Daniela Riggio

IEO - Istituto Europeo di Oncologia, Milano, Lombardy, Italy

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Publications (9)15.12 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Postoperative atrial fibrillation (AF) is a well-known complication occurring after thoracic surgery. B-type natriuretic peptide has recently been investigated as a predictive marker of postoperative AF after cardiac surgery. The aim of this study was to evaluate a definite cut-off for N-terminal pro-B type natriuretic peptide (NT-proBNP) in predicting postoperative AF in lung cancer patients. NT-proBNP was determined before and after surgery in 400 patients. Cardiac function was monitored by continuous postoperative ECG and clinical cardiological evaluation. AF occurred in 18% of the patients. Receiver operating characteristic curve analyses identified a cut-off of 182.3 ng l(-1) as the one with the highest sensitivity and specificity. Perioperative increased levels of NT-proBNP seem to predict postoperative AF in patients undergoing thoracic surgery, and a single cut-off of 182.3 ng l(-1) can be used to select high-risk patients who could receive preventive therapy, leading to a considerable decrease in the total costs associated with the management of this complication.
    Biomarkers 05/2010; 15(3):259-65. · 1.88 Impact Factor
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    ABSTRACT: HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 66 are considered carcinogenic for human beings. DNA-chip technology, Papillocheck HPV-screening (Greiner) and reverse dot blot, Linear Array (LA) (Roche) are tools to assess the distribution of HPV genotypes. The aim of the study was to compare the clinical performance of Papillocheck and LA assays using a clinical cut-off of CIN2+. The secondary aim was to comparatively assess the distribution of HPV types using these two assays. The study population comprised 239 women referred for colposcopy and histology. Papillocheck, LA, and Hybrid Capture II (HCII) tests were done on all samples. All tests showed good sensitivity and NPV (greater than 90%). None of the comparisons of sensitivities, specificities, PPVs, and NPVs showed statistically relevant differences between tests. High-risk HPV positivity rate was similar for all tests (Papillocheck 75%, LA 77%, and HCII 73%). Agreement between tests was good. The concordance levels between HCII and Papillocheck and between HCII and LA were 93% (k=0.82) and 92% (k=0.80), respectively. Papillocheck and LA tests showed a high overall concordance rate of 96% (k=0.90). HPV16 was the most detected type (45% with Papillocheck, and 47% with LA), and HPV31 was the second most detected type (13% with Papillocheck, and 14% with LA). The Papillocheck HPV-screening test and LA test have a good clinical sensitivity to detect HPV types in CIN2+ patients. These assays allow, in the same experiment, to detect and determine the virus type. Our study showed that HPV types 16 and 31/33 are the most prevalent.
    Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology 11/2009; 47(1):38-42. · 3.12 Impact Factor
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    ABSTRACT: Human papillomavirus (HPV) are distributed widely and persistent infection with high-risk (HR) HPV is recognized as a necessary cause of cervical cancer. The aim of this study was to evaluate the distribution of different HR-HPV genotypes in 199 women with cervical pre-invasive lesions undergoing conservative treatment. A Linear Array HPV Genotyping Test was used to identify individual HPV genotypes in cervical samples. It was observed that the most prevalent HPV genotypes were HPV 16 (52.6%), HPV 51 (13.5%), and HPV 31 (10.9%); HPV 18 was found in 7.3% of the patients. Stratifying the different HPV genotypes according to the severity of the cervical lesion, a strong association between the increasing severity of the histological diagnosis and the detection of more carcinogenic HR-HPV type was found, and in all but one cervical intraepithelial neoplasia of grade 3 the presence of at least one HR-HPV could be detected, with more than 70% of cervical intraepithelial neoplasia of grade 3 patients bearing HPV 16. Multiple infections, comprising between 2 and 6 HPV types, were found in 43% of patients; however, the presence of more than 1 HR-HPV type was not associated with an increased risk of high grade lesions. In conclusion, this data show that HPV 16, 51, 31, 52, and 18 were the prevalent types found in patients with cervical lesion undergoing conservative treatment, with a high prevalence of HPV 16 in cervical intraepithelial neoplasia of grade 3 patients. No association between multiple infection and severity of the lesion could be found.
    Journal of Medical Virology 03/2009; 81(2):271-7. · 2.37 Impact Factor
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    ABSTRACT: Prevention and surveillance programs are key to contain Nosocomial Infections (Nis). At the European Institute of Oncology, surveillance based on ex-post data collection has been done since the inception of hospital activity; laboratory-based surveillance of microbiological alert was not standardized. This study describes the issues related to the recent introduction into the hospital routine of a laboratory-based automated surveillance system and its clinical impact on monitoring and treatment of Nis. An interdisciplinary team defined the alerts and the actions to be taken in response; recipients of the alert messages were identified and software was programmed. Program features were created so their employment would generate a prompt notification of clinically critical results. After a training period, the program was introduced in the hospital routine. There were a total of 150 generated alerts; the main alert related to microorganisms requiring prompt patient isolation and/or public notification. Clinical use of the program was relevant in detection and immediate notification of Cytomegalovirus active infection in stem cell recipients and central venous catheter related candidemia: the prompt administration of adequate treatment was possible hours earlier compared to the previous approach. A laboratory-based automated surveillance system is effective in facilitating the management of Nis; its clinical employment also leads to important clinical advantages in patient care.
