Matthias Hermes

Technische Universität Dortmund, Dortmund, North Rhine-Westphalia, Germany

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Publications (56)200.36 Total impact

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    ABSTRACT: The production of ultra-light, economical and ecologically oriented component structures with high contour complexity in the automotive industry necessitates the use of profiles and tubes made of innovative high-strength steel grades. The torque superposed spatial (TSS) profile bending process, a kinematic process to bend profiles with complex three-dimensional bending contours, and the incremental tube forming (ITF) process, a kinematic process to produce three-dimensionally shaped tubes with varying cross-sectional diameters over their longitudinal axis, both offer the flexible production of structural components in small or medium batch sizes. The aim of the ProTuBend project was to advance the two innovative processes for industrial use to allow forming and bending of load-optimised 3D tubes and profiles made of high- and ultra-high-strength steel. Investigations of process limits of both processes were used to analyse the necessity of process extensions to meet the challenge of higher process forces and increased material failure because of lower formability. As a result, the TSS bending process was extended by additional driven bending axes that improved the positioning of the bending head and enabled the production of 3D structures with twisted components. The additional integration of an induction heating device enabled the direct production of a load optimised tractor cabin structure, the heating device being used to partially harden the structural components. For incremental tube forming, different tool geometries and tool positions were analysed and improved. A newly designed industrial bending machine allowed the direct production of a load- and weight-optimised automotive seat frame member.
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    ABSTRACT: Engineering research, in the field of light weight design, is strongly oriented towards the development of new high strength materials and innovative forming methods, capable of withstanding limitations with regard to the wide variety of technological and economical aspects. Cost effective lightweight construction, in addition to the reduction of energy/material consumption and overall reduction of weight, also strongly depends on stability, continuity and robustness of production processes. Kinematic solutions for the production of spatial designed structures, in terms of 3D bending of profiles and tubes, show great potential in an increase of efficiency in the field of light weight design. The Torque Superposed Spatial bending method - TSS, developed at the Institute of Forming Technology and Lightweight Construction, Technische Universitat Dortmund, represents an innovative, robust and cost effective technical solution for 2D and 3D bending of tubes and profiles and offers a wide spectrum of capabilities, such as process continuity, parameter adaptation and flexibility for spatial bending of profiles with arbitrary cross-sections. In this paper, an introduction to 3D numerical analysis of the 2D profile bending method using TSS method is introduced and presented. The first objective of the work is to establish validity of the numerical model for the bending parameters, such as the bending force and bending momentum. Secondly, further investigations of the state of stresses and strains during load and unload conditions were performed. These are important for any further analysis and understanding of spring-back, residual stresses and cross section deformation of the profiles. The numerical simulations are performed with the use Abaqus software code, with elastic plastic material characteristics, and are, for the purpose of validation, compared to experimental data.
    Strojniski Vestnik 03/2013; 59(3):139-147. DOI:10.5545/sv-jme.2012.483 · 0.78 Impact Factor
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    ABSTRACT: Laboratory experiments play a significant role in engineering education. The main concern of the described hands-on miniLABs initiative (as a work in progress) is lowering the hurdles in order to provide engineering students with an informal and straightforward access to experiments carried out in labs of the IUL at TU Dortmund University. miniLABs will offer students different, short and voluntary hands-on lab sessions, consisting of two different modes and different aspects related to manufacturing technology in the field of forming processes. In small teams, students can get in touch with practical engineering activities in the fields of present scientific research, either to study a certain phenomenon or to look at a wider engineering context. Based on the framework of experiential learning, miniLABs tries to foster the shift from teaching to deep learning. Finally, this initiative aims to inspire young students for real and hands-on engineering experiments and to contribute to the science education of these young and future engineers.
