Hüseyin Ozyurt

Gaziosmanpasa University, Dazimon, Tokat, Turkey

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Publications (28)48.03 Total impact

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    ABSTRACT: Recent studies have suggested the involvement of increased reactive oxygen species levels and decreased antioxidant system functions in psoriasis pathogenesis. In this study, we aimed to examine to investigate possible associations between the manganese superoxide dismutase (MnSOD Ala-9Val) and glutathione peroxidase (GPx1 Pro198Leu) polymorphisms and psoriasis susceptibility and disease progression in a Turkish population. The study group consisted of 100 unrelated patients with psoriasis and 167 unrelated healthy controls. Genomic DNA was extracted from peripheral leukocytes of whole blood which were obtained from all patients and control subjects. Genotyping was performed to identify MnSOD Ala-9Val and GPx1 Pro198Leu polymorphisms by a method based on PCR amplification and detection of polymorphisms with hybridization probes labeled with fluorescent dyes. Genotype and allele frequencies were compared between patients with psoriasis and 106 healthy control subjects. There was no significant difference between the MnSOD Ala-9Val single nucleotide polymorphism (SNP) genotype distributions and allele frequencies of the psoriasis patients and the control group (p = 0.99 and p = 0.89, respectively). There was also no significant difference between distributions of the genotype or allele frequencies of the GPx1 Pro198Leu SNP of the patient groups and control subjects (p = 0.99 and p = 0.96, respectively). Also, no significant difference was found between clinical severity of psoriasis and MnSOD Ala-9Val and GPx1 Pro198Leu polymorphism. This is the first report investigating the possible associations between the MnSOD Ala-9Val and GPx1 Pro198Leu polymorphisms and psoriasis susceptibility and disease progression in the Turkish population even if no significant difference was found between patient groups and control subjects. Further studies with large cohort on different populations and ethnicities will be able to better clarify the association.
    Archives for Dermatological Research 11/2013; · 2.71 Impact Factor
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    ABSTRACT: Abstract Background: Melasma is an acquired skin disease characterized clinically by development of gray-brown macules or patches. The lesions have geographic borders and most often seen on face and less frequently on the neck and forearms. Pathogenesis has not been completely understood yet. Although the disease constitutes a very disturbing cosmetic problem, it has not obtained an efficient treatment. There were not any studies in the literature that evaluates the role of oxidative stress in melasma. Objectives: The evaluation of the role of oxidative stress in melasma. Methods: Fifty melasma patients and 50 healthy volunteers were included in the study. The diagnosis was made clinically and the patients were evaluated by Melasma Area Severity Index. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) enzyme activities and malondialdehyde, nitric oxide, protein carbonyl levels were measured both in the melasma group and the control group. Results: SOD and GSH-Px enzyme activities were significantly higher in the patient group in comparison with the control group (p < 0.001). Protein carbonyl levels were significantly lower in the patient group (p < 0.001). Conclusion: The results show that the balance between oxidant and anti-oxidants was disrupted and the oxidative stress increased in melasma. These results improve the understanding of etiology-pathogenesis of the disease and its treatment.
    Cutaneous and Ocular Toxicology 10/2013; · 1.04 Impact Factor
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    ABSTRACT: Abstract Background: The conflicting information related to oxidative stress status in patients with chronic idiopathic urticaria has been reported in several studies. However, the association between acute urticaria (AU) and oxidative stress has not been investigated exhaustively. Objectives: To evaluate the role of the oxidative stress in the patients with AU by determining the oxidant/antioxidant activity in AU and to establish its clinical significance. Methods: About 50 patients with AU, (10 males, 40 females) and 30 unrelated healthy controls (4 males, 26 females) were enrolled into the study. The activity of the antioxidant enzymes copper-zinc superoxide dismutase (Cu-Zn-SOD), glutathione peroxidase (GSH-Px) and catalase, and the levels of malondialdehyde (MDA), serum NO and protein carbonyls levels in the plasma were measured spectrophotometrically at samples. Results: A statistically significant increase was observed in serum Cu-ZnSOD activities of the patients when compared with that of the controls (p < 0.001), while a statistically significant decrease was observed in GSH-Px activities of the patients according to the controls (p = 0.002). Serum MDA and NO levels were significantly higher in patients with AU when compared with control group (p < 0.001 for both of them). The levels of protein carbonyls were significantly lower in patients with AU when compared with control group (p < 0.001). Conclusion: It seems there is an oxidative burden in the patients with AU. Cutaneous oxidative stress may play a role in pathogenesis of the disease.
