M J Ashraf

Shiraz University of Medical Sciences, Shīrāz, Fars, Iran

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Publications (26)12.14 Total impact

  • Fanar, Lebanon, 2nd Stem Cell Educational and Medical Symposium (STEMS); 05/2014
  • 2nd Stem Cell Educational and Medical Symposium (STEMS), Fanar, Lebanon; 01/2014
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    ABSTRACT: Objectives Partial-thickness defects evolving in mature articular cartilage do not heal spontaneously. Tissue engineering has long been investigated to repair articular cartilage defects. The current study was designed to observe chondrogenic differentiation of adipose-derived stem cells (ASCs) to repair partial-thickness articular cartilage defects in non-weight bearing area in adult rabbit. Results The results showed that in treatment groups, at 8 weeks post-surgery, the defects were resurfaced with hyaline-like tissue, an ideal interface between the engineered cartilage, the adjacent normal cartilage and the underlying bone was observed. In contrast, at 4 weeks post-implantation was partially filled with repaired tissue, but only half of the repaired tissue was hyaline cartilage. In control groups, defects were only filled with fibrotic tissue. The findings showed significant differences in the quality of cartilage between ASCs-injected groups (both 4 and 8 weeks treatment groups) compared to control groups, particularly at 8 weeks treatment groups (Fig.1, B,D and F). Methods A partial-thickness cartilage defect (without penetration of the subchondral bone) was created by a 4 mm trephine treatment in the medial condyle of femur of 12 adult Dutch rabbits. Autologous mesenchymal stem cells harvested from subcutaneous adipose tissue of the same rabbit were injected intra-articularly. According to the time elapsed post-surgery and ASCs using in the cartilage defects, 4 groups were defined (treatment and control groups; 4 and 8 weeks post-surgery). The rabbits were sacrificed at 4 and 8 weeks after surgery for histological analysis. Healing of the defect was investigated histologically using haematoxylin and eosin, safranin-O staining and toluidine blue. Conclusion These findings suggest that it is feasible to repair articular cartilage defects by seeding autologous ASCs
    Royan International Twin Congress on Reproductive Biomedicine and Stem Cells Biology & Technology, Tehran.Iran; 01/2013
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    ABSTRACT: OBJECTIVES: Rhinomaxillary mucormycosis is a clinical manifestation of zygomycosis in solid-organ transplant recipients. Without proper diagnosis and treatment, rhino-orbital-cerebral zygomycosis, particularly a central nervous system disease, will develop with substantial complications. CASE REPORT: A 42-year-old man who had undergone renal transplant was admitted to our otolaryngology department with unilateral bloody nasal discharge. Mucormycosis was detected in the necrotic tissue of the maxillary sinus. Surgical ablation of the infected parts, along with antifungal treatment, restricted extension of the infection. CONCLUSIONS: Early detection of opportunistic infections in transplant recipients plays an important role in preventing dissemination. Fungal infections, including zygomycosis, should be considered in all solid-organ recipients, especially in persons with local unusual manifestations. Early diagnosis and successful treatment reduce mortality.
    Experimental and clinical transplantation : official journal of the Middle East Society for Organ Transplantation. 06/2012;
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    ABSTRACT: Adipose-derived stem cells have been reported as a novel candidate for the repair of cartilage injuries in vivo. In order to assess their differentiation ability, adipose-derived stem cells isolated from rabbit fat tissue were injected into the midportion of a surgically created rabbit ear auricle cartilage defect. After several months, the auricles were resected, histopathologically assessed and compared with a control group. Histopathological examination of auricles removed three, four and five months after injection showed islands of new cartilage formation at the site of the surgically induced defect. Six months after injection, we observed well-formed, mature cartilaginous plates that completely filled the defect in the native cartilage. In the control group, there was no significant growth of new cartilage. The results of this study suggest the great potential of adipose-derived stem cells to repair damaged cartilage tissue in vivo.