    Global Engineering Education Conference (EDUCON), 2013 IEEE; 01/2013
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    ABSTRACT: Biomechanical properties of tumor cells play an important role for the metastatic capacity of cancer. Cellular changes of viscoelastic features are prerequisite for cancer progression since they are essential for proliferation and metastasis. However, only little is known about the way how expression of oncogenes influences these biomechanical properties. To address this aspect we used a breast cancer cell line with inducible expression of an oncogenic version of ERBB2. ERBB2 is known to be correlated with bad prognosis in breast cancer. Cell elasticity was determined by the Optical Stretcher, where suspended cells are deformed by two slightly divergent laser beams. We found that induction of ERBB2 caused remarkable biomechanical alterations of the MCF-7 cells after 24 h: the cells actively contracted in response to mechanical stimuli, a phenomenon known as mechanoactivation. After this period, as the cells became senescent, the mechanoactivity returned to control levels. Time-resolved gene array analysis revealed that mechanoactivation was accompanied by temporal upregulation of 46 cytoskeletal genes. A possible role of these genes in tumor progression was investigated by expression analyses of 766 breast cancer patients. This showed an association of 12 out of these 46 genes with increased risk of metastasis. Our results demonstrate that overexpression of ERBB2 causes mechanoactivation of tumor cells, which may enhance tumor cell motility fostering distant metastasis.
    Cytoskeleton 05/2012; 69(5):267-77. DOI:10.1002/cm.21023 · 3.01 Impact Factor
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    ABSTRACT: Incremental tube forming (ITF) is a new process allowing a flexible manufacturing of 2D and 3D bent tubes with load-optimized cross sections by means of the combination of the procedures spinning and bending. The aim of this paper is to acquire an in-depth process understanding concerning the surface roughness. This paper focuses on the spinning process operation of the ITF process. The influence of the spinning roll geometry and the process parameters on the theoretical surface roughness is studied in detail. Crest height h and roughness average parameter R a are formulated as function of process parameters and spinning roll geometry. Also, a fishbone diagram with the parameters influencing the tube surface characteristics is provided. Experiments are performed to quantify the divergences of the equations. The theoretical approach can be used to understand the incremental tube forming process in more detail.
    Production Engineering 04/2012; 7(2-3). DOI:10.1007/s11740-012-0424-4
  • Steel Research International 01/2012; · 1.02 Impact Factor
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    ABSTRACT: Alterations in lipid metabolism and in the lipid composition of cellular membranes are linked to the pathology of numerous diseases including cancer. However, the influence of oncogene expression on cellular lipid profile is currently unknown. In this work we analyzed changes in lipid profiles that are induced in the course of ERBB2-expression mediated premature senescence. As a model system we used MCF-7 breast cancer cells with doxycycline-inducible expression of NeuT, an oncogenic ERBB2 variant. Affymetrix gene array data showed NeuT-induced alterations in the transcription of many enzymes involved in lipid metabolism, several of which (ACSL3, CHPT1, PLD1, LIPG, MGLL, LDL and NPC1) could be confirmed by quantitative realtime PCR. A study of the glycerophospholipid and lyso-glycerophospholipid profiles, obtained by high performance liquid chromatography coupled to Fourier-transform ion cyclotron resonance-mass spectrometry revealed senescence-associated changes in numerous lipid species, including mitochondrial lipids. The most prominent changes were found in PG(34:1), PG(36:1) (increased) and LPE(18:1), PG(40:7) and PI(36:1) (decreased). Statistical analysis revealed a general trend towards shortened phospholipid acyl chains in senescence and a significant trend to more saturated acyl chains in the class of phosphatidylglycerol. Additionally, the cellular cholesterol content was elevated and accumulated in vacuoles in senescent cells. These changes were accompanied by increased membrane fluidity. In mitochondria, loss of membrane potential along with altered intracellular distribution was observed. In conclusion, we present a comprehensive overview of altered cholesterol and glycerophospholipid patterns in senescence, showing that predominantly mitochondrial lipids are affected and lipid species less susceptible to peroxidation are increased.
