Kwang-Gill Lee

National Academy of Agricultural Science (South Korea), Sŏul, Seoul, South Korea

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Publications (87)72.15 Total impact

  • 03/2015; 30(1):17-25. DOI:10.7852/ijie.2015.30.1.17
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    ABSTRACT: The increased proliferation and migration of vascular smooth muscle cells (VSMC) are key process in the development of atherosclerosis lesions. Platelet-derived growth factor (PDGF) initiates a multitude of biological effects that contribute to VSMC proliferation and migration. Apamin, a component of bee venom, has been known to block the Ca(2+)-activated K(+) channels. However, the effects of apamin in the regulation PDGF-BB-induced VSMC proliferation and migration has not been identified. In this study, we investigate the inhibitory effect of apamin on PDGF-BB-induced VSMC proliferation and migration. Apamin suppressed the PDGF-BB-induced VSMC proliferation and migration with no apparent cytotoxic effect. In accordance with these findings, apamin induced the arrest of cell cycle progression at G0/G1 phase. Apamin also decreased the expressions of G0/G1 specific regulatory proteins including proliferating cell nuclear antigen (PCNA), cyclin D1, cyclin-dependent kinases (CDK) 4, cyclin E and CDK2, as well as increased the expression of p21(Cip1) in PDGF-BB-induced VSMC. Moreover, apamin inhibited PDGF-BB-induced phosphorylation of Akt and Erk1/2. These results suggest that apamin plays an important role in prevention of vascular proliferation and migration through the G0/G1 cell cycle arrest by PDGF signaling pathway. Thus, apamin may be a promising candidate for the therapy of atherosclerosis. Copyright © 2015. Published by Elsevier Inc.
    Vascular Pharmacology 02/2015; 70. DOI:10.1016/j.vph.2014.12.004 · 4.62 Impact Factor
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    ABSTRACT: The objective of this study was to evaluate bone regeneration ability using different concentrations of tetracycline (TC) loaded into silk fibroin membranes (SFMs). Prior to animal experiments, MTT assays and alkaline phosphatase (ALP) assays were performed to evaluate the cellular response of each membrane. Critical sized bone defects (8-mm diameter) on rat calvaria were prepared and covered with SFM containing different concentrations of TC:1% TC (TC1), 5% TC (TC5), 10% TC (TC10), and 0% TC (SFM only). The bone regeneration was evaluated by micro-computerized tomography (mu-CT) and histomorphometric analysis at 4 weeks postoperatively. ALP activity was increased in a dose-dependent manner as the applied TC concentrations. By mu-CT analysis, newly formed bone volume in the TC5 group was significantly higher than that in the SFM only group (P<0.001), the TC1 group (P=0.004), and the TC10 group (P=0.012). From histomorphometric analysis, new bone formation was greater in the TC5 group than in the SFM only group (P=0.003) and the TC1 group (P=0.010). There was no significant difference between the TC5 and TC10 group (P>0.05). In conclusion, TC-loaded SFM showed more bone formation than SFM without TC, and the amount of new bone formation was dependent on TC concentrations.
    Fetal ovine model for in-situ esophagus tissue engineering 11/2014; 11(6). DOI:10.1007/s13770-014-9057-3 · 0.61 Impact Factor
  • 10/2014; 52(2):159-164. DOI:10.7852/jses.2014.52.2.159
  • 10/2014; 52(2):89-95. DOI:10.7852/jses.2014.52.2.89
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    ABSTRACT: Propionibacterium acnes (P. acnes) is a major contributing factor to the inflammatory component of acne. The many prescription medications for acne allow for a large number of potential combination treatments. However, several antibiotics, apart from their antibacterial effects, exert side‑effects, such as the suppression of host inflammatory responses. Purified bee venom (BV) is a natural toxin produced by honeybees (Apis mellifera L.). BV has been widely used as a traditional medicine for various diseases. In the present study, to investigate the therapeutic effects of BV against P. acnes-induced inflammatory skin disease, P. acnes was intradermally injected into the ears of mice. After the injection, BV was applied to the skin surface of the right ear. Histological observation revealed that P. acnes induced a considerable increase in the number of infiltrated inflammatory cells. However, treatment with BV markedly reduced these reactions compared with the P. acnes-injected mice not treated with