    Cutaneous and Ocular Toxicology 07/2013; · 1.04 Impact Factor
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    ABSTRACT: To determine if the paraoxonase 1 L55M and paraoxonase 1 Q192R gene polymorphisms have an effect on the risk of having a retinal vein occlusion (RVO). This case-control prospective study included 120 patients with RVO and 84 control subjects. All subjects were screened for age, gender, hypertension, diabetes, body mass index, fibrinogen, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides, total cholesterol, and very low-density lipoprotein. Subjects were also questioned about their smoking habits. Genomic DNA was extracted from peripheral leukocytes from EDTA anticoagulated blood. Genotyping of the paraoxonase 1 L55M and paraoxonase 1 Q192R polymorphisms was performed using real-time PCR. The frequency of the paraoxonase 1 (PON1) 55 LL genotype was significantly lower in patients with RVO than in the control subjects (28% versus 55%; p=0.005). Logistic regression analyses were also conducted. After adjusting for gender, diabetes, hypertension, plasma fibrinogen levels, and high-density lipoprotein cholesterol, the lower LL genotype was found to be an independent predictor of RVO (β=1.755; odds ratio=5.783; p<0.001; 95% confidence interval=2.579-12.967). Subjects with a lower frequency PON1 55 LL genotype had a higher risk of RVO. These results indicate that paraoxonase gene polymorphisms may be a possible risk factor for RVO. We suggest that the LL genotype may have a protective role in the pathogenesis of RVO in the Turkish population.
    Molecular vision 01/2013; 19:486-xxx. · 1.99 Impact Factor
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    HK J Paediatr (New Series). 01/2011; 16(1):3-8.
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    ABSTRACT: The goal of asthma treatment is to achieve clinical control. The aim of this study was to compare the role of measurement of nitric oxide and its products in exhaled breath condensate and asthma control questionnaire (ACQ), level of asthma control (LAC) according to GINA guidelines and bronchial provocation test (BPT) in assessing asthma control in cases with stable asthma. Thus, 47 patients with the diagnosis of stable asthma and 42 individuals in the control group were enrolled in the study. The mean ages of the patient and the control groups were 44 +/- 11 and 47 +/- 11 years, respectively. While there was no significant relation between LAC and levels of nitric oxide, nitrite and nitrate, there was a significant relation between ACQ and mini quality of life questionnaire (p< 0.001). In the group with positive BPT, ACQ scores and absolute serum eosinophil values were significantly higher (p< 0.05), and FEV(1)/FVC and PEF percentages were significantly lower than those of the group with negative BPT (p< 0.05). Reversibility was significantly higher in participants with positive BPT than in those with negative BPT (11.2 +/- 7.4 and 6.9 +/- 6.6, respectively; p< 0.05). There was no significant relationship between nitric oxide, nitrite, nitrate in the exhaled breath condensate and ACQ, LAC, BPT and pulmonary function tests (p> 0.05). In conclusion, it was found that the levels of exhaled breath condensate nitric oxide, nitrite and nitrate were not sufficient for determining the level of asthma control in patients with stable asthma. It was concluded that the currently available asthma control questionnaires may be adequate for assessing asthma control.
    Tuberkuloz ve toraks 01/2010; 58(1):5-15.
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    ABSTRACT: To evaluate oxidative damage to the macromolecules, including protein, lipid, and DNA, in association with total oxidation status (TOS), total antioxidant capacity (TAC) in patients with exudative age-related macular degeneration (AMD). Serum levels of malondialdehyde (MDA) as an index of lipid peroxidation, protein carbonyl (PC) as a marker of protein oxidation, 8-hydroxy-29-deoxyguanosine (8-OHdG) as an indicator of oxidative DNA damage along with TOS, TAC were measured by specific methods in 47 patients with exudative AMD and 25 age- and sex-matched healthy subjects. Significantly higher MDA, PC, 8-OHdG, TOS, and lower TAC levels were detected in the serum of patients with exudative AMD compared with their controls (p < 0.001). The results indicate that an imbalance between TOS and TAC leads to not only increased lipid damage, but also protein and DNA damage. These first reported results suggest that protein and DNA damage might also play a role in the pathogenesis of AMD.