    Biochimica et Biophysica Acta 12/2011; 1821(9):1256-68. DOI:10.1016/j.bbalip.2011.11.008 · 4.66 Impact Factor
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    ABSTRACT: Alterations in lipid metabolism and in the lipid composition of cellular membranes are linked to the pathology of numerous diseases including cancer. However, the influence of oncogene expression on cellular lipid profile is currently unknown. In this work we analyzed changes in lipid profiles that are induced in the course of ERBB2-expression mediated premature senescence. As a model system we used MCF-7 breast cancer cells with doxycycline-inducible expression of NeuT, an oncogenic ERBB2 variant. Affymetrix gene array data showed NeuT-induced alterations in the transcription of many enzymes involved in lipid metabolism, several of which (ACSL3, CHPT1, PLD1, LIPG, MGLL, LDL and NPC1) could be confirmed by quantitative realtime PCR. A study of the glycerophospholipid and lyso-glycerophospholipid profiles, obtained by high performance liquid chromatography coupled to Fourier-transform ion cyclotron resonance-mass spectrometry revealed senescence-associated changes in numerous lipid species, including mitochondrial lipids. The most prominent changes were found in PG(34:1), PG(36:1) (increased) and LPE(18:1), PG(40:7) and PI(36:1) (decreased). Statistical analysis revealed a general trend towards shortened phospholipid acyl chains in senescence and a significant trend to more saturated acyl chains in the class of phosphatidylglycerol. Additionally, the cellular cholesterol content was elevated and accumulated in vacuoles in senescent cells. These changes were accompanied by increased membrane fluidity. In mitochondria, loss of membrane potential along with altered intracellular distribution was observed. In conclusion, we present a comprehensive overview of altered cholesterol and glycerophospholipid patterns in senescence, showing that predominantly mitochondrial lipids are affected and lipid species less susceptible to peroxidation are increased.
    Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 01/2011; · 4.50 Impact Factor
  • 6. IGIP Regionalkonferenz, Renaissance der Ingenieurpädagogik -- Entwicklungslinien im europäischen Raum. Referat der 6. IGIP Regionalkonferenz; 01/2011
  • Toxicology Letters 07/2010; 196. DOI:10.1016/j.toxlet.2010.03.276 · 3.36 Impact Factor
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    ABSTRACT: The purpose of this work was to study the prognostic influence in breast cancer of thioredoxin reductase 1 (TXNRD1) and thioredoxin interacting protein (TXNIP), key players in oxidative stress control that are currently evaluated as possible therapeutic targets. Analysis of the association of TXNRD1 and TXNIP RNA expression with the metastasis-free interval (MFI) was performed in 788 patients with node-negative breast cancer, consisting of three individual cohorts (Mainz, Rotterdam and Transbig). Correlation with metagenes and conventional clinical parameters (age, pT stage, grading, hormone and ERBB2 status) was explored. MCF-7 cells with a doxycycline-inducible expression of an oncogenic ERBB2 were used to investigate the influence of ERBB2 on TXNRD1 and TXNIP transcription. TXNRD1 was associated with worse MFI in the combined cohort (hazard ratio = 1.955; P < 0.001) as well as in all three individual cohorts. In contrast, TXNIP was associated with better prognosis (hazard ratio = 0.642; P < 0.001) and similar results were obtained in all three subcohorts. Interestingly, patients with ERBB2-status-positive tumors expressed higher levels of TXNRD1. Induction of ERBB2 in MCF-7 cells caused not only an immediate increase in TXNRD1 but also a strong decrease in TXNIP. A subsequent upregulation of TXNIP as cells undergo senescence was accompanied by a strong increase in levels of reactive oxygen species. TXNRD1 and TXNIP are associated with prognosis in breast cancer, and ERBB2 seems to be one of the factors shifting balances of both factors of the redox control system in a prognostic unfavorable manner.
    Breast cancer research: BCR 06/2010; 12(3):R44. DOI:10.1186/bcr2599 · 5.88 Impact Factor
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    ABSTRACT: Only little is known about how cells coordinately behave to establish functional tissue structure and restore microarchitecture during regeneration. Research in this field is hampered by a lack of techniques that allow quantification of tissue architecture and its development. To bridge this gap, we have established a procedure based on confocal laser scans, image processing, and three-dimensional tissue reconstruction, as well as quantitative mathematical modeling. As a proof of principle, we reconstructed and modeled liver regeneration in mice after damage by CCl(4), a prototypical inducer of pericentral liver damage. We have chosen the regenerating liver as an example because of the tight link between liver architecture and function: the complex microarchitecture formed by hepatocytes and microvessels, i.e. sinusoids, ensures optimal exchange of metabolites between blood and hepatocytes. Our model captures all hepatocytes and sinusoids of a liver lobule during a 16 days regeneration process. The model unambiguously predicted a so-far unrecognized mechanism as essential for liver regeneration, whereby daughter hepatocytes align along the orientation of the closest sinusoid, a process which we named "hepatocyte-sinusoid alignment" (HSA). The simulated tissue architecture was only in agreement with the experimentally obtained data when HSA was included into the model and, moreover, no other likely mechanism could replace it. In order to experimentally validate the model of prediction of HSA, we analyzed the three-dimensional orientation of daughter hepatocytes in relation to the sinusoids. The results of this analysis clearly confirmed the model prediction. We believe our procedure is widely applicable in the systems biology of tissues.