BV. Moreover, the expression levels of tumor necrosis factor (TNF)-α, and interleukin (IL)-1β were significantly reduced in the BV-treated mice compared with the untreated P. acnes-injected mice. In addition, treatment with BV significantly inhibited Toll-like receptor (TLR)2 and CD14 expression in P. acnes-injected tissue. The binding activity of nuclear factor-κB (NF-κB) and activator protein (AP)-1 was markedly suppressed following treatment with BV. The results from our study, using an animal model, indicate that BV exerts an inhibitory effect on inflammatory skin diseases. In conclusion, our data indicate that BV has potential for use as an anti-acne agent and may be useful in the pharmaceutical and cosmetics industries.
    International Journal of Molecular Medicine 09/2014; 34(5). DOI:10.3892/ijmm.2014.1933 · 1.88 Impact Factor
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    05/2014; 36(3):111-115. DOI:10.14402/jkamprs.2014.36.3.111
  • 04/2014; 52(1):59-63. DOI:10.7852/jses.2014.52.1.59
  • 04/2014; 52(1):52-58. DOI:10.7852/jses.2014.52.1.52
  • 04/2014; 52(1):10-15. DOI:10.7852/jses.2014.52.1.10
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    ABSTRACT: Breast cancer is one of the most common cancers among women worldwide. Recently anticancer agents have been developed using natural substances. To evaluate the anticancer effect of hydrolysates of silk fibroin (HSF), we investigated the effect of HSF on cell viability and apoptosis of a breast cancer cell line, MCF-7, induced through the mitochondrial pathway. The result showed that HSF decreased cell viability in MCF-7 cells in a dose- and time-dependent manner, resulting in an increase in the sub-G1 phase cell population. HSF increased the level of the pro-apoptotic Bax protein and decreased the levels of the anti-apoptotic Bcl-2 protein. In addition, HSF induced apoptosis in MCF-7 cells through a mitochondria-dependent pathway by increasing levels of cytochtome c, and cleavage of PARP. Taken together, these findings suggest that HSF inhibits the proliferation of MCF-7 breast cancer cells through a mitochondria and caspase dependent apoptotic pathway.
    12/2013; 27(2). DOI:10.7852/ijie.2013.27.2.228
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    ABSTRACT: In this study, matured silkworm hemolymph was studied for inhibitory effect on melanin formation, tyrosinase activities. A matured silkworm hemolymph mass purification method was developed and some of suppressing melanin formation effects were investigated, too. As a result, the advanced purification method was higher in yield rate than existing one by 53%. A free radical scavenging activity of hemolymph was higher than sericin on the market(, ) and lower than vitamin C() by . An inhibitory effect on melanin synthesis was higher in hemolymph compared to Morus bark and arbutin by 9.15% and 11.56%, respectively. An inhibitory effect on tyrosinase activity was higher in hemolymph than Morus bark and lower than arbutin. Effect of hemolymph on suppressing of tyrosinase was almost 90%. Thus, these results suggest that hemolymph of matured silkworm may have beneficial properties as a material for cosmetics.
    11/2013; 51(2). DOI:10.7852/jses.2013.51.2.207
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    ABSTRACT: Silk fibroin is a natural biomaterial that has the biocompatibility and other many advantages. But as a silk fibroin membrane thickness increases, the transparency becomes more opaque. Because the transparency of membranes tissue such as the cornea and dura mater are necessary, transparent membrane is required to replace these transparent membranes. In this study, we fabricated blending silk fibroin membranes that made by mixing the various inorganic salts or polymer in an aqueous solution of silk fibroin. The transparency of the membranes were analyzed. the transparency of these membranes is very different, depending on the mixed materials. Inorganic salts mixed silk membrane was more transparent than the polymer mixed one. Especially, the silk fibroin membrane with calcium chloride was very transparent. We showed the possibility of blending silk fibroin membrane, which can be used in perfect transparent membrane such as the cornea. In the future, we expect that the transparent blending silk fibroin membrane can be used in various medical applications.
    11/2013; 51(2). DOI:10.7852/jses.2013.51.2.197