    Current eye research 12/2009; 34(12):1089-93. · 1.51 Impact Factor
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    ABSTRACT: Viral hepatitides are considered a major health problem worldwide. There are only a few studies relevant to the epidemiology of these types of infection in the normal healthy population. In this study, we aimed to determine the seroprevalence of hepatitis B and hepatitis C as well as the frequency of isolated anti-HBc IgG positivity among a normal healthy population in a northern province of Turkey. This study was conducted in 70 areas (12 urban and 58 rural) in the province of Tokat, which is in the Black Sea region of Turkey, with about 530,000 inhabitants 18 years and older. All urban regions and some rural regions selected by a cluster sampling method were included in the study. The study population of 1,095 subjects (541 male and 554 female; urban 555 and rural 540) was selected by a random sampling method among 530,000 individuals. All individuals were tested for HBsAg, anti- HBs, anti-HBc IgG, anti-HCV, and alanine aminotransferase. The mean age of all participants was 41.4+/-17 years (range, 18-95). HBsAg, anti-HBs, anti-HBc IgG only, isole and anti-HCV were detected in 60 (5.5%), 250 (22.8%), 132 (12.1%), and 23 (2.1%) individuals of the 1,095 total participants, respectively. We did not find statistically significant differences between hepatitis B and C markers for men versus women or those living in rural versus urban areas. The rate of HBsAg positivity in individuals with a history of marriage to close relatives was higher. We found that the seroprevalences of hepatitis B and C in a northern province of Turkey are similar to the averages reported in other studies that were conducted in a different region of our country. The history of marriage to close relatives was associated with hepatitis B.
    The Turkish journal of gastroenterology: the official journal of Turkish Society of Gastroenterology 04/2009; 20(1):27-30. · 0.48 Impact Factor
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    ABSTRACT: This study was designed to investigate effect of gradual detorsion on testicular ischemia reperfusion injury. A total of 21 male rats were divided into 3 groups, each containing 7 rats. Torsion was created by rotating the left testis 720 degrees in a clockwise direction. Group 1 underwent sham operation. Group 2 (sudden detorsion) served as a torsion/detorsion group, receiving 2 hours torsion and 2 hours detorsion. In group 3, 360 degrees detorsion was done for 20 minutes after 720 degrees torsion for 2 hours. Then, testis was done full detorsion for 100 minutes. At the end of the experiments (fourth hour), left orchiectomy was performed to measure the tissue levels of malondialdehyde (MDA), superoxide dismutase, and glutathione peroxidase and to perform histologic examination in testes. The MDA levels of testis tissues were significantly increased in the sudden detorsion group as compared with the sham group. We found decrease of the MDA level in gradual detorsion group, but it was not a statistically significant amount. Significant decrease was found in the superoxide dismutase and glutathione peroxidase activities in the sudden detorsion group as compared with the sham and gradual detorsion groups. Histologic examinations were in accordance with the testicular tissue MDA levels. In the light of our biochemical and histopathologic findings, we can say that gradual detorsion has a trend to decrease the degree of testicular reperfusion injury in the rat torsion/detorsion model.
    Journal of Pediatric Surgery 11/2008; 43(10):1879-84. · 1.38 Impact Factor
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    ABSTRACT: The objective of our study was to elucidate serum levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP) in Behcet's disease (BD) patients with active and inactive period. The multicenter study included 53 patients with active (n = 28) and inactive (n = 25) BD (mean age, 34.3 +/- 9 years; 15 men and 38 women) satisfying the International Study Group criteria and 26 healthy controls (mean age, 34.4 +/- 6.1 years; seven men and 19 women) matched for age and gender from a similar ethnic background. Serum natriuretic peptides levels were determined by enzyme immunoassay kit. Mean serum ANP concentrations in the active patients (4.01 +/- 1.21 ng/ml) were significantly lower than in the healthy controls (5.76 +/- 1.99 ng/ml, p = 0.004). Mean serum BNP levels were found to be significantly higher in both the active (6.19 +/- 2.97 ng/ml) and inactive (6.49 +/- 2.88 ng/ml) BD groups compared with the control group (3.82 +/- 1.1 ng/ml, p = 0.004 and p = 0.001, respectively). Mean serum CNP concentrations in the active patients (0.49 +/- 0.12 ng/ml) were significantly lower than in the inactive patients (0.65 +/- 0.2 ng/ml, p = 0.017) and the healthy controls (0.8 +/- 0.27 ng/ml, p < 0.001). Our results suggest that changes in natriuretic peptide levels may be associated with vasculitis that play role in the etiopathogenesis of the BD.
