSAtot) and free (PSAfree) forms of prostate specific antigen in human serum (in a format "one patient-one biochip") was developed. A method, which doesn't require preliminary construction of calibration curves when performing an assay, was applied for quantitative determination of PSAtot and PSAfree. Gel elements with immobilized antigen (PSA) in different concentration, forming an internal calibration curve, were included in a structure of the microchip, in addition to the elements with immobilized antibodies specific against PSAtot and PSAfree. The specialized software "ImaGelAssay" was used for data processing and interpretation. The sensitivity of the assay performed on biochips was 0.3 ng/ml for PSAtot and 0.2 ng/ml for PSAfree. Variation coefficient for the measurements inside one series of microchips didn't exceed 10%. Correlation coefficient between the results of measurements in human sera obtained on biochips and by the standard ELISA method was 0.988 for PSAtot and 0.987 for PSAfree.
  Molekuliarnaia biologiia 41(4):734-8.
• Article: Protein microchips in quantitative assays for tumor markers.

  E I Dementieva, A Yu Rubina, E L Darii, V I Dyukova, A S Zasedatelev, T V Osipova, T P Ryabykh, A Yu Baryshnikov, A D Mirzabekov
  Doklady Biochemistry and Biophysics 395:88-92. · 0.33 Impact Factor