    The Journal of Laryngology & Otology 06/2012; 126(8):770-4. · 0.68 Impact Factor
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    ABSTRACT: In patients with ulcerative colitis (UC), the repeated cycle of injury and repair of intestinal mucosa has been reported to increase the risk of colon cancer. So, a safe and efficient therapy is required for the treatment and prophylaxis for the disease.This study aims to investigate the efficacy of Calendula officinalis extract in treatment of experimentally induced ulcerative colitis in dog animal model. During fall 2010, 10 out-bred female German dogs (1-2 years old; weighs of 20-25 kg) were enrolled. Ulcerative colitis was induced with 6% acetic acid as enema and method of treatment was retrograde (via enema) too by C. officinalis. Loose stools, diarrhea, gross bleeding and loss of body weight happened after administration of acetic acid and crypt damage, loss of epithelium, infiltration of inflammatory cells and depletion of goblet cells were noticed histologically. C. officinalis could successfully resolve the damages of UC. Treatment with C. officinalis can broaden the current therapy options for UC.
    Iranian Red Crescent medical journal. 12/2011; 13(12):884-90.
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    N Azarpira, M J Ashraf, B Khademi, N Asadi
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    ABSTRACT: Metastatic tumors to the nasal cavity and paranasal sinuses are far less common than primary cancer in this location. Review of the 2004-2010 pathology records of patients with sinonasal malignancy, revealed three cases with distant metastases to this region from primary sites in kidney, prostate and breast. The clinical presentation, histopathological findings and clinical outcome is presented for each patient. Metastases to the nose and paranasal sinuses usually respond poorly to treatment and have a poor prognosis. This report highlights the importance of suspecting metastases in patients with a previous history of malignancy. Therefore, thorough history and clinical evaluation is mandatory in any known/treated case of cancer.
    Indian Journal of Otolaryngology and Head & Neck Surgery 10/2011; 63(4):349-52.
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    ABSTRACT: Actinomycosis of the tonsils has been reported in a variable percentage of tonsil specimens by other authors. This study determines the incidence of actinomyces in the palatine tonsil and evaluates the clinical diagnoses and histopathological features of tonsillectomy specimens. In a retrospective study, 204 patients who had undergone tonsillectomy for recurrent tonsillitis (group A) and for sleep apnea without a history of recurrent tonsillitis (group B) were enrolled. The prevalence rate was significantly higher in the adult compared with the pediatric population. The prevalence of tonsillar actinomycetes colonization was higher in patients who had undergone tonsillectomy for recurrent tonsillitis (43.9%) than in patients who had undergone tonsillectomy for obstructive sleep apnea (26.3%). The prevalence did not differ by sex of patient. Histopathological analysis of resected tonsils did not show active tissue infection. There was a statistically significant relationship between the presence of actinomycosis and age, with a greater occurrence of actinomycosis in adult patients. Although actinomyces colonization is more prevalent in patients with recurrent tonsillitis than sleepdisordered breathing, but the presence of actinomyces does not indicate any active disease.
    Iranian Red Crescent medical journal. 07/2011; 13(7):499-502.
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    Eye (London, England) 06/2011; 25(9):1245-6. · 1.97 Impact Factor
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    Indian Journal of Pathology and Microbiology 01/2011; 54(2):415-7. · 0.68 Impact Factor
  • N Azarpira, M J Ashraf, M Shishegar
    Indian Journal of Pathology and Microbiology 01/2011; 54(2):424-5. · 0.68 Impact Factor
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    Ashraf MJ, Azarpira N, Gandomi M
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    ABSTRACT: Choristoma is a tumor-like mass consisting of tissues foreign to the site at which they are located. We report an18 years old male presenting with persistent tonsillitis. Histological examination demonstrated the unexpectedpresence of a mature island of hyaline cartilage surrounded by lymphoid hyperplasia.
    Iranian Red Crescent Medical Journal. 01/2010;
  • Middle East Journal. 01/2010; 1.
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    ABSTRACT: We report the simultaneous occurrence of the tall cell variant of papillary carcinoma and squamous cell carcinoma in the thyroid gland. Histology and immunohistochemical findings are discussed and it seems that the malignant squamous component was transformed from the tall cell variant of papillary carcinoma. Metastasis from the squamous cell carcinoma is diagnosed in the regional lymph node.
    Indian Journal of Pathology and Microbiology 01/2010; 53(3):548-50. · 0.68 Impact Factor
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    Ashraf MJ, Azarpira N, Shishegar M
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    ABSTRACT: A16 year old boy was admitted to the hospital because of a swelling on the left side of mandible .He had multiplesmall indurated pearly papules on the head and neck with multiple well circumscribed radiolucent cystic space inmandible on panoramic radiography and CT-scan. Histopathologic studies of mandibular cyst and skin lesionsrevealed the finding in favor of nevoid basal cell carcinoma syndrome (NBCCS).