    Clinical Rheumatology 05/2008; 27(9):1153-8. · 2.04 Impact Factor
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    ABSTRACT: We determined serum nitric oxide (NO) levels in patients with head and neck squamous cell carcinoma (SCC) and sought correlations with TNM staging, tumor localization, and tumor grade. Serum samples were obtained from 36 patients (mean age 63 years; range 37 to 80 years) with head and neck SCC prior to treatment and from 20 healthy individuals (mean age 56 years; range 30 to 72 years) as controls. Tumor staging was based on the criteria of the American Joint Committee of Cancer staging system in 2002. Thirteen patients had stage I-II, and 23 patients had stage III-IV tumors and all had well- or moderately-differentiated SCC (grade 1-2). Serum NO levels were analyzed by a spectrophotometric method based on the determination of total nitrite levels in serum and compared between the patient and control groups. The mean serum NO levels were 20.08+/-1.40 micromol/l and 13.57+/-0.99 micromol/l in cancer patients and controls, respectively (p=0.001). There were no correlations between NO levels and age, sex, tumor stage, localization, and histological grade. These data suggest that head and neck SCC is associated with increased serum NO levels, which may play a role in tumor growth.
    Kulak burun bogaz ihtisas dergisi: KBB = Journal of ear, nose, and throat 02/2007; 17(3):148-51.
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    ABSTRACT: The aim of this study was to investigate the neurotoxicity of formaldehyde on hippocampus and the protectiveeffects of caffeic acid phenethyl ester (CAPE) against these toxic effects. For this purpose, 21 male Wistarrats were divided into three groups. The rats in Group I comprised the controls, while the rats in Group IIwere injected every day with formaldehyde (FA). The rats in Group III received CAPE daily while exposed toformaldehyde. At the end of 8 days experimental period, all rats were sacrificed by decapitation. The brainsof the rats were removed and the hippocampus tissues were obtained from all brain specimens. Some of thehippocampus tissue specimens were used for determination of superoxide dismutase (SOD), glutathioneperoxidase (GSH-Px) and malondialdehyde (MDA) levels. The remaining hippocampus tissue specimens wereused for light microscopic and immunohistochemical evaluation. The levels of SOD, GSH-Px and MDA were significantly increased in rats treated with formaldehyde comparedwith those of the controls. Furthermore, in the microscopic examination of this group, formation of apoptoticbodies, pycnotic cells, and vacuoler degeneration areas were observed. However, decreased biochemicalparameters were detected in the rats administered CAPE while exposed to formaldehyde. Additionally, cellulardamage caused by formaldehyde was decreased, and structural appearance was similar to that of the controlrats in this group. These biochemical and histological findings observed in all groups were also confirmed bythe immunohistochemical evaluation. It was determined that formaldehyde-induced neuronal damage in hippocampus was prevented by administrationof CAPE.
    Neuroanatomy. 01/2007;
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    ABSTRACT: To show the oxidative stress after cigarette smoke exposure in rat testis and to evaluate the effects of caffeic acid phenethyl ester (CAPE). Twenty-one rats were divided into three groups of seven. Animals in Group I were used as control. Rats in Group II were exposed to cigarette smoke only (4 x 30 min/d) and rats in Group III were exposed to cigarette smoke and received daily intraperitoneal injections of CAPE (10 micromol/kg x d). After 60 days all the rats were killed and the levels of nitric oxide (NO) and anti-oxidant enzymes such as superoxide-dismutase, catalase and glutathione peroxidase (GSH-Px) and the level of malondialdehyde were studied in the testicular tissues of rats with spectrophotometric analysis. There was a significant increase in catalase and superoxide-dismutase activities in Group II when compared to the controls, but the levels of both decreased after CAPE administration in Group III. GSH-Px activity was decreased in Group II but CAPE caused an elevation in GSH-Px activity in Group III. The difference between the levels of GSH-Px in Group I and Group II was significant, but the difference between groups II and III was not significant. Elevation of malondialdehyde after smoke exposure was significant and CAPE caused a decrease to a level which was not statistically different to the control group. A significantly increased level of NO after exposure to smoke was reversed by CAPE administration and the difference between NO levels in groups I and III was statistically insignificant. Exposure to cigarette smoke causes changes in the oxidative enzyme levels in rat testis, but CAPE can reverse these harmful effects.