    Proceedings of the National Academy of Sciences 06/2010; 107(23):10371-6. DOI:10.1073/pnas.0909374107 · 9.81 Impact Factor
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    ABSTRACT: Members of the Bcl-2 family act as master regulators of mitochondrial homeostasis and apoptosis. We analyzed whether ERBB2 influences the prognosis of breast cancer by influencing the proapoptotic versus antiapoptotic balance of Bcl-2 family members. ERBB2-regulated Bcl-2 family members were identified by inducible expression of ERBB2 in MCF-7 breast cancer cells and by correlation analysis with ERBB2 expression in breast carcinomas. The prognostic relevance of ERBB2-regulated and all additional Bcl-2 family members was determined in 782 patients with untreated node-negative breast cancer. The biological relevance of ERBB2-induced inhibition of apoptosis was validated in a murine tumor model allowing conditional ERBB2 expression. ERBB2 caused an antiapoptotic phenotype by upregulation of MCL-1, TEGT, BAG1, BNIP1, and BECN1 as well as downregulation of BAX, BMF, BNIPL, CLU, and BCL2L13. Upregulation of the antiapoptotic MCL-1 [P = 0.001, hazard ratio (HR) 1.5] and BNIP3 (P = 0.024; HR, 1.4) was associated with worse prognosis considering metastasis-free interval, whereas clusterin (P = 0.008; HR, 0.88) and the proapoptotic BCL2L13 (P = 0.019; HR, 0.45) were associated with better prognosis. This indicates that ERBB2 alters the expression of Bcl-2 family members in a way that leads to adverse prognosis. Analysis of apoptosis and tumor remission in a murine tumor model confirmed that the prototypic Bcl-2 family member Bcl-x(L) could partially substitute for ERBB2 to antagonize tumor remission. Our results support the concept that ERBB2 influences the expression of Bcl-2 family members to induce an antiapoptotic phenotype. Antagonization of antiapoptotic Bcl-2 family members might improve breast cancer therapy, whereby MCL-1 and BNIP3 represent promising targets.
    Clinical Cancer Research 01/2010; 16(2):451-60. DOI:10.1158/1078-0432.CCR-09-1617 · 8.19 Impact Factor
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    ABSTRACT: It was previously demonstrated that the dipeptide carnosine inhibits growth of cultured cells isolated from patients with malignant glioma. In the present work we investigated whether carnosine also affects tumor growth in vivo and may therefore be considered for human cancer therapy. A mouse model was used to investigate whether tumor growth in vivo can be inhibited by carnosine. Therefore, NIH3T3 fibroblasts, conditionally expressing the human epidermal growth factor receptor 2 (HER2/neu), were implanted into the dorsal skin of nude mice, and tumor growth in treated animals was compared to control mice. In two independent experiments nude mice that received tumor cells received a daily intra peritoneal injection of 500 microl of 1 M carnosine solution. Measurable tumors were detected 12 days after injection. Aggressive tumor growth in control animals, that received a daily intra peritoneal injection of NaCl solution started at day 16 whereas aggressive growth in mice treated with carnosine was delayed, starting around day 19. A significant effect of carnosine on tumor growth was observed up to day 24. Although carnosine was not able to completely prevent tumor growth, a microscopic examination of tumors revealed that those from carnosine treated animals had a significant lower number of mitosis (p < 0.0003) than untreated animals, confirming that carnosine affects proliferation in vivo. As a naturally occurring substance with a high potential to inhibit growth of malignant cells in vivo, carnosine should be considered as a potential anti-cancer drug. Further experiments should be performed in order to understand how carnosine acts at the molecular level.
    Molecular Cancer 01/2010; 9(1):2. DOI:10.1186/1476-4598-9-2 · 5.40 Impact Factor
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    ABSTRACT: A new roll-based process and machine for three-dimensional bending of profiles with symmetrical and asymmetrical cross-sections have been developed. Compared to conventional processes like stretch bending, the advantage of the Torque Superposed Spatial (TSS) bending is the kinematic adjustment of the bending contour, leading to higher flexibility and cost efficiency, especially in small batch production. To define the spatial geometry of the workpiece, a torque is superposed to the bending moment. Results of the analytical and numerical investigations concerning the mechanics of deformation and the machine parameters of the new process are presented.