    ecancermedicalscience 01/2009; 3:137.
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    ABSTRACT: This study performed a retrospective analysis on the relationship between blood culture time-to-positivity (TP) and type of isolated microorganism, antibiotic administration, and immunological status of the patients. We analyzed the data related to 1,218 positive blood cultures. When compared to Gram positive bacteraemia, the percentage of Gram negative growth was higher and the mean TP significantly shorter (p < 0.0001). In patients receiving antibiotics, median and mean TPs of blood culture were different for Gram positive bacteraemia (log-rank p = 0.0022, Wilcoxon p < 0.0001) but not for Gram negative (log-rank p = 0.4011, Wilcoxon p = 0.1585). No statistically significant effect on TP was found for sampling site, interaction between sampling site and antibiotic administration, and immunological status of the patient. In conclusion, TP is independent of antibiotic therapy in cases of Gram negative bacteraemia, while for Gram positive bacteraemia a prolongation of TP occurs.
    European Journal of Clinical Microbiology 08/2008; 28(1):95-8. · 3.02 Impact Factor
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    ABSTRACT: The diagnostic utility of procalcitonin (PCT) and C-reactive protein (CRP) to discriminate between infective fever and fever due to inflammation was assessed in hemato-oncological patients treated with aggressive chemotherapy. Values of PCT and PCR measured on days -1, 0, 1, 3 and 5 of onset of fever were analyzed using longitudinal regression analysis. Of 236 febrile episodes in 166 patients, 39 were due to bacteremia, 62 to other infections and 135 were classified as fever of unknown origin. PCT concentration increased early only in bacteremia and other infections (p<0.001), with the highest levels at day +1. No different trends were noted in patients with low WBC count (<1,000/microl). CRP increased with a similar trend in all the three groups. PCT determination may contribute significantly to the management of hemato-oncological patients who experience febrile episodes.
    Anticancer research 01/2008; 28(5B):3061-5. · 1.71 Impact Factor
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    ABSTRACT: Catheter-related bloodstream infections are part of all nosocomial infections prevention and surveillance programs; in addition to periodic monitoring, continuous awareness and notification of sentinel events through the use of dedicated software are fundamental. In this paper we describe the impact of a software package based on microbiological alerts management on surveillance programs and therapy of CVC-related infections. The Virtuoso Plus™ software (Metafora Informatica Srl, Milan, Italy) was used to extract information from laboratory files, using a list of alerts previously defined and shared with clinicians. One year of activity generated 134 alerts; in particular, a Candida albicans strain was isolated from central and peripheral blood cultures of the same patient. The actions taken were prompt CVC removal and antifungal therapy administration. This study demonstrates that a nosocomial infections surveillance program based on microbiological alerts is also effective in facilitating the handling of patients with CVC-related infections.
    Nutritional Therapy and Metabolism 10/2007; 25(4):195-200.
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    ABSTRACT: CA 19-9 is a marker principally related to pancreatic and gall bladder cancer. Although its determination has no value in screening for these malignancies, it is used in post-operative monitoring and during chemotherapeutic treatment of confirmed disease. Measurements during follow-up must be comparable and must be performed with standard, validated methods. We compared four routinely used analytical systems for CA 19-9 determination: the Architect i2000 and AxSYM systems from Abbott Laboratories, the Elecsys 1010 from Roche Diagnostics, and the KRYPTOR system from Brahms Diagnostics. We evaluated the analytical performance of the four systems and compared measurements of CA 19-9 values, which covered the whole analytical range. The analytical performance and accuracy of the four systems were fairly good, but Passing-Bablok regression and mountain plots showed significant differences in CA 19-9 values measured with the four platforms. Our data indicate that during tumor follow-up, the use of the same system is appropriate to avoid the risk of a variation due to the method rather than the disease. Moreover, whenever a change in analytical equipment is required, careful analysis of CA 19-9 results must be undertaken.
    Clinical Chemistry and Laboratory Medicine 02/2007; 45(1):100-4. · 3.01 Impact Factor
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    ABSTRACT: Catheter-related bloodstream infections are part of all nosocomial infections preven-tion and surveillance programs; in addition to periodic monitoring, continuous awareness and no-tification of sentinel events through the use of dedicated software are fundamental. In this paper we describe the impact of a software package based on microbiological alerts management on surveil-lance programs and therapy of CVC-related infections. The Virtuoso Plus™ software (Metafora Informatica Srl, Milan, Italy) was used to extract informa-tion from laboratory files, using a list of alerts previously defined and shared with clinicians. One year of activity generated 134 alerts; in particular, a Candida albicans strain was isolated from central and peripheral blood cultures of the same patient. The actions taken were prompt CVC re-moval and antifungal therapy administration. This study demonstrates that a nosocomial infections surveillance program based on microbiologi-cal alerts is also effective in facilitating the handling of patients with CVC-related infections. (Nu-tritional Therapy & Metabolism 2007; 25: 195-200) KEY WORDS: CVC-related infections, Automated surveillance, Sentinel events, Alert
    Nu-ritional Therapy & Metabolism. 01/2007; 25:195-200.

Publication Stats

60 Citations
15.12 Total Impact Points

Institutions

  • 2007–2010
    • IEO - Istituto Europeo di Oncologia
      • Division of Laboratory Medicine
      Milano, Lombardy, Italy
  • 2009
    • Hôpital Ambroise Paré – Hôpitaux universitaires Paris Ile-de-France Ouest
      Billancourt, Île-de-France, France