    Asian Journal of Andrology 04/2006; 8(2):189-93. · 2.14 Impact Factor
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    ABSTRACT: Reactive oxygen species (ROS) have been suggested to play an important role in physiopathology of schizophrenia. The major intracellular antioxidant enzymes, copper-zinc superoxide dismutase in the cytoplasm and manganese superoxide dismutase (Mn-SOD) in the mitochondria, rapidly and specifically reduce superoxide radicals to hydrogen peroxide. Polymorphisms in the genes encoding antioxidant enzymes should therefore result in predisposition to schizophrenia. The present study was performed to assess whether there is a genetic association between a functional polymorphism (Ala-9Val) in the human Mn-SOD gene in schizophrenic patients (n=153) and healthy controls (n=196) using a PCR/RFLP method. Significant differences in the genotypic distribution between schizophrenics and controls were observed. Genotypic distribution with 14 (9.2%) Ala/Ala, 106 (69.3%) Ala/Val and 33 (21.6%) Val/Val subjects in schizophrenia was different from those of controls with 46 (23.5%), 83 (42.3%) and 67 (34.2%), respectively (p<0.0001). When the patients with schizophrenia were divided into the subgroups as disorganized, paranoid and residual, there was a significant difference in genotypic distribution among the subgroups (chi2=11.35, df=4, p=0.023). This association between -9Ala Mn-SOD allele and schizophrenia suggests that -9Ala variant may have a contribution in the physiopathogenesis of schizophrenia. Further investigations are warranted in larger populations with other susceptible genes that might be associated with schizophrenia.
    Progress in Neuro-Psychopharmacology and Biological Psychiatry 02/2005; 29(1):123-31. · 3.55 Impact Factor
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    ABSTRACT: The purpose of this study was to investigate iron (Fe), zinc (Zn), and copper (Cu) levels of aqueous humor, lens, and serum in nondiabetics and diabetics and to determine the effects of diabetes on Fe, Zn, and Cu contents in the lens. Fe, Zn, and Cu contents of aqueous humor, lens, and serum samples of 19 patients (9 nondiabetic patients with a mean age of 62.3 +/- 5.4 yr, and 10 diabetic patients with a mean age of 59.5 +/- 5.9 yr) were analyzed by atomic absorption spectrometry using a prospective study design. The lens levels of Cu in diabetic patients were significantly higher compared with nondiabetic patients (p = 0.02); however; there was no difference in the other elements (Zn, Fe; p = 0.28, p = 0.74, respectively). The levels of Fe, Zn, and Cu in the aqueous humor and serum of diabetic patients were not found to be statistically significant when compared to nondiabetics (p = 0.46, p = 0.11, p = 0.18, and p = 0.22, p = 0.43, p = 0.72, respectively). These results demonstrate that increased Cu content of the lens presumably has a greater association with the development of lens opacification in diabetics than Zn and Fe content.