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    ABSTRACT: The objective of this study was to demonstrate that a silk fibroin (SF) and 4-hexylresorcinol (4-HR) incorporation membrane could be used for a guided bone regeneration technique. Fourier transform infrared measurements were obtained to determine change of physical property of SF membrane by 4-HR incorporation. Two peri-implant defects, 3.0 × 5.0 mm (width × length), were prepared on the lateral side of the implant hole in the tibia of New Zealand white rabbits (n = 8). The peri-implant defect was left unfilled in the control group. Silk fibroin + 4-HR membrane was applied to the peri-implant defect in the experimental group. The 8 animals were killed at 8 weeks after implantation. Subsequently, removal torque test and histomorphometric evaluation were done. Fourier transform infrared spectroscopy showed no specific chemical interaction between 4-HR and SF. In the histomorphometric analysis, the mean bone regeneration was 18.3 ± 1.9 mm in the experimental group and 9.3 ± 0.9 mm in the control group (P = 0.004). In conclusion, the SF and 4-HR incorporation membrane successfully regenerated bone in the rabbit tibia peri-implant bone defect model.
    The Journal of craniofacial surgery 11/2013; 24(6):1927-30. DOI:10.1097/SCS.0b013e3182a3050c · 0.68 Impact Factor
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    ABSTRACT: Fibroin and sericin are natural proteins obtained from cocoon and one of the spotlight materials for medical device. Medical device made of these proteins also has the advantage that this material is biodegradable in to amino acid. In this study, we prepared silk sponges using fibroin, sericin and additives. The characterizations of the silk sponges such as morphology, stability, and blood absorbency were observed. The structural stability of the silk sponge decreased significantly by increasing sericin contents. The effect on the concentrations of ethanol to induce crystallization was observed to be superior to 70% ethanol. Structural stability of silk sponges containing additives was very lower than those not containing additives. The blood absorbency of the silk sponges was found to be excellent, regardless of the composition of sericin and fibroin. The resilient power of these sponges was also very good, in spite of the repeated soaking and drying. Therefore, we expect that the silk sponges can be used medical supplies such as plastic implants and hemostatic cotton.
    04/2013; 51(1). DOI:10.7852/jses.2013.51.1.68
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    ABSTRACT: Antheraea pernyi silkworm is one of typical wild silkworms, which spins a tawny color cocoon. The cocoon has been used as a resource for textile material due to strong chemical stability and good mechanical properties. In this study, to increase the solubility efficiency of A. pernyi silk fibroin, the composition of dissolution solvent were examined. Calcium chloride tertiary system, normally used for dissolution of Bombyx mori silk fibroin, does not act on A. pernyi silk fibroin. Calcium nitrate system dissolves A. pernyi silk fibroin, and calcium nitrate ethanol system do more easily than calcium nitrate system. Amino acid composition of A. pernyi silk fibroin obtained after dissolution is mainly composed of alanine, glycine, and serine. A. pernyi silk fibroin would be used for non-textile applications near future.
    04/2013; 51(1). DOI:10.7852/jses.2013.51.1.15
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    ABSTRACT: Honeybee (Apis mellifera L.) venom (BV) has been traditionally used for the treatment of pain and inflammatory diseases such as itchy skin problems. However, the precise mechanism of BV in ameliorating the scratching behavior is not fully understood. In order to evaluate the effect of BV on atopic dermatitis-related symptoms in mice, we used a mouse skin scratching model induced by compound 48/80. The anti-itch effect of BV was investigated in a compound 48/80-induced mouse scratching behavior model. BALB/c mice were injected intraperitoneally with vehicle (saline 0.9%) or BV (0.01 and 0.1 mg/kg). One hour after treatment, the animals received a subcutaneous injection of compound 48/80. Intraperitoneal administration of BV (0.01 and 0.1 mg/kg) attenuated compound 48/80-induced scratching behaviors. The anti-scratching behavior effect of BV was in proportional to its vascular permeability effects. Treatment with BV also inhibited the degranulation of mast cells and the production of pro-inflammatory cytokines in compound 48/80-treated skin tissues. According to these results, BV may improve atopic dermatitis-related symptoms by inhibiting the mast cell degranulation and pro-inflammatory cytokine expression.