    Iranian Red Crescent Medical Journal. 01/2010;
  • Indian Journal of Pathology and Microbiology 01/2010; 53(4):906-7. · 0.68 Impact Factor
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    ABSTRACT: The need for pathologic examination of all tonsillectomy and/or adenoidectomy (T&A) specimens is controversial. Microscopic pathologic examination of these specimens is costly, but neglecting this step may miss diagnoses of significant diseases, especially malignancies. This study was designed to determine the rate of unexpected malignancies among patients who underwent T&A. All patients who underwent T&A at Shiraz University of Medical Sciences between February 2004 and February 2008 were included in a prospective study. Gross and microscopic pathologic examinations were done on all the specimens. The charts of the patients with significant pathological finding were reviewed. Information about pre-operative signs, symptoms, and risk factors were used to verify unexpected pathologic findings. A total of 5058 patients were included. The age distribution was 10 months to 92 years (mean 14.0 years). There were 2498 males (49.4) and 2560 females (50.6%). Significant pathological findings were detected in 54 patients (1%). One unexpected malignancy (0.019%) was found in an adult patient. No unexpected malignancies were found in pediatric patients. All T&A specimens in the adult population should be sent for microscopic pathological examination. Also specimens of nonroutine T&A in children (with positive findings in the medical history or on physical examination) should be sent for microscopic pathological examination. In children without positive findings in their history or on physical examination, gross pathological evaluation of routine T&A specimens by a pathologist is sufficient.
    International journal of pediatric otorhinolaryngology 10/2009; 73(11):1576-9. · 0.85 Impact Factor
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    ABSTRACT: To study fine needle aspiration cytology (FNAC) findings of tonsillar lesions with histological controls and to assess its role in the diagnostic evaluation of tonsillectomy specimens. This study consisted of 112 cases that required tonsillectomy, comprising 55 (49.1%) men and 57 (50.9%) women. The ages ranged between 20 and 62 years. The clinical diagnosis in 101 cases was chronic tonsillitis, whereas 11 were suspected of neoplasia. FNAC was performed before tonsillectomy under general or local anaesthesia or on fresh specimens using a 21-G needle. The smears were stained using Wright-Giemsa and Papanicolaou methods. Histological examination was carried out on surgical specimens of all cases and, when required, immunohistochemistry was performed on histological sections. The diagnostic outcomes between FNAC and surgical biopsy were compared. In this study, 106 cases were diagnosed as chronic tonsillitis/follicular hyperplasia, four cases as non-Hodgkin's lymphoma, one as Hodgkin's lymphoma and one as monophasic synovial sarcoma. All malignant cases were diagnosed by FNAC, but synovial sarcoma was incorrectly diagnosed as squamous cell carcinoma. Five cases clinically suspected of neoplasia were correctly diagnosed as chronic tonsillitis on cytology. Tonsillar aspiration is a safe procedure and is useful in the evaluation of tonsillectomy specimens. However, ancillary tests on cytological material are often needed when neoplasia is suspected and would help clinical management and allow histological examination of cases diagnosed cytologically as lymphoma.
    Cytopathology 04/2009; 21(3):170-5. · 1.71 Impact Factor
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    ABSTRACT: Background: Amniotic membrane transplantation (AMT) has been considered in combination with medicaltreatment in progressive infective keratitis. The purpose of this study was to evaluate the efficacy of AMT as anadjunctive treatment in the management of experimental pseudomonas keratitis.Methods: Cryopreserved AMT was performed on 12 pseudomonas rabbit corneal ulcers. After one week in onegroup amniotic membrane transplantation combined Poostchi Eye Research Center with topical medicaltreatment was done (AMT group). At the end of the second week, the clinical and pathological findings werecompared with those obtained from eight corneal ulcers in another group which had only been treated withtopical medication (control group).Results: There was not any significant difference in clinical signs between the two groups at the end of thesecond week. Corneal perforation was found in three cases of the control group but in none of the cases in theAMT group. Amniotic membranes were melted in four eyes, retracted in three eyes and intact in five eyes.Pathologic examination showed no significant difference in cellular infiltration or density of organism between thetwo groups.Conclusion: AMT is effective in preventing corneal perforation in the early stage of experimental pseudomonaskeratitis.
    Iranian Red Crescent Medical Journal. 01/2009;