    Biological Trace Element Research 02/2005; 108(1-3):33-41. · 1.31 Impact Factor
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    ABSTRACT: Pulmonary fibrosis (PF) induced by anticancerogenic bleomycin (BLM) is one of the more common side effects encountered during cancer treatment. It has been suggested in the last decades that the main responsible agent in PF is reactive oxygen species which were generated also in normal physiological conditions in the human body. In this experimental study, we investigated the preventive or attenuating effects of caffeic acid phenethyl ester (CAPE) that has been demonstrated to have anti-inflammatory, cytocytatic, anticancerogenic, antiprolipherative and antioxidant effects on BLM-induced PF. Thirty-six Sprague-Dawley rats were divided randomly into four groups as sham operation, BLM, BLM + vitamin E (vit E), and BLM + CAPE groups. BLM (7.5 mg/kg, single dose) was applied intratracheally, and CAPE and vit E intraperitoneally in the appropriate groups. At the end of the fibrosis processes, lung tissues were removed and the levels of tissues hydroxyproline (OH-proline), malondialdehyde (MDA) and NO as well as the activities of superoxide dismutase (SOD), catalase (CAT) and myeloperoxidase (MPO) were determined. Also, the weights of the rats were recorded at 7th and 14th days of the experiments. BLM application to the rats resulted in a significant increase in the OH-proline level as compared to the controls. Administration of CAPE and vit E led to the remarkable reduction of total lung OH-proline levels compared to the rats treated with BLM alone (p < 0.0001). There were a decreases in antioxidant enzyme (SOD and CAT) activities while an increase in MPO activity in BLM group was found vs. the control group (p < 0.0001). CAPE had a regulator effect on these parameters: the increase in CAT and SOD activities and the decrease in MPO activity were seen after CAPE application. NO, MDA and OH-proline levels were increased in BLM group vs. the control group. CAPE was more effective in decreasing the tissue levels of NO, MDA and OH-proline than vit E. MPO activity, as a good marker of neutrophil sequestration to the tissues, in the BLM group was decreased by CAPE approximately to the control group. We suggest that CAPE is more effective on the prevention of BLM-induced fibrosis via antioxidant and free radical scavenger properties than vit E at the doses used in the present study. CAPE has some attenuating effects on BLM-induced PF affecting both oxidant and antioxidant systems as well as neutrophils sequestration.
    Clinica Chimica Acta 02/2004; 339(1-2):65-75. · 2.85 Impact Factor
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    ABSTRACT: We have investigated the effect of caffeic acid phenethyl ester (CAPE) on cisplatin-induced nephrotoxicity in rats. Administration of a single dose of cisplatin resulted in the elevation of blood urea nitrogen and creatinine in serum, as well as nitric oxide in kidney tissue of rats. Cisplatin also caused reduction of catalase (P < 0.0001), superoxide dismutase (P = 0.149) and glutathrone peroxidase (P < 0.0001) activities in kidney tissue. Although cisplatin caused elevation in malondialdehyde levels and myeloperoxidase activities in kidney tissue, they were not statistically significant. Caffeic acid phenethyl ester was found to be protective against cisplatin-induced antioxidant enzyme reductions. Treatment with free-radical scavenger CAPE attenuated the increase in plasma blood urea nitrogen and kidney nitric oxide levels, and showed histopathological protection against cisplatin-induced acute renal failure. Extensive epithelial cell vacuolization, swelling, desquamation and necrosis were observed in the kidney of the cisplatin-treated rat. There were also larger tubular lumens in cisplatin-treated rats than those of the control and the CAPE groups. Caffeic acid phenethyl ester caused a marked reduction in the extent of tubular damage. It is concluded that administration of cisplatin imposes an oxidative stress to renal tissue and CAPE confers protection against the oxidative damage associated with cisplatin. This mechanism may be attributed to its free-oxygen-radical scavenging activity.
    Journal of Applied Toxicology 01/2004; 24(1):27-35. · 2.60 Impact Factor
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    ABSTRACT: It has been shown that reactive oxygen species (ROS) contribute to the onset and progression of osteoarthritis. We investigated cartilage destruction and oxidative stress parameters in the blood and synovial fluid of knee joints of rabbits exposed to varying periods of immobilization. Twenty-eight mature New Zealand albino male rabbits were divided into four groups equal in number. In three groups, the knees were immobilized with a rigid cast for 3, 6 and 9 weeks, respectively. The cartilaginous tissue of the femoral condyles and tibial plateau were analyzed with respect to total count, total volume, and numerical density of chondrocytes using stereohistological methods. Antioxidant activities of superoxide dismutase (SOD), catalase (CAT), and glutathion peroxidase (GSH-Px), and oxidative stress parameters including nitric oxide (NO) and malondialdehyde (MDA) levels were measured in the plasma and synovial fluid. Compared to the control group, total count and total volume of chondrocytes in the femoral condyle and tibial plateau showed significant decreases, while numerical density showed a significant increase at 3 weeks of immobilization. Subsequent immobilization periods resulted in significant decreases in all these parameters, being most remarkable compared to the control group at the end of nine weeks (p<0.001). In plasma and knee joint synovial fluid, all antioxidant enzyme activities (SOD, CAT, and GSH-Px) and oxidative stress parameters (NO and MDA) showed consistent increases compared to the control group throughout the immobilization period (p<0.001). Increased levels of ROS in the blood and synovial fluid might result in cartilage destruction and ROS may be one of the potential factors involved in the etiopathogenesis of osteoarthritis. Prolonged joint immobilization should be avoided in the treatment of orthopedic diseases.