    International journal of clinical and experimental pathology 01/2013; 6(12):2896-903. · 1.78 Impact Factor
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    ABSTRACT: Silk is a natural polymer that has the advantages of the biocompatibility, excellent mechanical strength, low immune rejection, and molding facility. But silk does not dissolve easily in water or general solvent. To investigate the characteristics of silk biological membranes according to dissolving condition of silk fibroin, we made the silk biological membranes using silk fibroin solutions with different amount and dissolving time of silk. The characterizations of the silk biological membranes such as morphology, structure, and mechanical strength were observed. Although each biological membrane has the same fibroin content, there was a significant difference in the thickness and transparency. But there was no significant change in the molecular weight of the silk fibroin solutions and morphology of silk biological membranes. We were established the manufacturing condition for silk fibroin biological membrane. So we expect that the conditions will help in the development of medical supplies in the future.
    12/2012; 50(2). DOI:10.7852/jses.2012.50.2.71
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    ABSTRACT: Heavy metals and residual agricultural chemicals in Bombyx mori silkworm cocoon for nontextile usages were examined. Proximate composition of silkworm cocoon was examined; contents of moisture, crude protein, crude fat, and ash were about 5 ~ 6%, 93 ~ 94%, 0.4 ~ 0.5%, and 0.3 ~ 0.4%, respectively. The Heavy metals were analyzed by ICP/MS after acid digestion. Arsenic, cadmium, and lead were not detected. The average contents of mercury, zinc, and cupric were 0.317, 3.99, and 2.06 mg/kg, respectively. Agricultural chemicals, such as dichlorvos and paraquart also were not detected. Silkworm cocoon may be safe used for nontextile applications.
    12/2012; 50(2). DOI:10.7852/jses.2012.50.2.48
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    ABSTRACT: Bone disease can be associated with bone resorption by osteoclasts, and interest in the development of antiresorptive agents has recently increased. The hydrolysate of silk fibroin has been studied with respect to such biomedical applications. In a previous study, silk fibroin showed indirect inhibitory effects on the differentiation of osteoclasts. To further evaluate the effect of a hydrolysate of silk fibroin on osteoclasts, we investigated the direct effects of the silk fibroin hydrolysate on osteoclastogenesis and apoptosis of osteoclasts induced by receptor activation of nuclear factor κB ligand (RANKL). The silk fibroin hydrolysate inhibited RANKL-induced formation of tartrate-resistant acid phosphatase (TRAP) in RAW 264.7 cells. The inhibitory effect of the silk fibroin hydrolysate resulted in the decreased expression of osteoclast marker genes, such as matrix metalloproteinase-9 (MMP-9), cathepsin-K and calcitonin receptor (CTR). In addition, the silk fibroin hydrolysate blocked the signaling pathways of mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) and expression of transcription factors, such as nuclear factor of activated T cells c1 (NFATc1) and NF-κB. Finally, the silk fibroin hydrolysate induced apoptosis signaling cascades. Taken together, the present results indicate that silk fibroin hydrolysate has antiresorptive activity by both inhibiting osteoclastogenesis and inducing osteoclast apoptosis.
    International Journal of Molecular Medicine 11/2012; 30(5):1203-10. DOI:10.3892/ijmm.2012.1120 · 1.88 Impact Factor

Publication Stats

423 Citations
72.15 Total Impact Points

Institutions

  • 2010–2015
    • National Academy of Agricultural Science (South Korea)
      • Division of Agricultural Biology
      Sŏul, Seoul, South Korea
    • Eulji University
      Daiden, Daejeon, South Korea
  • 2010–2014
    • Ulsan National Institute of Science and Technology
      Urusan, Ulsan, South Korea
  • 2013
    • Catholic University of Daegu
      • Department of Pathology
      Kayō, North Gyeongsang, South Korea
  • 2011
    • RURAL DEVELOPMENT ADMINISTRATION
      Seikan-ri, Chungcheongnam-do, South Korea
  • 2005
    • Chung-Ang University
      • Department of Pharmacy
      Sŏul, Seoul, South Korea
    • Instituto Nacional de Tecnología Agropecuaria
      Buenos Aires, Buenos Aires F.D., Argentina