    CIRP Annals - Manufacturing Technology 01/2010; 59(1-1):315-318. DOI:10.1016/j.cirp.2010.03.017 · 2.54 Impact Factor
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    ABSTRACT: Recently, a genome-wide single nucleotide polymorphism association study has identified a sequence variant 30 kb upstream of the c-Myc gene (allele T of rs9642880) that confers susceptibility to bladder cancer. However, the role of exposure to bladder carcinogens has not been considered. This prompted us to analyse the relevance of this polymorphism in 515 bladder cancer cases and 893 controls where the quality and quantity of occupational exposure to bladder carcinogens has been documented. When we analysed a hospital-based case-control series not selected for occupational exposure, rs9642880[T] was influential, in contrast to GSTM1 0/0. However, in a case-control series of patients that have been occupationally exposed to aromatic amines and polycyclic aromatic hydrocarbons, rs9642880[T] was not influential but GSTM1 0/0 was significantly associated with bladder cancer risk. Therefore, the degree to which rs9642880[T] and GSTM1 0/0 confer susceptibility to urinary bladder cancer seems to depend on the extent of exposure to urinary bladder carcinogens.
    Pharmacogenetics and Genomics 10/2009; 19(11):903-6. DOI:10.1097/FPC.0b013e328331b554 · 3.45 Impact Factor
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    ABSTRACT: The paper deals with two new process variants invented by the authors. Both use the principle of stress superposition to reach an additional plastification of the material for a controlled influencing of the forming zone. By the superposition of an additional load on a forming process it is possible to compensate the springback effect e.g. of bending angles or bending radii to extend the forming limits of the material and to reduce the forming forces. The first process is the new process of incremental tube forming. This process is based on the combination of a spinning process with the free forming of tubular semi-finished products. This process is suitable to bend tubes two- and threedimensionally to an arbitrary contour and to manufacture tailored tubes. The process is useful to bend tubes without a die with full springback compensation. The second process is an advanced sheet metal air bending process using a roll which is pushed against the bending part after the loading operation and before unloading. This results in an incremental superposition of stresses along the bent part leading to a springback reduction and improvement of the crash properties. The paper shows the principles of both procedures and presents the results of the experimental investigations.
    International Journal of Material Forming 08/2009; 2:817-820. DOI:10.1007/s12289-009-0613-7 · 1.42 Impact Factor
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    ABSTRACT: This paper focuses on new forming techniques for the improvement of product quality, the reduction of process chains and the saving of our available resources. The presented and discussed technologies cover extrusion of reinforced profiles, bending of profiles, single point incremental forming of polymers, and tool systems made of polymers coated by hard metal as well as re-manufacturing strategies. Beside the process principles and advantages several results from numerical and experimental investigations will be given in order to show the motivation for fundamental and application oriented research in the field of forming technology.
    International Journal of Material Forming 08/2009; 2:29-38. DOI:10.1007/s12289-009-0656-9 · 1.42 Impact Factor
  • Chemistry and Physics of Lipids 08/2009; 160. DOI:10.1016/j.chemphyslip.2009.06.053 · 2.59 Impact Factor

Publication Stats

992 Citations
200.36 Total Impact Points

Institutions

  • 2007–2013
    • Technische Universität Dortmund
      • • Leibniz Research Centre for Working Environment and Human Factors
      • • Institute of Forming Technology and Lightweight Design
      Dortmund, North Rhine-Westphalia, Germany
  • 2007–2012
    • Leibniz Research Center for Working Enviroment and Human Factors
      Dortmund, North Rhine-Westphalia, Germany
  • 2004–2007
    • University of Leipzig
      • • Institute of Legal Medicine
      • • Rudolf Boehm Institute of Pharmacology and Toxicology
      Leipzig, Saxony, Germany
  • 2006
    • University of Porto
      • Faculdade de Farmácia
      Porto, Distrito do Porto, Portugal
  • 2003
    • Technische Universität Kaiserslautern
      • Department of Biology
      Kaiserlautern, Rheinland-Pfalz, Germany