    acta orthopaedica et traumatologica turcica 01/2004; 43(5):436-43. · 0.60 Impact Factor
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    ABSTRACT: The pathogenesis of Behçet's disease (BD) may be related to excessive production of reactive oxygen species, activated neutrophils, and T lymphocytes. The goal of this prospective study was to investigate whether there is any relationship among the oxidant/antioxidant system and nitric oxide (NO) and malondialdehyde (MDA) levels in patients with BD and its subtypes: complete Behçet's disease (CBD) and incomplete Behçet's disease (ICBD), with or without ocular disease. Thirty-two patients and 26 age- and sex-matched healthy control subjects were evaluated for NO and MDA levels and antioxidant enzyme activities. The patients with BD were divided into two subgroups: those with and without ocular disease. Twelve patients with CBD and 4 patients with ICBD had ocular disease. The serum NO level was determined by Griess reaction. The MDA level was detected by thiobarbituric acid reaction. Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in serum were analyzed with spectrophotometric methods. Increased MDA levels but decreased GSH-Px activities in plasma were observed in BD patients with all subtypes, as compared with controls. Concerning the presence of ocular disease and the subtype (CBD or ICBD) compared with each other, there were no significant differences in MDA or NO serum levels and SOD or GSH-Px enzyme activities. Serum NO levels and SOD enzyme activities were not significantly changed in patients with BD and its subtypes; however, a remarkable decrease of GSH-Px enzyme activity and increase of MDA levels were found.
    Ophthalmic Research 01/2004; 36(3):177-82. · 1.56 Impact Factor
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    ABSTRACT: The aim of this study was to investigate the optimum dosage of erdosteine to ameliorate cisplatin-induced nephrotoxicity. Three different doses of erdosteine at 25, 50 and 75 mg kg(-1) were studied in rats. Intraperitoneal administration of 7 mg kg(-1) cisplatin led to acute renal failure, as indicated by kidney histology and increases in plasma creatinine and blood urea nitrogen (BUN) levels. At 5 days after cisplatin injection the BUN level was increased significantly from 15.1 +/- 4.3 to 126.7 +/- 152.6 mg dl(-1) and plasma creatinine levels increased from 0.37 +/- 0.005 to 1.68 +/- 1.9 mg dl(-1). When the rats were administered 50 and 75 mg kg(-1) erdosteine 24 h before cisplatin injection that was continued until sacrifice (total of 6 days), the BUN and creatinine levels remained similar to control levels and the grade of histology was similar. Erdosteine at doses of 50 and 75 mg kg(-1) ameliorates cisplatin-induced renal failure. The optimum dose of erdosteine may be 50 mg kg(-1) in this study.
    Journal of Applied Toxicology 01/2004; 24(4):269-75. · 2.60 Impact Factor

Publication Stats

464 Citations
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48.03 Total Impact Points

Institutions

  • 2003–2013
    • Gaziosmanpasa University
      • Faculty of Medicine
      Dazimon, Tokat, Turkey
    • Firat University
      • Department of Anatomy
      Mezreh, Elazığ, Turkey
  • 2008
    • Mustafa Kemal University
      • Medical Faculty
      Antalya, Antalya, Turkey
    • Gazi University
      • Department of Biochemistry
      Engüri, Ankara, Turkey
  • 2007
    • Inonu University
      • Department of Otolaryngology
      Malatia, Malatya, Turkey
  • 2004
    • Louisiana State University Health Sciences Center New Orleans
      • Department of Ophthalmology
      New Orleans, LA, United States
  • 2002
    • T.C. Süleyman Demirel Üniversitesi
      • Faculty of Medicine
      Hamitabat, Isparta, Turkey